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Long Non-Coding RNA SNHG6 as a Potential Biomarker for Hepatocellular Carcinoma

Long Non-Coding RNA SNHG6 as a Potential Biomarker for Hepatocellular Carcinoma

Long Non-Coding RNA SNHG6as a Potential Biomarker for Hepatocellular CarcinomaMaryam Tahmasebi Birgani1&Mohammadreza Hajjari2&Arman Shahrisa3&Atefeh Khoshnevisan2&Zahra Shoja1&Paria Motahari4&Baharak Farhangi5Received:27August2016/Accepted:26April2017#Arányi Lajos Foundation2017Abstract Long Non-coding RNAs(lncRNAs)refer to all non-protein coding transcripts longer than200nucle-otides.Their critical roles in different biological path-ways have been already well established.Altered ex-pression of lncRNAs can be involved in the cancer ini-tiation and/or progression.Since patients with hepatocel-lular carcinoma(HCC)are usually diagnosed in late stages,developing diagnostic methods seems to be es-sential.In this study,the expression levels of different lncRNAs were systematically analysed in different ge-nomic and transcriptome datasets.The analyses showed that SNHG6is among the lncRNAs with distinctive dys-regulation of expression and copy number variation in HCC tumors compared with normal tissues.The results also suggest that the dysregulation of SNHG6is highly cancer type specific.Through co-occurrence analyses,we found that SNHG6and its related co-expressed genes on8q are involved in the structural integrity of ribosome and translation.This comprehensive in silico analysis,provides a resource for investigating SNHG6in hepatocellular carcinoma and lays the groundwork for design of next researches.Keywords Hepatocellular carcinoma.Biomarker.Long noncoding RNA.SNHG6.Systematic analysis IntroductionHepatocellular carcinoma(HCC)is one of the most common cancers worldwide[1].The viral infection(HBV and HCV), alcoholism,non-alcoholic fatty liver,and some hereditary metabolic diseases are the main recognized risk factors for HCC[2–5].Since most of the HCC patients are diagnosed at late stages,when medication is no longer effective,the discovery of sensitive and specific biomarkers for early diag-nosis and treatment is of great attention[6].Long non-coding RNAs(lncRNAs)refer to all lengthy functional transcripts which are actively in-volved in numerous biological processes such as regu-lation of transcription,translation,protein localization, and function,as well as orchestration of cellular scaf-fold.Furthermore,lncRNAs can control the cell cycle, differentiation,apoptosis,and DNA repair through the modulation of epigenome[7,8].Owing to these func-tions,it is not surprising if the aberrant expressions of lncRNAs contribute to disease pathogenesis.The altered expression of lncRNAs in different malignancies along with their tissue-specific expression suggests that these long transcripts may be considered as great biomarkers in cancer diagnosis[9–12].The potential role ofMaryam Tahmasebi Birgani,Mohammadreza Hajjari and Arman Shahrisa contributed equally to this work.*Maryam Tahmasebi Birganitahmasebi-ma@ajums.ac.ir*Mohammadreza Hajjarim-hajari@scu.ac.ir1Department of Medical Genetics,School of Medicine,Ahvaz Jundishapur University of Medical Sciences,Ahvaz,Iran2Department of Genetics,Faculty of Sciences,Shahid Chamran University of Ahvaz,Ahvaz,Iran3Department of Molecular Genetics,Faculty of Biosciences,Tarbiat Modares University,Tehran,Iran4Department of Biotechnology,Iranian Research Organization Science&Technology,Tehran,Iran5Cancer Research Center,Tehran University of Medical Sciences, Tehran,IranlncRNAs in liver malignancies has been recently led into promising novel insights in HCC therapeutic strat-egies [13,14].Therefore,more studies are needed to elucidate the role of lncRNAs in HCC.In recent decades,systematic analyses of the genomic,transcriptomic,and proteomic datasets have become powerful tools in the discovery and the validation of tumor markers [15].Due to the lack of enough supporting evidence to asso-ciate the lncRNAs with HCC,the present study was aimed to perform a systematic genotranscriptomic meta-analysis of the issue.We tried to screen different genomic and transcriptomic datasets in order to find the potential of lncRNAs as prognos-tic and diagnostic tools for HCC.The systematic results can help the researchers with further studies on specific lncRNAs in order to develop predictive biomarkers or therapeutic tar-gets.In the current study,we found that SNHG6,PVT1,and GAS5are potential lncRNAs with a significant role in HCC initiation and progression.Materials and MethodsThe Selection of lncRNAs with High Alteration Frequency in HCC SamplesTo investigate the significance of lncRNAs in liver ma-lignancy,we retrieved 189approved lncRNAs from HGNC ( ).All of the genes wereinterrogated into the cBioPortal database ( )[16,17]for geno/transcriptomic analyses.We queried all the samples from TCGA liver hepatocel-lular carcinoma (TCGA,provisional)with RNA-seq v2data (n =373)in our study and considered RNA dys-regulation with Z-score threshold:±2.TCGA data,as one of the major national and international efforts,in-clude the valid comprehensive data derived from large cohorts.Among different HCC data indexed in TCGA,TCGA liver hepatocellular carcinoma constitutes more number of tissues.The lncRNAs which were altered in more than 10%of the patients were considered as B significant lncRNAs ^for further analyses.It should not be forgot-ten that it was important for us to consider the lncRNAs which were altered at both genomic and transcriptomic levels even if the percent of alteration in patients was near to the threshold of 10%.Regarding to these criteria,these lncRNAs included SNHG6,PVT1,and GAS5due to high levels of alter-ation in both genomic and transcriptomic levels.By means of the R package,the frequency of each genetic alteration was calculated among the cases carrying at least one alteration for the desired genes.Additionally,the co-occurrence between genetic alterations was con-sidered for all of the three genes.The Differential Expression of Significant lncRNAs between Tumor and Normal TissuesTCGA RNA-Seq raw data was extracted in R using the cgdsr extension package (/web/packages/cgdsr /)with a threshold of ±2.The data was then presented as Heatmap plot.The selected lncRNAs were examined in several transcriptomic datasets to explore if any significant difference of expression may exist between normal and tumor tissues.The included datasets were Oncomine ( ),Gene Expression Atlas [18–20],Gene Expression Omnibus:GEO (/geo ),Array express (https:///arrayexpress ),and UCSC cancer genome browser (https:// )[21–26]databases.The results with Fold change > 1.5and P -value <0.01between tumor and normal tissues were considered as significant.In the next step,we also considered any correlation for any pair of genes of interest using Pearson ’s method.In strong positive correlation,the linear correlation coefficient (r)is close to +1;while in strong negative,the correlation is close to −1.Fig.1Genomic alterations (Copy number Variations)of lncRNAs among 373patients with hepatocellular carcinoma.The chart was drawn for the genes which were altered in more than 10%of the patients with hepatocellular carcinoma.The data obtained from cBioPortal ( )Birgani M.T.et al.The Association between lncRNAsand the Clinicopathologic Parameters of Hepatocellular CarcinomaUsing the UCSC Cancer Genome Browser,the asso-ciation of different lncRNAs with clinicopathologic parameters (the histological type,pathologically TNM staging,and grade)was evaluated using Student ’s t -test.Besides,the effect of gene expression dysregula-tion on the patient ’s survival was evaluated using the Kaplan-Meier analysis in cBioPortal.The Log-Rank Test P -Value <0.05was considered as statistically significant.The Comparison of the HCC Profile of lncRNAs with the Profiles of Other CancersIn order to evaluate whether significant lncRNAs fol-low an HCC-specific manner,we examined the geno/transcriptomic alteration of these genes in all of the tumor collections of cBioPortal.Thirty cancers with available RNA-seq data were included at this stage,and the expression data corresponding to genes were extracted.The raw data was filtered based on the z-score >+2and <−2.The mean of the expression levels was calculated using R and the data was presented asheatmaps.Fig.2Altered expression of different lncRNAs in hepatocellular carcinoma with Z score >±2.Sixty out of 189genes were altered among 373patients with HCC.The analysis is done by the R statistical software.The raw data was extracted from cBioPortal ( )Long Non-Coding RNA SNHG6as a potential BiomarkerThe Functional Analysis of Selected lncRNAsAmong 373patients with hepatocellular carcinoma,130cases showed alterations for SNHG6,PVT1,and GAS5.Among these 130cases,we evaluated the genes which were co-expressed with significant lncRNAs using Pearson ’s correla-tion analysis.Then,the genes with correlation value >0.70were uploaded into the GSEA dataset ( )to compute the gene set overlaps matrix based on the GO molecular ing the cBioPortal database,we also drew a network to find any potential contribution of the genes which were co-expressed with sig-nificant lncRNAs.Statistical AnalysisAll of the analyses including the t-test,heatmap,and correla-tion analysis were done by the R statistical software and SPSS.In our analysis,the P -values less than 0.05were considered significant.ResultsSignificant lncRNAs with a Potential Role in Liver MalignanciesAlthough we could not find any mutations in lncRNAs of interest,67out of the 189lncRNAs were found to be altered in their copy numbers at least in one patient.LncRNAs,in-cluding CASC8,PCAT1,PVT1,CCAT1,F ALEC,and GAS5,were the genes whose copy numbers were altered in more than 10%of the patients (Fig.1).We also found 60lncRNAs with altered expression patterns at least in 1%of patients (Fig.2).However,SNHG6,PVT1,and GAS5were the lncRNAs with the highest RNA dysregulation among the 373samples of HCC (Fig.3).For further analysis,we focused on SNHG6,PVT1,and GAS5in which both CNVs (Copy number varia-tions)and RNA dysregulation were higher than other lncRNAs.It is necessary to mentioned that we granted an exception for selection of SNHG6due to some reasons;1)among 189lncRNAs,SNHG6allocated the highest score in total alteration regardless of whether alterations occur atge-Fig.3The expression levels of SNHG6,PVT1,and GAS5among 373patients the patients with hepatocellular carcinoma. a.The chart shows the genes which were altered in more than 10%of the patients with hepatocellular carcinoma. b.The expression levels of SNHG6,PVT1,and GAS5among 130patients with Z score >±2was presented as heatmap.These patients have at least on alteration in SNHG6,PVT1,and GAS5lncRNAs.The analysis is done by the R statistical software.The raw data was extracted from cBioPortal ( )Table 1The frequency of genetic alterations (CNV)of SNHG6,PVT1and GAS5among 373cases of hepatocellular carcinoma using R analysisGAS5SNHG6PVT1Not altered Duplication Not altered Duplication Not altered Duplication Homodeletion 3373634033307651Birgani M.T.et al.nomic or transcriptomic level.2)among 189lncRNAs,SNHG6allocated the highest score at transcriptomic level so although SNHG6apportioned the 9%alteration among the patients at genomic level but due to the two previous reasons,we decided to continue our analysis on it as well as PVT1and GAS5.On the other hand,when we compared the data at both level of genomic and transcriptomic,SNHG6,PVT1and GAS5were common in data of two groups.Regarding to your true comments,we explain clearly the reason of SNHG6selection (in spite of 9%alteration)in the manuscript.In our results,around 35%of the cases (n =130)had an RNA dysregulation in at least one gene,and SNHG6was altered in more cases than PVT1and GAS5.We called these patients B target samples ^in the next steps.The co-occurrence of different genetic alterations for each paired loci was calcu-lated among this group (Table 1).SNHG6,PVT1,and GAS5are Differentially Expressed between Tumor and Normal TissuesAccording to the Cancer Genome Browser,SNHG6,PVT1,and GAS5are significantly upregulated in hepatocellular car-cinoma in comparison with the normal counterparts (P -val-ue =0.001)(Fig.4).Furthermore,the oncomine datasets con-firmed the differential expression of these lncRNAs between cancerous and normal tissues (Table 2).The Overexpression of SNHG6is a Novel Indicator of Reduced Survival in Patients with Hepatocellular CarcinomaWe found that the over-expression of SNHG6in hepato-cellular carcinoma was nearly associated with reduced survival to a median of 19.74months in SNHG6over-Fig.4TCGA hepatocellular carcinoma (LIHC)gene expression by RNA seq (IlluminaHiseq N =423)and the differential expression of SNHG6,PVT1,and GAS5betweenthe primary tumor and the solid normal tissue.The statistical track displayed under the genomic heatmap shows the logarithmic plot of p -values for each genomic position,where the center line indicates a p -value of 1.The primary tumor tissue and the solid normal tissue subgroups were illustrated in red and greenrespectively.The student t -Test was performed to analyze the differential-ly expressed genes between the tumor cells and the normal ones,and P <0.05was considered statistically significant.The red bar indicates that the expression levels of genes are significantly higher in cancerous cells than normal ones.The data was extracted from Cancer Genome BrowserTable 2The differential expression of lncRNAs SNHG6,PVT1and GAS5between normal and tumour samples (Oncomine,the gene expression atlas,and GEO)lncRNA Fold change P value Down-regulatedUp-regulated Experiment typeRef SNHG6 1.983 3.43E-5Non-Tumour Tissue (n =10)HCC (n =35)Human Genome U133Plus 2.0Array [27]PVT1 4.258 1.88E-11Non-Tumour Tissue (n =10)HCC (n =35)Human Genome U133Plus 2.0Array [27]GAS52.692.3E-8Non-Tumour Tissue (n =10)HCC (n =35)Human Genome U133Plus 2.0Array[27]Long Non-Coding RNA SNHG6as a potential Biomarkerexpressing cases,compared with the period of over 48.95months for the remaining cases (Logrank Test P -Value:0.0558)(Fig.5a ).Although the value was not sig-nificant for PVT1and GAS5,studying the clinical associ-ations of these lncRNAs with clinicopathologic parame-ters of HCC represented that all the three genes (SNHG6,PVT1and GAS5)were significantly expressed in high-grade HCC samples compared with low-grade tissues (P -value =0.001)(Fig.5b ).The Association of SNHG6,PVT1,and GAS5with the Progression of Other Human CancersWe evaluated the transcriptomic alterations of these genes among different human tumors.We considered the genes at two levels;first,their frequency among the patients and sec-ond,their related expression among them.In comparison with PVT1and GAS5,SNHG6allocated a good value to itself at both levels.Therefore,SNHG6seems to be astrongerFig.5Study of the clinicalassociation of SNHG6,PVT1,and GAS5with the clinicopathologic parameters of hepatocellular carcinoma.a Kaplan-Meier plots comparing the overall survival in cases with or without SNHG6over-expression.The data was recruited from cBioPortal.b The association of expression of lncRNAs with the histological grade of HCC;we divided tissues based on their grade (G1–2and G3–4as green and red bars re-spectively),which shown in right column.The statistical track which is displayed under the ge-nomic heatmap shows the loga-rithmic plot of p -values for each lncRNA,where the centreline in-dicates a p -value of 1.The bar above the line indicates that the red subgroup (G3–4)is greater than the green subgroup (P -val-ue <0.05).The patients with no pathological data (NA)were omitted from the analysis.The data was extracted from Cancer Genome Browser,TCGA LIHC gene expression by RNA seq (IlluminaHiseq n =423)Birgani M.T.et al.potential biomarker for liver hepatocellular carcinoma in com-parison with PVT1and GAS5.However,PVT1and GAS5can be useful for other cancers such as Uterine Carcinosarcoma (Fig.6).SNHG6is Possibly Involved in the Structural Integrity of Ribosome and TranslationThrough co-occurrence analyses,we found that SNHG6and GAS5have a tendency toward co-occurrence among target samples (n =130),although it was not significant (Table 3).We considered all the genes which had been co-expressed with SNHG6and GAS5.Pearson value >0.7was taken as the puting the gene set overlaps matrix based on the GO molecular function showed that 13of these genes act as molecules contrib-uting to the structural integrity of the ribosome (Table 4).Interestingly,drawn from cBioPortal data,we found that 35%(n =130)of the patients among the target samples had the alteration at least for one of these genes.Among the genes,RPL8,TOP1MT,and RPL30were the top ones which were altered among the patients.We then visualized SNHG6,its co-expressed genes,and the most frequently altered neigh-bour genes network to investigate any probable mode of interaction.Although we did not observe any interaction between SNHG6and these genes,most of the high fre-quently altered genes were located on 8q,near the SNHG6locus (data not shown).DiscussionAlthough the dysregulation of lncRNAs has been report-ed in some studies,their functional mechanism remains to be challenging.Here is a report consideringtheFig.6The genetic alterations of SNHG6,PVT1,and GAS5amongdifferent human cancers.a The frequency of SNHG6,PVT1and GAS5genetic alterations among the patients of 30cancers with available RNA-seq on the portal.b The heatmap of the mean expression levels of SNHG6,PVT1and GAS5among 30cancers with RNA-seq.The heatmaps were drawn using the R software and the raw data of cBioPortal.The grey column represents cancer with no data in case of the gene of interest in the portalTable 3The mutual exclusivity analysis of SNHG6,PVT1and GAS5among 130tumor samples with alterations at least in one gene.The data was recruited from cBioPortal Gene Pairp -V alue Log Odds Ratio AssociationSNHG6PVT1<0.001−1.821Tendency towards mutual exclusivity (significant)SNHG6GAS5<0.0010.344Tendency towards co-occurrencePVT1GAS5<0.001−0.189Tendency towards mutual exclusivityLong Non-Coding RNA SNHG6as a potential Biomarkercontribution of lncRNAs to hepatocellular carcinoma using available bioportals.We queried189approved lncRNAs in cancer gene expression datasets.It was ob-served that the expression levels of68lncRNAs were altered at least in one case.We chose SNHG6,PVT1, and GAS5as lncRNAs with high levels of alteration. We also observed that SNHG6,PVT1,and GAS5were differentially expressed between the HCC tumors tissues and normal tissues.It was also found that SNHG6allo-cated the most RNA dysregulation in cancerous tissues. Although all the three genes were associated with high grades of HCC,the SNHG6up-regulation was more correlated with the shorter survival of patients. However,this value was on the borderline of the statis-tical significance.In a report by Liu et al.,the potential involvement of of SNHG6in portal vein tumor throm-bus,tumor stage,metastasis,and the shorter overall sur-vival of HCC patients was experimentally confirmed [28].The expression and mutation analyses of desired lncRNAs in other human tumours showed the SNHG6 as a potential biomarker.To study the possible function of SNHG6,we recruited all the genes that were co-expressed with it.We classified the co-expressed genes based on the GO molecular function.Interestingly,we found that some of these genes were involved in ribo-some structure/translation and altered in around35%of our analysed HCC samples.Additionally,we found that most of the high frequently altered genes were located on8q21–24,near the SNHG6locus.This data showed that8q may be associated with HCC.There are merely a few studies showing the role of some ribosomal genes including RP36A,RP44in HCC progression [29].It seems that ribosomal proteins are capable to control the gene expression by preparing a selectivity for translating ribosomes[30].The role of the chromosomal alteration,espe-cially8q24in HCC samples,has been the subject of several studies[31].It has been confirmed that this region encodes several lncRNAs involved in tumorogenesis[32].Altogether, this data introduced SNHG6as a good candidate for experi-mental works in HCC researches.However,clinical experi-ments are urgent to evaluate the molecular role of SNHG6in HCC progression as well as its specificity and sensitivity as a biomarker of HCC.Acknowledgments This study was affiliated to Ahvaz Jundishapur University of Medical Sciences and Shahid Chamran University of Ahvaz,Iran.Compliance with Ethical StandardsConflict of Interest The authors declare no conflict of interest. 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long non-coding RNAs:paradigms with potential roles in cancer.Tumor Biol35:10645–10663Table4TheGeneSet analysis of the genes that are commonly co-expressed with SNHG6and GAS5,based on the GO molecular function.The data was extracted from GSEAGene Set Name Genes in GeneSet(K)Description Genes inOverlap(k)k/K p-value FDR q-valueStructural constituent of ribosome 80Genes annotated by the GO term GO:0003735The action of a molecule thatcontributes to the structural integrityof the ribosome130.1625 3.37E-26 1.33E-2Structural molecule activity244Genes annotated by the GO term GO:0005198The action of a molecule thatcontributes to the structural integrityof a complex or assembly within oroutside a cell130.0533 1.21E-19 2.40E-17RNA Binding259Genes annotated by the GO term GO:0003723Interacting selectively with an RNAmolecule or a portion thereof 60.0309 1.64E-10 2.17E-08Birgani M.T.et al.8.Yarmishyn AA,Kurochkin IV(2015)Long noncoding RNAs:apotential novel class of cancer biomarkers.Front 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potential Biomarker。

Schlumberger - Well Integrity Program 斯伦贝谢井眼完整性程序

Schlumberger - Well Integrity Program 斯伦贝谢井眼完整性程序
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Analysis of Genetic Diversity and Population Structure

Analysis of Genetic Diversity and Population Structure

Agricultural Sciences in China2010, 9(9): 1251-1262September 2010Received 30 October, 2009 Accepted 16 April, 2010Analysis of Genetic Diversity and Population Structure of Maize Landraces from the South Maize Region of ChinaLIU Zhi-zhai 1, 2, GUO Rong-hua 2, 3, ZHAO Jiu-ran 4, CAI Yi-lin 1, W ANG Feng-ge 4, CAO Mo-ju 3, W ANG Rong-huan 2, 4, SHI Yun-su 2, SONG Yan-chun 2, WANG Tian-yu 2 and LI Y u 21Maize Research Institute, Southwest University, Chongqing 400716, P.R.China2Institue of Crop Sciences/National Key Facility for Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences,Beijing 100081, P.R.China3Maize Research Institute, Sichuan Agricultural University, Ya’an 625014, P.R.China4Maize Research Center, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100089, P.R.ChinaAbstractUnderstanding genetic diversity and population structure of landraces is important in utilization of these germplasm in breeding programs. In the present study, a total of 143 core maize landraces from the South Maize Region (SR) of China,which can represent the general profile of the genetic diversity in the landraces germplasm of SR, were genotyped by 54DNA microsatellite markers. Totally, 517 alleles (ranging from 4 to 22) were detected among these landraces, with an average of 9.57 alleles per locus. The total gene diversity of these core landraces was 0.61, suggesting a rather higher level of genetic diversity. Analysis of population structure based on Bayesian method obtained the samilar result as the phylogeny neighbor-joining (NJ) method. The results indicated that the whole set of 143 core landraces could be clustered into two distinct groups. All landraces from Guangdong, Hainan, and 15 landraces from Jiangxi were clustered into group 1, while those from the other regions of SR formed the group 2. The results from the analysis of genetic diversity showed that both of groups possessed a similar gene diversity, but group 1 possessed relatively lower mean alleles per locus (6.63) and distinct alleles (91) than group 2 (7.94 and 110, respectively). The relatively high richness of total alleles and distinct alleles preserved in the core landraces from SR suggested that all these germplasm could be useful resources in germplasm enhancement and maize breeding in China.Key words :maize, core landraces, genetic diversity, population structureINTRODUCTIONMaize has been grown in China for nearly 500 years since its first introduction into this second biggest pro-duction country in the world. Currently, there are six different maize growing regions throughout the coun-try according to the ecological conditions and farming systems, including three major production regions,i.e., the North Spring Maize Region, the Huang-Huai-Hai Summer Maize Region, and the Southwest MaizeRegion, and three minor regions, i.e., the South Maize Region, the Northwest Maize Region, and the Qingzang Plateau Maize Region. The South Maize Region (SR)is specific because of its importance in origin of Chi-nese maize. It is hypothesized that Chinese maize is introduced mainly from two routes. One is called the land way in which maize was first brought to Tibet from India, then to Sichuan Province in southwestern China. The other way is that maize dispersed via the oceans, first shipped to the coastal areas of southeast China by boats, and then spread all round the country1252LIU Zhi-zhai et al.(Xu 2001; Zhou 2000). SR contains all of the coastal provinces and regions lie in southeastern China.In the long-term cultivation history of maize in south-ern China, numerous landraces have been formed, in which a great amount of genetic variation was observed (Li 1998). Similar to the hybrid swapping in Europe (Reif et al. 2005a), the maize landraces have been al-most replaced by hybrids since the 1950s in China (Li 1998). However, some landraces with good adapta-tions and yield performances are still grown in a few mountainous areas of this region (Liu et al.1999). Through a great effort of collection since the 1950s, 13521 accessions of maize landraces have been cur-rently preserved in China National Genebank (CNG), and a core collection of these landraces was established (Li et al. 2004). In this core collection, a total of 143 maize landrace accessions were collected from the South Maize Region (SR) (Table 1).Since simple sequence repeat ( SSR ) markers were firstly used in human genetics (Litt and Luty 1989), it now has become one of the most widely used markers in the related researches in crops (Melchinger et al. 1998; Enoki et al. 2005), especially in the molecular characterization of genetic resources, e.g., soybean [Glycine max (L.) Merr] (Xie et al. 2005), rice (Orya sativa L.) (Garris et al. 2005), and wheat (Triticum aestivum) (Chao et al. 2007). In maize (Zea mays L.), numerous studies focusing on the genetic diversity and population structure of landraces and inbred lines in many countries and regions worldwide have been pub-lished (Liu et al. 2003; Vegouroux et al. 2005; Reif et al. 2006; Wang et al. 2008). These activities of documenting genetic diversity and population structure of maize genetic resources have facilitated the under-standing of genetic bases of maize landraces, the utili-zation of these resources, and the mining of favorable alleles from landraces. Although some studies on ge-netic diversity of Chinese maize inbred lines were con-ducted (Yu et al. 2007; Wang et al. 2008), the general profile of genetic diversity in Chinese maize landraces is scarce. Especially, there are not any reports on ge-netic diversity of the maize landraces collected from SR, a possibly earliest maize growing area in China. In this paper, a total of 143 landraces from SR listed in the core collection of CNG were genotyped by using SSR markers, with the aim of revealing genetic diver-sity of the landraces from SR (Table 2) of China and examining genetic relationships and population struc-ture of these landraces.MATERIALS AND METHODSPlant materials and DNA extractionTotally, 143 landraces from SR which are listed in the core collection of CNG established by sequential strati-fication method (Liu et al. 2004) were used in the present study. Detailed information of all these landrace accessions is listed in Table 1. For each landrace, DNA sample was extracted by a CTAB method (Saghi-Maroof et al. 1984) from a bulk pool constructed by an equal-amount of leaves materials sampled from 15 random-chosen plants of each landrace according to the proce-dure of Reif et al. (2005b).SSR genotypingA total of 54 simple sequence repeat (SSR) markers covering the entire maize genome were screened to fin-gerprint all of the 143 core landrace accessions (Table 3). 5´ end of the left primer of each locus was tailed by an M13 sequence of 5´-CACGACGTTGTAAAACGAC-3´. PCR amplification was performed in a 15 L reac-tion containing 80 ng of template DNA, 7.5 mmol L-1 of each of the four dNTPs, 1×Taq polymerase buffer, 1.5 mmol L-1 MgCl2, 1 U Taq polymerase (Tiangen Biotech Co. Ltd., Beijing, China), 1.2 mol L-1 of forward primer and universal fluorescent labeled M13 primer, and 0.3 mol L-1 of M13 sequence tailed reverse primer (Schuelke 2000). The amplification was carried out in a 96-well DNA thermal cycler (GeneAmp PCR System 9700, Applied Biosystem, USA). PCR products were size-separated on an ABI Prism 3730XL DNA sequencer (HitachiHigh-Technologies Corporation, Tokyo, Japan) via the software packages of GENEMAPPER and GeneMarker ver. 6 (SoftGenetics, USA).Data analysesAverage number of alleles per locus and average num-ber of group-specific alleles per locus were identifiedAnalysis of Genetic Diversity and Population Structure of Maize Landraces from the South Maize Region of China 1253Table 1 The detailed information about the landraces used in the present studyPGS revealed by Structure1) NJ dendragram revealed Group 1 Group 2 by phylogenetic analysis140-150tian 00120005AnH-06Jingde Anhui 0.0060.994Group 2170tian00120006AnH-07Jingde Anhui 0.0050.995Group 2Zixihuangyumi00120007AnH-08Zixi Anhui 0.0020.998Group 2Zixibaihuangzayumi 00120008AnH-09Zixi Anhui 0.0030.997Group 2Baiyulu 00120020AnH-10Yuexi Anhui 0.0060.994Group 2Wuhuazi 00120021AnH-11Yuexi Anhui 0.0030.997Group 2Tongbai 00120035AnH-12Tongling Anhui 0.0060.994Group 2Yangyulu 00120036AnH-13Yuexi Anhui 0.0040.996Group 2Huangli 00120037AnH-14Tunxi Anhui 0.0410.959Group 2Baiyumi 00120038AnH-15Tunxi Anhui 0.0030.997Group 2Dapigu00120039AnH-16Tunxi Anhui 0.0350.965Group 2150tianbaiyumi 00120040AnH-17Xiuning Anhui 0.0020.998Group 2Xiuning60tian 00120042AnH-18Xiuning Anhui 0.0040.996Group 2Wubaogu 00120044AnH-19ShitaiAnhui 0.0020.998Group 2Kuyumi00130001FuJ-01Shanghang Fujian 0.0050.995Group 2Zhongdouyumi 00130003FuJ-02Shanghang Fujian 0.0380.962Group 2Baixinyumi 00130004FuJ-03Liancheng Fujian 0.0040.996Group 2Hongxinyumi 00130005FuJ-04Liancheng Fujian 0.0340.966Group 2Baibaogu 00130008FuJ-05Changding Fujian 0.0030.997Group 2Huangyumi 00130011FuJ-06Jiangyang Fujian 0.0020.998Group 2Huabaomi 00130013FuJ-07Shaowu Fujian 0.0020.998Group 2Huangbaomi 00130014FuJ-08Songxi Fujian 0.0020.998Group 2Huangyumi 00130016FuJ-09Wuyishan Fujian 0.0460.954Group 2Huabaogu 00130019FuJ-10Jian’ou Fujian 0.0060.994Group 2Huangyumi 00130024FuJ-11Guangze Fujian 0.0010.999Group 2Huayumi 00130025FuJ-12Nanping Fujian 0.0040.996Group 2Huangyumi 00130026FuJ-13Nanping Fujian 0.0110.989Group 2Hongbaosu 00130027FuJ-14Longyan Fujian 0.0160.984Group 2Huangfansu 00130029FuJ-15Loangyan Fujian 0.0020.998Group 2Huangbaosu 00130031FuJ-16Zhangping Fujian 0.0060.994Group 2Huangfansu 00130033FuJ-17Zhangping Fujian0.0040.996Group 2Baolieyumi 00190001GuangD-01Guangzhou Guangdong 0.9890.011Group 1Nuomibao (I)00190005GuangD-02Shixing Guangdong 0.9740.026Group 1Nuomibao (II)00190006GuangD-03Shixing Guangdong 0.9790.021Group 1Zasehuabao 00190010GuangD-04Lechang Guangdong 0.9970.003Group 1Zihongmi 00190013GuangD-05Lechang Guangdong 0.9880.012Group 1Jiufengyumi 00190015GuangD-06Lechang Guangdong 0.9950.005Group 1Huangbaosu 00190029GuangD-07MeiGuangdong 0.9970.003Group 1Bailibao 00190032GuangD-08Xingning Guangdong 0.9980.002Group 1Nuobao00190038GuangD-09Xingning Guangdong 0.9980.002Group 1Jinlanghuang 00190048GuangD-10Jiangcheng Guangdong 0.9960.004Group 1Baimizhenzhusu 00190050GuangD-11Yangdong Guangdong 0.9940.006Group 1Huangmizhenzhusu 00190052GuangD-12Yangdong Guangdong 0.9930.007Group 1Baizhenzhu 00190061GuangD-13Yangdong Guangdong 0.9970.003Group 1Baiyumi 00190066GuangD-14Wuchuan Guangdong 0.9880.012Group 1Bendibai 00190067GuangD-15Suixi Guangdong 0.9980.002Group 1Shigubaisu 00190068GuangD-16Gaozhou Guangdong 0.9960.004Group 1Zhenzhusu 00190069GuangD-17Xinyi Guangdong 0.9960.004Group 1Nianyaxixinbai 00190070GuangD-18Huazhou Guangdong 0.9960.004Group 1Huangbaosu 00190074GuangD-19Xinxing Guangdong 0.9950.005Group 1Huangmisu 00190076GuangD-20Luoding Guangdong 0.940.060Group 1Huangmi’ai 00190078GuangD-21Luoding Guangdong 0.9980.002Group 1Bayuemai 00190084GuangD-22Liannan Guangdong 0.9910.009Group 1Baiyumi 00300001HaiN-01Haikou Hainan 0.9960.004Group 1Baiyumi 00300003HaiN-02Sanya Hainan 0.9970.003Group 1Hongyumi 00300004HaiN-03Sanya Hainan 0.9980.002Group 1Baiyumi00300011HaiN-04Tongshi Hainan 0.9990.001Group 1Zhenzhuyumi 00300013HaiN-05Tongshi Hainan 0.9980.002Group 1Zhenzhuyumi 00300015HaiN-06Qiongshan Hainan 0.9960.004Group 1Aiyumi 00300016HaiN-07Qiongshan Hainan 0.9960.004Group 1Huangyumi 00300021HaiN-08Qionghai Hainan 0.9970.003Group 1Y umi 00300025HaiN-09Qionghai Hainan 0.9870.013Group 1Accession name Entry code Analyzing code Origin (county/city)Province/Region1254LIU Zhi-zhai et al .Baiyumi00300032HaiN-10Tunchang Hainan 0.9960.004Group 1Huangyumi 00300051HaiN-11Baisha Hainan 0.9980.002Group 1Baihuangyumi 00300055HaiN-12BaishaHainan 0.9970.003Group 1Machihuangyumi 00300069HaiN-13Changjiang Hainan 0.9900.010Group 1Hongyumi00300073HaiN-14Dongfang Hainan 0.9980.002Group 1Xiaohonghuayumi 00300087HaiN-15Lingshui Hainan 0.9980.002Group 1Baiyumi00300095HaiN-16Qiongzhong Hainan 0.9950.005Group 1Y umi (Baimai)00300101HaiN-17Qiongzhong Hainan 0.9980.002Group 1Y umi (Xuemai)00300103HaiN-18Qiongzhong Hainan 0.9990.001Group 1Huangmaya 00100008JiangS-10Rugao Jiangsu 0.0040.996Group 2Bainian00100012JiangS-11Rugao Jiangsu 0.0080.992Group 2Bayebaiyumi 00100016JiangS-12Rudong Jiangsu 0.0040.996Group 2Chengtuohuang 00100021JiangS-13Qidong Jiangsu 0.0050.995Group 2Xuehuanuo 00100024JiangS-14Qidong Jiangsu 0.0020.998Group 2Laobaiyumi 00100032JiangS-15Qidong Jiangsu 0.0050.995Group 2Laobaiyumi 00100033JiangS-16Qidong Jiangsu 0.0010.999Group 2Huangwuye’er 00100035JiangS-17Hai’an Jiangsu 0.0030.997Group 2Xiangchuanhuang 00100047JiangS-18Nantong Jiangsu 0.0060.994Group 2Huangyingzi 00100094JiangS-19Xinghua Jiangsu 0.0040.996Group 2Xiaojinhuang 00100096JiangS-20Yangzhou Jiangsu 0.0010.999Group 2Liushizi00100106JiangS-21Dongtai Jiangsu 0.0030.997Group 2Kangnandabaizi 00100108JiangS-22Dongtai Jiangsu 0.0020.998Group 2Shanyumi 00140020JiangX-01Dexing Jiangxi 0.9970.003Group 1Y umi00140024JiangX-02Dexing Jiangxi 0.9970.003Group 1Tianhongyumi 00140027JiangX-03Yushan Jiangxi 0.9910.009Group 1Hongganshanyumi 00140028JiangX-04Yushan Jiangxi 0.9980.002Group 1Zaoshuyumi 00140032JiangX-05Qianshan Jiangxi 0.9970.003Group 1Y umi 00140034JiangX-06Wannian Jiangxi 0.9970.003Group 1Y umi 00140038JiangX-07De’an Jiangxi 0.9940.006Group 1Y umi00140045JiangX-08Wuning Jiangxi 0.9740.026Group 1Chihongyumi 00140049JiangX-09Wanzai Jiangxi 0.9920.008Group 1Y umi 00140052JiangX-10Wanzai Jiangxi 0.9930.007Group 1Huayumi 00140060JiangX-11Jing’an Jiangxi 0.9970.003Group 1Baiyumi 00140065JiangX-12Pingxiang Jiangxi 0.9940.006Group 1Huangyumi00140066JiangX-13Pingxiang Jiangxi 0.9680.032Group 1Nuobaosuhuang 00140068JiangX-14Ruijin Jiangxi 0.9950.005Group 1Huangyumi 00140072JiangX-15Xinfeng Jiangxi 0.9960.004Group 1Wuningyumi 00140002JiangX-16Jiujiang Jiangxi 0.0590.941Group 2Tianyumi 00140005JiangX-17Shangrao Jiangxi 0.0020.998Group 2Y umi 00140006JiangX-18Shangrao Jiangxi 0.0310.969Group 2Baiyiumi 00140012JiangX-19Maoyuan Jiangxi 0.0060.994Group 260riyumi 00140016JiangX-20Maoyuan Jiangxi 0.0020.998Group 2Shanyumi 00140019JiangX-21Dexing Jiangxi 0.0050.995Group 2Laorenya 00090002ShangH-01Chongming Shanghai 0.0050.995Group 2Jinmeihuang 00090004ShangH-02Chongming Shanghai 0.0020.998Group 2Zaobaiyumi 00090006ShangH-03Chongming Shanghai 0.0020.998Group 2Chengtuohuang 00090007ShangH-04Chongming Shanghai 0.0780.922Group 2Benyumi (Huang)00090008ShangH-05Shangshi Shanghai 0.0020.998Group 2Bendiyumi 00090010ShangH-06Shangshi Shanghai 0.0040.996Group 2Baigengyumi 00090011ShangH-07Jiading Shanghai 0.0020.998Group 2Huangnuoyumi 00090012ShangH-08Jiading Shanghai 0.0040.996Group 2Huangdubaiyumi 00090013ShangH-09Jiading Shanghai 0.0440.956Group 2Bainuoyumi 00090014ShangH-10Chuansha Shanghai 0.0010.999Group 2Laorenya 00090015ShangH-11Shangshi Shanghai 0.0100.990Group 2Xiaojinhuang 00090016ShangH-12Shangshi Shanghai 0.0050.995Group 2Gengbaidayumi 00090017ShangH-13Shangshi Shanghai 0.0020.998Group 2Nongmeiyihao 00090018ShangH-14Shangshi Shanghai 0.0540.946Group 2Chuanshazinuo 00090020ShangH-15Chuansha Shanghai 0.0550.945Group 2Baoanshanyumi 00110004ZheJ-01Jiangshan Zhejiang 0.0130.987Group 2Changtaixizi 00110005ZheJ-02Jiangshan Zhejiang 0.0020.998Group 2Shanyumibaizi 00110007ZheJ-03Jiangshan Zhejiang 0.0020.998Group 2Kaihuajinyinbao 00110017ZheJ-04Kaihua Zhejiang 0.0100.990Group 2Table 1 (Continued from the preceding page)PGS revealed by Structure 1) NJ dendragram revealed Group1 Group2 by phylogenetic analysisAccession name Entry code Analyzing code Origin (county/city)Province/RegoinAnalysis of Genetic Diversity and Population Structure of Maize Landraces from the South Maize Region of China 1255Liputianzi00110038ZheJ-05Jinhua Zhejiang 0.0020.998Group 2Jinhuaqiuyumi 00110040ZheJ-06Jinhua Zhejiang 0.0050.995Group 2Pujiang80ri 00110069ZheJ-07Pujiang Zhejiang 0.0210.979Group 2Dalihuang 00110076ZheJ-08Yongkang Zhejiang 0.0140.986Group 2Ziyumi00110077ZheJ-09Yongkang Zhejiang 0.0020.998Group 2Baiyanhandipinzhong 00110078ZheJ-10Yongkang Zhejiang 0.0030.997Group 2Duosuiyumi00110081ZheJ-11Wuyi Zhejiang 0.0020.998Group 2Chun’an80huang 00110084ZheJ-12Chun’an Zhejiang 0.0020.998Group 2120ribaiyumi 00110090ZheJ-13Chun’an Zhejiang 0.0020.998Group 2Lin’anliugu 00110111ZheJ-14Lin’an Zhejiang 0.0030.997Group 2Qianhuangyumi00110114ZheJ-15Lin’an Zhejiang 0.0030.997Group 2Fenshuishuitianyumi 00110118ZheJ-16Tonglu Zhejiang 0.0410.959Group 2Kuihualiugu 00110119ZheJ-17Tonglu Zhejiang 0.0030.997Group 2Danbaihuang 00110122ZheJ-18Tonglu Zhejiang 0.0020.998Group 2Hongxinma 00110124ZheJ-19Jiande Zhejiang 0.0030.997Group 2Shanyumi 00110136ZheJ-20Suichang Zhejiang 0.0030.997Group 2Bai60ri 00110143ZheJ-21Lishui Zhejiang 0.0050.995Group 2Zeibutou 00110195ZheJ-22Xianju Zhejiang 0.0020.998Group 2Kelilao00110197ZheJ-23Pan’an Zhejiang 0.0600.940Group 21)The figures refered to the proportion of membership that each landrace possessed.Table 1 (Continued from the preceding page)PGS revealed by Structure 1) NJ dendragram revealed Group 1 Group 2 by phylogenetic analysisAccession name Entry code Analyzing code Origin (county/city)Province/Regoin Table 2 Construction of two phylogenetic groups (SSR-clustered groups) and their correlation with geographical locationsGeographical location SSR-clustered groupChi-square testGroup 1Group 2Total Guangdong 2222 χ2 = 124.89Hainan 1818P < 0.0001Jiangxi 15621Anhui 1414Fujian 1717Jiangsu 1313Shanghai 1515Zhejiang 2323Total5588143by the software of Excel MicroSatellite toolkit (Park 2001). Average number of alleles per locus was calcu-lated by the formula rAA rj j¦1, with the standarddeviation of1)()(12¦ r A AA rj jV , where A j was thenumber of distinct alleles at locus j , and r was the num-ber of loci (Park 2001).Unbiased gene diversity also known as expected heterozygosity, observed heterozygosity for each lo-cus and average gene diversity across the 54 SSR loci,as well as model-based groupings inferred by Struc-ture ver. 2.2, were calculated by the softwarePowerMarker ver.3.25 (Liu et al . 2005). Unbiased gene diversity for each locus was calculated by˅˄¦ 2ˆ1122ˆi x n n h , where 2ˆˆ2ˆ2¦¦z ji ijij i X X x ,and ij X ˆwas the frequency of genotype A i A jin the sample, and n was the number of individuals sampled.The average gene diversity across 54 loci was cal-culated as described by Nei (1987) as follows:rh H rj j ¦1ˆ, with the variance ,whereThe average observed heterozygosity across the en-tire loci was calculated as described by (Hedrick 1983)as follows: r jrj obsobs n h h ¦1, with the standard deviationrn h obs obsobs 1V1256LIU Zhi-zhai et al.Phylogenetic analysis and population genetic structureRelationships among all of the 143 accessions collected from SR were evaluated by using the unweighted pair group method with neighbor-joining (NJ) based on the log transformation of the proportion of shared alleles distance (InSPAD) via PowerMarker ver. 3.25 (FukunagaTable 3 The PIC of each locus and the number of alleles detected by 54 SSRsLocus Bin Repeat motif PIC No. of alleles Description 2)bnlg1007y51) 1.02AG0.7815Probe siteumc1122 1.06GGT0.639Probe siteumc1147y41) 1.07CA0.2615Probe sitephi961001) 2.00ACCT0.298Probe siteumc1185 2.03GC0.7215ole1 (oleosin 1)phi127 2.08AGAC0.577Probe siteumc1736y21) 2.09GCA T0.677Probe sitephi453121 3.01ACC0.7111Probe sitephi374118 3.03ACC0.477Probe sitephi053k21) 3.05A TAC0.7910Probe sitenc004 4.03AG0.4812adh2 (alcohol dehydrogenase 2)bnlg490y41) 4.04T A0.5217Probe sitephi079 4.05AGATG0.495gpc1(glyceraldehyde-3-phosphate dehydrogenase 1) bnlg1784 4.07AG0.6210Probe siteumc1574 4.09GCC0.719sbp2 (SBP-domain protein 2)umc1940y51) 4.09GCA0.4713Probe siteumc1050 4.11AA T0.7810cat3 (catalase 3)nc130 5.00AGC0.5610Probe siteumc2112y31) 5.02GA0.7014Probe sitephi109188 5.03AAAG0.719Probe siteumc1860 5.04A T0.325Probe sitephi085 5.07AACGC0.537gln4 (glutamine synthetase 4)phi331888 5.07AAG0.5811Probe siteumc1153 5.09TCA0.7310Probe sitephi075 6.00CT0.758fdx1 (ferredoxin 1)bnlg249k21) 6.01AG0.7314Probe sitephi389203 6.03AGC0.416Probe sitephi299852y21) 6.07AGC0.7112Probe siteumc1545y21)7.00AAGA0.7610hsp3(heat shock protein 3)phi1127.01AG0.5310o2 (opaque endosperm 2)phi4207018.00CCG0.469Probe siteumc13598.00TC0.7814Probe siteumc11398.01GAC0.479Probe siteumc13048.02TCGA0.335Probe sitephi1158.03A TAC0.465act1(actin1)umc22128.05ACG0.455Probe siteumc11218.05AGAT0.484Probe sitephi0808.08AGGAG0.646gst1 (glutathione-S-transferase 1)phi233376y11)8.09CCG0.598Probe sitebnlg12729.00AG0.8922Probe siteumc20849.01CTAG0.498Probe sitebnlg1520k11)9.01AG0.5913Probe sitephi0659.03CACCT0.519pep1(phosphoenolpyruvate carboxylase 1)umc1492y131)9.04GCT0.2514Probe siteumc1231k41)9.05GA0.2210Probe sitephi1084119.06AGCT0.495Probe sitephi4488809.06AAG0.7610Probe siteumc16759.07CGCC0.677Probe sitephi041y61)10.00AGCC0.417Probe siteumc1432y61)10.02AG0.7512Probe siteumc136710.03CGA0.6410Probe siteumc201610.03ACAT0.517pao1 (polyamine oxidase 1)phi06210.04ACG0.337mgs1 (male-gametophyte specific 1)phi07110.04GGA0.515hsp90 (heat shock protein, 90 kDa)1) These primers were provided by Beijing Academy of Agricultural and Forestry Sciences (Beijing, China).2) Searched from Analysis of Genetic Diversity and Population Structure of Maize Landraces from the South Maize Region of China1257et al. 2005). The unrooted phylogenetic tree was finally schematized with the software MEGA (molecular evolu-tionary genetics analysis) ver. 3.1 (Kumar et al. 2004). Additionally, a chi-square test was used to reveal the correlation between the geographical origins and SSR-clustered groups through FREQ procedure implemented in SAS ver. 9.0 (2002, SAS Institute, Inc.).In order to reveal the population genetic structure (PGS) of 143 landrace accessions, a Bayesian approach was firstly applied to determine the number of groups (K) that these materials should be assigned by the soft-ware BAPS (Bayesian Analysis of Population Structure) ver.5.1. By using BAPS, a fixed-K clustering proce-dure was applied, and with each separate K, the num-ber of runs was set to 100, and the value of log (mL) was averaged to determine the appropriate K value (Corander et al. 2003; Corander and Tang 2007). Since the number of groups were determined, a model-based clustering analysis was used to assign all of the acces-sions into the corresponding groups by an admixture model and a correlated allele frequency via software Structure ver.2.2 (Pritchard et al. 2000; Falush et al. 2007), and for the given K value determined by BAPS, three independent runs were carried out by setting both the burn-in period and replication number 100000. The threshold probability assigned individuals into groupswas set by 0.8 (Liu et al. 2003). The PGS result carried out by Structure was visualized via Distruct program ver. 1.1 (Rosenberg 2004).RESULTSGenetic diversityA total of 517 alleles were detected by the whole set of54 SSRs covering the entire maize genome through all of the 143 maize landraces, with an average of 9.57 alleles per locus and ranged from 4 (umc1121) to 22 (bnlg1272) (Table 3). Among all the alleles detected, the number of distinct alleles accounted for 132 (25.53%), with an av-erage of 2.44 alleles per locus. The distinct alleles dif-fered significantly among the landraces from different provinces/regions, and the landraces from Guangdong, Fujian, Zhejiang, and Shanghai possessed more distinct alleles than those from the other provinces/regions, while those from southern Anhui possessed the lowest distinct alleles, only counting for 3.28% of the total (Table 4).Table 4 The genetic diversity within eight provinces/regions and groups revealed by 54 SSRsProvince/Region Sample size Allele no.1)Distinct allele no.Gene diversity (expected heterozygosity)Observed heterozygosity Anhui14 4.28 (4.19) 69 (72.4)0.51 (0.54)0.58 (0.58)Fujian17 4.93 (4.58 80 (79.3)0.56 (0.60)0.63 (0.62)Guangdong22 5.48 (4.67) 88 (80.4)0.57 (0.59)0.59 (0.58)Hainan18 4.65 (4.26) 79 (75.9)0.53 (0.57)0.55 (0.59)Jiangsu13 4.24 700.500.55Jiangxi21 4.96 (4.35) 72 (68.7)0.56 (0.60)0.68 (0.68)Shanghai15 5.07 (4.89) 90 (91.4)0.55 (0.60)0.55 (0.55)Zhejiang23 5.04 (4.24) 85 (74)0.53 (0.550.60 (0.61)Total/average1439.571320.610.60GroupGroup 155 6.63 (6.40) 91 (89.5)0.57 (0.58)0.62 (0.62)Group 2887.94 (6.72)110 (104.3)0.57 (0.57)0.59 (0.58)Total/Average1439.571320.610.60Provinces/Regions within a groupGroup 1Total55 6.69 (6.40) 910.57 (0.58)0.62 (0.62)Guangdong22 5.48 (4.99) 86 (90.1)0.57 (0.60)0.59 (0.58)Hainan18 4.65 (4.38) 79 (73.9)0.53 (0.56)0.55 (0.59)Jiangxi15 4.30 680.540.69Group 2Total887.97 (6.72)110 (104.3)0.57 (0.57)0.59 (0.58)Anhui14 4.28 (3.22) 69 (63.2)0.51 (0.54)0.58 (0.57)Fujian17 4.93 (3.58) 78 (76.6)0.56 (0.60)0.63 (0.61)Jiangsu13 4.24 (3.22) 71 (64.3)0.50 (0.54)0.55 (0.54)Jiangxi6 3.07 520.460.65Shanghai15 5.07 (3.20) 91 (84.1)0.55 (0.60)0.55 (0.54)Zhejiang23 5.04 (3.20) 83 (61.7)0.53 (0.54)0.60 (0.58)1258LIU Zhi-zhai et al.Among the 54 loci used in the study, 16 (or 29.63%) were dinucleotide repeat SSRs, which were defined as type class I-I, the other 38 loci were SSRs with a longer repeat motifs, and two with unknown repeat motifs, all these 38 loci were defined as the class of I-II. In addition, 15 were located within certain functional genes (defined as class II-I) and the rest were defined as class II-II. The results of comparison indicated that the av-erage number of alleles per locus captured by class I-I and II-II were 12.88 and 10.05, respectively, which were significantly higher than that by type I-II and II-I (8.18 and 8.38, respectively). The gene diversity re-vealed by class I-I (0.63) and II-I (0.63) were some-what higher than by class I-II (0.60) and II-II (0.60) (Table 5).Genetic relationships of the core landraces Overall, 143 landraces were clustered into two groups by using neighbor-joining (NJ) method based on InSPAD. All the landraces from provinces of Guangdong and Hainan and 15 of 21 from Jiangxi were clustered together to form group 1, and the other 88 landraces from the other provinces/regions formed group 2 (Fig.-B). The geographical origins of all these 143 landraces with the clustering results were schematized in Fig.-D. Revealed by the chi-square test, the phylogenetic results (SSR-clustered groups) of all the 143 landraces from provinces/regions showed a significant correlation with their geographical origin (χ2=124.89, P<0.0001, Table 2).Revealed by the phylogenetic analysis based on the InSPAD, the minimum distance was observed as 0.1671 between two landraces, i.e., Tianhongyumi (JiangX-03) and Hongganshanyumi (JiangX-04) collected from Jiangxi Province, and the maximum was between two landraces of Huangbaosu (FuJ-16) and Hongyumi (HaiN-14) collected from provinces of Fujian and Hainan, respectively, with the distance of 1.3863 (data not shown). Two landraces (JiangX-01 and JiangX-21) collected from the same location of Dexing County (Table 1) possessing the same names as Shanyumi were separated to different groups, i.e., JiangX-01 to group1, while JiangX-21 to group 2 (Table 1). Besides, JiangX-01 and JiangX-21 showed a rather distant distance of 0.9808 (data not shown). These results indicated that JiangX-01 and JiangX-21 possibly had different ances-tral origins.Population structureA Bayesian method was used to detect the number of groups (K value) of the whole set of landraces from SR with a fixed-K clustering procedure implemented in BAPS software ver. 5.1. The result showed that all of the 143 landraces could also be assigned into two groups (Fig.-A). Then, a model-based clustering method was applied to carry out the PGS of all the landraces via Structure ver. 2.2 by setting K=2. This method as-signed individuals to groups based on the membership probability, thus the threshold probability 0.80 was set for the individuals’ assignment (Liu et al. 2003). Accordingly, all of the 143 landraces were divided into two distinct model-based groups (Fig.-C). The landraces from Guangdong, Hainan, and 15 landraces from Jiangxi formed one group, while the rest 6 landraces from the marginal countries of northern Jiangxi and those from the other provinces formed an-other group (Table 1, Fig.-D). The PGS revealed by the model-based approach via Structure was perfectly consistent with the relationships resulted from the phy-logenetic analysis via PowerMarker (Table 1).DISCUSSIONThe SR includes eight provinces, i.e., southern Jiangsu and Anhui, Shanghai, Zhejiang, Fujian, Jiangxi, Guangdong, and Hainan (Fig.-C), with the annual maize growing area of about 1 million ha (less than 5% of theTable 5 The genetic diversity detected with different types of SSR markersType of locus No. of alleles Gene diversity Expected heterozygosity PIC Class I-I12.880.630.650.60 Class I-II8.180.600.580.55 Class II-I8.330.630.630.58。

RECSIT1[1].1中英文对照全文

RECSIT1[1].1中英文对照全文

New response evaluation criteria in solid tumours: Revised RECIST guideline (version 1.1)新版实体瘤疗效评价标准:修订的RECIST指南(1.1版本)Abstract摘要Background背景介绍Assessment of the change in tumour burden is an important feature of the clinical evaluation of cancer therapeutics: both tumour shrinkage (objective response) and disease progression are useful endpoints in clinical trials. Since RECIST was published in 2000, many investigators, cooperative groups, industry and government authorities have adopted these criteria in the assessment of treatment outcomes. However, a number of questions and issues have arisen which have led to the development of a revised RECIST guideline (version 1.1). Evidence for changes, summarised in separate papers in this special issue, has come from assessment of a large data warehouse (>6500 patients), simulation studies and literature reviews.临床上评价肿瘤治疗效果最重要的一点就是对肿瘤负荷变化的评估:瘤体皱缩(目标疗效)和病情恶化在临床试验中都是有意义的判断终点。

几种类型的多路复用技术

几种类型的多路复用技术
Data rate of multiplexed line is fixed If one channel receiver can not receive data, the others must carry on The corresponding source must be quenched This leaves empty slots
12 voice channels (4kHz each) = 48kHz Range 60kHz to 108kHz
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Analog Carrier Systems(2)
Supergroup(超群)
60 channel FDM of 5 group signals on carriers between 420kHz and 612 kHz
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Frequency Division Multiplexing(2)
Carrier frequencies separated so signals do not overlap (guard bands) 载波频率要相互隔离使信号不重叠(防护频带) e.g. broadcast radio Channel allocated even if no data
Data and Computer Communications
Chapter 8 Multiplexing
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多路复用
为充分利用通信干线的传输容量,常采用 多路复用技术。多路器(复用器)组合N路 输入线路上的数据,通过一条高容量的数据 链路传输;分路器接收到复用的数据流,按 通道分离(分路)这些数据,把它们送到适当 的输出线路上。
m1(t)
Ssc1(t) 子载波fsc1

JTAG协议规范1149.1和1149.7

JTAG协议规范1149.1和1149.7

Doing More with Less – An IEEE 1149.7 Embedded Tutorial : Standard for Reduced-pin and Enhanced-functionality Test Access Port andBoundary-Scan ArchitectureAdam W LeyASSET InterTech, Inc. Richardson TX, USAAbstractIEEE Std 1149.7 offers a means to reduce chip pins dedicated to test (and debug) access while enhancing the functionality of the Test Access Port (TAP) as a complementary superset of the original IEEE Std 1149.1 (JTAG). Extended features such as hot-plug immunity, power management, optimization of scan throughput, access to instrumentation, and access to custom technologies provide welcome improvements for debug. Further, the boundary-scan architecture is bolstered to ensure full support for test. This important advancement in test and debug interfaces is well suited for access to multiple cores on SOC or multiple die in SIP or POP.1.IntroductionIn the 1980s, the Joint Test Action Group (JTAG) was formed to address a growing concern about diminishing test access to chips on boards due to the adoption of surface-mount assembly methods and ongoing miniaturization of chip packages. In 1990, their efforts culminated in the ratification of IEEE Std 1149.1 – Standard Test Access Port and Boundary-Scan Architecture. While 1149.1 was firmly rooted in the needto solve the problems of board test, as exemplified by the provision for boundary scan, the proponents of the standard realized the need for a generalized means of low-level access to components on boards and in systems that would suit a wide range of uses. As a result, the 1149.1 test access port (TAP), as specified, has met this need.In fact, even before the ink was dry, the 1149.1 TAP was being exploited for purposes beyond board test. In these early days, its utility was deployed to support access to chips for in-circuit emulation (debug), albeit often with additional pins for proprietary signals. Somewhat later, the ubiquity of the 1149.1 TAP was exploited in a normative sense for in-system configuration of programmable devices by way of IEEE Std 1532. Later still, use of the 1149.1 TAP as a debug interface was standardized by NEXUS 5001 (although still requiring additional signaling for many cases). Today, for the same reasons of utility and ubiquity, the 1149.1 TAP is considered the most likely means of access to chips that support embedded instruments per P1687 (informally known as Internal JTAG or IJTAG).Notwithstanding the exceptional merits of the 1149.1 TAP, ongoing industry momentum toward greater miniaturization and still more integration led some to the conclusion that a makeover was needed [1]. In particular, they proposed to enhance its functionality and utility in applications debug, but also to reduce pins to be better suited to multi-core/ multi-die architectures. IEEE Std 1149.7 [2, 3, 4, 5] has been developed to meet these needs [6, 7, 8, 9, 10, 11, 12].1.1What is IEEE 1149.7IEEE 1149.7 is a standard for a test access port and associated architecture that offers reduced pins and enhanced functionality. With regard to pin reduction, whereas the conventional 1149.1 TAP (TAP.1) requires at least four signals (with a fifth, for test reset, being optional), the reduced-pin 1149.7 TAP (TAP.7) requires only two signals (with the possibility for encoding the optional test reset function onto these). Further, with regard to functionality enhancement, it is expected that, in many cases, extended signaling needs for uses such as applications debug can be met on no more than two pins. Even while delivering these benefits, 1149.7 has taken great pains to preserve the investment that the industry has made in 1149.1 for chips and on boards. Particularly, 1149.7 adopts the entirety of the 1149.1 boundary-scan architecture to fully support board test and in-system configuration. Further, 1149.7 does not replace 1149.1, but rather adapts it and extends it, building upon its foundation and legacy. For example, as illustrated in Figure 1, an 1149.1 chip can be adapted easily to provide a TAP.7. As well, TAP.7s can coexist with TAP.1s on boards and, in some cases, even on the same board-level TAP connections.“before”“after”Figure 1—Adaptation of 1149.1 to 1149.71.2IEEE 1149.7 Key ObjectivesThe key objectives for 1149.7 fall broadly into three categories – system architecture, applications debug, and legacy infrastructure (to include test).Benefits for system architecture derive from the appropriate accommodation of multiple on-chip embedded TAP Controllers (EMTAPC), the reduction of pins, the adoption of glue-less star topology, independence from CPU/debug technology, and provisions for power management. Where intellectual property (IP) blocks may each contain EMTAPCs, multiples of these may be accommodated on a complex system-on-chip (SOC). While reduced pin count has inherent value with respect to miniaturization, consider as well that, in combination with the star topology, fewer pins better support the new 3D packaging methodologies such as system-in-package (SIP). These typically involve the stacking of die as shown in Figure 2; conversely, a daisy-chaining implementation is not only more difficult but also is not 1149.1 compliant. Of course, the same can be said for package-on-package (POP). Further, independence from particular CPU/debug technologies supports similar integrations even where chips may incorporate CPU IP from multiple sources. Facilities that permit the test logic to enter power-down modes support increasingly aggressive power management requirements.Figure 2—Star topology for a 3-die SIPFor applications debug, the TAP.7 provides advanced capability that reduces or eliminates the need for signaling beyond the two wires. Extensions provided include robust hot connect, increased throughput by way of scan optimization, higher operating frequency, and transport of background instrumentation data and/ or custom protocols. Of course, independence from CPU/debug technology also accrues here because uniform tool sets can support chips with heterogeneous CPUs.Finally, as concerns the legacy infrastructure, the objectives are two-fold – first, honor 1149.1 by preserving the test infrastructure that has been built up around it and on which the industry depends; and second, maintain a level of compliance such that existing intellectual property in chips, on boards, and in debug and test systems (DTS) can be adapted at low cost.2. Overview/ How it WorksAt the highest level of abstraction, 1149.7 provides for a chip-level TAP.7 Controller that bridges the conventional 1149.1-accessible System Test Logic (STL) to the four (five) or two (three) wires of the chip-level TAP.7 (the test reset signal is optional in either case). The STL has its own 1149.1 chip-level TAP Controller (CLTAPC) and all of the associated test logic architecture, including a chip-level boundary-scan register and related EXTEST, PRELOAD, and SAMPLE instructions.Seeking to extend 1149.1 in a compatible fashion, 1149.7 starts with the observation that the TAP Controller (TAPC) at the core of 1149.1 is a two-wire control means, even in the conventional series topology, as shown in Figure 3.TCK TDITDOTMSFigure 3—Conventional series topology, highlighting the starwiring for TCK/TMSPer 1149.1 convention, the starred TCK/TMS can only advance the TAPC state, which in turn invokes TDI/TDO for scan operations, but, absent instruction register scans, does not change the mode of the test logic. Thus, the key concept of 1149.7 continues with the observation that control extensions might be overlaid on sequences of TAPC states (more details on this later).Thus, 1149.7 adds its own commands and registers on which the other layers of extended functionality are based. These additional layers add scan formats, direct addressability, packetization of scan data (TMS, TDI, and/ or TDO information) onto the TMS wire (hence, re-designated TMSC), and finally packetization of non-scan information onto TMSC to provide for transport of background and/ or custom data.As such, 1149.7 supports a number of capability classes (six in number, designated as T0 - T5). So the TAP.7 Controller is configurable to support the required capability for a given implementation. The primary functional units are illustrated in Figure 4 and are designated as follows: Advanced Protocol Unit (APU), Extended Protocol Unit (EPU), Pin-Sharing Logic (PSL), and Reset and Selection Unit (RSU). The manner in which these items are invoked (or not) for given capability classes will be described in subsequent sections.TDI(C)nTRST EPU TAP.7TDO(C)TCK(C)TMS(C)TAP.7 ControllerFigure 4—Notional view of the 1149.7 architecture2.1 Capability classesRegardless of which capability class is implemented, a given TAP.7 must implement all of the mandatory features of its own class as well as those of all lower-numbered classes (T0 < T5). The classes are generally considered in two primary groupings: those which extend 4-wire operation (T0 – T3) and those which provide the reduced-pin, 2-wire operation (T4 – T5).T0–foundationAs the foundation of all TAP.7 capabilities, T0 begins with 1149.1-Specified behavior, such that the T0 STL is 100 percent compliant to 1149.1 including provisions for the mandatory chip-level boundary-scan architecture. With 1149.7 T0, the chip-level device identification register becomes mandatory.Of the TAP.7 Controller elements shown in Figure 4, only the RSU is permitted as an option; the other items are reserved for higher classes.Where the RSU is implemented, this would be done, as for any other class, to permit Escape Sequences and/ or Selection/Deselection Alerts to be available to manage the sharing of TAP.7 signaling across technologies and topologies. When the TAP.7 Adapter TAPC (ADTAPC) is deselected, the CLTAPC will be parked. These topics will be addressed in greater detail with T3 where the RSU becomes mandatory.Of particular note, even where the chip has multiple EMTAPCs, as might be the case for a complex SOC that implements a mix of several core IP blocks, the T0 STL shall provide 1149.1-Specified behavior from the Test-Logic-Reset TAPC state. 1149.7 identifies several means by which EMTAPCs can be selected under the control of the CLTAPC. A further method is defined for managing multiple die-level TAPCs in a similar manner for SIP. T1–commands and registersWith T1, the 1149.7 mechanism providing extended control by way of TCK/TMS is invoked to access 1149.7 commands and registers. These functions pertain to the EPU as illustrated in Figure 4. So, the EPU block of the TAP.7 Controller is present for T1. Otherwise, excepting the RSU, as for T0, all other blocks are reserved for higher classes.As described earlier, the extended control mechanism operates without disruption to the conventional 1149.1 TAPC state machine. Rather, it uses a particular state sequence, which is benign to normal 1149.1 operations, to initiate 1149.7-defined action.The state sequence of interest is known as a zero-bit DR scan (ZBS) and these are to be operated only while all of the CLTAPCs in the selected topology operate BYPASS or IDCODE so as to ensure that they do not disrupt normal system operation. In fact, ZBS detection is validated only when no IR scans have intervened since the last occurrence of the Test-Logic-Reset TAPC state.The progression of states that is recognized as a ZBS is illustrated in Figure 5.ScanCapture-DRExit1-DRExit2-DRUpdate-DRShift-DRPause-DR1110111abFigure 5—Zero-bit scan (ZBS)There are actually two different paths, labeled as “a” and “b” in Figure 5, that can implement a ZBS. In either case, the state sequence of interest is defined as follows: from the Select-DR-Scan TAPC state, proceed to the Update-DR TAPC state without passing the Shift-DR TAPC state. From the Test-Logic-Reset TAPC state, wherein the ZBS count is set to zero, the extended control mechanism is initiated when at least two ZBSs are detected before a subsequent non-zero-bit DR scan, which locks the ZBS count. A locked ZBS count of two provides access to the 1149.7 commands and registers. Locked ZBS counts greater than two access higher control levels that will not be detailed here.Once the ZBS count is locked, and a control level set, it is only unlocked when the control level is exited by entry to either the Select-IR-Scan or Test-Logic-Reset TAPC states or by certain 1149.7 commands and events that are used to synchronize T4 and T5 operations.At control level 2, commands are recognized, without the use of TDI/TDO pins, by counting the number of TCK(C)ticks in the Shift-DR TAPC state for two scans that immediately follow the completion of the non-zero-bit DR scan that locked the ZBS count. Each of these two primary command words is coded in 5 bits, ensuring that these counts need not exceed 31. All commands include two such parts for a total code length of 10 bits. In most cases, the command and register operations are concluded in these two parts but in some (only three) special cases a third part (a DR scan of a length determined by the control register addressed by the command) is required.T1 mandates a given minimum set of such commands and the registers that they address. Additionally, some commands, registers, and associated functions are optional, notably those that invoke directed test reset generation and request for functional reset.Additionally, T1 provides for power management through four modes of power control for the test logic. These four modes are: allow power down if TCK stops at logic one for more than 1 ms, allow power down if TCK stops at logic one for more than 1 ms in the Test-Logic-Reset TAPC state, allow power down if the device is in the Test-Logic-Reset TAPC state, and do not allow power down (the test logic is always powered).Given that a power-down mode is supported, the test logic is directed to resume powered operation when the Run-Test/Idle TAPC state is forced for at least 100 ms and at least 3 TCK(C) ticks.T2–scan formatsThe 1149.7 scan formats are introduced in T2. A T2 TAP.7 supports JScan0 – JScan2; other scan formats are reserved for higher classes.Of the TAP.7 Controller elements shown in Figure 4, for T2, as for T1, only the EPU is mandatory with the RSU permitted as an option; the other items are reserved for higher classes. For T2 versus T1, the EPU adds the commands and registers associated with the scan formats. As regards the function of these scan formats, JScan0 provides 1149.1-Specified behavior while JScan1 provides for the deselection of the CLTAPC in favor of a 1-bit scan path (so-called Super Bypass) that is active for IR scans as well as DR scans and JScan2 provides for activation/deactivation of the Super Bypass according to the value of an 1149.7 register.A T2 TAP.7 can opt either for JScan0 or JScan1 as its startup behavior. The latter case is described as providing hot-plug immunity since it should permit live connection (or disconnection) of the TAP.7 signals to be non-disruptive to the test logic.T3–direct addressabilityFinally, with T3, the star topology (4 wire), as in Figure 6, is supported. This comes by adding the means for direct addressability and the JScan3 scan format that provides for scans to star-4 topologies.Figure 6—Star-4 topologyOf the TAP.7 Controller elements shown in Figure 4, T3 adds the RSU as a mandatory element in addition to the EPU. For T3 versus T2, the EPU adds the commands and registers associated with direct addressability. Note that for T3, the TDI and TDO signals are re-designated as TDIC and TDOC, respectively.Concerning the RSU for T3, it is required to implement Escape Sequences for reset and for selection/ deselection and may optionally implement Selection and Deselection Alerts. Escape Sequences involve the detection and counting of a number of edges on TMS(C) driven while TCK(C) is held at logic 1. The count of such edges determines the action to be taken in response to the Escape Sequence. Alerts are specific predefined sequences of 128 bits as driven on TMS(C).The resource invoked for support of direct addressability is the TAP.7 Controller Address (TCA), as shown in Figure 7. The values corresponding to DEVICE_ID are inherited from the 1149.1 device identification register capture value for the CLTAPC. The assignment of the NODE_ID is made by some implementation-specific means that is not defined by 1149.7. The NODE_ID serves to distinguish multiple TAP.7s on a given topology branch where they are of the same device type.Figure 7—TAP.7 Controller Address (TCA)A key provision required to facilitate scans to the star-4 topology is the prevention of drive conflict on TDOC. The JScan3 format is managed so that when multiple TAP.7 Controllers are requested to participate, then drive on TDOC will be inhibited.As discussed in more detail in 4.1, test applications require the ability to coordinate the simultaneous entry of all devices of interest into and/ or through the Capture-xR, Update-xR, and Run-Test/Idle TAPC states. At first glance, the star topology would seem not to support this requirement. But in addition to the JScan3 scan format, T3 adds the Scan Selection Directives (SSD) to deal with this matter. The SSDs make use of Pause-xR TAPC states as parking states to which simultaneous scan captures aredirected and from which simultaneous scan updates are directed. Scan shift operations, as necessitated by the star topology are done on a device-by-device basis and are both directed from and to the Pause-xR TAPC states. T4–packetization of scan data (2-pin scan formats)With T4, a number of additional scan formats are added to support the advanced protocol, which is operated on two pins. The TCK/TMS pins are re-designated as TCKC/TMSC, respectively. The corresponding star-2 topology is illustrated in Figure 8. Note that TMSC is bidirectional.TMSCTCKCFigure 8—Star-2 topologyOf course, these additions require the provision of the APU of Figure 4. As well, since the TDIC/TDOC pins are optional since they are not used for 2-pin operation, where they are provided the PSL also becomes an option. Where a T4 TAP.7 does not provide the TDIC/TDOC pins in any configuration, it is described as narrow and designated as T4N. Where a T4 TAP.7 does have a configuration that provides the TDIC/TDOC pins, it is described as wide and designated as T4W.One of the more basic scan formats that supports the advanced protocol is OScan1. The serialization of the scan packet for OScan1 is illustrated in Figure 9. As shown, the TDI bit information is inverted. Also, for each cycle in which the TDO bit appears it is driven from the selected device in the target system back to the DTS.TCKCTMSC stateFigure 9—Scan packet serialization – OScan1Other scan formats in the OScan series provide for optimizations in which the scan packets omit bits that can be known to carry no significant information. An example worth noting is the OScan7 format which is optimized for downloads from the DTS to the target system. For OScan7, as illustrated in Figure 10, only the TDI bit information is included in the packets sent during Shift-xR TAPC states.TCKC nTDI nTDI nTDI nTDI nTDI nTDI nTDI nTDI nTDITMSC Shift-xRShift-xRShift-xRstateFigure 10—Scan packet serialization – OScan7Further performance optimization that can be obtained for T4 is by invoking falling-edge sampling for TMSC which, presuming hold times still can be met, delivers a degree of improvement in setup times that would allow the TCKC frequency to be increased (perhaps doubled).T5–transport of non-scan data (2-pin mode)At the top of the classes, T5 offers the capability to interleave transfers of non-scan data among the scan transfers. This is referred to as transport and has two variants – background data transport (BDX) and custom data transport (CDX).Both types of transport can use any combination of Run-Test/Idle, Pause-xR, and Update-xR TAPC states after which to insert transport packets. The distinction is that, whereas BDX has fixed allocation of I/O bandwidth available to the chip-level data channel, CDX has a custom allocation of I/O bandwidth as determined/ defined by the chip-level unit.2.2 Selection hierarchyWhile some aspects of the selection hierarchy have been described above, some additional detail is warranted as it is a key aspect of the 1149.7 system architecture.In general, where selection is enabled within the hierarchy, those items not selected are effectively offline/ parked and respond only to particular selection requests on the TAP.7 signaling. Those that are selected are online and respond to the TAP.7 signaling according to the protocols for which they are configured.Five levels in the selection hierarchy are elaborated below. For each level of the hierarchy, one or more selection mechanisms may pertain. - TechnologyWhere the 1149.7 technology can be placed offline, the TAP.7 signaling can be shared with other technologies- TopologyW here the constituent 1149.7 devices can be placed offline (a function required for T3 and above), the TAP.7 signaling can be shared among any topology branches, whether series, star-2, or star-4 - Adapter (i.e., ADTAPC)1149.7 devices comprising a selected topology branch will share TAP.7 signaling and, where the topology branch is star-2 or star-4, a given device may be selected for a given operation- Chip (i.e., CLTAPC)For a selected ADTAPC, the CLTAPC may be offline and will require selection when it must be operated- Core (i.e., EMTAPC)For a selected CLTAPC, given EMTAPC(s) of interest may be offline and will require selection when it (they) must be operated3.Implications for DebugIn many respects, 1149.7 was defined by and for the debug community. Thus, many of the implications, considerations, and supporting features have already been addressed in the elaboration of the basic 1149.7 functions as described above. Still some of these bear specific mention in this context.3.1Debug considerationsChief among the considerations for debug are ease and efficiency. Of course, these unfold across multiple dimensions and are often intertwined.EaseThe highest degree of visibility and control is required to drive the analysis tools that can get to the bottom of thorny problems that arise in today’s multi-threaded, multi-core, real-time embedded systems. 1149.7 promises to bring value to this equation by consolidating debug access for multiple cores onto a smaller number of chip pins.Other features tending toward ease of debug are the hot-plug immunity and system interrogation.EfficiencyCertainly 1149.7 enjoys a performance boost relative to 1149.1 with its various optimizations for scan throughput and its ability to improve link utilization by using otherwise idle non-scan states to transport background instrumentation data.3.2Debug featuresAs one would expect, 1149.7 brings a wealth of features to address debug ease and efficiency.Access consolidationSeveral aspects of the 1149.7 system architecture provide for access consolidation: management of EMTAPCs (T0), star topology (T3), pin reduction (T4N/T5N), and capability for the TAP.7 to transport background data and custom protocols (T5). All of these result in making debug instrumentation more accessible and, hence, easier to use. Hot-plug immunityLive connection without system disruption is vital and is enabled by the offline at startup (T3 or any with RSU) and Super Bypass at startup (T2) features. System interrogationA method for topology interrogation (T3) is provided and enumeration of controllers can be made by undirected allocation of Controller IDs (CID) (T3), as in Figure 11. These features allow the system architecture to be discovered by the debug tool at connect time.Each participating chip drives its AT[n]on wired-OR basis (logic 1 inactive)All supporting chips without CID participate;chips with a CID do not participate.For each participating chip, its aliasing targetAT[35:0] = TCA[34:0] + 0Final n?Each chip that detects that itsAT[n] != TDO drops outNext nThe chip that matched all 36 bitsof its AT to all 36 bits of TDOwins and gets the CIDFigure 11—CID Allocation, Undirected Optimization of scan throughputPerhaps above all, 1149.7 offers a great number of opportunities to optimize scan throughput. Super Bypass (T2) results in shorter scan chains for series topology. Star topology (T3) offers direct addressability and, hence, scan operation in only the target of interest.Advanced protocol (T4) offers still further improvements based on scan packet optimization on one hand and falling-edge timing (and thereby clock doubling) on the other.Improved link utilizationBDX and CDX (T5) allow the link to be used for transport of instrumentation data even during non-scan states, which would otherwise be idle.4.Implications for TestWhile 1149.7 primarily has been developed for the benefit of applications debug, it also has gone to considerable lengths to ensure that the test legacy of the original 1149.1 is honored. Among the several test-related provisions are the scan selection directives that ensure update/ capture/ run-test synchronization and the definition of suitable test languages.4.1Test considerationsWhile 1149.7 does not change radically how 1149.1 boundary scan/ test is being used today, there are some new considerations for test that arise with the 1149.7 architecture. Primary among these are the divergence inscan-state sequencing and series/star interoperability. Other considerations worth mention are hierarchical navigation, power control, and large-system applications. Scan-state sequencingThe typical test application has requirements for scan-state sequencing that necessitate coordination of application of stimuli across multiple distinct components (with their own boundary-scan registers) on a given unit under test (UUT). Consider the case where several components have distinct 3-state outputs on a shared wire junction. If the distinct output control cells in each of these components were not updated in a coordinated fashion, a contention could be present on the shared wire. The simplest means of such coordination is to ensure that all components pass simultaneously through the Update-DR TAPC state. As well, while it might not be necessary in some cases, the coordination of the acquisition of response generally also is required (or at least preferred). At the least, where specific response to given stimuli is required, the distinct responses for each component involved must all be acquired before the applied stimulus is changed. Here again, the simplest means for such is to ensure that all components pass simultaneously through the Capture-DR TAPC state (in fact, simultaneity may be strictly required in some special cases).Some test applications, such as the testing of advanced digital networks per IEEE Std 1149.6, have an additional requirement that all components participating in such testing must pass simultaneously through the Run-Test/Idle TAPC state.Conventional scan sequencing for test applications presuming series topology is illustrated in Figure 12.Run-Test-Idle1Select-DR-ScanCapture-DRExit1-DRExit2-DRUpdate-DRShift-DRPause-DR110111Figure 12—Scan-state sequence (conventional) for testapplications, series topologyThis conventional scan-state sequence can be broken down as follows:- (in yellow) all components on the UUT (series topology) pass through the Capture-DR TAPC state wherein responses (to a previously applied stimulus) are acquired in concert- (in red) all components on the UUT (series topology) enter and remain in the Shift-DR TAPC state until all responses have been exported and all new stimuli have been imported- (in blue) all components on the UUT (series topology) pass through the Update-DR TAPC state wherein new stimuli are applied in concert- (in grey) all components on the UUT (series topology) dwell in/ pass through the Run-Test/Idle TAPC state wherein transient stimuli may be generated in concert The divergence introduced by the 1149.7 star topology is that, due to shared wiring of TDIC and TDOC for star-4 or TMSC for star-2, only one component may be operated in the Shift-DR TAPC state at a time.Fortunately, we can take from the above discussion that the Shift-DR TAPC state is actually neutral to the requirements of the test application. Thus, the needs of the test application can be served, even for a star topology if each of the TAPC states Capture-DR, Update-DR, and Run-Test/Idle can be operated in concert across all of the UUT components of interest.It is for just this purpose that the scan selection directives (SSD) were devised. Figure 13 illustrates the scan-state sequence made possible by the SSDs (star topology).Run-Test-Idle1Select-DR-ScanCapture-DRExit1-DRExit2-DRUpdate-DRShift-DRPause-DR11111Figure 13—Scan-state sequence for test applications,modified for star topology。

开启片剂完整性的窗户(中英文对照)

开启片剂完整性的窗户(中英文对照)

开启片剂完整性的窗户日本东芝公司,剑桥大学摘要:由日本东芝公司和剑桥大学合作成立的公司向《医药技术》解释了FDA支持的技术如何在不损坏片剂的情况下测定其完整性。

太赫脉冲成像的一个应用是检查肠溶制剂的完整性,以确保它们在到达肠溶之前不会溶解。

关键词:片剂完整性,太赫脉冲成像。

能够检测片剂的结构完整性和化学成分而无需将它们打碎的一种技术,已经通过了概念验证阶段,正在进行法规申请。

由英国私募Teraview公司研发并且以太赫光(介于无线电波和光波之间)为基础。

该成像技术为配方研发和质量控制中的湿溶出试验提供了一个更好的选择。

该技术还可以缩短新产品的研发时间,并且根据厂商的情况,随时间推移甚至可能发展成为一个用于制药生产线的实时片剂检测系统。

TPI技术通过发射太赫射线绘制出片剂和涂层厚度的三维差异图谱,在有结构或化学变化时太赫射线被反射回。

反射脉冲的时间延迟累加成该片剂的三维图像。

该系统使用太赫发射极,采用一个机器臂捡起片剂并且使其通过太赫光束,用一个扫描仪收集反射光并且建成三维图像(见图)。

技术研发太赫技术发源于二十世纪九十年代中期13本东芝公司位于英国的东芝欧洲研究中心,该中心与剑桥大学的物理学系有着密切的联系。

日本东芝公司当时正在研究新一代的半导体,研究的副产品是发现了这些半导体实际上是太赫光非常好的发射源和检测器。

二十世纪九十年代后期,日本东芝公司授权研究小组寻求该技术可能的应用,包括成像和化学传感光谱学,并与葛兰素史克和辉瑞以及其它公司建立了关系,以探讨其在制药业的应用。

虽然早期的结果表明该技术有前景,但日本东芝公司却不愿深入研究下去,原因是此应用与日本东芝公司在消费电子行业的任何业务兴趣都没有交叉。

这一决定的结果是研究中心的首席执行官DonArnone和剑桥桥大学物理学系的教授Michael Pepper先生于2001年成立了Teraview公司一作为研究中心的子公司。

TPI imaga 2000是第一个商品化太赫成像系统,该系统经优化用于成品片剂及其核心完整性和性能的无破坏检测。

Edexcel BTEC Level 2和3信息技术系统和原则通信技术单元指南说明书

Edexcel BTEC Level 2和3信息技术系统和原则通信技术单元指南说明书

Unit 29: Communication TechnologiesUnit code: F/601/7264QCF Level 3: BTEC SpecialistCredit value: 10Guided learning hours: 60Aim and purposeThe aim of this unit is to ensure learners understand the communication principles of computer networks, know the elements of data communications systems and develop the skills to implement network communications.Unit introductionNew and developing communication technologies are used within the business world to maximise productivity and access information, whether an employee is working an office or travelling the world.This unit focuses on communication technologies, examining their use within social and business communities. Learners will explore the devices and communication technologies they use on a daily basis and gain an understanding of systems, including mobile internet. Learners will look at network topologies and services, connection software and access methods amongst other areas. They will investigate the OSI and TCP/IP models. Communications technology includes a large range of devices, which are used in the business and social communities. Devices include many of the next generation wireless devices, games consoles and newer generations of mobile phones with voice and video streaming. This unit explores these devices along with their transmission methods and the underlying protocols that enable connectivity and transmission of data including signal theory and data elements. Learners will appreciate why particular transmission methods are chosen for particular situations and be able to compare the effectiveness of the different methods.Finally, learners will be able to use their knowledge and understanding to directly connect communication devices between users.This unit could be delivered as part of a system support and networking focus including other units with networking content. Although largely theoretical, the emphasis in this unit should be on practical activity to learners to understand how modern IT can be used to enhance the commercial and personal environment. This unit has links directly to other network units and the vendor units.Learning outcomes and assessment criteriaIn order to pass this unit, the evidence that the learner presents for assessment needs to demonstrate that they can meet all the learning outcomes for the unit. The assessment criteria determine the standard required to achieve the unit.On completion of this unit a learner should:Learning outcomes Assessment criteria1 Understand thecommunication principlesof computer networks 1.1 explain how networks communicate1.2 identify communication protocols and models2 Know the main elementsof data communicationssystems 2.1 identify different types of communication devices 2.2 describe what data elements are and why theyare important2.3 describe the principles of signal theory2.4 describe different transmission methods used3 Be able to implementdifferent forms of networkcommunications 3.1 create direct network communication betweentwo users3.2 set up interconnection devices for directcommunicationUnit content1 Understand the communication principles of computer networksComputer networks: types eg LAN, WAN, wireless; network topologies eg star; mesh;bus; tree (or hierarchical); ring; network services eg packet switched, ISDN,multiplexed, ATM, WAP, broadband; network software eg network operating system;network connection software; access methods eg CSMA/CD, CSMA/CA, token passing Network components: servers; workstation; network cards eg Ethernet, wireless, token ringInterconnection devices: eg switches, routers, bridges, wireless access points, mobile base stationsModels: eg open system interconnection (OSI) model, TCP/IP modelProtocols: eg Bluetooth®, Wifi, IrDa, cellular radio, GSM/UMTS, WAP, WML, 802.11 standards, TCP/IP, wireless security protocols2 Know the main elements of data communications systemsMain elements: communication devices; data elements; electronic communication methods; transmission media and methodsCommunication devices: wired devices eg data terminal equipment (DTE), data circuit terminating equipment (DCE); wireless devices eg 3G, GPRS, mobile phone, laptop, netbookSignal theory: digital signalling methods; representing data electronically (bits, bytes, packet structures); synchronous transmission; asynchronous transmission; error detection; error correction; bandwidth limitation; bandwidth noise; channel types eg telephone, high frequency (HF) radio, microwave, satellite; other issues egbandwidth, data compressionData elements: checksum eg cyclic redundancy check (CRC); encapsulation egframes, packets, datagrams; addresses; sequence numbersElectronic communication: methods eg simplex, duplex, half-duplex communication, parallel, universal serial bus, serial, infra red, Bluetooth, WiFi, 3GTransmission: methods eg coaxial, optical fibre, unshielded twisted pair (UTP),shielded twisted pair (STP), infrared, radio, microwave, satellite3 Be able to implement different forms of network communicationsInternet communication: terminology eg HTTP, HTTPS, FTP, SMTP; uniform resource locator; worldwide web; other eg blogs, wikis, video conferencing, vlogs, socialnetworkingSystem requirements: wired or mobile systems; communication services eg email, video, internet, software, configurationDirect communication: eg instant messaging, video communication, email, webphone, social networking, web conferencing, desktop sharingInterconnection devices: eg switches, routers, bridges, wireless access points, mobile base stationsEssential guidance for tutorsDeliveryThis unit could be delivered as part of a system support and networking focus which includes other units with networking content. The emphasis is on practical activity to enable learners the to understand how modern IT can be used to enhance the commercial and personal environment. This unit links directly to other network units and the practical element in 3.1 and 3.2 could link with the vendor units.Delivery of the theoretical aspects should focus on the most up-to-date technology available, including all the latest developments in mobile communications such as third generation technology.The practical aspects of the unit that are practical should be opportunities to use the main technologies listed in the unit content, such as 3G hardware and mobile computing technology, for example, a wireless PDA. Ideally, the tutor should try to secure as many of these technologies as is feasible. For those that the centre cannot provide, it is recommended that the tutor tries and sources outside examples of this form of technology to demonstrate to learners.As an addition to the practical element, it may be useful for learners to have some input from an IT communications technology specialist, who can give an insight into career progression within this sector, as well as providing up to the minute technical knowledge that learners can draw on.Outline learning planThe outline learning plan has been included in this unit as guidance and can be used in conjunction with the programme of suggested assignments. The outline learning plan demonstrates one way in planning the delivery and assessment of this unit.Topic and suggested assignments/activities and/assessmentIntroduction to the unitThe main elements of data communications systems:•whole-class exercise – tutor presentation on communication devices•directed research – using tutor-provided materials on signal theory•whole-class exercise – tutor presentation on data elements•whole-class exercise – tutor presentation on methods of electronic communication, followed by whole class•practical•whole-class exercise – tutor presentation on transmission media and methods, followed by whole class•practical.Communication principles of computer networks:•individual exercise – use tutor-provided materials to understand the features of networks•whole-class exercise – tutor demonstrates network components to class•whole-class exercise – tutor demonstrates interconnection devices to class. Assignment 1 – From End to EndTransmission protocols and models:•whole-class exercise – tutor presentation on the open system interconnection (OSI) model and the TCP/IP•model, followed by a practical•individual exercise – learn about different electronic communication protocols from tutor-provided materials.Assignment 2 – A Bespoke SolutionImplementing different forms of internet communications:•whole-class exercise – tutor presentation on internet communication terminology, followed by practical tasks•whole-class exercise – tutor presentation on system requirements, followed by practical tasks•whole-class exercise – tutor presentation on direct communication methods, followed by practical tasks.Assignment 3 – LAN ManagerAssessmentThis unit can be assessed using three assignments as outlined in the Programme of suggested assignment table. However, there are other valid ways of assessment and the programme of suggested assignment is only a suggestion.Suggested Assignment 1 – From End to EndEvidence for this assignment could be in the form of a series of leaflets, posters or web pages which are suitably illustrated and annotated.1.1 requires learners to explain how networks communicate. This can be evidenced diagrammatically with annotations and notes, or it could be part of a presentation.For 1.2, communication protocols and models should be described using graphics where appropriate.For 2.1, learners must identify different types of communication devices, addressing the prescriptive elements of the unit content.For 2.2, learners must describe what data elements are and why they are important. Learners need to describe the different elements, and their importance, in their own words.Suggested Assignment 2 – A Bespoke SolutionA presentation is the suggested evidence format for this assignment.2.3 requires learners to describe the principles of signal theory as outlined in the unit. 2.4 is about transmission methods.Suggested Assignment 3 – LAN Manager3.1 is practical, and each learner must create a direct communication link between two networked devices. This could be a short video, observation evidence, or a written summary.3.2 is also practical. In this case, learners must create interconnection devices for direct communication. As with 3.1, evidence could be a short video, observation evidence, or a written summary.Programme of suggested assignmentsThe table below shows a programme of suggested assignments that cover the assessment criteria in the assessment and grading grid. This is for guidance and it is recommended that centres either write their own assignments or adapt any Edexcel assignments to meet local needs and resources.Criteria covered Assignment title Scenario Assessment method1.1-2.2 From End toEnd An ISP hascommissioned you towrite publicity materialsexplaining some of thebasics of ITcommunication.Leaflets or poster.2.3, 2.4 A BespokeSolution A client wishes to knowmore abouttransmission signals.Produce a presentationto describe theprinciples and methodsused.Presentation.3.1, 3.2 LAN Manager The ISP has asked youto create a system thatwill enable two users tomake directcommunication. Observation records. Witness statement. Notes.Links to National Occupational Standards, other BTEC units, other BTEC qualifications and other relevant units and qualificationsThis unit forms part of the BTEC in IT sector suite. This unit has particular links with: Level 1 Level 2 Level 3Systems ArchitectureArchitectureSystemsSetting up an IT Network Networking PrinciplesNetworking Principles Computer NetworksAn Introduction toCommunicationTechnologiesThis unit maps to some of the underpinning knowledge from the following areas of competence in the Level 3 National Occupational Standards for IT (ProCom):4.7 Systems Design5.1 Systems Development5.3 IT/Technology Solution Testing.Essential resourcesLearners will need access to practical resources and suitable technology; they can also use simulators or multimedia tools to gain prior experience before handling live resources.Employer engagement and vocational contextsVisits to a local ISP or using the centre’s network would provide a suitable vocational context.Indicative reading for learnersTextbooksDodd AZ – The Essential Guide to Telecommunications, 4th edition (Prentice Hall, 2005) ISBN-10 0131487256, ISBN-13 978-0131487253Hallberg B – Networking: A Beginner’s Guide, 5th Edition (Osborne/McGraw-Hill US, 2009) ISBN-10 0071633553, ISBN-13 978-0071633550Lowe D – Networking All-in-One Desk Reference for Dummies, 3rd Edition (John Wiley & Sons, 2008) ISBN-10 0470179155, ISBN-13 978-0470179154Schiller J – Mobile communications, 2nd Edition (Addison Wesley, 2003) ISBN-10 0321123816, ISBN-13 978-0321123817WebsitesFunctional Skills — Level 2Skill When learners are …ICT – Using ICTcreating network communicationsSelect, interact with and use ICTsystems safely and securely fora complex task in non-routineand unfamiliar contextsICT – Developing, presenting andcommunicating informationsetting up communication devicesUse communications software tomeet requirements of a complextaskexplaining protocols and transmission methods. Combine and presentinformation in ways that are fitfor purpose and audience。

Building and Environment

Building and Environment

逄秀锋,等:我国建筑调适发展现状与前景指南与标准、建立激励机制以及政策法规、走向市场化产业化。

我国建筑调适的发展目前也遵循了这样一条发展路径,不同的是,我们的目标是用更短的时间完成西方国家四十多年走过的道路。

参考文献:[1]Mills E.Commissioning Capturing the Potential[J].ASHRAE Journal,2011,53(2):1-2.[2]逄秀锋,刘珊,曹勇.建筑设备与系统调适[M].北京:中国建筑工业出版社,2015:1-2.[3]Legris C,Choiniere D,Milesi Ferretti.Annex47Report1:Commissioning Overview[R].Paris:International Energy Agency,2010.[4]The U.S.Department of Energy.New DOE Research Strengthens Business Case for Building Commissioning[EB/OL].(2019-05-02)[2020-01-02].https://www.energy.gov/eere/buildings/articles/ new-doe-research-strengthens-business-case-building-commissioning.作者简介:逄秀锋(1976),男,辽宁人,毕业于美国内布拉斯加大学林肯分校,暖通空调专业,博士,研究员,研究方向:建筑调适技术、建筑系统能耗模拟、暖通空调系统故障诊断与优化控制、智慧建筑(xpang113@163.com)。

Energy and Buildingshttps://www.sciencedirect.com/journal/energy-and-buildings/vol/224/suppl/CVolume224,1October2020(1)A new analytical model for short-time analysis of energypiles and its application,by Jian Lan,Fei Lei,Pingfang Hu,Na Zhu,Article110221Abstract:An energy pile is a special form of vertical ground heatexchanger that couples the roles of structural support and heat trans-fer.Modeling the transient heat transfer process inside an energy pilehas importance;however,available analytical models either have in-sufficient calculation accuracy or are computationally demanding.Based on three existing models,this paper proposes a novel short-term hybrid composite-medium line-source(HCMLS)model,whichis not only efficient in computation but also more accurate than mosttraditional analytical models.The model is suitable for ground heatexchangers of various radii.Comparisons between the hybrid analyti-cal model and a numerical model are made for energy pile cases withdifferent parameters,including the thermal properties,borehole radii,relative positions of tubes,and number of tubes.In general,the hy-brid composite-medium line-source model gives credible predictionafter100min.The new model is further validated by the infinitecomposite-medium line-source(ICMLS)model,which is currentlythe most theoretically complete short-term model.Moreover,the newmodel is applied to thermal response tests(TRTs).The least dimen-sionless test duration for interpretations based on the modified hybridcomposite-medium line-source(C-HCMLS)solution is Fo>1.7.This study renders the application of in situ TRTs to energy pileswith large diameters feasible.Keywords:Ground heat exchanger;Energy pile;Short time re-sponse;Thermal response testing(2)Charging performance of latent thermal energy storage sys-tem with microencapsulated phase-change material for domestichot water,by Y.Fang,Z.G.Qu,J.F.Zhang,H.T.Xu,G.L.Qi,Arti-cle110237(3)Thermographic2D U-value map for quantifying thermalbridges in building fa ades,by Blanca Tejedor,Eva Barreira,Ricardo M.S.F.Almeida,Miquel Casals,Article110176(4)Urban morphology and building heating energy con-sumption:Evidence from Harbin,a severe cold region city,by Hong Leng,Xi Chen,Yanhong Ma,Nyuk Hien Wong,Tingzhen Ming,Article110143(5)UK Passivhaus and the energy performance gap,by Ra-chel Mitchell,Sukumar Natarajan,Article110240Building and Environmenthttps://www.sciencedirect.com/journal/building-and-environ-ment/vol/183/suppl/CVolume183,October2020(1)Residential buildings airtightness frameworks:A reviewon the main databases and setups in Europe and NorthAmerica,by Irene Poza-Casado,Vitor E.M.Cardoso,Ricar-do M.S.F.Almeida,et al,Article107221Abstract:The airtightness of buildings has gained relevance in thelast decade.The spread of the regulatory frameworks,the demand ofstricter requirements,schemes for testing and quality control,the cre-ation of airtightness databases and its analysis,is proof of this real-ity.The present review encompasses schemes developed in Europeand North America with regard to these aspects for national residen-tial sectors.A normative framework on requirements and recommen-dations at the national level is compiled.Whole building airtightnessdatabases are compared based on their structures and measurementdata acquisition protocols.Gathered complementary information notdirectly related to testing is analysed and airtightness influencing fac-tors importance and relationships are discussed.Weaknesses andstrengths in the different aspects of the existing database setups areidentified.Also,neglected or not entirely undertaken topics are pin-pointed together with the suggestion of possible opportunities forfuture works and changes.Amongst other relevant remarks and dis-cussions,it is concluded that the lack of uniformization in methodbetween countries,the need for a minimum data setup,the lack ofdata analysis on relating the energy impact with the advancement inrequirements of airtightness performance and the implemented setupsare some of the main issues to address in the near future.Keywords:Review paper;Airtightness;Regulation policy(2)A simulation framework for predicting occupant thermalsensation in perimeter zones of buildings considering directsolar radiation and ankle draft,by Shengbo Zhang,Jamie P.Fine,Marianne F.Touchie,William O’Brien,Article107096(3)Comparative review of occupant-related energy aspectsof the National Building Code of Canada,by Ahmed Abdeen,William O’Brien,Burak Gunay,Guy Newsham,HeatherKnudsen,Article107136Applied Energyhttps://www.sciencedirect.com/journal/applied-energy/vol/275/suppl/CVolume275,1October2020(1)Performance characteristics of variable conductance loopthermosyphon for energy-efficient building thermal control,byJingyu Cao,Xiaoqiang Hong,Zhanying Zheng,et al,Article115337Abstract:Variable conductance loop thermosyphon(VCLT)manip-ulates natural phase-change cycle to regulate the heat transfer.Its pri-mary advantages include high sustainability,simple design and lowcost.One of the potential applications of variable conductance loopthermosyphon is thermal control in buildings for achieving highenergy efficiency.In this study,a distributed steady-state model wasimplemented to determine the heat transfer control characteristics ofvariable conductance loop thermosyphon for the first time and evalu-ate its effectiveness on precise air-conditioning for buildings.The in-ternal flow resistance rises from0.002K/W to0.305K/W and theheat transfer rate decreases from468.5W to71.9W when the rela-tive opening degree of the regulating valve reduces from1.00to0.17under normal boundary conditions.The thermodynamic analysesshow that the regulating valve of the variable conductance loop ther-mosyphon can enable effective thermal control over a wide range ofheat transfer rate to accomplish indoor thermal comfort.The studyalso reveals that variable conductance loop thermosyphon can be ef-fectively adopted with various working fluids and over wide rangesof heat source and heat sink temperatures.Keywords:Air-conditioning;Energy-efficient building;Loop ther-mosyphon;Numerical study(2)Increasing the energy flexibility of existing district heatingnetworks through flow rate variations,by Jacopo Vivian,Dav-ide Quaggiotto,Angelo Zarrella,Article115411(3)A framework for uncertainty quantification in buildingheat demand simulations using reduced-order grey-box en-ergy models,by Mohammad Haris Shamsi,Usman Ali,EleniMangina,James O’Donnell,Article115141(2020-10-10《建筑节能》杂志社侯恩哲摘录)7。

First-principles study of the structural, vibrational, phonon and thermodynamic

First-principles study of the structural, vibrational, phonon and thermodynamic

1. Introduction Ultra-high temperature ceramics (UHTCs) with melting temperatures in excess of 3000 K are usually composed by the refractory borides, carbides and nitrides of early transition metals [1–7]. Among the UHTCs, transition metal carbides (TMC) such as TiC, ZrC and HfC are metallic compounds with unique physical and chemical properties including an extremely high melting point and hardness, chemical stability, corrosion resistance combined with metallic electrical and thermal conductivities [5–10]. These features give transition metal carbides the capability to withstand high temperatures in oxidizing environments, making them candidates for applications in the atmosphere of extreme thermal and chemical environments [6,7]. The structural, vibrational, phonon and thermodynamic properties of IVb group transition metal carbides have been investigated experimentally [10–17] and theoretically [13,18–28] in the earlier reports. In the 1970s, the phonon dispersion relations of TiC, ZrC and HfC were measured using inelastic neutron scattering by Pintschovius et al. [10] and Smith et al. [15–17]. Lattice dynamics calculation and the phonon dispersion relations of transition metal carbides such as ZrC and HfC were reported using a phenomenological ‘‘double-shell’’ model theory [18] where long-range interatomic interactions were taken into account in order to get a

手术患者的交接流程与手术安全核查流程

手术患者的交接流程与手术安全核查流程

英文回答:The handover process for surgical patients is a pivotal aspect of ensuring patient safety and maintaining continuity of care. Effective transfer of a patient from one clinical area to another, such as from the pre-operative area to the operating room, necessitates a meticulous handover process. This involves the utilization of standardized tools and checklists to guide the handover process, coupled with precise and unambiguousmunication amongst members of the healthcare team. The handover should epass aprehensive verbal exchange of crucial patient information,plemented by meticulous documentation in the patient's medical record to forestall oversight of important details. Adherence to a structured and standardized handover process serves to minimize the risks of errors, omissions, and mimunication, ultimately contributing to enhanced patient safety and improved oues.手术病人的移交过程是确保病人安全和维持护理连续性的一个关键方面。

AusMed_Brings_Game-Changing_Healthcare_Innovations

AusMed_Brings_Game-Changing_Healthcare_Innovations

23Quick and accurate non-invasive ketone level measurements Ketones are versatile biomarkers for both DKA and ketosis management. DK A is a serious complication of diabetes mellitus, and can rapidly deteriorate and become life-threatening if not treated in time. DKA may result from the low availability of insulin which could reduce the efficiency of cells to take in and turn blood glucose into energy. Meanwhile, ketosis is a natural metabolic process of fat in which the body produces ketone bodies from stored fat as an alternative source of energy when there is not enough glucose. It has been revealed that there are many potential benefits of ketosis, including weight loss, protection of brain function, reduced frequency of seizures, killing of some cancer cells, reduction of inf lammation, control over non-alcoholic fatty liver disease, accelerated post-exercise recovery, and “A s a non-invasive alternative to the traditional finger-prick blood ketone testing devices, boasting a high level of accuracy comparable to industrial gold standards typically attainable only through bulky and expensive specialty equipment, we proudly introduce the world’s first-of-its-kind handheld breath ketone (BrAce) analyser, empowered by our patented disruptive photonic sensing technologies. These innovations are aimed at providing a more effective approach to managing diabetes and ketosis (fat metabolism),” said Christine Yip, founder and CEO of AusMed Global Limited, during a recent interview with China’s Foreign T rade magazine.Headquartered in the Hong Kong Science and Technology Park, AusMed is a fast-growing life science company. In the six years from 2017 (when the non-invasive sensing technologies for diabetes monitoring first took shape) to 2023 (when the final prototype was by Guo Yan AusMed Brings Game–Changing Healthcare Innovations from Hong Kong to the Worldcompleted and tested), AusMed has been progressing solidly and steadily under the lead of Christine and is now preparing to take the leap to the next stage I. It is preparing to launch its first model of the KetoMetrics breath ketone analyzer (KetoMetrics ) later this year. AusMed has obtained ISO 9001 (GB/T 19001) and ISO 13485 (YY/T 0287) certifications and has registered as an FDA medical device establishment. Amongst the many achievements, AusMed won the silver medal at the 2022 Geneva International Exhibition of Inventions for its fat loss management solution. Christine commented that success in commercializing a new technology does not happen overnight and is a result of the joint effort and long-term commitments of the R&D teams striving for innovation excellence and their passion for a better world, and the steadfast support of investors and collaborators who share the same vision.24improvements in sports performance.At present, the most common way to monitor ketone levels is through a finger-prick test, but this approach is painful and increases the risk of wound infection. AusMed’s novel handheld high-performance breath ketone analyzer is a game changer in the field of ketone measurement. It has significant advantages over the conventional metal-oxide-based breath meters in the market.“In contrast to conventional metal-oxide-based meters, our analyzer employs a patented disruptive photonic sensing technology that is highly selective to BrAce but no other volatile organic compounds such as ethanol and methanol. Therefore, the existence of evaporated alcohol gas in a hospital environment or even the consumption of alcohol will not affect the accuracy of the measurement. In addition, since breath ketone is an irreversible final product of fat metabolism, measuring ketones to monitor ketosis can help learn how quickly the body burns fat, so as to develop the most effective ketosis and weight management plan for peoples’ daily demands.” Yip said.This novel KetoMetrics needs only a breath sample to provide a measurement wit h a n acc u rac y equ iv a lent toindustrial gold standards for VOC gas measurement (i.e., Gas Chromatographywith Flame Ionization Detection(GC-FID) R2>0.99) within a minuteanywhere and anytime, making itsuitable for both clinical and home uses.In addition, AusMed’s KetoMetricsis deployed across a wide span of medical and healthcare applicationsfor breath ketone measurements, including DKA screening, nutritionalketosis, ketosis for improvements insports performance, and therapeuticketosis. In some cases, DKA may occurwhen the blood glucose level remainsrelatively normal (i.e., euglycemicdiabetic ketoacidosis (EDKA)), andthis may conceal the underlying DKAunder conventional screening protocols,posing diagnostic challenges. AusMed’sportable BrAce analyser provides atimely, non-invasive method for breathketone detection, regardless of bloodglucose levels, which can mitigate therisks for both patients and healthcareproviders due to a delay in the discoveryof the EDKA condition.KetoMetricshousehold packs will be provided with washable bottles, and disposablebreath sample bottles will be provided for devices used in hospitals, clinics, and other public organizations, in order to make sure each breath ketone measurement is clean and safe.Last but not least, KetoMetricsadopts a one-kit one-test design like the blood and urine ketone testing limitingthe carryover contamination issue, which is very critical in medical and healthcare applications. Various challenges were overcome during the development process from concept to soft launch “Initially we aimed to help children with Type 1 diabetes to measure their ketone levels in a non-invasive way for effective DKA management with scientif ic achievements. In 2017, when we discovered that the concept of accurately measuring breath ketones in the form of acetone with patented breakthrough photonic sensing technology originally from the University of Sydney, Australia to be feasible, and we began to study the possibility of turning the proven concept into a real product. However, it took us more than 5 years from choosing feasible technology to the soft launch of a product, and we encountered more difficulties than expected,” Yip said emotionally.During the process of development, the team encountered one problem after another. How could they shrink the size of the analyzer, while integrating more than 100 electronic parts intoKetoMetrics household packs will be provided with washable bottles, and disposable breath sample bottles will be provided for devices used in hospitals, clinics, and other public organizations, in order to make sure each breath ketone measurement is clean and safe.25existing products ? How would a heavy or soft breath affect the accuracy of the measurements and the smoothness of the airflow in the ketone sensors ? Would these sensors integrated into the analyzer produce water vapor and how would the vapor affect the measurements ? After measurements, where should the results be stored for a long time, so as to generate health reports ?“Research and development were accelerated after a lab was set up in Hong Kong Science and Technology Park in 2019. Since the five years’ of R&D which cost over RMB 40 million, the product took shape in 2021. Since then, we have obtained patent registrations and approvals in China, the U.S., and Australia. The final prototype of KetoMetrics was completed in mid-2022, and the soft launch of the first model will be in late 2023.” Yip said.It was discovered that the first-generation KetoMetrics has completed prototype testing and is now in the clinical studies phase at Queen Mary Hospital and Hong Kong Science and Technology Park. According to Yip, moving forward, AusMed will continue its clinical cooperation with other universities, health science clinicians, researchers, and local private healthcare providers, so as actively to participate in the global diabetes and healthcare communities, to study the ways to improve the health management of athletes and other users using ketone data, and to collect feedback suggestions in developing non-invasive solutions under other pipelines. KetoMetrics and AI big data analytics In order to allow for a better understanding and use of measurement data anytime and anywhere, KetoMetrics can be connected, via Bluetooth, to AusMed’s novel KetoMetrics AI diet management system and app, so users can capture and log their breath ketone measurement results, daily diet, and personal health data from wearable devices, in order to generate personal health reports for dietary management and support their health plans. U sers can also share their personal reports with their healthcare professionals and personal trainers for individual hea lthcare counseling. The new KetoMetrics AI diet management system and app promote healthy lifestyles, coinciding with the emerging international trend that smart devices should support telemedicine and primary healthcare initiatives.“We developed algorithms that can calculate the readings from one breath sample to derive ketone measurement results, and clarify the relationship between ketone levels and diet, lifestyle, stress, and so on with big data analysis, so as to identify any potential underlying health issues, in order to provide suggestions for the users to improve their fat loss plans. In this way, users can burn fat, while keeping their nutrition balanced,” Yip said.“With KetoMetrics being available, we aim to empower diabetes communities and healthcare professionals with a non-invasive and highly selective tool for monitoring ketone levels, thereby optimizing support and care for those in need.” Nowadays, data security has become a growing concern. Regarding the safe use of monitoring data in the future, Yip said, “we attach great importance to data privacy and security. Backend data protocols will not sendany data to his/her doctors, dietitians,or anyone else without being authorized by the user. All data will be handled properly in accordance with local laws and regulations and will not be shared across regions.”Taking business opportunities in the Greater Bay AreaAs for the reason that AusMed is headquartered in Hong Kong, Yip explained that Hong Kong is an international financial center with a simple tax regime, a robust legal system and a vibrant capital market, and there are state-of-the-art R&D facilities, a well-established supply chain network, and a regional hub of family offices, private equity funds and venture capital. Besides this, Hong Kong is close to mainland China.Since moving into Hong Kong Science and Technology Park in 2019, AusMed has received a great amount of support, from setting up laboratories, forming new research teams, and applying for ITC funding from the HKSAR Government. AusMed has also received great support from the Hong Kong Productivity Council, Invest Hong Kong, and the Hong Kong Trade and Development Council for global outreach.Yip highlighted that, in the future, AusMed plans to explore both the business-to-business (B2B) and business-to-consumer (B2C) markets. As ketones are versatile biomarkers for both DKA and ketosis management, the target marketsegments for KetoMetrics include diabetescommunities, pregnant women, weightmanagement and sports communities, aswell as members of the public looking forwellness and a healthy lifestyle.“AusMed Global will focus onHong Kong, Southeast Asia, mainlandChina, the Middle East and the UnitedStates over the next two years. There arechallenges in penetrating different marketsas regulatory approval processes vary acrossmarkets. The most effective way is to finda local partner to sort out the regulatoryapproval requirements and procurementsystems of healthcare providers in differentplaces,” continued Yip.As for new financing plans in thefuture, Yip said, “Recently, we have beenin talks with some family offices, PEfunds and CVC regarding investmentopportunities. Some distributors andinsurance companies also contactedus and talked about opportunities forcooperation. We are open to differentoptions and look forward to engagingwith strategic partners and cornerstoneinvestors for our long-term growth, andto our pursuit of an IPO in Hong Kong.”Focusing on the cultivation ofyoung generation’s creativityIn recent years, despite AusMed’shighly demanding work, Yip has alsodisplayed a strong commitment tothe development of young talent. Asthe world’s first youth forum in thefield, and modeled after internationalconferences, the CAFEA Young TalentSmart City Forum, curated by Yip, wasfirst held in 2021. This annual eventaims to establish a platform for youngpeople from Hong Kong, Macau, themainland, and beyond to cultivateinnovative ideas, share knowledge,and foster friendships among schoolstudents. Its overarching mission is toignite a profound interest in science,technology, innovation, and the creationof future cities among the young, and ithas already achieved notable success.Yip said, “Young people arethe future of society, and we need tostimulate their interest in innovativetechnology, scientific research, andsmart digital cities. The forum is toinspire creativity in the youth andencourage them to practice what theylearn, in order to build a smart campus,a smart city, and ultimately an efficientand comfortable smart city cluster in theGreater Bay Area”.It was reported that a total of46 school teams from Hong Kong,Shenzhen, and Macau were shortlistedfor the 3rd CAFEA forum held in July2023, and they discussed how to developthe Greater Bay Area into a world-classsmart city cluster. Chief executive ofthe Hong Kong Special AdministrativeRegion, John Li Ka-Chiu, said in hiscongratulatory message that the forumis of great significance as it provides avaluable interactive platform for studentsfrom Hong Kong, Macau, and Shenzhen,not only deepening their understandingof a smart city but also promoting theirfriendship and the exchange of ideasregarding the development strategies forthe Greater Bay Area.The 3rd CAFEA forum was heldafter the three years of the COVID-19pandemic, and the participants were ableto gather in Hong Kong for the first time.Yip explained, “It is a rare opportunity.I hope that, through the forum, youngpeople can go beyond classroom learningand make greater social contributions.They are students today, but tomorrow’sdesigners and builders of the smartcity cluster in the Greater Bay Area. Infuture, we will invite more students fromschools in China and other countries andregions to the forum and make it a trulyinternational event”.26。

PYMS小儿约克希尔营养不良评分信息和用户指南说明书

PYMS小儿约克希尔营养不良评分信息和用户指南说明书

PYMS Paediatric Yorkhill Malnutrition ScoreInformation and User’s Guide2009© Nutrition Tool Steering Group, Women and Children’s Directorate, NHS Greater Glasgow and Clyde, 2009.The content of this booklet is provided for general information only and should not be relied upon. Whilst we use all reasonable efforts to ensure that the information contained in the booklet is current, accurate and complete at the date of publication, no representations or warranties are made (express or implied) as to the reliability, accuracy or completeness of such information. Greater Glasgow Health Board cannot therefore be held liable for any loss arising directly or indirectly from the use of, or any action taken in reliance on, any information appearing in the booklet.The information in this booklet is designed to comply with the laws and regulations of the United Kingdom. Although accessible by users from other countries, it and its content is intended for access and use by residents of the United Kingdom only. Disclaimer:- ALL RIGHTS RESERVED. No part of this publication may be copied, modified, reproduced, stored in a retrieval system or transmitted in any material form or by any means (whether electronic, mechanical, photocopying, recording or otherwise and whether or not incidentally to some other use of this publication) without the prior written permission of the copyright owner except in accordance with the provisions of the Copyright, Designs and Patents Act 1988.ContentsPage Introduction (3)Development of the Paediatric Yorkhill Malnutrition Score (PYMS) (5)Validation of the Paediatric Yorkhill Malnutrition Score (PYMS) (5)PYMS User’s Guide (6)The PYMS Form Explained (13)PYMS Form (15)Nurse’s Quick Reference Guide (17)Quick Guides for Measuring Heights/Lengths and Weights (19)References (23)“Freedom from…malnutrition is a basic human right”(World Health Organisation, 20081).IntroductionThe Paediatric Yorkhill Malnutrition Score (PYMS) has been developed to assist nursing staff and other health professionals identify hospitalised children, between the ages of 1-16 years, who are at risk of malnutrition and offer them appropriate care. The following explanatory notes offer an overview of malnutrition and provide information on the development and use of PYMS.MalnutritionIdentification of children at risk of malnutrition is essential in treating disease-related malnutrition and optimising the health of all hospitalised patients. Malnutrition is: “the state of nutrition in which a deficiency or excess (or imbalance) of energy, protein, and other nutrients causes measurable adverse effects on tissue/body function and clinical outcome”2. The term malnutrition refers to both over-nutrition and under-nutrition but, for the purposes of this document it only refers to energy/protein under-nutrition.Malnutrition continues to be a significant health issue in developed countries, with an estimated cost in the UK of approximately £13 billion3. Despite this malnutrition continues to be largely unrecognised and under treated4. It has been estimated that up to 30%5-10 of hospitalised patients are at risk of malnutrition and this figure can be as high as 60% in some paediatric patient groups11,12.Why is Malnutrition Important?Malnutrition is undesirable, not only because it leads to weight loss, but also because it is a recognised risk factor for the development of complications of disease. These include increased morbidity and mortality, longer duration of hospitalisation and increased health care costs13. In children, there are additional concerns as malnutrition can potentially lead to long-term effects on brain development, linear growth and bone health that impact on health later in life14.Failure to consider nutritional status may also have medico-legal consequences, with an increasing number of cases of nutritional neglect being pursued within the judicial system15.In the general population approximately 2.5% of children have a body weight which is below the reference range for their age and gender. However, a much larger proportion (16%) of children attending hospital are underweight and malnutrition remains largely undiagnosed and untreated amongst hospitalised children9-10. This is mainly due to a lack of nutritional training and awareness amongst staff and can also be attributed to a lack of established protocols for screening, assessment and action15.Introduction to Nutritional ScreeningFood and water are essential elements of care and failure to detect malnutrition or the risk of becoming malnourished has the potential to cause patients considerable harm. The Nursing and Midwifery Council (NMC) Code of Conduct requires all nursing staff to ‘protect and promote the health and well being of those their care’16. This responsibility is detailed in NHS Quality Improvement Scotland Guidelines (2003) which state that because high quality nutritional care is crucial for the well being of patients, all patients should be screened using a validated tool that is appropriate to the patient population17. Such screening should be carried out on admission and regularly during a patient’s hospital stay. Nutritional screening provides a means of ensuring that patients, who following screening appear to be at high risk, will be assessed by dietetic staff and managed appropriately.Screening tools validated in adult patients18-19 areinappropriate for use in children, as malnutrition presentsdifferently within the paediatric population. Thus far,nutritional screening in paediatrics has been hindered due toa lack of a valid generic paediatric screening tool15.Development of the Paediatric Yorkhill Malnutrition Score (PYMS)A multidisciplinary healthcare team from the Royal Hospital for Sick Children, Glasgow and Royal Alexandra Hospital, Paisley, both part of the Women and Children’s Directorate NHS Greater Glasgow and Clyde, was assigned to develop a local paediatric malnutrition screening tool. The project team consisted of senior nursing, dietetic, research, academic and medical staff.The primary purpose of the project team was to develop and validate a tool that would be simple, quick to use, user and patient friendly and would detect the majority of children at risk of malnutrition. The tool would be used by nursing staff to screen patients on admission and at intervals during their hospital stay. The tool was developed for use in children aged 1-16 years. A separate tool was considered necessary to assess neonates and infants under the age of 1 year, due to their rapid growth during the first year of life and complex issues surrounding prematurity.A preliminary malnutrition screening tool was developed based on the guidelines for nutritional screening from the European Society of Clinical Nutrition and Metabolism15. The PYMS was designed to incorporate questions/measurements to address the following four principles:1. The current nutritional status2. The stability of nutritional status3. The recent changes to nutritional status4. The likelihood of the acute disease condition to affect the nutritional statusadversely.Validation of the Paediatric Yorkhill Malnutrition Score (PYMS)Following the development of the tool a 4 month validation study was undertaken, within 4 paediatric wards (3 medical, 1 surgical) of a tertiary paediatric hospital and the general paediatric ward of a district general hospital. The diagnostic accuracy and performance of PYMS was evaluated through a four stage validation study, the results of which have been presented20-22 and are expected to be published in due course.PYMS User’s GuideDescription of the PYMS FormThe PYMS form is presented as a simple structured questionnaire, consisting of four questions (steps) which are strong predictors/symptoms of malnutrition. Each of these steps bears a score from 0 to 2 and an overall nutritional risk score (step 5) is calculated based on the sum of the results of steps 1-4. An action plan follows according to the overall nutrition score. The four steps are outlined below:Step 1: Body Mass Index (BMI)•BMI is a useful measure of nutritional risk and is based on height and weight.•Height and weight should be obtained according to local hospital guidelines.•Weight must be repeated each time PYMS is carried out and it is recommended that for long term admissions, height/length should be recorded monthly for infants and three-monthly for older children.(N.B. please follow local hospital policy).Instructions outlining the correct procedure for obtaining weights and heights/lengths are included in this information & users guide (page 21-24) (N.B. please follow local hospital guidelines). Once measurements have been correctly obtained, they should be recorded in the appropriate boxes on the PYMS form. After that, the Body Mass Index (BMI) for the patient can be calculated using a BMI calculation wheel.The BMI calculation wheel consists of two wheels (see figure 1).To use:•Locate the weight (kilograms) of the patient on the outer wheel and the height/length (centimetres) of the patient on the inner wheel.•Rotate the inner wheel until child’s weight and height are aligned.•The BMI value is displayed in the window by the red arrow, record the value obtained in the appropriate box on the PYMS form.Figure 1: BMI Calculation Wheel(Produced with permission from Blundell Harling Ltd.)Normal BMI values vary according to age and gender and therefore a scoring guide located on the back of the PYMS form, gives the minimum acceptable BMI values. The age of the child should not be rounded up when referring to this scoring guide. A BMI below the minimum acceptable value indicates a possible risk factor for malnutrition. The following scoring should be used for step 1:i. Score 0 if BMI value is greater than that shown for age and gender according to the scoring guide.ii. Score 2 if BMI value is below that shown for the age and gender according to the scoring guide.NB : If it is NOT POSSIBLE TO OBTAIN A HEIGHT, a member of medical staff should be asked to plot the patient’s weight on a growth chart. If the weight is below the 2nd centile, a SCORE of 2 should be entered for step 1.The reason why a height could not be obtained should be documented in the comments table on the back of the form.STEP 2: Recent Weight LossUnintentional weight loss may indicate that a child is at nutritional risk. Ask parents/guardians if they have noticed any recent weight loss, or compare current weight with previously documented weights. These should be recorded in the patient’s notes or parent hand held record.If the child is under 2 years of age, parents/guardians should be asked if they have any concerns about the child’s weight gain recently. Failure to gain weight may also be an indication of nutritional risk in very young children (<2 years).i. Score 0 if:a. Weight is increasingb. Weight is static and child is more than 2 years oldc. Weight loss is intentional as the child has been or is overweight andis on a calorie restricted diet.ii. Score 1 if:a. Unintentional weight loss noticed by the child/carers or has beenidentified after comparing with previously documented weightsb. Weight static in a child less than 2 years oldc. Clothes have become more ill fitting due to noted weight lossd. Intentional weight loss because of eating disorderse.g. if the childsuffers from anorexia nervosa.STEP 3: Assess Recent Change in Diet/Nutritional Support (for at least the last week)A decreased nutritional intake may increase the risk of developing malnutrition. Ask carers/child about food intake for at least the last week. If the child is usually on any artificial feeds (enteral feeds, dietary supplements or parenteral nutrition) then ask whether there has been any change in the amount taken and/or tolerated.i. Score 0 if:a. There has been no change to normal diet or enteralnutrition, dietary supplements or parenteral nutritionii. Score 1 if:a. There has been a decrease in usual dietary intake, enteralfeeds, dietary supplements or parenteral nutrition for a minimum of thelast 7 days (unless health professional instructed decrease to restrictcalorie intake).iii. Score 2 if:a. There has been no or minimal intake over the last week, includingintake from oral feeds, enteral nutrition, dietary supplements orparenteral nutrition.N.B. If there is very minimal intake (only a few sips of feed per day) this should be counted as no intake (score 2).STEP 4: Acute Admission/Condition Effect on Nutrition (at least the next week) Some patients may be at risk of becoming undernourished during their hospital admission or soon after their discharge, because of the effect of the medical condition on their nutritional status. This may be due to decreased intake, increased gut losses and increased energy requirements.i. Score 0 if:a. The patient’s nutritional status is unlikely to be unchanged duringthis admission or soon after their discharge.ii. Score 1 if at least one or more of the following is expected over at least the next week:a. Decreased intake from oral, enteral or parenteral nutrition(e.g. orofacial disease or trauma, severe nausea)b. Increased gut losses (e.g. significant ongoing diarrhoea or vomiting,large stoma losses)c. Increased energy requirements (e.g. major trauma, burns, sepsis,pyrexia).iii. Score 2 if for the next week:a. No or minimal intake is expected from oral, enteral orparenteral nutrition (e.g. major abdominal surgery).N.B. If there is very minimal intake (only a few sips of feed per day) this should be counted as no intake (score 2).STEP 5: Total Nutrition Risk Score and Action PlanOnce all the above scores have been entered onto the PYMS form add them together to provide a total score (step 5). A total score of 2 or more reflects significant nutritional risk.i. If the score is 2 or more, a request for dietetic review should bemade according to the established hospital request system andthe medical/surgical team should also be informed. PYMSshould be repeated weekly.ii. If the score is 1, then the child should be observed for any further deterioration of intake. The quantity and type of food andfluid consumed should be recorded. PYMS should be repeatedin 3 days and medical staff informed.iii. If the score is 0, then no further action is required at this time but PYMS should be repeated weekly.N.B.If there are clinical concerns about a patient’s nutritional status, a dietetic review MUST be requested and medical staff informed even if the child has scored less than 2.Local policies and clinical judgement are not replaced by this scoring system and children should be managed following local guidelines for nutrition management. This should include plotting of height and weight on a growth chart and any additional nutritional concerns discussed with the medical/surgical team and a request made to dietetics if deemed necessary (e.g. food allergies, special diets etc).PYMS is designed to detect children at risk of energy/protein under-nutrition (malnutrition) only, and will therefore not detect children with vitamin and/or mineral deficiencies. It is not designed to detect children at risk of over-nutrition (obesity).The PYMS Form ExplainedLocal hospital policy may require that 2 peoplecheck weight and height measurementsObtain each time PYMS is calculatedRecord calculated BMI here- obtain using BMI wheel.Age required to calculate BMI and compare against cut off valueMeasure length/ height monthly in infants and 3 monthly in older childrene.g; • Major trauma• Major burns • Sepsis • Pyrexiae.g;• Persistent diarrhoea • Persistent vomitinge.g;• orofacial disease/ trauma • severe nauseaUnless a health professional hasinstructed reduction in calorie intake.Use BMIscoring guide overleafStanding Height MeasurementFeet straight together with soles touching the boardBare feet, legs togetherHeels, calves, buttocks, shoulder, head touching theback plateReference List1. World Health Organisation (WHO) (2008) Nutrition for health and development. [online] http://www.who.int/nutrition/nhd/en/index.html Accessed 28th April 2008.2. Elia M, Ljungqvist O and Dowsett J (2005) Principal of Clinical Nutrition: Contrasting the Practice of Nutrition in Health and Disease. Oxford, Blackwell Science Ltd.3. Bapen (2009) Combating Malnutrition Recommendations for Action. Executive summary. Redditch, British Association of Parenteral and Enteral Nutrition (BAPEN) [online] /pdfs/reports/advisory_group_report.pdf Accessed 15th July 2009.4. McWhirter JP and Pennington CR (1994) Incidence and recognition of malnutrition in hospital. British Medical Journal 308 945-948.5. Hendricks KM, Duggan C, Gallacher L, Carlin AC, Richardson DS, Collier SB, Simpson W and Lo C (1995) Malnutrition in hospitalized paediatric patients: current prevalence. Archives of Pediatrics and Adolescent Medicine 149 (10) 1118-1122.6. Joosten KFM, Zwart H, Hop WC and Hulst JM (2009) National malnutrition screening days in hospitalized children in the Netherlands. Archives of Diseases in Childhood[published online 3 May 2009] doi:10.1136/adc.2008.157255.7. Edington J, Boorman J, Durrant ER, Perkins A, Giffin CV, James R, Thomson JM, Oldroyd JC, Smith JC, Torrance AD, Blackshaw V, Green S, Hill CJ, Berry C, McKenzie C, Vicca N, Ward JE and Coles SJ (2000) Prevalence of malnutrition on admission to four hospitals in England. The Malnutrition Prevalence Group. Clinical Nutrition 19 191-195.8. Moy RJD, Smallman S and Booth IW (1990) Malnutrition in a UK children’s hospital. Journal of Human Nutrition and Dietetics 9 93-100.9. Pawelleck I, Dokoupil K and Koletzko B. (2008) Prevalence of malnutrition in paediatric hospital patients. Clinical Nutrition 27 72-76.10. Hendriske WH, Reilly JJ and Weaver LT (1997) Malnutrition in a children’s hospital. Clinical Nutrition 16 13-18.11. Cameron JW, Rosenthal A and Olson AD (1995) Malnutrition in hospitalised children with congenital heart disease. Archives of Pediatrics and Adolescent Medicine 149 (10) 1098-1102.12. De Staebel O (2000) Malnutrition in Belgian children with congenital heart disease on admission to hospital. Journal of Clinical Nursing 9 (5) 784-791.13. Correia MI and Waitzberg DL. (2003) The impact of malnutrition on morbidity, mortality, length of hospital stay and costs evaluated through a multivariate model analysis. Clinical Nutrition 22 235-9.14. Lucas A, Morley R and Cole TJ (1998) Randomised trial of early diet in preterm babies and later intelligence quotient. British Medical Journal 317 1481-1487.15. Kondrupt J, Allison SP, Elia M, Vellas B and Plauth M. (2003) ESPEN Guidelines for Nutrition Screening 2002. Clinical Nutrition 22 415-421.16. Nursing and Midwifery Council (NMC) (2008) The Code. Standards of Conduct, Performance and Ethics for Nurses and Midwives. London, Nursing and Midwifery Council.17. NHS Quality Improvement Scotland (QIS) (2003) Food, fluid and nutritional care in hospitals. Edinburgh, NHS QIS.18. Gerasimidis K, Drongitis P, Murray, L, Young D and McKee R (2007) A local nutritional screening tool compared to malnutrition universal screening tool. European Journal of Clinical Nutrition 61 916-921.19. Green SM and Watson R (2005) Nutritional screening and assessment tools for use by nurses: literature review. Journal of Advanced Nursing 50 (1) 69-83.20. Gerasimidis K, Macleod I, McGrogan P, Maclean A, Buchanan E, McAuley M, Stewart G, Wright C and Flynn D (2009) Development and Performance of a New Paediatric Nutritional Screening Tool in a Tertiary and District General Hospital. The PYMS Project. British Society of Paediatric Gastroenterology, Hepatology and Nutrition (BSPGHAN) winter meeting (Sheffield), oral presentation.21. Gerasimidis K, Keane O, Macleod I, Buchanan E, Maclean A, McGrogan P, Stewart G, McAuley M, Flynn D and Wright C (2009) Criterion validity and inter-rater reliability of the Paediatric Yorkhill Malnutrition Score. European Society of Paediatic Gastroenterology, Hepatology and Nutrition (ESPGHAN) annual meeting (Budapest), oral presentation, abstract in press.22. Macleod I, Gerasimidis K, Purcell O, Mohammed T, Swinbank I, Wright C, FlynnD and McAuley M (2009) Implementing a novel paediatric nutritional screening tool (Paediatirc Yorkhill Malnutrition Score) in nursing practice.Challenges and impact in paediatric nursing practice. European Society of Paediatic Gastroenterology, Hepatology and Nutrition (ESPGHAN) annual meeting (Budapest), poster presentation, abstract in press.NotesProduced by The Nutrition Tool Steering Group,Women and Children’s Directorate,NHS Greater Glasgow and Clyde.Medical Illustration, Job No. 20584727。

我的理想是当一名飞行员英语作文

我的理想是当一名飞行员英语作文

我的理想是当一名飞行员英语作文篇1My Dream is to Be a PilotHi there! My name is Timmy and I'm 10 years old. Today I want to tell you all about my huge dream of becoming a pilot when I grow up. Flying up in the clouds seems like the most amazing job in the whole wide world!Ever since I was a tiny little kid, I've been obsessed with airplanes. When I was a baby, my parents say I would get all excited and start kicking my little feet whenever a plane flew overhead. I guess I was just born to fly!My very first plane ride was when I was 4 years old. We went on a family vacation to Florida to visit my grandparents. I still remember sitting by the window, staring out in total awe as the houses and cars got smaller and smaller until everything looked like a miniature toy town below.As we climbed higher into the sky, I could see the crazy patterns that the roads make and all the different shades of blue in the lakes and oceans. It was like getting a bird's eye view of thewhole world! That plane ride sparked my lifelong fascination with flying.From that day on, every time I went past an airport, I would plead with my parents to stop so I could watch the planes take off and land. I would run back and forth on the viewing deck, keeping count of how many I saw. I knew all the different aircraft models just from their silhouettes and the sounds of their engines.For my 6th birthday, my parents took me to an air museum where I got to actually go inside the cockpit of an old fighter jet. You can't imagine how enormously excited I was! I sat there playing with all the knobs and buttons, making sound effects like "neeeeeeoooowwww" and pretending I was the pilot. That's when I decided – this was going to be my future career.At home, I'm always building new model airplanes to hang from the ceiling of my bedroom. I have models of all the classics – a Spitfire, a Concorde, the Spirit of St. Louis, you name it. But my number one favorite is the space shuttle. I really want to be an astronaut pilot one day and travel way up into outer space!In school, my best subjects are science and math because I know pilots need to understand all that stuff about aerodynamics, navigation, geometry, physics and more. I'm notjust into the technical aspects though – I also read every book I can find about famous aviators and their daring adventures.My personal hero is Amelia Earhart, the first woman to fly solo across the Atlantic Ocean back in 1932. She was such a trailblazer and showed that girls can soar just as high as the boys if we work hard and follow our dreams. I have a big poster of her plane hanging above my bed to inspire me.Another awesome aviator I admire is Bessie Coleman, who was the first African American woman to get a pilot's license back in 1921 when they definitely did not make it easy for women or Black people to enter that career. Her motto was "Because of Dreamers of Dreams" which I think is just beautiful.So what exactly is it that makes me want to be a pilot so darn badly? Well, first of all, can you imagine how incredible the views must be up there? Getting to soar amongst the clouds every single day, watching the sun rise and set over endless landscapes down below. Just picturing it gives me goosebumps!But it's not just about the scenery. I think the real thrill of being a pilot is the actual feeling of flight itself. Roaring down the runway, pulling back on the controls and breaking free of the earth's gravitational pull until you're cruising free as a birdthousands of feet up in that vast open sky. It would be a brand new adventure every single day.Of course, being a pilot is about way more than just having fun. It's a massively important job with huge responsibilities to get passengers and cargo safely to their destinations on time. Those people's lives are literally in your hands when you're at the controls. So you always have to stay focused and alert, ready to make split-second decisions if anything goes wrong mid-flight. I may be just a kid now, but I promise I'll have the discipline it takes.When you think about it, pilots basically get to travel the world for a living. How cool is that?! After high school, my plan is to attend an elite aviator university to get all the highest-level training and licenses I'll need. Then I hope to fly jumbo jets for a major international airline so I can visit all my dream destinations – Tokyo, Sydney, Rio de Janeiro, Cairo, you name it!I can picture myself now in my crisp pilot's uniform, shaking hands with my crew, going through all the pre-flight checklists and doing my safety briefing for the passengers. "Welcome aboard Flight 293 with service from New York to London. We'll be cruising at an altitude of 35,000 feet..." I'll scan the horizon,get my clearance from air traffic control and then rev up those powerful engines to take us up, up and away!Maybe I'll even try for a career as a fighter pilot in the Air Force one day. That would be the ultimate thrill ride! Imagine me at the stick of a supersonic jet, performing daring aerial maneuvers and rocket-fast banking turns, spiraling and rolling and looping through the sky. I'd love the chance to serve my country while pursuing my passion up among the clouds.Of course, being a pilot is not all fun and games. It's one of the most challenging careers out there that takes intense study, keen mental focus and split-second reaction skills. There's a ton of information pilots need to master about aerodynamics, weather patterns, air traffic control procedures, emergency protocols and so much more. But I'm ready to put in the incredibly hard work it will take because this is my absolute life dream.Becoming a pilot will open up a whole new world for me that most people only glimpse in passing out an airplane window. I'll get to experience the freedom of flight, explore exotic destinations, take on life-or-death responsibilities, and soar high above the clouds day after day after day. To me, that sounds a million times better than being stuck behind a desk.So that's my huge, ambitious, sky's-the-limit dream for my future career! Every time a plane engines roars overhead, I'll look up at that stream of smoke with determination, knowing that one day I'll be the one up there, bravely navigating us through the wild blue yonder. The clouds are calling me. I was born to fly!篇2My Dream is to Become a PilotEver since I was a tiny kid, I've always loved looking up at the big blue sky and watching airplanes soar through the clouds. Whenever I see an airplane cruising high above, I get super excited and start daydreaming about what it would be like to be the pilot flying that amazing machine!Being a pilot is the best job in the whole world, in my opinion. It seems so thrilling and adventurous. I love the idea of traveling to lots of cool places and getting to see the whole world from up in the air. How awesome would it be to look down at different countries and cities as you fly over them? I'd get to gaze at famous landmarks and natural wonders from thousands of feet up. That's a view that hardly anyone ever gets to experience.I also think the actual task of piloting an aircraft looks really fun and challenging. All those controls and buttons in thecockpit, it reminds me of the controls on my video games! Except being a pilot is a million times cooler because you're actually controlling this gigantic airplane. I can't even imagine how exciting it would be to be in charge of taking off, flying through the air, and landing a huge passenger jet or cargo plane. It seems so difficult, but I'm positive I could learn all the skills with hard work and practice.The best part is, you get to wear that really awesome pilot uniform with the hat, sunglasses, and everything. I'd look so stylish and professional marching through the airport in that killer outfit. When I'm a pilot, I'll take lots of selfies in the cockpit and make all my friends jealous of my super cool job. They'll all wish they could trade places with me and travel around in airplanes all day!I know becoming a pilot involves years of intense training and studying to learn all about aviation and get certified. But I don't mind working incredibly hard because this is my ultimate dream career. I'm going to stay focused in school, especially on subjects like math, science, and geography that will help prepare me. Then after high school, I'll go to an amazing aviation university or join the Air Force to receive top-notch flight instruction.Of course, there could be some downsides too. Like having to be away from home constantly while flying to different places. And being cramped in a tiny cockpit for hours on really long flights. Oh, and dealing with rude, grumpy passengers! I'm not looking forward to that part. But no job is perfect, so I'll take the good with the bad gladly.Another concern is the danger aspect. Flying an airplane seems very risky, one small mistake and terrible things could happen. But modern aircraft are incredibly safe with so many backups and security measures in place. As long as I'm a responsible, alert pilot who follows all the procedures, I'll be fine. I'm not a reckless daredevil at all. When I'm in that cockpit, you can be sure I'll be taking the utmost care and caution.So in conclusion, my number one dream for the future is to have an exciting, rewarding career as a professional pilot. Just picturing myself at the controls of a powerful aircraft, soaring majestically above the clouds, makes me grin from ear to ear. It's been my biggest aspiration since before I can even remember. With hard work and determination to overcome the challenges, I'm confident I have what it takes to make this dream a reality. Maybe one day I'll even be a famous pilot who gets interviewedon TV! But either way, I'll be living out the adventure of a lifetime up in the skies.篇3My Dream of Being a PilotEver since I was a tiny kid, I've wanted to be a pilot when I grow up. When I close my eyes, I can imagine myself up there in the clouds, flying a huge airplane all over the world! How cool would that be?I think pilots have one of the most exciting jobs ever. They get to travel to amazing places instead of being stuck in an office all day. Every time they go to work, it's like taking a vacation to somewhere new and fascinating. One day they could be in Paris, the next in Tokyo! They get to experience so many different cultures and cuisines. Just the thought of it makes me hungry for sushi or a warm, flakey croissant.But even more than the travel, I dream about the actual flying part. Pilots are like birds, soaring up in the wide open sky. They get an incredible view that hardly anyone else can see - a tiny world down below with roads like little lines and cars looking like ants. Up in the clouds, everything seems so peaceful and free. No traffic jams or crowds to deal with up there!Thinking about all the controls in the cockpit makes me giddy too. There are so many buttons, switches and screens to operate. It's like being at the controls of a real-life video game or spacecraft! Except in this game, hundreds of lives are in your hands. That's an amazing responsibility that I wouldn't take lightly. But I'm a good student who follows directions closely, so I know I could handle it.I love watching planes take off and land when my family goes on trips. As we pull away from the gate, I press my face against the window and thrill at the power of the engines roaring to life. Taking off is like getting shot from a cannon - except instead of tumbling back to earth, the plane climbs higher and higher until the whole scene down below is a wonderful quilt of colors. Even after years of flying, I don't think I'd ever get used to or tired of that adrenaline rush.Landing is like a magic trick where you defy gravity itself. The plane is this huge heavy machine that somehow floats back down to earth so smoothly and gently. It's a good thing pilots have lots of great training, because landing seems much trickier than taking off to me. I would study very hard in flight school to learn those specialized skills.There are so many other amazing parts of being a pilot that I can't wait to experience one day. Like talking to the control tower and getting clearances for takeoff and landing. Or flying through different kinds of weather like wind, rain, clouds or sun. And learning all about the complicated inner workings of an airplane's engines and systems. I'm usually more of anarts-and-crafts kind of kid, but for aircraft stuff I'll make an exception and study every single detail of mechanics and engineering.When I'm a pilot, I'll get to wear one of those awesome uniforms with the crisp white shirt, epaulets on the shoulders, and pair of wings on my chest. I'll look so official and important! Plus you get to carry around a fancy briefcase with your name and flight charts inside. I bet that gets you great service and all the little extras whenever you go out to restaurants or hotels between flights.I know becoming a pilot involves many years of school, flight training, and experience before anyone will trust me with a real aircraft. But I'm patient and determined. I'll start early by taking free intro flying lessons at a local airport as soon as I'm old enough. Then I'll join the aviation club at school, get greatgrades in math and science, and apply for scholarships to college and flight school.My parents are being super supportive of my dream so far. Sometimes on weekends, Dad takes me down to the nearby regional airport to watch the small charter planes take off and land up close. We pack a picnic lunch and spend hours just taking in that beautiful noise of the engines and the unmistakable aviation smells of jet fuel and hot runways. It never gets old!Last year for my birthday, I got an awesome flight simulator video game and some books about aircraft design and famous pilots throughout history. I've already read them cover to cover at least three times! The game is so realistic with all the same controls and procedures that real pilots use. Dad says if I keep practicing on it, I'll get a head start on my training once I begin actual flying lessons.The only thing I'm a tiny bit nervous about is having to move around so much for my job. Most pilots don't get to live in one place for too long before getting reassigned to a different airport or route. I'll miss my friends and extended family whenever we move. But then again, how amazing will it be to make new best friends all over the world? They'll be jealous that I get to travelfor a living while they're stuck at home or in the same boring town forever.I've had this pilot dream for longer than I can even remember. To me, there's no better way to live than up in the skies, far above the earth, with a new amazing view out my window every day. The thought of it is so freeing and magical. Some kids want to be astronauts, some want to be pro athletes or movie stars when they grow up. But for me, my future is in the cockpit of an airliner - comfortable, focused, in control, and soaring off to parts unknown while others can only look up from below and wonder what incredible sights I'm seeing. I can't wait to make my dream reality and spend my career with my head literally in the clouds!。

写海底世界我多想去看看的英语作文

写海底世界我多想去看看的英语作文

写海底世界我多想去看看的英语作文全文共3篇示例,供读者参考篇1The Underwater World, How I Wish to See ItEver since I was a little kid, I've been fascinated by the marine world. Whenever we'd go to the beach, I could spend hours exploring the shallow waters near the shore, turning over rocks and chasing tiny fish and crabs. The idea of venturing deeper beneath the surface has always captivated my imagination. What wondrous sights await in the depths of the ocean?I remember the first time I saw a vibrant coral reef in a nature documentary. A whole universe of life unfolded before my eyes - a kaleidoscope of colors and strange, alien shapes. Coral structures of brilliant yellows, purples and greens formed twisting towers and canyons on the ocean floor. Schools of tropical fish in every shade of the rainbow darted through the branches like living gemstones.Moray eels with grotesque, gaping jaws peered out from rocky crevices. Camouflaged stonefish and scorpionfish lay perfectly still, their venomous spines raised menacingly. ANapoleon wrasse the size of a small child cruised by imperiously. That single glimpse opened my eyes to the magic and dynamism teeming in the ocean depths.I've devoured every book, magazine and video about marine biology and underwater exploration I could get my hands on over the years. Yet for all the breathtaking imagery, there's no substitute for the real thing. I yearn to experience that world firsthand - to descend into that foreign realm and immerse myself in its mysteries.I dream of diving through a warm tropical lagoon,fin-kicking over pristine coral gardens as clouds of angelfish and butterflyfish scatter around me. Of coming face-to-face with the curious gaze of a large green sea turtle gracefully propelling itself through the water. Of watching an octopus's incredible camouflage ability in action as it shifts color and texture to blend seamlessly with its surroundings before slipping away.Some of the most astounding underwater vistas, though, are found in the cold, nutrient-rich waters of the temperate and polar regions. There, vibrant softies, sea whips and coral trees sway languidly with the currents, their branches hosting entire ecosystems of peculiar critters. Grotesque wolf eels withprotruding fangs and muppet-like red lips lurk under rocky overhangs.Translucent ctenophores, or comb jellies, drift past like ghostly apparitions, their iridescent bodies pulsating with rainbow hues. Perhaps I'll even spot species so recently discovered they remain unnamed and little-studied. The largely unexplored ocean depths hold so many unseen wonders still waiting to be uncovered.While it would be incredible enough just seeing these types of underwater landscapes, my ultimate dream is to go one step further and become a part of them, if only temporarily. To pull on a specialty Antarctic drysuit and bi-bubble mask and experience the chill of the Southern Ocean as I hunker down and observe life clinging to the underside of the permanent ice shelves.Or to submerge myself beneath the Arctic pack ice, equalizing to the heart-stoppingly frigid 28°F temperature s to witness the seasonal migration of bowhead whales, narwhals and belugas passing overhead. They are just two examples of the unique and seldom-seen environments I hope to one day immerse myself in.Beyond the diving itself, I'm equally fascinated by the human ingenuity and technology that make exploring the ocean depthspossible. The very idea of hollow, air-filled metal cylinders allowing people to descend hundreds or even thousands of feet into the inky blackness, while maintaining a perfectly livable atmosphere inside, is mind-bending.How did we ever figure that out? I want to not only ride inside a submersible, peering out through its thick acrylic view ports, but understand all the mechanics and engineering that went into its design and construction.Some of the most cutting-edge underwater exploration today is done by remotely operated vehicles, or ROVs - unoccupied robotic rovers controlled by teams of pilots and engineers aboard surface ships. I aspire to one day help map out new swaths of the seafloor using an ROV's high-definition cameras, sonar profilers and sampling tools.Who knows what natural marvels or archaeological wonders we could uncover? The remains of long lost ships and planes? New underwater species? Clues about the origins of life on earth or whether life exists elsewhere? The possibilities send my imagination into overdrive.Of course, exploring the ocean doesn't come without its risks. Scuba diving, especially of the extreme depth, polar or cave variety, carries any number of potential hazards. Decompressionsickness, oxygen toxicity, nitrogen narcosis and goodold-fashioned mechanical failures or human error could all potentially lead to serious injury or death in a worst-case scenario.Submarine and submersible operations carry their own suite of dangers as well. But for all their power and unpredictability, the oceans have always exerted a special pull over the human psyche - luring us to confront their mysteries. We simply can't resist the temptation to unlock their secrets, despite the inherent risks. With the proper training, equipment and safety protocols, those hazards can be responsibly managed.Sadly, the oceans and their wondrous inhabitants face grave threats themselves in the modern age. Plastic pollution,over-fishing, acidification, global warming and a host of other human-caused impacts are rapidly degrading marine ecosystems across the planet. Many species face extinction unless we change course soon.Part of my marine exploration goals involve witnessing these fragile environments firsthand before further damage is done, and hopefully using that experience to educate others about the need for greater conservation efforts. If we let the oceans die,we'll have done a profound disservice to every future explorer who yearns to glimpse their unseen realms.In the meantime, I'll keep preparing myself to eventually make that dream a reality. Maybe it will involve becoming a marine biologist or ichthyologist, studying and cataloging ocean species. Or an oceanographic researcher, helping to model and predict climate change impacts. Or an underwater video photographer or photojournalist, shining light on these ecosystems through vivid imagery.Or perhaps I'll end up taking a more hands-on path as a commercial diver, working in offshore oil and gas or operating submersible remotely operated vehicles. I could also join anon-profit organization committed to ocean conservation and educating the public. Heck, with some luck and hard work, maybe I could even become an aquanaut for NASA or a privateer space firm, testing the limits of underwater habitats in preparation for future space colonies.The direction I ultimately take remains to be seen, but my end goal is clear - to earn my way into the exclusive ranks of those relative few who have ventured into that alien world beneath the waves. To satisfy my lifelong fascination and bearwitness to the beauty, majesty and mysteries of the underwater realms firsthand.Most people in their lives never get to experience anything close to what lurks in the ocean's depths. The mere thought of drifting weightlessly hundreds of feet down, breathing a mixture of exotic gases pumped through an umbilical from the surface, is enough to make most people's head spin.But for those of us drawn to explore that frontier - to push the limits of the human experience and uncover the planet's last unseen places - the risk and hardship will be worth it a million times over. To gaze upon marine environments and life forms that have existed for eons before our species learned to walk upright, and will endure long after we're gone, is a humbling and transcendent privilege.I know in my heart that one day I'll be amongst those privileged few. I'll earn my way into those silent, eternal undiscovered countries and have my mind blown away by the indescribable wonders I encounter there. And when I return to the surface and remove my dive mask, I'll be a different person for having been one of the rare individuals across all of human existence to witness such splendor firsthand.For now though, I'll keep dreaming, training and preparing myself mentally and physically. Because the underwater world beckons. And I intend to answer the ocean's siren call one day soon.篇2A Hidden Wonderland Awaits: My Dream to Explore the Ocean's DepthsAs a young student fascinated by the mysteries of our planet, one place has captured my imagination more than any other –the vast, unexplored realm beneath the waves. The ocean, covering over 70% of the Earth's surface, harbors an entire world teeming with life and secrets just waiting to be uncovered. It's a hidden wonderland that I've only glimpsed through the pages of books and documentaries, but one that I dream of experiencing firsthand.Ever since I was a child, I've been mesmerized by the incredible diversity of marine life. From the vibrant coral reefs bursting with color and activity to the eerie, alien-like creatures that lurk in the deepest trenches, the ocean is a kaleidoscope of breathtaking sights. I can vividly picture myself donning awetsuit and scuba gear, descending into the cool, embracing waters to explore this extraordinary underwater universe.One of the main draws for me is the opportunity to witness firsthand the stunning array of marine creatures in their natural habitats. I yearn to swim alongside graceful manta rays, their enormous wingspans elegantly propelling them through the water. I long to come face-to-face with a gentle whale shark, the largest fish in the sea, and marvel at its sheer size and majesty. And who wouldn't be awestruck by the chance to observe a pod of dolphins, those intelligent and playful mammals, frolicking just an arm's length away?Beyond the captivating wildlife, the ocean's geological features hold an equal allure. I dream of navigating through intricate underwater cave systems, their walls adorned with ethereal stalactites and stalagmites formed over millennia. I imagine exploring shipwrecks, those time capsules frozen on the seafloor, each one holding untold stories and artifacts from bygone eras. And perhaps most tantalizing of all is the prospect of witnessing firsthand the towering hydrothermal vents, those deep-sea chimneys spewing forth superheated, mineral-rich waters that nourish entire ecosystems in the perpetual darkness.Of course, such an adventure would not be without its challenges. The immense pressures and frigid temperatures of the deep ocean would test my fortitude and resilience. There would be the ever-present risk of encountering venomous or predatory species, a humbling reminder of the ocean's unforgiving nature. And yet, these obstacles only serve to heighten the allure, for it is in conquering them that the true thrill of exploration lies.Beyond the sheer excitement of discovery, delving into the ocean's depths would also afford me invaluable opportunities for scientific research and environmental advocacy. As our planet faces the growing threat of climate change and pollution, understanding and protecting the marine environment has never been more crucial. By studying these ecosystems firsthand, I could contribute to our collective knowledge and efforts to conserve these precious underwater realms for future generations.In my mind's eye, I can already envision myself suiting up, taking those first few breaths from the scuba tank, and plunging beneath the surface. The world above would fade away, replaced by the mesmerizing blues and greens of the underwater realm. Schools of colorful fish would dart past, while towering kelpforests swayed hypnotically in the gentle currents. Every turn would reveal new wonders, from delicate coral polyps to fascinating invertebrates clinging to the rocky outcroppings.As a student, the opportunity to explore the ocean's depths represents the ultimate field trip, a chance to step beyond the confines of the classroom and immerse myself in the living, breathing laboratory that is our planet's final frontier. It would be an experience that would undoubtedly shape my perspective, ignite my curiosity, and fuel my passion for discovery.While the vast majority of the ocean remains unexplored and unknown to humanity, I hold steadfast to the dream that one day, I will have the privilege of contributing to its unraveling. Armed with determination, a thirst for knowledge, and an unwavering sense of wonder, I eagerly await the day when I can finally don篇3The Underwater World: A Hidden Realm I Long to ExploreEver since I was a little kid, I've been fascinated by the vast, mysterious realm that lies beneath the waves. Watching documentaries about the vibrant ecosystems teeming with incredible marine life, I was always awestruck by the sheer diversity and alienness of it all. The ocean seemed like an entirelydifferent universe, filled with wonders and secrets just waiting to be uncovered.As I grew older, my curiosity about the underwater world only deepened. I devoured books and articles about the exploration of the ocean depths, amazed by the tales of the intrepid scientists, researchers, and adventurers who ventured into that mysterious realm. Jacques Cousteau's chronicles of his groundbreaking underwater voyages were particularly enthralling, sparking my imagination with vivid descriptions of sprawling coral cities, kaleidoscopic schools of tropical fish, and bizarre, alien-like creatures from the darkest depths.More than anything, I longed to experience thosemind-boggling undersea marvels for myself. Sure, I could watch all the documentaries and read all the books in the world, but there's no substitute for actual immersion and first-hand encounters. That's why I've become utterly determined to find a way to explore the underwater world before I leave this earth.Just picture it - descending below the surface in a deep-sea submersible or donning high-tech diving gear, and being surrounded by the vast, azure expanse. Schools of shimmering fish dart past in mesmerizing formations, like an underwater murmuration. Graceful rays glide serenely along, carried bygentle currents. The vibrant, teeming cities of coral provide shelter to an entire universe of microscopic life. And who knows what enigmatic monsters lurk in the eternal midnight of the deepest trenches and ocean floors?Whenever I contemplate these sights, an almost overwhelming sense of awe and wonder washes over me. We've mapped the surface of Mars in greater detail than most of our own ocean floor! The depths represent one of the last great unexplored frontiers, filled with endless mysteries and unimagined marvels just waiting to be witnessed and studied by human eyes.As someone deeply fascinated by both biology and the natural world, the ocean exerts a magnetic pull that I can scarcely resist. Within those fathomless depths lies an entire alternate biosphere, home to some of the strangest, most remarkable life forms on our planet. Many of these creatures have adapted to the most extreme environments - from the crushing pressures of the oceanic trenches to the toxic, sulfur-choked rifts of hydrothermal vents. Their very existence seems to defy the boundaries of what we understand about the requirements for life itself.Just imagine witnessing an underwater volcanic vent firsthand, observing shrimp-like creatures the size of humans clustered around it as if drawn to some primordial heat lamp. Or floating past forests of striking, alien tube worms, theirruby-tipped plumes swaying hypnotically with the currents. These organisms derive their sustenance and energy not from the sun's rays, but from chemical compounds like hydrogen sulfide welling up from the rocks and microbial mats beneath. It's like glimpsing the life forms of an entirely alien world, right here on our home planet!Of course, the tantalizing allure of the ocean depths goes far beyond the chance to witness its bizarre denizens. For me, the sheer act of immersing myself in that aquatic realm would be a profound, almost spiritual experience. Can you imagine the silence and serenity of drifting weightlessly, suspended in that illimitable vastness? The muffled quiet, punctuated only by the occasional whale song echoing through the depths? The sense of peace and tranquility, utterly removed from the stresses and noise of the surface world?This sublime experience would also be a powerful reminder of our existence as fragile organisms, visitors in an element not truly our own. We surface-dwellers often take for granted the airwe breathe and the rhythms of the familiar world around us. But the ocean represents the true cradle of life on this planet - the primordial source from whence all terrestrial existence ultimately emerged. To journey into its depths would be to return, if only temporarily, to the womb that gave birth to us all.Of course, I understand that realizing this dream may be an immense challenge fraught with obstacles. Firstly, there's the profound expense of the specialized equipment and technology required, from research submersibles to advanced diving rigs. Exploring the deep sea, especially at its most extreme depths, necessitates gear and resources that stretch even the limits of what's currently available to science. Funding such expeditions is always an uphill battle, often dependent on government grants and the generosity of private donors.Then there are the very real dangers and risks inherent to underwater exploration. Even a relatively routine dive can turn hazardous in an instant due to potentially fatal factors like equipment failures, sudden changing currents, or marine life behaving unpredictably. As one descends deeper, the challenges only escalate - with intense water pressure capable of crushing even the most fortified research subs, dissolved gases becoming toxic at certain concentrations, and of course, the sheerinhospitability of an environment utterly devoid of light and warmth. A single, tiny miscalculation on an ultra-deep trench dive could spell immediate disaster.Still, despite the very reasonable trepidation such perils might inspire, my appetite for this unparalleled adventure remains utterly insatiable. The chance to gaze upon the wonders of the deep with my own eyes, to push the boundaries of human exploration, and to come face-to-face with the ocean's most bizarre and remarkable life forms - the potential rewards far outweigh any risks in my mind.And who knows? Perhaps in realizing my dreams of witnessing the marvels of the deep firsthand, I may even make some small contribution to humanity's understanding of this largely unexplored biosphere. Every single dive, every deep submersible voyage, yields new data and observations that could shed light on the ocean's complex dynamics and ecological systems. By personally experiencing and studying these environments, I may uncover revelations about the fundamental nature of life itself, with ramifications nobody could foresee.That tantalizing possibility alone is enough to inspire me to pour every ounce of effort into preparing for and undertaking this grand voyage of discovery. While the journey ahead willundoubtedly be fraught with immense challenges, I welcome those tests with open arms. For the chance to part the veil and behold the secrets of the deep blue - that hidden undersea universe we surface-dwellers know so little about - no price is too high. This dream represents the culmination of a lifelong obsession, and the key to quenching my relentless thirst for discovery and exploration.So here's to realizing that shimmering fantasy. One way or another, I will find my way beneath the waves and into that alien realm of the deep. And the wonders I bear witness to just may change the way we perceive our world forever. The clock is ticking, and my date with the briny depths awaits!。

以小见大形式的英语作文文语文三百字

以小见大形式的英语作文文语文三百字

以小见大形式的英语作文文语文三百字全文共3篇示例,供读者参考篇1The Little Things That Matter MostAs a student, I've come to realize that it's often the little things in life that make the biggest impact. These small moments and details may seem insignificant at first glance, but upon closer examination, they hold profound lessons and shape our experiences in meaningful ways.Let me start with something as seemingly trivial as a pencil.A simple pencil, with its unassuming wooden casing and graphite core, is an essential tool for any student. It allows us to take notes, jot down ideas, and capture our thoughts on paper. But a pencil represents so much more than just a writing utensil. It symbolizes the power of perseverance and the importance of preparation. Each time we sharpen that pencil, we're metaphorically honing our focus and determination to tackle the challenges that lie ahead.Moving on to a slightly larger scale, let's consider the humble backpack. This unassuming accessory is a constantcompanion for many students, carrying the weight of our academic pursuits – quite literally. Yet, a backpack is more than just a container for books and supplies; it's a symbol of the journey we're embarking on. The wear and tear on its straps, the faded patterns, and the scuffed corners tell a story – one of late nights spent studying, early morning commutes, and the relentless pursuit of knowledge.Expanding our perspective further, we arrive at the classroom itself. This familiar setting, with its desks neatly arranged and whiteboards adorning the walls, is where we spend countless hours absorbing information and engaging in intellectual discourse. But the true significance of the classroom lies not in its physical structure but in the connections forged within its walls. It's a place where ideas collide, perspectives are challenged, and lifelong friendships are born – all thanks to the seemingly mundane act of attending classes.Beyond the confines of the classroom, the school itself stands as a microcosm of the larger world. Its hallways and courtyards buzz with activity, a diverse tapestry of individuals coming together to learn, grow, and discover their passions. From the cafeteria's lively chatter to the library's hushed silence,each corner of the school holds its own story, its own lessons waiting to be uncovered.But the true power of these little things extends far beyond the boundaries of academia. They teach us valuable life lessons that transcend the walls of our educational institutions. The pencil reminds us of the importance of preparation and perseverance, traits that will serve us well in any endeavor we pursue. The backpack symbolizes the journey we're on, reminding us to embrace the challenges and cherish the experiences that shape our growth. The classroom and the school, in turn, emphasize the significance of community, collaboration, and the exchange of ideas – essential components of a fulfilling and meaningful life.As I reflect on these seemingly insignificant elements of my student life, I'm reminded of the profound impact they've had on my personal development. Each pencil stroke, each book carried, and each class attended has played a role in shaping who I am today and who I aspire to become.In a world that often prioritizes grandiose achievements and larger-than-life ambitions, it's easy to overlook the power of the little things. But it's precisely these small moments, theseseemingly trivial details, that lay the foundation for our successes and shape the trajectory of our lives.So, as I continue my journey as a student and beyond, I vow to appreciate the little things – the pencils, the backpacks, the classrooms, and the countless other seemingly insignificant elements that make up the tapestry of our existence. For it's in these small moments that we find the true essence of life, the lessons that guide us, and the inspiration to chase our dreams, one little step at a time.篇2Looking Back on the Pencil That Changed My LifeI was just a young student, probably around 8 or 9 years old, when a seemingly insignificant event dramatically shaped my outlook on life. It started with a simple pencil – a small, yellow pencil with teeth marks from years of nervous chewing. This pencil didn't seem like anything special at first glance. In fact, it was just about the most basic, unassuming pencil you could imagine. But in my young mind, it took on an almost mythical importance that day.It was nearing the end of the school year, and we had just taken a major test that would have a huge impact on ouryear-end grades. Naturally, the tension in the classroom was palpable as we awaited our scores. When the teacher finally handed back our graded exams, I couldn't resist the urge to peek at my result immediately. A huge smile spread across my face as I saw that I had aced the test – easily the top score in the class.Filled with youthful pride and wanting to shout my achievement from the rooftops, I enthusiastically raised my hand to share my good news with the teacher and the rest of the class. However, before I could say a word, something caught my eye that made me freeze. There, lying underneath the desk in front of me, was my pencil – the very same one I had been using to take that all-important exam.In an instant, my brain started racing. Had I accidentally dropped it while furiously bubbling in answers? Did it roll away from me without my knowledge? Regardless of how it got there, a sense of dread overcame me as I realized that leaving my pencil unmarked on the floor was a clear violation of testing protocols. Despite my academic success, I knew there was a strong chance that my test could be invalidated as a result of this minor oversight.As the teacher called on me, visions of summer school and heartbroken parents flooded my mind. I swallowed hard,deciding then and there to keep my mouth shut about my score rather than risk drawing attention to the pencil transgression. With my heart pounding, I simply mumbled "Nothing" and slumped back into my seat, crestfallen.In the days and weeks that followed, I silently stewed over the immense power that something as small and innocuous as a pencil had briefly wielded over my future. This tiny object, no篇3A Single Grain of RiceThey say that a journey of a thousand miles begins with a single step. But sometimes, the first step isn't even something you notice at the time. It can be as small and seemingly inconsequential as a single grain of rice.This particular grain first came into my life in an unassuming paper bag from the local grocery store. Just one of thousands nestled amongst its brethren, no way to distinguish it from the countless others. It remained that way as my mom measured it out into the rice cooker along with some water before clicking the switch to cook cycle. The aromas of jasmine rice filled the kitchen as the cooker worked its magic.I doubt the grain could have anticipated the grand adventure that lay before it when it was simply scooped out onto my plate amongst the steaming mounds of its peers. It was just part of the regular dinner routine in our house, rice to accompany some stir-fried vegetables and chicken. Something so ordinary, so ubiquitous across countless cultures and cuisines around the world that we hardly even think about it.Honestly, I can't even be sure which particular grain became The Grain as I shoveled forkfuls into my mouth between bites of the main dishes. Maybe it was one of the first ones, or maybe it joined the party a little later. But at some point, my chopsticks or fork lifted it from the plate and it became part of me.The digestive process brutally dismantled the grain's structure through mechanical and chemical means until it was broken down into simple glucose molecules. Those molecules were then absorbed into my bloodstream and carried off to my body's cells, including those in my brain.You might think that marked the end of this humble grain of rice's journey. But you'd be mistaken. Because that glucose provided the caloric energy that allowed me to stay awake and focused during my evening study session as I diligently prepped for my upcoming exams. Perhaps some of those molecules wereeven incorporated into new neurons and neural connections as I worked through problem sets and authored practice essays.In that sense, the grain became part of my growing knowledge, my ability to think critically and apply what I've learned. And it didn't stop there. In the days and weeks that followed, that grain continued doing its job as I took exams, wrote papers, and participated in class discussions and activities, all thanks to the efforts of that single grain of rice amongst the multitudes.Now you could make the argument that any grain would suffice, that there was nothing particularly。

制定规章制度翻译英语

制定规章制度翻译英语

制定规章制度翻译英语In order to maintain order and ensure the smooth operation of our organization, it is necessary to establish a set of rules and regulations that all members must abide by. These rules are designed to promote a safe and respectful environment for everyone involved, and to prevent any misunderstandings or conflicts from arising. It is important that all members familiarize themselves with these rules and adhere to them at all times. Failure to comply with the rules may result in disciplinary action.1. Code of ConductAll members are expected to conduct themselves in a professional and respectful manner at all times. This includes treating others with courtesy and respect, refraining from any form of discrimination or harassment, and following all relevant laws and regulations. Any form of inappropriate behavior will not be tolerated and may result in suspension or expulsion from the organization.2. Attendance and PunctualityAll members are expected to attend meetings, events, and other activities as required by the organization. If for any reason a member is unable to attend, they must inform the relevant authority in advance. Punctuality is also important, as late arrivals can disrupt the flow of the meeting or activity. Persistent tardiness may result in disciplinary action.3. ConfidentialityMembers are expected to maintain the confidentiality of all sensitive information shared within the organization. This includes personal information of other members, internal discussions, and any other privileged information. Breaching confidentiality may result in serious consequences, including legal action.4. CommunicationEffective communication is essential for the success of our organization. Members are encouraged to communicate openly and honestly with each other, to raise any concerns or suggestions, and to participate in discussions and decision-making processes. Respectful and constructive communication is key to maintaining a harmonious and productive environment.5. Conflict ResolutionIn the event of a conflict or disagreement between members, it is important to address the issue in a calm and professional manner. Members are encouraged to first attempt to resolve the conflict amongst themselves, and if necessary, seek assistance from a neutral mediator or higher authority. Any form of violence, aggression, or retaliation is strictly prohibited.6. Safety and SecurityThe safety and security of all members is of paramount importance. Members are expected to adhere to all safety protocols and procedures, to report any potential hazards or risks, and to take appropriate measures to ensure their own well-being and that of others. Any form of misconduct that jeopardizes the safety and security of others will not be tolerated.7. Compliance with PoliciesMembers are required to comply with all organizational policies, procedures, and guidelines. This includes but is not limited to financial regulations, conflict of interest policies, and social media guidelines. Failure to comply with these policies may result in disciplinary action.In conclusion, it is imperative that all members of our organization adhere to the above rules and regulations in order to maintain a positive and productive working environment. By following these guidelines, we can ensure the continued success and prosperity of our organization. Thank you for your attention and cooperation.。

Manpower Talent Pipeline Predictions说明书

Manpower Talent Pipeline Predictions说明书

Talent Pulse | September 2021 EditionPredicting the Talent Pipeline:As expanded benefits conclude, willworkers rush to come back?ManpowerThe U.S. workforce is still plagued with uncertainty as the pandemic ebbs and flows and expanded benefits are set to expire in September 2021 for many U.S. states.At ManpowerGroup, we ground our point of view from our experience in connecting hundreds of thousands of people to work each year and advanced data that provides actionable insights that allow us to best guide employers through these uncertain times. Employers today are asking if wages will continue to rise in September 2021, and whether they will see a surge in job candidates who are willing and able to work, making it easier to fill roles.Will wages continue to rise?Let’s start with the current situation. The economic recovery is happening faster than anticipated, but workers are taking longer to return to the labor market.Job openings reached 10.1 million in June, but the labor participationrate for July was 61.7%, lower than any year in the last 20+ years. There are multiple reasons for this. Part of the problem is a discrepancy betweenwhere openings are and what parts of the economy were hit the hardest.For example, service jobs are at a greater deficit than other industries.•Wage growth in the U.S. has been incredibly slow over the past 30 years. The federal minimum wage today sits at $7.25 per hour; in 1961 it was $1.15 which would be the equivalent of $9.88 per hour today.•However, in 2020-2021, wage growth accelerated at such a rapid pace – especially in blue-collar and manual work – that it resulted in gains we would expect to see over the course of a decade. Average wages grew by 4.0% from July 2020 toJuly 2021, much faster than what we would have expected to see pre-pandemic. Average Hourly Earnings of All Employees, Total Private•Major companies have raised their minimum wage to gain an advantage in the marketplace – Amazon, Costco, and Target are raising their minimum hourly wages to $15. In some cases, they’re advertising starting pay of $18 or higher. Jobs that pay at or close to the current federal minimum wage are increasingly difficult to fill.•Since the pandemic began, blue collar workers have received an average 6-18% increase in take-home pay.Based on the data, we expect wages will continue to increase as we anticipate demand to continue to outpace supply. Of course there will be variability by geographic location, industry and job type, however we are seeing larger companies setting the pace in a way that is moving the market – and this will require organizations of all sizes to be increasingly nimble in their attraction and retention strategies.The economic recovery is happening faster than anticipated, but workers are taking a longer time to returnto the labor market.Manpower Talent Pulse | September 2021 EditionWill I be able to fill my open roles?First, let’s look back to 2020 for guidance.•Covid-19 case surge history: Many are wondering what the relationship was between past surges and hiring patterns and what that tells us about howwe forecast going forward. Covid-19 surges happened at different times and in different parts of the country in 2020. While job opening rates across the country remained relatively steady month-over-month, hires roughly mirrored Covid’s geographical surges: in the Northeast (hardest hit in the first surge) hires were weakest in the spring and rose through the summer. In the South and West, hires fell slightly as the virus surged in July and August, and in the Midwest, hires fell off in the fall as Covid gained momentum.* Although we are seeing a surge in cases since July 2021, we expect increasing vaccination rates will help temper a peak in cases and impact on the workforce at the level we saw in Fall 2020 when vaccines were not yet available.•The school factor: What happened last September when kids went back to school? The Census Bureau reports that 93% of school age children reported some form of distance learning in 2020. Many children started this patternin the spring, however, so there was no associated rise in unemployment in September. And historically, there has been no associated nationwide flurry of job seekers returning to work at this time.It’s not likely that we will see an immediate flood of job candidates in September 2021 once many expanded benefits end, but rather a trickle that may increase over the following several months.What else is motivating/demotivating today’s workers? Unemployment Benefits: Many have suggested that extended unemployment benefits and the $300/week supplement have made people less likely to return to the workforce.Our July 2021 Talent Pulse reported that 1.8 million of the 14.1 million Americans receiving unemployment benefits had turned down jobs because of enhanced unemployment insurance benefits.However, in the 12 states that have already ended these benefits, the share of adults with jobs actually fell by 1.4 percentage points in these states according to CNBC and data from the Census Bureau.Manpower’s own data paints a similar picture: between the states that cancelled and states that have maintained the $300 supplement, there has been no notable change in the number of job advertisement responders and the number of candidates who engaged in an interview.We are finding that today’s job candidates are seriously considering other important factors when choosing when and where to work.New Work HabitsMany people have gotten used to cutting out the commute and spending more time with their families. In our What Workers Want report, 80% of U.S. workers told us they want options to work outside the office, while another 63% want flexible hours. In addition, 71% of U.S. workers reported that they want to prioritize time with family.Work-Life Balance orMore FlexibilityThe average worker’s work-life balance had been reportedly declining over the past several years. In many ways, the pandemic has awakened workers to the situation and motivated them to realign their priorities in a way that helps them achieve better work-life balance. Health and Safety Concerns The health and safety of workers and their families became a key consideration for job seekers during the pandemic, and remains critical.* Bureau of Labor Statistics. 2020 Job openings and Labor Turnover.12345What’s my best strategy?Here are some key ways you can position your company as a choice employer in order to compete for top talent while we remain in a talent-scarce market. Be wage competitive. We expect wages will continue to rise in this candidate-driven market. Consider raising yours. With a limited supply of qualified workers, we expect the supply of jobs will continue to outpace the supply of workers, adding to increased wage expectations. Just as important, don’t forget about your current staff. Consider raising wages 6-18% for current workers or offering retention bonuses. Our estimates show that it costs roughly 25% more to hire than to retain your current staff. Embrace flexibility. Workers are embracing One Life – a balance of work and home life. Although wages are a critical deciding factor, don’t overlook the value of offering a flexible work environment to attract and retain talent. That includes more time off and a remote or hybrid work schedule if feasible. Whatever you decide to do, make your policy crystal clear.Speed up onboarding. As of mid-July, Manpower tallied a 16%-24% no show rate . This is likely because in such a job-rich climate, candidates do not want or need to accept a lengthy hiring process. Do whatever you can to shorten the decision-making process by eliminating burdensome compliance requirements. Consider eliminating background checks or drug screenings, switch to oral vs. lab-based screenings, or conduct testing while the employee is already on the job.Cast a wider net . Look for transferable skills. For example, a candidate with retail sales experience may have the right transferable skills in customer service to work in a call center. Also lower minimum hiring standards, consider rehires, and specify skillset/resume must-haves versus “nice-to-haves”.Lead with Health and Safety protocols. Have clear mask/vaccination guidelines (knowing that local regulations may change). It’s important to have a written and enforceable policy that includes positions on vaccinations, mask wearing,and communication.Be sure to also communicate all of the added measures you are taking to help keep staff safe. In an online survey of nearly 1,800 U.S. workers conducted in August 2021, ManpowerGroup learned that cleanliness remains a key motivator for workers, as well, with 68% of respondents reporting they would be most comfortable working for an employer that demonstrates extra attention to a clean work environment. Another finding that could impact your approach is that of a subgroup of 1,245 survey respondents with an estimated average hourly wage of $15, vaccination rates fall 25% lower than the national vaccination rate for U.S. adults (46% vs. 62%) indicating that vaccination rates could vary widely based on take-home pay. Attracting and retaining the best talent today boils down to what you can offer workers. But first, you need to know where you stand amongst your competitors with regard to your ability to attract and retain talent. With this knowledge, you’ll be able to pull the levers that could impact your workforce.In the fight for talent, find out where you stand amongstyour neighboring companies and competitors with yourown Workforce Success Index Report. With a limited supply of qualified workers, we expect the supply of jobs will continue to outpace the supply of workers, adding to increased wage expectations.。

餐厅食品安全倡议书英语

餐厅食品安全倡议书英语

餐厅食品安全倡议书英语Food Safety Advocacy LetterDear [Restaurant/Establishment Name],We are writing to you as concerned consumers who value the importance of food safety in our community. As regular patrons of your establishment, we have noticed some areas of improvement that we believe will not only enhance the overall dining experience but also ensure the health and well-being of your customers.First and foremost, we urge you to prioritize staff training on food safety protocols and practices. This includes providing comprehensive training on proper food handling, storage, and preparation techniques. By equipping your staff with the necessary knowledge and skills, you can significantly reduce the risk of foodborne illnesses and contamination.Furthermore, we kindly request that you implement a strict quality control system. This should involve regularly inspecting all incoming ingredients, checking for their freshness, and ensuring they meet the required food safety standards. Additionally, establishing proper labeling and date coding systems will help you track the shelf life of your products and eliminate any potential risks.A crucial step towards ensuring food safety is maintaining a clean and hygienic environment. We suggest implementing a regular cleaning schedule and ensuring that all kitchen and dining areas are thoroughly sanitized. This should be complemented by enforcinggood personal hygiene practices among your staff, such as wearing hairnets, gloves, and maintaining clean uniforms.As customers, we believe in transparency and would appreciate it if you could display your most recent health inspection report in a visible location. This will foster trust and confidence amongst your patrons, assuring them that you take food safety seriously.Lastly, we encourage you to establish an open line of communication with your customers regarding any updates or changes related to food safety. This can be done through social media platforms, email newsletters, or even by displaying prominent signage in the restaurant. By keeping your customers informed, you will not only demonstrate your commitment to their safety but also encourage them to be proactive in practicing food safety measures.We understand that implementing these recommendations may require time and resources. However, we firmly believe that investing in food safety measures will not only protect your customers but also enhance the reputation and success of your establishment.Thank you for considering our concerns and taking the necessary steps to prioritize food safety. We look forward to continuing our patronage and recommending your establishment to friends and family with confidence.Sincerely,[Your Name][Your Address] [City, State, ZIP Code] [Email Address] [Phone Number]。

Mate_Plate酵母双杂交文库构建中文说明书(clontech)

Mate_Plate酵母双杂交文库构建中文说明书(clontech)

系统说明书PT4085-1 (PR013451)Cat. No. 630490Published February 2010“Mate & Plate™”文库构建系统说明书目录I. 介绍&操作流程 (4)II. 组份列表 (5)III. 附加产品推荐 (7)IV. 缩写列表 (8)V. 宿主菌株信息 (9)VI. 对照实验 (10)A. 总论 (10)B. 操作:对照同源重组克隆的实验操作 (10)VII. cDNA文库构建 (11)A. 操作:第一链cDNA合成 (11)B. 操作:长距离PCR (LD-PCR)扩增cDNA (13)C. 操作: CHROMA SPIN™ TE-400 纯化ds cDNA (14)VIII. 双杂交文库构建 (16)A. 酵母双杂交文库构建&筛选总论 (16)B. 操作:制作Mate & Plate文库 (16)IX. 参考文献 (18)X. 疑难解答指南 (19)附录A: 文库滴度测定 (24)附录B: 质粒信息 (22)附录C: 酵母生长培养基&补充物 (23)附录D: SMART™ 技术概述 (25)Protocol No. A Clontech Laboratories, Inc. Version No. PR013451 Takara Bio Company2“Mate & Plate™”文库构建系统说明书目录,续图表Figure 1.利用酵母的生物学知识构建Mate & Plate文库 (4)Figure 2. Mate & Plate文库构建概述 (11)Figure 3. SMART cDNA合成高质量cDNA (14)Figure4. CHROMA SPIN纯化柱和收集管 (15)Figure 5. pGADT7-Rec 载体图谱和克隆位点 (22)Table I:酵母宿主菌株的基因型 (9)Table II:根据不同SD培养基的表型测试 (9)Table III: RNA起始量和PCR最优循环数的相关性分析 (13)Table IV:酵母培养基套装2&酵母培养基套装2Plus的各组份 (23)Table V: Matchmaker黄金酵母操作用独立包装的酵母培养基 (23)Table VI:酵母双杂交筛选用的附加培养基&培养基补充物 (24)_____________________________________________A Clontech Laboratories, Protocol No. PT4085-1 Takara Bio Company Version No.PR0134513“Mate & Plate™”文库构建系统说明书1.介绍&操作流程酵母双杂交系统主要是从prey文库中筛选与已知蛋白(bait)有相互作用的蛋白(prey)。

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mention a repetition of effort for each agent engineer as each agent must be encoded with the same protocol. Recent research into multi-agent system protocols has begun to focus on first-class protocols, which were first defined in [14]. By first-class, we mean that a protocol exists as a computational entity in a multi-agent system that can be shared between agents, referenced, invoked, and composed, in contrast to hard-coded protocols, which exist merely as abstractions that emerge from the messages sent by the participants. Agents in the system read the definition of a protocol to learn it rules and meanings, and then structure their interaction from this definition. Authors of such work envisage systems in which agents have access to libraries of protocol specifications; either locally or centrally. Agents can search through these libraries at runtime to find protocols that best suit the goal they are trying to achieve, and can share these protocol specifications with possible future participants. If no single protocol is suitable for the agent, runtime composition of these may offer an alternative. This paper explores the current state-of-the-art in first-class protocol languages, and compare these. We look at four approaches: 1. In Section 3, we explore commitment machines, a socially-centric approach that uses commitment to define the meaning of messages and protocols. We review three different approaches [4, 5, 21], each of which uses a different language to define message sending and sequencing, but all of which share the common feature of using Yolum and Singh’s commitment machines framework [21]. 2. In Section 4, we explore the Lightweight Coordination Calculus (LCC). A protocol language described in [18]. Based on process algebra and logic programming, it utilises first-class protocol and logic programming techniques. 3. In Section 5, we explore the RASA language [14]. RASA is a framework for specification and reasoning about first-class protocols. The specification language combines process algebra and constraint languages to represent message sequences and message meaning respectively. 4. In Section 6, we explore an approach that uses extended Petri Nets [3]. This approach adapts Petri Nets [17] to specify message sequencing and message meaning. In Section 7, we compare and contrast the above approaches, outlining the relative advantages and strengths of the approaches.
Amongst First-Class Protocols
Tim Miller1 and Jarred McGinnis2
Department of Computer Science University of Liverpool, Liverpool, L69 7ZF tim@ 2 Department of Computer Science Royal Holloway, University of London, Egham, Surrey TW20 0EX jarred@
1
Introduction
Research into multi-agent systems aims to promote autonomy and intelligence into software agents. Intelligent agents should be able to interact socially with other agents, and adapt their behaviour to changing conditions. Despite this, research into interaction in multi-agent systems is focused mainly on the documentation of interaction protocols a priori. Agent developers use these documents to hard-code the interactions of agents. We identify three significant disadvantages with this approach: 1) it strongly couples agents with the protocols they use — something which is unanimously discouraged in software engineering — therefore requiring agent code to changed with every change in a protocol; 2) agents can only interact using protocols that are known at design time, a restriction that seems out of place with the goals of agents being intelligent and adaptive; and 3) agents cannot compose protocols at runtime to bring about more complex interactions, therefore restricting them to protocols that have been specified by human designers — again, this seems out of place with the goals of agents being intelligent and adaptive. An important corollary of these points is that the protocol is internalised within the individual agents. There is no possibility to communicate, inspect or verify the protocols by the agent or others. Not to
1
Abstract. The ubiquity of our increasingly distributed and complex computing environments have necessitated the development of programming approaches and paradigms that can automatically manage the numerous tasks and processes involved. Hence, research into agency and multiagent systems are of more and more interest as an automation solution. Coordination becomes a central issue in these environments. The most promising approach is the use of interaction protocols. Interaction protocols specify the interaction or social norms for the participating agents. However the orthodoxy see protocols as rigid specifications that are defined a priori. A recent development in this field of research is the specification of protocols that are treated as first class computational entities. This paper explores the most prominent approaches and compares them.
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