Experiments investigation of the effects of surface roughness on laminar flow in macro tubes
科学实验 英语
科学实验英语以下是关于“写科学实验”的相关英语内容:**单词**:1. "experiment" :实验;试验2. "science" :科学3. "write" :写;书写4. "conduct" :实施;进行5. "observe" :观察;观测6. "record" :记录;记载7. "data" :数据;资料8. "result" :结果;成果9. "procedure" :程序;步骤10. "hypothesis" :假设;假说**短语**:1. "write about a science experiment" :写关于一个科学实验2. "conduct a scientific experiment" :进行一个科学实验3. "observe the experiment" :观察实验4. "record the data" :记录数据5. "analyze the results" :分析结果6. "describe the procedure" :描述步骤7. "form a hypothesis" :形成假设8. "test the hypothesis" :检验假设9. "collect data" :收集数据10. "present the experiment" :展示实验**英语释义**:1. "Experiment" : A scientific procedure undertaken to make a discovery, test a hypothesis, or demonstrate a known fact.2. "Science" : The intellectual and practical activity encompassing the systematic study of the structure and behavior of the physical and natural world through observation and experiment.3. "Write" : To mark (letters, words, etc.) on a surface, especially with a pen or pencil, in order to communicate.4. "Conduct" : To organize and carry out (an activity or operation).5. "Observe" : To watch carefully and attentively.6. "Record" : To set down in writing or some other permanent form for later reference.7. "Data" : Facts and statistics collected together for reference or analysis.8. "Result" : Something that follows as a consequence or effect.9. "Procedure" : A series of actions or steps taken in order to achieve a particular end.10. "Hypothesis" : A supposition or proposed explanation made on the basis of limited evidence as a starting point for further investigation.**用法**:1. "experiment" 常用作名词,如:"The experiment was successful."(这个实验成功了。
(完整版)大学英语精读第6册课文全文翻译-中英对照
Problem Section. The first required section of a research report is the statement of the problem with which the research project is concerned. This section requires a precise statement of the underlying question which the researcher has set out to answer. In this same section there should be an explanation of the significance -- social, economic, medical, psychological, educational, etc. -- of the question; in other words, why the investigation was worth conducting. Thus, if we set out, for example, to answer the question "What is the effect of regular consumption of fast foods on the health of the American teenager?" we must explain that the question is thought to have significant relevance to the health of this segment of the population and might lead to some sort of regulations on such foods.
包衣存在的问题
药片包衣过程中常遇到问题及解决方法1、过湿过湿是由于喷液速度相对于干燥效率来讲过快,喷液与干燥之间没有达到平衡。
水溶性包衣机包衣过程中这一现象比较容易出现,因为水比有机溶剂难以干燥。
由于过湿,药片会粘在锅壁上,或是药片相互间粘在一起。
这样会造成衣膜破损,不均匀。
要避免过湿现象,必须仔细控制喷液速度,并可以增加进风温度。
另外在包衣液中加入滑石粉或硬脂酸镁,可以改善这一现象。
2、片面粗糙,不光滑多数情况下,是由于喷雾干燥造成的,因干燥太快时液滴不可能铺展。
另外,干燥了的聚合物颗粒粘在片芯的表面,易造成衣膜粗糙。
这一情况可以通过增加喷液速度、降低进风温度或缩短喷枪与片床之间的距离来改善。
3、桔皮现象这是衣膜表面粗糙的一种情况,液滴在片芯表面铺展不完全,在片芯表面不规则地分布有干燥的聚合物颗粒。
这主要由于干燥不当而引起,可以通过控制蒸发速度、增加喷液速度或缩短喷枪与片床之间的距离来避免这一现象。
4、架桥及填充现象当药片外表面不规则、刻有标识字母时,会发生表面的这些标识消失,被包衣液填充。
这通常是由于包衣液的粘度太大造成,增加溶剂量或增塑剂的用量,这种架桥或填充现象会消失。
5、衣膜颜色差异在许多处方中,有色素或染料,它们会悬浮在聚合物包衣液中。
如果搅拌太慢,会造成沉淀,导致批与批之间色泽差异。
温度过高,会使色素易于扩散,衣膜颜色变浅。
减少增塑剂用量,对包衣液进行搅拌,可以改进这一现象。
6、衣膜破裂这是在包衣中经常发生的问题之一。
聚合物衣膜破裂,出现裂缝,这种现象当问题发生时,可能会在包衣进行了几个小时后发生。
这是由于处方的原因所致。
在包衣液中加入分子量较大的聚合物,或是增加增塑剂的用量,会避免这一情况发生。
简而言之,成功的包衣取决于三个主要的因素:包衣处方:必须根据包衣的目的而定,要经过仔细筛选,也可以选用成品包衣材料,使用时配制;片芯:表面光洁度、硬度与脆碎度必须符合要求;设备:整个包衣过程中,必须能很好地控制主要工艺参数。
CRISPR技术基础和应用
Principle
Similarly to ZFNs and TALENs, Cas9 promotes genome editing by stimulating a DSB at a target genomic locus .
There are two major pathways for DNA damage repair : the error-prone NHEJ or the high-fidelity HDR pathway
In the CRISPR-Cas system derived from Streptococcus pyogenes SpCAS9,the target DNA must immediately precede a 5′-NGG PAM. While other Cas9 orthologs may have different PAM requirements, such as those of S. thermophilus (5′NNAGAA for CRISPR1 and 5′-NGGNG for CRISPR3) and Neisseria meningiditis (5′NNNNGATT)
For increased targeting specificity, an alternative strategy using the D10A nickase mutant of Cas9 (Cas9n) along with a pair of sgRNAs may be used. The D10A mutant Cas9 nickase (Cas9n) can be specified by a pair of appropriately spaced and oriented sgRNAs to simultaneously introduce single-stranded nicks on both strands of the target DNA.
做一顶实验的英语作文三百字
做一顶实验的英语作文三百字Designing an Experiment to Investigate the Effect of a Novel Compound on Cancer Cell Growth.Introduction.Cancer is a complex and devastating disease that remains a leading cause of death worldwide. Despite significant advancements in cancer research, effective treatments for many types of cancer are still lacking. Therefore, there is a critical need for the development of novel therapeutic strategies to combat this disease.Hypothesis.The hypothesis of this experiment is that a novel compound, designated as Compound X, will inhibit the growth of cancer cells.Materials and Methods.Cell Line Selection.The experiment will be conducted using a well-established human cancer cell line, such as HeLa or A549 cells. These cell lines are known for their rapid growth and ease of handling.Compound Preparation.Compound X will be prepared as a stock solution in a suitable solvent, such as dimethyl sulfoxide (DMSO). The concentration of the stock solution will be determined based on the solubility and potency of the compound.Cell Culture.Cancer cells will be seeded in 96-well plates and allowed to attach overnight. The cells will then be treated with varying concentrations of Compound X or vehiclecontrol (DMSO only) for 24, 48, or 72 hours.Cell Viability Assay.After treatment, cell viability will be assessed usinga standard assay, such as the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay measures the metabolic activity of cells, which correlates with cell viability.Data Analysis.The data from the cell viability assay will be analyzed using statistical software. Half-maximal inhibitory concentration (IC50) values, which represent the concentration of Compound X required to inhibit cell growth by 50%, will be calculated.Time Course Experiments.To investigate the time-dependent effects of Compound X, cells will be treated for different durations, ranging from 6 to 72 hours. The cell viability will be assessed at each time point to determine the optimal treatment time.Dose-Response Experiments.To determine the optimal dose of Compound X, cells will be treated with a range of concentrations, from 0.1 μM to 10 μM. The cell viability will be assessed to identify the concentration that produces the most significant growth inhibitory effect.Conclusion.The results of this experiment will provide valuable information about the potential of Compound X as a novel cancer therapeutic agent. If Compound X effectivelyinhibits cancer cell growth in vitro, it will warrant further investigation in preclinical animal models to assess its efficacy and safety in vivo.。
试验结果与讨论的英语
试验结果与讨论的英语Experimental Results and DiscussionThe scientific method is a fundamental approach to understanding the natural world, and it involves a systematic process of observation, hypothesis formulation, experimentation, and data analysis. In this paper, we present the results of a series of experiments conducted to investigate a particular phenomenon, as well as a discussion of the implications and potential applications of our findings.The primary objective of our study was to explore the relationship between two variables, A and B, and their impact on a specific outcome, C. We hypothesized that by manipulating the levels of A and B, we could observe a measurable change in the value of C. To test this hypothesis, we designed a set of experiments that involved systematically varying the levels of A and B while carefully controlling for other potentially confounding factors.In the first experiment, we examined the effect of varying the levels of A on the value of C, while keeping the level of B constant. Our results indicate that as the level of A increased, the value of C also increased in a linear fashion. This finding supports our initialhypothesis and suggests that A has a direct and positive influence on C.Next, we conducted a second experiment to investigate the influence of B on C, with A held constant. Our data reveal that as the level of B increased, the value of C exhibited a nonlinear, exponential relationship. Specifically, we observed a rapid increase in C at low levels of B, followed by a plateau and eventual decline as B continued to rise. This unexpected result suggests that the relationship between B and C is more complex than we had initially anticipated.To further explore the interaction between A and B, we designed a third experiment that involved systematically varying the levels of both variables simultaneously. Our findings demonstrate a significant synergistic effect, where the combined influence of A and B on C is greater than the sum of their individual effects. This observation highlights the importance of considering the interplay between multiple factors when studying complex systems.The results of our experiments have several important implications for both theoretical and practical applications. From a theoretical perspective, our findings contribute to a deeper understanding of the underlying mechanisms governing the relationships between A, B, and C. The nonlinear and synergistic effects we observed suggestthat the system under investigation is more complex than a simple linear model would suggest, and further research is needed to elucidate the precise nature of these interactions.On a practical level, the insights gained from our study could have significant implications for various fields, such as engineering, medicine, or environmental science. For example, the knowledge of how to optimize the levels of A and B to maximize the value of C could inform the design of more efficient systems or the development of new therapeutic interventions. Additionally, the understanding of the complex interplay between multiple factors could aid in the development of more accurate predictive models and the identification of potential leverage points for intervention.Despite the promising results of our experiments, it is important to acknowledge the limitations of our study and the need for further investigation. Our experiments were conducted under controlled laboratory conditions, and it is unclear how well the observed relationships would hold in real-world, complex environments. Additionally, we cannot rule out the possibility of other confounding variables that may have influenced the outcomes of our experiments.In conclusion, the results of our experiments have provided valuable insights into the relationships between A, B, and C, as well as the importance of considering the complex interplay between multiplefactors when studying natural phenomena. While our findings have significant theoretical and practical implications, additional research is necessary to further refine our understanding and explore the broader applicability of our conclusions. We believe that the continued pursuit of scientific knowledge through rigorous experimentation and thoughtful analysis is essential for advancing our understanding of the world around us and driving meaningful progress in various domains.。
英文文章回复审稿意见信2
REVIEW RESPONSE LETTER [33]Dear Reviewers,Thank you for reading our manuscript and reviewing it, which will help us improve it to a better scientific level. We revised our manuscript, and quite a lot of changes have taken place. So we have sent the revised manuscript, and a version containing all the changes to be visible.At the following, the points mentioned by the reviewers will be discussed:Reviewer 1:“The explanation that any other designs were ignored because of their complexity is unclear and insufficient, and should be extended (i.e.: why they were not considered?)”The introduction was edited to give an explanation on the reason of not investigating other designs. We mentioned that this is the design we consider, however other designs may be possible and even better! But they are not the subject of this investigation. Our objective was to give an explanation on the sources of the energy loss in this case, and we have done it.“The experimental part is very extensive, but not described enough.”We agree, so some explanations were added.“The weakest aspect of the paper is its lack of discussion on some of the results obtained. Explain what concl usions do you draw from the results (figures 6, 7, 8, 11).”The conclusion was written all over again, and some other parts were also added to give more discussion about the results.“Also, the effect of a number of parameters (type of balloon, its shape etc.) was neglected.”They were not neglected; this investigation was made in one specific condition. Investigation of the effect of all the parameters has not been an objective of this manuscript.“Equations should be centered in line.”Corrected.“First picture is unclear.”Changed.“The language is sometimes unclear or unspecific. In particular: “released energy its self”, “spent to fill”. You should write shorter sentences, and try to use non-hermetic phrases (“emptying diagrams”).”It was tried to be improved.“The authors use phrases that can be put in every solution to every task from the IYPT (“the physical experiments were also in charge to approve the theoretical assumptions). Be more specific.”We do not quite agree on this point. We still believe these classes of sentences are required, and indeed more specific detail has been provided after in the manuscript.“Figure 6: you are fitting a logarithmic function to your data. A linear fit to the same data would deliver a comparably high pre cision. Why did you choose the logarithmic fit?”The explanation was added to the manuscript. We want to use un-experimented points in the specific range that was experimented (and not out of this range). Thus the only thing that matters is howaccurately the interpolated function can predict these points. Since the logarithmic function had the highest regression, we used it.“What are the sources for the secondary peaks in figure 3 (top curve)?”Errors. The explanation is now added to the text:While measuring the Pressure, there were some errors caused by the dynamic motion of the water in the tube, particularly during the inflation process, in which the discharge could not be smaller than a specific amount. So at the beginning of the inflation process, where there is a maximum point for the pressure, the dynamic behaviour of the water would cause some oscillations in the water level; as visible in figure 3. However this error does not seem to be crucial since it is the integration of the P-V diagram which will be used later, and this oscillation does not have a significant effect.“What is the relative velocity of the jet? What do you understand by “jet”?”Corrected in the text to clarify.“Add references, especially to the topics mentioned above in ”references” part of review.”References were added, and addressed in the text.“chapter (five sentences approx.) with a discussion of li mitations of your solution (the description of limitations is scattered through the article)”Some explanation was added to the conclusion, describing the limitations.“sentence 1, paragraph 4 of introduction (“after when car is stopped…”) : The sentence is unclear. The reviewer understands the intention of using the energy conservation principle, but the description is not clear and should be changed or clarified.”Yes it was unclear; more explanation was added to clarify.“Describe your system and design. You use phrases “z-axis” without any description.”Figures were corrected to help.“Write a definition of efficiency that you use and clarify how the definition isunderstood.”a definition was added as the following:We define efficiency as the ratio of the work done on the car by the motivational force to the energy needed to fill the balloon.“The manuscript is recommended for publication only after essential revisions.”Thank you, we hope this revision has been enough.Reviewer 2:“Good structure. Very well presented. Please add references to the paper. I recommend this paper.”Thank you, references were added.Best Regards,Parham Zendehdel NobariReza Montazeri NaminHossein Azizinaghsh。
英语作文有关实验的题目
英语作文有关实验的题目Title: The Role of Experiments in Scientific Inquiry。
In the realm of scientific exploration, experiments serve as indispensable tools for probing hypotheses,testing theories, and unraveling the mysteries of the natural world. Through meticulously designed procedures and systematic observations, experiments offer a pathway to uncovering new knowledge and refining existing understanding. This essay delves into the significance of experiments in scientific inquiry, exploring theiressential role in advancing human understanding and driving innovation.To begin with, experiments provide a controlled environment where variables can be manipulated and outcomes measured with precision. This controlled setting allows scientists to isolate specific factors and observe their effects, thus enabling the establishment of cause-and-effect relationships. By carefully controlling variablesand conditions, researchers can minimize external influences and draw reliable conclusions about the phenomena under investigation.Moreover, experiments offer a means of testing hypotheses formulated through deductive reasoning or inspired by empirical observations. Hypotheses serve as educated guesses or tentative explanations for observed phenomena. Through experimentation, scientists subject these hypotheses to empirical scrutiny, either confirming or refuting their validity based on the evidence gathered. This iterative process of hypothesis testing lies at the heart of scientific inquiry, driving the advancement of knowledge and the refinement of theories.Furthermore, experiments foster a spirit of curiosity and exploration, encouraging scientists to push the boundaries of existing knowledge and explore new frontiers. By posing questions and designing experiments to address them, researchers embark on a journey of discovery, driven by a desire to unravel the mysteries of the universe. Whether exploring the depths of outer space or theintricacies of subatomic particles, experiments serve as vehicles for human ingenuity and exploration, fueling the quest for understanding.Additionally, experiments play a crucial role in validating scientific theories and models, providing empirical support for theoretical frameworks. Theories serve as overarching explanations for natural phenomena, offering insights into the underlying principles governing the universe. Through experimentation, scientists gather empirical evidence to either support or challenge these theories, refining and revising them in light of new data. This symbiotic relationship between theory and experiment forms the cornerstone of scientific progress, fostering a dynamic interplay between observation and theory-building.Moreover, experiments drive technological innovation and practical applications, translating theoreticalinsights into real-world solutions. From the development of new materials and medicines to the optimization of industrial processes, experiments underpin technological advancements that shape the course of human civilization.By applying scientific principles to practical problems, researchers harness the power of experimentation to drive innovation and improve the quality of life for people around the globe.In conclusion, experiments occupy a central position in the landscape of scientific inquiry, serving as catalysts for discovery, innovation, and understanding. By providing a systematic framework for hypothesis testing, empirical validation, and theory building, experiments empower scientists to unravel the mysteries of the natural world and push the boundaries of human knowledge. As we continue to harness the power of experimentation to explore new frontiers and tackle complex challenges, we embark on a journey of discovery that promises to enrich our understanding of the universe and transform the world we inhabit.。
螺旋桨飞机滑流对全机气动特性影响的试验研究
螺旋桨飞机滑流对全机气动特性影响的试验研究任庆祝;赵晓霞;刘毅;张鹏【摘要】为准确分析螺旋桨飞机有动力状态下的飞行性能和操纵品质,需要得到螺旋桨动力系统,特别是滑流对全机气动特性的影响量.采用一种“小天平+主天平”的多天平测量技术,可以分别测量得到螺旋桨的直接力和全机的气动力数据,进而得到比较准确的纯滑流对全机气动特性的影响量.通过螺旋桨飞机的带动力测力风洞试验研究表明:滑流会引起全机的升力系数、阻力系数,以及俯仰力矩系数相比无动力状态有所增加,升力系数和阻力系数增量随迎角大致呈二次曲线规律递增,俯仰力矩系数增量变化趋势与飞机所在迎角关系较大.滑流对横航向气动特性影响是降低了全机横向力导数以及横、航向静稳定性,并可能引起零侧滑下的全机的不对称横、航向力矩产生.【期刊名称】《科学技术与工程》【年(卷),期】2015(015)015【总页数】4页(P214-217)【关键词】滑流;气动特性;螺旋桨带动力风洞试验;多天平测量技术;桨盘系数【作者】任庆祝;赵晓霞;刘毅;张鹏【作者单位】中航飞机研发中心1所,汉中723000;中航飞机研发中心1所,汉中723000;中航飞机研发中心1所,汉中723000;中航飞机研发中心1所,汉中723000【正文语种】中文【中图分类】V211.7凭借出色的燃油消耗率、低廉的使用成本、较高的维修性和可靠性,以及对动力选择的多样化需求,涡轮螺旋桨发动机越来越广泛地被应用于航空飞行器中,如美军V-22“鱼鹰”运输机、E-2C“鹰眼”预警机、空中客车A400M运输机,以及安多诺夫AN-70运输机等。
目前我国已服役的多款运输机及特种飞机均采用了螺旋桨动力系统,滑流是螺旋桨动力飞机特有的一重要特征,研究滑流对飞机气动特性的影响对于准确分析飞机的飞行性能和操纵品质具有十分重要的意义。
螺旋桨动力系统对飞机气动特性的影响主要有直接影响和间接影响两部分[1]。
直接影响是由螺旋桨动力系统产生的拉力、扭矩等直接力影响,也包含飞机有迎角(或侧滑角)时气流斜吹螺旋桨后在桨盘平面内产生的螺旋桨径向力对全机气动特性的影响[2];间接影响是由螺旋桨滑流引起的全机气动特性的变化,螺旋桨滑流对全机的气动特性具有显著的影响[3—6]。
scientist studying the effects of
Scientists studying the effects of various phenomena play a crucial role in expanding our understanding of the natural world, human behavior, and the complex interplay between different variables. This process involves systematic investigation, data collection, analysis, and interpretation to draw meaningful conclusions. Let's explore the broader concept of scientists studying the effects of different factors and delve into the methodologies, significance, and challenges associated with such studies.### **Introduction:**Scientists engaging in the study of effects often aim to uncover causal relationships, identify patterns, or understand the impact of certain factors on a given system. This exploration encompasses a wide range of disciplines, including physics, chemistry, biology, psychology, environmental science, and social sciences. The effects being studied can be diverse, ranging from the microscopic level of particles to the macroscopic level of ecosystems or human societies.### **Methodologies in Studying Effects:**1. **Experimental Design:**- **Controlled Experiments:** Scientists often use controlled experiments to isolate specific variables and observe their effects systematically. This involves manipulating one variable while keeping others constant.2. **Observational Studies:**-**Longitudinal Studies:** Researchers track subjects over an extended period to observe changes and identify potential causative factors.- **Cross-Sectional Studies:** Examining a diverse group at a single point in time to uncover correlations and associations.3. **Field Studies:**-**Ecological Studies:** Scientists study effects within natural environments, observing interactions between organisms and their surroundings.-**Social Science Field Studies:** Researchers may conduct surveys or interviews to understand the effects of social, economic, or cultural factors on individuals or communities.4. **Computer Modeling:**- **Simulation Studies:** Scientists use computer models to simulate real-world scenarios, allowing them to predict and analyze potential effects without real-world experimentation.### **Significance of Studying Effects:**1. **Scientific Advancement:**- **New Discoveries:** Research on the effects of various factors often leads to the discovery of new phenomena, principles, or relationships.-**Advancement of Knowledge:** Building on existing knowledge, scientists contribute to the continuous advancement of their respective fields.2. **Problem Solving:**- **Environmental Solutions:** Studying the effects of human activities on the environment aids in developing strategies for sustainable resource use and conservation.- **Medical Breakthroughs:** Understanding the effects of drugs, diseases, and lifestyle on health contributes to medical advancements and improved healthcare.3. **Policy Formulation:**-**Informed Decision-Making:** Governments and organizations use scientific studies to formulate policies addressing societal issues, such as public health, education, and environmental protection.-**Risk Assessment:** Studying the effects of potential hazards helps in assessing and mitigating risks to human health and safety.4. **Technological Innovation:**- **Materials Science:** Studying the effects of different materials on each other contributes to the development of new materials with enhanced properties.-**Engineering Advancements:** Understanding the effects of forces, temperature, and other factors on structures and systems informs engineering practices and innovations.### **Challenges in Studying Effects:**1. **Complexity of Systems:**-**Interconnected Variables:** Natural systems are often complex, with numerous interconnected variables. Isolating the effect of one variable while keeping others constant can be challenging.2. **Ethical Considerations:**- **Human Subjects:** In social and medical studies, ethical considerations, such as informed consent and the potential for harm, must be carefully addressed.-**Environmental Impact:** Researchers studying ecological effects must consider the potential impact of their studies on the environment.3. **Resource Limitations:**-**Financial Constraints:** Conducting comprehensive studies requires financial resources for equipment, personnel, and data analysis.- **Time Constraints:** Longitudinal studies, in particular, can be time-consuming, requiring sustained funding and commitment.4. **Data Interpretation:**-**Statistical Challenges:** Interpreting data and drawing meaningful conclusions require statistical expertise to avoid misinterpretation or bias.- **Correlation vs. Causation:** Distinguishing between correlation and causation is critical to avoid drawing incorrect causal relationships.### **Case Study: Studying the Effects of Climate Change:**Consider a case study where scientists are studying the effects of climate change:1. **Methodology:**- **Observational Studies:** Scientists analyze long-term climate data, including temperature records, sea-level measurements, and ice core samples.-**Computer Modeling:** Climate scientists use sophisticated models to simulate future climate scenarios based on different emission scenarios.2. **Significance:**- **Policy Impact:** Findings contribute to global efforts to mitigate climate change, shaping international agreements and policy decisions.-**Environmental Awareness:** Studying the effects raises public awareness of climate change impacts, fostering environmentally conscious behaviors.3. **Challenges:**- **Data Uncertainty:** Climate systems are intricate, and uncertainties in data interpretation can pose challenges in predicting future scenarios.- **Global Collaboration:** Studying a phenomenon as pervasive as climate change requires international collaboration and coordination.### **Conclusion:**In conclusion, scientists studying the effects of various factors contribute significantly to human knowledge, technological innovation, and policy formulation across diverse disciplines. The methodologies employed, the significance of their findings, and the challenges they face vary depending on the field of study. Despite challenges, the pursuit of understanding the effects of different variables remains integral to scientific progress and addressing global challenges.。
An Investigation of the Effects of the
An Investigation of the Effects of the Core Protein Telomerase Reverse Transcriptase on Wnt Signaling in Breast Cancer CellsImke Listerman,Francesca S.Gazzaniga,Elizabeth H.BlackburnDepartment of Biochemistry and Biophysics,University of California at San Francisco,San Francisco,California,USATelomerase canonically maintains telomeres,but recent reports have suggested that the core protein mammalian telomerase reverse transcriptase(TERT)component,together with the chromatin remodeling factor BRG1and-catenin,may also bind to and promote expression of Wnt target genes.However,this proposed noncanonical role of TERT in Wnt signaling has been con-troversial.Here,we investigated the effects of human TERT(hTERT)on Wnt signaling in human breast cancer lines and HeLa cells.We failed tofind evidence for physical association of hTERT with BRG1or-catenin;instead,we present evidence that anti-FLAG antibody cross-reactivity properties may explain the previously reported interaction of hTERT with-catenin.Fur-thermore,altering hTERT levels in four different breast cancer cell lines caused minimal and discordant effects on Wnt target and Wnt pathway gene expression.Although hTERT’s role in Wnt signaling was addressed only indirectly,no significant repre-sentation of Wnt target genes was detected in chromatin immunoprecipitation-sequencing(ChIP-seq)and chromatin isolation by RNA purification and sequencing(ChIRP-seq)loci cooccupied in HeLa S3cells by both BRG1and hTR.In summary,our evi-dence fails to support the idea of a biologically consistent hTERT interaction with the Wnt pathway in human breast cancer cells, and any detectable influence of hTERT depended on cell type and experimental system.T he mammalian telomerase ribonucleoprotein complex adds TTAGGG repeats to telomeres,the ends of linear chromo-somes.The core human telomerase contains the catalytic reverse transcriptase protein component(hTERT)and the telomerase RNA(called hTR,hTER,or hTERC)that provides the template for telomeric DNA synthesis(1).In most human somatic cells, telomerase expression is very low.In contrast,telomerase expres-sion is upregulated in many human cancer cells and stem cells(2). In human cancer cells,the degree of telomerase expression seems higher than would appear necessary solely for maintaining telo-mere length.In fact,many studies suggest telomere-independent roles for telomerase.We and others have shown that overexpres-sion of TERT protects cells in culture from apoptosis indepen-dently of the telomere-lengthening properties of telomerase(3–5).Furthermore,overexpression of mouse and human TERT promotes cell proliferation in stem,normal,and cancer cell lines (6–11).Experiments employing overexpression or reduced ex-pression of hTERT in cells in culture have suggested roles for hTERT in controlling expression of growth factor response and other genes(9,12).Gene expression changes have been reported to occur as soon as1week after ectopic hTERT overexpression(9). Taken together,these results strongly suggest nontelomeric roles for telomerase;however,the mechanisms by which telomerase might protect against apoptosis and promote proliferation remain largely unknown.Some previous studies have linked TERT expression and Wnt/-catenin signaling,here referred to as Wnt signaling(13–15). The Wnt signaling pathway plays a central role in development, stem cell renewal,and cancer.In the absence of Wnt signaling, cytoplasmic-catenin is bound by destruction complex proteins, including AXIN,adenomatous polyposis coli(APC),and glyco-gen synthase kinase3beta(GSK3B).Consequently,-catenin is phosphorylated and degraded by the ubiquitin-proteasome path-way.When secreted Wnt proteins bind to Frizzled and low-den-sity lipoprotein receptor-related proteins(LRPs)at the plasma membrane,a signal is transduced to destabilize the-catenin de-struction complex.-Catenin can then translocate to the nucleus, where it complexes with T-cell factor/lymphoid enhancer factor (TCF/LEF)transcription factors to promote target gene transcrip-tion(16).The Wnt pathway has been previously shown to upregu-late telomerase in mouse mammary tumors and human cells(17, 18).Furthermore,-catenin may contribute to telomerase up-regulation in stem and cancer cells by directly regulating TERT expression via binding to the TERT promoter in complex with Klf4,as previously reported in a study of mouse adult stem cells and human carcinoma lines NTera2and SW480(15).Reciprocally,Park et al.previously suggested that TERT ex-pression promotes Wnt signaling(13).In that study,TERTϪ/Ϫknockout mice in thefirst generation were reported to have devel-opmental defects such as homeotic transformations of the vertebrae.Such defects,occurring before the onset of significant telomere shortening,resembled effects of aberrant Wnt signaling. Those authors additionally reported protein-protein interactions between hTERT and the chromatin remodeling factor BRG1and between hTERT and-catenin.It was also reported that TERT overexpression upregulated expression of a Wnt luciferase re-porter in TERTϪ/Ϫand TRϪ/Ϫmouse embryonicfibroblasts (MEFs)and humanfibroblast(BJ)cells and that,in SW-13and HeLa cancer cells,TERT overexpression hyperactivated a Wnt signaling reporter in a BRG1-dependent manner(13).Consistent with these results,Hrdlickováet al.reported increased prolifera-tion and a slight but significant increase in Wnt reporter activation Received28June2013Returned for modification6August2013Accepted4November2013Published ahead of print11November2013Address correspondence to Elizabeth H.Blackburn,Elizabeth.Blackburn@.I.L.and F.S.G.contributed equally to this article.Copyright©2014,American Society for Microbiology.All Rights Reserved.doi:10.1128/MCB.00844-13 Molecular and Cellular Biology p.280–289January2014Volume34Number2upon overexpression of either hTERT or a catalytically incompe-tent hTERT splice variant,in both U2OS(telomerase-deficient) and HeLa(telomerase-positive)cell lines(19).BRG1has been reported to bind to-catenin and to promote-catenin target gene expression(20,21).Because many growth-promoting genes are-catenin targets and because Wnt signaling plays an impor-tant role in self-renewal,proliferation,and survival,these reports suggested that TERT,in concert with BRG1,might promote cell proliferation via Wnt signaling.An influence of TERT on Wnt signaling has not been consis-tently reproduced in other experimental settings.Strong et al.did not detect homeotic transformations or diminished Wnt re-porter activity in TERTϪ/Ϫknockout mice or mouse embry-onicfibroblasts(MEFs)derived from these mice(22).The dis-crepancies between the two mouse TERTϪ/Ϫknockout studies could have been due to slightly different experimental condi-tions,such as different mouse backgrounds and/or Wnt signal-ing activators(13,22).Alternatively,the TERT overexpression system that identified a Wnt pathway interaction(13)may not produce a biologically relevant phenotype.While Strong et al. disputed a TERT/Wnt signaling interaction in mice and MEFs (22),they did not address a possible TERT/Wnt signaling in-teraction in human cancer cells.The primary aim of the present study was to determine whether hTERT promotes or otherwise affects Wnt signaling in cultured human breast cancer cells.Wnt signaling is often dys-regulated in breast cancer(23).Furthermore,either TERT over-expression or Wnt activation leads to mammary tumorigenesis in mice(23,24).We therefore focused on breast cancer cell lines to further study the potential for biologically relevant TERT/Wnt signaling interactions.MATERIALS AND METHODSCell lines.HeLa cells were purchased from the American Type Culture Collection(ATCC)and grown in Dulbecco’s modified Eagle’s medium (DMEM)with10%fetal bovine serum(FBS),1%penicillin-streptomy-cin,and1%GlutaMax(Invitrogen).HCC3153,HCC1806,SUM149PT, and MCF10A cells were obtained from the laboratory of Joe W.Gray, Oregon Health and Sciences University.HCC3153and HCC1806cells were grown in RPMI medium with10%FBS,1%penicillin-streptomycin, and1%GlutaMax(Invitrogen).SUM149PT cells were grown in Ham’s F-12medium with5%FBS,0.01mg/ml insulin,500ng/ml hydrocorti-sone,and1%penicillin-streptomycin.MCF10A cells were grown in DMEM–F-12with5%horse serum,20ng/ml epidermal growth factor, 100ng/ml cholera toxin,0.01mg/ml insulin,500ng/ml hydrocortisone, and1%penicillin-streptomycin.All cell lines were grown at37°C with5% CO2.Light microscopy.The HCC3153,HCC1806,SUM149PT,and MCF10A cell lines were grown on chamber slides(Lab-Tek II154526)and treated with25mM LiCl or200ng/ml Wnt3a(5036-WN-010/CF;R&D Systems)for4h.Cells werefixed in2%paraformaldehyde–phosphate-buffered saline(PBS)and permeabilized with0.5%NP-40–PBS.Immu-nostaining was performed with anti--catenin antibody clone14(BD Biosciences)followed by secondary Alexa Fluor488(Molecular Probes). DNA was visualized with4=,6-diamidino-2-phenylindole(DAPI;Invitro-gen).Images were acquired in0.5-m increments using a Deltavision RT deconvolution microscope(Applied Precision)with a100ϫ/1.40N PlanApo objective(Olympus).Images were deconvolved,Z-projected in Softworx(Applied Precision),and then adjusted for brightness and con-trast in FIJI(25).cDNA generation and qPCR.Total RNA was extracted with a Qiagen RNeasy minikit from cells treated with25mM LiCl,200ng/ml Wnt3a,or PBS for4h.cDNA synthesis was performed using2g RNA,random hexamers,and SuperScript III(Invitrogen).cDNA was amplified in10-l reaction mixtures containing LightCycler480DNA SYBR green I Master (Roche Applied Science)and a0.5to1mol/literfinal concentration of each primer using a Light Cycler480instrument(Roche Applied Science). The cycling conditions were95°C for5min,50cycles of95°C for10s and 60°C for20s,and72°C for20s.A melting curve(65to98°C)was gener-ated at the end of each run.Relative expression levels were determined by the2Ϫ⌬⌬CT method(26)and were normalized to GAPDH(glyceralde-hyde-3-phosphate dehydrogenase).AXIN2forward(5=-CATGTTCGTC ATGGGTGTGAACCA-3=)and AXIN2reverse(5=-TGGCTGGTGCAAA GACATAG-3=)and GAPDH forward(5=-CATGTTCGTCATGGGTGTG AACCA-3=)and GAPDH reverse(5=-ATGGCATGGACTGTGGTCATG AGT-3=)primers were used.For Wnt target gene expression analysis,cell lines were transduced with control or wild-type hTERT lentivirus pHR=cytomegalovirus(CMV)-hTERT-internal ribosome entry site (IRES)-PURO and selected with1g(HCC1806and SUM149PT)or2g (HCC3153)puromycin for3days and allowed to recover for1to2days. Cells were treated with25mM LiCl for6h,following total RNA extraction using an RNeasy kit(Qiagen)and cDNA generation using an RT2First Strand kit(Qiagen)according to the manufacturer’s instructions.A total of84Wnt target genes were measured using quantitative PCR(qPCR) human Wnt signaling target arrays(PAHS-243G;SABiosciences)accord-ing to the manufacturer’s instructions.A minimum cutoff of a2.5-fold change compared to control results was used to determine significant gene changes.Wnt luciferase reporter assays.The M50Super TOPFLash and M51 Super FOPFlash luciferase reporter vectors were obtained from Addgene (27).pRL-TK Renilla luciferase was used as an internal control(Invitro-gen).The lentivirus plasmids pBARL(-catenin activated reporter lucif-erase)and pfuBARL(mutated pBARL)and pSL9/rLuc(Renilla luciferase) were obtained from the laboratory of Randall Moon,University of Wash-ington(28).Cells were seeded on96-well microplates(655083;Greiner Bio-One),and each well was transiently transfected with0.5pg pRL-TK control along with either50pg SUPER TOPFLash or50pg mutated FOPFLash and with either10pg empty vector or10pg pcDNA3-FLAG-hTERT using X-tremeGene HP(Roche)and treated or not treated with25 mM LiCL for24h,followed by cell lysis with passive lysis buffer(Pro-mega)for10min and analysis using a dual-luciferase reporter assay sys-tem(Promega).Firefly and Renilla luciferases were read with a Veritas Microplate Luminometer(Turner Biosystems).Lentivirus production and transduction were carried out as previously described(28).Stable cell lines expressing reporter Renilla luciferase,pBARL,or pfuBARL lentivirus were generated as reported previously(28),using the same titer of lenti-virus in all cell lines.Then,cell lines were transduced with either control or hTERT lentivirus(28),selected with puromycin,treated with25mM LiCl for24h,and analyzed as described above.Background luciferase readings were subtracted,andfirefly luciferase values were normalized to Renilla luciferase.RNA interference(RNAi).Lentivirus expressing short hairpin RNAs (shRNAs)against-catenin(5=-CCGGAGGTGCTATCTGTCTGCTCT ACTCGAGTAGAGCAGACAGATAGCACCTTTTTT-3=;29),hTERT (5=-GGAGACCACGTTTCAAAAGTCTCTTGAACTTTTGAAACGTGG TCTCC-3=),and scramble shRNA(5=-GTTCTACAACGTAACGAGGTT TCTCTTGAAAACCTCGTTACGTTGTAGAAC-3=;30)was generated as described previously(30).Cells were transduced with shRNA and control vector lentivirus and were selected with1g/ml puromycin for3days and then expanded.IP and Western blotting.HeLa cells were transfected with pcDNA3 constructs containing wild-type hTERT with one N-terminal FLAG tag using Lipofectamine2000(Invitrogen)for18h,followed by treatment with25mM LiCl for6h.The following antibodies were used:anti-FLAG (M2;Sigma F3165and F1804),anti-BRG1(H-88;Santa Cruz),anti--catenin(clone14)(610153;Transduction Laboratories),and anti-GAPDH(MAB374;EMD Millipore).Cell lysis and immunoprecipitationhTERT Effects on Wnt Signaling in Breast Cancer281(IP)procedures and immunoblotting were done as described previously (13),using Western Lightning Plus ECL(PerkinElmer)for detection of horseradish peroxidase-conjugated secondary antibodies and Gamma-Bind G Sepharose(GE Healthcare Life Sciences)for preclearing and IP.Bioinformatics analysis.We determined the union of published BRG1-and hTR-enriched regions identified by chromatin immunopre-cipitation-sequencing(ChIP-seq)and chromatin isolation by RNA puri-fication and sequencing(ChIRP-seq),respectively,in HeLa S3cells(31, 32)and merged any united regions that were separated byՅ100bp using the ChIPPeakAnno R package(33).Enriched gene sets were obtained through use of the Genomic Regions Enrichment of Annotations Tool (GREAT)(34)on all145genomic regions.Gene ontology(GO)terms were identified using DAVID(35,36).RESULTSEndogenous Wnt signaling competency varies among the basal breast cancer cell lines SUM149PT,HCC1806,HCC3153,and MCF10A.One immortalized breast cell line with low telomerase activity and three basal breast cancer cell lines with midrange to high levels of telomerase activity(MCF10A[lower telomerase ac-tivity level],HCC3153[midrange telomerase activity level],and SUM149PT and HCC1806[higher telomerase activity levels]) were selected(4).First,to determine the extent of Wnt signaling in the breast cancer cell lines,we induced Wnt signaling with Wnt3a or LiCl.Wnt3a activates the pathway at the cell surface receptor level and specifically induces-catenin signaling by binding to Frizzled and LRP receptors(37).LiCl pharmacologically inhibits GSK3B kinase activity in the cytoplasm,thus leaving-catenin un-phosphorylated and stabilized(38).Under control conditions,both SUM149PT and HCC3153cells showed diffuse cytoplasmic and nuclear-catenin staining,suggesting that they may have had dysregulated Wnt signaling,which is found frequently in breast cancers(39).Upon Wnt3a orLiCl treatment,SUM149PT and HCC3153cells showed strongernuclear localization of-catenin(Fig.1A).In accordance with theincreased nuclear localization of-catenin in those cells,bothtreatments increased the expression of the endogenous AXIN2-catenin target gene(Fig.1B).In contrast,in HCC1806cells,-catenin was largely membrane bound and remained so even after LiCl or Wnt3a treatment(Fig.1A).Consistent with thesefindings,HCC1806cells did not significantly upregulate AXIN2after Wnt signaling induction(Fig.1B).In MCF10A cells,-catenin was also largely membrane bound but showed weak nuclear localization after LiCl or Wnt3A treatment(Fig.1A),and AXIN2was moderately upregulated(Fig.1B).These results sug-gest that HCC1806cells are not competent for Wnt signaling in-duction by LiCl or Wnt3A.We conclude that Wnt signaling can be activated in SUM149PT and HCC3153lines,and somewhat less in MCF10A cells,but at most minimally in HCC1806cells.hTERT overexpression has minimal and nonconcordant ef-fects on Wnt signaling reporters in breast cancer cell lines.Hav-ing established that HCC3153,SUM149PT,and MCF10A but notHCC1806cancer cells can strongly to moderately activate Wntsignaling,we tested whether hTERT overexpression modulatedWnt signaling reporter genes in these lines,as has been reportedfor MEFs,HeLa cells,and U2OS cells(13,19).For independentFIG1Endogenous Wnt/-catenin target gene induction varies in breast cancer cell lines.(A)Cell lines SUM149PT(high telomerase),HCC3153(medium telomerase),HCC1806(high telomerase),and MCF10A(low telomerase)were treated with PBS,25mM LiCl,or200ng/ml Wnt3a for4h prior to staining for -catenin(green)and DAPI(blue).(B)The increase in Wnt/-catenin target gene AXIN2mRNA expression over that of PBS control-treated cells was measuredby qRT-PCR following activation with Wnt3a or LiCl for4h.Listerman et al. Molecular and Cellular Biologyverification,we employed two different Wnt signaling reporter construct systems with multimerized TCF/LEF binding sites driv-ing luciferase expression:the M50Super TOPFlash reporter and its corresponding control M51Super FOPFlash Wnt reporter(27) and the BARL(-catenin activated reporter luciferase)/fuBARL (control)system(28).While TOPFlash contains7TCF/LEF bind-ing sites and was transiently expressed via plasmid transfection, BARL contains12TCF/LEF binding sites and was stably inte-grated.Vector or hTERT plasmids were cotransfected together with TOPFlash/FOPFlash in SUM149PT,HCC5313,HCC1806, MCF10A,and HeLa cells,followed by LiCl treatment(Fig.2A).As expected,LiCl treatment strongly increased luciferase activity in the vector-transfected TOPFlash SUM149PT,HCC3153, MCF10A,and HeLa cells.LiCl treatment induced the luciferase activity in HCC1806cells only weakly,with maximum luciferase expression being5-to100-fold lower than in the other four cell lines,consistent with our observations(Fig.1),indicating that HCC1806cells are severely impaired in Wnt signaling.Further-more,only HCC3153cells exhibited a statistically significant but mild(ϳ1.4-fold)increase in relative luciferase activity over that of the vector control,while the relative luciferase activity did not change significantly compared to that of vector controls in SUM149PT,HCC1806,MCF10A,or HeLa cells.In the pBARL cells,overexpressing hTERT increased luciferase activity over that of the vector control cells only in HCC3153cells(byϳ2-fold) (Fig.2B).Overexpression of hTERT did not detectably change the luciferase activity in SUM149PT,HCC1806,or MCF10A cell lines expressing pBARL.In HeLa cells that stably expressed the TCF/ LEF mutant(control)binding site construct,fuBARL,with hTERT overexpression,we observed anϳ3-fold increase in lucif-erase activity over that of control cells.However,in HeLa cells stably expressing BARL,there was an onlyϳ2-fold increase in luciferase activity in hTERT-overexpressing cells over that of control cells(Fig.2B).Hence,hTERT overexpression activated luciferase expression regardless of the presence or absence of a functional TCF/LEF promoter in HeLa cells.Because hTERT overexpression led to mild hyperactivation of both Wnt signaling reporters only in HCC3153cells and not in the four other cell lines,we conclude that Wnt reporter hyperactivation through hTERT is dependent on the context.Hence,hTERT does not hy-peractivate Wnt reporters universally but instead does so in a cellline-and context-dependent manner.Lack of evidence for hTERT interaction with-catenin orBRG1in HeLa cells.Since Park et al.(13)reported that FLAG-hTERT in HeLa cells coimmunoprecipitated(co-IP)with BRG1,aprotein previously reported to interact with-catenin(13,20),wealso investigated hTERT/Wnt pathway interactions using HeLacells.To independently verify the previously published results(13),we transiently overexpressed FLAG-hTERT in LiCl-treatedHeLa cells and tested whether BRG1or-catenin interacted withFLAG-hTERT by coimmunoprecipitation(co-IP).Interestingly,using the same buffers as described by Park et al.(13)and thenon-affinity-isolated version of anti-FLAG antibody M2(F3165;Sigma)for IP,we observed a strong band migrating slightly slowerthan the-catenin band in Western blots(M2lanes in Fig.3A)when the FLAG-IP Western blot was stained with the-cateninantibody.Importantly,we also observed the same band in similarquantities independently of whether FLAG-hTERT was expressedin the HeLa cells(M2lanes in Fig.3A)and using a variety ofwashing procedures in the IP and Western blot experiments.Weextended these experiments using the affinity-isolated anti-FLAGM2antibody(F1804;Sigma),which,while it did not enrich forthis background band,instead detected another band of the ex-pected size for-catenin at low levels(1.7-fold over IgG control IPresults)that again were identical regardless of whether FLAG-hTERT was expressed(Fig.3B).We verified the identity of thiscross-reacting coimmunoprecipitated protein band as-cateninby RNAi:reducing-catenin expression produced correspondingreductions in the intensity of the band pulled down by the affinity-purified M2antibody co-IP experiments(Fig.3C).Thus,we didnot detect a significant or specific interaction between hTERT and -catenin in HeLa cells above the background signals caused by anti-FLAG antibody ing the same antibodiesand IP buffers as Park et al.(13),we were also unable to detect aninteraction between overexpressed FLAG-hTERT and endoge-nous BRG1,despite obtaining high signals corresponding toFLAG-hTERT itself with the FLAG antibodies used(Fig.3B).In addition,we were able to detect only a weak interaction,atbest,between endogenous levels of BRG1and-catenin;suchan interaction has previously been reported only in a BRG1FIG2Effect of hTERT overexpression on two Wnt/-catenin reporters.(A)SUM149PT,HCC3153,HCC1806,MCF10A,and HeLa cells were transiently transfected with pRL-TK Renilla luciferase vector(internal control),hTERT or vector,and M50SuperTOPFlash or M51SuperFOPFlash reporter vectors and treated or not treated with25mM LiCl for24h prior to luciferase measurement.*,PϽ0.05.(B)Cell lines were transduced with pSL9/rLuc,pBARL,or pfuBARL and either hTERT or vector control lentivirus and selected for stable expression prior to LiCl treatment and luciferase measurement.hTERT Effects on Wnt Signaling in Breast Cancer 283overexpression context(20).We conclude,first,that the en-dogenous expression levels of BRG1in HeLa cells were too low to detect strong interactions with-catenin in our experi-ments,second,that M2anti-FLAG antibody cross-reacts with a protein with a gel mobility close to that of-catenin,and third, that the interaction of FLAG-hTERT with BRG1or with -catenin was not significantly above background IP levels.BRG1and hTR do not colocalize at Wnt target genes.In hu-man cells,the hTERT protein and the telomerase RNA hTR,to-gether with additional proteins,assemble to form the telomerase ribonucleoprotein complex,although it has not been determined what fractions of the total levels of hTERT and hTR exist in these complexes.Previously,Chu et al.(31)used whole-genome chro-matin isolation by RNA purification(ChIRP)in HeLa S3cells to detect hTR associated with chromatin at2,198genomic locations in HeLa S3cells.Those authors additionally reported that the hTR-bound peaks they had identified were significantly enriched at loci of genes in the“Wnt receptor signaling pathway”gene ontology(GO)term and on this basis proposed that hTR in com-plex with hTERT cooccupies Wnt target genes(31).Given the evidence described above that a previously reported interaction between hTERT and-catenin protein can be explained by cross-reactivity of anti-FLAG antibody rather than a bonafide interac-tion,we used a bioinformatics approach to reexamine any poten-tial connection between the published genomic localizations of BRG1cross-linked sites,telomerase RNA cross-linked sites,and Wnt signaling genes.To identify loci on the HeLa S3genome enriched for localization sites of both BRG1and hTR(with hTR inferred to likely be in complex with hTERT,as was described previously[31]),we merged the published BRG1-enriched local-ization sites identified by ChIP-seq in HeLa S3cells(32)and the published hTR-enriched localization sites(31).We applied the criterion that they were separated byՅ100bp,as the same crite-rion was previously used to determine cooccupancy of BRG1with other members of the SWI/SNF complex at genomic loci(32). Using this criterion,217genes in the vicinity of the merged BRG1/ hTR-enriched loci were identified.However,while“Positive reg-ulation of apoptosis”was identified as a highly significant GO term among these217genes,Wnt signaling was not identified as a significant GO term(Table1).Of the217genes,only MYC is known to be a target gene of Wnt signaling.Thus,this analysis in HeLa S3cells(applying the criterion of peak separation no greater than100bp)failed to verify any significant cooccupancy by hTR and BRG1of Wnt target genes or their nearby controlling regionsFIG3hTERT does not interact with-catenin or BRG1.(A)Anti--catenin antibody cross-reacts with anti-FLAG immunoprecipitate.HeLa cells were either transfected with pcDNA3-FLAG-hTERT for16h(left)or left untransfected(right),followed by treatment with25mM LiCl or no LiCl treatment.Precipitates from anti--catenin(clone14)or anti-FLAG(M2antibody F3165,Sigma)IP were subjected to Western blotting(WB)with anti--catenin(clone14)or anti-FLAG antibodies.The asterisk indicates a slower-migrating background band.(B)Top:Western blot of HeLa cells transfected with FLAG-hTERT(right hand side)or left untransfected(left hand side)following IP with specific antibodies.M indicates size marker lane.Bottom:Western blot of input samples from experiment.(C)Top:Western blot of HeLa cells treated with or without-catenin shRNA following IP with specific antibodies.Bottom:Western blot of input samples from the experiment.Listerman et al. Molecular and Cellular Biologyexcept for MYC.This analysis does not directly address hTERT and BRG1cooccupancy on chromatin genomic loci.However, because some fractions of hTERT and hTR exist as telomerase complexes in HeLa S3cells,colocalization of hTR with BRG1at Wnt signaling gene loci might be predicted if hTERT protein and BRG1protein interact,as suggested previously(31).Our negative finding for hTR and BRG1cooccupancy at Wnt signaling gene loci thus does not support,but by itself does not refute,the possibility of interaction between hTERT and BRG1.Effects of hTERT overexpression on Wnt signaling target gene expression in cell lines do not reflect cellular Wnt signaling competency.While our bioinformatics data analysis did not iden-tify a significant overlap of Wnt pathway genes with genomic loci cross-linkable to hTR and BRG1in HeLa cells,this analysis did not exclude the possibility that TERT,possibly not bound to hTR,still promotes Wnt signaling.To test directly whether hTERT modu-lates endogenous Wnt signaling target gene expression in breast cancer cells,we stably overexpressed hTERT in the high-Wnt-signaling SUM149PT and HCC3153cells and,as a control,in the Wnt-signaling-impaired HCC1806cells.We then measured the mRNA levels of84endogenous Wnt downstream target genes supplied as arrays for quantitative RT-PCR(qRT-PCR)(see Ma-terials and Methods).First,with hTERT overexpression(Fig.4C), the Wnt-signaling-competent SUM149PT cells showed a modest overall trend to greater expression of the Wnt target genes as a group compared with vector controls;however,this trend was no greater than that seen for the Wnt-signaling-impaired HCC1806TABLE1Significant GO terms for genes close to BRG1and hTR co-occupied loci in HeLa S3genomeGene GO term P valueGO:0043065Positive regulation of apoptosis0.005GO:0006928Cell motion0.025GO:0051693Actinfilament capping0.027GO:0007028Cytoplasm organization0.035GO:0006917Induction of apoptosis0.035GO:0048146Positive regulation offibroblast proliferation0.037GO:0030834Regulation of actinfilament depolymerization0.040GO:0033043Regulation of organelle organization0.043GO:0032272Negative regulation of protein polymerization0.045GO:0032271Regulation of protein polymerization0.046FIG4Effect of hTERT overexpression on Wnt target gene expression in breast cancer cell lines.SABiosciences qPCR arrays were used to measure the effect of hTERT overexpression on the mRNA expression of endogenous Wnt target genes after treatment with25mM LiCl for6h.(A)Scatter plots of log-transformed relative expression levels of each gene.Red lines indicate a2.5-fold change in gene expression.(B)Wnt target genes that changedϮ2.5-fold compared to the control results(indicated by dotted line)upon hTERT overexpression in SUM149PT,HCC1806,and HCC3153cells.(C)hTERT mRNA expression relative to GAPDH mRNA and vector control expression in SUM149PT,HCC1806,and HCC3153.Bars represent means of the results of3(SUM149PT and HCC1806)and 2(HCC3153)biological replicates.Error bars indicate standard deviations(SD).hTERT Effects on Wnt Signaling in Breast Cancer 285。
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ACADEMIC PHRASEBANKABSTRACTMotivation...Problem StatementAn experimental investigation was conducted to explore …The effect of …… is numerically investigated for Reynolds number range of …This article provides numerically study of the …..In this study, ….. was analyzed.This article presents an exhaustive review of these studies and suggests a direction for future developments.Approach… is investigated by a numerical study.ResultsThe results reveal that …Based on the results, ….The calculation in this work suggest that ….The results also show that …The results of the numerical simulation indicate that the ….It was found that …Conclussion…, and areas for future research are recommended.Implications of the results and future research directions are also presented.The results presented here may facilitate improvements in the …INTRODUCTIONEstablishing the importance of the topic:The topic of natural convection in enclosures is one of the most active areas in heat transfer research today ...One of the most significant current discussions in nanotechnology is ....The knowledge of the X in Y is important for an understanding of ...The X has been investigated by many researchers using ...In the recent decades, X has been one of the major interesting research subjects due to ...In the past two decades, X spaces have been studied extensively.Recent developments in X have heightened the need for ....In recent years, there has been an increasing interest in ....Recent developments in the field of X have led to a renewed interest in ....Recently, researchers have shown an increased interest in ......The past decade has seen the rapid development of X in many ....The past thirty years have seen increasingly rapid advances in the field of ....Over the past century there has been a dramatic increase in ....In the past years, the X has attracted much attention as a ...The primary goal of turbine cooling research during the past years was to developFilm cooling on turbine components has been studied since the 1970’s, and...X has been studied over the latest few decades because ...General descriptions of the relevant literature:Researchers have studied the effect of ...Previous studies have primarily concentrated on …There have been several studies in the literature reporting ….A considerable amount of literature has been published on X. 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[1-3].A number of study analyses have examined the relationship between .... [1-3] The relationship between X and Y has been widely investigated [1-3]The causes of X have been widely investigated [1-3].It has been suggested that .... [1-3]It has conclusively been shown that .... [1-3]Previous research has shown that (2)Studies have found that …. [1-3].More recent studies have confirmed that …. [1-3]Time frame referencesIn 1975, Author et al. published a paper in which they described (4)In 1990 Author et al. demonstrated that (4)Thirty years later, Author reported that (4)In the 1970s Author pointed that (4)In 1981, Author and co workers demonstrated that (4)In 1990, Author et al. reported (4)In 1984 Author et al. Concluded that (4)Author as subjectAuthor identifies ....Author lists three possibilities....Author traces the development of ....Author provides in-depth analysis of the work of ....Author highlights the need to....Author discusses the challenges and strategies for ....Author mentions the siginificant relationship between .... Author questions whether ....Author argued that/the ….Author carried out numerical study of ….Author describes ….Author discovered ….Author found that ….Author has demonstrated that ....Author have proposed ….Author indicated that ….Author investigated on….Author investigated the ....Author measured the ….Author noted that ....Author observed that ….Author posited that ….Author proposed ….Author raised several concerns about …. They suggested that…. Author reported that/the ....Author researched the ….Author showed … .Author studied ….Author suggested that ....Author summarized that ....Author presented ....Author extended this work to ….Author presented a comprehensive review of …. Author performed experimental investigations on … Author performed numerical analysis of …Author analyzed ….Author studied the … Their analysis revealed that …. Author reviewed the available literature on …Research topic as subject…, such as the works of Author et al.were carried out by Author.was done by Authorwere investigated by Author.was attemted by Author… is analyzed by AuthorOthersFor example, Author found that….Recently, Author gave a comprehensive review on …. In a different study, Author examined….In another study, Author examined ....Another studies by Author....According to Author….According to an investigation by Author, ….The study by Author examined the ….A study by Author suggests there should be ...As highlighted by Author, ….According to Author, ....Study by Author found that ….…. model was used by Author to do research on ….…. model was used by Author to predict ….The study of Author was the ….With the same objective, Author conducted numerical experiments on …..More recently, Author investigated ….Early studies by Author ….Highlighting contradict findingsHowever, a number of studies show that significant differences do exist, albeit findings are somewhat contradictory.Author found differences suggesting that….In contrast, Author concluded that ....In contrast, the study by Author indicated that ....The above findings contradict the study by Author. 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Furthermore, it can be seen from the limited works on …..No attempt was done to explore the potential of ….Most of the open literatures on the …..were focused mainly on the …..In addition to these primary data, systematic studies on the ….are still needed for better understanding of …A careful study of the literature reveals that the …..has not been thoroughly investigated. Aclear understanding of ….is very essential for …It may however be noted that nost od the studies were aimed at …. and a rigorous study for ….has not been attempted to.On …..one unresolved question is why ….Nevertheless, few studies are to be found providing detailed ….Most previous studies have …., however, it is essential to provide ….However, …. are still lacking.There have been relatively few recent studies on …..It should be noted from the above literature review, however, that limited studies are available on…… and this has motivated the present study.It should be noted, however, that all the previous studies of ….that are reported in the open literature …… To the best of author knowledge, the case of …. has not been given great attention by the researchers in the past and this motivated the present study.Although the above invetsigation examined the ….., to the best of author knowledge, only few reference in the literature systematically describe the effect of. …. 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Therefore, another motivation for this study is to …In summary, many interesting results indicating the potential of …. have been reported.However most of the studies in the open literature did not simultaneusly examine the effect of …To the author best knowledge, previous studies on … only focussed on …..Although these investigations reported many interesting results, litle work has examined ….In particular, the effect of ….remains unexplored.A survey of literature on …. is scarcely reported.Focus, Aim, Objective:The objective of the present work paper is to investigate ...In this work we propose a simulation which uses ...The objective of this study is to develop ...This paper will focus on/examine/give an account of ....The objectives of this paper are to determine whether ....This paper seeks to address the following questions:This paper critically examines/discusses/traces ....The aim of this paper is to determine/examine ....The aim of this study was to evaluate and validate ....The present study examined numerically the ....This study was spawned from the lack of research of ....The objective of this paper is to numerically study ....Therefore, this study aims to experimentally investigate the ....Therefore, the present study carried out the ....The primary goal of this study was to determine the ....The current study involves an investigation of the effects of ....Accordingly, the present study attempts to…The aim of the present work is to investigate the effects of ….The paper also explores … The current study attempts to cover a wide variety of…….The goal of this study is develop a more rigorous understanding of …The present study considers ….The present study explores the influence of …The present study focuses on …The present work is an extension of the work reported earlier by Author with emphases on ..The purpose of this article is to…..The purpose of this study is to determine….The purpose of this study was to ….Therefore, the intention of the present study is to ….This paper researched the …..This study contributes to the extant literature in several ways. First …This study tested the hypothesis that …This study was undertaken to …This paper attempts to provide a more detailed investigations regarding the effects of … While the objective of the present investigation focus on ….the long term implications of this study will impact....In this study, …..has been investigated .The present work compares ....This paper continues the effort of Author to understand the ….The purpose of this research was ….In this paper, ….. is proposed.Thus, the present study deals with ...Hence the goal of this paper is to find out if there exist a possibility for improving the heat transfer using …..which was not favored in prior attempts by ….The main aim of the present study is to investigate …This work presents …The present study is motivated by the need to take into consideration the …The purpose of this paper is to review recent research into the ....This paper will review the research conducted on ....This paper reviews the literature concerning the usefulness of using .... 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First, ….The long term implications of this study will impact ...The uniqueness of this study exists in the fact that …To demonstrate the potential of this approach and its suitability for the application, ….is investigated.RESULTS AND DISCUSSIONDiscussion of ResultsThe discussion of the results begin with …This finding highlights…The finding of the present study suggest thatThe findings suggest that …..The finding provides evidence that …This study indicates that …The results of the present study also suggest that..The present findings also suggest that …Our finding revealed that …Among the plausible explanations for these findings is that ….The most striking result to emerge from the data is that ......Interestingly, this correlation is related to .....The correlation between X and Y is interesting because ......The more surprising correlation is with the ......The single most striking observation to emerge from the data comparison was ......Comparisons of Results with previous findingsThe present finding also support Author study which concluded that ....The finding is consistent with findings of past studies by Author, which…. 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Is given in Table 1.CONCLUSSIONRestatement of aimsThis study set out to determine ....The present study was designed to determine the effect of ....In this investigation, the aim was to assess ....The purpose of the current study was to determine ....This project was undertaken to design .... and evaluate ....… are investigatedThis paper presented results of …Summarising the findingsThis study has shown that ....These findings suggest that in general ....One of the more significant findings to emerge from this study is that .... It was also shown that....This study has found that generally ....The following conclusions can be drawn from the present study .... 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Further work needs to be done to establish whether ....It is recommended that further research be undertaken in the following areas: Further experimental investigations are needed to estimate ....What is now needed is a study involving ....More broadly, research is also needed to determine ....Further research might explore/investigate ....Further research in this field/regarding the role of X would be of great help in ....Further investigation and experimentation into X is strongly recommended. A number of possible future studies using the same experimental set up are apparent. 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实验与试验区别作文
实验与试验区别作文英文回答:Experiment and trial are two terms commonly used in scientific research and investigation. While they may seem similar, there are distinct differences between the two.An experiment refers to a carefully planned and controlled procedure that is conducted to test a hypothesis or validate a scientific theory. It involves manipulating variables and measuring the resulting effects. The purpose of an experiment is to gather empirical evidence and draw conclusions based on the observed data. Experiments are often conducted in a laboratory setting, where researchers have control over the conditions and variables being studied.On the other hand, a trial can be described as a test or examination of something. It is a broader term that encompasses a range of activities, including experiments.Trials can be conducted in various fields, such as medicine, law, and technology. Unlike experiments, trials are not necessarily conducted under controlled conditions and may involve real-world scenarios or situations. The goal of a trial is to evaluate the effectiveness, safety, orfeasibility of a particular intervention, product, or process.In summary, experiments are a specific type of trialthat follows a systematic and controlled approach to test a hypothesis or validate a theory. Trials, on the other hand, encompass a wider range of activities and can be conductedin different fields with varying degrees of control and rigor.中文回答:实验与试验是科学研究和调查中常用的两个术语。
2023-2024学年湖南省长沙市第一中学高三上学期月考(五)英语试题
2023-2024学年湖南省长沙市第一中学高三上学期月考(五)英语试题Driving has always been my escape. And then I have kids. Kids change the whole freedom of the road experience. They arrive in your backseat one day, like some crying VIPs that you need to carry around, and nothing is ever quite the same again.These days, with three kids between the ages of 1 and 6 lined up in the backseat, I find that driving my car has become more about predicting what might erupt at any moment. So, for those of you about to become parents, here are some types of kids you’ll have in your car.1. The Complainers“Are we there yet?”“Why is it taking so long?”“I’m so bored that I hate your fat face, Dad!”When kids are tired or feel a little annoyed, they complain. And nothing can make a car ride worse than a steady stream of complaint coming from the backseat.2. The BoxersOnce you have two kids back there who’ve reached the age of 3 or 4, you can be absolutely certain that, if they are within even a long arm stretch of one another, they will hit each other in the face more frequently than most parents ever want to admit.3. The Hungry VikingsMy kids attack the snack and let it fall anywhere they may. I don’t know why I continue to let my kids eat in the car. Maybe it’s because we’re on the go a lot? Maybe it’s because I’m a complete idiot? Regardless of the real reason, the fact of the matter is that kids under, say 15 seem to eat food in a moving vehicle much the same way that I imagine hungry Vikings used to tear a barely-cooked turkey leg whenever they set foot on shore after a long period at sea.4. The ConversationalistsKids are really great traveling companions a lot of the time-especially when they’re fast asleep. But when they’re just sitting back there talking to you, listening to you, and smiling at you in the rearview mirror (后视镜), those are the best times in the world.1. What has changed the author’s driving experience?A.Getting married. B.Dealing with VIPs.C.Carrying kids in the car. D.Having naughty kids in the family.2. Why does the author call little kids boxers?A.They usually practice boxing in the car.B.They usually fight each other like boxers.C.They always pretend to be boxers.D.They always watch boxing matches in the car.3. When the author describes kids in the car as Vikings, he really means ________.A.they are always hungryB.they have little food to eat in the carC.they throw food everywhereD.they behave wildly like stealing thingsThirty-four years after graduating from high school, I found myself sitting with some of my former classmates for an all-school reunion.Our conversation centered around funny things and what our classmates were up to now. Eventually, the conversation came to a classmate, Janelle. Jill said, “Has anyone been in touch with Janelle?” Diana said, “I tried to contact her for our last reunion. She never responded.” Jill said, “I’ve always felt bad about how she was treated.” There was a general murmur of agreement around the table.It had started in elementary school when Danny, a popular kid who was always looking to be the center of attention, caught a glimpse of Janelle picking her nose. Unaware that she was being watched, she slipped her finger into her mouth. Immediately, Danny pointed at Janelle and exclaimed, “She ate her boogers (鼻屎)! THAT is SO disgusting.” The boys had a “grossed out”(恶心的) look on their faces and the taunt (嘲弄) was repeated by every boy. By the end of the next day, everyone in our class had known Janelle’s new nickname —“booger eater” and called it at least once. Reluctant to join in, somehow we did it anyway.“Janelle never did anything. She just looked sad. Unfortunately, no one else did anything either. I wish any one of us had stood up for her,” said Jill. Everyone around the table agreed.Each of us shared a time when we were reminded of Janelle over the years. Jill shared how her own daughter was bullied in school. Diana shared how her son came home from school and told her about his classmate being bullied, and they talked about how he could stand up for the child being bullied. I shared how we taught acceptance and kindness in my school. Several of us had tears in our eyes as we talked. We all regretted the part we played in the bullying.When it was time to leave, Diana said, “I want to believe that, even though we cannot make amends (补偿) directly to Janelle, we can at least forgive ourselves. Let’s make an agreement going forward to do small acts of kindness in Janelle’s honor. We hugged each other tightly with damp eyes and agreed. I said, “I’m glad something good came out of that neg ative experience that happened so long ago.” Although forgiveness didn’t occur the way we wanted it to —with Janelle— forgiving ourselves feels good. We can’t change the past, but we can forgive and move forward.4. Why did Jill feel bad about how Janelle was treated?A.Jill was a close friend of Janelle’s.B.Jill unwillingly called Janelle “booger eater”.C.Jill regretted not standing up for Janelle.D.Jill was the one who started the nickname.5. What was the outcome of the reunion for the classmates?A.They vowed to change the past.B.They decided to contact Janelle again.C.They determined to be kind to others.D.They apologized to Janelle for their behavior.6. What life lesson did the author learn?A.Forgiving others is forgiving ourselves.B.Demonstrating regret is of great importance.C.Reunion with classmates helps reflect ourselves.D.We should learn from the past and strive to be better.7. What is the best title for the text?A.No Bullying B.Behaving OurselvesC.Seeking Forgiveness D.Bidding Farewell to the PastVast stretches of America are dominated by corn, nearly 100m acres of it, stretching from Ohio to the Dakotas. What once was forest or open prairie (大草原) today produces the corn that feeds people, cattle and, when made into ethanol (乙醇), cars.Now, the na tion’s airlines want to power their planes with corn, too. Their ambitious goal would likely require nearly doubling ethanol production, which airlines say would slash their greenhouse gas emissions. If they succeed, it could transform America’s Corn Belt yet again, boosting farmers and ethanol producers alike, but also potentially further damaging one of the nation’s most important resources: groundwater.Corn is a water-intensive crop and it can take hundreds of gallons to produce a single gallon of ethanol. But as airlines embrace the idea of ethanol, prompting lobbyists (游说者) for ethanol makers and corn growers alike to push for clean-energy tax credits in Washington, vital aquifers (地下水层) face serious risks. “We’re on track to massively increase water usage without any real sense of how sensitive our aquifers are, “ said Jeffrey Broberg, who is concerned about groundwater in Minnesota. a major corn state, where he is a water-use consultant and founder of the Minnesota Well Owners Organization.United Airlines this veal signed a deal with a Nebraska ethanol company to buy enough sustainable aviation fuel as the biofuel is known, to power 50, 000 flights a year. In August, Delta announced a plan to create a sustainable fuel hub(中心) in Minnesota. The Biden administration could decide onits tax incentives (激励) for the industry as soon as December. “Mark my words, the next 20 years, farmers are going to provide 95% of all the sustainable airline fuel, ’’President Biden said in July.This year a New York Times data investigation found that groundwater is being dangerously exhausted nationwide, largely by agricultural overuse. As climate change makes rainfall less reliable and intensifies droughts, rising demand for ethanol could put even more pressure on Amer ica’s fragile aquifers to be used for irrigation.8. What does the underlined word “slash” mean in paragraph 2?A.Increase. B.Decrease. C.Maintain. D.Transform.9. What is the potential risk of the increased use of ethanol in aviation fuel?A.Decreased water usage.B.Increased reliance on aquifers.C.A boost in clean-energy tax credits.D.Expansion of. corn production.10. What can we learn from paragraph 4?A.Farmers will double their ethanol production.B.The sustainable aviation fuel will not gain popularity in the future.C.Farmers will play a major part in the sustainable aviation fuel industry.D.The Biden administration opposes the new aviation fuel to protect the aquifers.11. What is the main idea of the text?A.The Biden administration’s a ttitude to the corn and ethanol industry.B.Using corn-based ethanol can significantly reduce greenhouse gas emissions.C.Groundwater resources are being dangerously exhausted due to agricultural overuse.D.Using corn-based ethanol in aviation may lead to increased groundwater exhaustion.Why do people read negative Internet comments and do other things that will obviously be painful? Because humans have an intense need to resolve uncertainty according to a recent study in Psychological Science. The new research reveals that the need to know is so strong that people will seek to satisfy their curiosity even when it is clear the answer will hurt.In a series of experiments, behavioral scientists at the University of Chicago and the Wisconsin school of Business tested students willingness to expose themselves to unpleasant stimuli in an effort to satisfy curiosity. For one trial, each participant was shown a pile of pens that the researcher claimed were from a previous experiment. The twist? Half of the pens would deliver an electric shock when clicked. Twenty-seven students were told which pens were electrified, another half were told only that some were electrified. When left alone in the room, the students who did not knowwhich ones would shock them clicked more pens and suffered more shocks than the students who knew that would occur.Subsequent experiments reproduced this effect with other irritants, such as the sound of fingernails on a chalkboard and photographs of disgusting insects.“The drive t o discover is deeply rooted in humans, much the same as the basic drives for food or shelter, “says Christopher Hsee of the University of Chicago. “Curiosity is often considered a good instinct — it can lead to new scientific advances, for instance — but sometimes such inquiry can backfire. ”The insight that unhealthy curiosity can drive you to do self- destructive things is a profound one.In a final experiment, participants who were encouraged to predict how they would feel after viewing an unpleasant picture were less likely to choose to see such an image. These results suggest that imagining the outcome of following through on one’s curiosity ahead of time can help determine whether it is worth the endeavor. “Thinking about long-term consequences is key to reducing the possible negative effects of curiosity,” Hsee says. In other words, don’t read online comments.12. What can we learn about the experiment by behavioral scientists in paragraph 2?A.The total of the subjects is 27.B.All the pens were not electrified.C.Equally many shocks happened to all the subjects.D.No subjects were exposed to the pens previously.13. What does the underlined word “irritants” in paragraph 3 mean?A.Stimulants. B.Solutions. C.Criteria. D.Purposes.14. What is the attitude of Christopher Hsee towards curiosity?A.Approving. B.Dismissive. C.Doubtful. D.Objective.15. What is paragraph 5 of the text mainly about?A.The cause of unhealthy curiosity.B.The negative impact of unhealthy curiosity.C.The underlying feature of unhealthy curiosity.D.The practical measure to resist unhealthy curiosity.You know the feeling--your ears start to warm up, your tongue goes numb (麻木的), and you start sweating and taking deep breaths. You’ve just eaten something spicy, know ing it would be painful, but you chose to do it anyway. Are humans just masochistic (自讨苦吃的), or is there something else going on? 16 .Spicy isn’t actually a taste like salty, sweet, sour and bitter-- it’s a sensation. 17 . When we eat foods containing capsaicin (辣椒素), our bodies are tricked into thinking the temperature is actually rising. In trying to temper the burning sensation, our bodies release endorphins (内啡肽) which control pain and, at the same time, give a feeling of pleasure---like painkillers.This is what is happening chemically, but there is also a conscious side to choosing spicy food. Dr Tamara Rosenbaum, Cognitive Neuroscientist at the National Autonomous University of Mexico, explains in an interview with the BBC that this is 18 . Furthermore, we obtain pleasure from the other ingredients chilli is generally cooked and eaten with- including fat, sugar and salt. So-like skydiving-eating, chilli is a form of thrill-seeking, 19 .This masochistic relationship with capsaicin started in the Andes of South America, where chilli peppers originate. Humans were one of the few mammals on Earth that developed a taste for capsaicin. As archaeological evidence suggests, they started cultivating chilli peppers about six thousand years ago. Human intervention changed the chilli pepper to suit human tastes and needs--including the pepper’s colour, size and capsaicin content-helping to explain the many different types of chilli peppers now available. 20 . We eat around 57. 3 million tons of peppers globally each year, and chilli is a key ingredient in traditional dishes from Mexico to Korea. Maybe we are masochistic after all.One night I decided to spend some time building a happier and closer relationship with my daughter. For several weeks she had been _______ me to play chess with her, so I suggested a game and she eagerly _______. It was a school night, however, and at nine o’clock my daughter asked if I could _______ my moves, because she _______ to go to bed; she had to get up at six in the morning. I knew she had strict bedtime, but I thought she ought to be able to _______ some of this strictness. I said to her, “Come on, you can _______ late for once. We’re having fun.” We played on for another fifteen minutes, during which time she _______ her fingers on the chessboard. Finally she said, “Please, Daddy, do it quickly.” “No”, I replied, “If you’re going to play it well, you’re going to play it _______.” And so we continued for another ten minutes, _______ suddenly my daughter burst into tears, and ________.Clearly I had made a(n)________. I had started the evening wanting to have a(n)________ time with my daughter but had allowed my desire to win to become more ________ than my relationship with my daughter. When I was a child, my desire to win ________ me much. As a parent, I ________ that it got in my way. So I had to change.21.A.training B.asking C.driving D.advising 22.A.waited B.apologized C.responded D.accepted 23.A.slow B.repeat C.accelerate D.check 24.A.agreed B.managed C.desired D.pretended 25.A.treasure B.ensure C.adjust D.expand 26.A.warm up B.stay up C.wake up D.turn up 27.A.put B.burned C.drummed D.crossed 28.A.cautiously B.frequently C.randomly D.rapidly 29.A.until B.as C.now that D.for fear that 30.A.stepped into my shoes B.threw in thetowelC.made ends meet D.took the upperhand31.A.appointment B.attempt C.difference D.mistake 32.A.subsequent B.awkward C.brief D.awesome 33.A.crucial B.casual C.practical D.reliable 34.A.depressed B.benefited C.dominated D.annoyed 35.A.recognized B.added C.denied D.explained阅读下面短文, 在空白处填入1个适当的单词或括号内单词的正确式。
宁波市自然科学基金项目 英文
宁波市自然科学基金项目英文Title: Investigation of the Impact of Environmental Factors on the Biodiversity of Ningbo CityAbstract:\nThe biodiversity of Ningbo City, located in the eastern coastal region of China, is facing significant threats due to rapid urbanization and industrialization. To address this issue, the Ningbo Natural Science Foundation has initiated a research project to investigate the impact of environmental factors on biodiversity in the city. This project aims to provide valuable insights into the conservation and sustainable management of natural resources inNingbo.Introduction:\nNingbo City is known for its rich natural resources and diverse ecosystems. However, with the rapid expansion of urban areas and industrial activities, these ecosystems are under increasing pressure. The objective of this research project is to identify and understand the key environmental factors that influence biodiversity in Ningbo City.Methods:\nThe research will employ a multidisciplinary approach, combining field surveys, laboratory experiments, and data analysis. Field surveys will be conducted across various habitats within Ningbo City to assess species diversity and abundance. Additionally, environmental parameters such as temperature, humidity, soil composition, and air quality will be boratory experiments will be conductedto investigate the effects of specificenvironmental factors on selected indicator species. These experiments will provide valuable insightsinto how changes in environmental conditions affect biodiversity at a microscale level.Data analysiswill involve statistical modeling techniques to identify correlations between environmental factors and biodiversity patterns. Geographic Information System (GIS) technology will also be utilized tomap species distribution across different habitats within Ningbo City.Expected Results:\nThe research project expects to yield several important findings. Firstly, it aims to identify key environmentalfactors that significantly influence biodiversityin Ningbo City. This knowledge can then be used to develop effective conservation strategies for protecting vulnerable species andhabitats.Furthermore, this project aims to providea comprehensive understanding of how changes in environmental conditions impact different taxonomic groups within Ningbo's ecosystems. This information can guide policymakers in making informed decisions regarding land-use planning and environmental management.Conclusion:\nThe investigation of the impact of environmental factors on the biodiversity of Ningbo City is a crucial step towards ensuringthe long-term sustainability of its natural resources. By identifying key factors influencing biodiversity, this research project will contributeto the development of effective conservation strategies and sustainable management practices. The findings will also serve as a valuable resource for policymakers, researchers, and environmentalists working towards preserving the unique biodiversity of Ningbo City.。
我做了物理实验英语作文
我做了物理实验英语作文英文回答:In the domain of experimental physics, the meticulous execution of experiments can unveil profound insights into the intricate tapestry of natural phenomena. I embarked on a comprehensive experimental investigation, meticulously adhering to the scientific method. The experiment entailed the systematic manipulation of independent variables to elucidate their effects on dependent variables.Through meticulous data collection and rigorous analysis, I established clear and quantifiable relationships between the variables, affirming a causal connection. The outcomes of the experiment provided empirical support for preexisting theoretical frameworks and, in some instances, necessitated a reassessment of established paradigms.The meticulously crafted experimental design isolatedthe variables of interest, effectively eliminating confounding factors that could have potentially compromised the integrity of the findings. The precision with which measurements were taken ensured the reliability andvalidity of the data, allowing for meaningfulinterpretation and robust conclusions.The experiment not only served as a crucible fortesting hypotheses but also as an invaluable educational tool. It engendered a profound understanding of the scientific process, from the formulation of research questions to the dissemination of findings. By actively engaging in the scientific method, I developed a heightened appreciation for the rigor and discipline required in the pursuit of knowledge.In conclusion, the meticulously executed experimental investigation provided valuable insights into the relationships between the variables under scrutiny. The experiment reinforced the power of the scientific method and ignited a passion for further exploration in the enigmatic realm of physics.中文回答:实验物理论文:我开展了一项全面的实验研究,严格遵循科学方法。
《科学探究的过程》高中生英语作文
《科学探究的过程》高中生英语作文The Process of Scientific InquiryScientific inquiry is a systematic process that involves observing, questioning, and investigating to understand natural phenomena.It is a fundamental part of the scientific method and is crucial for advancing human knowledge.The process of scientific inquiry typically involves several steps, which include making observations, asking questions, developing hypotheses, conducting experiments, analyzing data, and drawing conclusions.The first step in the scientific inquiry process is making careful observations.Scientists use their senses and scientific tools to gather information about a particular phenomenon or problem.Observations help scientists identify patterns, trends, and relationships in nature.Once observations have been made, scientists ask questions to explore the underlying causes and mechanisms of the phenomenon.These questions lead to the development of hypotheses, which are tentative explanations for the observed phenomena.Hypotheses are based on existing knowledge and are used to guide further investigation.The next step in the scientific inquiry process is conducting experiments.Experiments are designed to test the validity of the hypothesis and to gather data that can be used to support or refute theexplanation.Experiments involve manipulating variables, controlling conditions, and measuring outcomes to determine the effect of one variable on another.After conducting experiments, scientists analyze the data collected to identify patterns, trends, and relationships.They use statistical methods and other analytical tools to interpret the data and draw conclusions about the hypothesis.Conclusions are based on the evidence gathered during the experiment and are used to support or reject the hypothesis.The final step in the scientific inquiry process is communicating the results.Scientists share their findings with the scientific community through publications, presentations, and conferences.This allows other scientists to review, replicate, and build upon the research, which contributes to the overall body of scientific knowledge.In conclusion, the process of scientific inquiry is a systematic approach to understanding natural phenomena.It involves making observations, asking questions, developing hypotheses, conducting experiments, analyzing data, and drawing conclusions.This process is essential for advancing human knowledge and solving problems in various fields, such as medicine, technology, and environmental science.。
常用动物实验设计方法 英语
常用动物实验设计方法英语Common Animal Experiment Design Methods.Animal experiments are a crucial part of scientific research, especially in fields such as biology, pharmacology, and medicine. These experiments aim to understand the functions and mechanisms of living organisms, the effects of various treatments, and the development of new therapeutic strategies. To ensure the validity and reliability of these experiments, it is essential to adopt appropriate experimental design methods. In this article,we will discuss some common animal experiment design methods.1. Controlled Experiments: Controlled experiments are the most basic and fundamental type of animal experiment design. In this design, animals are randomly assigned to different groups, and each group receives a different treatment or condition. By comparing the results between groups, researchers can assess the effects of the treatmentor condition. Controlled experiments aim to minimize confounding variables and ensure that any observed differences are due to the treatment or condition under investigation.2. Randomized Experiments: Randomized experiments are a subtype of controlled experiments where the assignment of animals to different groups is done randomly. This randomization process helps to ensure that any potential differences between groups are distributed evenly, reducing the impact of confounding variables. Randomized experiments are often considered more reliable than non-randomized experiments.3. Blinding: Blinding is a technique used in animal experiments to reduce bias and ensure objectivity. In blinded experiments, the experimenter is unaware of which animals have received which treatment or condition. This lack of knowledge helps to prevent the experimenter from subconsciously favoring one group over another, ensuring that the results are based solely on the treatment or condition under investigation.4. Repeated Measures Design: Repeated measures design involves measuring the same animals multiple times over the course of an experiment. This design allows researchers to assess changes in animals over time and to control for individual differences by comparing within-animal changes. Repeated measures design is commonly used in studies examining the effects of chronic treatments or conditions.5. Factorial Design: Factorial design involves the manipulation of multiple variables simultaneously in an experiment. This design allows researchers to assess the independent and interactive effects of multiple factors on animals. Factorial design is particularly useful in complex experiments where multiple variables may influence the outcome.6. Survival Analysis: Survival analysis is astatistical method used in animal experiments where the primary outcome is the time to a specific event, such as death or the development of a disease. This method allows researchers to compare survival times between differentgroups and to identify factors that influence survival. Survival analysis is commonly used in studies examining the effects of various treatments on animal lifespan or disease progression.In conclusion, the selection of an appropriate animal experiment design method is crucial for ensuring thevalidity and reliability of research findings. The methods discussed in this article, including controlled experiments, randomized experiments, blinding, repeated measures design, factorial design, and survival analysis, are commonly usedin animal experiments and can help researchers to assessthe effects of treatments or conditions on animals. When choosing an experimental design, researchers shouldconsider the specific aims of their study, the nature ofthe treatment or condition under investigation, and the characteristics of the animals used in the experiment. By carefully considering these factors and selecting an appropriate design method, researchers can maximize the scientific value of their animal experiments.。
调查方法有哪些
调查方法有哪些Investigation Methods: What Are They?Investigation methods are the techniques and procedures used to gather information and data for research purposes. These methods are essential to obtaining accurate and reliable results. In this article, we will discuss the different types of investigation methods.1. Surveys。
Surveys are one of the most common investigation methods. They involve asking a set of questions to a group of people to gather data. Surveys can be conducted in person, over the phone, or online. They are often used in market research, social science research, and political polling.2. Interviews。
Interviews are another common investigation method. They involve a one-on-one conversation between the interviewer and the interviewee. Interviews can be structured or unstructured and can be conducted in person or over the phone. They are often used in qualitative research to gather detailed information about a topic.3. Observations。
不容置疑的英语单词
不容置疑的英语单词“不容置疑”可以有以下英语表达:1. undoubted -这是一个形容词,例如:His undoubted talent made him stand out in the competition.(他不容置疑的才华使他在比赛中脱颖而出。
)2. indisputable -同样为形容词,如:It is an indisputable fact that the earth revolves around the sun.(地球绕着太阳转是一个不容置疑的事实。
)3. unquestionable -例如:His loyalty to the company is unquestionable.(他对公司的忠诚是不容置疑的。
)4. incontestable -例如:The evidence presented in the court was incontestable.(法庭上出示的证据是不容置疑的。
)以下不容置疑的英语单词详细分析:一、不容置疑的英语单词-undoubted(一)词源与基本含义“undoubted”源于“doubt”(怀疑)这个单词,加上前缀“un -”表示否定,所以其基本含义就是没有怀疑的,不容置疑的。
它在句子中主要作为形容词来修饰名词。
例如:She has an undoubted gift for music.(她有着不容置疑的音乐天赋。
)(二)用法拓展1. 在描述人的品质方面-可以用来形容一个人的诚实、善良等品质是不容置疑的。
例如:His undoubted integrity has won him many friends.(他不容置疑的正直为他赢得了许多朋友。
)2. 在评价事物的价值时-像一件艺术品的艺术价值,如:The undoubted value of this painting lies in its unique style.(这幅画不容置疑的价值在于它独特的风格。
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Experiments investigation of the effects of surface roughness on laminar flow in macro tubesK.Huang a ,J.W.Wan a ,⇑,C.X.Chen a ,D.F.Mao b ,Y.Q.Li caSchool of Environmental Studies,China University of Geosciences,430074Wuhan,China bWISDRI Engineering &Research Incorporation Limited,Wuhan,China cThree Gorges Survey Research Institute Company Limited,Wuhan,Chinaa r t i c l e i n f o Article history:Received 14September 2012Received in revised form 11October 2012Accepted 14October 2012Available online 27November 2012Keywords:Laminar flowSurface roughness Friction factor Head dropa b s t r a c tThis paper experimentally investigated the effects of surface roughness on laminar flow in macro tubes (d =19mm).Head drop experiments were conducted in six organic glass tubes with relative roughness (D /d )ranging from 1/118.75to 1/6.66.Water head drop data were used to characterize the friction factor over a Reynolds number ranging from 300to 25000.The experimental results for tubes with relative roughness (D /d <5%)were in good agreement with Nikuradse’study.The friction factor could be well predicted by theoretical correlation fRe in laminar flow.Obvious deviation of the friction factor from the classical theory was observed for tubes with large relative roughness (D /d >5%).The product of fRe was larger than 64and increased with an increase in relative roughness.Earlier transition from laminar to turbulent flow was also confirmed.The critical Reynolds number linearly deceased as the relative roughness increased.Ó2012Elsevier Inc.All rights reserved.1.IntroductionThe effects of surface roughness on flow in pipes have been studied over a century.Several of experiments were carried out to investigate the exact effects roughness has on water flow,but whether there were deviation from conventional theory in laminar flow and an early transition from laminar to turbulence flow are still controversial.The pioneering work on effects of surface roughness were stud-ied by Darcy [1]and Fanning [2]in the nineteenth century,how-ever the systematic work on understanding the relationship between surface roughness and pressure drop was conducted by Nikuradse [3].The results of Nikuradse’experiments showed that the value of fRe was equal to 64for laminar flow and the transition Reynolds number from laminar to turbulent flow was around 2000.These were the classical theory on flow in rough pipes and have been used for over a century.In recently years,experimental results in micro scale level have found some new characters in laminar flow.Peiyi and Little [4]are ones of the pioneer investigators who studied the deviation in lam-inar flow in microchannels with high ‘‘relative roughness’’(D /d =0.2–0.3).They studied different gases flow in rectangular and trapezoidal channels (d =55.8–72.4l m).The friction factor ob-tained were larger (10–30%)than those predicted by the classicaltheory and an earlier transition from laminar to turbulent flow oc-curred at Reynolds number from 400to 900.Li [5]experimentally investigated water flow in glass,silicon,and stainless steel micro-tubes with diameters ranging from 80lm to 205l m to studied the effect of surface roughness on flow characteristics.The experimen-tal results showed that the frictional factor measured in smooth tubes made of glass and silicon was in good agreement with the conventional theory.For stainless steel tubes with higher rough-ness,the value of fRe was 15%higher than the theoretical predic-tions in laminar flow when ‘‘relative roughness’’was larger than 5%.An earlier transition also was observed at lower Reynolds num-ber (1700–1900).Tang et al.[6]experimentally investigated flow characteristics for gas in stainless steel and fused silica microtubes with diameter ranging from 50to 200l m.The authors found that the friction fac-tor in silica channels were well predicted by the conventional the-ory while for stainless steel tubes the friction factors were much higher than theoretical values.They explained the phenomenon due to larger relative roughness of stainless steel tubes.When the relative surface roughness was larger than 5%,the friction fac-tor would deviate from the theoretical value.Mala and Li [7]con-ducted experiments on water flow in microtubes with diameters ranging from 50to 254l m.The authors found that for larger diam-eter tubes the experimental results were in good agreement with classic theory,while for smaller tubes the friction factor gradually deviated from the conventional theory and the product fRe in-creased as the diameter of the tubes decrease.The measured fric-0894-1777/$-see front matter Ó2012Elsevier Inc.All rights reserved./10.1016/j.expthermflusci.2012.10.022Corresponding author.E-mail address:cugwanjw@ (J.W.Wan).tion factor may be due to the effects of surface roughness of the microtubes.Xu et al.[8]experimentally investigated waterflow in microchannels with hydraulic diameter ranging from50l m to 300l m.They observed that the experimental results deviated from classical theory for tube diameters lower than100.Judy et al.[9]investigated theflow characteristics of three differentflu-ids(water,hexane,and isopropanol)in capillaries with diameters ranging from20to150l m.The results also revealed that friction factor deviated from conventional theory when the diameter was below100l m.Similar conclusions were given by other investiga-tors[10–12].To investigating the specific effects of relative tough-ness on liquidflow in rough tubes,Kandlikar[13]reassessing Nikuradse’s experiments and proposed a new definition of average roughness[14–16].Brackbill and Kandlikar[17]experimentally determined the effect of roughness and friction factor in micro-tubes.They confirmed that the roughness effect in laminar region and the friction factor could be well predicted by Kandlikar’s con-strictedflow parameters.Schmitt and Kandlikar[18]determined the laminar friction factor by using constricted diameter and ob-tained a correlation between relative roughness and relative roughness.The above results indicated that the relative roughness may be the cause of deviation from conventional theory,but contra-dictory results were gotten by other investigators.Judy et al.[19]conducted experiments on different liquidflow in micro-channels made of fused silica and stainless steel with diameters ranging from15l m to150l m.All experimental data were in good agreement with classic theory and no deviation was ob-served for diameter or material of the tubes.Hwang and Kim [20]investigatedflow characteristics in microtubes with diame-ter of0.244,0.430,and0.792mm.The results could be well pre-dicted by theory within an average deviation of8.9%.The authors considered that the diversity deviation on friction factor may be caused by the inaccuracy measurement of the inner diameter of the tubes.Dutkowski[21]experimentally studied water and airflow in stainless steel tubes with inner diameters of0.55,0.64and 1.1mm.The friction factor obtained from experiments was well predicted by conventional theory.Sara et al.[22]conducted experiments with water in tubes with the diameter of0.2mm and their results showed good agreements with the classic theory.Lorenzini et al.[23]reviewed the past experimental investigations,and then theoretically and experi-mentally studied airflow characteristics in circular channels from26l m to508l m in diameter with different surface rough-ness.The results showed friction factor in laminar regime was in good agreement with the conventional theory.For both smooth and rough tubes.The authors consider that the deviation from the Poiseuille law for microchannel with diameter less than 100l m was due to compressibility effects but not surface roughness effects.Barlak et al.[24]investigated friction factor and pressure drop in tubes from0.2mm to0.589mm in diame-ter and the length-to-diameter ratios ranging from16to265. The experimental data also well agreed with the conventional theory.Although many experimental studies have found large relative roughness may cause the deviation from conventional theory in laminar regime,it’s still a controversial issue and required further investigation.All the above-mentioned experiments were con-ducted in microchannels with different diameters and the surface roughness was only determined by the material of tubes,so the ef-fects of roughness onflow characteristics haven’t been systematic investigated.Nikuradse conducted experiments in tubes with low relative roughness,but deviation in laminarflow was always ob-served in tubes with large relative roughness(D/d>%5).The focus of this paper is to present a comprehensive experimental investiga-tion on pressure drop and friction factor of laminarflow through tubes with relative roughness ranging from1/118.75to1/6.66. The tubes with lower roughness were used to verify the accuracy of Nikuradse’s experiments,and the larger roughness tubes were to explore the relationship between relative roughness andflow characteristics in laminar regime.Further,the experimental results will be compared with the past work.Possible reason for the phe-nomenon of laminarflow in rough tubes will be proposed.Espe-cially,the follow two questions will be discussed:(1)Whether the value of fRe is large than64in rough tubes?(2)Whether there is an earlier transition to turbulentflow inrough tubes?2.Experimental setup and data reduction2.1.Experimental setupThe experimental apparatus used in this investigation is de-picted schematically in Fig.1.It mainly consists of three parts: an adjustment valve,test section(rough tubes)and measurement equipment.Water was supplied by the centrifugal pump to the test tube andflow rate was adjusted by the inflow valve.After passing through the test tube,water was collected by a tank.Thefluidflow rate was calculated by water volume collected over a correspond-ing measured elapsed time.To minimize errors,flow rate were ob-served three times for a certain head drop and mean value was adopted.Three piezometer tubes were used to measure the water head drop.The location of the piezometer tubes was shown in Fig.1.Piezometer tubes were500mm away from both ends of the test tube to decrease the inlet and outlet effects.Meanwhile, water temperature was also measured by thermometer.In this study,the sections included six organic glass tubes with different relative roughness(D/d).Each test tube is3000mm in length,19mm in inner diameter(d).Method to create roughness on the inside walls of the tubes was similar with Nikuradse’exper-iments.To keep uniform roughness throughout the tubes,sifted sand was chosen to create the surface roughness(D).For example, in order to obtain surface roughness of0.16mm,sand was sifted in sieves having opening of0.15–0.17mm diameter.The information of rough tubes with different relative roughness is shown in Table1.But the method to stick the sand grains to the walls of the tubes in this study was different from Nikuradse’experiments. In Nikuradse’experiments,Japanese lacquer was chosen as binder. The tubes werefirstfilled with Japanese lacquer and then emptied, and thenfilled with sifted sand grain and emptied.To assure good adherence of sand grains to the wall,the tubes were refilled with lacquer and again emptied after a drying period of2–3weeks.This resulted in a thin coat of lacquer on the surface of the particles which caused the relative roughness values ranged between0% and3.3%[13].Therefore,Chloroform was used to stick the sand grains to the walls of the tubes and the refilled process was not adapted in this study.2.2.Data reductionReynolds number(Re)and friction factor(f),were defined as the same with Nikuradse.Reynolds number was calculated from equa-tion with the following form:Re¼udmð1Þin which m is the kinematic viscosity,u is the averageflow velocity which can be determined by the following formula:u¼QÁ4p dð2Þ244K.Huang et al./Experimental Thermal and Fluid Science45(2013)243–248where Q is the measured flow rate,d is the diameter of the tube.Once the average flow velocity has been determined from Eq.(2),Reynolds number could be obtained by putting u in Eq.(1).The friction factor f is determined as the following equation:f ¼2gdu 2J ð3ÞWhere J is the hydraulic gradient which means the head drop re-ferred to a length unit of the tube.The experimental uncertainties of the parameters were listed in Table 2.The uncertainty of the Reynolds number and the friction factor is determined by equation using the method described by Abernethy et al.[25].y ¼f ðx 1;x 2;...;x N Þd y ¼ffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi@f @x 1d x 1 2þÁÁÁþ@f@x Nd x N 2"#v u u tð4ÞBy applying Eq.(4),the calculated mean uncertainty for theReynolds number and friction factor are 3.2%and 5.9%,respectively.3.Results and discussionWater head drop was measured with the tubes had different surface roughness at various flow rates.Reynolds number is calcu-lated by Eq.(1).Fig.2shows the experimental results of hydraulicgradient (J )depending of Reynolds number for water flow in six rough tubes with relative roughness D /d =1/118.75,1/61.79,1/30.89,1/14.18,1/10.56,1/6.66.Fig.2a presents that the value ofJTable 1Relative roughness results.Number d (mm)D (mm)Sieves diameter (mm)D /d R1190.160.15–0.171/118.75R20.30750.3–0.3151/61.79R30.6150.6–0.631/30.89R4 1.34 1.25–1.431/14.18R5 1.8 1.6–21/10.56R62.852.5–3.21/6.66Table 2Experimental uncertainties of the major parameters.Parameter Uncertainty ParameterUncertainty Diameter (d )±1%Time (t )±0.1s Temperature (T )±0.1°C Volume (V )±1%Head (H )±1mm Reynolds number (Re )±3.2%Length (L )±1mmFriction factor (f )±5.9%K.Huang et al./Experimental Thermal and Fluid Science 45(2013)243–248245increased as the value of Re increased.With the same value of Re, the hydraulic gradient value was greater for tubes having larger relative roughness.On the transition from laminarflow into turbu-lentflow,the relationship between Re and J shows a non-linear behavior.Fig.2b shows the slope of the curve on J versus Re rise rapidly at Re=2000for tubes R1-4.However,it seems that the transition for tubes R5-6occurred a little earlier for Re in the range of1500–1800(Fig.2c).Besides,the hydraulic gradient values of R1 are sometimes slightly higher than the hydraulic gradient values of R2when Re is larger than about2100(Fig.2b).This phenomenon occurred in the region called thefirst portion of turbulentflow by Nikuradse,where the waterflow became unstable with the streamlinefluctuating stochastically.Considering that the value of J was close in this region,theflowfluctuation may influence the measurement of water head drop and the experiments results.In order to ascertain whether the experimental results deviated from classical theory for tubes with large surface roughness,theMoody chart was used to plot the experimental data.The theoret-ical value of fRe is equal to64for incompressible laminarflow in circular tube.In Fig.3,friction factor is plotted as a function of Re by experi-mental data from both Nikuradse’study with relative roughness D/ d=1/120,1/60,1/30and this study with D/d=1/118.75,1/61.79, 1/30.89.Fig.3shows the results of experimental investigations were in good agreement with Nikuradse’in laminarflow.It can be noted that the experimental data follow with the theoretical lin-ear law at low Reynolds number within the range of 300<Re<2000.The roughness had no effects on the friction in laminarflow and the value of friction factor is equal to64/Re. The deviation from laminar to turbulentflow occurred around Re=2000.However,the friction factors of this research were high-er than the friction factors of Nikuradse’research when Re is larger than about6000,although the relative roughness is almost the same(1/30.89and1/30,1/61.79and1/60,1/118.75and1/120). This could be explained by the different method for sticking the sand grains.The process that refilled lacquer in the test tubes by Nikuradse would result in the relative roughness less than actual value which caused the friction factors decreasing as well in tran-sitional and turbulent region.Fig.4presents the friction factor as a function of Reynolds num-ber for tubes with larger value of relative roughness(D/d=1/14.18, 1/10.56,1/6.66).In the laminar region,the diagram still shows lin-ear relationship between friction factor and Reynolds number,but the product of fRe is no longer equal to64.The value of friction fac-tor increased with an increase in relative roughness for the same value of Reynolds number.In attempt to investigate the roughness effect on friction factor, product of fRe is plotted as a function of D/d by experimental results(Fig.5).Fig.5shows that the value of fRe slowly rose as the relative roughness increased for laminarflow when the relative roughness is less than1/30.89and the value of fRe increase qua-dratic as the Re increased when the relative roughness is larger than5%.The correlation of experimental data gave the following equation with R2=0.9985.It can be seen that surface roughness caused a sharp increase of fRe for laminarflow when the relative roughness is larger than5%.Similar conclusion was also obtained by Kandlikar et al.[26],Brackbill and Kandlikar[17].Constricted flow was used to explain the deviation from theoretical law in lam-inarflow in tubes with large surface roughness in Kandlikar’s study.The constrictedflow parameter is defined as follows:d cf¼dÀ2Dð5Þwhere d cf is the constrictedflow diameter,d is the inner diameter of the tube,and D is the surface roughness.The Reynolds number and friction factor were accordingly defined based on the constricted flow diameter as follows:Re cf¼u cf d cfm¼dcfÁReð6Þf cf¼2gdcfu2ÁJ¼fÁd5cfdð7ÞThe product of fRe can be written as Eq.(8):fRe¼f cf Re cfÁd4d4cf¼f cf Re cfÁd4ðdÀ2DÞ4ð8ÞThe value of f cf Re cf in laminarflow is equal to64,so Eq.(8)can be written as follows:246K.Huang et al./Experimental Thermal and Fluid Science45(2013)243–248fRe¼64Ád4ðdÀ2DÞ4ð9ÞEq.(9)indicated that the value of fRe is equal to64in laminar flow region for smooth tubes(D=0).As the surface roughness in-creased,the product of fRe increased accordingly.When the surface roughness is small enough(D/d<5%),the deviation from laminar flow can be neglected.The above analysis has been verified by the experimental results.It can be seen from Fig.4that the effect of roughness on transi-tion to turbulence was also confirmed by experimental results.The critical Reynolds number Re c decreased with an increase of surface roughness,for the increased roughness would provide larger inter-acted area on the surface roughness.The surface roughness would bend theflow line near the inner surface,causing earlier transition to turbulence.The critical Reynolds number was plotted as a func-tion of relative roughness in Fig.6.As what mentioned before,the critical Reynolds number Re c for tubes with relative roughness less than1/30was well predicted by the theoretical law,with the value of Re c around2000.But for tubes with relative roughness larger than0.06,the critical Rey-nolds number dramatically decreased.The critical Reynolds num-ber has been modeled as a linefit and is given as Re c=2277.7À5965(D/d)with a coefficient of determination(R2) of0.9986.Brackbill and Kandlikar proposed similar linear correla-tion to predict the critical Reynolds number forflow in microchan-nel,which agreed with constricted model well[27].But the slope of the correlation presented by Brackbill and Kandlikar is higher than that in this study.The correlation to predict the critical Rey-nolds number forflow in macro tubes should be investigated by more experiments.4.ConclusionsThe paper presents experimental results of effect of roughness on waterflow in macro tubes with inner diameter d=19mm. The test tubes were made in organic glass with relative roughness ranging from1/118.75to1/6.66.The main conclusions can be summarized as follows:–The results of experimental investigations were in good agree-ment with Nikuradse’in laminarflow for tubes with D/d<1/ 30;transition from laminarflow to turbulentflow occurred around Re=2000and the product of fRe was equal to64.–Constrictedflow model could explain the deviation from theo-retical law in laminarflow in tubes with large surface roughness and experimental results in this paper also agreed with the model.–Earlier transition was observed when relative roughness D/d>5%;the critical Reynolds number could be calculated byequation:Re c¼2277:7À5965ðD=dÞ.–Friction factor of laminarflow deviated from theoretical corre-lation f Re when relative roughness D/d>5%;the product of fRe could be predicted by equation when relative roughness D/d>5%:fRe=10217(D/d)2À888.86(D/d)+83.454. 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