血液温度对白细胞过滤效果的影响

血液温度对白细胞过滤效果的影响
郑东;张健
【期刊名称】《临床输血与检验》
【年(卷),期】2016(18)6
【摘要】目的：了解不同血液温度对白细胞过滤的影响。

方法使用一次性去白细
胞滤器血袋采集抗凝全血，按过滤前血液检测温度的高低分成3组，第1组血液
温度8℃±2℃，采集后放置2℃～6℃冰箱3 h以上取出后立即过滤；第2组血液温度14℃±2℃，为血液从2℃～6℃冰箱取出后放置室温（24℃）30 min后过滤；第3组血液温度24℃±2℃，血液采集后一直放置室温4 h以上过滤。

测定各组过滤前后白细胞数、过滤时间和过滤后的红细胞膜渗透脆性，并进行对比分析。

结果各组滤过时间的差异有统计学意义（P＜0.05），剩余白细胞数的差异有统计学意义（P＜0.05），红细胞膜脆性试验于2℃～6℃冰箱冷藏3 h以上组与其他两组
的差异均有统计学意义（P＜0.05）。

结论血液温度低，白细胞滤过效果好，但红细胞损伤大；血液温度高，红细胞损伤小，但白细胞滤过效果差；将血液温度维持在14℃±2℃范围，有利于提高血液滤过效果。

%ObjectiveTo investigate the influence of different stored blood temperature on the harvest of leukocyte filtration and the fragility of erythrocyte membrane.Methods The leukocyte filter blood bags were used g to collect anticoagulant blood under various blood temperature.The blood was divided into3 groups before filtration. The temperature of blood of the first group was8±2℃.The blood was placed in2-6℃for more than3h,followed by immediate filtration. The blood temperature of the second group was14±2℃when it was placed
at room temperature(RT,24℃)for30 minbefore filtration. The temperature of blood was24±2℃,which was placed at RT for more than4h before filtration. White blood cell counts were determined in each group before and after filtration. Filtration time,erythrocyte osmotic fragility after filtration were determined and analysed.Result The time and white blood cell counts after filtration showed significant differences(P<0.05),the test results of erythrocyte osmotic fragility were statistically significant between the first group and the other two groups(P<0.05).Conclusion The recovery of leukocyte filtration and the erythrocyte membrane injuries increased and with the decreased blood temperature. The appropriate temperature of blood storage is 14±2℃.
【总页数】3页(P537-538,539)
【作者】郑东;张健
【作者单位】222001 江苏省连云港市中心血站;222001 江苏省连云港市中心血站【正文语种】中文
【中图分类】R331.1+42
【相关文献】
1.怡开对白细胞粘附和血液流变性影响的研究 [J], 刘育英;赵秀梅;刘凤英;郑世荣;王耀芳;佟宪
2.血液透析联合血液灌流治疗糖尿病肾病患者对白细胞介素-6和C-反应蛋白的影响及意义分析 [J], 钟姝涵
3.维持性血液透析患者外周血单个核细胞NF-κB活性水平观察及其对白介素-6表
达的影响 [J], 许元文;李晓艳;张涤华;沈清瑞;李幼姬;余学清
4.血液病患者外周血有核红细胞对白细胞计数的影响 [J], 张婉琳;崔碧云;邹志杰;黄飞龙
5.环孢素治疗难治性自身免疫性血液病的效果及对白细胞介素17A、白细胞介素21水平的影响 [J], LI Qi;FAN Ling;TONG Changqing;LI Yajun
因版权原因，仅展示原文概要，查看原文内容请购买。