Simultaneous velocimetry and thermometry of air by use of nonresonant heterodyned laser-ind
热定形凝胶溶胶凝胶转变温度乙二醇柠檬酸中英文资料外文翻译
中英文资料外文翻译可逆热固性原位胶凝流变特性的解决方案与甲基纤维素聚乙二醇柠檬酸三元系统Masanobu Takeuchi Shinji Kageyama Hidekazu Suzuki Takahiro著,…..译.[摘要]可逆性溶胶凝胶温度的转变受到甲基纤维素(MC)、聚乙二醇(PEG)、柠檬酸(SC) 三元体系的影响,通过流变学测量得出原位凝胶体系的性能。
当PEG (4000)的浓度在0%到10%范围内变化,MC(25)和SC浓度分别保持在1.5%和3.5%时,随着PEG浓度的增加,可逆性溶胶转变温度从38°C降低至26°C,然而,温度降低的程度不受PEG分子量的影响,随着MC浓度的增加可逆溶胶~凝胶的温度降低,同时随着ph值的降低可逆溶胶~凝胶的温度升高,在流变特性的比较方面,目前原位胶凝的设置解决方案和常规相比,如结冷胶溶液或泊洛沙姆407,显示目前的解决方案从根本上有别于传统的解决方案,这些研究结果表明,这项研究中的三元体系可作为在眼部传递灌输系统的药物。
[关键词] 热定形凝胶;溶胶凝胶转变温度;甲基纤维素聚乙二醇柠檬酸三元体系1 前言本研究提高了眼用溶液在吸收过程中利用度差的问题,例如,在溶液溶解时利用这个属性而由此获得的聚合物。
通过在滴眼液中加入聚合物来延长持续时间,从而增加药物在角膜前停留时间来改善结膜渗透性。
聚合物的使用被认为是有效的,因为他们增加了药物的效用,聚合物的使用也有其缺点,如由于溶液的粘度高会出现灌注困难和不适感。
我们发现了一种热固性凝胶溶液在甲基纤维素聚乙二醇柠檬酸三元系统中的应用,并开发了一种含马来酸噻吗洛尔,可以用来治疗青光眼的眼用溶液,据报道,长效的眼用溶液的流量曲线触变性在32°C,呈现出粘度随温度升高而明显变化的特性。
据报道,眼科溶液流变特性极大地影响角膜滞留时间和眼睛的感觉,我们考察了不同聚合物溶液的性质,以前几乎没有从流变学的观点来研究的先例。
顶空固相微萃取-气相质谱联用英文表达
顶空固相微萃取-气相质谱联用英文表达全文共6篇示例,供读者参考篇1Headspace Solid Phase Microextraction-Gas Chromatography Mass SpectrometryHey kids! Have you ever wondered how scientists can identify different chemicals and understand what things are made of? Well, today we're going to learn about a really cool technique called "Headspace Solid Phase Microextraction-Gas Chromatography Mass Spectrometry" – that's a mouthful, isn't it? Let's just call it HS-SPME-GCMS for short.HS-SPME-GCMS is like a super detective tool that helps scientists figure out the different ingredients or chemicals present in all sorts of samples, from foods and beverages to environmental samples and even human breath! It's kind of like a high-tech bloodhound that can sniff out and identify the tiniest traces of chemicals.So, how does it work? Well, it's actually a combination of three different techniques working together like awell-coordinated team. Let's break it down:Headspace Solid Phase Microextraction (HS-SPME)This is the first step in our detective process. Imagine you have a bottle of soda or a jar of pickles, and you want to know what kinds of chemicals are giving them their unique smells and flavors. That's where HS-SPME comes in!It uses a tiny fiber (smaller than a strand of hair!) coated with a special material that can absorb and trap the chemicals that are vaporizing or evaporating from the sample. This is called the "headspace" – the air or gas above the sample. By exposing the fiber to the headspace, it collects a concentrated sample of the chemicals present.Gas Chromatography (GC)After the HS-SPME fiber has collected the chemicals from the headspace, it's time to separate and analyze them individually. That's where the Gas Chromatograph (GC) comes in.The GC is like a long, thin tube filled with a special material that acts like a maze for the chemicals. As the chemicals travel through the tube, they get separated based on their properties, like how quickly they move or how strongly they interact with the tube's material.It's kind of like a foot race, where some chemicals move faster than others, and they all finish at different times. This separation allows the scientists to identify each chemical individually.Mass Spectrometry (MS)But how do the scientists actually figure out what each chemical is? That's where the Mass Spectrometer (MS) comes in – it's like a super-advanced scale that weighs and identifies the chemicals.As the separated chemicals exit the GC, they enter the MS, where they are bombarded with electrons that break them apart into smaller pieces called ions. These ions are then measured and analyzed based on their mass and unique patterns, which are like fingerprints for each chemical.By comparing these patterns to a huge database of known chemicals, the MS can identify what each chemical is with incredible accuracy. It's like matching the fingerprints to a giant collection of mugshots!So, there you have it – HS-SPME-GCMS is a powerful combination of techniques that allows scientists to extract, separate, and identify even the smallest traces of chemicals in allsorts of samples. It's like having a team of super-detectives working together to solve the mystery of what's really in the things around us.Pretty cool, right? Who knows, maybe one day you'll be using HS-SPME-GCMS to analyze the ingredients in your favorite snacks or to help solve environmental mysteries! The possibilities are endless when you have the right tools and a curious mind.篇2Smelly Science: Exploring Scents with HS-SPME-GCMSHave you ever wondered how scientists can identify different smells? Well, get ready to dive into the fascinating world of Headspace Solid-Phase Microextraction-Gas Chromatography Mass Spectrometry (HS-SPME-GCMS)! It's a mouthful, but it's also an incredibly cool way to study the tiny molecules that create all sorts of smells.Let's break it down, shall we?Headspace: Imagine a small, sealed container with a little bit of air inside. That's the "headspace" we're talking about. It's like a tiny room where smelly molecules can hang out.Solid-Phase Microextraction: This fancy term refers to a special tool that scientists use to capture those smelly molecules from the headspace. It's a tiny fiber, coated with a special material that acts like a magnet for smells. When the fiber is exposed to the headspace, it attracts and traps the smelly molecules on its surface.Gas Chromatography: After the fiber has collected all the smelly molecules, it's time to separate them. That's where gas chromatography comes in. It's like a long, winding road that the molecules travel through. Depending on their size and shape, some molecules move faster than others, allowing scientists to tell them apart.Mass Spectrometry: As the separated molecules reach the end of the road, they encounter the mass spectrometer. This clever machine breaks the molecules into even smaller pieces called ions and measures their weights. Each molecule has a unique weight pattern, which acts like a fingerprint that scientists can use to identify it.Putting it all together, HS-SPME-GCMS is like asuper-powered nose that can sniff out and identify even the tiniest smells in a sample. Pretty cool, right?But why would scientists want to do this? Well,HS-SPME-GCMS has all sorts of awesome applications!Forensics: Imagine a detective using HS-SPME-GCMS to analyze the smells left behind at a crime scene. By identifying the molecules present, they might be able to figure out what kind of materials or substances were involved, helping them solve the case!Food Science: Have you ever wondered what makes your favorite snack smell so delicious? Food scientists useHS-SPME-GCMS to study the aroma compounds in different foods, which helps them create new flavors or improve existing ones.Environmental Monitoring: HS-SPME-GCMS can also be used to detect and identify pollutants in the air or water. By analyzing the molecules present, scientists can better understand the sources of pollution and work on ways to reduce it.Medicine: Believe it or not, some diseases can cause changes in the way our bodies smell. HS-SPME-GCMS can help doctors detect these changes and potentially use them as diagnostic tools.So, the next time you catch a whiff of something delicious or strange, remember the incredible science of HS-SPME-GCMS!It's like having a super-powered nose that can unravel the mysteries of all sorts of smells, one molecule at a time.篇3Title: Sniffing Out Smells with a Super SnifferHave you ever wondered how scientists can identify different smells in the air? Well, they use a powerful tool called Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry, or HS-SPME-GC-MS for short. It's a bit of a mouthful, isn't it? But don't worry; I'll explain it in a way that's easy to understand.Imagine you're at a bakery, and you can smell the delicious aroma of freshly baked bread, cakes, and pastries. That's because tiny particles called molecules are floating in the air, and your nose can detect them. But how do scientists know exactly which molecules are responsible for each smell?That's where the HS-SPME-GC-MS comes in. It's like a super sniffer that can identify and separate all the different smells in the air. Let's break it down:Headspace Solid-Phase Microextraction (HS-SPME)This part of the tool is like a tiny fishing rod that can catch molecules from the air. It has a special coating that attracts and holds onto the molecules we want to study. Imagine it's like a magnet for smells!Gas Chromatography (GC)After the HS-SPME has caught the molecules, they need to be separated so we can study them one by one. That's where the Gas Chromatography part comes in. It's like a long, twisty tunnel that the molecules travel through. Depending on their size and shape, some molecules move faster than others, so they separate from each other along the way.Mass Spectrometry (MS)Once the molecules are separated, they need to be identified. That's where the Mass Spectrometry part comes in. It's like a molecule weighing station. Each molecule is weighed and given a special code based on its weight and structure. Scientists can then use this code to figure out exactly what kind of molecule it is.So, let's imagine you're at the bakery again. The HS-SPME would catch all the different smells in the air, like the sweetaroma of vanilla, the buttery smell of croissants, and the yeasty scent of bread. The GC would separate these smells into individual molecules, and the MS would identify each one by weighing and coding them.Pretty cool, right? Scientists use this super sniffer to study all kinds of smells, from the fragrance of flowers to the odors produced by chemical spills or even explosives. It helps them understand the world around us and keep us safe.Next time you catch a whiff of something delicious or interesting, remember the HS-SPME-GC-MS and how it helps scientists sniff out all the different smells in the air. Who knows, maybe you'll become a scientist one day and get to use this amazing tool yourself!篇4Sniffing Out the Secrets: A Cool Way to Detect SmellsHey there, kids! Have you ever wondered how scientists can identify all sorts of different smells in the air? Well, let me tell you about a really cool technique called "Headspace Solid-Phase Microextraction coupled with Gas Chromatography-Mass Spectrometry" – that's a mouthful, isn't it? Let's just call it "the smelly smell detector" for short.Imagine you're at a bakery, and you can smell the delicious aroma of freshly baked bread. That wonderful smell is made up of tiny particles called "volatile organic compounds" or VOCs for short. These VOCs are what give things their distinct smells, whether it's the sweet scent of cookies or the stinky odor of rotten eggs.Now, scientists have a really cool way to capture and identify these VOCs using the smelly smell detector. It's like a tiny vacuum cleaner that sucks up the smelly particles from the air or from a sample.Here's how it works:First, there's a tiny fiber made of special materials like silica or polymers. This fiber acts like a magnet for VOCs, attracting and trapping them onto its surface. It's like a sticky trap for smelly particles!Next, the fiber is exposed to the air or sample that needs to be analyzed. The VOCs in the air or sample get stuck onto the fiber, kind of like how a magnet attracts metal objects.After the fiber has collected enough VOCs, it's time to analyze them using the Gas Chromatography-Mass Spectrometry part of the smelly smell detector.Gas Chromatography is like a race track for the VOCs. The trapped VOCs are released from the fiber and sent through a long, thin tube called a "column." Inside this column, there's a special coating that makes different VOCs move at different speeds, just like how some runners are faster than others.As the VOCs exit the column one by one, they enter the Mass Spectrometer, which is like a super-advanced weighing machine. It breaks each VOC into tiny pieces called "ions" and measures their weight and amounts. This information is used to identify the specific VOCs present in the sample.Pretty cool, right? It's like having a secret code-breaker that can tell you exactly what smells are in the air or in a sample.Scientists use the smelly smell detector for all sorts of things. They can analyze the aromas of foods, wines, and perfumes to understand what makes them smell so good (or bad!). They can also detect environmental pollutants or identify the presence of drugs or explosives by their unique VOC signatures.Imagine being able to sniff out a hidden stash of cookies just by analyzing the air with the smelly smell detector! Or maybe you could use it to figure out who left that stinky surprise in the classroom (hopefully not!).So, the next time you catch a whiff of something delicious or stinky, remember that there's a whole world of tiny VOCs floating around, just waiting to be detected by the amazing smelly smell detector!篇5The Amazing Science Adventure: Top-Space Solid-Phase Microextraction-Gas Chromatography Mass SpectrometryHello, my curious friends! Today, I want to take you on an exciting journey into the world of science. Are you ready? Let's go!Have you ever wondered how scientists can find tiny traces of things that we can't even see? Well, they have a super cool method called "Top-Space Solid-Phase Microextraction-Gas Chromatography Mass Spectrometry." Wow, that's a mouthful! Let's break it down and learn about each part.First, there's "Top-Space." It's like a magic trick where scientists capture invisible things from the air. They use a special device that can trap tiny particles floating around. These particles might be pollutants, flavors, or even scents from your favorite foods!Next, we have "Solid-Phase Microextraction." This is where the real science magic happens. Scientists use a tiny needle-like thing called a fiber to absorb those invisible particles we talked about earlier. It's like a super sponge that soaks up all the interesting stuff. The fiber acts like a detective, collecting clues from the air.Now, let's move on to "Gas Chromatography." It's like a special sorting machine that helps scientists separate all the different particles they collected. Just imagine a train station where each train represents a different particle. The sorting machine makes sure each particle gets on the right train. This way, scientists can figure out what kinds of particles they have.Last but not least, we have "Mass Spectrometry." This part is like having a superpower to identify the particles. Scientists use a special machine to "weigh" the particles and figure out what they're made of. It's like having a fingerprint scanner for particles! This helps scientists understand if the particles are good or bad, and how they might affect our world.Now, you might be wondering why all of this is important. Well, my friends, scientists use this cool technique to learn more about our environment. They can find out if there are harmfulpollutants in the air, water, or even in the food we eat. This helps them keep us safe and make our world a better place.So, next time you see scientists in their lab coats, remember that they are like superheroes using their powers to protect us. They use "Top-Space Solid-Phase Microextraction-Gas Chromatography Mass Spectrometry" to solve mysteries and make amazing discoveries.Science is truly an incredible adventure, my friends. I hope you enjoyed this journey into the world of "Top-SpaceSolid-Phase Microextraction-Gas Chromatography Mass Spectrometry." Keep asking questions and exploring the wonders of science!Remember, the world is full of amazing things waiting to be discovered. Who knows, maybe one day it will be you, my little scientists, who will make the next big discovery using"Top-Space Solid-Phase Microextraction-Gas Chromatography Mass Spectrometry"!Happy exploring!篇6Smelling the World with Science!Have you ever noticed how different things have their own special smells? The sweet aroma of freshly baked cookies, the earthy scent of a garden after rain, or the stinky odor of a trash can - smells are all around us! But did you know that scientists can actually study and identify those smells using some really cool techniques?One amazing way they do this is called "HeadspaceSolid-Phase Microextraction - Gas Chromatography Mass Spectrometry." That's a huge mouthful, right? Let's break it down!Headspace is the air or space above a solid or liquid sample, like the air above a cup of hot chocolate. All those yummy chocolate smells are in the headspace! Solid-Phase means the stuff that traps and holds onto the smells is a solid material. Microextraction means they only need a very tiny amount of the trapped smells to study them.So Headspace Solid-Phase Microextraction (let's just call it HSSPM for short) is a way to capture and concentrate the smells from the headspace of a sample onto a solid material. But how do we figure out what those captured smells actually are? That's where the Gas Chromatography Mass Spectrometry part comes in!Gas Chromatography is like a huge nose that can sniff out and separate all the different smells in a mixture. It does this by vaporizing the sample (turning it into a gas) and then pushing that gas through a long, thin tube called a column. Different smells move through the column at different speeds, so they get separated from each other.At the end of the column, the separated smells get analyzed by a Mass Spectrometer. This amazing machine uses magnetism to weigh and identify each individual smell based on its unique pattern. It's like having a device that can recognize every single person just by their smell signature!Together, Gas Chromatography and Mass Spectrometry (let's call it GC-MS) can tell us exactly what kinds of smells were present in the original sample and how much of each smell there was. When you combine HSSPM with GC-MS, you get a powerful tool that can sniff out and identify even the faintest of odors from almost any kind of sample - food, drinks, plants, chemicals, you name it!But why would scientists want to study smells so carefully? Well, there are lots of reasons! Food scientists can use it to figure out what gives certain foods their delicious flavors. Environmental scientists can check for pollutants or track downthe source of weird smells. Forensic scientists can analyze smell evidence from crime scenes. And perfume makers can study the smells of different flowers and ingredients to create new, amazing fragrances.Isn't it amazing how we can use science and technology to explore the world of smells all around us? From a single whiff, we can uncover so much information and solve all kinds of mysteries and problems. So next time you take a deep breath and smell something interesting, remember - you're experiencing chemistry in action! Who knows, you might even grow up to be a smell scientist yourself one day!。
马蹄涡与壁面传热 (2)
GT2005-69091
GT2005-69091
THE DYNAMICS OF THE HORSESHOE VORTEX AND ASSOCIATED ENDWALL HEAT TRANSFER, PART 2 – TIME-MEAN RESULTS
T. J. Praisner Turbine Aerodynamics and Durability United Technologies Pratt & Whitney 400 Main St., M/S 169-29 East Hartford, CT 06108 ABSTRACT Time-mean endwall heat transfer and flow-field data in the endwall region are presented for a turbulent juncture flow formed with a symmetric bluff body. The experimental technique employed allowed the simultaneous recording of instantaneous particle image velocimetry flow field data, and thermochromic liquid-crystal-based endwall heat transfer data. The time-mean flow field on the symmetry plane is characterized by the presence of primary (horseshoe), secondary, tertiary, and corner vortices. On the symmetry plane
单分子综述-NATURE NANOTECHNOLOGY-Single-molecule junctions beyond electronic transport-2013
Stimulated by the initial proposal that molecules could be used as the functional building blocks in electronic devices 1, researchers around the world have been probing transport phenomena at the single-molecule level both experimentally and theoretically 2–11. Recent experimental advances include the demonstration of conductance switching 12–16, rectification 17–21, and illustrations on how quantum interference effects 22–26 play a critical role in the electronic properties of single metal–molecule–metal junctions. The focus of these experiments has been to both provide a fundamental understanding of transport phenomena in nanoscale devices as well as to demonstrate the engineering of functionality from rational chemical design in single-molecule junctions. Although so far there are no ‘molecular electronics’ devices manufactured commercially, basic research in this area has advanced significantly. Specifically, the drive to create functional molecular devices has pushed the frontiers of both measurement capabilities and our fundamental understanding of varied physi-cal phenomena at the single-molecule level, including mechan-ics, thermoelectrics, optoelectronics and spintronics in addition to electronic transport characterizations. Metal–molecule–metal junctions thus represent a powerful template for understanding and controlling these physical and chemical properties at the atomic- and molecular-length scales. I n this realm, molecular devices have atomically defined precision that is beyond what is achievable at present with quantum dots. Combined with the vast toolkit afforded by rational molecular design 27, these techniques hold a significant promise towards the development of actual devices that can transduce a variety of physical stimuli, beyond their proposed utility as electronic elements 28.n this Review we discuss recent measurements of physi-cal properties of single metal–molecule–metal junctions that go beyond electronic transport characterizations (Fig. 1). We present insights into experimental investigations of single-molecule junc-tions under different stimuli: mechanical force, optical illumina-tion and thermal gradients. We then review recent progress in spin- and quantum interference-based phenomena in molecular devices. I n what follows, we discuss the emerging experimentalSingle-molecule junctions beyond electronic transportSriharsha V. Aradhya and Latha Venkataraman*The id ea of using ind ivid ual molecules as active electronic components provid ed the impetus to d evelop a variety of experimental platforms to probe their electronic transport properties. Among these, single-molecule junctions in a metal–molecule–metal motif have contributed significantly to our fundamental understanding of the principles required to realize molecular-scale electronic components from resistive wires to reversible switches. The success of these techniques and the growing interest of other disciplines in single-molecule-level characterization are prompting new approaches to investigate metal–molecule–metal junctions with multiple probes. Going beyond electronic transport characterization, these new studies are highlighting both the fundamental and applied aspects of mechanical, optical and thermoelectric properties at the atomic and molecular scales. Furthermore, experimental demonstrations of quantum interference and manipulation of electronic and nuclear spins in single-molecule circuits are heralding new device concepts with no classical analogues. In this Review, we present the emerging methods being used to interrogate multiple properties in single molecule-based devices, detail how these measurements have advanced our understanding of the structure–function relationships in molecular junctions, and discuss the potential for future research and applications.methods, focusing on the scientific significance of investigations enabled by these methods, and their potential for future scientific and technological progress. The details and comparisons of the dif-ferent experimental platforms used for electronic transport char-acterization of single-molecule junctions can be found in ref. 29. Together, these varied investigations underscore the importance of single-molecule junctions in current and future research aimed at understanding and controlling a variety of physical interactions at the atomic- and molecular-length scale.Structure–function correlations using mechanicsMeasurements of electronic properties of nanoscale and molecu-lar junctions do not, in general, provide direct structural informa-tion about the junction. Direct imaging with atomic resolution as demonstrated by Ohnishi et al.30 for monoatomic Au wires can be used to correlate structure with electronic properties, however this has not proved feasible for investigating metal–molecule–metal junctions in which carbon-based organic molecules are used. Simultaneous mechanical and electronic measurements provide an alternate method to address questions relating to the struc-ture of atomic-size junctions 31. Specifically, the measurements of forces across single metal–molecule–metal junctions and of metal point contacts provide independent mechanical information, which can be used to: (1) relate junction structure to conduct-ance, (2) quantify bonding at the molecular scale, and (3) provide a mechanical ‘knob’ that can be used to control transport through nanoscale devices. The first simultaneous measurements of force and conductance in nanoscale junctions were carried out for Au point contacts by Rubio et al.32, where it was shown that the force data was unambiguously correlated to the quantized changes in conductance. Using a conducting atomic force microscope (AFM) set-up, Tao and coworkers 33 demonstrated simultaneous force and conductance measurements on Au metal–molecule–metal junc-tions; these experiments were performed at room temperature in a solution of molecules, analogous to the scanning tunnelling microscope (STM)-based break-junction scheme 8 that has now been widely adopted to perform conductance measurements.Department of Applied Physics and Applied Mathematics, Columbia University, New York, New York 10027, USA. *e-mail: lv2117@DOI: 10.1038/NNANO.2013.91These initial experiments relied on the so-called static mode of AFM-based force spectroscopy, where the force on the canti-lever is monitored as a function of junction elongation. I n this method the deflection of the AFM cantilever is directly related to the force on the junction by Hooke’s law (force = cantilever stiff-ness × cantilever deflection). Concurrently, advances in dynamic force spectroscopy — particularly the introduction of the ‘q-Plus’ configuration 34 that utilizes a very stiff tuning fork as a force sen-sor — are enabling high-resolution measurements of atomic-size junctions. In this technique, the frequency shift of an AFM cantilever under forced near-resonance oscillation is measuredas a function of junction elongation. This frequency shift can be related to the gradient of the tip–sample force. The underlying advantage of this approach is that frequency-domain measure-ments of high-Q resonators is significantly easier to carry out with high precision. However, in contrast to the static mode, recover-ing the junction force from frequency shifts — especially in the presence of dissipation and dynamic structural changes during junction elongation experiments — is non-trivial and a detailed understanding remains to be developed 35.The most basic information that can be determined throughsimultaneous measurement of force and conductance in metalThermoelectricsSpintronics andMechanicsOptoelectronicsHotColdFigure 1 | Probing multiple properties of single-molecule junctions. phenomena in addition to demonstrations of quantum mechanical spin- and interference-dependent transport concepts for which there are no analogues in conventional electronics.contacts is the relation between the measured current and force. An experimental study by Ternes et al.36 attempted to resolve a long-standing theoretical prediction 37 that indicated that both the tunnelling current and force between two atomic-scale metal contacts scale similarly with distance (recently revisited by Jelinek et al.38). Using the dynamic force microscopy technique, Ternes et al. effectively probed the interplay between short-range forces and conductance under ultrahigh-vacuum conditions at liquid helium temperatures. As illustrated in Fig. 2a, the tunnel-ling current through the gap between the metallic AFM probe and the substrate, and the force on the cantilever were recorded, and both were found to decay exponentially with increasing distance with nearly the same decay constant. Although an exponential decay in current with distance is easily explained by considering an orbital overlap of the tip and sample wavefunctions through a tunnel barrier using Simmons’ model 39, the exponential decay in the short-range forces indicated that perhaps the same orbital controlled the interatomic short-range forces (Fig. 2b).Using such dynamic force microscopy techniques, research-ers have also studied, under ultrahigh-vacuum conditions, forces and conductance across junctions with diatomic adsorbates such as CO (refs 40,41) and more recently with fullerenes 42, address-ing the interplay between electronic transport, binding ener-getics and structural evolution. I n one such experiment, Tautz and coworkers 43 have demonstrated simultaneous conduct-ance and stiffness measurements during the lifting of a PTCDA (3,4,9,10-perylene-tetracarboxylicacid-dianhydride) molecule from a Ag(111) substrate using the dynamic mode method with an Ag-covered tungsten AFM tip. The authors were able to follow the lifting process (Fig. 2c,d) monitoring the junction stiffness as the molecule was peeled off the surface to yield a vertically bound molecule, which could also be characterized electronically to determine the conductance through the vertical metal–molecule–metal junction with an idealized geometry. These measurements were supported by force field-based model calculations (Fig. 2c and dashed black line in Fig. 2d), presenting a way to correlate local geometry to the electronic transport.Extending the work from metal point contacts, ambient meas-urements of force and conductance across single-molecule junc-tions have been carried out using the static AFM mode 33. These measurements allow correlation of the bond rupture forces with the chemistry of the linker group and molecular backbone. Single-molecule junctions are formed between a Au-metal sub-strate and a Au-coated cantilever in an environment of molecules. Measurements of current through the junction under an applied bias determine conductance, while simultaneous measurements of cantilever deflection relate to the force applied across the junction as shown in Fig. 2e. Although measurements of current throughzF zyxCantileverIVabConductance G (G 0)1 2 3Tip–sample distance d (Å)S h o r t -r a n g e f o r c e F z (n N )10−310−210−11110−110−210−3e10−410−210C o n d u c t a n c e (G 0)Displacement86420Force (nN)0.5 nm420−2F o r c e (n N )−0.4−0.200.20.4Displacement (nm)SSfIncreasing rupture forcegc(iv)(i)(iii)(ii)Low HighCounts d9630−3d F /d z (n N n m −1)(i)(iv)(iii)(ii)A p p r o a chL i ft i n g110−210−4G (2e 2/h )2051510z (Å)H 2NNH 2H 2NNH 2NNFigure 2 | Simultaneous measurements of electronic transport and mechanics. a , A conducting AFM set-up with a stiff probe (shown schematically) enabled the atomic-resolution imaging of a Pt adsorbate on a Pt(111) surface (tan colour topography), before the simultaneous measurement of interatomic forces and currents. F z , short-range force. b , Semilogarithmic plot of tunnelling conductance and F z measured over the Pt atom. A similar decay constant for current and force as a function of interatomic distance is seen. The blue dashed lines are exponential fits to the data. c , Structural snapshots showing a molecular mechanics simulation of a PTCDA molecule held between a Ag substrate and tip (read right to left). It shows the evolution of the Ag–PTCDA–Ag molecular junction as a function of tip–surface distance. d , Upper panel shows experimental stiffness (d F /d z ) measurements during the lifting process performed with a conducting AFM. The calculated values from the simulation are overlaid (dashed black line). Lower panel shows simultaneously measured conductance (G ). e , Simultaneously measured conductance (red) and force (blue) measurements showing evolution of a molecular junction as a function of junction elongation. A Au point contact is first formed, followed by the formation of a single-molecule junction, which then ruptures on further elongation. f , A two-dimensional histogram of thousands of single-molecule junctionrupture events (for 1,4-bis(methyl sulphide) butane; inset), constructed by redefining the rupture location as the zero displacement point. The most frequently measured rupture force is the drop in force (shown by the double-headed arrow) at the rupture location in the statistically averaged force trace (overlaid black curve). g , Beyond the expected dependence on the terminal group, the rupture force is also sensitive to the molecular backbone, highlighting the interplay between chemical structure and mechanics. In the case of nitrogen-terminated molecules, rupture force increases fromaromatic amines to aliphatic amines and the highest rupture force is for molecules with pyridyl moieties. Figure reproduced with permission from: a ,b , ref. 36, © 2011 APS; c ,d , ref. 43, © 2011 APS.DOI: 10.1038/NNANO.2013.91such junctions are easily accomplished using standard instru-mentation, measurements of forces with high resolution are not straightforward. This is because a rather stiff cantilever (with a typical spring constant of ~50 N m−1) is typically required to break the Au point contact that is first formed between the tip and sub-strate, before the molecular junctions are created. The force reso-lution is then limited by the smallest deflection of the cantilever that can be measured. With a custom-designed system24 our group has achieved a cantilever displacement resolution of ~2 pm (com-pare with Au atomic diameter of ~280 pm) using an optical detec-tion scheme, allowing the force noise floor of the AFM set-up to be as low as 0.1 nN even with these stiff cantilevers (Fig. 2e). With this system, and a novel analysis technique using two-dimensional force–displacement histograms as illustrated in Fig. 2f, we have been able to systematically probe the influence of the chemical linker group44,45 and the molecular backbone46 on single-molecule junction rupture force as illustrated in Fig. 2g.Significant future opportunities with force measurements exist for investigations that go beyond characterizations of the junc-tion rupture force. In two independent reports, one by our group47 and another by Wagner et al.48, force measurements were used to quantitatively measure the contribution of van der Waals interac-tions at the single-molecule level. Wagner et al. used the stiffness data from the lifting of PTCDA molecules on a Au(111) surface, and fitted it to the stiffness calculated from model potentials to estimate the contribution of the various interactions between the molecule and the surface48. By measuring force and conductance across single 4,4’-bipyridine molecules attached to Au electrodes, we were able to directly quantify the contribution of van der Waals interactions to single-molecule-junction stiffness and rupture force47. These experimental measurements can help benchmark the several theoretical frameworks currently under development aiming to reliably capture van der Waals interactions at metal/ organic interfaces due to their importance in diverse areas includ-ing catalysis, electronic devices and self-assembly.In most of the experiments mentioned thus far, the measured forces were typically used as a secondary probe of junction prop-erties, instead relying on the junction conductance as the primary signature for the formation of the junction. However, as is the case in large biological molecules49, forces measured across single-mol-ecule junctions can also provide the primary signature, thereby making it possible to characterize non-conducting molecules that nonetheless do form junctions. Furthermore, molecules pos-sess many internal degrees of motion (including vibrations and rotations) that can directly influence the electronic transport50, and the measurement of forces with such molecules can open up new avenues for mechanochemistry51. This potential of using force measurements to elucidate the fundamentals of electronic transport and binding interactions at the single-molecule level is prompting new activity in this area of research52–54. Optoelectronics and optical spectroscopyAddressing optical properties and understanding their influence on electronic transport in individual molecular-scale devices, col-lectively referred to as ‘molecular optoelectronics’, is an area with potentially important applications55. However, the fundamental mismatch between the optical (typically, approximately at the micrometre scale) and molecular-length scales has historically presented a barrier to experimental investigations. The motiva-tions for single-molecule optoelectronic studies are twofold: first, optical spectroscopies (especially Raman spectroscopy) could lead to a significantly better characterization of the local junction structure. The nanostructured metallic electrodes used to real-ize single-molecule junctions are coincidentally some of the best candidates for local field enhancement due to plasmons (coupled excitations of surface electrons and incident photons). This there-fore provides an excellent opportunity for understanding the interaction of plasmons with molecules at the nanoscale. Second, controlling the electronic transport properties using light as an external stimulus has long been sought as an attractive alternative to a molecular-scale field-effect transistor.Two independent groups have recently demonstrated simulta-neous optical and electrical measurements on molecular junctions with the aim of providing structural information using an optical probe. First, Ward et al.56 used Au nanogaps formed by electromi-gration57 to create molecular junctions with a few molecules. They then irradiated these junctions with a laser operating at a wavelength that is close to the plasmon resonance of these Au nanogaps to observe a Raman signal attributable to the molecules58 (Fig. 3a). As shown in Fig. 3b, they observed correlations between the intensity of the Raman features and magnitude of the junction conductance, providing direct evidence that Raman signatures could be used to identify junction structures. They later extended this experimental approach to estimate vibrational and electronic heating in molecu-lar junctions59. For this work, they measured the ratio of the Raman Stokes and anti-Stokes intensities, which were then related to the junction temperature as a function of the applied bias voltage. They found that the anti-Stokes intensity changed with bias voltage while the Stokes intensity remained constant, indicating that the effective temperature of the Raman-active mode was affected by passing cur-rent through the junction60. Interestingly, Ward et al. found that the vibrational mode temperatures exceeded several hundred kelvin, whereas earlier work by Tao and co-workers, who used models for junction rupture derived from biomolecule research, had indicated a much smaller value (~10 K) for electronic heating61. Whether this high temperature determined from the ratio of the anti-Stokes to Stokes intensities indicates that the electronic temperature is also similarly elevated is still being debated55, however, one can definitely conclude that such measurements under a high bias (few hundred millivolts) are clearly in a non-equilibrium transport regime, and much more research needs to be performed to understand the details of electronic heating.Concurrently, Liu et al.62 used the STM-based break-junction technique8 and combined this with Raman spectroscopy to per-form simultaneous conductance and Raman measurements on single-molecule junctions formed between a Au STM tip and a Au(111) substrate. They coupled a laser to a molecular junction as shown in Fig. 3c with a 4,4’-bipyridine molecule bridging the STM tip (top) and the substrate (bottom). Pyridines show clear surface-enhanced Raman signatures on metal58, and 4,4’-bipy-ridine is known to form single-molecule junctions in the STM break-junction set-up8,15. Similar to the study of Ward et al.56, Liu et al.62 found that conducting molecular junctions had a Raman signature that was distinct from the broken molecu-lar junctions. Furthermore, the authors studied the spectra of 4,4’-bipyridine at different bias voltages, ranging from 10 to 800 mV, and reported a reversible splitting of the 1,609 cm–1 peak (Fig. 3d). Because this Raman signature is due to a ring-stretching mode, they interpreted this splitting as arising from the break-ing of the degeneracy between the rings connected to the source and drain electrodes at high biases (Fig. 3c). Innovative experi-ments such as these have demonstrated that there is new physics to be learned through optical probing of molecular junctions, and are initiating further interest in understanding the effect of local structure and vibrational effects on electronic transport63. Experiments that probe electroluminescence — photon emis-sion induced by a tunnelling current — in these types of molec-ular junction can also offer insight into structure–conductance correlations. Ho and co-workers have demonstrated simultaneous measurement of differential conductance and photon emissionDOI: 10.1038/NNANO.2013.91from individual molecules at a submolecular-length scale using an STM 64,65. Instead of depositing molecules directly on a metal sur-face, they used an insulating layer to decouple the molecule from the metal 64,65 (Fig. 3e). This critical factor, combined with the vac-uum gap with the STM tip, ensures that the metal electrodes do not quench the radiated photons, and therefore the emitted photons carry molecular fingerprints. Indeed, the experimental observation of molecular electroluminescence of C 60 monolayers on Au(110) by Berndt et al.66 was later attributed to plasmon-mediated emission of the metallic electrodes, indirectly modulated by the molecule 67. The challenge of finding the correct insulator–molecule combination and performing the experiments at low temperature makes electro-luminescence relatively uncommon compared with the numerous Raman studies; however, progress is being made on both theoretical and experimental fronts to understand and exploit emission pro-cesses in single-molecule junctions 68.Beyond measurements of the Raman spectra of molecular junctions, light could be used to control transport in junctions formed with photochromic molecular backbones that occur in two (or more) stable and optically accessible states. Some common examples include azobenzene derivatives, which occur in a cis or trans form, as well as diarylene compounds that can be switched between a conducting conjugated form and a non-conducting cross-conjugated form 69. Experiments probing the conductance changes in molecular devices formed with such compounds have been reviewed in depth elsewhere 70,71. However, in the single-mol-ecule context, there are relatively few examples of optical modula-tion of conductance. To a large extent, this is due to the fact that although many molecular systems are known to switch reliably in solution, contact to metallic electrodes can dramatically alter switching properties, presenting a significant challenge to experi-ments at the single-molecule level.Two recent experiments have attempted to overcome this chal-lenge and have probed conductance changes in single-molecule junctions while simultaneously illuminating the junctions with visible light 72,73. Battacharyya et al.72 used a porphyrin-C 60 ‘dyad’ molecule deposited on an indium tin oxide (I TO) substrate to demonstrate the light-induced creation of an excited-state mol-ecule with a different conductance. The unconventional transpar-ent ITO electrode was chosen to provide optical access while also acting as a conducting electrode. The porphyrin segment of the molecule was the chromophore, whereas the C 60 segment served as the electron acceptor. The authors found, surprisingly, that the charge-separated molecule had a much longer lifetime on ITO than in solution. I n the break-junction experiments, the illuminated junctions showed a conductance feature that was absent without1 μm Raman shift (cm –1)1,609 cm –1(–)Source 1,609 cm–1Drain (+)Low voltage High voltageMgPNiAl(110)STM tip (Ag)VacuumThin alumina 1.4 1.5 1.6 1.701020 3040200400Photon energy (eV)3.00 V 2.90 V 2.80 V 2.70 V 2.60 V2.55 V 2.50 VP h o t o n c o u n t s (a .u .)888 829 777731Wavelength (nm)Oxideacebd f Raman intensity (CCD counts)1,5001,00050000.40.30.20.10.01,590 cm −11,498 cm −1d I /d V (μA V –1)1,609 cm –11,631 cm–11 μm1 μmTime (s)Figure 3 | Simultaneous studies of optical effects and transport. a , A scanning electron micrograph (left) of an electromigrated Au junction (light contrast) lithographically defined on a Si substrate (darker contrast). The nanoscale gap results in a ‘hot spot’ where Raman signals are enhanced, as seen in the optical image (right). b , Simultaneously measured differential conductance (black, bottom) and amplitudes of two molecular Raman features (blue traces, middle and top) as a function of time in a p-mercaptoaniline junction. c , Schematic representation of a bipyridine junction formed between a Au STM tip and a Au(111) substrate, where the tip enhancement from the atomically sharp STM tip results in a large enhancement of the Raman signal. d , The measured Raman spectra as a function of applied bias indicate breaking of symmetry in the bound molecule. e , Schematic representation of a Mg-porphyrin (MgP) molecule sandwiched between a Ag STM tip and a NiAl(110) substrate. A subnanometre alumina insulating layer is a key factor in measuring the molecular electroluminescence, which would otherwise be overshadowed by the metallic substrate. f , Emission spectra of a single Mg-porphyrin molecule as a function of bias voltage (data is vertically offset for clarity). At high biases, individual vibronic peaks become apparent. The spectra from a bare oxide layer (grey) is shown for reference. Figure reproduced with permission from: a ,b , ref. 56, © 2008 ACS; c ,d , ref. 62, © 2011 NPG; e ,f , ref. 65, © 2008 APS.DOI: 10.1038/NNANO.2013.91light, which the authors assigned to the charge-separated state. In another approach, Lara-Avila et al.73 have reported investigations of a dihydroazulene (DHA)/vinylheptafulvene (VHF) molecule switch, utilizing nanofabricated gaps to perform measurements of Au–DHA–Au single-molecule junctions. Based on the early work by Daub et al.74, DHA was known to switch to VHF under illumina-tion by 353-nm light and switch back to DHA thermally. In three of four devices, the authors observed a conductance increase after irradiating for a period of 10–20 min. In one of those three devices, they also reported reversible switching after a few hours. Although much more detailed studies are needed to establish the reliability of optical single-molecule switches, these experiments provide new platforms to perform in situ investigations of single-molecule con-ductance under illumination.We conclude this section by briefly pointing to the rapid pro-gress occurring in the development of optical probes at the single-molecule scale, which is also motivated by the tremendous interest in plasmonics and nano-optics. As mentioned previously, light can be coupled into nanoscale gaps, overcoming experimental chal-lenges such as local heating. Banerjee et al.75 have exploited these concepts to demonstrate plasmon-induced electrical conduction in a network of Au nanoparticles that form metal–molecule–metal junctions between them (Fig. 3f). Although not a single-molecule measurement, the control of molecular conductance through plas-monic coupling can benefit tremendously from the diverse set of new concepts under development in this area, such as nanofabri-cated transmission lines 76, adiabatic focusing of surface plasmons, electrical excitation of surface plasmons and nanoparticle optical antennas. The convergence of plasmonics and electronics at the fundamental atomic- and molecular-length scales can be expected to provide significant opportunities for new studies of light–mat-ter interaction 77–79.Thermoelectric characterization of single-molecule junctions Understanding the electronic response to heating in a single-mole-cule junction is not only of basic scientific interest; it can have a tech-nological impact by improving our ability to convert wasted heat into usable electricity through the thermoelectric effect, where a temper-ature difference between two sides of a device induces a voltage drop across it. The efficiency of such a device depends on its thermopower (S ; also known as the Seebeck coefficient), its electric and thermal conductivity 80. Strategies for increasing the efficiency of thermoelec-tric devices turned to nanoscale devices a decade ago 81, where one could, in principle, increase the electronic conductivity and ther-mopower while independently minimizing the thermal conductiv-ity 82. This has motivated the need for a fundamental understandingof thermoelectrics at the single-molecule level 83, and in particular, the measurement of the Seebeck coefficient in such junctions. The Seebeck coefficient, S = −(ΔV /ΔT )|I = 0, determines the magnitude of the voltage developed across the junction when a temperature dif-ference ΔT is applied, as illustrated in Fig. 4a; this definition holds both for bulk devices and for single-molecule junctions. If an addi-tional external voltage ΔV exists across the junction, then the cur-rent I through the junction is given by I = G ΔV + GS ΔT where G is the junction conductance 83. Transport through molecular junctions is typically in the coherent regime where conductance, which is pro-portional to the electronic transmission probability, is given by the Landauer formula 84. The Seebeck coefficient at zero applied voltage is then related to the derivative of the transmission probability at the metal Fermi energy (in the off-resonance limit), with, S = −∂E ∂ln( (E ))π2k 2B T E 3ewhere k B is the Boltzmann constant, e is the charge of the electron, T (E ) is the energy-dependent transmission function and E F is the Fermi energy. When the transmission function for the junction takes on a simple Lorentzian form 85, and transport is in the off-resonance limit, the sign of S can be used to deduce the nature of charge carriers in molecular junctions. In such cases, a positive S results from hole transport through the highest occupied molecu-lar orbital (HOMO) whereas a negative S indicates electron trans-port through the lowest unoccupied molecular orbital (LUMO). Much work has been performed on investigating the low-bias con-ductance of molecular junctions using a variety of chemical linker groups 86–89, which, in principle, can change the nature of charge carriers through the junction. Molecular junction thermopower measurements can thus be used to determine the nature of charge carriers, correlating the backbone and linker chemistry with elec-tronic aspects of conduction.Experimental measurements of S and conductance were first reported by Ludoph and Ruitenbeek 90 in Au point contacts at liquid helium temperatures. This work provided a method to carry out thermoelectric measurements on molecular junctions. Reddy et al.91 implemented a similar technique in the STM geome-try to measure S of molecular junctions, although due to electronic limitations, they could not simultaneously measure conductance. They used thiol-terminated oligophenyls with 1-3-benzene units and found a positive S that increased with increasing molecular length (Fig. 4b). These pioneering experiments allowed the iden-tification of hole transport through thiol-terminated molecular junctions, while also introducing a method to quantify S from statistically significant datasets. Following this work, our group measured the thermoelectric current through a molecular junction held under zero external bias voltage to determine S and the con-ductance through the same junction at a finite bias to determine G (ref. 92). Our measurements showed that amine-terminated mol-ecules conduct through the HOMO whereas pyridine-terminatedmolecules conduct through the LUMO (Fig. 4b) in good agree-ment with calculations.S has now been measured on a variety of molecular junctionsdemonstrating both hole and electron transport 91–95. Although the magnitude of S measured for molecular junctions is small, the fact that it can be tuned by changing the molecule makes these experiments interesting from a scientific perspective. Future work on the measurements of the thermal conductance at the molecu-lar level can be expected to establish a relation between chemical structure and the figure of merit, which defines the thermoelec-tric efficiencies of such devices and determines their viability for practical applications.SpintronicsWhereas most of the explorations of metal–molecule–metal junc-tions have been motivated by the quest for the ultimate minia-turization of electronic components, the quantum-mechanical aspects that are inherent to single-molecule junctions are inspir-ing entirely new device concepts with no classical analogues. In this section, we review recent experiments that demonstrate the capability of controlling spin (both electronic and nuclear) in single-molecule devices 96. The early experiments by the groups of McEuen and Ralph 97, and Park 98 in 2002 explored spin-depend-ent transport and the Kondo effect in single-molecule devices, and this topic has recently been reviewed in detail by Scott and Natelson 99. Here, we focus on new types of experiment that are attempting to control the spin state of a molecule or of the elec-trons flowing through the molecular junction. These studies aremotivated by the appeal of miniaturization and coherent trans-port afforded by molecular electronics, combined with the great potential of spintronics to create devices for data storage and quan-tum computation 100. The experimental platforms for conducting DOI: 10.1038/NNANO.2013.91。
219410556_盐酸胺碘酮注射液与葡萄糖注射液配伍在不同材质给药器具中的稳定性
盐酸胺碘酮注射液与葡萄糖注射液配伍在不同材质给药器具中的稳定性薛晓1陈辰1曾垂宇2(1. 上海上药第一生化药业有限公司上海 200240,2. 上海上药创新医药技术有限公司上海 200020)摘要目的:考察盐酸胺碘酮注射液在不含酞酸二乙酯(diethyl phthatate, DEP)的不同材质给药器具中和葡萄糖注射液的配伍稳定性。
方法:根据盐酸胺碘酮注射液用法用量的要求,用葡萄糖注射液配制不同浓度的胺碘酮注射液,测定其在不含DEP的不同材质输液器、中心静脉导管、注射器中的稳定性。
结果:盐酸胺碘酮注射液中苯甲醇在内层涂料热塑性聚氨酯材质的输液器中出现微量的吸附,其他各考察指标无明显变化。
结论:盐酸胺碘酮注射液在不含DEP 的不同材质给药器具中与葡萄糖注射液配伍稳定。
关键词盐酸胺碘酮输液器中心静脉导管葡萄糖注射液配伍中图分类号:R972.2 文献标志码:A 文章编号:1006-1533(2023)11-0075-05引用本文薛晓, 陈辰, 曾垂宇. 盐酸胺碘酮注射液与葡萄糖注射液配伍在不同材质给药器具中的稳定性[J]. 上海医药, 2023, 44(11): 75-79.Stability of amiodarone hydrochloride injection and glucose injectionin different drug delivery devicesXUE Xiao1, CHEN Chen1, ZENG Chuiyu2(1. SPH No.1 Biochemical & Pharmaceutical Co., Ltd., Shanghai 200240, China; 2. Shanghai SPHInnovative Pharmaceutical Technology Co., Ltd., Shanghai 200020, China)ABSTRACT Objective: To investigate the compatibility stability of amiodarone hydrochloride injection with glucose injection in drug delivery devices consisting of different materials without diethyl phthatate (DEP). Methods: Different concentrations of amiodarone were prepared with glucose injection according to the requirements of its usage and dosage and its stability in infusion sets, central venous catheters and syringes without DEP was determined. Results: The benzyl alcohol in the amiodarone hydrochloride injection was slightly adsorbed in the infusion set with the inner coating made of thermoplastic polyurethanes (TPU), while there was no significant change in other indicators. Conclusion: The compatibility of amiodarone hydrochloride injection in DEHP-free drug delivery devices made of different materials with glucose injection is stable.KEY WORDS amiodarone hydrochloride; infusion set; central venous catheter; glucose injection; compatibility盐酸胺碘酮注射液属于一种抗心律失常的药物,目前被广泛应用于临床治疗严重的心律失常,其药理作用非常广泛而独特,能使快速心房颤动转复为窦性心律。
医药行业专业英语词汇
医药行业专业英语词汇(非常有用)FDA和EDQM术语: CLINICAL?TRIAL:临床试验? ANIMAL?TRIAL:动物试验? ACCELERATED?APPROVAL:加速批准? STANDARD?DRUG:标准药物? INVESTIGATOR:研究人员;调研人员PREPARING?AND?SUBMITTING:起草和申报? SUBMISSION:申报;递交? BENIFIT(S):受益? RISK (S):受害? DRUG?PRODUCT:药物产品? DRUG?SUBSTANCE:原料药? ESTABLISHED?NAME:确定的名称? GENERIC?NAME:非专利名称? PROPRIETARY?NAME:专有名称;? INN (INTERNATIONAL?NONPROPRIETARY?NAME):国际非专有名称? ADVERSE?EFFECT:副作用? ADVERSE?REACTION:不良反应? PROTOCOL:方案? ARCHIVAL?COPY:存档用副本? REVIEW?COPY:审查用副本? OFFICIAL?COMPENDIUM:法定药典(主要指USP、?NF).? USP(THE?UNITED?STATES?PHARMACOPEIA):美国药典 NF(NATIONAL?FORMULARY):(美国)国家处方集? OFFICIAL=PHARMACOPEIAL=?COMPENDIAL:药典的;法定的;官方的? AGENCY:审理部门(指FDA)? IDENTITY:真伪;鉴别;特性? STRENGTH:规格;规格含量(每一剂量单位所含有效成分的量)? LABELED?AMOUNT:标示量? REGULATORY?SPECIFICATION:质量管理规格标准(NDA提供)? REGULATORY?METHODOLOGY:质量管理方法? REGULATORY?METHODS?VALIDATION:管理用分析方法的验证 COS/CEP?欧洲药典符合性认证 ICH(International?Conference?on?Harmonization?of?Technical?Requirements?for?Registrat ion?of?Pharmaceuticals?for?Human?Use)人用药物注册技术要求国际协调会议 ICH文件分为质量、安全性、有效性和综合学科4类。
温敏水凝胶的英语
温敏水凝胶的英语The English Composition on Thermo-Sensitive HydrogelsThermo-sensitive hydrogels have gained significant attention in the field of biomedicine due to their unique properties and potential applications. These intelligent materials possess the ability to undergo reversible phase transitions in response to changes in temperature, making them particularly useful in various biomedical applications.Hydrogels are a class of hydrophilic polymeric networks that can absorb and retain large amounts of water or biological fluids within their three-dimensional structure. Thermo-sensitive hydrogels, specifically, exhibit a temperature-dependent phase transition, which means they can undergo a sol-gel transition as the temperature changes. This property is often referred to as the lower critical solution temperature (LCST) or upper critical solution temperature (UCST), depending on the specific polymer system.One of the most well-known thermo-sensitive hydrogels is poly(N-isopropylacrylamide) (PNIPAAm), wh ich has an LCST around 32°C, close to the human body temperature. Below the LCST, PNIPAAmhydrogels are in a swollen, hydrophilic state, allowing for the incorporation and release of various therapeutic agents. However, as the temperature increases above the LCST, the polymer chains undergo a conformational change, leading to the collapse of the hydrogel structure and the expulsion of water. This temperature-induced phase transition makes PNIPAAm-based hydrogels particularly useful for controlled drug delivery applications.The mechanism behind the temperature-responsive behavior of thermo-sensitive hydrogels, such as PNIPAAm, is related to the delicate balance between hydrophobic and hydrophilic interactions within the polymer network. At temperatures below the LCST, the polymer chains are hydrated, and the hydrogen bonding between water molecules and the polymer's amide groups dominates, leading to a swollen, hydrophilic state. As the temperature increases above the LCST, the hydrogen bonding between water and the polymer becomes weaker, and the hydrophobic interactions between the isopropyl groups of the polymer become more prominent. This results in the collapse of the polymer chains, causing the expulsion of water and the formation of a more compact, hydrophobic structure.The unique temperature-responsive behavior of thermo-sensitive hydrogels has led to their widespread application in various biomedical fields. One of the primary applications is in controlleddrug delivery systems. Thermo-sensitive hydrogels can be used as carriers for therapeutic agents, such as small-molecule drugs, proteins, or even cells. These hydrogels can be designed to release the encapsulated drugs in a controlled manner by responding to the temperature changes in the body. For example, a PNIPAAm-based hydrogel loaded with a drug can be administered in a liquid state at room temperature and then undergo a phase transition to a gel state upon reaching body temperature, effectively trapping the drug within the hydrogel matrix. As the temperature increases further, the hydrogel can undergo a volume phase transition, leading to the release of the drug in a controlled manner.Another important application of thermo-sensitive hydrogels is in tissue engineering and regenerative medicine. These hydrogels can be used as scaffolds for cell growth and tissue regeneration. The temperature-responsive nature of the hydrogels allows for easy administration and in situ gelation, which can facilitate the encapsulation of cells or the delivery of growth factors directly to the site of injury or disease. The hydrogel scaffold can then provide a suitable microenvironment for cell proliferation, differentiation, and tissue formation.Thermo-sensitive hydrogels have also found applications in wound healing and burn treatment. The ability of these hydrogels to undergo a sol-gel transition in response to temperature changes canbe exploited to create wound dressings that can be easily applied in a liquid form and then transition to a gel state upon contact with the body. This can help maintain a moist environment, promote wound healing, and prevent infection.Furthermore, thermo-sensitive hydrogels have been investigated for use in various diagnostic and sensing applications. For instance, they can be designed to incorporate responsive elements, such as enzyme-substrate pairs or antibody-antigen interactions, which can trigger a detectable change in the hydrogel's physical properties in response to the presence of specific analytes or biomarkers.The development of thermo-sensitive hydrogels has also led to advancements in the field of injectable biomaterials. These hydrogels can be designed to be injected in a liquid form and then undergo in situ gelation at the target site, allowing for minimally invasive procedures and the delivery of therapeutic agents or cells directly to the site of interest.Despite the numerous promising applications of thermo-sensitive hydrogels, there are still several challenges that need to be addressed. One of the key challenges is the optimization of the LCST or UCST to match the specific requirements of the target application. Researchers are exploring ways to fine-tune the polymer composition and structure to achieve the desired temperature-responsive behavior. Additionally, the long-term biocompatibility and biodegradability of these hydrogels need to be thoroughly investigated to ensure their safe and effective use in biomedical applications.In conclusion, thermo-sensitive hydrogels have emerged as a versatile class of biomaterials with tremendous potential in the field of biomedical engineering. Their temperature-responsive behavior, coupled with their ability to encapsulate and deliver therapeutic agents, make them a promising platform for a wide range of applications, from controlled drug delivery to tissue engineering and regenerative medicine. As research in this field continues to advance, we can expect to see even more innovative and impactful applications of thermo-sensitive hydrogels in the years to come.。
开启片剂完整性的窗户(中英文对照)
开启片剂完整性的窗户日本东芝公司,剑桥大学摘要:由日本东芝公司和剑桥大学合作成立的公司向《医药技术》解释了FDA支持的技术如何在不损坏片剂的情况下测定其完整性。
太赫脉冲成像的一个应用是检查肠溶制剂的完整性,以确保它们在到达肠溶之前不会溶解。
关键词:片剂完整性,太赫脉冲成像。
能够检测片剂的结构完整性和化学成分而无需将它们打碎的一种技术,已经通过了概念验证阶段,正在进行法规申请。
由英国私募Teraview公司研发并且以太赫光(介于无线电波和光波之间)为基础。
该成像技术为配方研发和质量控制中的湿溶出试验提供了一个更好的选择。
该技术还可以缩短新产品的研发时间,并且根据厂商的情况,随时间推移甚至可能发展成为一个用于制药生产线的实时片剂检测系统。
TPI技术通过发射太赫射线绘制出片剂和涂层厚度的三维差异图谱,在有结构或化学变化时太赫射线被反射回。
反射脉冲的时间延迟累加成该片剂的三维图像。
该系统使用太赫发射极,采用一个机器臂捡起片剂并且使其通过太赫光束,用一个扫描仪收集反射光并且建成三维图像(见图)。
技术研发太赫技术发源于二十世纪九十年代中期13本东芝公司位于英国的东芝欧洲研究中心,该中心与剑桥大学的物理学系有着密切的联系。
日本东芝公司当时正在研究新一代的半导体,研究的副产品是发现了这些半导体实际上是太赫光非常好的发射源和检测器。
二十世纪九十年代后期,日本东芝公司授权研究小组寻求该技术可能的应用,包括成像和化学传感光谱学,并与葛兰素史克和辉瑞以及其它公司建立了关系,以探讨其在制药业的应用。
虽然早期的结果表明该技术有前景,但日本东芝公司却不愿深入研究下去,原因是此应用与日本东芝公司在消费电子行业的任何业务兴趣都没有交叉。
这一决定的结果是研究中心的首席执行官DonArnone和剑桥桥大学物理学系的教授Michael Pepper先生于2001年成立了Teraview公司一作为研究中心的子公司。
TPI imaga 2000是第一个商品化太赫成像系统,该系统经优化用于成品片剂及其核心完整性和性能的无破坏检测。
同位素稀释质谱法测定血清肌酐浓度的不确定度评定
作者简介:张卫威,男,1989年生,博士,主要从事生物化学与分子生物学研究。
通信作者:王惠民,E-mail :。
同位素稀释质谱法测定血清肌酐浓度的不确定度评定张卫威, 景蓉蓉, 季伙燕, 王建新, 王 峰, 王惠民(南通大学附属医院检验科,江苏 南通 226001)摘要:目的 探讨同位素稀释质谱法(ID-MS )测量血清肌酐浓度的不确定度评定方法。
方法 应用ID-MS 建立测定血清肌酐浓度的参考测量程序,严格按照《测量不确定度表示指南》(简称GUM )评定不确定度,即严格根据测量模型对各不确定度分量进行详细分析和定量,加减项用绝对值、乘除项用相对值合成各标准不确定度分量;同时,用传统评定方法和蒙特卡洛方法(MCM )分别对血清肌酐浓度测量结果进行不确定度评定。
结果 对特定血清进行赋值,其肌酐浓度为347.4 μmol/L 。
按照ID-MS 测量模型,并严格应用GUM 原理进行不确定度评定,其合成标准不确定度的相对值为0.89%,而传统方法评定结果为0.93%,较GUM 法高4.8%;采用MCM 评定各不确定度分量,其合成标准不确定度的相对值为0.64%,较严格GUM 法低27.9%,其测量结果的95%可信区间为343.1~351.8 μmol/L 。
结论 与GUM 法评定结果比较,传统评定方法结果偏高,而MCM 偏低,3种评定方法存在差异。
建议采用GUM 法,根据测量模型对各不确定度分量进行定量和合成。
有条件的话,可用MCM 评定不确定度。
关键词:同位素稀释质谱法;血清肌酐浓度;测量不确定度;蒙特卡洛方法Isotope dilution mass spectrometry applied in measurement uncertainty of serum creatinine concentration ZHANG Weiwei ,JING Rongrong ,JI Huoyan ,WANG Jianxin ,WANG Feng ,WANG Huimin.(Department of Clinical Laboratory ,Affiliated Hospital of Nantong University ,Nantong 226001,Jiangsu ,China )Abstract :Objective To investigate the evaluation method for measurement uncertainties of serum creatinine concentration by isotope dilution mass spectrometry (ID-MS ). Methods We established a reference measurement procedure for the serum creatinine concentration by ID-MS. The uncertainty was evaluated according to the strict Guide to the Expression of Measurement Uncertainty (GUM ) method. The strict GUM method meant that each uncertain component was analyzed and quantified in detail according to the measurement model. The calculation was based on the principle that relative values should be used in multiplication and division ,and absolute values should be used in addition and subtraction. Meanwhile ,traditional evaluation method and the Monte Carlo method (MCM ) were used to evaluate the uncertainties of serum creatinine concentration. Results The measured specific serum creatinine concentration was 347.4 μmol/L. The combined standard uncertainty was 0.89% according to ID-MS measurement model and strict GUM principle. The uncertainty evaluated by the traditional method was 0.93%,which was 4.8% higher than that evaluated by the GUM method. The combined standard uncertainty was 0.64% by MCM ,which was 27.9% lower than that evaluated by the strict GUM method. The 95% confidence interval of the measurement result by MCM was 343.1-351.8 μmol/L. Conclusions The result of uncertainty evaluated by the traditional method is higher than that of the strict GUM method ,while the result of the MCM is lower than that of the strict GUM method. It is suggested that each uncertainty component should be strictly quantified and combined according to the GUM method. If possible ,the MCM could be used to evaluate the uncertainty.Key words :Isotope dilution mass spectrometry ;Serum creatinine concentration ;Measurement uncertainty ;Monte Carlo method文章编号:1673-8640(2021)03-0263-07 中图分类号:R446 文献标志码:A DOI :10.3969/j.issn.1673-8640.2021.03.0071997年,国际计量委员会物质量咨询委员会在巴黎召开的第6次会议上,确认了同位素稀释质谱法(isotope dilution mass spectrometry ,ID-MS )、库仑法、重量法、滴定法和凝固点下降法是具有绝对测量性质的方法。
Optical Velocimetry Systems and Methods for Determ
专利名称:Optical Velocimetry Systems and Methodsfor Determining the Velocity of a Body UsingFringes Generated by a Spatial LightModulator发明人:Sandeep Tauro,Jean-Marc Muller申请号:US13600513申请日:20120831公开号:US20140063484A1公开日:20140306专利内容由知识产权出版社提供专利附图:摘要:A velocimetry system for measuring the velocity of a moving body propagatingthrough a measurement volume includes a light source for emitting a light beam, a controller for generating a modulation pattern corresponding to a desired set of fringes to be generated in the measurement volume, and a spatial light modulator operatively connected to the controller to receive therefrom the modulation pattern. The spatial light modulator is configured to spatially modulate the light beam according to the modulation pattern in order to generate the desired set of fringes in the measurement volume. Also provided are a light detector for measuring the energy of the light scattered by the moving body as it intersects the fringes, and a data analysis unit operatively connected to the light detector and adapted to determine the velocity of the moving body from at least one fringe characteristic and the energy of the scattered light measured.申请人:Sandeep Tauro,Jean-Marc Muller地址:Kobenhaven NV DK,Boston MA US国籍:DK,US更多信息请下载全文后查看。
微波裂解海藻快速制取生物燃油的试验
第26卷第1期农业工程学报Vol.26No.12010年1月Transactions of the CSAE Jan.2010295微波裂解海藻快速制取生物燃油的试验万益琴1,2,王应宽2,3,林向阳2,4,刘玉环1,2,陈灵2,李叶丛2,阮榕生1,2※(1.南昌大学生物质转化教育部工程研究中心,生命科学与食品工程学院,南昌330047;2.Center for Biorefining and Department of Bioproducts and Biosystems Engineering,University of Minnesota,St.Paul,MN55108,USA;3.农业部规划设计研究院,北京100125;4.福州大学生物科学与工程学院,福州350108)摘要:为了探索低成本的海藻生物油快速制取工艺技术,研究组已成功开发出一套海藻的选育、培养、收获、干制技术。
利用自行优选、培养、收获并干制的海藻粉,基于课题组在生物质的微波裂解技术已取得的突破,采用自行研制的玉米秸秆微波裂解的相关设备,对微波裂解海藻制取生物燃油的技术进行试验研究,获得大量在自然条件下可分层的海藻生物油。
采用气相色谱-质谱(GC-MS)分析了所得到的生物油产品中两相油组分,得到了生物油产品中的物质组成及其相对含量,可为海藻生物油的精制及其副产品的开发利用提供了参考。
研究表明,微波裂解海藻是一种低成本、快速、高效制取海藻生物燃油的方法,为海藻生物油的规模化生产提供参考。
关键词:海藻,微波,裂解,生物燃油,生物柴油,生物质能doi:10.3969/j.issn.1002-6819.2010.01.052中图分类号:TP751.1文献标识码:A文章编号:1002-6819(2010)-01-0295-06万益琴,王应宽,林向阳,等.微波裂解海藻快速制取生物燃油的试验[J].农业工程学报,2010,26(1):295-300.Wan Yiqin,Wang Yingkuan,Lin Xiangyang,et al.Experimental investigation on microwave assisted pyrolysis of algae for rapid bio-oil production[J].Transactions of the CSAE,2010,26(1):295-300.(in Chinese with English abstract)0引言第一代生物质能源技术是将玉米、甘蔗、高粱等农作物转化为生物乙醇[1],或是把大豆、油棕榈和油菜子等油类作物加工为生物柴油。
含油食品酸价检测不确定度评定
含油食品酸价检测不确定度评定张学英(湘西州食品药品检验所,湖南吉首416000)摘要酸价是反映食品中油脂酸败程度的重要指标,也是食用油或含油食品中必检项目之一。
酸价检测不确定度评定可以更科学地评价酸价检测的准确性、科学性和公平性。
本文采用冷溶剂指示剂滴定法与冷溶剂自动电位滴定法检测酸价,建立数学模型,分析不确定度来源,评定各不确定度分量、合成不确定度及扩展不确定度。
结果表明,自动电位滴定法检测某含油食品酸价结果为6.4mg/g,扩展不确定度为0.2mg/g,K=2,显著性不确定度分量为NaOH标准滴定液浓度;指示剂滴定法酸价为6.3mg/g,扩展不确定度为0.3mg/g,K=2,显著性不确定度为NaOH标准滴定液浓度不确定度最大、A类不确定度次之、滴定体积不确定度再次之,且三者显著性相近,扩展不确定度大于电位滴定法。
通过不确定度评定和显著性分析,可以有针对性地找到降低或控制检测误差的方法,提高检测结果准确性。
关键词含油食品;酸价;不确定度评定;冷溶剂指示剂滴定法;冷溶剂自动电位滴定法中图分类号TS207.3文献标识码A文章编号1007-5739(2024)04-0130-06DOI:10.3969/j.issn.1007-5739.2024.04.033开放科学(资源服务)标识码(OSID):Assessment of Uncertainty in Acid Value Detection of Oil-containing FoodZHANG Xueying(Xiangxi Prefecture Food and Drug Inspection Institute,Jishou Hunan416000) Abstract Acid value is an important index reflecting the rancidity degree of oil in food,it is also one of the items that must be tested in edible oil or oil-containing food.Assessment of uncertainty in acid value detection can scientifi-cally assess the accuracy,scientificity,and fairness of acid value detection.This paper used the cold solvent indicator titration and the cold solvent automatic potentiometric titration to detect acid value,established a mathematical model, analyzed the sources of uncertainty,then assessed each uncertainty component,combined uncertainty,and extended uncertainty.The results showed that,through the automatic potentiometric titration,the acid value detection of a certain oil-containing food indicated C=6.4mg/g,U=0.2mg/g,K=2,and the significant uncertainty component was the concen-tration of NaOH standard titration solution.The acid value detected by the indicator titration indicated C=6.3mg/g,U= 0.3mg/g,K=2.The concentration of NaOH standard titration solution had the highest significant uncertainty,followed by class A uncertainty,and the titration volume uncertainty,the three had the similar significance,and the extended uncertainty of indicator titration method was greater than that of the potentiometric method.Assessment of uncertainty and significance analysis,can find targeted methods to reduce or control detection errors and improve the accuracy of detection results.Keywords oil-containing food;acid value;uncertainty assessment;cold solvent indicator titration method;cold solvent automatic potentiometric titration method食用油脂及含油食品是人民日常生活必不可少的物质,油脂是中国居民膳食指南重要组成部分,是人体所需三大宏量营养素之一[1]。
马来酸氯苯那敏的非水测定
• 空白溶液消耗的高氯酸滴定液体积可以忽 略不计。
• 用二阶微商内插法确定滴定终点
V(ml) 4.50 4.60 4.70 4.80 4.90 5.00 5.10 5.20 5.30 5.40 5.50
E(mV) 393 398 403 411 422 436 461 531 558 570 578
手动电位滴定 平均含量
自动电位滴定 平均含量
W(g) 0.1032 0.1000 0.1029 0.1033 0.0954 0.1026
W(g) 0.1023 0.1019 0.1035 0.1003 0.1010 0.1060
V(mL) 5.151 4.977 5.129 5.150 4.762 5.122
[操作方法与实验步骤]
• 手动滴定法测定含量与精密度试验: 取马来酸氯苯那敏约0.1g,精密称 定,加冰醋酸10mL溶解于烧杯中, 加结晶紫指示液1滴,将盛有供试品 溶液的烧杯置电磁搅拌器上,浸入 电极(玻璃电极和饱和甘汞电极), 搅拌,并向滴定管中分次滴加滴定 液;开始时可每次加入较多的量, 搅拌,记录电位;至将近终点前, 则应每次加入少量,搅拌,记录电 位,至突跃点已过,仍应继续滴加 几次滴定液,并记录电位和终点溶 液颜色。将滴定的结果用空白试验 校正。每1ml的高氯酸滴定液 (0.1mol/L)相当于19.54mg的 C16H19ClN2·C4H4O4。
尼康全自动倒置显微镜图像工作站-TiE的配置及其视野矫正
尼康全自动倒置显微镜图像工作站-TiE的配置及其视野矫正张妍乐;袁粒星;马志贵;顾玲【摘要】尼康全自动倒置显微镜图像工作站-TiE(活细胞工作站)是用于高端生物科学研究领域的新型倒置显微镜系列,能提供高分辨率、灵活性高、可扩展等多种强大功能,为定时拍摄高清活细胞体外增殖、迁移等活动图像提供可能.将尼康全自动倒置显微镜图像工作站-TiE应用于细胞体外行为的实时观测,为细胞研究提供了一种新的、更高质量的检测手段.【期刊名称】《中国医疗器械信息》【年(卷),期】2018(024)004【总页数】2页(P76-77)【关键词】尼康全自动倒置显微镜图像工作站-TiE;活细胞工作站;配置;视野矫正【作者】张妍乐;袁粒星;马志贵;顾玲【作者单位】四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室四川成都 610000;四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室四川成都 610000;四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室四川成都 610000;四川大学华西第二医院出生缺陷与相关妇儿疾病教育部重点实验室四川成都 610000【正文语种】中文【中图分类】Q2-33活细胞工作站(Live cell Imaging System,LIS)又称活细胞显微成像采集系统,是指在模拟体内环境的条件下,体外观测活细胞增殖、迁移、粘附、凋亡等多种活动的监测系统[1]。
能自分子水平上,做到基因定位定量表达动态分析、蛋白质合成降解运输动态研究、蛋白质相互作用动态研究以及代谢动力学测定等[2]。
因此,熟悉活细胞工作站的具体配置及其使用方法对临床工作具有重要意义。
本研究选取尼康全自动倒置显微镜图像工作站-TiE,介绍其配置及视野矫正方法,现具体报告如下。
1.配置1.1 显微镜目镜该显微镜包含CFI 10x目镜镜头,TI-TD双目镜筒D、TI-TS双目镜筒S、TI-TERG人机学镜筒等3种目镜筒。
【推荐下载】关于联苯双酯固体脂质纳米粒的体外释药特性评析
关于联苯双酯固体脂质纳米粒的体外释药特性评析【编者按】医药论文是科技论文的一种是用来进行医药科学研究和描述研究成果的论说性文章。
论文网为您提供医药论文范文参考,以及论文写作指导和格式排版要求,解决您在论文写作中的难题。
关于联苯双酯固体脂质纳米粒的体外释药特性评析【摘要】目的建立联苯双酯固体脂质纳米粒(DDB?SLN)体外释放的评价方法。
方法考察DDB?SLN 在不同介质中的平衡溶解度,并筛选出pH 7.4 磷酸盐缓冲液+40%(体积分数)乙醇作为合适的释药介质,采用动态透析释药法考察DDB?SLN 和药物溶液的体外释药情况。
结果DDB?SLN 在最初的0.5 h 内出现了突释现象,此后DDB?SLN 的释放符合一级动力学方程。
结论与药物溶液相比,DDB?SLN 具有明显的缓释效果。
【关键词】联苯双酯固体脂质纳米粒体外释药动态透析释药法联苯双酯(DDB)系合成五味子丙素的中间体,是20 世纪80 年代我国自行研制成功的抗肝炎药。
由于其降转氨酶作用显著,同时还能促进肝脏再生,具有保肝、护肝的功能,该药在我国及其他多个亚洲国家广泛应用于乙肝及丙肝的治疗[1]。
本文采用乳化蒸发-低温固化法[2]制备了联苯双酯固体脂质纳米粒(DDB?SLN),然后用动态透析法考察其体外释药情况,为体内研究提供了依据。
1 仪器及试剂1.1 仪器戴安P680 分析型高效液相色谱仪(美国);HJ?3 恒温磁力搅拌器(江苏金坛医疗器械厂);UV?2800 紫外可见分光光度计(尤尼柯仪器有限公司);AG245 型电子天平(德国Mettler Toledo 公司)。
1.2 试剂联苯双酯(浙江万邦药业有限公司,批号:070301);联苯双酯对照品(中国药品生物制品检定所,批号:0192?950150);DDB?SLN(自制);甲醇(色谱纯);透析袋(截留分子量:8 000~14 000);其他试剂均为分析纯。
2 实验方法2.1 DDB?SLN 的制备[3]称取DDB 5 mg、单硬脂酸甘油酯50 mg、卵磷脂80 mg 溶于适量的无水乙醇中,于(75 2)℃水浴下形成有机相;另取泊洛沙姆188 150 mg 溶于相同温度的水中,构成水相; 在搅拌(1 000 r/min)下将有机相用针头注入水相中,整个过程保持温度在脂质材料熔点以上。
Reed Instruments Model 8906 Thermo-Anemometer Inst
Thermo-AnemometerTable of ContentsFeatures (3)Specifications (3)Instrument Description (4)Operating Instructions ..............................................................5-10 Single Point Air Velocity (fpm) (5)Continuous Moving Average (5)MIN/MAX/AVG Reading on a Single Point (6)Multipoint Air Velocity Average (6)Auto Power OFF (7)Default unit setting (Imperial or Metric) (8)RS232 Output (9)Measuring fpm, MPH, Knot (km/hour) (9)Direct Single Point Air Flow Measure (cfm) (9)Multipoint Air Flow Average (cfm) (10)MIN/MAX/AVG for Single Point Air Volume (10)Battery Replacement (11)Troubleshooting (11)Features• Integral rotary vane sensor for one hand operation• Simultaneous display or air velocity and temperature• Continuous moving air velocity averaging of measurements for up to 2 hours• Displays Min/Max/Avg velocity with temperature values• Multi-point averaging up to 8 points• Calculate average velocity values in seconds• Selectable wind speed units: fpm, m/s, mph, km/h, knots• Calculate air volume by keying in area dimension• High-contrast, 4-digit LCD readout• RS-232 interface• Data hold and auto power offSpecificationsAir Velocity Range: 80 to 6900 fpm; 0.4 to 35 m/s; 0.9 to 78 mph;1.4 to 126 km/h; 0.8 to 68 knots Resolution: 1 fpm; 0.01 m/s, 0.1 mph; 0.1 km/h; 0.1 knot Accuracy: ±2% f.s.Temperature Range: -10 to 50°C (14 to 122°F)Resolution: 0.1 °C/°FAccuracy: ±0.6°C (±1.2°F)Power Supply: Single 9V batteryBattery Life: 400 hoursDimensions: 183(L) x 76(W) x 45(D)operating InstructionsFor the best results when using your instrument, make sure the airstream and the sensor are aligned as shown (±20 degrees maximum) and wait 3 seconds for the reading to stabilize.Single Point Air Velocity (fpm)1. Press the Power button to turn the meter on.The meter will show the full display for theinitial 5 seconds.2. The instrument is ready for use whenthe LCD display shows "vel" in the upperleft corner and temperature in the lowerright corner.Continuous Moving AverageThe meter has the ability to display continuous moving average for up to two (2) hours.1. Turn the power ON.2. Place the sensor in front of the airflow source.3.MIN/MAX/AVG Reading on a Single Point1. Turn the power ON.2. Place the sensor in front of the air flow source.3. Press the Max. / Min. button. The unit will begin to record thereadings. The meter displays the average velocity by default. Eachpress of the Max. / Min. button cycles the display through:• Real-time readings• MIN velocity• MAX velocity• AVG velocity4. To revert to normal measurement mode or clear the current MIN,MAX and AVG readings, turn OFF the meter, then turn it ON againor press and hold the Max. / Min. button until the meter beepstwice, then release.5. Note: Feet Per Minute (fpm) readings can be converted to CubicFeet per Minute (cfm) readings by following the instructions below:• Press the Hold button to store the readings before moving the meter away from an airflow source• Press the Mode button to enter area setting. After setting the area, press the Mode button again to convert fpm into cfm. Multipoint Air Velocity Average1. Turn the meter ON and position the vane atthe first point to be measured. As soon asthe first measurement is completed pressthe Hold button, (you will hear a singlebeep), and release. The display will show2.Press the Max. / Min. button, (you will hearshow a digit 1-8). This number represents the point number which has been recorded.3.points have been measured and recorded.one time.4.Once all the measurements have beenrecorded, press the Average button to view the average air velocity reading and the number of points which are recorded.5. Press the Hold button to revert to normal measurement mode.6.To clear multi-point average memory, press and hold the Average button until the unit beeps twice, then release.Auto Power OFFThe unit will turn off automatically after 20 minutes to save the battery. This will be preceded by 3 beeps. To disable auto power off:1. Turn the power OFF .2. Press the power button and the Holdbuttonat the same time and then release the Power button only. When an "n" appears on the LCD, you can release the Hold button. The instrument will remain on until the Power button is pressed.Default unit setting (Imperial or Metric)The default measuring units can be changed by following the steps below. The unit should be turned off before starting.1. Press and hold the Average button, thenpress the Power button once to turn theunit ON. When the LCD displays "ft/m","ms", "°C" and "°F", release the Averagebutton.2. To choose the metric units, press the Hold button. The LCDshould display "m/s" and "°C".To choose the imperial units, press the Average button. The LCDshould display "ft/m" and "°F".3. Press the Max. / Min. button. The LCD should display "S".Then press the Hold button. The LCD will display 2400 or 1200(pre-setting).RS232 Output1. Following Step 3 of Default unit setting, youwill see a "2400" (default) number on thescreen. The 2400 is the default setting ofBaud Rate for RS232 output. You canchange the setting to "1200" by pressingthe Hold button and to "2400" by pressingthe Average button.2. Please remember to save your changes by pressing theMax. / Min. button. An "S" will display on the LCD. Press theHold button to confirm and save the changed value. The meterwill return to air velocity mode automatically.3. Plug the earphone jack of the cable VZRS232M into the RS232socket on the meter and connect the 9-pin D-sub to the comput-er's COM1 or COM2. Press ON to start measurement. Measuring ft/m, MPH, Knot (km/hour)In imperial, press SEL:.MPH. KNOT, the reading will change from ft/m, mil/h, knot in turns.In metric, press SEL:.MPH. KNOT, the reading will change from m/s , km/h, knot in turns.Direct Single Point Air Flow Measure (cfm)Air Velocity measurement is calculated by multiplying the air velocity readings by the free area dimensions. You must first determine the free area of the air source before entering it into the meter.4. Press the Average button to advance to next number. Follow Step3 and repeat to input the free area size.5. Press the Mode button once all digits have been entered. Theword "FLOW" will appear. The meter is now ready to measure airflow (cfm).Multipoint Air Flow Average (cfm)1. Follow Step 1 to 4 of Multipoint Air Velocity Average.2. Press the Mode button once and confirm the correct free area set-ting is locked into the instrument. (If the free area setting must beadjusted, make the necessary changes now.)3. If the free area setting is correct, press the Mode button again toenter air flow mode.4. Unit will now display the average air flow reading and the numberof points measured.MIN/MAX/AVG for Single Point Air Volume1. Turn the power ON, select the mode as FLOW and the place thesensor in front of the air flow source.2. Press the Max. / Min. button, the unit will begin to record the read-ing. Press the Max. / Min. button to read the real time value, theMIN, the MAX and the AVG in turns. Long press the Max. / Min.button to clear the average readings.battery ReplacementIf the LCD display is flashing or there is no display, replacement of the battery is needed.1. Remove the screw from the lower back of the meter, open thebattery cover and remove the battery.2. Replace with a 9V battery and reinstall the cover.TroubleshootingError E6If the instruments' display shows E6, it indicates the related circuits or parts of the thermistor have failed. Send them back to the store where you bought the instrument for repair.Sensor's fan will not turnThis indicates the sensor fan is damaged, purchase a new sensor probe.notes _________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________ ________________________________________________。
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NASA/CR-2000-210118ICASE Report No. 2000-22Simultaneous Velocimetry and Thermometry of Air Using Nonresonant Heterodyned Laser-induced Thermal AcousticsRoger C. HartICASE, Hampton, VirginiaR. Jeffrey Balla and G.C. HerringNASA Langley Research Center, Hampton, VirginiaInstitute for Computer Applications in Science and EngineeringNASA Langley Research CenterHampton, VAOperated by Universities Space Research AssociationSIMULTANEOUS VELOCIMETRY AND THERMOMETRY OF AIR USINGNONRESONANT HETERODYNED LASER-INDUCED THERMAL ACOUSTICSROGER C. HART1, R. JEFFREY BALLA2, AND G. C. HERRING2Abstract. Non-resonant laser-induced thermal acoustics (LITA) is employed with heterodyne detection to measure temperature (285-295 K) and a single component of velocity (20-150 m/s) in an atmospheric pressure, subsonic, unseeded air jet. Good agreement is found with pitot-tube measurements of velocity (0.2% at 150 m/s and 2% at 20 m/s) and the isentropic expansion model for temperature (0.3%).Key words. velocimetry, thermometry, heterodyne, nonresonant Light Scattering, laser-induced thermal acoustics, LITASubject classification. Physical Sciences1. Introduction. Non-intrusive optical velocimetry is done either by intervalometry (e.g., particle image velocimetry or cross-correlation techniques) or by observing the Doppler shift ∆ω. Examples of the second approach are laser Doppler velocimetry (LDV) and planar Doppler velocimetry (PDV). Accurate measurement of the small Doppler shift found in typical subsonic aerodynamic applications is challenging. In PDV differential transmission at the absorption edge of an atomic line filter provides the necessary frequency resolution. With LDV, what is essentially a heterodyne technique is used. Both of these approaches require seeding with small particles. Seeding is inconvenient in some instances and impossible in others. Potential seeding problems include nonuniformities in the seed density, pitting of smooth model surfaces, seed particles lagging the flow, and the inability to seed specific locations (e.g., boundary layer).Laser-induced thermal acoustics (LITA) is a seedless diagnostic technique in which optical interference patterns induce sound waves and thermal gratings in the medium. Early gas-phase work [1-3] utilized a resonant version of LITA, in which the pump laser frequency was resonant with a molecular transition. Absorption in the molecule of interest, followed by thermalization, generated a stationary thermal grating and two moving acoustic gratings. These gratings were used to demonstrate sound speed measurements [4] versus pressure. More recently, nonresonant versions have been demonstrated for thermometry [5-7] and sound speed measurements[8] versus temperature. The nonresonant version generates only two moving acoustic gratings. Because nonresonant LITA does not require absorption, it is convenient for interrogation of a variety of atomic and molecular species. Hence, non-resonant LITA is attractive for application to a wide array of wind tunnels using various test gases. Most r ecently1 Institute for Computer Applications in Science and Engineering (ICASE), NASA Langley Research Center, Hampton, VA 23681-2199. This research was supported by the National Aeronautics and Space Administration under NASA Contract No. NAS1-97046 while the first author was in residence at the Institute for Computer Applications in Science and Engineering (ICASE), NASA Langley Research Center, Hampton, VA 23681-2199.2 AMDB, NASA Langley Research Center, Hampton, VA 23681-2199.heterodyned velocimetry[9] has been achieved with resonant LITA. In this letter, we demonstrate, simultaneously, heterodyned velocimetry and thermometry with nonresonant LITA at a single spatial point.2. Description of Heterodyne LITA Velocimetry. In nonresonant LITA, two crossed beams from a short-pulse pump laser create counter-propagating acoustic plane wave packets in the medium by electrostriction. Illumination of these wave packets with a second long-pulse probe laser, at frequency ΩL, generates a Bragg-diffracted signal beam that consists of two overlapped and co-propagating (but distinct) components. These two components are distinguished by their different Doppler shifts, ±∆ω, which are determined by the counter-propagating geometry of the two acoustic wave packets. The beating together of the two components, at frequencies ΩL ±∆ω, produces a modulation of this Bragg-diffracted signal beam at frequency 2 ∆ω. If the sound wave reciprocal wavelength is ∆k (i.e., wave vector difference of the two pump beams) and bold-faced quantities denote vectors, the two Doppler shifts are ∆ω = ∆k• (±V S), where ±V S are the velocities of the two counter-propagating wave packets and |±V S| = the speed of sound. Measurement of the beat frequency 2 ∆ω, with the known grating wavelength 1/∆k, yields the sound speed. Temperature T is also determined because T ∝√V S.If the medium is in motion at velocity V F (assumed to be parallel to ∆k and V S) the frequencies of the signal beam are shifted to ΩL + ∆k• (V F±V S), but the difference frequency and the beating are unchanged.The bulk fluid motion at velocity V F is also readily obtained from LITA. Walker et al. [10], have demonstrated LITA velocimetry using a single-mode probe laser and etalon to detect the shift due to fluid flow in the frequency domain. Here we use an approach[11], which does not require a narrow band, frequency-stable probe source or a high-finesse etalon. We introduce a local oscillator beam at the probe frequency ΩL, of suitable intensity, that is collinear with the diffracted signal beam. The detected signal then shows modulation at three frequencies: 2 ∆ω and ∆ω±(∆k•V F). The measured frequency at 2 ∆ω again gives V S and T, while V F is found from the difference in frequency 2 ∆ω′ between the other two components, where ∆ω′ = ∆k •V F. The signal is digitized, and the frequencies are extracted either by spectral methods (Prony’s method) or by Levenberg-Marquardt fitting in the time domain. Thus V S = ∆ω / ∆k, T ∝√V S, and V F = V S ∆ω′ / ∆ω = ∆ω′ / ∆k. Sound speed, temperature, and one component of fluid velocity are determined simultaneously in a measurement time of about 1 µs.3. Experimental Apparatus. The experimental setup is shown in the schematic of Fig. 1. Two 7-ns pump pulses (532 nm) are derived from a single laser beam (not shown) and crossed at 2 θ = 0.9 deg, making sure that longitudinal coherence between the two beams is maintained at the crossing point. The ~10 µs probe pulse (750 nm), derived from a second laser, traverses the crossing point at the Bragg phase-matching angle of φ = 0.6 deg. Both lasers operate at 10 Hz, however LITA data is acquired at 1 Hz. Peak pulse intensities are about 7 MW and 3 kW for the pump and probe beams, respectively. A fraction of the 750-nm beam is split off (not shown) from the probe to use as the local oscillator (LO) beam, which is directed exactly along the path expected for the Bragg-diffracted LITA signal. A beam block for both ofthe pumps and the probe reduces scattered light into the detector. The sample volume, defined by the overlap volume of the crossing of the probe and LO input beams, is ellipsoidal, about 1 by 15 mm, with the longer dimension collinear with the optic axis and perpendicular to the flow direction. The long axis of the ellipsoid is located 5 mm downstream from the exit plane of a subsonic jet. A variable-speed jet exhausting into the room air (with a 5 by 35-mm rectangular cross section nozzle) was used to produce a range of uniform flow velocities over the sample volume, by varying the backing pressure of the jet.FIG. 1. Schematic of the setup used to make temperature and heterodyned velocity measurements with LITA.A photomultiplier tube is used to monitor the time dependent signal intensity that results from both the Bragg-diffracted signal and the LO that propagate at angle φ through the aperture in the blocking mask of Fig. 1. The signals are digitized with 500-MHz-bandwidth oscilloscopes. As in Ref. [7], a second LITA signal is simultaneously generated in a reference cell with known temperature to use as a reference signal to normalize out small pulse-to-pulse fluctuations in the beam crossing angles. This normalizing correction improved the quality of data in [7], but turned out to be negligible compared to random errors in the current experiments.A typical example of the digitized LITA signal (noisy curve) obtained for a single laser pulse with a nonzero flow velocity is shown in Fig. 2a. We first apply Prony’s method to the data of Fig. 2a to determine the important frequency components. We use the result from Prony’s method as the initial guess in a nonlinear least squares fitting routine. The result of this Levenberg-Marquardt fitting routine (smooth curve) is then taken as the final result for the values of the beat frequencies. The difference between the fit and the data (noisy curve) is shown (with the same scale as used for the upper trace) in the lower trace of Fig. 2a. Fig. 2b shows three peaks in the spectral transform of the temporal data of Fig. 2a. The value of 2 ∆ω is given by the frequency of the right-most peak, while the value of 2 ∆ω′ is given by the frequency difference of the two left-most peaks. In the example of Fig. 2, the airflow speed is about 25 m/s.With our geometry (the probe beam is larger than the pump beam at the crossing point), to obtain the degree of precision reported here, we have to include the motion of the density gratings in the model that we fit to the data. Because the detector is fixed, the propagation of small acoustic wave packets across the larger probe beam changes the scattering angle and the magnitude of the Doppler shift. In other words, there is a very small frequency chirp (not obvious to the eye in Fig. 2a, but detectable in the fitting) over the two microseconds that we observe the LITA signal. The differences between the fits and the data are significantly reduced, especially at low velocities, when we include this grating motion (or frequency chirp) in the fitting model.FIG. 2. In the upper trace of (a), a single-laser-shot heterodyned LITA example of the temporal profile shows the data [noisy curve] and a fit to the data [smooth curve]. The lower trace of (a) shows the difference between the fit and the data. In (b) the corresponding spectral transform of the data is shown. Velocity, Mach number and temperature are obtained from the frequencies of the three peaks.4. Results. A comparison between LITA and pitot tube velocity measurements is shown in Fig.3. In Fig. 3a, LITA velocity is plotted and, in Fig. 3b, the percentage difference (100 ∗ [V LITA – V pitot] / V pitot) is plotted - both versus pitot-tube velocity. Pitot-tube data is acquired simultaneously with the LITA data, but 4 mm downstream from the LITA measurement location (i.e., 9 mm downstream of the jet exit plane). Placing the LITA beams closer to the pitot tube would have scattered laser light that would have degraded the LITA signal-to-noise ratio. After blocking the LITA laser beams, we have measured the velocity gradient between these two measurement locations with the pitot tube. The magnitude of the velocity gradient, between the two measurement locations, was about 1-2% of the velocity at the low-end(20 m/s) of the velocity range and about 10 times smaller at the high-end (150 m/s). The comparison between the LITA and pitot-tube measurements shown in Fig. 3 has been corrected for the velocity gradient between the 5 and 9-mm positions. The differences between LITA and pitot-tube measurements vary from 0.2% near 150 m/s to 2% near 20 m/s. Each LITA velocity and velocity difference data point shown is the average of about ~ 50 laser shots. Error bars are omitted for clarity. In part (a), one standard deviation of the population of the LITA data is about the size of the symbol. For the differences shown in part (b), one-standard deviation of the population for each averaged point varies from ± 1% at 150 m/s to ± 4% at 20 m/s. For both parts of Fig. 3, the errors in the mean values are about √50 ≈ 7 times smaller.FIG. 3. LITA (a) velocity and (b) the difference between LITA and a pitot tube versus the pitot-tube velocity. Pitot tube data are acquired simultaneously with the LITA data. Each velocity and velocity difference point is the average of ~ 50 laser shots.In Fig. 4a, LITA temperature (solid circles), for the same run as in Fig. 3, is plotted versus pitot-tube velocity, along with an isentropic flow calculation (open diamonds) that is based on the measured stagnation temperature inside the plenum of the flow generator and the measured pitot-tube Mach number. In Fig 4b, the percentage difference (100 ∗ [T LITA – T calc] / T calc) is plotted. The differences are 0.3% or less. Each temperature and difference data point shown is the average of ~ 50 laser shots. Error bars are also omitted from Fig. 4 for clarity. In part (a), one standard deviation of the population for each LITA data point is typically ± 1.1 K or ± 0.4%. Again, the one-standard deviations of the means are about √50 ≈7 times smaller.FIG. 4. LITA (a) temperature measurements (solid circles) obtained from the same data set as used in Fig. 3, plotted as a function of pitot-tube velocity, and compared to an isentropic-flow calculation (open diamonds). The differences between LITA data and calculation are shown in (b).5. Conclusion. In summary, non-resonant LITA has been demonstrated with heterodyne detection to measure simultaneously temperature and a single component of velocity at a single point in a free-expansion atmospheric-pressure air jet. The agreement of the LITA velocity with simultaneous pitot-tube measurements varies from 0.2% at 150 m/s to 2% at 20 m/s. The LITA temperature measurements agree with the isentropic expansion model within about 0.3% for the temperature range 285-295 K. The good agreement between LITA and these established methods suggests that nonresonant LITA can be reliably used for simultaneous flow velocimetry and thermometry for these flow conditions.Acknowledgements. We gratefully thank D. Morris (Kennedy Space Center) for contributing to this work.REFERENCES[1] S. W ILLIAMS, L.A. R AHN, P.H. P AUL, J.W. F ORSMAN, AND R.N. Z ARE, Laser-induced ThermalGrating Effects in Flames, Opt. Lett. 19 (1994), pp. 1681-1683.[2] E.B. C UMMINGS, Laser-induced Thermal Acoustics: Simple Accurate Gas Measurements, Opt,Lett. 19 (1994), pp. 1361-1363.[3] M.A. B UNTINE, D.W. C HANDLER, AND C.C. H AYDEN, Detection of Vibrational OvertoneExcitation in Water Via Laser-Induced Grating Spectroscopy, J. Chem. Phys. 102 (1995), pp.2718-2726.[4] E.B. C UMMINGS, H.G. H ORNUNG, M.S. B ROWN, AND P.A. D EBARBER, Measurement of Gas-phase Sound Speed and Thermal Diffusivity Over a Broad Pressure Range Using Laser-induced Thermal Acoustics, Opt. Lett. 20 (1995), pp. 1577-1579.[5] A. S TAMPANONI-PANARIELLO, B. H EMMERLING, AND W. H UBSCHMID, TemperatureMeasurements in Gases Using Laser Induced Electrostrictive Gratings, Appl. Phys. B 67 (1998), pp. 125-130.[6] M.S. B ROWN AND W.L. R OBERTS, Single Point Thermometry in High-Pressure Sooting,Premixed Combustion Environments, J. Propulsion Power 15 (1999), pp. 119-127.[7] R.C. H ART, R.J. B ALLA, AND G.C. H ERRING, Nonresonant Referenced Laser-Induced ThermalAcoustics Thermometry in Air, Appl. Opt. 38 (1999), pp. 577-584.[8] R.C. H ART, R.J. B ALLA, AND G.C. H ERRING, Optical Measurement of the Speed of Sound in AirOver the Temperature Range 300-650 K, J. Acoust. Soc. Am., accepted for publication.[9] S. S CHLAMP, E.B. C UMMINGS, AND T.H. S OBOTA, Laser-Induced Thermal-Acoustic VelocimetryWith Heterodyne Detection, Opt. Lett. 25 (2000), pp. 224-226.[10] D.J.W. W ALKER, R.B. W ILLIAMS, AND P. E WART, Thermal Grating Velocimetry, Opt. Lett. 23(1998), pp. 1316-1318.[11] A.A. M AZNEV, K.A. N ELSON, AND J.A. R OGERS, Optical Heterodyne Detection of Laser-InducedGratings, Opt. Lett. 23 (1998), pp. 1319-1321.。