不同标记荧光基团的稳定性、半衰期介绍说明

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Color code legene:

Red = Proprietary; Pink = Discontinuation; Green = Anecdotal; Blue = Anything else customers will not see Custom Primers – All Modifications

TABLE OF CONTENTS

PRODUCT DESCRIPTION

SHIPPING CONDITIONS

STORAGE CONDITIONS

STABILITY

QC SPECIFICATIONS

PROTOCOL & APPLICATION NOTES

Modification/scale/purification

Manufacturing Details

Fluorescein

Rhodamine

HEX/TET/FAM

Phosphate

Biotin

Amine

Gateway

Alexa

Other dyes

Alkaline Phosphatase

Horse Radish Peroxidase

Phosphorothioate

“Fully-Phosphorothioated" Custom Primers

Aldehyde

Acridine

Thiol

Delivery Schedule

OligoPerfect Designed Primers

3' Modifications of Oligos

Reconstitution Protocol

A260/A280 ratio of the oligo

Oligo Visualization

Troubleshooting

“Custom Custom” modifications

List of current technology limitations

COMPETITOR INFORMATION

ALTERNATE PRODUCTS & COMPATIBILITY

PRODUCT DOCUMENTATION

REFERENCES

PRODUCT NAME & CATALOG NUMBER

COMPONENTS

DISCONTINUATION NOTICE

ASSOCIATED PRODUCTS

PRODUCT QUALITY ISSUES

Returning Primers

LICENSING

INTERNAL CONTACTS

PRODUCT DESCRIPTION

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Custom DNA Primers are synthetic oligonucleotides with specified sequence made to order for use in a variety of applications from PCR and sequencing to probes for gene detection. Custom DNA Primers are available as standard deoxynucleotides, modified bases, 5´ modified nucleotides (including fluorescent dyes, enzyme conjugates), and S-oligos for antisense studies. Five scales and four purity levels are available.

A unique and proprietary technology and an optimized production process is used to deliver quality Custom Primers, quickly and efficiently. The process includes easy ordering, linkage to integrated workstations for guaranteed accuracy, high-throughput synthesis, monitoring by a series of stringent quality control steps, useful product packaging, and overnight shipping for rapid, reliable delivery. Custom oligonucleotides are synthesized on automated, proprietary DNA synthesizers using standard cyanoethyl phosphoramidite chemistry. Consistent oligonucleotide quality is ensured by in-process trityl monitoring and quality control analysis. A comprehensive Certificate of Analysis accompanies each order, indicating quantity, molecular weight, extinction coefficient, sequence, and melting temperature.

DNA oligos can be ordered in tubes or plates:

•Tubes:

o Standard tube with cap (Scientific Specialties Inc. (SSI) 2680-00 (tube), 2001-00 (cap)). Volume is 2000µl •Plates:

o96-wells:

PCR shallow 96-well plate (Abgene AB-0800): 200µl max volume, 180µl working volume; plate code 96N – 180. Shape of wells: V

Standard shallow 96-well plate (Corning Costar 3359): 360µl max volume, 250µl working volume;

plate code 96B – 250. Shape of wells: U/round. OLD SHALLOW PLATE

Standard medium 96-well plate (Abgene AB-0765): 800µl max volume, 650µl working volume; plate code 96L – 650. Shape of well: conical.

Cluster tube screwtop 96-well plate (Matrix ScrewTop TrackMates 3742, ): 1000µl max volume, 750µl working volume; plate code 96D – 750. Shape of well: Unknown.

SPECIAL I

Standard deep 96-well plate (Thompson Instruments, Oceanside distributor, part number 951651B): 1200µl max volume, 800µl working volume (equivalent to Abgene AB-0564); plate code 96C – 800.

Shape of well: U/round. OLD DEEP WELL PLATE

Cluster tube capmat 96-well plate (Matrix TrakMates 3791, ): 1400µl max volume, 850µl working volume; plate code 96J – 850. Shape of well: V. SPECIAL II o384 wells:

Standard 384-well plate – Thompson Instruments (Oceanside distributor) part number 931504B, 120µl max volume, 75µl working volume (equivalent to Abgene AB-0781); plate code 384E – 75. Shape of

well: pyramidal.

Microarray 384-well plate (Genetix X7020): 65µl max volume, 45µl working volume; plate code 384F – 45. Shape of well: flat.

o Seals and caps:

Heat Seal – Thompson Instruments (Oceanside distributor) part number 899406 (equivalent to Abgene AB-0745)

Adhesive Seal – Thompson Instruments (Oceanside distributor) part number 899405-1

Caps – Matrix ScrewTop caps 4472, for use with cluster tube screwtop 96-well plate, 96 caps required for each plate

Capmat – Matrix SepraSeal Cap Mats 4463, for use with cluster tube capmat 96-well plate, one mat required for each plate

SHIPPING CONDITIONS

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