PROPERTIES FOR MODULATION SPECTRAL FILTERING

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测定mgf2薄膜的复折射率光谱的英文

测定mgf2薄膜的复折射率光谱的英文

测定mgf2薄膜的复折射率光谱的英文The Characterization of the Refractive Index Spectrum of MgF2 Thin FilmsThe optical properties of thin-film materials have become increasingly important in various technological applications, ranging from optoelectronic devices to optical coatings. Among the numerous thin-film materials, magnesium fluoride (MgF2) has gained significant attention due to its unique optical characteristics, such as a wide transparent spectral range, low refractive index, and excellent chemical stability. Accurate determination of the refractive index spectrum of MgF2 thin films is crucial for the design and optimization of optical components and systems.In this study, we aim to present a comprehensive characterization of the refractive index spectrum of MgF2 thin films. The refractive index of a material is a fundamental optical property that describes the speed of light propagation within the material. The refractive index can be wavelength-dependent, leading to a refractive index spectrum, which is essential for understanding the optical behavior of thin-film materials.To achieve this goal, we employed a combination of experimental techniques and theoretical analysis. The MgF2 thin films were deposited on glass substrates using a well-established deposition method, such as thermal evaporation or sputtering. The thickness of the films was carefully controlled to ensure the accuracy of the refractive index measurements.The refractive index spectrum of the MgF2 thin films was determined using a spectroscopic ellipsometry technique. Ellipsometry is a non-destructive optical characterization method that measures the change in the polarization state of light upon reflection from the sample surface. By analyzing the ellipsometric data, the refractive index and other optical properties of the thin films can be accurately determined.The measurement process involved placing the MgF2 thin-film sample in the ellipsometer and collecting the ellipsometric data over a wide range of wavelengths, typically from the ultraviolet to the near-infrared region of the electromagnetic spectrum. The collected data were then analyzed using appropriate optical models and numerical algorithms to extract the refractive index spectrum of the MgF2 thin films.To ensure the reliability and accuracy of the refractive index data, several factors were considered during the measurement andanalysis processes. These factors include the surface roughness of the thin films, the potential presence of anisotropy, and the influence of the underlying substrate. Appropriate mathematical models were employed to account for these factors and obtain a accurate refractive index spectrum.The results of the study revealed the detailed refractive index spectrum of the MgF2 thin films over the measured wavelength range. The refractive index was found to exhibit a strong wavelength dependence, with the value decreasing as the wavelength increased. This behavior is consistent with the dispersion characteristics ofMgF2, which is known to have a low refractive index and high transparency in the visible and near-infrared regions.Furthermore, the study also investigated the potential effects of film thickness and deposition conditions on the refractive index spectrum. By varying these parameters, the researchers were able to understand the relationship between the thin-film properties and the resulting optical characteristics. This knowledge can be valuable for tailoring the optical performance of MgF2 thin films for specific applications.The findings of this study contribute to the existing understanding of the optical properties of MgF2 thin films and provide a reliable reference for the refractive index spectrum. This information is crucialfor the design and optimization of various optical components and devices that utilize MgF2 as a key material, such as antireflective coatings, optical filters, and optical waveguides.In conclusion, this comprehensive study on the refractive index spectrum of MgF2 thin films offers valuable insights for researchers and engineers working in the field of optical thin-film technology. The accurate characterization of the refractive index spectrum presented here can facilitate the development of advanced optical systems and devices that harness the unique optical properties of MgF2.。

多糖主链及支链组成的测定方法

多糖主链及支链组成的测定方法

多糖主链及支链组成的测定方法英文回答:Determining the composition of a polymer main chain and side chains involves several analytical techniques. One common method is spectroscopy, which includes techniques like nuclear magnetic resonance (NMR) spectroscopy and infrared (IR) spectroscopy.NMR spectroscopy is a powerful technique for determining the structure of organic compounds, including polymers. It provides information about the connectivity and chemical environment of atoms in a molecule. By analyzing the NMR spectrum of a polymer, we can identify the main chain and side chain components. For example, if we have a polymer with a main chain of polyethylene and side chains of polypropylene, the NMR spectrum would show characteristic peaks for the different types of protons in each component.IR spectroscopy is another valuable technique for polymer analysis. It measures the absorption of infrared light by the polymer sample, providing information about the functional groups present. Different functional groups have characteristic absorption bands, allowing us to identify the main chain and side chain components. For instance, if we have a polymer with a main chain of polyvinyl chloride and side chains of polyethylene glycol, the IR spectrum would exhibit absorption bands corresponding to the C-Cl bond in the main chain and the ether group in the side chains.In addition to spectroscopy, another method for determining the composition of a polymer main chain and side chains is chromatography. Gel permeation chromatography (GPC) is commonly used for this purpose. GPC separates polymers based on their size, allowing us to determine the molecular weight distribution and composition of the polymer. By comparing the GPC results of a polymer sample with known standards, we can identify the main chain and side chain components. For example, if we have a polymer with a main chain of polystyrene and side chains ofpoly(methyl methacrylate), GPC would show two distinctpeaks corresponding to the different molecular weights of the main chain and side chains.Overall, a combination of spectroscopy techniques like NMR and IR, along with chromatography techniques like GPC, can be used to determine the composition of a polymer main chain and side chains. These methods provide valuable information about the structure and properties of polymers, aiding in their characterization and development.中文回答:确定聚合物主链和支链的组成涉及多种分析技术。

MODO_Manual

MODO_Manual

MODO User Manual, Version 3© 2006 by ReSe. All rights reserved.This manual, as well as the software described in it, is furnished under license and may only be used or copied in accordance with the terms of such a license. The information in this manual is furnished for informational use only, is subject to change without notice, and should not be construed as a commitment by ReSe.Software and manual are completely made in Switzerland.MODO software authored and produced by ReSe Applications Schläpfer.Year of the first publication of manual: 2006; place of publication: Wil (SG), Switzerland. MODO user manual authored by Daniel Schläpfer, Dr. sc. nat. and Daniel Odermatt, MSc.Front cover:Simulation of transmitance values using the MODO software.Table of Contents Table of ContentsTable of Contents (3)Chapter 1:Introduction1.1Goals of MODO (5)1.2Functionality (6)1.3Limitations (7)1.4Future Extensions (7)1.5Organisation of this Manual (7)1.6Installation of the MODO Software (8)Chapter 2:Background Information2.1MODTRAN and MODO Integration (11)2.2Procedures (13)2.2.1Data Extraction (13)2.2.2Convolution (14)2.3File Descriptions (15)2.3.1Band Model Files (15)2.3.2Solar Irradiance Spectra (16)2.3.3Sensor Response Spectra (17)2.3.4Surface Reflectance Files (17)2.3.5Outputs (18)2.4Demo Data (19)2.4.1Spectral Libraries (19)2.4.2Tape5s (19)Chapter 3:Workflow Examples3.1MODTRAN Setup (21)3.2At-sensor Radiance Simulation (23)3.3Simulation of Atmospheric Signatures (26)3.4Simulation of Sensititivity Series (27)3.5Evaluation of Sensor Specifications (29)3Table of ContentsChapter 4:Functions Reference Guide4.1Generic Menu Elements (31)4.1.1The MODO main window (31)4.1.2Help System (31)4.1.3Text Editing (32)4.1.4Selecting Albedo Spectra (33)4.1.5Selecting Lambertian Albedo Spectra (34)4.1.6Plotting (35)4.1.7Session Management (37)4.2Menu: File (37)4.3Menu: Edit (41)4.4Menu MODTRAN4: Setting up a tape5 (44)4.5Menu: MODTRAN4 (53)4.6Menu: Calculate (65)4.7Batch Processing (70)References (71)Index (75)4Introduction Chapter 1 Chapter 1:IntroductionThe radiative transfer code MODTRAN1, version 4 [2] [3] has been established as de-factostandard for the simulation of imaging spectrometry data and for quantitative modelling of thesignal at the sensor level. The original interface of MODTRAN consisting of ASCII-file basedinputs leads often to misunderstandings and mistakes in such analyses. Almost every frequentuser of MODTRAN has therefore some tools available to ease the setup of the inputs.MODO is a MODTRAN interface, implemented by ReSe Applications Schläpfer under sup-port of the Remote Sensing Laboratories (RSL) of the University of Zurich. It is maintainedand distributed by ReSe Applications Schläpfer. MODO includes an almost complete transla-tion of the logical structure and the parameters of the input ‘tape5’ as well as utilities for theextraction and convolution of radiation component spectra.Hereafter, a short overview of the software is given. Background information, workflowdescriptions, and a functions reference can be found in the subsequent chapters of this manual.1.1Goals of MODOThe major goal of MODO is to ease the use of MODTRAN by providing a graphical userinterface (GUI) for the creation of the input files as well as for the treatment of the outputswith respect to hyperspectral remote sensing. The efforts resulted in the MODO (‘MODT-RAN Organizer’) concept. MODO is a graphical front-end to the MODTRAN4 radiativetransfer code. Its basic functionality is the creation and translation of files of the type ‘tape5’ or‘.tp5’. The subsequent processing of output spectra, regarding extraction, conversion and plot-ting, can then be done in the same working environment. Additional functionalities allow theconvenient creation of sensitivity analysis series and the convolution of spectra to hyperspectralband characteristics.1.Licensed from the United States of America under U.S. Patent No. 5,315,513.5Chapter 1Introduction 1.2FunctionalityMODO version 3 includes the following features:•Import/export of MODTRAN4 tape5 ASCII control files•Creation and dealing with multiple run tape5s•Editing of own, customized atmospheres•Import/export of ground reflectance spectra including support for adjacency effect•Support for ENVI TM spectral libraries•Sensitivity analysis through parameter series•Series of reflectance spectra•Direct call of MODTRAN for Windows and UNIX/Linux/OSX•Includes original executables of MODTRAN v3r1 for Windows and UNIX/Linux/MacOSX (PowerPC)•Extraction of radiance/transmittance components from MODTRAN output (e.g. tape7)•Extraction of solar flux data from MODTRAN ‘.flx’ files•Plotting of standard MODTRAN outputs (tape7/flux)•Convolution of outputs to hyper- (gaussian response) and multispectral sensors•Eased sensor simulation with a broad collection of response functions for both airborneand spaceborne optical and thermal instruments•Helper applications for visibility determination and solar angles calculation•Direct online help for each GUI panel and this electronic user manualThe MODO interface design is implemented in view of improving the reliability of simula-tions for optical remote sensing instruments. This end-to-end solution starts with inclusionand selection of surface reflectance functions from spectral libraries. Second, the atmosphericparameters most critical to the radiative transfer are to be defined, and third, the componentsof the at-sensor radiance shall be produced directly for specific sensor response functions. Thepre-selection of relevant situation parameters is done on experience in various application area.The integration of the given principles has lead to a comprehensive GUI for setting upMODTRAN runs in an efficient manner.6Introduction Chapter 1 1.3LimitationsMODO has been developed in view of remote sensing data analysis and simulations. It is lim-ited to the following restrictions:•MODO is an expert simulation tool which (still) requires some knowledge about radiativetransfer simulation principles.•BRDF functionality of MODTRAN4 is not supported.•Multi-dimensional look up table generation is not easily feasible through the interface.•No import functions for user defined aerosol phase functions and standard radiosondeprofiles are available.1.4Future ExtensionsThe MODO application is under continuous improvement. The following features areoptions to be potentially included in future versions of the software (depending on demand):•Support for BRDF input•Input of standard radiosonde profiles•Input of MISR aerosol models•Sun photometer data analysisSuch features are implemented based on specific requests of licensed end users. Please contactReSe, if you have new ideas or wishes to the software or if you’d like to contribute suited IDL-based tools to be included in the processing system.1.5Organisation of this ManualThis manual is organized as follows:•This Chapter ’Introduction’.•The second Chapter ’Background Information’ gives some explanations about specifics ofthe MODO application.•The Chapter ’Workflow Examples’ gives guidelines how to work with MODO interac-tively. It summarizes tips for working with standard sensor data and how to deal with spe-cial cases.7Chapter 1Introduction8•The Chapter ’Functions Reference Guide’ describes every function of the MODO userinterface and the usage of the interface functions. Finally, the bibliographic references aswell as an index of topics can be found in the Appendix.Some conventions in the manual:•Menu commands are given as >File:Restore Status p.39<, with a link to the description page.•Batch routines and calls on the IDL prompt are written in monotype,e.g., modo,/norun.Please read the warning texts which are marked by warning sings on the side-bars carefully.1.6Installation of the MODO SoftwareThe distribution of MODO includes platform-specific MODTRAN4 exectuables, compiledfrom the original MODTRAN code and compatible to all current operating systems (Solaris/Linux/MacOSX/Windows). The system requirements are:•IDL 6.2 or higher or the free IDL Virtual Machine (RSI Inc.)•Solaris, Linux (x86), MacOSX (PowerPC) or Windows operating system•High processing power for MODTRAN runs•Screen size at least 1024x768 pixels•100 MB disk storageThe MODO application installer is available from www.rese.ch/download.html . If you don’thave access to an official IDL license, the IDL Virtual Machine is available as free distributiondirectly from ITTVIS, through /idlvm . The MODO installation process is asfollows:1)Install the IDL virtual machine following the installation instructions provided by RSI(this step is void, if you have IDL/IDL VM/ or ENVI developer installed).2)Double click the file modo_installer.sav (on Windows) or enter on Unix/Linux/MacOSX:idl -vm="modo_installer.sav".3)Please follow the instructions as displayed during the installation process.Introduction Chapter 194)For licensing, go to the help menu after starting MODO and choose ‘Identify’ in the menu>Help:License<. Please email the displayed outputs of this job together with your completeaddress and affiliation. You will then receive a license key file within a few days. Let usknow if you need any further assistance or product information.A free 30 days, fully functional evaluation license key may be issued upon request. After expi-ration of the license, you will need to acquire a license as described above or on the ReSe home-page. If not, you will still be able to run MODO in demonstration mode, which allows thehandling of MODTRAN outputs, but does not support running MODTRAN and MODT-RAN series.Chapter 1Introduction 10Chapter 2:Background InformationThis chapter summarizes some background information about the MODO/MODTRAN sim-ulation environment.2.1MODTRAN and MODO IntegrationThe MODTRAN code as provided by the Air Force Geophysics Laboratory (AFGL) is writtenin the FORTRAN computing language. It is handled by rigidly formatted ASCII input files.The tape5 is used for the definition of the atmosphere and the geometry, while the file‘spec_alb.dat’ (e.g.) defines the background reflectance characteristics. Other optional inputfiles concern the solar irradiance or the spectral band model. The direct handling of these filesis very sensitive and requires experience with the code. This also bears the danger of introduc-ing errors in at-sensor data simulations.The interface is based on the IDL [14] programming language which has been established asde-facto standard for hyperspectral image processing. The design has been optimized forresearch applications and thus does not support high degrees of automatism, avoiding ‘blackbox’ mechanisms. The MODO concept as shown in Figure 2.1 is based on the standard dis-tribution of MODTRAN by interfacing with the inputs ‘.tp5’ and ‘spec_alb.dat’, and evaluat-ing the outputs ‘tp7’ and ‘.flx’.One core interface function of the procedure is the tape5 editor window (>Modtran4:SetupTape5 and Run p.53<). It allows to set most of the input parameters using pull-down menusinstead of manually editing the rigidly formatted ASCII file. Logics within the tape5 are con-sidered, such that if, e.g., the transmittance mode has been selected it is not possible to set theirradiance source options. Sub-interfaces will pop up for supported special functions such asthe import of user defined atmospheres, the selection of the surface reflectance, or the defini-tion of the four standard aerosol layers. The interface is grouped in the same way as in the orig-inal tape5 to be consistent with the documentation as provided with MODTRAN. If one ormore parameters shall be varied, the setup of multiple run tape5s has proven to be very useful.Each run within such tape5s can be accessed, edited, or deleted individually by browsingthrough the tape5. Some dedicated save options help to keep various tape5s organized.The inclusion of surface reflectance spectra has become of high importance for modelling at-sensor radiance values for known targets. An interface has therefore been included for import-ing reflectance data into MODTRAN from ENVI [10] spectral libraries or ASCII reflectancefiles. The spectra can afterwards be selected for the target as well as for the background, if adja-cency effects shall be studied (>Edit:Import Spectra p.41<). Alternatively, an even more stream-lined function (>Modtran4:Reflectance Series p.57<) is included for direct simulation of at-sensorsignals based on surface reflectance libraries.The startup of the original MODTRAN executable is managed by a child process from withinMODO. The code has been slightly adapted in order to allow to use MODTRAN from what-ever directory the tape5 has been saved to. Additional interfaces are included for the followingtasks:•Plotting of the spectral output (tape7 or solar flux)•Calculation of solar angles for time and date•Save/restore of settings•Extraction of single spectra from the whole output•Parameter and reflectance series simulation•Convolution to hyperspectral (Gaussian) channel characteristics MODO MODTRAN tape5spec_alb.dat tape7solar flux(fortran)input preparationMODO reflectanceoutput evaluationradiance Lconvolved Lplotssensor response Figure 2.1:Integration of the MODTRAN standard code with the MODO interface.•Export of radiance spectra to ENVI spectral libraries All these utilities have been developed in support of a flexible handling of the MODTRANinputs and outputs for a fast simulation of at-sensor radiance values. They are described in detail in Chapter 4 on Page 31.2.2ProceduresMODO by itself is only an interface to MODTRAN. The MODTRAN code has been des-ribed in detail elsewhere [2] [3] [8], whereas a full description is available commerciallythrough . The functionality which is specific to MODO is related to dataextraction and convolution, because the standard wavenumber reference of MODTRAN is[cm -1]. In VIS/NIR spectrometry (and optical remote sensing) the standard wavelength refer-ence is [nm] and therefore, some conversion is required.MODTRAN by itself also offers a unit conversion and convolution option which is fully inde-pendent from the options as implemented within MODO, due to operational disadvantagesof the MODTRAN implementation.2.2.1Data ExtractionIn normal cases, the total at-sensor radiance is the main output component to be read from theMODTRAN outputs. Other components such as the path scattered radiance, specific trans-mittance values or the solar irradiation, are of specific interest for atmospheric applications andcorrection routines as well as for validation of the cross sensitivity of the simulated spectra toatmospheric influences. MODO reads the components from the outputs and converts them toSI standard units [W/(m 2 sr nm)] from the original units being [W/(cm 2 sr cm -1)]. This con-version is based on the well-known relationship between wavelength and wavenumber :, (2.1)The wavenumber is converted to its equivalent wavelength through the following relationship:.(2.2)The relation between the wavelength interval and the wavenumber interval is given by:λνλ1ν--=λnm []1νcm 1–[]---------------------107ν-------nm []==and .(2.3)The generic relation between the radiance per wavelength and the radiance per wavenum-ber is derived from the respective definitions:,and with (2.1): .(2.4)The unit conversion is derived as follows, where , and denote data values for the same radiance equivalents and the wavenumber value in inverse centimeters:.(2.5)The standard unit in [cm -1] is given as the original MODTRAN wavenumber reference which may be related closely to the energy levels of the simulated photons. But in imaging spectrom-etry and spectroscopy of the visible/near infrared part of the spectrum, the most common wavelength references are microns or nanometers. As the resolution of typical VIS/NIR imag-ing spectrometers is in the range of 1to 20 nm, it has been decided to select the wavelength in nanometers as generic reference for data simulation within MODO.(Compare function: >Modtran4:Extract Spectra p.59<.)2.2.2Convolution The MODTRAN data usually is derived in wavelength units using a triangular slit for convo-lution to the original band data. Since version 3.7 of MODTRAN, an option is included which allows the direct convolution of the MODTRAN outputs to sensor specific response functions.This option is not fully supported within MODO. A separate convolution function convolves extracted and possibly joined spectra to sensor characteristics using a Gaussian approximation of the sensor function or explicite response functions. This option leaves higher flexibility for research purposes if, e.g., the response function needs to be varied. The convolved radiance val-ues in a band are calculated as:,(2.6)d λ1ν2----d ν–=d νν2d λ–=L S λ,L S ν,L S ν,d φdAd Ωd ν---------------------=L S λ,d φdAd Ωd λ---------------------d φν2dAd Ωd ν---------------------==L S λ,L S ν,νL S λ,W m 2srnm -------------------ν2L S ν,W cm 1–()2cm 2sr cm 1–()------------------------------ν2L S ν,104W cm 1–()m 2sr ---------------------ν2L S ν,103–W m 2srnm -------------------===L i i L i L S λ()r i λ()λd ∫r i λ()λd ∫-------------------------------------L S λj ()r i λj ()∆λj j ∑r i λj ()∆λjj ∑------------------------------------------------≈=where is the spectral response function of the sensor’s band. A stepwise assumption istaken for the convolution if the number of raw data values is sufficient within the width ofthe spectral band. If the original resolution is not sufficient, a polynomial is calculated throughthe original data points for better approximation of the spectrum and summarizedthrough a number of interpolated data points, i.e:.(2.7)A minimal number of 2 data points within the range of the target bands is required for a suf-ficient calculation of the convolved data values in any case.(Compare function: >Modtran4:Extract Spectra p.59<.)2.3File DescriptionsThe data basis for the MODTRAN calculation is provided together with the MODTRANcode. MODO contains some additional data for more complete simulation posssibilities,which are described in Chapter 2.4 on Page 19. An overview over the files provided byMODTRAN and their locations within the installation as described in the originalMODTRAN4 user’s manual [2] is given below.2.3.1Band Model FilesThe variable ‘MODTRN’ in the 1st position in CARD 1 (see Table 4.1 on Page 47) selectsthe band model algorithm used for the radiative transfer, either the moderate spectral resolu-tion MODTRAN band model or the low spectral resolution LOWTRAN band model.LOWTRAN spectroscopy is obsolete and is retained only for backward compatibility. TheMODTRAN band model may be selected either with or without the correlated-k treatment.The values for band model determination in ‘MODTRN’ are given in Table 2.1.r j λ()j L s λj ()k 100=L i Poly L S λj ()()k r i λk ()∆λk k ∑r i λk ()∆λk k ∑----------------------------------------------------------------------≈Table 2.1:‘MODTRN’ band model options.‘MODTRN’ valuesBand model ‘T’, ‘M’ or blankMODTRAN band models ‘C’ or ‘K’MODTRAN correlated-k option (IEMSCT radiance modes only; most accurate but slower run time).‘F’ or ‘L’20 cm -1 LOWTRAN band model (not recommended except for quickhistoric comparisons).MODTRAN uses a default 1 cm-1 band model, but if variable ‘LBNAM’ in CARD 1A is set to ‘T’, the file name of a 5 cm-1 or 15 cm-1 band model will be read from variable ‘BMNAME’in CARD 1A2. MODTRAN will open the corresponding 1 cm-1, 5 cm-1 or 15 cm-1 Corre-lated-k data file when input variable ‘MODTRN’ equals ‘C’ or ‘K’.:•‘DATA/B2001_01.BIN’: The 1 cm-1 band model file is used if no other file is specified.The name of the accordant CK data file is hardwired to ‘DATA/CORK01.BIN’.•‘DATA/B2001_05.BIN’: The 5 cm-1 band model allows faster short-wave calculations.The name of the accordant CK data file is hardwired to ‘DATA/CORK05.BIN’.•‘DATA/B2001_15.BIN’: The 15 cm-1 band model allows fastest short-wave calculations.The name of the accordant CK data file is hardwired to ‘DATA/CORK15.BIN’.In MODO’s MODTRAN base widget described in >Functions:Setup Tape5 and Run p.53<, the alternative band models described above are selected by switching ‘1 cm-1 Standard’ in the sec-ond frame to ‘Special Bandmodel’. When calculating >Functions:At-Sensor Signal p.53<, a choice of band models is available in the first frame.2.3.2Solar Irradiance SpectraIf variable ‘LSUN’ in CARD 1A is set to ‘F’ or left blank, the default solar 5 cm-1 spectral res-olution irradiances are used (block data routine ‘sunbd_f’).If variable ‘LSUN’ in CARD 1A is set to ‘T’, and variable ‘LSUNFL’ in CARD 1A is set to ‘F’or left blank, the file named ‘DATA/newkur.dat’ is used.If both variables ‘LSUN’ in CARD 1A and ‘LSUNFL’ in CARD 1A are set to ‘T’, ‘SUNFL2’in CARD 1A1 is used to define the top of atmosphere (TOA) solar irradiance database accord-ing to Table 2.2.Table 2.2:Listing of solar irradiance databases defined by ‘SUNFL2’.‘SUNFL2’ values Solar irradiance database1 or blank The corrected Kurucz database is used (DATA/newkur.dat).2The Chance database is used (DATA/chkur.dat).3The Cebula plus Chance data are used (DATA/cebchkur.dat).4The Thuillier plus corrected Kurrucz data are used (DATA/thkur.dat).a file name A user-defined database residing in the file is used.The solar databases provided by MODTRAN are obtained from various sources [1] [6] [7][17] [18] [19] [20] [38] [39] [41].The user-defined file must be in a special form. The first line must contain a pair of integers.The first integer designates the spectral unit [1 for frequency in wavenumbers (cm-1); 2 for wavelength in nanometers (nm); and 3 for wavelength in microns (µm)]. The second integer denotes the irradiance unit [1 for Watts cm-2, 2 for photons sec-1 cm-1/nm; and 3 for Watts m-2/µm or equivalently milli-watts m-2/nm]. The subsequent lines contain one pair of fre-quency and irradiance entry per line. There is no restriction on frequency or wavelength incre-ments. However, data beyond 50’000 wavenumbers are ignored. If needed, data in the user-supplied file are padded with numbers from ‘newkur.dat’ so that the data encompasses the range of 50 to 50’000 wavenumbers.The user-defined file has a form that is different from ‘DATA/cebchur.dat’, ‘DATA/thkur.dat’, ‘DATA/newkur.dat’, and ‘DATA/chkur.dat’.2.3.3Sensor Response SpectraIf variable ‘LFLTNM’ in CARD 1A is set to ‘T’, CARD 1A3 is used to select a user-supplied instrument filter (channel) response function file. Whenever this option is used, the included file ‘CHANNELS.h’ should be reviewed to insure consistency between the ‘CHANNELS.h’parameters and the input response function file.Sample AVIRIS (‘DATA/aviris.flt’) and LANDSAT7 (‘DATA/landsat7.flt’) filter response functions are supplied with MODTRAN. MODO comes with additional sensor response data for a broad range of sensors, which are stored in the directory ‘sensor_resp’.For more detailed information on sensor response file formats, see >Functions:Plot Response Function p.38<.2.3.4Surface Reflectance FilesThe variable ‘SALBFL’ in CARD 4L1 contains the name of the input data file being used to define the spectral albedo. The default spectral albedo file ‘DATA/spec_alb.dat’ may be used or a user-supplied file. If a user-supplied file is specified, it must conform the following criteria, which are stated in the original ‘DATA/spec_alb.dat’:•Lines beginning with an exclamation mark ‘!’ are ignored. Comments after an exclamationmark are also ignored.•Each surface is defined by a positive integer label, a surface name, and its spectral data. Theinteger label and surface name must appear as a pair on a header line with the integer labelfollowed by a blank.•Header lines must not include a decimal point ‘.’ before an exclamation mark, and spectraldata must include a decimal point.•Spectral data is entered with one wavelength (in microns) and one spectral albedo per line,separated by one or more blanks. The spectral wavelengths for each surface type must beentered in increasing order. The spectral albedos should not be less than 0 or greater than1.•The first 80 characters of each line are read in.The variable ‘CSALB’ in CARD 4L2 defines the number or name associated with a spectral albedo curve from the ‘SALBFL’ file. There are currently 46 spectral albedo curves available in the default spectral albedo file ‘DATA/spec_alb.dat’.2.3.5OutputsThe standard MODTRAN output files tape6, tape7 and tape8 are described in >Modtran4:Setup Tape5 and Run p.44<.MODO generates additional output in columnar ASCII format, with file extension ‘.out’:•>Edit:Import Spectra p.41<: The imported data is written to a file with the input file’s headerinformation marked out with exclamation marks. If multiple spectra are selected, the spec-tra are vertically listed one after another with their specifications in a title row, followed bytwo columns containing reference wavelengths and radiance or reflectance values. Thisformat is not suitable as input for >File:Quick Plot p.38< or >Edit:Labels and Columns p.43<, asthey require an input with horizontally stored value columns referring to the same refer-ence wavelength column. Use >File:Edit Textfile p.38< and >Modtran4:Append Spectra p.64< toproduce ASCII files containing multiple spectra listed horizontally.•>Edit:Labels and Columns p.43<: The output ASCII file has the same row/column format asit is displayed in the editing widget. There are no comments marked out, but only one titlerow containing the column labels. The radiance or reflectance values for each spectrum arelisted horizontally, all referring to the same reference wavelength in the first column. Theoutput can be plotted in >File:Quick Plot p.38<.•>Modtran4:Extract Spectra p.59<: The output ASCII file has the same row/column format asoutputs from >Edit:Labels and Columns p.43<. There are no comments marked out, but onlyone title row containing the column labels. The radiance or transmittance values for eachspectrum are listed horizontally, all referring to the same reference wavelength in the firstcolumn. The output can be plotted in >File:Quick Plot p.38<.•>Modtran4:Append Spectra p.64<: The output ASCII file has the same row/column formatas outputs from >Edit:Labels and Columns p.43<. There are no comments marked out, butonly one title row containing the column labels. The radiance or reflectance values for eachspectrum are listed horizontally, all referring to the same reference wavelength in the firstcolumn. The output can be plotted in >File:Quick Plot p.38<.2.4Demo DataThe main purpose of the demo data that comes with MODO, is to help new users explorefunctions and limitations of MODO. But it may also be useful as input data for more experi-enced users, to perform test runs or compare . The data is stored in ‘/demo_data/spec_lib’ and‘/demo_data/tape5’.2.4.1Spectral LibrariesThe directory ‘/demo_data/spec_lib’ contains two additional spectral libraries from ATCORand S6 to complement the spectral data provided in MODTRAN. Their different propertiesare described in Table 2.3.2.4.2Tape5sThe directory ‘/demo_data/tape5’ contains a couple of predefined tape5s representing exem-plary parameter sets for different types of atmospheric situations. They serve as examples fordifferent simulation types processible in MODO and can easily be customized to new, user-Table 2.3:Properties of the spectral demo data provided with MODO.MODTRANATCOR S6File name‘spec_alb.dat’‘atcor_ASCII_lib.txt’‘atcor_lib.sli’ & ‘.hdr’spectra_6s.txt Number of surfaces4620 3 standard cases Surface types VegetationSoilUrbanArtificialSnowIceSeaVegetation Agriculture Concrete Sea Lake Vegetation Sand Lake Spectral resolution (mostly low)high high Spectral range (mostly large)300-2600 nm 350-2600 nm。

体内药分考试题目和答案

体内药分考试题目和答案

体内药分考试题目和答案①与常规药物分析相比,体内药物分析有哪些特点?体内药物分析(Biopahrmaceutical Analysis),是一门新兴学科,是药物分析的重要分支,也是现代药学的创新、延伸和进展。

体内药物分析旨在经过各种分析手段,了解药物在体内的数量与质量变化,获得药物动力学的各种参数、药物在体内的生物转化、代谢方式和途径等信息。

特点:a干扰杂质多:生物样品中含有的蛋白质、内源性物质和药物的代谢产物都会干扰分析测定,样品普通需通过分离、净化才干举行分析。

b样品量少(ng/ml —ug/ml),别易重新获得。

在测定前需要浓缩、富集。

C由于药物浓度低,对分析办法的灵敏度和专属性要求高。

d要求较快提供结果(临床用药监护,中毒拯救等)e要有能够举行复杂样品分析的设备。

f工作量大,测定数据的处理和结果的阐明别太容易。

g有时由于浓度太低,需要测定其缀合物及代谢产物。

②妨碍血药浓度的因素有哪些?当药物进入体内后,大多数药物借助血液分布到作用部位或受体部位,当血药浓度达到一定水平常,才干产生相应的药理效应。

药物进入体内到产生一定的血药浓度,要通过一系列的过程,包括汲取、分布、代谢、排泄,而这一系列过程会受到多种因素的妨碍,从而妨碍药物在体内的药理效应。

1)机体因素a生理因素(年龄、性不、妇女妊娠等)年龄:婴幼儿——肝、肾等脏器发育别全,妨碍汲取、分布、代谢、排泄,药动参数与成人别同。

老年人——机体各组织生理功能退化,胃酸分泌减少,血中白蛋白浓度下落,肝肾血流量减少,药酶活性下落。

性不:妇女因激素水平妨碍生理功能,在药物汲取、蛋白结合率,分布容积及代谢方面与男性有所别同。

2)病理因素胃、肠道疾病——妨碍汲取,肝脏疾病——妨碍代谢,肾脏疾病——妨碍排泄3)遗传因素(代谢酶活性差异)酶活性有先天差异,用药个体代谢有快型和慢型之分,如乙酰基转移酶4)药物因素a剂型因素药物的粒子大小、晶型、辅料、工艺等妨碍药物在体内的溶解度。

电感耦合等离子体发射光谱法的英文简称

电感耦合等离子体发射光谱法的英文简称

电感耦合等离子体发射光谱法的英文简称全文共3篇示例,供读者参考篇1Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) is a powerful analytical technique used in many scientific fields. This technique utilizes the high temperature of a plasma to atomize and excite samples for elemental analysis. ICP-OES provides high sensitivity, accuracy, and precision, making it a popular choice for analyzing trace elements in various sample types.The process of ICP-OES involves generating a plasma by applying a high-frequency radio frequency (RF) current to a flowing gas, typically argon. The intense heat of the plasma vaporizes the sample and excites the atoms to emit characteristic light at specific wavelengths. This emitted light is then dispersed by a spectrometer and detected by a charged-coupled device (CCD) detector. The intensity of the emitted light is proportional to the concentration of the element in the sample, allowing for quantitative analysis.ICP-OES is widely used in environmental monitoring, pharmaceutical analysis, forensic science, and materials science, among other areas. It can detect a wide range of elements, from alkali metals to rare earth elements, with detection limits as low as parts per billion. Additionally, ICP-OES can analyze multiple elements simultaneously, making it a fast and efficient tool for elemental analysis.Overall, ICP-OES is a versatile and reliable technique for elemental analysis, providing accurate and precise results for a wide range of sample types. Its high sensitivity and ability to analyze multiple elements simultaneously make it an essential tool in many research and industrial laboratories.篇2Title: ICP-OES: The Technique Behind Inductively Coupled Plasma Optical Emission SpectroscopyIntroductionInductively Coupled Plasma Optical Emission Spectroscopy, commonly abbreviated as ICP-OES, is a powerful analytical technique used for the quantitative analysis of elements present in a sample. This technique utilizes the principles of inductively coupled plasma (ICP) and optical emission spectroscopy (OES) toprovide accurate and precise measurements of the elemental composition of a sample. In this article, we will explore the fundamentals of ICP-OES and its applications in various fields.Principles of ICP-OESICP-OES operates by generating a high-temperature plasma consisting of ionized gas atoms by introducing a sample into an argon gas stream. The plasma is sustained by an induction coil, which induces an electric current that generates heat, forming a high-energy environment capable of atomizing and ionizing the sample components. As the atoms and ions return to their ground state, they emit light at characteristic wavelengths, which can be measured by a spectrometer to identify and quantify the elements present in the sample.Advantages of ICP-OESICP-OES offers several advantages over other analytical techniques, making it a preferred choice for elemental analysis in various industries. Some of the key advantages of ICP-OES include:- High sensitivity and detection limits: ICP-OES can detect elements at trace levels, making it suitable for a wide range ofapplications, including environmental monitoring and pharmaceutical analysis.- Multi-element analysis: ICP-OES is capable of analyzing multiple elements simultaneously, providing comprehensive information on the elemental composition of a sample.- Wide dynamic range: ICP-OES can analyze elements across a wide concentration range, from parts-per-billion to percent levels, making it suitable for diverse sample types.- Speed and efficiency: ICP-OES offers rapid analysis times, allowing for high sample throughput and increased productivity.- Minimal sample preparation: ICP-OES requires minimal sample preparation, saving time and reducing the risk of sample contamination.Applications of ICP-OESICP-OES is widely used in various industries and research fields for elemental analysis due to its versatility and accuracy. Some common applications of ICP-OES include:- Environmental analysis: ICP-OES is used for the analysis of trace elements in soil, water, and air samples to assess environmental contamination levels.- Geological analysis: ICP-OES is employed in the analysis of rocks, minerals, and ores to determine their elemental composition and identify valuable mineral deposits.- Pharmaceutical analysis: ICP-OES is used in the pharmaceutical industry for the analysis of drug formulations, determining the elemental impurities present in pharmaceutical products.- Food and beverage analysis: ICP-OES is utilized for the analysis of food and beverage products to ensure compliance with regulatory standards and assess product safety.ConclusionICP-OES is a versatile and reliable technique for elemental analysis, offering high sensitivity, multi-element capabilities, and rapid analysis times. With its wide range of applications in various fields, ICP-OES has become an essential tool for researchers, analysts, and industry professionals seeking accurate and precise elemental analysis. As technology continues to advance, ICP-OES is expected to play a key role in shaping the future of analytical chemistry and elemental analysis.篇3Inductively Coupled Plasma Emission Spectroscopy (ICP-ES) is a powerful analytical technique widely used in various fields including environmental monitoring, pharmaceutical analysis, and material science. This technique is based on the inductively coupled plasma (ICP) as the excitation source and the emission spectroscopy for detecting and quantifying elements present in a sample.ICP-ES offers several advantages over other analytical methods. Firstly, it provides a high sensitivity, allowing for the detection of trace elements at parts per billion or even parts per trillion levels. This makes ICP-ES ideal for analyzing samples with low concentrations of elements of interest. Secondly, ICP-ES has a wide dynamic range, enabling the simultaneous analysis of multiple elements present in a sample. This feature is particularly useful when analyzing complex samples containing a diverse range of elements. Additionally, ICP-ES offers excellent precision and accuracy, making it a reliable technique for quantitative analysis.The principle of ICP-ES involves the generation of ahigh-temperature plasma by inducing an electric current in a gas (typically argon) using a radiofrequency source. The plasma reaches temperatures of up to 10,000 Kelvin, causing the sampleto be atomized and ionized. As a result, the atoms and ions emit characteristic radiation when transitioning from excited states to ground states. The emitted radiation is then dispersed and detected by a spectrometer, allowing for the identification and quantification of elements based on their unique emission spectra.The use of inductively coupled plasma as the excitation source offers several advantages over other excitation sources, such as flame atomic absorption spectroscopy and graphite furnace atomic absorption spectroscopy. Firstly, the high temperature of the plasma ensures complete atomization and ionization of the sample, leading to higher sensitivity and lower detection limits. Secondly, the plasma provides a stable and robust excitation source, resulting in reliable and reproducible analytical results. Additionally, the high energy density of the plasma allows for the analysis of refractory elements that are difficult to atomize using other excitation sources.ICP-ES is a versatile technique that can be used for the analysis of a wide range of samples, including liquids, solids, and gases. It is commonly used for the analysis of environmental samples, such as water, soil, and air, to monitor the levels of toxic elements and pollutants. In the pharmaceutical industry, ICP-ESis used for the analysis of drug formulations to ensure compliance with regulatory standards. In material science, ICP-ES is employed for the analysis of metals, alloys, and ceramics to determine their elemental composition and purity.In conclusion, Inductively Coupled Plasma Emission Spectroscopy (ICP-ES) is a powerful analytical technique that offers high sensitivity, wide dynamic range, and excellent precision for the analysis of trace elements in various samples. Its use of inductively coupled plasma as the excitation source provides several advantages over other excitation sources, making it a popular choice in analytical laboratories worldwide. With its versatility and reliability, ICP-ES is a valuable tool for research, quality control, and environmental monitoring applications.。

Mid-Infrared Spectroscopic Properties of Ultra-Luminous Infrared Quasars

Mid-Infrared Spectroscopic Properties of Ultra-Luminous Infrared Quasars

a r X i v :0807.3653v 1 [a s t r o -p h ] 23 J u l 2008Mon.Not.R.Astron.Soc.000,000–000(0000)Printed 23July 2008(MN L A T E X style file v2.2)Mid-Infrared Spectroscopic Properties of Ultra-LuminousInfrared QuasarsChen Cao,1,2,3,4⋆X.Y.Xia,5†Hong Wu,3S.Mao,6,5C.N.Hao,7,5Z.G.Deng 4,5,31School of Space Science and Physics,Shandong University at Weihai,Weihai,Shandong 264209,China2VisitingScholar,Harvard-Smithsonian Center for Astrophysics,Cambridge,MA 021383National Astronomical Observatories,Chinese Academy of Sciences,Beijing 100012,China 4Graduate University,Chinese Academy of Sciences,Beijing 100039,China5Tianjin Astrophysics Center,Tianjin Normal University,Tianjin 300384,China6Jodrell Bank Centre for Astrophysics,Alan Turing Building,University of Manchester,Manchester M139PL,UK 7Institute of Astronomy,University of Cambridge,Madingley Road,Cambridge CB30HA,UK23July 2008ABSTRACTWe analyse mid-infrared (MIR)spectroscopic properties for 19ultra-luminous infraredquasars (IR QSOs)in the local universe based on the spectra from the Infrared Spec-trograph on board the Spitzer Space Telescope .The MIR properties of IR QSOs are compared with those of optically-selected Palomar-Green QSOs (PG QSOs)and ultra-luminous infrared galaxies (ULIRGs).The average MIR spectral features from ∼5to 30µm,including the spectral slopes,6.2µm PAH emission strengths and [NeII]12.81µm luminosities of IR QSOs,differ from those of PG QSOs.In contrast,IR QSOs and ULIRGs have comparable PAH and [NeII]luminosities.These results are consistent with IR QSOs being at a transitional stage from ULIRGs to classical QSOs.We also find that the colour index α(30,15)is a good indicator of the relative contribution of starbursts to AGNs for all QSOs.Correlations between the [NeII]12.81µm and PAH 6.2µm luminosities and those between the [NeII],PAH with 60µm luminosities for ULIRGs and IR QSOs indicate that both [NeII]and PAH luminosities are approxi-mate star formation rate indicators for IR QSOs and starburst-dominated galaxies;the scatters are,however,quite large (∼0.7to 0.8dex).Finally the correlation between the EW (PAH 6.2µm)and outflow velocities suggests that star formation activities are suppressed by feedback from AGNs and/or supernovae.Key words:galaxies:active –galaxies:evolution –galaxies:interactions –quasars:general —infrared:galaxies1INTRODUCTIONSince the discovery of ultra-luminous infrared galaxies (ULIRGs,L IR >1012L ⊙)by the Infrared Astronomical Satel-lite (IRAS )in the 1980’s (e.g.,Houck et al.1985),it is widely accepted that ULIRGs result from strong interac-tions/mergers between gas-rich disk galaxies.These mergers form elliptical galaxies and ULIRGs are an important inter-mediate stage in the process during which at least a frac-tion of ULIRGs manifest as dust-enshrouded QSOs (see,e.g.,Sanders &Mirabel 1996;Lonsdale et al.2006).In addition,active galactic nuclei (AGNs)triggered by mergers tend to appear at the final merging stages (e.g.,Sanders et al.1988,⋆E-mail:ccao00@ †E-mail:xyxia@ Zheng et al.1999,Cui et al.2001,Veilleux et al.2002and reference therein).There is mounting evidence that QSOs with far-infrared (FIR)excess have massive starbursts in their host galax-ies.For example,Canalizo &Stockton (2001)investigated 9QSOs with FIR excess and found that their host galaxies are tidally interacting or major merger systems with obvi-ous recent star-forming activities.From the detections of mid-infrared (MIR)/FIR 1emissions for FeLoBALs (Broad Absorption Line QSOs with low-ionisation and iron absorp-tion lines)by Multiband Imaging Photometer on Spitzer (MIPS;Rieke et al.2004)on board the Spitzer Space Tele-scope (Werner et al.2004),Farrah et al.(2007a)find that all1In this paper,MIR refers to 5-35µm,FIR 35-350µm,and IR 8-1000µm.2 C.Cao et al.their9FeLoBALs are extremely infrared(IR)bright,and concluded that these QSOs are in transition from ULIRGs to classical QSOs with ongoing or recent starbursts,because the iron absorption may be from iron ejected during star-bursts.Hao et al.(2005a)studied31QSOs/Seyfert’1s se-lected from the local ULIRG samples(termed as IR QSOs for simplicity).By comparing the FIR spectral index of IR QSOs with those of optically selected Palomar-Green QSOs(PG QSOs;Schmidt&Green1983),they argued that the FIR excess of IR QSOs relative to PG QSOs is from mas-sive starbursts and inferred star formation rate(SFR)in the host galaxies of IR QSOs.Recently,from studies of z∼6 QSOs with strong sub-mm emissions,Carilli et al.(2007), Wang et al.(2007)and Wang et al.(2008)concluded that massive starbursts also exist in their host galaxies.The con-clusion is consistent with the results of Hao et al.(2008)that high redshift(sub)mm-loud QSOs follow the same trend for FIR to bolometric luminosities established by low redshift IR QSOs(Hao et al.2005a).All these studies suggest that there exists a transitional stage,during which both the cen-tral black hole and the spheroidal component of QSO hosts grow rapidly in a coeval fashion.However,there is still a debate about the origin of FIR emission from QSOs,because one cannotfirmly exclude the possibility that FIR emissions are from dust tori heated by central AGNs(for detailed discussions see Haas et al.2003). Moreover,from the molecular gas properties of PG QSOs with IR excess and comparisons with ULIRGs,Evans et al. (2006)find that the L IR/L′CO and L IR/L′HCN ratios for PG QSOs are higher than those of ULIRGs,implying that AGNs contribute significantly to the dust heating and hence to the FIR emission.Therefore,other SFR indicators besides the FIR emission for QSOs are important for further under-standing the coeval growth of supermassive black holes and their host galaxies.Recently,the QUEST(Quasar and ULIRG Evolution Study)group(see Schweitzer et al.2006;Netzer et al.2007) reported the detection of polycyclic aromatic hydrocarbon (PAH)emission features in PG QSOs using the Infrared Spectrograph(IRS)on Spitzer(Houck et al.2004).For11 out of26PG QSOs PAHs have been clearly detected. Furthermore,the average spectrum of the undetected15 PG QSOs also shows PAH features.Since the PAH emis-sions are closely related to star formation,not to AGNs(see, e.g.,Shi et al.2007),such detections strongly suggest that star formation occurs widely in QSOs.Their analysis shows that30%or more FIR emission in these PG QSOs is from starbursts.Furthermore,given that the low-excitationfine-structure emission line[NeII]12.81µm is one of the dominant emission lines of HII regions and that the PAH molecules are easily destroyed by high energy photons from AGNs(e.g., Wu et al.2007),[NeII]emission may be an alternative,per-haps even better,tracer of star formation for QSOs(see§4).In this paper we study the MIR spectroscopic properties of IR QSOs based on Spitzer IRS observations,and examine their connections and evolutionary relations to ULIRGs and PG QSOs.The sample selection,data acquisition and reduc-tion are described in Sect.2and3.The major results and discussions are given in Sect.4and5.Finally we summarise our results in Sect.6.We adopt cosmological parameters H0=70km s−1Mpc−1,Ωm=0.3,andΩΛ=0.7throughout this paper.2SAMPLE SELECTIONIR QSOs are defined as type1AGN with L IR(8−1000µm)>1012L⊙(Zheng et al.2002).Our basic IR QSO samples are compiled from ULIRG samples with spectro-scopic information,plus the IR QSOs obtained directly from the cross-correlation of the IRAS Point-Source cata-log with the ROSAT All-Sky Survey Catalog.The ULIRG samples consist of118ULIRGs from1Jy ULIRGs survey (Kim&Sanders1998)and97ULIRGs from the QDOT redshift survey(Lawrence et al.1999).The total number of IR QSOs is31,about one third of all the IR QSOs found in a complete redshift survey with15,411IRAS galax-ies and about900ULIRGs(PSCz;Saunders et al.2000). Thus it should be a representative sample of IR QSOs(see Zheng et al.2002and Hao et al.2005a for more detailed de-scriptions).We searched the Spitzer archival data and found that18 out of31IR QSOs have been observed by IRS and the data are available(see Table1).Notice that9of the10IR QSOs (out of a total of118ULIRGs)from the1Jy ULIRG sam-ple are included in our sample.The other9IR QSOs are from QDOT(4)and other QSO samples.In addition we include the object IRAS F14026+4341,which is classified as a hyper-luminous infrared galaxy(with L FIR>1013L⊙, Rowan-Robinson2000)and a broad absorption line quasar (Low et al.1989).Our sample includes90%(50%)IR QSOs out of1Jy(QDOT)ULIRGs,and thus should be an overall representative sample.We checked that our results are es-sentially unchanged if we focus only on the9IR QSOs from the1Jy ULIRG samples,and thus our compiled sample has no significant biases.14of the19objects have both low-and high-resolution IRS observations,while four(3C48,IRAS F02054+0835, PG1543+489,and IRAS F20036−1547)have only low-resolution observations,and one(IRAS F21219−1757)has no Long-Low(LL)mode(see§3.1)observation(see Table3).The IRS low-resolution spectra of a sample of Palomar-Green QSOs(PG QSOs)are retrieved from Spitzer GTO and GO archival data(programs14,3187,3421,and20142; see Table4).We remove objects whose MIR spectra have a low S/N ratio or redshift larger than0.27to guarantee reliable measurements of rest-frame30µmfluxes.The num-ber of PG QSOs is19(see Table1),the same as the num-ber of IR QSOs.This PG QSO sample is mainly used for studying the MIR spectral slopes benefiting from the full wavelength coverage from∼5-30µm of their low-resolution spectra.We also collected another PG QSO sample with 22objects studied by Schweitzer et al.(2006)(after exclud-ing four objects that have been classified as IR QSOs and grouped into IR QSO sample).This second PG QSO sam-ple has deep SL mode observations(5.2-14.5µm)and is thus suitable for studying weak PAH emission features in continuum-dominated QSOs,and for analysing properties of the MIRfine-structure lines(especially[NeII]12.81µm line in this work)from high-resolution observations.The sample of Ultra-Luminous Infrared Galaxies (ULIRGs)is selected based on the IRAS1Jy sample of ULIRGs(Kim&Sanders1998),which have optical spectro-scopic observations by Veilleux et al.(1999)and Wu et al. (1998).Their IRS low-resolution spectra are retrieved from Spitzer GTO archival data(program ID105;see Table4).Mid-IR Spectral Properties of IR QSOs3The number in our ULIRG sample is35(see Table2), including all spectral types except type1AGNs,namely Seyfert2’s,LINERs,and HIIs as classified by Veilleux et al. (1999)and Wu et al.(1998)from diagnostic diagrams of op-tical lines.3DATA ACQUISITION AND REDUCTION3.1Mid-infrared spectra from Spitzer IRSThe MIR spectra are acquired from the Spitzer archival data using the Leopard software(see Table3for the integrated exposure times and program IDs for IR QSOs).The data (versions13.2to15.3of Spitzer pipeline reduction)include low-resolution(Short-Low[SL]&Long-Low[LL]modes, R∼60-120and wavelength range:5.2-38.0µm)IRS spectra for IR QSOs,PG QSOs,and ULIRGs,and high-resolution (Short-High[SH]&Long-High[LH]modes,R∼600and wavelength range:9.9-37.2µm)IRS spectra for most of the IR QSOs.We use the SMART software(Higdon et al.2004) for data reduction,including the removal of rogue pixels, sky subtraction,and spectral extraction and analysis.The sky backgrounds for low-resolution(SL&LL modes)spectra are subtracted by differencing the adjacent sub-slit positions (1st&2nd nods).No background subtraction is performed for the high-resolution(SH&LH modes)spectra,but this does not affect MIRfine-structure line measurements(see, e.g.,Farrah et al.2007b).The slit widths of3′′.6to11′′.1 include most of the emission from the QSO and its host galaxy,so no aperture corrections are performed.For the low-resolution spectra we use the12µm and25µmflux densi-ties from IRAS(or ISO if the IRASfluxes are upper limits or not available)to scale the spectra by multiplying by a small factor,which is more suitable for the comparison between MIR and FIR properties in our statistics.The scaling factors for IR QSOs and ULIRGs are often close to unity,typically less than1.12.However,for PG QSOs the scaling factors are larger,typically∼1.5.This may be caused by variabilities of quasars in MIR(e.g.,Neugebauer&Matthews1999)and/or contamination of companions(environments)for IRAS(or ISO)measurements.3.2Measurements of PAH and mid-infraredfine-structure linesThefluxes of PAH emission at6.2µm are measured by inte-grating theflux above a local continuum from6.0-6.5µm ap-proximated by a second-order polynomial(e.g.,Spoon et al. 2007;Desai et al.2007).The uncertainties(1σ)in the mea-surements are20%on average(varying from∼5%for PAH strong objects to about50%for those with only marginally detectable PAH features).The equivalent widths(EW)of 6.2µm PAH feature are obtained from dividing the inte-grated PAHflux by the continuumflux density below the peak of the feature.Upper limits(3σ)are given by adopt-ing typical widths of∼0.2µm for the6.2µm PAH feature 2One exception is3C273for which no scaling was per-formed since it exhibits large variabilities in the MIR (Neugebauer&Matthews1999;Hao et al.2005b).(Smith et al.2007),which is similar to the value,∼0.6µm, used for the7.7µm feature by Schweitzer et al.(2006).Note that we do notfit Gaussian or Lorentzian pro-files to measure PAH emissions(e.g.,Schweitzer et al.2006; Imanishi et al.2007)due to the relative weakness of PAH features in QSOs compared to their strong dust continuum. Since the three IR QSOs in the QUEST sample(I Zw1, Mrk1014,PG1613+518)have7.7/6.2flux ratios of4.2, 4.6, 4.9,respectively,similar to that of NGC6240(4.7, Armus et al.2006),we estimate the6.2µm PAHfluxes for PG QSOs in the Schweitzer et al.(2006)sample(which has only7.7µm measurements)by taking a7.7/6.2flux ratio of 4.7.Thefluxes of the ionised neonfine-structure lines in the MIR([NeII],[NeV],[NeIII]at12.81,14.32,and15.56µm) for IR QSOs are measured based on the high-resolution IRS spectra,using the IDEA spectral analysis tool of the SMART software.Thefluxes are measured byfitting a single Gaussian superposed on a local continuum approx-imated by a second-order polynomial.Flux upper limits (3σ)are derived adopting typical line widths of600km s−1 (Schweitzer et al.2006).Thefluxes of the[NeII]12.81µm line for some ULIRGs in our sample are from Farrah et al. (2007b).4RESULTS4.1Mid-infrared spectral characteristics ofIR QSOsFig.1shows the low-resolution Spitzer IRS MIR spectra of 18IR QSOs in our sample(except IRAS F21219−1757which has no LL mode observation).The dotted and dashed lines show the PAH features and the MIRfine-structure neon,sul-phur,and oxygen lines.The shaded bar denotes the silicate emission/absorption feature centred at9.7µm.One can see from these spectra that the PAH features at6.2,7.7,8.6,11.2 &12.7µm and the MIRfine-structure emission lines,such as[NeII]12.81µm,[NeV]14.32µm,[NeIII]15.56µm,[NeV] 24.32µm&[OIV]25.89µm are present in most IR QSOs,al-though some emissions are weak for most of them.One can also see the silicate absorption feature at9.7µm for several IR QSOs(F00275,F13218,Mrk231,F11119and F15462), which are rarely seen in PG QSOs.For comparison,we show the average MIR spectra of IR QSOs,PG QSOs and ULIRGs in Fig.2.The average spectra of ULIRGs and PG QSOs are from Hao et al.(2007).It is clear from Fig.2that the slope of MIR continua from15µm to30µm of IR QSOs is intermediate between that of ULIRGs and QSOs.We also show in the samefigure the spectra of two representative IR QSOs(PG1613+658and Mrk231).Their MIR spectra are intermediate between the average spectra of ULIRGs and PG QSOs.However,from the infrared,optical and X-ray observations,PG1613+658has the characteristics of classical QSOs(Zheng et al.2002),while Mrk231is an on-going merger with high SFR from its hundred-pc scale molecular disk(Downes&Solomon1998).In order to clarify more quantitatively the differences in the spectral properties,we study the properties of6.2µm PAH,[NeII]12.81µm luminosities,and MIR colour indices α(30,15),for IR QSOs,PG QSOs,and ULIRGs.The in-frared colour index is defined as4 C.Cao et al.Figure1.Low-resolution Spitzer/IRS mid-infrared spectra of18IR QSOs in our sample(except for IRAS F21219−1757which has no LL mode observation),all have been de-redshifted and shifted upward for clarity.The dotted lines show the PAH features at6.2,7.7, 8.6,11.2&12.7µm,and the dashed lines show the mid-infraredfine-structure lines of[SIV]10.51µm,[NeII]12.81µm,[NeV]14.32µm, [NeIII]15.56µm,[SIII]18.71µm,[NeV]24.32µm&[OIV]25.89µm.The shaded bar denotes the silicate emission/absorption feature centred at9.7µm.Mid-IR Spectral Properties of IR QSOs5Figure1.Continued.log(Fν(λ2)/Fν(λ1))α(λ1,λ2)=−6 C.Cao etal.Figure2.Average MIR IRS low-resolution spectra of IR QSOs(red solid line)in our sample,and QSOs(green),ULIRGs(black) from Hao et al.(2007),normalised to f(15µm)=1(denoted by a black dot).Spectra of two representative IR QSOs(PG1613+658and Mrk231)are also shown by red dashed and red dot-dashed lines.nitude higher than those of PG QSOs.Therefore,the dif-ferent properties of6.2µm PAH and[NeII]12.81µemissionsbetween IR QSOs and PG QSOs are unlikely from differentdust tori,instead the differences arise because of differentstar formation properties(see section4.2).In summary,the mid-IR spectroscopic properties,in-cluding the continuum slope and emission line strengths,of IR QSOs,PG QSOs and ULIRGs are consistent withthat IR QSOs are in a transitional phase from ULIRGs toclassical QSOs,confirming the results from previous studies(Canalizo&Stockton2001;Hao et al.2005a).4.2Statistics on spectral parametersIn this subsection,we will use the MIR spectroscopic fea-tures,including the MIR continuum slopeα(30,15),6.2µmPAH andfine-structure emission lines,to investigate the ori-gin of MIR emissions of IR QSOs.We will also use theseproperties,combined with FIR and optical properties,todisentangle the starburst and AGN contributions in theseobjects.Fig.5shows the relations ofα(30,15)vs.α(60,25)(left panel),α(30,15)vs.the FIR excess L60µm/L bol(mid-dle panel),andα(30,15)vs.the EW(PAH6.2µm)(rightpanel)for both IR QSOs and PG QSOs.It is clear fromFig.5that the colour indices ofα(30,15)andα(60,25)are closely correlated,indicating thatα(30,15)can expressthe relative strength of FIR to MIR emission for QSOs.Themiddle and right panels of Fig.5show the correlations be-tweenα(30,15)with FIR excess L60µm/L bol,and betweenα(30,15)with the EW(PAH6.2µm).The EW(PAH6.2µm)is the ratio of PAH6.2µm emission line to∼6µm contin-uum.Since the6.2µm PAH emission is from star forma-tion regions,and the6µm continuum traces the AGN con-tribution(e.g.,Gallagher et al.2007),thus the EW(PAH6.2µm)expresses the relative contribution of star forma-tion to AGN(Schweitzer et al.2006;Armus et al.2007).Infact,Desai et al.(2007)also found the strong correlation be-tween infrared spectral slope and the EW(PAH6.2µm)forULIRGs,especially for ULIRGs with Seyfert1and Seyfert2optical spectra,while our results extend such relation to in-frared luminous QSOs and PAH detected PG QSOs.Weconclude thatα(30,15),FIR excess and EW(PAH6.2µm)Mid-IR Spectral Properties of IR QSOs7Figure 3.From left to right:histograms of MIR colour index α(30,15),the EW (PAH 6.2µm),the 6.2µm PAH and 12.8µm [NeII]line luminosities for ULIRGs (top),IR QSOs (middle),and PG QSOs (bottom)with detectable PAH and [NeII]emissions.The mean values are labelled in the panels,and the number of objects used in each histogram is in the bracket.Notice that the histogram of L([NeII])for PG QSOs is from the sample by Schweitzer et al.(2006),while the [NeII]luminosities for ULIRGs are derived from Farrah et al.(2007b).can serve as indicators of the relative contributions of star-bursts to AGNs (Hao et al.2005a and see below).Ho &Keto (2007)suggest that the ionised neon fine-structure lines [NeII]12.81µm and [NeIII]15.56µm can be used as a SFR indicator for star-forming galaxies.Farrah et al.(2007b)extend this relation to ULIRGs.In addition,Schweitzer et al.(2006)found a strong correla-tion between the far-infrared continuum (L 60µm )and low-ionisation [NeII]line emission for both PG QSOs and ULIRGs,and argued that the [NeII]line can also be used to estimate the SFR in QSO host galaxies.One advantage to use [NeII]12.81µm as a SFR estimator is that it suf-fers much less extinction than optical lines,such as Hαand [OII]3727˚A .However,there is still a debate about the ori-gin of [NeII]emission,because the narrow line region of QSOs may also contribute substantially (e.g.,Ho &Keto 2007).Therefore,it is worth investigating the origin of [NeII]12.81µm emission for IR QSOs by comparing the multi-wavelength properties of PG QSOs,IR QSOs and ULIRGs.Fig.6shows the relation between PAH 6.2µm and [NeII]12.81µm luminosities for IR QSOs,ULIRGs and PG QSOs with firmly detected [NeII]12.81µm and PAH 6.2µm emissions.Because the PAH emissions are purely from star formation regions (Shi et al.2007),the tight corre-lation between 6.2µm PAH and [NeII]12.81µm luminosities (at a statistical level of >99.99%with the Spearman Rank-order test)demonstrates that (at least part of)the [NeII]12.81µm emission is also from star formation regions.Note that for the PG QSOs shown in Fig.6,their [NeII]12.81µm luminosities normalised by the bolometric luminosities of AGNs (L [NeII]/L bol ratios,see below)are about three times higher than that of PAH undetected PG QSOs (see also Schweitzer et al.2006).Therefore,it is likely that the star formation contributes significantly to the [NeII]emission not only for IR QSOs,but also for PG QSOs with detectable PAH emissions (Netzer et al.2007).The mean values of L [NeII]12.81µm /L bol ratios are 3.4±3.5×10−4,5.3±3.6×10−5,8.1±5.2×10−5and 2.7±1.4×10−5for IR QSOs,PG QSOs,PAH-detected and PAH-undetected8 C.Cao etal.Figure4.Histograms of the6.2µm PAH(left)and12.8µm[NeII]line(right)luminosities normalised by the bolometric luminosity for IR QSOs(top)and PG QSOs(bottom)with detectable PAH and[NeII]emissions.The mean values are labelled in the panels,and the number of objects used in each histogram is in the bracket.Notice that the histogram of L[NeII]/L bol for PG QSOs is from the sample of Schweitzer et al.(2006).PG QSOs,respectively.Thus for the same bolometric lumi-nosity of a central AGN,the mean[NeII]12.81µm luminosityof IR QSOs is about one order of magnitude higher than thatof classical QSOs.Taken together with the tight correlationbetween[NeII]and PAH luminosities(see above),we con-clude that the[NeII]12.81µm emission of IR QSOs is mainlyfrom star formation,while the contribution from the narrowline region of AGNs is not significant( 10%).5DISCUSSIONBy comparing the MIR spectroscopic properties of IR QSOs,ULIRGs and PG QSOs,we found that the indicators of rel-ative contributions of starbursts to AGNs(such as colourindexα(30,15)and EW[PAH6.2µm])for IR QSOs are be-tween those of ULIRGs and PG QSOs.These results are con-sistent with thefindings of Canalizo&Stockton(2001)andHao et al.(2005a)that(at least some)infrared luminousQSOs(IR QSOs)are at a transitional stage from ULIRGs toclassical QSOs.Below we consider the star formation ratesand AGN/star formation feedback in more detail.5.1SFR determined by the[NeII]12.81µm andPAH luminositiesAs we argued,the AGN contribution to[NeII]emission forULIRGs and IR QSOs is probably very small,and there isa tight correlation between[NeII]12.81µm and PAH6.2µmluminosities(see§4.2).We examine in more detail how theycan be used as approximate SFR indicators for ULIRGs andIR QSOs.Fig.7shows the[NeII]12.81µm(top panel)and PAH6.2µm(bottom panel)luminosities versus the60µm lumi-nosity for IR QSOs,PG QSOs and ULIRGs withfirmly de-tected[NeII]12.81µm and PAH6.2µm emissions.A Spear-man Rank-order analysis show that both correlations aresignificant at>99.99%level.The dashed lines in Fig.7represent the least-squares regressionfits:log L NeII=(0.90±0.06)log L60µm−(2.22±0.69),(2)Mid-IR Spectral Properties of IR QSOs9Figure5.From left to right:MIR colour indexα(30,15)vs.infrared colour indexα(60,25);vs.infrared excess(L60µm/L bol);vs. EW(PAH6.2µm)for IR QSOs(redfilled circle)and PG QSOs(green and black open circle for PG QSOs from our sample and Schweitzer et al.2006).The horizontal and/or vertical bars on the bottom right of each panel indicate the mean errors on theα(60,25), L60µm/L bol,andα(30,15)values.log L PAH=(0.78±0.06)log L60µm−(0.31±0.74).(3) Thefitting formula(2)is consistent with that of Ho&Keto (2007)for star-forming galaxies(within the large errors). Note that our sample objects have much higher60µm and [NeII]luminosities than their star-forming galaxies.Thus both[NeII]12.81µm and PAH6.2µm luminosities can be used as approximate SFR indicators not only for normal star-forming galaxies,but also for galaxies with high in-frared luminosities,such as ULIRGs and IR QSOs(see Brandl et al.2006and Farrah et al.2007b).However,the mean scatters(about0.7to0.8dex)in the relation of[NeII]12.81µm,PAH6.2µm with60µm lumi-nosities are larger than that(about0.6dex)of star-forming galaxies with lower infrared luminosity(Ho&Keto2007). Comparing Fig.7with Fig.6,one can see that the scatter in the relation of[NeII]12.81µm vs.PAH6.2µm luminosi-ties(about0.6dex)is smaller than that in the relations of [NeII]12.81µm,PAH6.2µm luminosities with L(60µm).It is also clear that most large scatters are from ULIRGs.This is perhaps not surprising since the range in the9.7µm sil-icate absorption depth among ULIRGs is quite large(see Armus et al.2007,Spoon et al.2007).In short,the large scatters for ULIRGs seen in the relations may be due to complicated,patchy extinctions among these galaxies in the MIR band.A detailed discussion on extinction for ULIRGs can be found in Farrah et al.(2007b).5.2AGN/Star formation feedback in thetransitional stageOne explanation for the observed correlation between spheroidal and black hole mass(e.g.,Magorrian et al. 1998;Ferrarese&Ford2005)is that star formation and the growth of central black holes may be self-regulated: AGNs/star formation can drive nuclear outflows which in turn suppress further cooling and star formation (Silk&Rees1998).While the detailed processes are still to be understood,it is now increasingly clear that feedback and outflows play an important role in galaxy formation and evolution.So far most observational evidence for AGN feedback is from radio observations at the centre of clusters or groups of galaxies(Batcheldor et al.2007).On the galaxy scale, evidence is still limited.As discussed above,IR QSOs have high SFRs and accretion rates(Hao et al.2005a),outflow properties in these objects may thus provide hints on the feedback processes on galaxy or group scale.It is well known that low-ionisation broad absorption line QSOs(loBAL QSOs)comprise about15%of BAL QSO population.They are defined as a subclass of BAL QSOs with an obvious blueshifted absorption in Mg IIλλ2795,2802 and other low-ionisation species(Weymann et al.1991). Such absorption troughs arise from resonance absorption by outflowing gas and dust(Voit et al.1993).In addition,there is a rare class of loBAL QSOs,showing absorption features from excited iron(termed as FeLoBAL QSOs).The outflow velocities for most BAL QSOs span a large range,up to a few times104km s−1,which may be formed on a scale of<1pc and directly associated with the wind from an accretion disk or molecular torus(Weymann et al.1985). However,recent spectral analyses based on Keck observa-tions for LoBAL QSOs or FeLoBAL QSOs reveal that the outflow velocities of some LoBAL QSOs range from several hundred to several thousand km s−1and the wind is from regions of a few hundred pc(e.g.,Ganguly&Brotherton 2008;Hamann et al.2000;de Kool et al.2002),which is much larger than the central engine of AGNs,but simi-lar to the size of the nuclear starburst region of ULIRGs (Downes&Solomon1998).On the other hand,Canalizo&Stockton(2002)stud-ied four loBAL QSOs at z<0.4(Mrk231,IRAS14026+4341, IRAS F07599+6508,PG1700+518;all four are in our sam-ple)and found that all are ULIRGs with merging signa-tures.They argued that loBAL QSOs cannot simply be ex-plained by orientation effects,rather,they are directly re-。

电感耦合等离子体原子发射光谱法测定工业氢氧化铍中杂质元素

电感耦合等离子体原子发射光谱法测定工业氢氧化铍中杂质元素

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3M Emphaze AEX Hybrid Purifier 数据表说明书

3M Emphaze AEX Hybrid Purifier 数据表说明书

Data Sheet 3M™ Emphaze™ AEXHybrid PurifierThe 3M™ Emphaze™ AEX Hybrid Purifier is improving biopharmaceutical manufacturing processes, including recombinant protein and especially monoclonal antibody (mAB).The 3M Emphaze AEX Hybrid Purifier is a synthetic, multi-mechanism single-use purifier used for biopharmaceutical clarification. It delivers consistent, high-purity clarified process fluid by reducing negatively charged DNA, HCP, endotoxin, and cell debris through a combination of chromatographic and size exclusion mechanisms.PerformanceIn a representative monoclonal antibody (mAb) manufacturing process, when used in combination at the clarification stage with Zeta Plus™ depth filters and LifeASSURE™ membrane filters, the Emphaze AEX Hybrid Purifier increases process efficiency and protein purity post Protein A.Customers experience benefits in typical monoclonal antibody purification processes using the Emphaze AEX Hybrid Purifier.•Nominal 20-40% HCP and greater than 4 log DNA reduction •Consistent output turbidity(<5NTU)•Increase product purity post-protein A •Downsizing of the sterilizing grade membrane •Reduce turbidity post viral inactivation/ neutralization step•Reduce impurities load on downstream AEX column Sterilization/SanitizationEmphaze AEX Hybrid Purifier products, listed in Table 1, can be sterilized or sanitized. Refer to Table 1 on page 3 for more information.As we worked with customers to qualify the Emphaze AEX Hybrid Purifier, we heard that sterilization/ sanitization compatibility is an important feature.We announced that 3M Emphaze AEX Hybrid Purifier, with part and model numbers that end in an R, canbe sterilized/sanitized across various aqueous-based biopharmaceutical processes, including vaccine purification. See Table 1 for the product namesand numbers.Note: Only these products can be sterilized and sanitized.Layer 3Layer 4Zone 4-6100μmProduct Selection/Specification(NOTE:R after product and model name indicates the sterilization/sanitization compatible products)A full support package is available for the 3M™ Emphaze™ AEX Hybrid Purifier. This package includes Installation and Operation Instructions, Certificate of Quality or Certificate of Lot Conformance, and a Regulatory Support File.1.Capsule Fill Volume is defined as the volume of liquid that is required to fill the capsule.2.Post Blow-Down Hold-Up Volume is defined as the volume of the residual liquid after air/gas blow down.3.Do not use this product for continuous service with compressed gasses. The use of compressed gas is permissible for integrity testing and blowdown purposes.4.A Preconditioning Flush is required for the product to be compliant with USP Biological Reactivity Tests, including USP <87> and <88> Class VI.The flush solution can be a buffer or 25–150mM sodium chloride solution. Refer to Installation and Operation Instructions for complete instructions on how to perform the preconditioning flush.Compliance•U SP <87> Biological Reactivity Tests, In Vitro: All wetted component materials of the 3M™ Emphaze™ AEX Hybrid Purifier products were tested and met the requirements of USP <87> Biological Reactivity Tests, In Vitro. The media was subjected to the required preconditioning flush prior to testing.•U SP <88> VI Biological Reactivity Tests, In Vivo: All wetted component materials of the 3M™ Emphaze™ AEX Hybrid Purifier products were tested and met the requirements of USP <88> Biological Reactivity Tests, In Vivo. The media was subjected to the required preconditioning flush prior to testing.Animal-Derived Material Statement:In order to assess the BSE/TSE risk associated with the 3M Emphaze AEX Hybrid Purifier products, we have contacted our suppliers of raw materials and performed an evaluation of our production processes to determineif any of the materials used are of animal origin. The result of our survey and inquiries of our raw material suppliers has revealed thatthe polypropylene resins used in the nonwovens and the glass-filled polyphenylene oxide/polystyrene resin used in molded parts may contain tallow. Our suppliers have indicated that these parts that use tallow derivatives are processed at conditions conforming to the requirements of the European Medicines Agency note for guidance EMEA/410/01 rev.3.Intended Use: Single-use processing of aqueous based biological pharmaceuticals (drugs) and vaccines strictly following the product operating instructions and cGMP requirements, where applicable.Prohibited Use: As a component in a medical device that is regulated by any agency, and/or globally exemplary agencies, including but not limited to: a) FDA, b) European Medical Device Directive (MDD), c) Japan Pharmaceuticals and Medical Devices Agency (PMDA); Applications involving permanent implantation into the body; Life-sustaining medical applications; Applications requiring food contact compliance.Product Selection and Use: Many factors beyond 3M’s control and uniquely within user’s knowledge and control can affect the useand performance of a 3M product in a particular application. As a result, end-user is solely responsible for evaluating the product and determining whether it is appropriate and suitable for end-user’s application, including completing a risk assessment that considers the product leachable characteristics and its impact on drug safety conducting a workplace hazard assessment and reviewing all applicable regulations and standards (e.g., OSHA, ANSI, etc.). Failure to properly evaluate, select, and use a 3M product and appropriate safety products, or to meet all applicable safety regulations, may result in injury, sickness, death, and/or harm to property.Warranty, Limited Remedy, and Disclaimer: Unless a different warranty is specifically stated on the applicable 3M product packaging or product literature (in which case such warranty governs), 3M warrants that each 3M product meets the applicable 3M product specification at the time 3M ships the product. 3M MAKES NO OTHER WARRANTIES OR CONDITIONS, EXPRESS OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, ANY IMPLIED WARRANTY OR CONDITION OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR ARISING OUT OF A COURSE OF DEALING, CUSTOM, OR USAGE OF TRADE. If a 3M product doesnot conform to this warranty, then the sole and exclusive remedy is, at 3M’s option, replacement of the 3M product or refund of the purchase price.Limitation of Liability: Except for the limited remedy stated above, and except to the extent prohibited by law, 3M will not be liable for any loss or damage arising from or related to the 3M product, whether direct, indirect, special, incidental, or consequential (including,but not limited to,lost profits or business opportunity), regardless of the legal or equitable theory asserted, including, but not limited to, warranty, contract, negligence, or strict liability.3M Purification Inc.3M, Emphaze, LifeASSURE and Zeta Plus are3M Separation and Purification Sciences Division trademarks of 3M Company. All other trademarks 400 Research Parkway, Meriden, CT 06450 USA are property of their respective owners.Phone 1-203-237-5541Please recycle. Printed in USA © 3M 2019.Web /bioprocessing All rights reserved. 70201600056 REV 07/2019。

四苯乙烯衍生物英文翻译

四苯乙烯衍生物英文翻译

A wide variety of substituents has been attached to phenyl blades of tetra-phenylethylene moiety to enhance its electronic and optical prop-erties. Xu et al.[11] reported that triphenylethylene derivatives also show remarkable aggregation-induced emission properties. In this letter, we report The synthesis and their aggregation-induced emission properties of three new oligomers, in which triphenyleth-ylene units directly connected to tetraphenylethene moiety. These compounds exhibit remarkable aggregation-induced emission properties, which make them promising candidates as luminescent materials for electroluminescence applications.各种附加到四苯乙烯的苯环上的取代基是来提高其电子性质和光学。

并且徐等人报道了三苯乙烯衍生物也显示出显著的聚集诱导发光特性。

在这篇论文中,我们报告了三个新的低聚物的聚集诱导荧光特性和他们的合成,其中三苯乙烯单元直接连接到四苯乙烯部分。

这些化合物表现出显著的聚集诱导荧光特性,使他们在电致发光的应用上成为有前途的候选发光材料。

Our strategy for the synthesis of tetraphenylethylene based core units and triphenylethylene boronic acid derivatives is outlined in Scheme 1 . The 1-(4-bromophenyl)-1,2,2-triphenylethylene (1) [12 ]was synthesized via the reaction of 4-bromobenzophenone with diphenylmethyllithium at a lower temperature (0C) followed by acid catalysed dehydration of the resulting alcohol in 85% yield. The dimethoxy-substituted TPE 2 was obtained from 4,4-dimethoxybenzophenone and4-bromobenzophenone using conven-tional McMurry reaction in 40% yield. Low yield of 2 is due to the formation of dibromo-tetraphenylethylene and tetra-methoxy-tetraphenylethylene derivatives. Tetrakis(4-bromophenyl)ethyl-ene (3)13was prepared from 4,4 -dibromobenzophenone through a titanium(0)-catalysed McMurry reaction in 76% yield. Benzophe-none (4) was converted into1-bromo-4-(2,2-diphenylvinyl)ben-zene (6) 14 using diethyl 4-bromobenzylphosphonate in the presence of potassium tert -butoxide as a base in anhydrous THF at 0 C temperature. The related methoxy-substituted triphenyl-ethylene derivative7 was obtained in a similar manner starting from 4,4-dimethoxybenzophenone ( 5) in 82% yield.我们对四苯乙烯基础核心单元和三苯乙烯硼酸衍生物的合成策略在方案1概述。

SPECTRAL PROPERTIES

SPECTRAL PROPERTIES
oo
f(x) = ~ fke ik~ k=-~
be its Fourier series, wherei,j = Nhomakorabea...n,
where the numbers fk are cosine-Fourier coefficients of a real even function (see below); then, if v0...v~ are the cosine-Fourier coefficients of the trigonometric polynomial v(x), the entries of the vector
of Toeplitz-plus-Hankel matrices
89
Some results in this paper are in a preliminary form; indeed, the author is aware that a wider class (in particular, some unbounded sequence) of T + H matrices can be considered, by means of the approach described in [22, 23]. This generalization, as well as the treatment of block structured matrices, will be the subject of further work. 2. G e n e r a t i n g functio.ns o f s t r u c t u r e d m a t r i c e s Let f(x) E LCC[-~r, rr] be a real function and let

C. Peptide Mass Fingerprinting

C. Peptide Mass Fingerprinting

SECTION III INFORMATICSPROTEOMIC INFORMATICSSteven A.Russell,*William Old,{Katheryn A.Resing,{and Lawrence Hunter**Center for Computational Pharmacology,University of Colorado Health Sciences CenterAurora,Colorado80045{Department of Chemistry and Biochemistry,University of Colorado,Boulder,Colorado80309I.What is Proteomics?II.Proteomic InformaticsIII.Mass Spectrometry and Shotgun ProteomicsA.Brief Overview of Spectrometers and SpectrometryIV.Identifying ProteinsA.Database Methods for Peptide CharacterizationB.De Novo Peptide CharacterizationC.From Peptides to ProteinsV.Post-hoc Validation of Protein Identification Program OutputVI.QuantificationVII.Detection of Protein IsoformsA.Detection of Protein PhosphorylationVIII.Systems and Workflow IssuesA.Data Exchange,Sharing,and Privacy IssuesIX.ConclusionX.Appendix:List of Mentioned Algorithms by TopicA.Identification Algorithms for MS/MS Database CorrelationB.Filtering and Visualization ToolsC.Peptide Mass FingerprintingD.Sequence Tag IDE.De NovoF.Post-Translational Modification IdentificationG.OtherH.Analysis Systems(LIMS)I.Quantitative SoftwareReferencesI.What Is Proteomics?Proteomics is the study of the protein composition of a complex,an organelle, a cell,or even an entire organism.Proteomic characterizations provide crucially important information about the structure and function of cells and more complex biological systems.Although protein sequences and many regulatory signals are encoded in DNA,aspects of the protein composition of a cell,such as expression levels,splice variants,and post-translational modifications(e.g., cleavages or covalent chemical modifications),are not so encoded.IndeedINTERNATIONAL REVIEW OF129 NEUROBIOLOGY,VOL.61Copyright2004,Elsevier Inc.All rights reserved.0074-7742/04$35.00130RUSSELL et al.these aspects of protein compositionfluctuate rapidly and over an enormous range;suchfluctuations play a variety of critical roles in biological processes. Sensitive and accurate assays of relevant aspects of complex protein mixtures are needed to fully understand and influence these processes.Such assays are rapidly improving along a variety of dimensions,including the number of proteins that can be characterized simultaneously,the aspects of these proteins that can be assayed,and the cost of doing such characterizations.As proteomics technology continues to improve,it is likely that it will play an increasingly important role in basic research,medical applications,and the development of biotechnology.Spurred by the availability of whole genomic sequences,advances in mass spectrometry,and the development of relevant informatics techniques,proteo-mics research is growing rapidly from a trickle to aflood.Integration of this burgeoning class of information with other global surveys,such as metabolomics and mRNA profiling,helped spawn the newfield of systems biology(Kitano, 2002).1The promise of these tools includes the potential of development of per-sonalized and preventive medicine at the molecular level,as well as many other applications.However,before these methods can reach their potential,pro-teomics assays must become more informative,more reliable,and less dependent on extensive expertise of the operators.A set of ultimate goals for shotgun proteomic technology can be clearly stated.Ideally,proteomics should be able to do the following:Identify all of the proteins present in a complex mixture,such as whole cell lysates,ideally with su Y cient sensitivity tofind proteins present in single molecule per cell concentrations;Identify and characterize all of the isoforms of all proteins in a mixture, including splice variants,cleavage products,and post-translational covalent modifications;andQuantify the concentration of each protein and all of its isoforms.Of course the current state of the art is only partially able to fulfill these goals, but technical progress is rapid,and the existing technology already has had tremendous scientific impact.The informatics methods reviewed in this chapter play a key role in the ability to transform mass spectra data into information addressing the aforementioned goals.Although instruments or methods other than mass spectrometry(e.g.,antibody or aptamer arrays)show some potential for addressing these goals,because of their relative immaturity,the informatics methods relevant to them will not be reviewed.1Loosely speaking,systems biology is the characterization of biological systems by enumeration of a comprehensive list of components,their structure,and their dynamics.PROTEOMIC INFORMATICS131II.Proteomic InformaticsProteomics at present is afield that is in an exhilarating yet frustrating phase, with new ideas emerging almost daily,but with far less clarity regarding how the field will ultimately evolve.However,it is clear that this research will result in the production of thousands or even hundreds of thousands of mass spectra per day in many proteomics laboratories.Extracting the maximum scientific value from this activity depends crucially on informatics at many stages,from manag-ing the instruments to analyzing the resulting spectra.The purpose of this review is to describe the myriad roles that informatics plays in thefield,to identify some of the informatics challenges that remain to be resolved,and to suggest some future directions that proteomics informatics is likely to take.Our focus will be on informatics relevant to high-throughput,or‘‘shotgun,’’proteomics e V orts.III.Mass Spectrometry and Shotgun ProteomicsAlthough proteomic profiling wasfirst carried out in1975using two-dimensional polyacrylamic gel electrophoresis(2D-PAGE;also called two-dimensional gel elec-trophoresis or2DE)(O’Farrell,1975),the development of new mass spectrome-try(MS)methods has generated a renewed interest in such profiling.Early studies used blotting to membranes,followed by Edman sequencing to identify the proteins one amino acid at a time.An important methodological improvement involves a technique whereby protein‘‘spots’’2from2D gels are identified by excising the spots and carrying out in-gel proteolytic digests.The digest is an enzymatic fragmentation of the purified protein into a set of peptides.The peptides are recovered and analyzed by MS to obtain mass and sequence information,which can in turn be used to infer the protein in the original spot by matching the observed peptide masses and sequences to expected values calculated for each protein that could potentially have been present in the original sample(usually defined by a protein database derived from an organism’s genomic sequence).The recent increase in interest in proteomics was stimulated in1996when Matthias Mann’s laboratory published the application of this method to the analysis of proteins in yeast extracts resolved by2D-PAGE(Shevchenko et al., 1996).However,this method is limited by the di Y culties of2D gels(e.g.,limited dynamic range and the fact that basic and membrane proteins are poorly detected).Furthermore,2D-PAGE does not lend itself to high-throughput 2Isolated regions of a gel that putatively contain a single,purified protein isoform.132RUSSELL et al.analysis.These problems have led to the recent introduction of an approach that eliminates the use of gels altogether,sometimes referred to as shotgun proteomics (Yao et al.,2001).In this approach all of the proteins in a sample are simulta-neously digested with a protease,and then the large collection of peptides is analyzed to identify all of the proteins in the original sample.Typically the peptides are resolved into several fractions by ion exchange(IE)chromatography, and then the peptides in each IE fraction are analyzed by a reverse phase column directly coupled to a liquid chromatography mass spectrometer(LC-MS).This MudPIT(multiple-dimension protein identification technology;Washburn et al., 2001)procedure allows the sequencing of a large number of peptides in one putational methods then analyze all this peptide sequence information to identify the list of the proteins in the original ing this approach,more than1500proteins from a given sample can be identified in one MudPIT analysis(Jacobs et al.,2004;Peng et al.,2003).This is su Y cient for bacterial proteomics,but is inadequate for higher eukaryotes.Although the size of the eukaryotic proteome is unknown,it is likely to be on the order of12,000–20,000proteins.Thus the proteome must be prefractionated.A recent study using fractionation of the soluble proteins by gelfiltration revealed slightly more than5000proteins from the soluble extract of a mammalian cell line(Resing et al.,2004)but required hundreds of individual LC-MS analyses.In addition to capacity,di Y culties remain in sensitivity(dynamic range),resolving all of the protein isoforms,and in quantification,but innovations in instrument capabilities and protocols continue to move the state of the art forward at a rapid pace.A.B RIEF O VERVIEW OF S PECTROMETERS AND S PECTROMETRYTo understand the informatics issues,it is important to have a sense of the instruments and procedures that generate the data.The biological sample isfirst separated into component fractions by physical and chemical means.Each fraction has fewer constituents than the original sample,and hence is easier to analyze;however,then the many individual analyses must be recombined in some manner.As a typical example,the proteins in a given sample can be cleaved into short segments of1–200amino acids by a protease such as trypsin,which cleaves at a specific peptide bond(lysine or arginine).However,this cleavage is not perfect due to steric,hydrophobic,and other influences of adjacent amino acid residues. This is an example of the importance of understanding the underlying processes; protein identification algorithms have to model this cleavage step,and ap-proaches that incorporate these variations in detail perform significantly better than those that assume perfect cleavage.PROTEOMIC INFORMATICS133 Two-dimensional gel electrophoresis allows the creation of protein fractions that have a specific isoelectric point and molecular weight;this is often enough to isolate a specific isoform of a single protein from a complex mixture.The isolated protein spots are either physically cut out of the gel and digested,or digested in place and then removed.The digested fractions are generally analyzed using matrix-assisted laser desorption ionization time-of-flight(MALDI-TOF)mass spectrometry.However,as previously described,2D gels are being supplanted by higher-throughput methods of separating complex mixtures into fractions without electrophoretic separation.MudPIT involves enzymatic digestion of the entire complex mixture,followed by other fractionation steps.After enzymatic diges-tion,resulting peptides can be fractionated according to charge using strong cation exchange(SCX)chromatography.A reverse phase(RP)filtration step will further separate the peptides based on hydrophobicity.In either fractionation(as in many others),molecules with identical properties do not allflow through the chromatography medium at exactly the same rate but elute in a‘‘peak,’’with a variance due to di V usion or heterogeneous interaction with the chromatography ing the actual variances and other characteristics of the fractionation method in subsequent computation,rather than an unrealistically simple model, can lead to better performance in a variety of respects.Fractions containing peptides are then introduced into the mass spectrometer (via a spray or laser vaporization process)and accelerated by electric and magneticfields that act on the charged residues on each peptide.Excitation of the peptide ions may be employed using either a collision gas or high electric potentials at the mass spectrometer orifice to induce fragmentation of the peptide ion,providing useful information about the peptide sequence.The spectrometer’s fields are tuned to measure the time that a species takes to traverse a given distance in a given trajectory before being detected,or the peptides are trapped in a circulating pattern until a chosen time when molecules with specific properties are released for measurement.Of course even with highly precise instruments, noise occurs(as in any physical or electrical process)from imperfect equipment, chemical impurities,and human error.Spectrometers e V ectively measure the mass to charge(m=z)ratio,because that is what determines the acceleration of the molecules.The instrument reports the spectrum of intensities of the signal over a given m=z range.During the ionization process,a peptide can acquire or lose an additional hydrogen atom(a unit of charge),so a population of molecules of the same peptide will distribute over multiple charge states,each with a unique m=ing charge deconvolution algo-rithms,the mass of the peptides may be calculated from the measured m=z values.Tandem MS,or MS=MS uses two connected sequential spectrometers. Peptides are separated by m=z in thefirst spectrometer,then subjected to fragmentation and sent to a second spectrometer.The second spectrum provides information about each individual peptide,which can be used to determine its134RUSSELL et al.amino acid sequence.Shotgun proteomics methods often combine the LC-MS fractionation approach with the MS=MS peptide sequencing approach in a method called LC-MS=MS.Recall that the goals of these proteomics e V orts are:(1)to identify all the proteins in a complex mixture;(2)to determine the presence of protein isoforms such as post-translational modifications or splice variants;and(3)to quantify the concentrations of each protein or isoform.The inference of the answers to these questions from the kinds of a forementioned spectra is the task of informatics.IV.Identifying ProteinsBoth qualitative(identification only)and quantitative(identification and deter-mination of abundance)proteomics require that the masses detected by the spec-trometer be converted into information about the proteins in the original mixture.It is generally the case that information from the spectra is used to characterize the peptides that resulted from the enzymatic digestion;this characterization is in turn used to determine which proteins produced those peptides.There are two broad approaches to the task of peptide characterization: database-driven techniques and de novo sequencing.The database method matches observed peptide masses with calculations of the masses of peptides that would theoretically be produced by enzymatic digestion of all of the protein sequences from the organism under study.These theoretical calculations depend both on a model of the cleavage properties of the digestion enzyme and on a database that contains the complete set of sequences of the proteins in the organism(generally derived from the sequenced genome).De novo algorithms infer a peptide sequence from the spectra alone.For organisms whose genomes are not sequenced,de novo methods are the only option for proteomic analysis. This approach could also be used in identifying unexpected modifications.A.D ATABASE M ETHODS FOR P EPTIDE C HARACTERIZATIONThe database-searching algorithms in turn include three dominant ap-proaches:(1)peptide massfingerprinting,(2)sequence tag identification,(3)and MS=MS identification.Massfingerprinting involves matching peptide masses to theoretical digests calculated on the proteins in the database.Sequence tag identification involves generating a partial amino acid sequence from MS=MS fragmentation spectra and searching based on that partial sequence(and peptide mass information).The MS=MS full sequencing approach correlatesPROTEOMIC INFORMATICS135 experimentally derived fragmentation spectra with theoretical fragmentation patterns derived from a protein sequence database.We will now consider specific algorithms for each of these methods.Peptide massfingerprinting(PMF;also sometimes called protein mass mapping)is the mainstay technique for protein identification in gel-based prote-omics,in which2DE is used to separate proteins by isoelectric point and molecular weight prior to the MS analysis.The isolated protein spots are cut out of the gel and digested,after which the peptides are eluted and analyzed using MALDI-TOF mass spectrometry,providing a‘‘massfingerprint’’of the peptides. In PMF methods,peptides are characterized only by their masses,and the main algorithmic task is to match the set of masses to database protein sequences that could have generated them.Successful identification depends on high mass accuracy for the peptides, complete resolution of the proteins in the gel to avoid getting peptides from multiple proteins in a sample,and su Y cient detection of peptides by the spec-trometer.The procedure is only practical when the peptides were digested with highly specific enzymes such as trypsin.The peptide masses are then compared with the theoretical masses calculated for each protein in the database and a score is calculated representing the degree of matching.For a variety of reasons, it is always the case that some predicted masses will be missing from the spectrum and other unexpected masses will be present.For example,a covalent modifica-tion of the protein will cause the theoretical mass of a peptide to be missing,and an unexpected mass(with the additional mass from the modification)will be present.Contaminants are usually present,generating unexpected masses,or chemical processes can mask certain peptide signals.Most algorithms use a rudimentary matching score,which represents the overlap between expected and observed masses,such as PepSea(Henzel et al.,1993),MS-Fit(Clauser et al.,1999),PepFrag(Fenyo et al.,1998),and PepIdent(Wilkins and Williams, 1997).Others incorporate probabilistic models to account for the nonuniform distribution in peptide and protein molecular weights;these include(MOWSE) (Pappin et al.,1993)and ProFound(Zhang and Chait,2000).These algorithms are generally packaged with organism-specific protein sequence databases and are sometimes referred to as‘‘search engines,’’because they search a database for proteins that would produce the observed spectra.ProteinScape(Chamrad et al., 2003)is a meta-method that performs automated calibration and peakfiltering and searches across multiple PMF search engines(currently Mascot,MS-Fit, and ProFound).ProteinScape combines the results into a single score and calculates a statistical significance,using an expectation based on simulations. The tool FindPept(Gattiker et al.,2002)can be used to identify unmatched masses present in these spectra due to chemical noise,matrix peaks,and mod-ifications of the peptides introduced through sample handling and contaminating proteins.136RUSSELL et al.The other two peptide characterization techniques rely on MS=MS fragmen-tation analysis of peptides,using peptide sequence tags or correlating the MS=MS spectra with theoretical spectra calculated from sequence databases. Peptide sequence tag queries involve generating a partial amino acid sequence from the fragmentation spectra,followed by searching the sequence database with this partial sequence and an associated mass.Search programs using theoretical spectra score the experimentally derived fragmentation spectra by measuring the correlation of its theoretical fragmentation pattern with the exper-imental spectrum.The sequence tag and theoretical spectra searching techniques are better suited for analysis of complex mixtures than PMF.These methods can be applied to proteases or chemical methods with nonspecific cleavage, because they generate information about the sequence of the peptides,not just the masses.Thefirst publically available tool available for sequence tag searching was PeptideSearch(Mann and Wilm,1994;Mann et al.,1993)from Matthias Mann, which was designed primarily for lower-resolution data from triple quadrupole mass spectrometers,and allows searching with short sequences derived from ion ladders in peptide fragmentation spectra.More recent tools for this task include MS-Tag(Clauser et al.,1999),TagIdent(Wilkins et al.,1998),and GutenTag (http:==fi=GutenTag=index.html).When searching sequence tags for organisms with incomplete or unsequenced genomes,MultiTag(Sunyaev et al., 2003)may be useful,because it identifies homologous proteins from the genomes of related organisms.The spectrum of programs and algorithms for peptide identification using MS=MS spectra is quite large and constantly changing,from simple scoring algorithms to integrative systems built on top of the basic scoring methods.One of the most widely used scoring algorithms is SEQUEST’s XCorr(Yates et al., 1995),which uses cross-correlation to calculate a score between the experimental and theoretical spectrum for each peptide in the database within a user-specified tolerance of the parent mass.The result is a list of candidate peptides sorted by score.The top scoring peptide is chosen as the correct identification if it scores above a certain threshold,the level of which is somewhat subjective,because the score distributions vary according to mass of the parent peptide and charge state, as well as database size.To address the mass dependence,MacCoss et al.(2002) developed SEQUEST-Norm,which is normalized to be independent of peptide mass and protein database.Another popular program is MASCOT(Perkins et al., 1999),which is based on the MOWSE(Pappin et al.,1993)algorithm by incorporating probability based scoring and prebuilt peptide indexes.Currently SEQUEST and MASCOT are the most popular approaches to MS=MS spectra analysis,although neither is ideal.Both SEQUEST and MASCOT are distributed commercially,so the source code is not generally available.This makes it di Y cult to know exactly what the programs are doing,PROTEOMIC INFORMATICS137 to track changes from release to release,and to test all aspects of each approach independently.A recently developed open source software program for MS=MS identification,X!Tandem(Craig and Beavis,2003),matches peptide sequences with MS=MS spectra and identifies modifications using an iterative approach,in which the likely candidates are identifiedfirst from the total databases,followed by a search on the refined,smaller list of proteins to search for modifications.In a comparison of X!Tandem,Sonar MS=MS,and MASCOT,all three packages identified the same set of peptides,although with di V erent scores and expectation values(Craig and Beavis,2003).Several probability based scoring algorithms have emerged recently,such as SCOPE(Bafna and Edwards,2001),ProbID(Zhang et al.,2002),and OLAV (Colinge et al.,2003),all of which use stochastic models to improve the separation of correct from incorrect or random matches of expected to observed MS=MS spectra.SCOPE employs a two-stage stochastic model for matching spectra to peptides sequences.Thefirst step involves the generation of fragment ions from a precursor peptide using fragment ion probabilities derived empirically from a training set of expert-curated MS=MS spectra.The second step incorporates a model of instrument measurement error.OLAV,an algorithm based on signal detection theory,calculates a likelihood ratio score based on the degree of match-ing between experimental and theoretical spectra,and incorporates additional information about the match,such as parent mass and charge state.When compared to MASCOT using receiver operating characteristic analysis,OLAV demonstrated higher discrimination between correct and random matches (Colinge et al.,2003).As part of the RADARS(Field et al.,2002)package from Genomic Solutions(Ann Arbor,MI),developed by Beavis and co-workers,Sonar MS=MS(Field et al.,2002)calculates a correlation score using the inner product of the experimental and theoretical spectra,and incorporates expectation values in the calculation of a confidence score.Meta-methods are useful in this context too.The simple approach of comparing SEQUEST and MASCOT peptide identifications,and requiring a reasonable level of agreement between them,can be useful for improving confi-dence in identifications,and is generally used in our laboratory.SpectrumMill is a web-based workbench type of environment for extracting,searching,and visua-lizing LC-MS=MS data,compatible with mass spectrometers from multiple vendors.It was developed by Karl Clauser at Millennium Pharmaceuticals and is marketed by Agilent Technologies().Qscore(Moore et al.,2002)and PeptideProphet(Keller et al.,2002)are probabilistic systems built on top of other scoring systems and are designed to improve the separation of positive and negative identifications.Qscore was designed for SEQUEST score evaluation and is based on a model of random peptide matching,incorporating the fraction of distinct tryptic peptides matched in the database that are present in the identified protein.Keller and co-workers from the Institute for Systems138RUSSELL et al.Biology(Seattle WA)designed PeptideProphet to distinguish correct from incor-rect peptide assignments from SEQUEST searches using a machine learning approach called discriminant analysis,trained on a known set of validated MS=MS spectra.Similarly,Anderson et e a machine learning algorithm, called a support vector machine,trained on a set of validated identifications, using as input multiple scoring values from SEQUEST searches.Kislinger et al.(2003)developed PRISM,which is a systems approach rather than an algorithm,and addresses every step in the process,from subcellular fractionation and extraction of proteins to the clustering and annotation of the final protein list.Associated with the PRISM process is an algorithm called STATQUEST,which postprocesses the output of SEQUEST.STATQUEST performs a statistical analysis of peptide identification scores to estimate the accuracy of identifications,using an empirically derived probabilistic model that applies specifically to the PRISM process.Visualization andfiltering programs are often used to aid in the manual inter-pretation of results or initialfiltering of spectra prior to processing.CHOMPER, developed by Eddes et al.(2002)as well as DTAselect and Contrast,developed by Yates and co-workers(Tabb et al.,2002),aid in the validation of SEQUEST results by human experts,using a series of HTML-based output windows.These programs display the sequences of automatically identified peptides alongside the underlying MS=MS spectra,allowing experts to assess the reliability of the identification by eye.INTERACT(Han et al.,2001)is a similar tool developed by Jimmy Eng to collect and organize the large number of MS=MS spectra generated from large shotgun proteomics experiments.It is open source and freely available.B.D E N OVO P EPTIDE C HARACTERIZATIONThere are fewer options for de novo(non database)peptide sequencing from MS=MS spectra.Most algorithms enumerate and score sequence ladders made from the mass di V erences of the peaks,which should correspond to combinations of amino acid masses.Lutefisk(Taylor and Johnson,1997)uses a graph algorithm to enumerate all possible paths through the MS=MS peaks and scores the candidates using cross-correlation and an intensity based score.A de novo sequencing algorithm based on supervised machine learning,SHERENGA (Dancik et al.,1999),uses a set of validated test spectra and learns the relative intensities of ion types,which will be specific to the type of mass spectrometer;it can handle data from triple quadrupole,quadruple time offlight(QTOF),and ion trap ing this information,a list of ranked and scored se-quences is generated for the set of unknown spectra.CIDentify(Taylor and Johnson,2001)is a hybrid approach that takes as input the identified sequencePROTEOMIC INFORMATICS139 candidates from Lutefisk and performs a homology-based database search.This approach is applicable to source organisms without sequenced genomes,so long as sequence from a related organism is available.C.F ROM P EPTIDES TO P ROTEINSRecall that massfingerprinting methods attempt to map from collections of proteolytic peptide masses to the particular proteins that could have contained them.The addition of sequence information makes this problem more tractable and less subject to error.However,the protein identification from peptide data is still potentially ambiguous,and all existing algorithms make at least some errors in this step.In general the programs that do MS=MS identification of peptides also provide database-driven matching of identified sets of peptides to specific proteins.These programs function much the same way that the massfinger-printing programs do,and are often derived from them.A small number of peptides(sometimes only one)can be enough to uniquely identify a particular protein,and these programs make protein identification calls even when the majority of peptides that are predicted from the digestion of that protein are not found.ProteinProphet from Institute of Systems Biology,performs such a task, assembling peptide identifications with associated probabilities into protein iden-tifications and derived probabilities.One di Y culty in this task arises because of the large abundance of alternative splicing,protein isoforms,and database redundancies.Identified peptides may belong to more than one related protein. Generally the aforementioned programs integrate this step into their processing. ProteinProphet is unusual in that it is a stand-alone program that takes the peptide identification output of MASCOT,SEQUEST,or another program as its input,and only does protein identification.Isoform Resolver is a similar program,but it assigns the protein identification from the peptide sequence, independent of the search program assignment(Resing et al.,2004).V.Post-hoc Validation of Protein Identification Program Output The most popular approaches to protein identification(SEQUEST and MASCOT)both have fairly high false-positive and false-negative rates(MacCoss et al.,2002;Moore et al.,2002).In recent studies,manual analysis and direct spectral comparison have shown that,at their high confidence cuto V s, SEQUEST and MASCOT both miss at least half of potentially identifiable MS=MS spectra.Furthermore,even at these high confidence cuto V s,they make incorrect assignments29–45%of the time(Keller et al.,2002;MacCoss et al.,。

激光剥蚀电感耦合等离子体质谱法测定高纯金中杂质元素

激光剥蚀电感耦合等离子体质谱法测定高纯金中杂质元素

激光剥蚀电感耦合等离子体质谱法测定高纯金中杂质元素摘要:本文探究了激光剥蚀电感耦合等离子体质谱法(LA-ICP-MS)测定高纯金中的杂质元素。

起首,通过样品前处理、ICP-OES 和XRF等技术,确定了高纯金样品中的杂质元素含量。

然后,使用LA-ICP-MS法对样品进行测量,并使用外标校正法进行结果修正。

结果表明,该方法具有高准确性、高灵敏度和较低的检出限,可用于高纯金中微量元素的精确测定。

关键词:激光剥蚀;电感耦合等离子体质谱法;高纯金;杂质元素;外标校正法引言:高纯金是一种重要的材料,广泛应用于电子、半导体和高温超导等领域。

由于其高纯度,通常状况下仅允许少许杂质元素存在。

因此,准确测定高纯金中杂质元素的含量是分外重要的。

传统的测量方法通常使用ICP-OES、ICP-MS和XRF等技术,但这些方法通常需要破坏样品结构或需要复杂的前处理过程。

近年来,激光剥蚀电感耦合等离子体质谱法(LA-ICP-MS)已经成为测定高纯金中杂质元素含量的一种新方法。

与传统方法相比,LA-ICP-MS具有分外好的灵敏度和准确性,而且不需要破坏样品结构。

本文旨在探究LA-ICP-MS测定高纯金中杂质元素的适用性和精度。

试验与方法:试验接受电感耦合等离子体质谱仪(Agilent 8800),激光系统为NewWave Research UP193FX,激光参数如下:重复频率1 Hz,能量密度100 mJ/cm2,脉冲宽度20 ns。

为了减小激光剥蚀造成的影响,使用了2 mm的方形钨丝放置在样品底部,使样品与钨丝成短距离的垂直距离。

样品前处理接受洛氏硫酸提取法和预处理程序(Agilent Technologies)。

ICP-OES和XRF测量接受扫描电子显微镜(SEM)和能谱分析仪(EDS)协作实现。

结果与谈论:通过样品前处理、ICP-OES和XRF等技术,确定了高纯金样品中的杂质元素含量。

结果表明,高纯金样品中主要杂质元素为铁、镍、银、钴和铬等,其含量均低于10 ppm。

KitAlysis高通量24剂量熔化催化剂光伏反应筛选套件说明书

KitAlysis高通量24剂量熔化催化剂光伏反应筛选套件说明书

KitAlysis™ High-Throughput 24-CatalystPhotoredox Reaction Screening KitCatalog Number KITALYSIS-PHOStorage Temperature 20–25 ︒CTECHNICAL BULLETIN Product DescriptionThe KitAlysis™ High-Throughput 24-CatalystPhotoredox Reaction Screening Kit enables chemists toquickly and efficiently screen reaction conditions formany photocatalyzed transformations.Quick identification of optimal conditions allows fasterscale-up of the desired synthetic transformation. Allrequired chemicals come pre-weighed for ease of use.Each KitAlysis High-Throughput 24-CatalystPhotoredox Reaction Screening Kit contains2-individual screening sets. Each screening set hascomponents to run 24 unique photocatalysis reactions:∙24 ⨯ 1 photocatalysts (provided)∙10 μmol substrate(s), reagent(s), co-catalyst(s)(user supplied)∙ 4 solvents (DCM, DCE, DMF, and MeCN)(provided)Access /kitalysis for detailedstep-by-step Protocols and Scale-up Guide. TheProtocols include an introductory video, instructions forthe lab ware, and work-up/internal standard additionprocedures.A downloadable spreadsheet for stock solution recipesfor each screening set is available from the step-by-step Protocol.The spreadsheet provides:∙Calculations for substrate recipes based on themolecular weight of user supplied substrates∙Quick directions for experienced users∙Print button that allows you to take experimentalrecipes to the lab∙Contains all experimental information, which caneasily be saved as a pdf file and appended toelectronic laboratory notebooksScreening Kit Components∙Two screening sets individually sealed undernitrogen in mylar bags. Each bag includes:o24 pre-weighed (1 μmol) photocatalysts inglass vials loaded with stir bars and topped withcap mat, see Figures 1 and 2o 2 ⨯ 4 mL reaction vials for preparing substratestock solutions∙Ampule boxes containing degassed, anhydrousliquids:o 4 ⨯ 2 mL DCMo 4 ⨯ 2 mL DCEo 4 ⨯ 2 mL DMFo 4 ⨯ 2 mL MeCN∙Two new KitAlysis 24-Well Reaction BlockReplacement Films∙8 new stir bars for substrate stock solution vials∙Biphenyl Internal Standard (30 mg)Note: While the above scheme depicts a generic decarboxylativecoupling reaction, KitAlysis High-Throughput 24-CatalystPhotoredox Reaction Screening Kit can be used for anyphotocatalytic transformations.2 Figure 1. CatalystsFigure 2.Position of CatalystsReagents and Equipment Required, but Not Provided.∙ 10 μmol substrate(s), reagent(s), co-catalyst(s) ∙ Photo KitAlysis Starter Kit (Z742612)∙ Nitrogen (or argon): from hood line or tank ∙ Pipette (0–100 μL) and tips∙ 4 (1 mL) syringes with long needles ∙ 2 stir plates∙ HPLC vials, 96-well HPLC auto sampler block, orTLC plates.Specially designed KitAlysis Labware allows screening set up in a hood without the use of a glove box.∙ KitAlysis Benchtop Inertion Box (Catalog NumberZ742064)Instructions for the Labware can be accessed in the Articles section of the website product display page for the kit.Precautions and DisclaimerThis product is for R&D use only, not for drug,household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.Storage/StabilityStore the KitAlysis High-Throughput 24-Catalyst Photoredox Reaction Screening Kit at room temperature.KitAlysis is a trademark of Sigma-Aldrich Co. LLC.CK,MAM 01/19-1©2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Aldrich brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for theirparticular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at and/or on the reverse side of the invoice or packing slip.。

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PROPERTIES FOR MODULATION SPECTRAL FILTERINGQin Li and Les AtlasDepartment of Electrical Engineering, University of WashingtonBox 352500, Seattle, WA 98195-2500ABSTRACTA two-dimensional representation,the “modulation spectrum,” where modulation frequency exists jointly with regular Fourier frequency, or other filter channel index, has been previously investigated. Accurate modulation filters would offer, for example, new approaches for signal separation and noise reduction. However, a filtering operation on modulation frequency components has yet to be carefully defined. Most previous studies on modulation filtering assumed that the amplitude modulation envelope is real and non-negative, which has recently been shown to be incorrect. Distortions appear when the non-negative envelope assumption fails. Beginning with a more appropriate envelope assumption that allows the envelope to go negative,we propose three properties which modulation filtering systems should satisfy.Any modulation filtering method which satisfies these properties will yield distortion-free results. An implementation of modulation filtering based on a short-time Fourier transform followed by independent coherent demodulation for each frequency channel is then proposed. Satisfaction of the properties is confirmed and an example result of modulation filtering on speech signal is illustrated.1. INTRODUCTIONSome simple signals can be represented by a modulation model, where a low-frequency modulator signal, often referred to as an amplitude modulator(AM) or envelope, multiplies a high-frequency carrier signal. The concept “modulation frequency” is then associated with a Fourier transform of the modulator. Many physiological and psychoacoustic studies (e.g. [1-3]) have shown the importance of modulation features in audition. A more general signal model,which is an invertible two-dimensional representation for modulation signals, where modulation frequency exists jointly with regular Fourier frequency, has been previously described [4, 5]. However,a filtering operation on modulation frequency components has yet to be well defined. Most previous studies on modulation filtering [2, 4-8]assumed that the modulation envelope is real and non-negative. The magnitude of a complex sub-band signal or a Hilbert envelope (magnitude of an analytic signal) was used to separate a modulator and a carrier. Modulation filtering based on this non-negative envelope assumption usually produces unwanted distortions and ineffective filtering [9]. In this paper, we propose three key properties for modulation filtering,along with a new approach which satisfies these properties.Modulation filtering involves decomposition of modulator and carrier, which is often referred to as amplitude- and frequency-modulation (AM-FM) decomposition in many time-frequency analysis contexts [10-12]. Since the carrier in our signal model may have a time varying instantaneous frequency (IF), “carrier”often refers to an FM part and “modulator” refers to an AM part.The modulator-carrier decomposition is also called a “detection operation”in previous studies [4,5, 9, 10].2. MODULATION FILTERINGWe first propose a signal model with conditions on modulatorsand carriers. A modulation filtering principal is then based upon properties, analogous to, yet distinct from linear time-invariantfiltering, that a well defined system should satisfy.2.1 Signal ModelConsistent with the previously discussed two-dimensional model,a fixed short-time transform or filter bank is used to decompose a broadband input signal into presumed single component models11()()()()N Nn nn nnx t s t m t c¦¦t, (1) where N is the number of modulation components or number ofsub-bands;and are the modulator and carrier, respectively, for the n th component or sub-band. As will bediscussed later,one of the three proposed properties will directlyaddress issues resulting from any lack of fit between this signalmodel and sub-band locations in frequency.()nm t()nc tBeginning with a simple, single carrier model, an amplitudemodulated signal can be expressed as the product of an envelopeand a carrier,()()()n n ns t m t c t.(2)It is straightforward that the modulation frequency for this signalis the Fourier transform of the modulator,()nm t^`()()()jn n nj tM e F m t m t eZ ff³dt Z. (3)For any given signal, there are an infinite number of modulator-carrier pairs satisfying (2) without other constraints [13]. We willbegin to restrict this infinite set below and continue to restrict itmore with the properties in Section 2.2.To be consistent with previous studies of invertible modulationspectral analysis [4, 5],the following conditions are imposed onthis modulation model.x()ns t is the observed real or complex signal.x is a high-frequency carrier signal. It may be real orcomplex. If it is complex, it must be a phase signal,i.e.; or if it is real, it must be in a form ofn. The time derivative of is instantaneousfrequency(IF) of the signal()nc t()()n j tnc t e I()cos()nc t tI()ntI()ns t.This carrier frequency mustbe limited to the same frequency range of the signal()ns t.x is a real or complex low-frequency modulator with no significant frequency content close to or above the carrier frequency.()n m t Previous modulation spectral analysis and filtering methodsgenerally assume that the modulator is real and non-negative, which has been shown invalid in most cases [9, 10]. On the other hand,we have to realize that decomposing a signal into complex modulator and carrier is a challenging problem because such a decomposition is not unique in general [10, 13].Nevertheless, for specific applications or signals, we are able to restrict the possibilities for modulator-carrier decomposition. For example, if a signal is approximately stationary within some time window, a constant frequency carrier, i.e. c c t ()n m t ()cos n t Z or ()c j t n c t e Z , can be used.By estimating the mean frequency of a sub-band signal, we are able to separate modulator and carrier uniquely. Precisely estimating the IF of a time-varying signal with a complex modulator is still an open question and beyond the scope of this paper. Related discussions about the IF estimate can be found in [10].2.2 Properties of Modulation FilteringFor simplicity,we begin with the single component modulation signal given by (2). For this simple case, a modulation filtering system L can be expressed as a linear time-shift invariant (LTI)system T on the modulator ,()n m t ^`^`>@()()()()()()n n n n n L s t c t T m t c t m t h t , (4)in the time domain, or^`^`>@()()()()()(),n n n n n L S C T M C M H Z Z Z Z Z Z (5)in the frequency domain, where and ()h t ()H Z are the impulse and frequency response, respectively, of the system T . It should be noted that while T is L TI, the overall modulation filtering operation L is not LTI in general. However, for certain cases, the modulation filtering system L can still obey superposition and other desirable properties. These properties are required for the filtering system L to behave well, e.g., filtering on modulation effectively, working properly for multi-component inputs, and yielding no distortion. We hereby propose three basic properties that the modulation filtering system L is desired to satisfy.1. S uperposition , where modulation filtering on the linear combination of two modulation signals yields the same result asthe linear combination of modulation filtering on each signal:^`^`^`1212()()()()L as t bs t aL s t bL s t . (6) Modulation signals may have multiple components and a linear relationship should be satisfied among those components.Linearity in (6)is valid only under some conditions. For example,given a two-component modulation signal 12()()()s t s t s t ,where 111()()()s t c t m t and 222()()()s t c t m t , we should be able to find ^`11(),()c t m t and ^`22(),()c t m t from ()s t alone. One sufficient condition is that 1()s t and 2()s t have no overlap in frequency, so that ()s t can be easily split into 1()s t and 2()s t .This condition can be satisfied by a fixed ideal filter which separates the components. Thus, we use the word “superposition”to distinguish this conditional linearity from the well-known general linearity concept. Satisfaction of thisproperty depends on signal and filter band design. For example,in order to separate all modulated components, the bandwidth of the sub-bands should be small enough so that only one or zero modulation component is contained in each sub-band.2.Frequency-shift invariant property, where a frequency shift of the input signal results in the same frequency shift of the output:^`^`212010if ()(),then ()().S L S S L S Z Z Z Z Z Z (7)This property is required by the goal of modulation filtering.Because modulation filtering is acting only on the modulator as in (4)and (5), it should yield same results no matter where the carrier frequency is. Obviously, the frequency-shift invariant property holds if and only if carrier detection or instantaneous frequency estimation is frequency-shift invariant.3.Linear time-shift invariant for modul ator property, which is simply the common LTI property for the modulator:>@^`^`^^`^`12124343()()()()()()();and if ()()()(),then()()()()L am t bm t c t aL m t c t bL m t c t m t c t L m t c t m t c t L m t c t W W `. (8)This property holds if and only if the system T in (4) and (5) is LTI.3. IMPLEMENTATION OF MODULATION FILTERINGA number of modulation analysis and filtering techniques are described in literature, for example the approaches used in [2, 4-8, 11, 14]. These approaches first use a filter bank or a short-time Fourier transform, followed by a magnitude or Hilbert envelope to separate the modulator and carrier; then analysis or filtering is performed on the modulator. A problem with these existing methods, as reported by Ghitza [14], is that these modulation filters show considerably less stop-band attenuation than they were designed for.In this section, we propose and assess a method of modulation filtering in which the short-time Fourier transform (STFT) is again used as a filter bank. Instead of taking a subsequent magnitude,coherent demodulation is used, within each sub-band,to separate the modulator and carrier.3.1 Coherent Modulation FilteringFigure 1. System for coherent modulation filtering. The thicker lines indicate the time functions are STFT sub-band signals. The superscript prime denotes output signals.Referring to figure 1, the major and the most important feature distinct from the previous methods is the step of coherent demodulation, which is done independently for every STFT sub-band.A carrier frequency ()inst f t is first detected for each sub-band; then coherent demodulation is performed on each sub-band by multiplying , where is the integral of carrier frequency with time,()c j t e I ()c t I 0()()tc inst t f t I ³dt .(9)If the carrier frequency is constant, this coherent demodulation step simply shifts the sub-band signal down to a low-frequency band. In other words, the carrier frequency detection and coherent demodulation replace the magnitude or Hilbert envelope used within the previous definitions.To complete the coherent modul ation fil tering system an L TI filter is applied on each sub-band signal followed by restoring the carrier and then combining sub-bands via an inverse STFT.As will be shown below, all three desirable properties,as discussed in Section 2.2, are satisfied by this system.3.2 Satisfaction of the Properties1.Superposition property. As stated in section 2, this property depends on appropriate filter bank design. Superposition is achieved by adjusting the width of sub-bands, so that there is no more than one modulation component within one sub-band. Note frequency overlap of the filter bank is allowed. For example, for a speech signal,the pitch and its harmonics can be assumed to be distinct carriers. In this case,the sub-band width should be no larger than pitch frequency. A demonstration of the superposition property for a synthetic signal is shown in figure 2a.2.Frequency shift invariant property. For a single component modulation signal, this property can be easily satisfied, since most IF estimation methods are frequency-shift invariant. In order to keep the superposition property for multiple components signals, we have to use a filter bank to break a signal into sub-band signals.This inevitably increases the difficulty of carrier frequency detection. In particular, when a carrier frequency is located near the boundary of two sub-bands, the carrier frequency tends to be misdetected.This misdetection problem can largely be solved by using a filter bank with overlapped sub-bands. For example, in our test implementation, an overlapping Hann window was applied within the STFT, resulting in 75% overlap in frequency between consecutive sub-bands. Each carrier component will appear in the main lobes of 4 sub-bands. Assuming the carrier frequency can be correctly detected, one modulated component will be decomposed, by the overlapping STFT, into 4 sub-components41()()()()()i i s t c t m t c t m t ¦.(10)The LTI property for the modulator, detailed below, guarantees that filtering on the sub-components in (10) yields the same result as filtering on the whole, namely^`^`^`^`4141()()()()()()()()()i i i i L s t c t T m t T m t c t c t T m t c t T m t ­½®¾¯¿¦¦(11)In the worst case, where the carrier frequency of a modulation component sits right at the boundary of two sub-bands, the strength of the carrier frequency becomes zero and will be misdetected. But this sub-component only contributes relatively small energy to the reconstructed signal. Other sub-components in neighbor sub-bands contribute the bulk of the energy to the reconstruction.A demonstration of this worst case is shown in figure 2b, where the sub-band has a bandwidth of 400 Hz with 75% frequency overlap. The carrier frequency is 2200 Hz, which sites right at the boundary of two sub-bands. As it is shown, 40dB suppression is achieved, while the filter is designed for 60 dB suppression, for low-pass modulation filtering, Although this is not perfect, 40dB suppression in modulation is well beyond human auditory perception [3].3.Linear-time shift invariant for modul ator property. Since the filtering step in figure 1 is L TI, the L TI property is easily satisfied for the modulator. An illustration of this property is shown in figure 2c. However it should be noted that proper window overlap in the STFT, needs to be chosen to avoid aliasing in sub-bands. For example, 75% overlap is needed for aHann window.Figure 2. Examples of property satisfaction. In all three panels,solid lines show inputs and dashed lines show modulation filtered outputs. (a) Test of the superposition property.Modulation component 1 consists of a 2120 Hz carrier and a 15Hz modulator; component 2 consists of a 3170Hz carrier and a 40 Hz modulator. A low-pass modulation filter at 30 Hz is applied on all sub-band signals.More than 60 dB suppression is achieved for the second component. (b) Test of the frequency-shift invariant property.The input signal consists of a 2200 Hz carrier, which falls at a boundary of two sub-bands, with a 25 Hz modulator.Low-pass modulation filtering is performed at 15 Hz and about 40dB suppression is achieved (c) Test of the modulator linear time-shift invariant property. The input signal consists of a 2120 Hz carrier and two sine wave modulators at 15 and 40 Hz. Low-pass modulation filtering is performed at 30 Hz and about 60 dB suppression is achieved for the 40 Hz modulator.4. EXAMPLEWe applied our coherent modulation filtering method to a speech signal. As shown in figure 3a, a segment of speech signal has a pitch frequency of about 100 Hz. To keep the superposition property, the STFT had sub-band width of 100 Hz with 75% overlap between consecutive sub-bands. Thus the modulation frequency range is from -50to50 Hz. A time-varying IF was estimated in each sub-band, corresponding to the time-varying pitch frequency. Note that the speech segment in figure 3a evolves in time between three different voiced sounds.Extreme low-pass output (figure 3b) from the proposed coherent modulation filtering method yields a flat envelope and equalizes the difference for the three voiced segments as desired; while the low-pass from the incoherent method [4] does not produce an flat envelope and produces distortion in the fine structure (figure 3c). Similar problems are seen for other previous incoherenttechniques.Figure 3. Example of modulation filtering on speech. (a) The original speech signal; (b) Output of a low-pass modulation filter (coherent method) with 1.4 Hz corner;(c) Output of a low-pass modulation filter (incoherent method) with 1.4 Hz corner.The above modulation low-pass effect seen on speech demonstrates that new types of predictable signal modifications are indeed possible with modulation filters.5. CONCLUSIONWe have defined three necessary properties for modulation filtering systems. Any modulation filtering method satisfying these three properties will potentially yield effective and distortion-free filtering results. We also implemented a new modulation filtering method,applicable to broadband signals, using a fixed STFT as a filter bank and coherent demodulation as a detection operation within each sub-band. It has been shown that the new method is able to satisfy all three properties. The preliminary test on a speech signal confirms the accuracy of this coherent approach. Accurate modulation filters,when designed for particular applications, offer new approaches for signal separation and noise reduction. Signals which overlap heavily in standard Fourier frequency often differ in modulation frequency extent. With accurate modulation filtering, these differences could be used as a basis for modulation frequency enhancement of the desired signal or for other signal modifications.This work was supported by the Office of Naval Research. We acknowledge helpful conversations with Dr. Bishnu Atal and Steven Schimmel of the University of Washington.6. REFERENCES[1]R. McEachern, "How the ear really works," in Proceedingsof the IEEE-SP International Symposium,Time-Frequency and Time-Scale Analysis, Victoria, BC, Canada, 1992.[2]Z. M. Smith, B. Delgutte, and A. J. Oxenham,"Chimaeric sounds reveal dichotomies in auditory perception,"Nature,416, pp. 87-90, 2002.[3] B. C. J. Moore, "Masking in the human auditory system," in Col lected Papers on DigitalAudio Bit-Rate Reduction, N. Gilchrist and C. Grewin,Eds., 1996, pp. 9-19.[4]L. E. Atlas and M. S. Vinton, "Modulation frequency and efficient audio coding," in Proceedings of the SPIE, 2001.[5]J. K. Thompson and L. E. Atlas, "A Non-Uniform Modulation Transform for Audio Coding with Increased Time Resolution," in Proceedings of IEEE ICASSP, Hong Kong, 2003. [6]S. Greenberg and B. E.D. Kingsbury, "The modulation spectrogram: in pursuit of an invariant representation of speech,"in Proceedings of the IEEE ICASSP, 1997.[7]R. Drullman, J.Festen,and R. Plomp,"Effect of temporal envelope smearing on speech reception,"J. Acoust.Soc. Am.,95, pp. 1053-1064,1994.[8]T. Arai, M. Pavel, H. Hermansky,and C. Avendano, "Intelligibility of speech with filtered time trajectories of spectral envelopes," in Proceedings of the ICSLP, 1996.[9]L. E. Atlas, Q. L i,and J. K.Thompson, "Homomorphic Modulation Spectra," in Proceedings of the IEEE ICASSP, Montreal, Canada, 2004.[10]Q.Li andL. Atlas, "Over-Modulated AM-FM Decomposition," in Proceedings of the SPIE, Denver, 2004. [11]A. Rao and R.Kumaresan, "On decomposing speech into modulated components,"IEEE Transactions on Speech and Audio Processing,8, pp. 240-54, 2000.[12]T. F. Quatieri,T.E. Hanna, and G. C. O'L eary, "AM-FM separation using auditory-motivated filters,"IEEE Transactions on Speech and Audio Processing,5, pp. 465-80, 1997.[13]P. J. L oughlin and B.Tacer, "On the amplitude- and frequency-modulation decomposition of signals,"Journal of the Acoustical Society of America,100, pp. 1594-601, 1996.[14]O. Ghitza, "On the upper cutoff frequency of the auditory critical-band envelope detectors in the context of speech perception,"Journal of the Acoustical Society of America,110, pp. 1628-1640,2001.。

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