γδT细胞、Th17细胞和IL-17在慢性乙型肝炎患者外周血中表达的意义
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根据T细胞表面受体(TCR)的不同,T细胞分为 aBT细胞和y&T细胞a|3T细胞在获得性免疫应 答中发挥重要作用，而垃T细胞由于其分布特点和 免疫应答的非主要组织相容性复合体(MHC)限制 性，在固有性免疫中发挥着独特的作用MT细胞 在成年人外周血中仅占0.5%~5%.主要分布于黏膜 和皮下组织，如在人小肠上皮淋巴细胞(1EL)中占 10%~ 18%.人大肠IEL中占25%~37%，小鼠IEL中 占50% 2。黏膜和上皮组织是抵抗病原体入侵的
中华生物医学工程杂志2021年4月第27卷第2期 Chin J Biomed Eng, April 2021, Vol. 27, N().2
of 20 health checkup subjects. How cytometry was ust*(l to measure the counts of peripheral blood y8T an(i Th 17 cells. The serum IL-17 levels in each group was measured by EIJSA. Pearson correlation was used to analyze the correkition between percentages of peripheral y8T and Th 17 cells and the serum levels of IL-17 in the four groups of subjects. Results In healthy subjects, AsCs, mild and moderate-to-severe CHB patients, the presentation levels ofy8T cells were ( 1.258±0.135)%, (2.178±0.195)%, (4.160±0.070)% and (7.058± 0.970)%, respectively, with significant difference among groups (F=498.35. P二0.00()) : the presentation levels of Th 17 cell were (1.252±0.155)% , (1.714±0.103)% , (2.338±0.234)% and (3.826±0.488)% , respectively, with significant difference among groups ( F=310.65, /^=0.000) ; the levels of IL - 17 were (16.307± 19.25) pg/ml, (92.706±16.85 ) pg/ml, (147.635±6.32) pg/ml・ and (391.787±28.52) pg/ml, with significant difference among groups (F=349.41, /J=0.()00). Pearson correlation analysis showed positive correlations between the peripheral blood percentage of y8T cells and Th 17 cells in CHB patients (厂=0.978, /J=0.000), between the percentage of y6T cells and IL-17 level (r=0.959, P=0.000) , and also between the percentage of Th 17 cells and IL-17 level (r=0.954, /=().()00). Conclusion yST cells. Th 17 cells and IL-17 may be involved in the immune response and profess of tissue damage caused by HBV infeetion, and are closely related to the severity of CHB.
[Abstract] Objective To investigate the peripheral blood presentation of yST cells, Th 17 cells and IL-17 in patients with chronic hepatitis B (CHB), and to determine the potential role of yST cells, Th 17 cells and IL - 17 in pathogenesis of CHB. Methods Randomly included in this study were 20 asymptomatic chronic carriers of HBV ( AsC), 20 patients with mild CHB, 20 with moderate-to-severe CHB who stayed in our hospital or visited our clinics between November 2015 and April 2016, as well as a contemporary cohort
Significance of 78T cells, Thl7 cells and IL-17 presentation in peripheral blood of patients with chronic hepatitis B Jin Hongmei1, Wang Yashir, Yuan Zhenxin ", Wu Jiafeng , Wang Keqiang4 Department oj Blood Transfusion , Second Affiliated Hospital of Shandong First Medical University, Tai * an, Shandong 271000, China; 'Department of laboratory Medicine. Tai' an Central Hospital, Tai' an, Shandong 271000, China; Department()/ laboratory Medicine Tai' an Cancer Institute, Tai * an , Shandong 271000, China ; 4Department of laboratory Medicine, Second Affiliated Hospital of Shandong First Medical University, Tai' an. Shandong 271000, China Corresponding author： Wang Keqiang, Email: ivkqsd@163. com
第一道防线细胞在黏膜及上皮组织中的高比
例分布，提示7&T细胞在抗微生物、寄生虫及肿瘤 免疫方面起重要作用a： Th 17细胞是a0T细胞的 一个亚型，是•种分泌促炎性细胞因子的CD4 T细 胞亚群，广泛参与慢性炎症、自身免疫性疾病、肿瘤 以及感染性疾病的发生与发展，主要分泌细胞因子
IL-17.ROR71是其关键的核转录因子“叫1L-17是 HT细胞和Thl7细胞的重要效应因子，具有强大的 促炎作用”慢性乙型肝炎(CHB)是一种常见的传 染病.严重危害人民的身体健康，当前各种药物和 治疗方法均不能有效清除已经整合到肝细胞内的 乙型肝炎病毒本研究用流式细胞术(FCM)及 ELISA检测CHB患者外周血中yST细胞、Thl7细胞 的数量及细胞因子1L-17的表达情况，探讨78T细
[Key words] Hepatitis B, chronic; y8T cells; Th 17 cells; Interleukin 17; Cytokines Fund Program: Natural Science Foundation of Shandong Province (ZR2020MH312) ; Shandong Provincial Health Science and Technology Assoc iation Project (2016BJ0003) ; Tai' an Science and Teclinolog\, Innovation Development Project (2018NS01 16 ,2020NS129) DOI ：10. 3760/cma. j. cnl 15668-20210125-00016
162 •
中华生物医学工程杂志2021年4月第27卷第2期 Chin J Biomed Eng, April 2021, Vol. 27, No.2
•新技术与临床•
y5T细胞.Th 17细胞和IL-17在慢性 乙型肝炎患者外周血中表达的意义
晋红梅「王雅姝：苑振新'吴家锋4王克强* 「山东第一医科大学第二附属医院输血科，山东泰安271000；:泰安市中心医院检验
【关键词】乙型肝炎.慢性；2T细胞；Thl7细胞；白细胞介素17；细胞因子类 基金项目：山东省自然科学基金(ZB2O2OMH312)；山东省保健科技协会计划课题(2016BJ0003 )； 泰安市科技创新发展项目(2018NS0116、2020NS129) DOI: 10. 3760/ema. j. cnl 15668-20210125-00016
科，山东泰安271000； 3泰安市肿瘤防治院检验科，山东泰安271000； 4山东第一医科 大学第二附属医院检验科，山东泰安271000 通信作者:王克强，Email: wkqscl@
【摘要】目的了解RT细胞,Thl7细胞和IL-17在慢性乙型肝炎(CHB)患者外周血中的表达情 况，探讨RT细胞、Thl7细胞和IL-17在CHB发病机制中可能的作用：方法随机选取2015年11月至 2016年4月本院门诊或住院收治的慢性无症状HBV携带者(AsC)20例、CHB轻度患者20例、CHB中和 重度患者20例、同期健康体检者20例用流式细胞仪检测外周血中2T细胞和Th 17细胞的数量,用 ELISA法检测各组血清中IL-17细胞因子的含量应用Pearson相关分析4组研究对象外周血中询T、 Thl7细胞的百分率及血清中IL.-17水平三者之间的相关性结果健康人、AsC、CHB轻度患者和CHB 中、重度患者Y$T细胞的表达量分别为(1.258±0.135)%,(2.178±0.195)%,(4.160+0.070)%,(7.058+ 0.970)%，差异具有统计学意义(F=498.35. P=0.000) ；Thl7细胞的表达量分别为(1.252±0.155)%、 (1.714±0.103)%、(2.338±0.234)%、(3.826±0.488)%，差异有统计学意义(F=310.65, P=0.000) ；IL-17 的 浓度分别为(16.307± 19.25) pg/ml、(92.706+16.85) pg/ml、( 147.635±6.32) pg/ml .(391.787+28.52) pg/ml, 差异有统计学意义(F=349.41/=0.00()) Pearson相关性分析显示,CHB患者外周血中沦「细胞和Thl7 细胞的百分率存在正相关(r=0.978, P=().()()0) ; 2T细胞的百分率和II.-I7存在正相关(r=0.959, P= 0.000)；Th 17细胞的百分率和IL-17存在正相关(r=0.954.P=0.000)=结论 2T细胞、Thl7细胞和IL-17 可能参与『机体感染乙型肝炎病毒后引起的免疫反应和组织损伤过程.并与CHB的重症化密切相关
中华生物医学工程杂志2021年4月第27卷第2期 Chin J Biomed Eng, April 2021, Vol. 27, N().2
of 20 health checkup subjects. How cytometry was ust*(l to measure the counts of peripheral blood y8T an(i Th 17 cells. The serum IL-17 levels in each group was measured by EIJSA. Pearson correlation was used to analyze the correkition between percentages of peripheral y8T and Th 17 cells and the serum levels of IL-17 in the four groups of subjects. Results In healthy subjects, AsCs, mild and moderate-to-severe CHB patients, the presentation levels ofy8T cells were ( 1.258±0.135)%, (2.178±0.195)%, (4.160±0.070)% and (7.058± 0.970)%, respectively, with significant difference among groups (F=498.35. P二0.00()) : the presentation levels of Th 17 cell were (1.252±0.155)% , (1.714±0.103)% , (2.338±0.234)% and (3.826±0.488)% , respectively, with significant difference among groups ( F=310.65, /^=0.000) ; the levels of IL - 17 were (16.307± 19.25) pg/ml, (92.706±16.85 ) pg/ml, (147.635±6.32) pg/ml・ and (391.787±28.52) pg/ml, with significant difference among groups (F=349.41, /J=0.()00). Pearson correlation analysis showed positive correlations between the peripheral blood percentage of y8T cells and Th 17 cells in CHB patients (厂=0.978, /J=0.000), between the percentage of y6T cells and IL-17 level (r=0.959, P=0.000) , and also between the percentage of Th 17 cells and IL-17 level (r=0.954, /=().()00). Conclusion yST cells. Th 17 cells and IL-17 may be involved in the immune response and profess of tissue damage caused by HBV infeetion, and are closely related to the severity of CHB.
[Abstract] Objective To investigate the peripheral blood presentation of yST cells, Th 17 cells and IL-17 in patients with chronic hepatitis B (CHB), and to determine the potential role of yST cells, Th 17 cells and IL - 17 in pathogenesis of CHB. Methods Randomly included in this study were 20 asymptomatic chronic carriers of HBV ( AsC), 20 patients with mild CHB, 20 with moderate-to-severe CHB who stayed in our hospital or visited our clinics between November 2015 and April 2016, as well as a contemporary cohort
Significance of 78T cells, Thl7 cells and IL-17 presentation in peripheral blood of patients with chronic hepatitis B Jin Hongmei1, Wang Yashir, Yuan Zhenxin ", Wu Jiafeng , Wang Keqiang4 Department oj Blood Transfusion , Second Affiliated Hospital of Shandong First Medical University, Tai * an, Shandong 271000, China; 'Department of laboratory Medicine. Tai' an Central Hospital, Tai' an, Shandong 271000, China; Department()/ laboratory Medicine Tai' an Cancer Institute, Tai * an , Shandong 271000, China ; 4Department of laboratory Medicine, Second Affiliated Hospital of Shandong First Medical University, Tai' an. Shandong 271000, China Corresponding author： Wang Keqiang, Email: ivkqsd@163. com
第一道防线细胞在黏膜及上皮组织中的高比
例分布，提示7&T细胞在抗微生物、寄生虫及肿瘤 免疫方面起重要作用a： Th 17细胞是a0T细胞的 一个亚型，是•种分泌促炎性细胞因子的CD4 T细 胞亚群，广泛参与慢性炎症、自身免疫性疾病、肿瘤 以及感染性疾病的发生与发展，主要分泌细胞因子
IL-17.ROR71是其关键的核转录因子“叫1L-17是 HT细胞和Thl7细胞的重要效应因子，具有强大的 促炎作用”慢性乙型肝炎(CHB)是一种常见的传 染病.严重危害人民的身体健康，当前各种药物和 治疗方法均不能有效清除已经整合到肝细胞内的 乙型肝炎病毒本研究用流式细胞术(FCM)及 ELISA检测CHB患者外周血中yST细胞、Thl7细胞 的数量及细胞因子1L-17的表达情况，探讨78T细
[Key words] Hepatitis B, chronic; y8T cells; Th 17 cells; Interleukin 17; Cytokines Fund Program: Natural Science Foundation of Shandong Province (ZR2020MH312) ; Shandong Provincial Health Science and Technology Assoc iation Project (2016BJ0003) ; Tai' an Science and Teclinolog\, Innovation Development Project (2018NS01 16 ,2020NS129) DOI ：10. 3760/cma. j. cnl 15668-20210125-00016
162 •
中华生物医学工程杂志2021年4月第27卷第2期 Chin J Biomed Eng, April 2021, Vol. 27, No.2
•新技术与临床•
y5T细胞.Th 17细胞和IL-17在慢性 乙型肝炎患者外周血中表达的意义
晋红梅「王雅姝：苑振新'吴家锋4王克强* 「山东第一医科大学第二附属医院输血科，山东泰安271000；:泰安市中心医院检验
【关键词】乙型肝炎.慢性；2T细胞；Thl7细胞；白细胞介素17；细胞因子类 基金项目：山东省自然科学基金(ZB2O2OMH312)；山东省保健科技协会计划课题(2016BJ0003 )； 泰安市科技创新发展项目(2018NS0116、2020NS129) DOI: 10. 3760/ema. j. cnl 15668-20210125-00016
科，山东泰安271000； 3泰安市肿瘤防治院检验科，山东泰安271000； 4山东第一医科 大学第二附属医院检验科，山东泰安271000 通信作者:王克强，Email: wkqscl@
【摘要】目的了解RT细胞,Thl7细胞和IL-17在慢性乙型肝炎(CHB)患者外周血中的表达情 况，探讨RT细胞、Thl7细胞和IL-17在CHB发病机制中可能的作用：方法随机选取2015年11月至 2016年4月本院门诊或住院收治的慢性无症状HBV携带者(AsC)20例、CHB轻度患者20例、CHB中和 重度患者20例、同期健康体检者20例用流式细胞仪检测外周血中2T细胞和Th 17细胞的数量,用 ELISA法检测各组血清中IL-17细胞因子的含量应用Pearson相关分析4组研究对象外周血中询T、 Thl7细胞的百分率及血清中IL.-17水平三者之间的相关性结果健康人、AsC、CHB轻度患者和CHB 中、重度患者Y$T细胞的表达量分别为(1.258±0.135)%,(2.178±0.195)%,(4.160+0.070)%,(7.058+ 0.970)%，差异具有统计学意义(F=498.35. P=0.000) ；Thl7细胞的表达量分别为(1.252±0.155)%、 (1.714±0.103)%、(2.338±0.234)%、(3.826±0.488)%，差异有统计学意义(F=310.65, P=0.000) ；IL-17 的 浓度分别为(16.307± 19.25) pg/ml、(92.706+16.85) pg/ml、( 147.635±6.32) pg/ml .(391.787+28.52) pg/ml, 差异有统计学意义(F=349.41/=0.00()) Pearson相关性分析显示,CHB患者外周血中沦「细胞和Thl7 细胞的百分率存在正相关(r=0.978, P=().()()0) ; 2T细胞的百分率和II.-I7存在正相关(r=0.959, P= 0.000)；Th 17细胞的百分率和IL-17存在正相关(r=0.954.P=0.000)=结论 2T细胞、Thl7细胞和IL-17 可能参与『机体感染乙型肝炎病毒后引起的免疫反应和组织损伤过程.并与CHB的重症化密切相关