Test Three 091210

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三点测验

三点测验
三点测验
对三对性状差异的两个纯系 亲本进行杂交,得到三杂合 体。在与三隐性体进行测交。
以玉米为例
P: 凹陷 非糯性 有色 × 饱满 糯性 无色 shsh ++ ++ ++ wxwx cc
F1及测交
饱满 非糯性 有色 +sh +wx +c
×
凹陷
Hale Waihona Puke 糯性 无色 ccshsh wxwx
考察测交后代的表现性,进行分类 统计。按各表现型的个体数,对测 交后代进行分组,并进一步确定两 种亲本类型和交换类型。
RF(wx-sh之间)=(601+626+4+2)/6708=18.4% RF(sh-c之间)=(116+113+4+2)/6708=3.5%
RF(wx-c之间)=18.4%+3.5%=21.9%
绘制连锁遗传图:
21.9 18.4 3.5
三个重组值在同一基因型同一环境条件 下得到,可以得到双交换资料
不易得到三杂合体
+ +
c
113 单交换
116 4 双交换 2 6708
wx + + +
sh wx c
用双交换型与亲本型相比较,凡改 变了位置的那个基因一定是处在3 个基因的中央基因。
如:+wxc与sh++相比只有sh位 点不同,因此可以断定sh位 点位于wx和c之间。同理, sh++和+++相比结果也是如此。
计算基因间的交换值:
测交后代的表现性 饱满 糯性 无色 凹陷 非糯 有色 饱满 非糯 无色 凹陷 糯性 有色

OECD 471 bacteria reverse mutation test 9747101E

OECD 471 bacteria reverse mutation test 9747101E

471Adopted:21st July 1997 OECD GUIDELINE FOR TESTING OF CHEMICALSBacterial Reverse Mutation TestINTRODUCTION1.The bacterial reverse mutation test uses amino-acid requiring strains of Salmonella typhimurium and Escherichia coli to detect point mutations, which involve substitution, addition or deletion of one or a few DNA base pairs (1)(2)(3). The principle of this bacterial reverse mutationtest is that it detects mutations which revert mutations present in the test strains and restore the functional capability of the bacteria to synthesize an essential amino acid. The revertant bacteria are detected by their ability to grow in the absence of the amino acid required by the parent test strain.2.Point mutations are the cause of many human genetic diseases and there is substantial evidence that point mutations in oncogenes and tumour suppressor genes of somatic cells are involved in tumour formation in humans and experimental animals. The bacterial reverse mutationtest is rapid, inexpensive and relatively easy to perform. Many of the test strains have several features that make them more sensitive for the detection of mutations, including responsive DNA sequences at the reversion sites, increased cell permeability to large molecules and elimination of DNA repair systems or enhancement of error-prone DNA repair processes. The specificity of the test strains can provide some useful information on the types of mutations that are induced by genotoxic agents. A very large data base of results for a wide variety of structures is available for bacterial reverse mutation tests and well-established methodologies have been developed for testing chemicalswith different physico-chemical properties, including volatile compounds.3.Definitions used are set out in the Annex.INITIAL CONSIDERATIONS4.The bacterial reverse mutation test utilises prokaryotic cells, which differ from mammalian cells in such factors as uptake, metabolism, chromosome structure and DNA repair processes. Tests conducted in vitro generally require the use of an exogenous source of metabolic activation. In vitro metabolic activation systems cannot mimic entirely the mammalian in vivo conditions. The test therefore does not provide direct information on the mutagenic and carcinogenic potency of a substance in mammals.5.The bacterial reverse mutation test is commonly employed as an initial screen for genotoxic activity and, in particular, for point mutation-inducing activity. An extensive data base has demonstrated that many chemicals that are positive in this test also exhibit mutagenic activity in other tests. There are examples of mutagenic agents which are not detected by this test; reasons for these shortcomings can be ascribed to the specific nature of the endpoint detected, differences in metabolic activation, or differences in bioavailability. On the other hand, factors which enhance the sensitivity of the bacterial reverse mutation test can lead to an overestimation of mutagenic activity.471OECD/OCDE6.The bacterial reverse mutation test may not be appropriate for the evaluation of certainclasses of chemicals, for example highly bactericidal compounds (e.g. certain antibiotics) and those which are thought (or known) to interfere specifically with the mammalian cell replication system(e.g. some topoisomerase inhibitors and some nucleoside analogues). In such cases, mammalianmutation tests may be more appropriate.7.Although many compounds that are positive in this test are mammalian carcinogens, thecorrelation is not absolute. It is dependent on chemical class and there are carcinogens that are not detected by this test because they act through other, non-genotoxic mechanisms or mechanisms absent in bacterial cells.PRINCIPLE OF THE TEST METHOD8.Suspensions of bacterial cells are exposed to the test substance in the presence and in theabsence of an exogenous metabolic activation system. In the plate incorporation method, these suspensions are mixed with an overlay agar and plated immediately onto minimal medium. In the preincubation method, the treatment mixture is incubated and then mixed with an overlay agar before plating onto minimal medium. For both techniques, after two or three days of incubation, revertant colonies are counted and compared to the number of spontaneous revertant colonies on solvent control plates.9.Several procedures for performing the bacterial reverse mutation test have been described.Among those commonly used are the plate incorporation method (1)(2)(3)(4), the preincubation method (2)(3)(5)(6)(7)(8), the fluctuation method (9)(10), and the suspension method (11).Modifications for the testing of gases or vapours have been described (12).10.The procedures described in this guideline pertain primarily to the plate incorporation andpreincubation method s. Either of them is acceptable for conducting experiments both with and without metabolic activation. Some compounds may be detected more efficiently using the preincubation method. These compounds belong to chemical classes that include short chain aliphatic nitrosamines, divalent metals, aldehydes, azo-dyes and diazo compounds, pyrollizidine alkaloids, allyl compounds and nitro compounds (3). It is also recognised that certain classes of mutagens are not always detected using standard procedures such as the plate incorporation method or preincubation method. These should be regarded as "special cases" and it is strongly recommended that alternative procedures should be used for their detection. The following "special cases" could be identified (together with examples of procedures that could be used for their detection): azo-dyes and diazo compounds (3)(5)(6)(13), gases and volatile chemicals(12)(14)(15)(16), and glycosides (17)(18). A deviation from the standard procedure needs to bescientifically justified.DESCRIPTION OF THE METHODPreparationsBacteria11.Fresh cultures of bacteria should be grown up to the late exponential or early stationaryphase of growth (approximately 109 cells per ml). Cultures in late stationary phase should not be used. It is essential that the cultures used in the experiment contain a high titre of viable bacteria.The titre may be demonstrated either from historical control data on growth curves, or in each assay through the determination of viable cell numbers by a plating experiment.OECD/OCDE47112.The recommended culture temperature is 37°C.13.At least five strains of bacteria should be used. These should include four strains of S. typhimurium (TA1535; TA1537 or TA97a or TA97; TA98; and TA100) that have been shown to be reliable and reproducibly responsive between laboratories. These four S. typhimurium strains haveGC base pairs at the primary reversion site and it is known that they may not detect certain oxidising mutagens, cross-linking agents and hydrazines. Such substances may be detected by E.coli WP2 strains or S. typhimurium TA102 (19) which have an AT base pair at the primary reversion site. Therefore the recommended combination of strains is:1.S. typhimurium TA1535, and2.S. typhimurium TA1537 or TA97 or TA97a, and3.S. typhimurium TA98, and4.S. typhimurium TA100, and5. E. coli WP2 uvrA, or E. coli WP2 uvrA (pKM101), or S. typhimurium TA102.In order to detect cross-linking mutagens it may be preferable to include TA102 or to add a DNA repair-proficient strain of E.coli [e.g. E.coli WP2 or E.coli WP2 (pKM101).]14.Established procedures for stock culture preparation, marker verification and storage shouldbe used. The amino-acid requirement for growth should be demonstrated for each frozen stock culture preparation (histidine for S. typhimurium strains, and tryptophan for E. coli strains). Other phenotypic characteristics should be similarly checked, namely: the presence or absence of R-factor plasmids where appropriate [i.e. ampicillin resistance in strains TA98, TA100 and TA97a or TA97,WP2 uvrA and WP2 uvrA (pKM101), and ampicillin + tetracycline resistance in strain TA102]; the presence of characteristic mutations (i.e. rfa mutation in S. typhimurium through sensitivity to crystal violet, and uvrA mutation in E. coli or uvrB mutation in S. typhimurium, through sensitivity to ultra-violet light) (2)(3). The strains should also yield spontaneous revertant colony plate counts withinthe frequency ranges expected from the laboratory's historical control data and preferably within the range reported in the literature.Medium15.An appropriate minimal agar (e.g. containing Vogel-Bonner minimal medium E and glucose) and an overlay agar containing histidine and biotin or tryptophan, to allow for a few cell divisions, is used (1)(2)(9).Metabolic activation16.Bacteria should be exposed to the test substance both in the presence and absence of an appropriate metabolic activation system. The most commonly used system is a cofactor-supplemented post-mitochondrial fraction (S9) prepared from the livers of rodents treated with enzyme-inducing agents such as Aroclor 1254 (1)(2) or a combination of phenobarbitone and ß-naphthoflavone (18)(20)(21). The post-mitochondrial fraction is usually used at concentrations in the range from 5 to 30% v/v in the S9-mix. The choice and condition of a metabolic activation systemmay depend upon the class of chemical being tested. In some cases it may be appropriate to utilizemore than one concentration of post-mitochondrial fraction. For azo-dyes and diazo-compounds,using a reductive metabolic activation system may be more appropriate (6)(13).471OECD/OCDETest substance/Preparation17.Solid test substances should be dissolved or suspended in appropriate solvents or vehiclesand diluted if appropriate prior to treatment of the bacteria. Liquid test substances may be added directly to the test systems and/or diluted prior to treatment. Fresh preparations should be employed unless stability data demonstrate the acceptability of storage.Test conditionsSolvent/vehicle18.The solvent/vehicle should not be suspected of chemical reaction with the test substanceand should be compatible with the survival of the bacteria and the S9 activity (22). If other than well-known solvent/vehicles are used, their inclusion should be supported by data indicating their compatibility. It is recommended that wherever possible, the use of an aqueous solvent/vehicle be considered first. When testing water-unstable substances, the organic solvents used should be free of water.Exposure concentrations19.Amongst the criteria to be taken into consideration when determining the highest amount oftest substance to be used are cytotoxicity and solubility in the final treatment mixture. It may be useful to determine toxicity and insolubility in a preliminary experiment. Cytotoxicity may be detected by a reduction in the number of revertant colonies, a clearing or diminution of the background lawn, or the degree of survival of treated cultures. The cytotoxicity of a substance may be altered in the presence of metabolic activation systems. Insolubility should be assessed as precipitation in the final mixture under the actual test conditions and evident to the unaided eye. The recommended maximum test concentration for soluble non-cytotoxic substances is 5 mg/plate or5 µl/plate. For non-cytotoxic substances that are not soluble at 5 mg/plate or 5 µl/plate, one or moreconcentrations tested should be insoluble in the final treatment mixture. Test substances that are cytotoxic already below 5 mg/plate or 5 µl/plate should be tested up to a cytotoxic concentration.The precipitate should not interfere with the scoring.20.At least five different analysable concentrations of the test substance should be used withapproximately half log (i.e. √10) intervals between test points for an initial experiment. Smaller intervals may be appropriate when a concentration-response is being investigated.21.Testing above the concentration of 5 mg/plate or 5 µl/plate may be considered whenevaluating substances containing substantial amounts of potentially mutagenic impurities.Controls22.Concurrent strain-specific positive and negative (solvent or vehicle) controls, both with andwithout metabolic activation, should be included in each assay. Positive control concentrations that demonstrate the effective performance of each assay should be selected.23.For assays employing a metabolic activation system, the positive control referencesubstance(s) should be selected on the basis of the type of bacteria strains used. The following chemicals are examples of suitable positive controls for assays with metabolic activation:OECD/OCDE471Chemical and CAS No.9,10-Dimethylanthracene [CAS no. 781-43-1]7,12-Dimethylbenzanthracene [CAS no. 57-97-6]Congo Red [CAS no. 573-58-0] (for the reductive metabolic activation method)Benzo(a)pyrene [CAS no. 50-32-8]Cyclophosphamide (monohydrate) [CAS no. 50-18-0 (CAS no. 6055-19-2)]2-Aminoanthracene [CAS no. 613-13-8]2-Aminoanthracene should not be used as the sole indicator of the efficacy of the S9-mix. If 2-aminoanthracene is used, each batch of S9 should also be characterised with a mutagen that requires metabolic activation by microsomal enzymes, e.g., benzo(a)pyrene, dimethylbenzanthracene.24.For assays performed without metabolic activation system, examples of strain-specific positive controls are:Chemical and CAS No.Strain(a)Sodium azide [CAS no. 26628-22-8]TA1535 and TA100(b)2-Nitrofluorene [CAS no. 607-57-8]TA98(c)9-Aminoacridine [CAS no. 90-45-9]or ICR191 [CAS no. 17070-45-0]TA1537, TA97 and TA97a(d)Cumene hydroperoxide [CAS no. 80-15-9]TA102(e)Mitomycin C [CAS no. 50-07-7]WP2 uvrA and TA102(f)N-Ethyl-N-nitro-N-nitrosoguanidine [CAS no. 70-25-7] or4-nitroquinoline 1-oxide [CAS no. 56-57-5]WP2, WP2 uvrA and WP2 uvrA (pKM101)(g)Furylfuramide (AF-2) [CAS no. 3688-53-7]plasmid-containing strains25.Other appropriate positive control reference substances may be used. The use of chemical class-related positive control chemicals may be considered, when available.26.Negative controls, consisting of solvent or vehicle alone, without test substance, and otherwise treated in the same way as the treatment groups, should be included. In addition, untreated controls should also be used unless there are historical control data demonstrating that no deleterious or mutagenic effects are induced by the chosen solvent.471OECD/OCDEPROCEDURETreatment with test substance27.For the plate incorporation method (1)(2)(3)(4), without metabolic activation, usually 0.05ml or 0.1 ml of the test solutions, 0.1 ml of fresh bacterial culture (containing approximately 108 viable cells) and 0.5 ml of sterile buffer are mixed with 2.0 ml of overlay agar. For the assay with metabolic activation, usually 0.5 ml of metabolic activation mixture containing an adequate amount of post-mitochondrial fraction (in the range from 5 to 30% v/v in the metabolic activation mixture) are mixed with the overlay agar (2.0 ml), together with the bacteria and test substance/test solution.The contents of each tube are mixed and poured over the surface of a minimal agar plate. The overlay agar is allowed to solidify before incubation.28.For the preincubation method (2)(3)(5)(6) the test substance/test solution is preincubatedwith the test strain (containing approximately 108 viable cells) and sterile buffer or the metabolic activation system (0.5 ml) usually for 20 min. or more at 30°-37°C prior to mixing with the overlay agar and pouring onto the surface of a minimal agar plate. Usually, 0.05 or 0.1 ml of test substance/test solution, 0.1 ml of bacteria, and 0.5 ml of S9-mix or sterile buffer, are mixed with 2.0 ml of overlay agar. Tubes should be aerated during pre-incubation by using a shaker.29.For an adequate estimate of variation, triplicate plating should be used at each dose level.The use of duplicate plating is acceptable when scientifically justified. The occasional loss of a plate does not necessarily invalidate the assay.30.Gaseous or volatile substances should be tested by appropriate methods, such as in sealedvessels (12)(14)(15)(16).Incubation31.All plates in a given assay should be incubated at 37°C for 48-72 hours. After theincubation period, the number of revertant colonies per plate is counted.DATA AND REPORTINGTreatment of results32.Data should be presented as the number of revertant colonies per plate. The number ofrevertant colonies on both negative (solvent control, and untreated control if used) and positive control plates should also be given.33.Individual plate counts, the mean number of revertant colonies per plate and the standarddeviation should be presented for the test substance and positive and negative (untreated and/or solvent) controls.34.There is no requirement for verification of a clear positive response. Equivocal resultsshould be clarified by further testing preferably using a modification of experimental conditions.Negative results need to be confirmed on a case-by-case basis. In those cases where confirmation of negative results is not considered necessary, justification should be provided. Modification of study parameters to extend the range of conditions assessed should be considered in follow-up experiments.Study parameters that might be modified include the concentration spacing, the method of treatment (plate incorporation or liquid preincubation), and metabolic activation conditions.OECD/OCDE471 Evaluation and interpretation of results35.There are several criteria for determining a positive result, such as a concentration-related increase over the range tested and/or a reproducible increase at one or more concentrations in the number of revertant colonies per plate in at least one strain with or without metabolic activation system (23). Biological relevance of the results should be considered first. Statistical methods maybe used as an aid in evaluating the test results (24). However, statistical significance should not bethe only determining factor for a positive response.36. A test substance for which the results do not meet the above criteria is considered non-mutagenic in this test37.Although most experiments will give clearly positive or negative results, in rare cases thedata set will preclude making a definite judgement about the activity of the test substance. Resultsmay remain equivocal or questionable regardless of the number of times the experiment is repeated.38.Positive results from the bacterial reverse mutation test indicate that a substance inducespoint mutations by base substitutions or frameshifts in the genome of either Salmonella typhimuriumand/or Escherichia coli. Negative results indicate that under the test conditions, the test substance isnot mutagenic in the tested species.Test report39.The test report must include the following information:Test substance:-identification data and CAS no., if known;-physical nature and purity;-physicochemical properties relevant to the conduct of the study;-stability of the test substance, if known.Solvent/Vehicle:-justification for choice of solvent/vehicle;-solubility and stability of the test substance in solvent/vehicle, if known.Strains:-strains used;-number of cells per culture;-strain characteristics.Test conditions:-amount of test substance per plate (mg/plate or µg/plate) with rationale for selection of dose and number of plates per concentration;-media used;-type and composition of metabolic activation system, including acceptability criteria;-treatment procedures.471OECD/OCDEResults:-signs of toxicity;-signs of precipitation;-individual plate counts;-the mean number of revertant colonies per plate and standard deviation;-dose-response relationship, where possible;-statistical analyses, if any;-concurrent negative (solvent/vehicle) and positive control data, with ranges, means and standard deviations;-historical negative (solvent/vehicle) and positive control data, with e.g. ranges, means and standard deviations.Discussion of the results.Conclusion.LITERATURE(1)Ames, B.N., McCann, J. and Yamasaki, E. (1975). Methods for Detecting Carcinogens andMutagens with the Salmonella/Mammalian-Microsome Mutagenicity Test. Mutation Res., 31,347-364.(2)Maron, D.M. and Ames, B.N. (1983). Revised Methods for the Salmonella Mutagenicity Test.Mutation Res., 113, 173-215.(3)Gatehouse, D., Haworth, S., Cebula, T., Gocke, E., Kier, L., Matsushima, T., Melcion, C.,Nohmi, T., Venitt, S. and Zeiger, E. (1994). Recommendations for the Performance ofBacterial Mutation Assays. Mutation Res., 312, 217-233.(4)Kier, L.D., Brusick D.J., Auletta, A.E., Von Halle, E.S., Brown, M.M., Simmon, V.F., Dunkel,V., McCann, J., Mortelmans, K., Prival, M., Rao, T.K. and Ray V. (1986). The SalmonellaTyphimurium/Mammalian Microsomal Assay: A Report of the U.S. Environmental ProtectionAgency Gene-tox Program. Mutation Res., 168, 69-240.(5)Yahagi, T., Degawa, M., Seino, Y.Y., Matsushima, T., Nagao, M., Sugimura, T. andHashimoto, Y. (1975). Mutagenicity of Carcinogen Azo Dyes and their Derivatives. CancerLetters, 1, 91-96.(6)Matsushima, M., Sugimura, T., Nagao, M., Yahagi, T., Shirai, A., and Sawamura, M. (1980).Factors Modulating Mutagenicity Microbial Tests. In: Short-term Test Systems for DetectingCarcinogens. Ed. Norpoth K.H. and Garner, R.C., Springer, Berlin-Heidelberg-New York. pp.273-285.(7)Gatehouse, D.G., Rowland, I.R., Wilcox, P., Callender, R.D. and Foster, R. (1990). BacterialMutation Assays. In: Basic Mutagenicity Tests: UKEMS Part 1 Revised. Ed. D.J. KirklandCambridge University Press, pp. 13-61.(8)Aeschbacher, H.U., Wolleb, U. and Porchet, L. (1987). Liquid Preincubation Mutagenicity Testfor Foods. J. Food Safety, 8, 167-177.OECD/OCDE471 (9)Green, M. H. L., Muriel, W. J. and Bridges, B.A. (1976). Use of a simplified fluctuation test todetect low levels of mutagens. Mutation Res., 38, 33-42.(10)Hubbard, S.A., Green, M.H.L., Gatehouse, D., and J.W. Bridges (1984). The Fluctuation Test inBacteria. In: Handbook of Mutagenicity Test Procedures. 2nd Edition. Ed. Kilbey, B.J.,Legator , M.,Nichols, W. and Ramel C., Elsevier, Amsterdam-New York-Oxford,pp. 141-161. (11)Thompson, E.D. and Melampy, P.J. (1981). An Examination of the Quantitative SuspensionAssay for Mutagenesis with Strains of Salmonella typhimurium. Environmental Mutagenesis,3, 453-465.(12)Araki, A., Noguchi, T., Kato, F. and T. Matsushima (1994). Improved Method forMutagenicity Testing of Gaseous Compounds by Using a Gas Sampling Bag. Mutation Res.,307, 335-344.(13)Prival, M.J., Bell, S.J., Mitchell, V.D., Reipert, M.D. and Vaughn, V.L. (1984). Mutagenicityof Benzidine and Benzidine-Congener Dyes and Selected Monoazo Dyes in a ModifiedSalmonella Assay. Mutation Res., 136, 33-47.(14)Zeiger, E., Anderson, B. E., Haworth, S, Lawlor, T. and Mortelmans, K. (1992). SalmonellaMutagenicity Tests. V. Results from the Testing of 311 Chemicals. Environ. Mol. Mutagen., 19,2-141.(15)Simmon, V., Kauhanen, K. and Tardiff, R.G. (1977). Mutagenic Activity of ChemicalsIdentified in Drinking Water. In Progress in Genetic Toxicology, D. Scott, B. Bridges and F.Sobels (Eds.)., Elsevier, Amsterdam, pp. 249-258.(16)Hughes, T.J., Simmons, D.M., Monteith, I.G. and Claxton, L.D. (1987). VaporizationTechnique to Measure Mutagenic Activity of Volatile Organic Chemicals in theAmes/Salmonella Assay. Environmental Mutagenesis, 9, 421-441.(17)Matsushima, T., Matsumoto, A., Shirai, M., Sawamura, M. and Sugimura, T. (1979).Mutagenicity of the Naturally Occurring Carcinogen Cycasin and Synthetic MethylazoxyMethane Conjugates in Salmonella typhimurium. Cancer Res., 39, 3780-3782.(18)Tamura, G., Gold, C., Ferro-Luzzi, A. and Ames. B.N. (1980). Fecalase: A Model forActivation of Dietary Glycosides to Mutagens by Intestinal Flora. Proc. Natl. Acad. Sci. USA,77, 4961-4965.(19)Wilcox, P., Naidoo, A., Wedd, D. J. and Gatehouse, D. G. (1990). Comparison of Salmonellatyphimurium TA 102 with Escherichia coli WP2 Tester strains. Mutagenesis, 5, 285-291.(20)Matsushima, T., Sawamura, M., Hara, K. and Sugimura, T. (1976). A Safe Substitute forPolychlorinated Biphenyls as an Inducer of Metabolic Activation Systems. In: "In vitroMetabolic Activation in Mutagenesis Testing", Eds F.J. de Serres et al. Elsevier, North Holland,pp. 85-88.(21)Elliott, B.M., Combes, R.D., Elcombe, C.R., Gatehouse, D.G., Gibson, G.G., Mackay, J.M. andWolf, R.C. (1992). Alternatives to Aroclor 1254-induced S9 in in vitro Genotoxicity Assays.Mutagenesis, 7, 175-177.(22)Maron, D., Katzenellenbogen, J., and Ames, B.N. (1981). Compatibility of Organic Solventswith the Salmonella/Microsome Test. Mutation Res., 88, 343-350.471OECD/OCDE(23)Claxton, L.D., Allen, J., Auletta, A., Mortelmans, K., Nestmann, E., and Zeiger, E., (1987).Guide for the Salmonella typhimurium/Mammalian Microsome Tests for BacterialMutagenicity. Mutation Res., 189, 83-91.(24)Mahon, G.A.T., Green, M.H.L., Middleton, B., Mitchell, I., Robinson, W.D. and Tweats, D.J.(1989). Analysis of Data from Microbial Colony Assays. In: UKEMS Sub-Committee onGuidelines for Mutagenicity Testing Part II. Statistical Evaluation of Mutagenicity Test Data.Ed. Kirkland, D.J., Cambridge University Press, pp. 28-65.OECD/OCDE471ANNEXDEFINITIONSA reverse mutation test in either Salmonella typhimurium or Escherichia coli detects mutation in an amino-acid requiring strain (histidine or tryptophan, respectively) to produce a strain independent of an outside supply of amino-acid.Base pair substitution mutagens are agents that cause a base change in DNA. In a reversion test this change may occur at the site of the original mutation, or at a second site in the bacterial genome.Frameshift mutagens are agents that cause the addition or deletion of one or more base pairs in the DNA, thus changing the reading frame in the RNA11/11。

BlueTest3 使用向导

BlueTest3 使用向导

5 Chip and Firmware Support for Tests ........................................................................................................... 11
6 Simple Tests ................................................................................................................................................. 12
27 SEP 10
Updates to RXSTART1, RXSTART2, RXDATA1, RXDATA2, BIT ERR1 and BIT ERR2.
14 FEB 11
Descriptions added for new routines BLE TEST TX, BLE TEST RX and BLE TEST
12 6.5 RXSTART1 .......................................................................................................................................... 14 20 6.6 RXSTART2 .......................................................................................................................................... 14 6, Quantitative Tests ......................................................................................................................................... 15 r 1 7.1 Transmission Tests ..................................................................................................................._äìÉ`çêÉ BlueTest3 User Guide

the penn emotion recognition test 2001

the penn emotion recognition test 2001

the penn emotion recognition test 2001 The Penn Emotion Recognition Test (PERT) is a widely used and validated tool for assessing an individual's ability to recognize facial expressions of different emotions. Developed in 2001, this test has become an important tool in psychological research and clinical practices.The PERT consists of a series of photographs or videos displaying faces expressing various emotions such as happiness, sadness, anger, fear, and surprise. Participants are asked to identify the emotion portrayed in each facial expression. The test measures an individual's accuracy in recognizing and labeling these emotions.The primary goal of the PERT is to assess an individual's ability to accurately interpret emotional expressions, which plays a crucial role in social interactions and understanding others' feelings. People who struggle with recognizing emotions may experience difficulties in interpersonal relationships and various aspects of their lives, affecting their overall well-being.To administer the PERT, researchers or clinicians present a set of stimuli, either through photographs or videos, to the participants.These stimuli contain a range of facial expressions, and the participants are required to identify the emotion displayed. Each response is then scored for accuracy, allowing for a quantitative assessment of the individual's ability to recognize emotions.The PERT has been widely studied and has shown good reliability and validity across different populations and cultural backgrounds. The test has been used in various research fields, including psychology, neuroscience, and social sciences, to understand the underlying mechanisms of emotion recognition and its impact on human behavior.One major advantage of the PERT is its ability to provide quantitative data on emotion recognition abilities. By scoring responses for accuracy, researchers can identify specific areas of strengths and weaknesses in individuals' emotion recognition skills. This information can be helpful in diagnosing and creating tailored interventions for individuals with emotion recognition difficulties.Additionally, the PERT has been used in research to investigate the influence of various factors on emotion recognition abilities. For example, studies have explored the impact of age, gender, culturalbackground, and neurological conditions on individuals' ability to recognize emotions accurately. These findings have contributed to a better understanding of the differences in emotion recognition across populations.Despite its strengths, the PERT also has limitations. This test primarily focuses on only facial expressions, neglecting other important cues such as body language and vocal tone, which also play a role in recognizing emotions accurately. Additionally, the test results may be influenced by participants' prior experiences and cultural backgrounds, as the interpretation of emotions can vary across cultures.In conclusion, the Penn Emotion Recognition Test (PERT) has proven to be a valuable tool in assessing an individual's ability to recognize facial expressions of emotions accurately. It provides researchers and clinicians with quantitative data on emotion recognition abilities, allowing for targeted interventions and furthering our understanding of this important aspect of human behavior. While the PERT has its limitations, it remains a valuableand widely used tool in the field of emotion research.。

test3

test3

READINGREADING PASSAGE 1You should spend about 20 minutes on Questions 1-13, which are based on Reading Passage 1 on the following pages.Questions 1-4Reading Passage 1 has five paragraphs, A-E.Choose the correct heading for paragraphs B-E from the list of headings below. White the correct number, i-vii, in boxes 1-4 on your answer sheet.List of headingsi Economic and social significance of tourismii The development of mass tourismiii Travel for the wealthyiv Earning foreign exchange through tourismv Difficulty in recognising the economic effects of tourismvi The contribution of air travel to tourismvii The world impact of tourismviii The history of travelExample AnswerParagraph A viii1Paragraph A2Paragraph B3Paragraph C4Paragraph D5Paragraph EThe Context, Meaning and Scope of TourismA Travel has existed since the beginning of time, when primitive man set out , often traversing great distances in search of games, which provided the food and clothing necessary for his survival. Throughout the course of history, people have travelled for purposes of trade, religious conviction, economic gain, war, migration and other equally compelling motivations. In the Roman era, wealthy aristocrats and high government officials also travelled for pleasure. Seaside resorts located at Pompeii and Herculaneum afforded citizens the opportunity to escape to their vacation villas in order to avoid the summer heat of Rome .Travel, except during the Dark Ages,has continued to grow and, throughout recorded history, has played a vital role in the development of civilisations and their economies.B Tourism in the mass from as we know it today is a distinctly twentieth-century phenomenon. Historians suggest that the advent of mass tourism began in England during the industrial revolution with the rise of the middle class and the availability of relatively inexpensive transportation. The creation of the commercial airline industry following the Second World War and the subsequent development of the jet aircraft in the 1950s signalled the rapid growth and expansion of international travel. This growth led to the development of a major new industry: tourism. In turn, international tourism became the concern of a number of world governments since it not provided new employment opportunities but also produced a means of earning foreign exchange.C Tourism today has grown significantly in both economic and social importance.In most industrialised countries over the past few years the fasted growth has been seen in the area of services. One of the largest segments of the service industry, although largely unrecognised as an entity in some of these countries, is travel and tourism. According to the World Travel and Tourism Council (1992), ‘Travel and tourism is the largest industry in the world on virtually any economic measure including value-added capital investment, employment and tax contributions’. In 1992, the industry’s gross output was estimated to be $3.5 trillion, over 12 per cent of all consumer spending. The travel and tourism industry is the world’s largest employer with almost 130 million jobs, or almost 7 per cent of all employees. This industry is the world's leading industrial contributor, producing over 6 per cent of the world's gross national product and accounting for capital investment in excess of $422 billionin direct, indirect and personal taxes each year. Thus, tourism has a profound impact both on the world economy and, because of the educative effect of travel and the effects on employment, on society itself.D However, the major problems of the travel and tourism industry that have hidden, or obscured, its economic impact are the diversity and fragmentation of the industry itself. The travel industry includes: hotels, motels and other types of accommodation; restaurants and other food services; transportation services and facilities; amusements, attractions and other leisure facilities; gift shops and a large number of other enterprises. Since many of these businesses also serve local residents, the impact of spending by visitors can easily be overlooked or underestimated. In addition, Meis (1992) points out that the tourism industry involves concepts that have remained amorphous to both analysis and decision makers. Moreover, in all nations this problem has made difficult tor the industry to develop any type of reliable or credible tourism information base in order to estimate the contribution it makes to regional, national and global economics. However, the nature of this very diversity makes travel and tourism ideal vehicles for economic development in a wide variety of couniries, regions or communitiesE Once the exclusive province of the wealthy, travel and tourism have become an institutionalised way of life for most of the population. In fact,McIntosh and GoeIdner (I990) suggest that tourism has become the largest commodity in international trade for many nations and, for a significant number of other countries, it ranks second or third. For example, tourism is the major source of income in Bermuda, Greece, Italy, Spain, Switzerland and most Caribbean countries. In addition. Hawkins and Ritchie, quoting from data published by the American Express Company, suggest that the travel and tourism industry is the number one ranked employer in the Bahamas. Brazil, Canada, France, (the former) West Germany Hong Kong, Italy, Jamaica, Japan, Singapore, the United Kingdom and the United States. However, because of problems of definition, which directly affect statistical measurement, itis not Possible with any degree of certainty to provide precise, valid or reliable data about the extent of world-wide tourism participation or its economic impact. In many case, similar difficulties arise when attempts are made to measure domestic tourism.Questions 5- 10Do the following statements agree with the information given in Reading Passage 1? In boxes 5-10 on your answer sheet, writeTRUE if the statement agrees with the informationFALSE if the statement agrees with the informationNOT GIVEN if the statement agrees with the information5The largest employment figures in the world are found in the travel and tourism industry.6 Tourism contributes over six per cent of the Australian gross national product.7Tourism has a social impact because it promotes recreation.8 Two main features of the travel and tourism industry make its economic significance difficultto ascertain.9Visitor spending is always greater than the spending of residents in tourist areas. 10It is easy to show statistically how tourism affects individual economies. Question 11-13Complete the sentences below.Choose NO MORE THAN THREE WORD from the passage for each answer. Write your answer answers in boxes 6-8 on your answer sheet.11 In Greece, tourism is the most important ______.12 The travel and tourism industry in Jamaica is the major______.13 The problems associated with measuring international tourism are often reflected inthe measurement of ______.READING PASSAGE 2You should spend about 20 minutes on Questions 14-26, which are based on Reading Passage 2 below.Autumn leavesCanadian writer Jay Ingram investigates the mystery ofwhy leaves turn red in the fallA One of the most captivating natural events of the year in many areas throughout North America is the turning of the leaves in the fall. The colours are magnificent, but the question of exactly why some trees turn yellow or orange, and others red or purple, is something which has long puzzled scientists.B Summer leaves are green because they are full of chlorophyll, the molecule that captures sunlight and converts that energy into new building materials for the tree. As fall approaches in the northern hemisphere, the amount of solar energy available declines considerably. For many trees - evergreen conifers being an exception - the best strategy is to abandon photosynthesis* until the spring. So rather than maintaining the now redundant leaves throughout the winter, the tree saves its precious resources and discards them. But before letting its leaves go, the tree dismantles their chlorophyll is depleted. This unmasking explains the autumn colours of yellow and orange, but not be brilliant reds and purple of trees such as the maple or sumac.C The source of the red is widely known: it is created by anthocyanins, water-soluble plant pigments reflecting the red to blue range of the visible spectrum. They belong to a class of sugar-based chemical compounds also known as flavonoids. What’s puzzling is to anthocyanins are actually newly minted, made in the leaves at the same lime as the tree is preparing to drop them. But it is hard to make sense of the manufacture of anthocyanins - why should a tree bother making new chemicals in its leaves when it’s already scrambling to withdraw and preserve the ones already there?D Some theories about anthocyanins have argued that they might act as a chemical defence against attacks by insect or fungi, or that they might attract fruit-eating birds or increase a leafs tolerance to freezing. However there are problems with each of these theories, including the fact that leaves are red for such a relatively short period that the expense of energy needed to manufacture the anthocyanins would outweigh any anti-fungal or anti-herbivore activity achieved.E It has also been proposed that trees may produce vivid red colours to convinceherbivorous insects that they are healthy and robust and would be easily able to mount chemical defences against infestation. If insects paid attention to such advertisements, they might be promoted to lay their eggs on a duller, and presumably less resistant host. The flaw in this theory lies in the lack proof the support it. No one has as yet ascertained whether more robust trees sport the brightest leaves, or whether insects make choices according to colour intensity.F Perhaps the most plausible suggestion as to why leaves would go to the trouble of making anthocyanins when they’re busy packing up for the winter is the theory known as the ‘light screen’ hypothesis. It sounds paradoxical, because the idea behind hypothesis is that the red pigment is made in autumn leaves to protect chlorophyll, the light-absorbing chemical, from too much light. Why does chlorophyll need protection when it is the natural word’s supreme light absorber? Why protect chlorophyll at a time when the tree is breaking it down to salavage as much of it as possible?G Chlorophyll, although exquisitely evolved to capture the energy of sunlight, can sometimes be overwhelmed by it, especially in situations of drought, low temperatures, or nutrient deficiency. Moreover, the problem of oversensitivity to light is even more acute in the fall, when the leaf is busy preparing for winter by dismantling its internal machinery. The energy absorbed by the chlorophyll molecules of the unstable autumn leaf is not immediately channelled into useful products and processes, as it would be in an intact summer leaf. The weakened fall leaf then becomes vulnerable to the highly destructive effects of the oxygen created by the excited chlorophyll molecules.H Even if you had never suspected that this is what was going on when leaves turn red, there are clues out there. One is straightforward: on many trees, the leaves that are the reddest are those on the side of the tree which gels most sun. Not only that, but the red is brighter on the upper side of the leaf. It has also been recognised for decades that the best conditions for intense red colours are dry, sunny days and cool nights, conditions that nicely match those that make leaves susceptible to excess light. And finally, trees such as maples usually get much redder the more north you travel in the northern hemisphere. It’s colder there, they’re more stressed, their chlorophyll is more sensitive and it needs more sunblock.I What is still not fully understood, however, is why some tr^s resort to producing red pigments while others don’t bother, and simply reveal their orange or yellow hues. Do these trees have other means at their display disposal to prevent overexposure to light in autumn? Their story, though not as spectacular to the eye, will surely turn out to be as subtle and as complex.* photosynthesis: the production of new material from sunlight, water and carbonQuestion 14-18Reading Passage 2 has nine passage, A-I.Which paragraph contains the following information?White the correct number, A-I, in boxes 14-18 on your answer sheet.NB You may use any letter more than once.14 a description of the substance responsible for the red colouration of leaves15the reason why trees drop their leaves in autumn16some evidence to confirm a theory about the purpose of the red leaves17an explanation of the function of chlorophyll18 a suggestion that the red colouration in leaves could serve as a warning signal Questions 19-22Complete the table below.Choose ONE WORD ONLY from the passage for each answer.Write your answer in boxes 19-22 on your answer sheet.Why believe the ‘light screen’ hypothesis?·The most vividly coloured red leaves are found on the side of the tree facing the 19______.·The 20______ surfaces of leaves contain the most red pigment.·Red leaves are most abundant when daytime weather conditions are 21______and sunny.·The intensity of the red colour of leaves increases as you go further 22______. Question 23-25Do the following states agree with the information given in Reading Passage 2?In boxes 23-25 on your answer sheet, writeTRUE if the statement agrees with the informationFALSE if the statement agrees with the informationNOT GIVEN if the statement agrees with the information23It is likely that the red pigments help to protect the leaf from freezing temperatures.24The 'light screen' hypothesis would initially seem to contradict what is known aboutchlorophyll.25Leaves which turn colours other than red are more likely to be damaged by sunlight.Question 26Choose the correct letter A, B, C or D.Write the correct letter in box 26 on your answer sheet. For which of the following questions does the writer offer an explanation?A why c onifers remain green in winterB how l eaves turn orange and yellow in autumnC how h erbivorous insects choose which trees to lay their eggs inD why anthocyanins are restricted to certain treeREADING PASSAGE 3You should spend about 20 minutes on Questions 27-40, which are based on Reading Passage 3 below.Beyond the blue horizonAncient voyagers who settled the far-flung islands of the Pacific Ocean An important archaeological discovery on the island i n of Efare in the Pacific archipelago of Vanuatu has revealed traces of an ancient seafaring people, the distant ancestors of today’s Polynesians. The site came to light only by chance. An agricultural worker, digging in the grounds of a derelict plantation, scraped open a grave - the first of dozens in a burial ground some 3,000 years old. It is the oldest cemetery ever found in the Pacific islands, and it harbors the remains of an ancient people archaeologists call the Lapita.They were daring blue-water adventurers who used basic canoes to rove across the ocean. But they were not just explorers. They were also pioneers who carried with them everything they would need to build new lives - their livestock, taro seedlings and stone tools. Within the span of several centuries, the Lapita stretched the boundaries of their world from the jungle-dad volcanoes of Papua New Guinea to the loneliest coral outliers of Tonga.The Lapita left precious few clues about themselves, but Éfatéexpands the volume of data available to researchers dramatically. The remains of 62 individuals have been uncovered so far, and archaeologists were also thrilled to find six complete Lapita pots. Other items included a Lapita burial urn with modeled birds arranged on the rim as though peering down at the human remains scaled inside. ‘It’s an important discovciy,’says Matthew Spriggs, professor of archaeology at Australian National University and head of the international team digging up the site, ‘for it conclusivelyidentifies the remains Lapita.’DNA teased from these human remains may help answer one of the most puzzling questions inPacific anthropology; did all Pacific islands spring from one dource or many? Was there only one outward migration from a single point in Asia, or several from different points? “This represents the best opportunity we’ve had yet,” says Spriggs, ‘to find out who the Laita actually were, where they came from, and who their closet descendants are today.”There is one stubborn question for which archaeology has yet to provide any answers: how did the Lapita accomplish the ancient equivalent of a moon landing, many times over? No-one has found one of their canoes or any rigging, which could reveal how the canoes were sailed. Nor do the oral histories and traditions of later Polynesians offer any insights, for they turn into myths long before they reach as far back in time as the Lapita.‘All we can say for certain is that the Lapita had canoes that were capable of ocean voyages, and they had the ability to sail them.’ says Goeff Irwin, a professor of archaeology at the University of Auckland. Those sailing skills, he says, were developed and passed down over thousands of years by earlier mariners who worked their way through the archipelagoes of the western Pacific, making short crossings to nearby islands. The real adventure didn’t begin, however, until their Lapita descendants sailed out of sight of land, with empty horizons on every side. This must have been as difficult for them as landing on the moon is for us today. Certainly it distinguished them from their ancestors,but what gave them the courage to launch out on such risky voyages?The Lapita’s thrust into the Pacific was eastward, against the prevailing trade winds, Irwin notes. Those nagging headwinds, he argues, may have been the key to their success. ‘They could sail out for days into the unknown and asses the are, secure in the knowledge that if they didn't find anything, they could turn about and catch a swift ride back on the trade winds. This is what would have nude the whole thing work.’ Once out there, skilled seafarers would have detected abundant leads to follow to land: seabirds, coconuts and twigs carried out to sea by the tides, and the afternoon pile-up of clouds on the horizon which indicates an islands in the distance.For returning explorers, successful or not, the geography of their own archipelagoes would have provided a safety net. Without this to go by, overshooting their home ports, getting lost and sailing off into eternity would have been all too easy. Vanuatu, for example, stretches more than 500miles in a northwest-southeast trend, its scores of intervisible islands forming a backstop for mariners riding the trade winds home.All this presupposes one essential detail, says Atholl Anderson, professor of prehistory at the Australian National University: the Lapita had mastered the advanced art of sailing against the wind. ‘And there’s no proof they could do any such thing,’ Anderson says, ‘There has been this assumption they did, and people have built canoes to re-create those early voyages based on that assumption. But nobody has any idea what their canoes looked like or how they were rigged.’Rather than give all the credit to human skill, Anderson invokes the winds of chance. ElI Nińo, the same climate disruption that affects the Pacific today, may have helped scatter the Lapita, Anderson suggests. He points out that climate data obtained from slow-growing corals around the Pacific indicate a series of unusually frequent EI Nińo around the time of the Lapita expansion. By reversing the regular east-to west flow of the trade winds for weeks at a time, these super EI Nińos’ might have taken the Lapita on long unplanned voyages.However they did it, the Lapita spread themselves a third of the way across the Pacific, then called it quits for reasons known only to them. Ahead lay the vast emptiness of the central Pacific and perhaps they were too thinly stretched to venture farther. They probably never numbered more than a few thousand in total, and in their rapid migration eastward they encountered hundreds of islands ― more than 300 in Fiji alone.Questions 27― 31Complete the summary using the list of words and phrases, A-J, below.Write the correct letter, A-J, in boxes 27-31 on your answer sheet.The Éfaté burial siteA 3,000-year-old burial ground of a seafaring people called the Lapita has been found on an abandoned 27 _______ on the Pacific island of Éfaté. The cemetery, which is a significant 28 _______ , was uncovered accidentally by an agricultural worker.The Lapita explored and colonised many Pacific islands over several centuries. They took many things with them on their voyages including 29 _______ and tools.The burial ground increases the amount of information about the Lapita available to scientists. A team of researchers, led by Matthew Spriggs from the American National University, are helping with the excavation of the site. Spriggs believes the 30 _______ which was found at the site is very important since it confirms that the 31 _______ found inside are Lapita.A proofB plantationC harbourD bonesE dataF archaeological discoveryG burial urnH sourceI animalsJ mapsQuestions 32―35Choose the correct letter, A, B, C or D.Write the correct letter in boxes 32-35 on your answer sheet.32 According to the write, there are difficulties explaining how the Lapita accomplished their journey becauseA the canoes that have been discovered offer relatively few clues.B archaeologists have shown limited interest in this area of research.C little information relating to this period can be relied upon for accuracy.D technological advances have altered the way such achievements are viewed.33 According to the sixth paragraph, what was extraordinary about the Lapita?A They sailed beyond the point where land was visible.B Their cultural heritage discourages the expression of fear.C They were able to build canoes that withstood ocean voyages.D Their navigational skills were passed on from one generation to the next.34What does ‘This’ refer to in the seventh paragraph?A the Lapita’s seafaring talentB the Lapita’s ability to detect signs of landC the Lapita’s extensive knowledge of the regionD the Lapita’s belief they would be able to return home35 According to the eighth paragraph, how was the geography of the region significant?A It played an important role in Lapita culture.B It meant there were relatively few storms at sea.C It provided a navigational aid for the Lapita.D It made a large number of islands habitables.Questions 36―40Do the following statements agree with the views of the writer in Reading Passage 3? In boxes 36-40 on your answer sheet,writeYES if the statement agrees with the views of the writerNO if the statement contradicts the views of the writer NOT GIVEN if it is impossible to say what the writer thinks about this36It is now clear that the Lapita could sail into a prevailing wind.37 Extreme climate conditions may have played a role in Lapita migration.38The Lapita learnt to predict the duration of EI Nińos.39It remains unclear why the Lapita halted their expansion across the Pacific.40 It is likely that the majority of Lapita settled on Fiji.。

3GPP 5G基站(BS)R16版本一致性测试英文原版(3GPP TS 38.141-1)

3GPP 5G基站(BS)R16版本一致性测试英文原版(3GPP TS 38.141-1)

4.2.2
BS type 1-H.................................................................................................................................................. 26
4.3
Base station classes............................................................................................................................................27
1 Scope.......................................................................................................................................................13
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TY-030B-01TensileTesting

TY-030B-01TensileTesting

TY-030B-01TEST METHOD M E T H O D F O R D E T E R M I N A T I O N O F T E N S I L ES T R E N G T H , T E N S I L E M O D U L U S O F E L A S T I C I T Y ,A N D E L O N G A T I O N A T B R E A K1.SCOPET h i s m e t h o d c o v e r s d e t e r m i n a t i o n o f t e n s i l e s t r e n g t h ,t e n s i l e m o d u l u s o f e l a s t i c i t y,a n d e l o n g a t i o n a t b r e a k o f c a r b o n f i b e r y a r n w h i c h h a s b e e n r e s i n -i m p r e g n a t e d a n d t h e n c u r e d.2.PROCEDURE2.1IMPREGNATION2.1.1P r e p a r a t i o n o f i m p r e g n a t i n g r e s i n(1)B l e n d 30g o f B F 3M E A w i t h 40g o f a c e t o n e o r e t h a n o l ,a n d s t i r u n t i l t h e y a r e c o m p l e t e l y d i s s o v e d.(2)A d d 1000g o f B a k e l i t e E R L 4221 r e s i n t o t h e a b o v e s o l u t i o na n d s t i r a g a i n u n t i l c o m p l e t e l y d i s s o l v e d.B a k e l i t e E R L 4221 e p o x y r e s i n i s a p r o d u c t o f U n i o nC a r b i d e C o r p o r a t i o n.2.1.2I m p r e g n a t i o n(1)A s a m p l e b o b b i n i s s e t o n a b o b b i n h o l d e r,t h e n t h e s a m p l e c a r b o nf i b e r y a r n i s p u l l e d o u t ,d i p p e d i n a r e s i n b a t h a n d s q u e e z e d o v e rr o l l e r s.E x c e s s r e s i n i s w i p e d o f f a n d ,t h e n w o u n d o n t o af o u r-b r a n c h e d f r a m e.I m p r eg n a t i o n c o n d i t i o n s a r e a s f o l l o w s :Te m p e r a t u r e:25 t o 30˚CTe n s i o n :100 t o 200g /y a r n(e x a m p l e f o r T 300 3K a n d 6K )L i n e s p e e d :7 m /m i n.(d i t t o )(2)F i x a t a i l o n t h e f r a m e w i t h a d h e s i v e p a p e r.(3)W i p e o f f e x c e s s r e s i n o n t h e y a r n w i t h p a p e r.A -2T O R A Y C A R B O N F I B E R S A M E R I C A , I N C .Bobbin HolderResin Bath Squeeze Rollers Wiper Rollers WinderFig. 1 Impregnation ApparatusT O R A Y C A R B O N F I B E R S A M E R I C A , I N C .6 H u t t o n C e n t r e D r i v e ,S u i t e #1270,S a n t a A n a ,C A 92707 T E L :(714) 431-2320 FA X :(714) 424-0750S a l e s @To r a y c f a.c o m Te c h n i c a l @To r a y c f a.c o m w w w.t o r a y.c o m2.2C U R I N GT h e i m p r e g n a t e d s t r a n dw o u n d o n t h e f r a m e i s c u r e di n a n o v e n a t 130 ±5˚C f o r30 t o 35 m i n u t e s.2.3T h e c u r e d s t r a n d i s c u t a tthe fixed length (approx.30cm)f o r m e a s u r e m e n t.Te s tS p e c i m e n s w i t h b e n d s ,w i t hb e a d s o fc u r ed re s i n ,o rwith imperfect penetra tion ofr e s i n a m o n g t h e f i l a m e n t s ,a r e d i s c a r d e d.2.4T E N S I L E T E S TA t e n s i l e t e s t e r i s s h o w n i n F i g.3.T h e r e a r e t w o m e t h o d s f o r d e t e r m i n a t i o n o f t e n s i l e m o d u l u s o f e l a s t i c i t y.U n l e s s o t h e r w i s e s p e c i f i e d ,m e t h o d A i s u s e d.2.4.1M e t h o d A(1)E x t e n s i o n o f d i s t a n c e b e t w e e n c h u c k s i s m e a s u r e d.G r a d i e n t o f l o a dv s.e l o n g a t i o n d e t e r m i n e s t e n s i l e m o d u l u s o f e l a s t i c i t y w i t hc a l i b r a t i o n o f m e c h a n i c a l s y s t e m e x t e n s i o n.(2)A t e s t s p e c i m e n i s p l a c e d o n g r i p s o f t h e t e n s i l e t e s t e r,w h o s ec h u c k f a c e m a t e r i a l i s r u b b e r o r s o f t g r i p p i n g s h e e t.S a nd p a pe r c a nb e u s e d b e t w e e n t h ec h u c k f a c e a nd s pe c i m e n w h e n s l i p p a g e i so b s e r v e d b e t w e e n t h e s a m p l e a n d c h u c k f a c e.G a g e l e n g t h :200m mFig. 2 RollersMotor or HydraulicMovable grip (cross head)Stationary gripExtensometerStrain MeterSampleClampStationary gripFig. 3 Tensile TesterTY-030B-01TEST METHOD(3)The specimen is loaded to failure.Crosshead speed is 0.5 to 1.0mm/sec.(4)M e a s u r e d y i e l d(m a s s p e r u n i t l e n g t h)a n d d e n s i t y d a t a o f t h es a m p l e a r e u s e d f o r c a l c u l a t i o n o f c r o s s s e c t i o n a l a r e a o f y a r n.(r e f e r T Y-030B-02,-03)(5)C a l c u l a t i o nTe n s i l e s t r e n g t h,t e n s i l e m o d u l u s a n d p e r c e n t e l o n g a t i o n a r ec a l c u l a t ed a s f o l l o w s:T S=B L ST M=B Lx(L+T)x10-3 S C E–K·B LE=C E–K·B Lx100 (L+T)w h e r e,T S:t e n s i l e s t r e n g t h(k g f/m m2)T M:t e n s i l e m o d u l u s(103k g f/m m2)E:e l o n g a t i o n(%)B L:b r e a k i n g l o a d(k g f)L:g a g e l e n g t h(c h u c k s p a n,m m)T:s a g(m m)C E:c a l c u l a t e d e l o n g a t i o n(m m)K:s y s t e m c o m p l i a n c e(m m/k g f)S:c r o s s s e c t i o n a l a r e a o f t h e s a m p l e(m m2)S=Wx1/1000ρw h e r e,W:y i e l d o f t h e s a m p l e(g/1000m)ρ:d e n s i t y o f t h e s a m p l e(g/c m3)R e l a t i o n a m o n g t h e s e s y m b o l s i s i l l u s t r a t e d i n F i g.4.D e t e r m i n a t i o n o fs y s t e m c o m p l i a n c e K i s s h o w n i n F i g.5.C E/B L o f t h e s a m e y a r n s a m p l e a r e m e a s u r e d f o r t h r e e d i f f e r e n t g a g el e n g t h.K i s d e t e r m i n e d a s t h e e x t r a p o l a t e d v a l u e o f C O/B L a t z e r o g a g el e n g t h.(6)R e f e r e n c eJ I S R7601 Te s t i n g M e t h o d s f o r C a r b o n F i b e r sA-3 T O R A Y C A R B O N F I B E R S A M E R I C A,I N C.T O R A Y C A R B O N F I B E R S A M E R I C A , I N C .6 H u t t o n C e n t r e D r i v e ,S u i t e #1270,S a n t a A n a ,C A 92707 T E L :(714) 431-2320 FA X :(714) 424-0750S a l e s @To r a y c f a.c o m Te c h n i c a l @To r a y c f a.c o m w w w.t o r a y.c o m2.4.2M e t h o d BIn this method,extension of the specimeni s d i r e c t l y m e a s u r e d b y a n e x t e n s o m e t e r.Tensile modulus of elasticity is calcula tedas the gradient of load versus elonga tion.T h e t e n s i l e m o d u l u s i s c a l c u l a t e d a t t h ep r e s c r i b e d r a n g e o f p e r c e n t e l o n g a t i o n.Fo r e x a m p l e ,f o r T 300J f i b e r s f r o m 0.3%t o 0.7% s t r a i n i s u s e d.(1)A t e s t s p e c i m e n i s s e t o n g r i p s o ft h e t e n s i l e t e s t e r,w h o s e c h u c k f a c em a t e r i a l i s r u b b e r o r s o f t g r i p p i n gs h e e t.Sand pa per can be used between thec h u c k f a c e a nd s pe c i m e n ,w h e ns l i p p a g e i s o b s e r v e d b e t w e e n t h esample and chuck face.An extensometeri s a t t a c h e d t o t h e s p e c i m e nb e t w e e n t h e g r i p s.G a g e l e n g t h f o r s t r e n g t hm e a s u r e m e n t :200m mGa ge length of theextensometer f o r e x t e n s i o nm e a s u r e m e n t :150m m LoadElongation Gage Length L (mm)Fig. 4Load-elongation relationship Fig. 5 Determination of system complianceM E T H O D ALoad Elongation Fig. 6 Load-elongation relationshipM E T H O D BTY-030B-01TEST METHOD(2)The specimen is loaded to failure.Crosshead speed is 0.5 to 1.0mm/sec.(3)I n o r d e r t o c a l c u l a t e c r o s s s e c t i o n a l a r e a o f t h e s a m p l e,y i e l d(m a s s p e r u n i t l e n g t h)a n d d e n s i t y d a t a o f t h e s a m p l e a r e u s e d.(r e f e r t o T Y-030B-02,-03)(4)C a l c u l a t i o nTe n s i l e s t r e n g t h,t e n s i l e m o d u l u s o f e l a s t i c i t y a n d p e r c e n t e l o n g a t i o na r e c a l c u l a t e d a s f o l l o w s:T S=B L ST M=(L2–L1)x1x1 (E2–E1)S10E=T Sx1 T M10w h e r e,T S:t e n s i l e s t r e n g t h(k g f/m m2)T M:t e n s i l e m o d u l u s o f e l a s t i c i t y(103k g f/m m2)E:e l o n g a t i o n(%)B L:b r e a k i n g l o a d(k g f)E2–E1:p r e s c r i b e d p e r c e n t e l o n g a t i o n s(%)L2–L1:L o a d s c o r r e s p o n d i n g t o E1a n d E2(k g f) S:c r o s s s e c t i o n a l a r e a o f t h e s a m p l e(m m2)S=Wx1/1000ρw h e r e,W:y i e l d o f t h e s a m p l e(g/1000m)ρ:d e n s i t y o f t h e s a m p l e(g/c m3)A-4 T O R A Y C A R B O N F I B E R S A M E R I C A,I N C.We believe this information to be reliable and the best currently available on the subject.Revisions will be made as additional information and experience are obtained.Toray Industries,Inc.and Toray Carbon Fibers America,Inc.make no guarantee of results and assumes no obligation or liability whatsoever in connection with this publication.Nothing herein is intended as a recommendation to use our products so as to infringe any existing patents.T O R A Y C A R B O N F I B E R S A M E R I C A,I N C.6H u t t o n C e n t r e D r i v e,S u i t e#1270,S a n t a A n a,C A92707 T E L:(714)431-2320FA X:(714)424-0750S a l e s@To r a y c f a.c o m Te c h n i c a l@To r a y c f a.c o m w w w.t o r a y.c o m。

Gentex 9123 9223 系列光电烟感120VAC和220VAC,9V电池备份,单 多站烟感

Gentex 9123 9223 系列光电烟感120VAC和220VAC,9V电池备份,单 多站烟感

9123 / 9223 SeriesPhotoelectric Smoke Alarm120 VAC and 220 VAC with 9V Battery Back-Up Single/Multiple Station Smoke Alarm 24 units per carton, 34 pounds per cartonApplicationsThe 9123/9223 Series of photoelectric smoke alarms is designed for residential andcommercial residential applications, including homes, apartments, hospitals, hotelsand motels. Available in several different models, the 9123/9223 Series is engineeredto virtually eliminate nuisance alarms and deliver outstanding performance whereverreliable fire protection is required.The 9123/9223 Series is provided with a 9V alkaline battery for back-up in the eventbuilding power is lost. The Gentex 9123/9223 Series provides an exclusive patentedthree-position test feature that simulates a 0.85% and 3.5% actual smoke conditionin full compliance with NFPA 72 and UL Standards. Options include self-restoring135°F integral or isolated heat thermals and Form A/Form C dry contacts for remoteannunciation.The 9123/9223 smoke alarms are ANSI/UL 217 and ANSI/UL 1730 listed and arewarranted for one year from date of purchase.Standard Features• Available in 120VAC and 220VAC models with 9V battery back-up• Photoelectric smoke sensing technology• Horn frequency 3100 Hz (nominal)• 90dBA temporal 3 evacuation piezo horn• Nominal 2.5% sensitivity• Patented three-position test switch• Relays operate on battery back-up• Quick-disconnect wiring harness• Interconnect with Gentex tandem capable smoke alarms• Non-latching (self restoring) alarm• 5-to-1 signal-to-noise ratio• Pulsing LED sensing chamber• Fully insect screened• Easy Wash® on-site maintenance washing program• Red LED pulses every 30 seconds, green LED for AC power on• Mounting hardware adapts to standard junction boxes• Dust cover to prevent contamination during installation•Low or missing battery indicator• One year warranty from date of purchaseProduct Listings• ANSI/UL 217 and ANSI/UL 1730 Listed• BS+A/MEA #285-91-E• BFP (City of Chicago)• MSFM Listing #1929• Hong Kong FSD Listed (9223 Series only)Product Compliance• NFPA 72• IBC/IFC/IRC• Quality Management System is certified to:ISO 9001:20089123 / 9223 SeriesModel Part Number Voltage (VAC)Integral135ºF ThermalIsolated135ºF ThermalTandemUp To 12 UnitsTandemUp To 6 UnitsForm A/CContacts9123917-0012-002120 VAC•9123T917-0013-002120 VAC••9123H917-0014-002120 VAC••9123F917-0015-002120 VAC••9123TF917-0017-002120 VAC•••9123HF917-0016-002120 VAC•••9223917-0032-002220 VAC•9223T917-0033-002220 VAC••9223H917-0034-002220 VAC••9223F917-0035-002220 VAC••9223TF917-0037-002220 VAC •••9223HF917-0036-002220 VAC•••Notes• Series available in round configuration only• When testing 9123 Series, it may take up to 16 seconds longer for smoke alarm to go in or out of alarm mode• It is recommended that 9123/9223 Series smoke alarmbe tested weekly• Refer to Technical Bulletin 002 for Easy Wash® on site washing instructions• Units produce a temporal 3 audible alarm Electrical SpecificationsOperating Voltage (9123) ................................................120 VAC, 60 Hz Operating Voltage (9223) ................................................220 VAC, 50 Hz Operating Current .............................................................0.045 amps Operating Current (Relay Options) ..............................0.070 amps Operating Ambient Temp Range .................................40°F to 100°F Alarm Horn Rating .............................................................90dBA at 10 feet Nominal Sensitivity ...........................................................2.5% obscuration “F” Auxiliary Relay ..............................................................1 Form A and1 Form C (0.6 amp)“T” Integral Thermal (Self-Restoring) ..........................135°F at 50 feet “H” Isolated Thermal Form A (Self-Restoring) .........135°F at 50 feet Size ..........................................................................................Diameter: 6.5” OA(5.75” at base)Depth: 2.625”Secondary Power Source ................................................Alkaline 9V battery Duracell® MN 16049123 / 9223 Series - Photoelectric Smoke AlarmTandem Wiring DiagramRelay ModelsModels: 9123F, 9123TF, 9223F, 9223TF Isolated Thermal withOptional Accessory ContactsModels: 9123H, 9123HF, 9223H, 9223HFLimitations• Maximum of 12 alarms (9123, 9123T, 9123H, 9223, 9223T, 9223H) may be connected together• Do not exceed 125 ft. between each alarm• Do not exceed 1125 ft. between the first and last alarm • Note: Gentex smoke alarms can not be interconnected to alarms from other manufacturers• A maximum of six (6) alarms with a relay may be tandem interconnected (9123F, 9123TF, 9123HF, 9223F, 9223TF, 9223HF)Caution• RED/YELLOW wire to be capped when not in use • This wire is for tandem connection only • Do not connect to any other circuitRelay Contacts Rated Load Resistive• 1.0 AMP @ 24 VDC• 0.6 AMP @ 125 VAC MAX • 0.3 AMP @ 220 VAC MAXBRN Smoke Alarm Heat SensorAccess ContactsAlarm Contacts Tandem PowerH F O n l yBRN GRAY GRAY YEL ORN BLU VIO VIO/BLK RED/YEL BLKWHTGRAY Supervision WiresAccess ContactsAlarm ContactsTandem PowerGRAY YELORN BLUVIO VIO/BLK RED/YEL BLK WHTQuickDisconnect Type Plug123123123120 Volts 60 HzElectrical BoxElectrical BoxNeutralWHT TandemRED /YEL To additionalGentex smoke alarmsHot BLKElectrical BoxSmoke AlarmSmoke AlarmSmoke AlarmThe photoelectric smoke alarm shall be a Gentex model 9123/ 9223 or approved equal which shall provide at least the following features and functions.• Nominal sensitivity shall be 2.5%• The alarm shall utilize an infrared LED sensing circuit which pulses in 4 to 5 second intervals when subjected to smoke. After 2 consecutive pulses in smoke, the alarm will activate.• The alarm shall have a 9V alkaline battery as a back-up in the event building power is lost.• The 9V battery impedance shall be verified by the circuitof the smoke alarm.• The alarm shall provide an indicator when the batteryis low in power, or high impedance, or is missing.• The alarm shall provide minimum 5-to-1 signal-to-noise ratio in the optics frame to assure stability of operation in environments of high RF and transient conditions.• The sensing chamber shall be fully screened to prevent entrance of small insects, thus reducing the probabilityof false alarms.• A temporal 3 piezo alarm rated at 90dBA at 10ft.• A visual LED monitor (condition indicator) will slowpulse in normal operation and rapid pulse in alarm.• An easily accessible test knob shall be provided. The test knob in the TEST position will simulate an actual smoke condition of approximately 3.5% causing the detector to alarm within 20-36 seconds. It will also have the capability of testing to 0.85% as arequired minimum. A magnetic switch closure or other switch closure, or smoke generating equipment which does not scatter the light beam or test sensitivity is not sufficient,as indicated in National Code.• The detector shall have interconnect capabilities of up to 12 units or 6 units with relay.• The alarm shall have interconnection capabilities of12 units on 9123/9123T/9123H/9223/9223T/9223H andshall have interconnection capabilities of 6 units on9123F/9123TF/9123HF/9223F/9223TF/9223HF.• The manufacturer shall provide other compatible alarm models with the following optional features: a) 135°F isolated thermal with normally opened contact for remote connection to local alarm or annunciator; b) 135°F integral thermal; c) auxiliary Form A/Form C relay contacts for initiating remote functions and annunciation; d) relay option that is capable of activation by tandem interconnect wire. Thermal sensor shall be self-restoring.• Unit must be ANSI/UL 217 and ANSI/UL 1730 listed for both wall and ceiling mount.• Unit shall be listed by Underwriters Laboratories.All equipment shall be completely factory assembled, wired and tested, and the contractor shall be prepared to submit a certified letter testifying to this condition. Alarms which do not meet all of the requirements of this specification will not be considered.9123 / 9223 Series Photoelectric Smoke Alarm Architect & Engineering Specifications。

MBN_10447_2010-05_EN_Quality Management Standard-Elektrics-Elektronics for Mercedes-Benz Cars_nng

MBN_10447_2010-05_EN_Quality Management Standard-Elektrics-Elektronics for Mercedes-Benz Cars_nng

10447 Mercedes-Benz MBN Company Standard Date published: 2010-05Folder: 22Supersedes: A212 000 18 99Total no. of pages (including Annex): 49Person in charge: Matthias GeigerPlant 050; Dept.: MBC/QEEDate of Translation: 2010-11 Phone: +49 7031 90 49 400Quality Management StandardElectrics/Electronicsfor Mercedes-Benz CarsQualitätsmanagement-Norm Elektrik/Elektronik für Mercedes-Benz CarsForewordThis Quality Management Standard Electrics/Electronics for Mercedes-Benz Cars describes therequirements specified by Daimler AG for suppliers of electrical/electronic components and electri-cal/electronic control units for Mercedes-Benz Cars.This Standard is applicable in addition to the component requirement specifications for the devel-opment, manufacture and series production of this component by a contractor of Daimler AG.ChangesFirst editionNOTE: This translation is for information purposes only.The German version shall prevail above all others.Copyright Daimler AG 2010Contents1Scope (5)2Normative references (6)3Terms and definitions (7)3.1List of abbreviations (7)3.2Nomenclature (8)4General requirements (9)4.1Contacts at Daimler AG (9)4.2Contacts at the supplier and its sub-suppliers (10)4.3Key processes (10)5Preventive maturity level management (11)5.1Start of preventive maturity level management (11)5.2Scope (11)5.3Tracking of sub-supplier maturity level (12)5.4Changes following start of production (12)5.4.1Process and sub-process relocation (12)5.4.2Replacement or exchange of machines or equipment (12)5.4.3Change of a sub-supplier (13)6Detection of anomalies (14)7Process capability and product reliability (15)7.1Proof of machine and process capability for SMT processes (15)7.1.1Machine and process capability of paste printer (15)7.1.2Machine capability placement machines (15)7.1.3Verification of solder profile (16)7.2Proof of reliability of the assembly and connection technology (16)7.3Proof of reliability of the devices used (16)7.4Board bending test (17)7.5Requalification (18)7.5.1Complete repeat of the environmental and life tests (18)7.5.2Q-Review Environment E/E (18)8Manufacturing processes for electronic components (20)8.1Storage (20)8.1.1Moisture sensitive devices (20)8.2Printed circuit board magazines (21)8.3Transportation of devices and components (21)8.4Soldering paste printing (21)8.4.1Initial part approval during series production (21)8.4.2Soldering paste (21)8.4.3Paste printer (22)8.4.4Cleaning of the stencil (22)8.4.5Cleaning of circuit boards following soldering paste printing (22)8.4.6Mechanical stress in double-sided PCB assembly (23)8.5PCB assembly (23)8.5.1Initial part approval (23)8.5.2Reel change (23)8.5.3Mechanical stress (23)8.5.4Process control (23)8.5.5Maintenance (23)8.6Assembly and connection technology (24)8.6.1Reflow soldering (24)8.6.1.1Machine malfunctions (25)8.6.1.2Temperature profile (25)8.6.2Press-fit technology (25)8.6.3Selective soldering with mini-wave (26)8.6.3.1Flux (26)8.6.3.2Temperature pretreatment and temperature gradient (26)8.6.3.3Temperature monitoring (27)8.6.3.4Machine malfunctions (27)8.6.3.5Solder residue (27)8.6.3.6Solder bath (27)8.6.3.7Solder filling level (27)9Rework (28)10Test technology in series production (29)10.1Inspection of soldered joints (29)10.1.1Inspection of paste printing (29)10.1.2Inspections after reflow soldering (29)10.1.3Inspections after selective soldering (30)10.1.4Manual visual inspections (30)10.2In-circuit test (30)10.3Contacting of components (31)10.4End-of-line test (31)10.5Test parameters (32)10.6Mechanical interfaces (32)10.7Product audit (32)10.7.1Temperature cycle test (33)10.7.2Additional component-specific tests (33)10.7.3Changes (34)10.8Early defect detection (34)10.8.1Realization of early defect detection (34)10.8.2Active run-in (34)10.9Test coverage analysis (35)10.10Evaluation and reporting of internal test results (36)10.11Haptic measurements (36)10.12Testing of function, switch and controls illumination (37)10.13Noise testing (37)10.14Process documentation and process records (38)10.14.1Soldering paste printing (38)10.14.2Placement machines (38)10.14.3Reflow soldering (38)10.14.4Selective soldering with mini-wave (38)10.14.5Rework (39)10.14.6Test parameters (39)11Mechanical manufacturing processes (40)11.1Circuit board separation (40)11.1.1Milling (40)11.1.2Punching (V-cutting) (40)11.1.3Sawing (40)11.1.4Laser cutting (41)11.2Assembly and screw-fastening processes (41)11.3Zero Insertion Force (ZIF) connectors (41)11.3.1Manual joining of zero insertion force connectors (42)11.3.2Semi or fully automatic joining of zero insertion force connectors (42)11.3.3Testing of the connection of zero insertion force connectors (42)11.3.4Opening of the plug connection of zero insertion force connectors (42)12Traceabilty of devices and components (43)12.1Incoming goods (43)12.2PCB assembly (43)12.3Tests (44)12.4End-of-line test (44)12.5Outgoing goods (44)12.6Rework (44)13ESD (45)13.1ESD protection measures in electronics production (45)13.2Personnel grounding (45)13.3Rework (45)14Flashing of components (46)14.1Handling (46)14.2Contacting and flashing (46)14.3Testing and traceability of flashed components (46)14.4Capacity of the flashing process (47)15Failure analysis (48)15.1Analysis reports (48)15.2Priority failures (48)15.3NTF failures (complaints) (48)15.4Failure analysis on site (48)16On-site support (49)16.1Professional requirements for staff (49)16.2Time-related requirements (49)16.3Other requirements (49)1 ScopeThis Quality Management Standard Electrics/Electronics applies irrespective of the model to all electri-cal/electronic components in general.2 Normative referencesMB Special Terms Mercedes Benz Special Termsof Electronic AssembliesANSI/IPC-A-610D AcceptabilityIPC/JEDEC J-STD-033B.1 Handling, Packing, Shipping and Use of Moisture/ReflowSensitive Surface Mount DevicesDIN EN ISO 9453 Soft Solder Alloys – Chemical Compositions and FormsA2110039899 Design Rules for E/E ComponentsDIN EN 61340-5-1 Protection of Electronic Devices from Electrostatic Phenom-ena — General RequirementsIEC/TR 61340-5-2 Protection of Electronic Devices from Electrostatic Phenom-ena – User GuideDIN EN 61340-4-5 Standard Test Methods for Specific Applications – Methodsfor Characterising the Electrostatic Protection of Footwearand Flooring in Combination with a PersonDIN EN 61340-4-3 Standard Test Methods for Specific Applications – Footwear AEC-Q100 Stress Qualification for Integrated CircuitsAEC-Q101 Stress Test Qualification for Discrete SemiconductorsAEC-Q200 Stress Test Qualification for Passive ComponentsAEC-Q004 Zero Defects Guideline (Draft version)ANSI/IPC J-STD-001D Requirements for Soldered Electrical and Electronic Assem-bliesMBN 10448 Field Failure Analysis3 Terms and definitions3.1 List of abbreviationsTwo-dimensional2DThree-dimensional3DAEC Automotive Electronic Council (body for quality standards in the automotive indus-try)InspectionOpticalAutomatedAOI(Ausführungsvorschrift)regulationAVImplementationBGA Ball Grid Array componentsBR Vehicle model series (Baureihe)cmk Short-term process capabilitycapabilityprocessLong-termcpksupplyspecification (Liefervorschrift)Daimler-BenzDBLDS Identification and documentation of safety relevancedocumentation of certification relevanceandIdentificationDZE/E component Electrical/electronic componentProgrammable Read-Only MemoryEEPROM ElectricallyErasableX-rayspectroscopyEnergy-dispersiveEDXEOL End Of Line testOverStressEOSElectricalDischargeElectroStaticESDFMEA Failure Mode and Effects AnalysisLevelingAirHotHALHIL Hardware In the LoopHardWareHWStandardizationISOforOrganisationInternationalCircuitsIC IntegratedIn-Circuit-TestICTspecifications (Komponentenlastenheft)requirementComponentKLHMBN Mercedes-Benz standard (Mercedes-Benz Norm)SystemDevelopmentMercedes-BenzMDSInterfaceMan-MachineMMIMSD Moisture Sensitive DeviceLevelSensitiveMSLMoistureSystemProductionMercedes-BenzMPSMTTF Mean Time To FailureNTF No Trouble Foundprocess and product approvalPPAProductioncapabilityprocessPreliminaryppkPRG Product maturity level (Produkt-Reifegrad)GateQGQualityQualityManagementQMStatusQ-Status QualityMemoryAccessRandomRAMMemoryOnlyReadROMTemperatureRoomRTUnitControlCUMountedTechnologySurfaceSMTSOP Start of ProductionSoftWareSWTechnologyHoleThroughTHT3.2 NomenclatureBelow, electrical/electronic components and electrical/electronic control units are termed "components" for the reader’s convenience.Below, the contractor of Daimler AG is termed "supplier".Below, the sub-components of components such as circuit boards, electronic devices (e.g. controllers, transceivers, micromechanical semiconductors) and mechanical units (e.g. housings) are termed "de-vices" for the reader’s convenience.Below, requirements for documentation and the recording of data are specified. In this context, "docu-ment" refers to instructions and specifications (e.g. work instructions, process descriptions, etc). The term "record" refers to evidential data (e.g. completed checklists, audit evidence, etc).4 GeneralrequirementsFor safety requirements, homologation and quality, the existing statutory requirements and laws shall be complied with. In addition, the relevant requirements of Daimler AG apply.All materials, procedures, processes, components, and systems shall conform to the current regulatory (governmental) requirements regarding regulated substances and recyclability.This Quality Management Standard Electrics/Electronics makes reference to applicable laws, standards and regulations etc. The supplier shall be responsible for compliance with all laws, standards and regula-tions and for the development and production of the component in line with the state of the art. In this con-text, due consideration shall be given to the fact that the vehicles of Daimler AG containing this compo-nent are sold worldwide.This Quality Management Standard Electrics/Electronics makes reference to other applicable documents of the component requirement specifications (KLH) (specifications, test methods, implementation regula-tions, instructions of Daimler AG). Where this Quality Management Standard Electrics/Electronics contains deviating or contradictory information compared with other standards, specifications or implementation regulations, the more severe specification shall apply. In case of doubt, clarifying agreements following discussions with Daimler AG Quality Management shall be set down in writing.The supplier shall supply conforming products to Daimler AG, and the supplier shall maintain the zero-defect target.If the supplier is aware of measures or alternatives serving to increase quality or reliability, the supplier shall notify these to Daimler AG Quality Management.All information and documents associated with the development, manufacture and production of the com-ponent shall be treated confidentially.4.1 Contacts at Daimler AGThe responsible component developer and other contacts at Daimler AG are listed in the component re-quirement specifications (KLH).Mercedes-Benz Cars Quality Management is divided into two units:- Preventive Quality Management (Prevention) and- Quality Management Production in the worldwide Daimler assembly, body, paintwork and stamp-ing plants (e.g. Sindelfingen, Bremen, Tuscaloosa, South Africa etc.).During the development phase (requirement specification phase up to the launch of the component in production), a staff member from Prevention is the responsible quality contact for the supplier. Together with the responsible staff member from Prevention, the supplier shall hold coordination discussions re-garding quality management requirements. The supplier shall seek approval from the responsible staff member from Prevention for any deviations from these quality management requirements.During the production phase (launch of component in production up to discontinuation of production), a Quality Management staff member from each assembly, body, paintwork and stamping plant is the re-sponsible quality contact for the supplier. The supplier shall seek approval for all changes to the compo-nent or production process during the production phase from the responsible Quality Management staff member from the assembly, body, paintwork and stamping plants. In the event of deviations from the re-lease status of the component, the supplier shall present appropriate measures and samples and have any changes approved.Any deviation from the requirements of this Quality Management Standard Electrics/Electronics are sub-ject to the written approval of Daimler AG Quality Management.4.2 Contacts at the supplier and its sub-suppliersThe supplier shall submit an organizational diagram to Daimler AG Quality Management showing all per-sons responsible for the project and their functions.The supplier shall reveal the complete supply chain of devices for the project to Daimler AG Quality Man-agement. In this process, the supplier shall document the scope of supply and supplier name of each de-vice.4.3 Key processesTo facilitate the successful implementation of the project, the supplier shall provide evidence of docu-mented process structures for the following key processes during the concept presentation:1. Requirements analysis process2. Test strategy process3. Configuration and change management process4. Problem analysis process5. Project management5 Preventive maturity level managementThe objective of preventive maturity level management is to recognize quality-related problems and defi-cits concerning the product and/or production process as early as during the development phase of the component and to be able to initiate countermeasures. Timely completion of the project and defect-free implementation of all specified functions are the top priorities for Daimler AG.The supplier shall document and maintain a preventive maturity level management system. As part of this system, the supplier shall determine and record characteristic data (metrics, process capability indices, inspections, etc.).In this context, all company units of the supplier involved with the product creation process shall be sub-ject to the maturity level management system.Assessment of the maturity level shall be based on the specified quality targets and quality criteria throughout the product and process development process.The supplier shall document compliance with and fulfillment of all requirements from the component re-quirement specifications (KLH) and this Standard.To track all activities during development, the supplier shall maintain a list of open issues, and grant Daim-ler AG Quality Management access to this list on request.The supplier shall submit regular reports to Daimler AG Quality Management regarding maturity level pro-gress. The supplier shall document maturity level reports in writing. The supplier shall record the maturity level reports for the Quality Gates (according to MDS) and submission of A, B, C, D and PPF samples in writing.5.1 Start of preventive maturity level managementThe supplier shall initiate preventive maturity level management at the time of project start - immediately following the commencement of hardware and software development and the start of the production proc-ess.5.2 ScopeThe supplier shall coordinate and document the scope of preventive maturity level management with Daimler AG Quality Management.The preventive maturity level tracking during the product creation process includes the monitoring of the degree of fulfillment of all requirements. In this context, the supplier shall document and record the (func-tional and non-functional) requirements for the component and the production process during the devel-opment phase of the component.The supplier shall carry out an assessment on the basis of the degree of implementation of the require-ments at the relevant project date. The maturity level is divided into four stages:- Requirement not implemented by the deadline- Requirement is in the process of being implemented- Requirement has been implemented by the deadline- Requirement has been implemented and tested successfully by the deadline5.3 Tracking of sub-supplier maturity levelThe supplier shall document and implement a preventive maturity level management system at all sub-supplier companies involved in the project (Tier 2, Tier 3, …).The supplier shall inform Daimler AG Quality Management of the status of the preventive maturity level management if there is a risk of the sub-suppliers involved in the project failing to reach the project objec-tive.On request, the supplier shall grant Daimler AG Quality Management access to records concerning the maturity level management of the sub-suppliers involved in the project.5.4 Changes following start of productionAny changes to the component or an existing manufacturing process shall be subject to the approval of Daimler AG Quality Management and be approved using a PPA process.The supplier shall qualify any change, e.g. in the event of changes to devices (material or manufacturing process of the device) or in the manufacturing process of the component. The supplier shall provide evi-dence of and document qualification in accordance with the component requirement specifications.Deviations from a complete qualification by the supplier shall be subject to the approval of Daimler AG Quality Management.Qualification shall be carried out using components manufactured on the production equipment at the se-rial production location.The documentation of changes shall be coordinated with Daimler AG Quality Management.The supplier shall adhere to a previously defined time frame for pre-advice to Daimler AG Quality Man-agement.In the cases indicated below, the supplier shall inform the following Daimler AG units: Quality Manage-ment, Development, Purchasing and Logistics.5.4.1 Process and sub-process relocationIn the case of any type of process and sub-process relocation, the supplier shall inform Daimler AG Qual-ity Management no later than 9 months before the intended implementation of the change. The supplier shall submit a relocation scenario and seek the approval of Daimler AG Quality Management for such scenario.This time frame also applies to the outsourcing of processes or sub-processes to sub-suppliers.5.4.2 Replacement or exchange of machines or equipmentIn the case of the replacement or exchange of machines or equipment or other systems, the supplier shall inform Daimler AG Quality Management no later than 3 months before the intended implementation of the change.5.4.3 Change of a sub-supplierIn the case of a change of a sub-supplier or manufacturer of a device of the component, the supplier shall submit a change scenario to Daimler AG Quality Management and seek the approval of Daimler AG Qual-ity Management for such scenario. The supplier should inform Daimler AG Quality Management no later than 6 months before the intended implementation of the change.6 Detection of anomaliesThe statistical detection of anomalies is intended for the detection of unusual features in the functionality or measurement parameters. These may be anomalies which lie within the specification limits provided, but are unusual compared to other components. The anomalies may point towards pre-damage to the component.In order to ensure the process capability and product reliability, the supplier shall document and use a method for the detection of anomalies, and provide evidence by means of records.To verify the process capability and product reliability, the supplier shall use this method, starting with the manufacture of initial samples, and create records. Evidence shall be provided no later than at the time of submission of the initial sample documentation.7 Process capability and product reliabilityIn accordance with VDA 2, the supplier shall provide evidence of the process capabilities for its production processes.For the deadline and the required values for the process capabilities, refer to MBST.At the time of submission of the initial samples, the supplier shall document the final evidence of the proc-ess capabilities and product reliabilities required.The initial samples shall be manufactured on production equipment and selected randomly.The supplier shall have any deviations from these specifications approved by Daimler AG Quality Man-agement.7.1 Proof of machine and process capability for SMT processesWithin the framework of the zero-defects strategy in relation to the customer, the supplier shall make every effort to prevent and detect nonconformances. From the point of view of customer satisfaction and with a view to ensuring the quality of the components, it is essential that nonconformances are detected as early as possible and eliminated. The focus shall therefore be on the process capability of the supplier's manufacturing process. This includes the determination of the ongoing process capability, the control of the production process and continuous process improvement.The supplier shall supply regular evidence of the process capabilities of production as a whole and each production process and maintain the appropriate records.7.1.1 Machine and process capability of paste printerThe supplier shall check the machine capability once every year and maintain the pertinent records.Evidence of the machine capability of the paste printer can be provided by means of a reference stencil. The relevant parameters for this purpose are the positioning accuracy in the x and y direction of the solder deposit.The supplier shall check the process capability of the paste printer with the product-specific original stencil and maintain appropriate records. During this process, the supplier shall document reference points and determine their positioning accuracies in x and y position as well as the volume. To do so, the supplier may use the paste AOI provided that the AOI measuring data can be analyzed.7.1.2 Machine capability placement machinesThe supplier shall check the machine capability every other year and maintain the pertinent records.The supplier shall check the machine capability using a glass board and glass devices or ceramic pads and maintain appropriate records. To prove capability, the supplier shall document the critical SMD shapes and test these.7.1.3 Verification of solder profileThe supplier shall verify that the solder profile determined allows each solder joint to reach the required soldering temperature and the required temperature profile. The supplier shall maintain appropriate re-cords.The supplier shall verify that "thermally critical" devices on the circuit board are not overheated. The sup-plier shall maintain appropriate records.The supplier shall observe the specifications of the board, device and soldering paste manufacturers, and provide evidence of compliance. The temperature profile shall therefore be recorded with the printed com-ponent circuit board.7.2 Proof of reliability of the assembly and connection technologyThe supplier shall document the development progress at the time of each delivery of sample parts.At the time of submission of the initial samples, the supplier shall perform a full qualification on the basis of the requirements of the KLH and provide the appropriate evidence.The supplier shall coordinate the number and scope of the tests with Development and Daimler AG Qual-ity Management and document the results.In order to allow the impact of changes on the component to be assessed, the supplier shall document a comparison of measuring results before and after the intended change.Qualification shall be carried out using components manufactured on the series production equipment. 7.3 Proof of reliability of the devices usedOn delivery, the supplier shall provide evidence of device qualification.For ICs, the supplier shall provide evidence of the device qualification in accordance with AEC-Q100, for discrete components in accordance with AEC-Q101, and for passive components in accordance with AEC-Q200.To achieve the zero-defects strategy, the supplier shall document the methods as per AEC-Q004 and provide evidence of the records to Daimler AG Quality Management.The supplier shall have any deviations from these specifications approved by Daimler AG Quality Man-agement.7.4 Board bending testThe supplier shall ensure that soldered circuit boards or devices cannot be damaged as a result of me-chanical stresses. Excessive mechanical stresses result in the danger of the board or devices becoming pre-damaged due to microcracks. The supplier shall support the PCB boards using an appropriate fixture.By means of a board bending test, the mechanical stress to which a soldered circuit board is exposed during the production process can be determined.The supplier shall perform a bending test for the following production steps on the component-specific board and maintain the relevant records:- Paste printer (only for double-sided boards)- SMD placement machines- ICTseparatorboard- Circuit- Press-fit process for contacts- Press-fit and assembly fixtures and jigs for installing boards in a housing- Transport systems, including gripping devices.The supplier shall repeat the board bending test at regular time intervals and record the relevant results.The supplier shall use the bending test for fault finding in the event of failures of devices (e.g. damage, microcracks on ceramic capacitors). The supplier shall record the results and submit them to Daimler AG Quality Management on request.The supplier shall use an appropriate measurement procedure for carrying out the board bending test.The maximum critical bending of boards depends on the individual circuit board or the devices used. The supplier shall take care to ensure that the sensors are positioned on the board at the point of maximum bending.The supplier shall take care to ensure that circuit board is assembled and soldered in line with the relevant process step to be examined.During the processing of ceramic capacitors, the supplier shall ensure that the specifications ofAV A2110039899 "Design Rules for E/E Components“ are complied with for all manufactured compo-nents.7.5 RequalificationThe supplier shall check at least once every year whether its deliveries conform to the specifications of Daimler AG.As a minimum requirement, the test scope shall include evidence that the specifications with regard to dimensional, material, reliability, environmental, process and statutory rules have been complied with.The supplier shall coordinate and document the test scopes with Daimler AG Quality Management. This coordination shall be based on the environmental and lifetime tests specified in the component require-ment specifications (KLH) as well as other specifications such as DBL, MBN, AV, etc.The supplier can choose between the following methods to prove compliance with the specifications of the environmental and life tests required in KLH:- complete annual repeat of the of the environmental and life tests specified in KLH- annual execution of a so-called "Q-Review Environment E/E“.7.5.1 Complete repeat of the environmental and life testsThe supplier shall record the results of the repeat and submit them to Daimler AG Quality Management on request.The supplier shall notify Daimler AG Quality Management of any deviations from the specification without delay.The supplier shall supply regular evidence of the process capabilities of production as a whole and each production process and maintain the appropriate records.If the tests show that the required cp or cpk values are not achieved and that the equipment requires read-justment, the supplier shall shorten the test interval.7.5.2 Q-Review Environment E/ETo perform a "Q-Review Environment E/E“, the supplier is required to comply with the following conditions: The environmental and life tests specified in the KLH have been performed once successfully, and the relevant results recorded.Another condition for the execution of a "Q-Review Environment E/E“ is that the following requirements have been fulfilled during the previous 12 months:- The supplier has used a statistical method for the early detection of faults in production. This method has ensured that 100% of the manufactured parts have been covered, the results recorded and evaluated regularly. All measures defined as part of the early fault detection system during the previous 12 months must have been effectively implemented.- The required qualification tests shall have been passed successfully with regard to any changes to the component or the production process.- All failures during the tests in production have been determined, and the relevant results recorded and regularly evaluated. All measures defined during the previous 12 months shall have been effectively im-plemented.- All measures defined during internal and external audits during the previous 12 months shall have been effectively implemented.- All 0-km failures and field failures during the previous 12 months shall have been analyzed and evalu-ated. Any resulting measures shall have been implemented effectively.。

最新体检表emgs

最新体检表emgs

HEALTH EXAMINATION GUIDELINESFOR ENTRY INTOMALAYSIAN HIGHER EDUCATIONAL INSTITUTIONS1. PLEASE READ THE INSTRUCTIONS CAREFULLY BEFORE FILLING IN THE FORM.2. PLEASE FILL IN THE FORM IN ENGLISH LANGUAGE.3. PLEASE WRITE IN CAPITAL LETTERS.4. THIS FORM HAS 4 SECTIONS:a) SECTION 1 (PART A AND B) TO BE FILLED BY THE APPLICANT; ANDb) SECTION 2,3 AND 4 TO BE FILLED BY THE EXAMINING DOCTOR5. PLEASE COMPLETE THE ENTIRE TEST REQUIRED IN THIS FORM.6. THE UNIVERSITY / COLLEGE ONLY ACCEPT MEDICAL EXAMINATION DONE WITHIN90 DAYS BEFORE ARRIVAL IN MALAYSIA.THEORIGINAL LABORATORY RESULTS.ALLATTACH7. PLEASEALONGCHEST X-RAY FILM (OR DIGITAL IMAGES) AND REPORT BRING8. PLEASEFOR REGISTRATION, FOR THE PURPOSE OF VERIFICATION, IF NECESSARY.9. PLEASE ENSURE THE X-RAY FILMS OR DIGITAL IMAGES ARE LABELLED WITHYOUR NAME AND DATE TAKEN (IN ENGLISH).10. CHEST X-RAY DONE WITHIN 6 MONTHS PRIOR TO REGISTRATION CAN BE ACCEPTED.11. THE UNIVERSITY / COLLEGE RESERVES THE RIGHT TO REPEAT FULL MEDICALCHECK UP OR ANY SPECIFIC LABORATORY TESTS SHOULD THERE BE ANY DOUBT IN THE MEDICAL REPORT SUBMITTED, ALL COSTS INVOLVED SHALL BE BORNE BY THE CANDIDATES.12. T H E U N I V E R S I T Y /C O L L E G E R E S E RV E S T H E R I G H T TO R E J E C T A N YAPPLICATION:a) BASED ON THE RESULTS OF THE HEALTH EXAMINATION; ORb) SHOULD THERE BE ANY EVIDENCE THAT THE APPLICANT HAS GIVENFALSE INFORMATION IN THE HEALTH EXAMINATION REPORT OR ANYDOCUMENTS.SUPPORTINGSECTION 1 (PART A)FULL NAME (AS IN PASSPORT)INTERNATIONAL PASSPORT NUMBER NATIONALITY CONTACT NUMBER IN MALAYSIABLOOD GROUP (RHESUS)ACADEMIC YEAR STUDENT ID PROGRAMME OF STUDYPROGRAMME CODEH T R I B F O E T A D AGE NEXT OF KINNEXT OF KIN’S ADDRESS NEXT OF KIN’S CONTACT NUMBER SEX MARITAL STATUSThe details of the blood type recorded here are as reported by the patient and have not been tested or verified to be correct by the medical practitioner completing this online medical screening questionnaire. The medical practitioner completing this form disclaims any and all liability to the fullest extent permitted by law for any personal injury, suffering or loss caused by any reliance on this information by any other party.SECTION 1 (PART B)Declaration of self and family illness. Explain in full if you or your immediate* family has any of the following illnesses. * Immediate family refers to mother, brothers / sisters.Current medication (Long Term)Notes :1. *A valid Yellow Fever vaccination certificate is required from all travellers coming from or transited more than 12 hours through countries with risk of Yellow Fever transmission.2.All students are required to take vaccines as listed in numbers 2-7 above.3.The students are required to bring along the International Certificate of Vaccination or Prophylaxis with them for verification of information.SECTION 2 - PHYSICAL EXAMINATION1. BASIC MEASUREMENTVISION TESTNORMAL DEFECTIVE FULL NAME (AS IN PASSPORT)INTERNATIONAL PASSPORT NUMBER TYPE OF APPLICATIONEMGS REFERENCE NUMBER DATE OF MEDICAL SCREENING WEIGHT (kg)SYSTOLIC (mmHg)DIASTOLIC (mmHg)UNAIDED (L)UNAIDED (R)HEIGHT (m) :BLOOD PRESSURE:PULSE RATE (PER MINUTE)BMI(kg/m²)AIDED (L)AIDED (R)COLOR VISION TEST COMMENT 2. GENERAL EXAMINATIONHEARING ABILITYNORMAL DEFECTIVE COMMENTLEFTRIGHTSECTION 2A - PHYSICAL EXAMINATION - EBOLAHave you in the last 30 days travelled to or from the following Ebola affected countries:FULL NAME (AS IN PASSPORT)INTERNATIONAL PASSPORT NUMBER TYPE OF APPLICATIONEMGS REFERENCE NUMBER DATE OF MEDICAL SCREENINGHave you in the last 30 days come into contact with someone, who has in the last 30 days, traveled to or from thefollowing Ebola affected countries:Do you have any of the following Ebola virus symptoms?Have you in the last 30 days come into contact with Ebola infected persons or animals?SECTION 3 - LABORATORY RESULTSFULL NAME (AS IN PASSPORT)INTERNATIONAL PASSPORT NUMBER EMGS REFERENCE NUMBER DATE OF LAB TEST NAME OF LAB* TPHA is done if VDRL is reactive** all test results / reports is valid for 6 monthsSECTION 4 - CHEST X-RAY FINDINGSFULL NAME (AS IN PASSPORT)INTERNATIONAL PASSPORT NUMBER EMGS REFERENCE NUMBERPLACE OF CHEST X-RAY DATE OF CHEST X-RAYCHEST X-RAY NO.COMMENTFULL NAME (AS IN PASSPORT)INTERNATIONAL PASSPORT NUMBER EMGS REFERENCE NUMBER TYPE OF APPLICATION DATE OF CERTIFICATIONCOMMENTQUALIFICATION OF EXAMINING DOCTOR NAME OF EXAMINING DOCTOR HOSPITAL/CLINIC REGISTRATION NUMBERSECTION 5 - CERTIFICATION BY THE EXAMINING DOCTORHEREBY THE STUDENT IS CERTIFIED ASFOR STUDY IN MALAYSIA.SUITABLE UNSUITABLE。

简式Token测验(36项)报告单

简式Token测验(36项)报告单
27、摸黑色圆形或者红色方形,
28、把绿色方形从黄色方形旁边拿开,
29、如果有蓝色圆形,摸红色方形,
30、把绿色方形放在红色圆形旁边,
31、慢慢地摸那些方形,很快地摸那些圆形,
32、把红色圆形放在黄色方形和绿色方形之间,
33、摸除了绿色之外的所有圆形,
34、摸红色圆形一-,不,白色方形,
35、摸黄色圆形,不是白色方形,
36、除了摸黄色圆形还要摸黑色圆形。
合计
最后总分
最后总分,—+_+—+_+—+—=.Jfr
检查医师
康复医学科
简式
姓名性别_年龄—门诊号住院号病区
病床文化程度(受教育年数)入院日期检查日期
、放20个代币(7分):
指令
年月日
年月日
年月日
1、摸一下圆形,
2-,摸一下方形,
3、摸一下黄的,
4、摸摸一下绿的,
7,摸一下白的,
合计
二、把小代币拿走:(4分)
指令
8,摸黄色的方形,
合计
五、把小代币放回:(4分)
指令
20、摸大的白色圆形和小的绿色方形,
21、摸小的黑色圆形和大的黄色方形,
22、摸大的绿色方形和大的红色方形,
23、摸大的白色方形和小的绿色圆形,
合计
六、把小代币拿走:(13分)
指令
24、把红色圆形放在绿色方形上,
25、用红色方形碰黑色圆形,
26、摸黑色圆形与红色方形,
9、摸黑色的圆形,
10、摸绿色的圆形,
11、摸白色的方形,
合计
三、把小代币放回:(4分)
指令
12、摸小的白色圆形,
13、摸大的黄色方形,

(完整版)SF-36中文问卷

(完整版)SF-36中文问卷

(完整版)SF-36中文问卷简介SF-36中文问卷是一种常用的用于评估个体健康状态和生活质量的工具。

它是基于美国麻省健康研究中心建立的SF-36问卷的中文翻译版本。

SF-36中文问卷包含了8个方面的问题,涵盖了身体健康、日常生活动作、身体疲劳、心理健康、社交功能、情绪问题等多个维度,能够全面评估个体的健康状况。

问卷内容1. 身体功能- 现在您因为身体问题,在过去四周里有多少时间不能正常工作或者研究?- 过去四周里,您因为身体健康问题,有多少时间感觉身体有限制,无法完成一些日常活动,比如走路或购物?- 过去四周里,您因身体状况不佳,有多少时间感到疲劳或精力不足?2. 生活质量- 现在您对自己的整体健康状况感到满意吗?- 过去四周里,您感到情绪低落或沮丧的时间有多长?- 过去四周里,您感到身心放松的时间有多长?3. 社交功能- 现在,您与他人的社交活动有多少限制?- 过去四周里,您因为身体或情绪问题,有多少时间无法正常与他人保持亲密关系?- 过去四周里,您因为身体或情绪问题,有多少时间感到孤独?4. 情绪问题- 现在,您感到焦虑或紧张的时间有多长?- 过去四周里,您感到情绪波动或不稳定的时间有多长?- 过去四周里,您因为身体或情绪问题,有多少时间感到沮丧或绝望?5. 总体健康评估- 现在,您对自己的整体健康状况评估如何?以上问题使用5个可能的回答选项,包括:非常多、有相当多、一些、很少、没有。

根据回答选项的不同,进行不同的评分,最终可以得出个体在各个维度上的健康状况评估。

使用方法使用本问卷时,请根据个体的实际情况和回答选项进行选择。

在选择问题答案时,请选择最符合个体状态的选项。

根据回答的情况,可以通过计算各个维度的得分,进而评估个体的健康状况和生活质量。

注意事项在使用本问卷时,应注意以下几点:- 本问卷仅供参考,不能代替专业医生的评估和诊断。

- 如有不明确的问题,请向专业医生进行咨询。

- 请根据回答选项之间的区别进行选择,不要选择多个选项。

Model Test Three 答案解析

Model Test Three 答案解析

Model Test Three答案解析Part I Listening ComprehensionSection A1.--- Mr. Smith, what do you think of the product?【解析】A。

本题考查对特殊疑问句的回答。

此题询问“史密斯先生,你觉得这个产品怎么样?”,回答应是对产品的看法,选项A(非常好)最符合题意,为正确答案。

2.--- Miss Green, would you please write your name here?【解析】D。

本题考查对请求的回答。

此题询问“格林小姐,你可以在这里签个字吗?”,选项D的意思是“好的,可以”表示同意的肯定回答,因此得知D为正确答案。

3.--- Hello, may I speak to Helen?【解析】A。

本题考查对电话用语的回答。

此题询问“您好,请帮我叫海伦接电话”,如果正是本人接电话则回答“yes, speaking”,非本人则一般回答“hold on, please”,因此得知A为正确答案。

4.--- How do you like your new job?【解析】C。

本题考查对特殊疑问句的回答。

此题询问“你觉得你的新工作怎么样?”,通常的回答应是对工作的评价(好或者不好),因此选项C(还行)为正确答案。

5.--- What’s wrong with Mike?【解析】A。

本题考查对特殊疑问句的回答。

此题询问“Mike怎么了?”,句型“what’s the wrong with----”一般用于询问某人,某事的情况,回答通常用具体描述的语句即可,选项A(并无大碍)最符合题意,为正确答案。

Section B6.M:Excuse me,Mr. Johnson asked me to come and see him this morning.W:Oh,yes,he is waiting for you in his office.Q:Where is Mr. Johnson now?【解析】B。

剑11-听力test-3-文本

剑11-听力test-3-文本

剑11-听力test-3-文本剑11听力test 3 文本Section 1Good morning. This is Bur nham tourist office, Martin speak ing.Oh, hello. I saw a poster about free things to do in the area, and it said people should phone you for in formatio n.「m comi ng to Bur nham with my husba nd and two childre n for a few days on June 27th, or possibly 28th, and rd like some ideas for things to do on 29th.Yes, of course. OK. The n let's start with a couple of eve nts especially for childre n.The art gallery is holdi ng an eve nt called 'Family Welcome' that day, when there are activities and trails to use throughout the gallery.That sounds in teresti ng. What time does it start?The gallery ope ns at 10, and the ' Family Welcome ' eve nt runs from 10.30 until 2 o'clock.The gallery stays ope n un til 5.And several times duri ng the day,they're going to show a short film that the gallery has produced.It dem on strates how ceramics are made, and there'll be equipme nt and materials for children to have a go themselves.Last time they ran the eve nt, there was a film about pain ti ng, which went dow nvery well with the childre n, and they're now work ing on one about sculpture.I like the sound of that. And what other eve nts happe n in Bur nham?Well, do you all enjoy liste ning to music? - Oh, yes.Well, there are several free con certs taking place at differe nt times one or two in the morning, the majority at lunchtime, and a couple in the eve ning.And they range from pop music to Latin American.The Latin American could be fun.What time is that?It's being repeated several times, i n differe nt places.They're performi ng in the cen tral library at 1 o' clock, the n at 4 it's in the City Museum,and in the eve ning, at 7.30, there's a Ion ger con cert, i n the theatre.Right. I 'll suggest that to the rest of thefamily.Somethi ng else you might be interested in is the boat race along the river.Oh, yes, do tell me about that.The race starts at Offord Mari na, to the north of Bur nham, and goes as far as Summer Pool.The best place to watch it from is Charlesworth Bridge, though that does get rather crowded.And who's tak ing part?Well, local boat clubs, but the sta ndard is very high.One of them came first in the West of En gla nd regi onal champi on ship in May this yearit was the first time a team fromBur nham has won.It means that n ext year they'll be represe nti ng the regi on in the n ati onal champi on ship.Now r ve heard someth ing aboutPaxt on Nature Reserve.It's a good place for spotting unusual birds, is n't it'?That's right ——throughout the year. There is a lake there as well as a river, and they provide a very attractive habitat.So it's a good idea to bring bino culars if you have them.And just at the mome nt you can see various flowers that are pretty unu sual the soil at Paxt on isn't very comm on. They're look ing good right now. Right. My husband will be particularly interested in that.And there's going to be a talk and slide show about mushrooms and you'll be able to go out and pick some afterwards and study the different varieties. Uhuh. And is it possible for childre n to swim in the river?Yes. Part of it has been fenced off tomake it safe for children to swim in.It's very shallow, and there's a lifeguard on duty whe never it's ope n. The lake is too deep, so swimmi ng isn't allowed there.OK, we must remember to bring their swimmi ng thin gs, i n case we go to Paxt on.How long does it take to get there by car from Bur nham?About 20 minu tes, but park ing is very limited,so it's usually much easier to go by bus —and it takes about the same time. Right. Well, I'll discuss the optio ns with the rest of the family. Thanks very much for all your help.You're welcome.Goodbye.Bye.Secti on 2 First of all, let me tha nk youall for com ing to this public meet ing, to discuss the future of our tow n.—Our first speaker is Shona Fergus on,— ___________________________________________________from Barford tow n coun cil. Shona. Tha nk you. First「II briefly give you some backgro und in formati on, then「II be ask ing you for your comme nts on developme nts in the town.Well, as you don't n eed me to tell you, Barford has cha nged a great deal in the last 50 years.These are some of the main cha nges. Fifty years ago, buses lin ked virtually every part of the tow n and the n eighbouri ng tow ns and villages. Most people used them freque ntly but not now because the bus compa nies concen trate on just the routes that attract most passe ngers.So parts of the tow n are no Ion ger served by buses.Eve n replac ing old un comfortablebuses with smart new ones has had little impact on passe nger n umbers. It's sometimes said that bus fares are too high, but in relati on to average in comes, fares are not much higher tha n they were 50 years ago. Chan ges in the road n etwork are affecti ng the tow n.The centre was rece ntly closed to traffic on a trial basis, making it much safer for pedestria ns.The impact of this is being measured. The new cycle paths, separat ing bikes from cars in most mai n roads, are being used far more tha n was expected, reduc ing traffic and improve air quality.And although the coun cil's attempts to have a bypass con structed have failed, we have n't give n up hope of persuadi ng the gover nment to cha nge its mind. Shopp ing in the tow n centre has cha nged over the years.Many of us can remember whe n the town was crowded with people going shopp ing.Numbers have been falling for several years, despite efforts to attract shoppers, for in sta nee by ope ning new car parks. Some people comb ine shopp ing with visits to the town's restaurants and cafes.Most shops are small in depe ndent stores, which is good,but many people prefer to use supermarkets and departme nt stores in n earby large tow ns as there are so few well-k nown cha in stores here. Turni ng now to medical facilities, the town is served by family doctors in several medical practices fewer tha n 50 years ago, but each catering for far more patients.Our hospital closed 15 years ago, which means jour neys to other towns are un avoidable.On the other hand, there are more den tists tha n there used to be. Employme nt patter ns have cha nged, along with almost everyth ing else. The nu mber of schools and colleges has in creased making that the main employme nt sector.Services, such as website desig n and acco untan cy, have grow n in importa nee,and surpris in gly, perhaps, manu facturi ng has n't see n the decline that has affected it in other parts of the coun try. Now r II very quickly outl ine curre nt plans for some of the town's facilities, before ask ing for your comme nts.As you'll know if you regularly use the car park at the railway stati on, it's usually full.The railway compa ny applied for permission to replace it with amulti-storey car park, but that wasrefused.In stead, the compa ny has bought some adjoi ning land and this will be used to in crease the nu mber of park ing spaces. The Grand the old cin ema in the high street will close at the end of the year and reope n on a differe nt site. You've probably see n the buildi ng un der con structi on.The pla n is to have three scree ns with fewer seats, rather tha n just the one large auditorium in the old cin ema.I expect many of you shop in the in door market.It's become more and more shabby- look ing, and because of fears about safety, it was threate ned with demoliti on.The good n ews is that it will close for six weeks to be made safe and redecorated and the improved buildi ng will ope n in July.Lots of people use the library,in cludi ng school and college stude nts who go there to study.The coun cil has man aged to secure funding to keep the library open later into the eve ning twice a week.We would like to enlarge the building in the not-too-dista nt future, but this is by no means defi nite.There's no limit on access to the n ature reserve on the edge of tow n, and this will continue to be the case. What will cha nge, though, is that the coun cil will no Ion ger be in charge of the area. In stead it will become theresp on sibility of a n ati onal body that admi nisters most n ature reserves in the coun try. OK, now let me ask you...Secti on 3Hello, Hele n. Sorry「m late.Hi, Jeremy, no problem. Well we'd bI _______________________________________________________________________________________etter work outwhere we are on our project, I suppose.Yeah. I've looked at the draw ings yo u've done for mystory, 'The Forest',and I thi nk they're brillia nt ——they r eally createthe atmosphere I had in mind when I was writing it.「m glad you like them.There are just a few suggesti ons「d like to make. Go ahead.Now, I'm not sure about the drawing of the cave —it's got trees all arou nd it, which is great, but the draw in g's a bit too static , is n't it?I thi nk it n eeds some acti on.Yes, there's nothing happe ning.Perhaps I should add the boy —Malc olm, is n't it?He would be walk ing up to it.Y es, let's have Malcolm in the drawing.And what about putt ing in a tiger——the one that he makes friends with a bit later?,but Maybe it could be sitting under a tre e washing itself.And the tiger stops in the middle of what it's doing whe n it sees Malcolm walk ing past.That's a good idea.OK, I'll have a go at that.Then there's the drawing of the cro wd of men and wome n dancing. They're just outside the forest, and t here's a lot going on.That's right, you wan ted them to be watch ing a carni val processi on I thought it would betoo crowded.Do you thi nk it works like this?Yes, I like what you've done.The only thing is, could you add Malc olm to it, without cha nging what's al ready there.What about hav ing him sitt ing on th e tree trunk on the right of the pictu re?Yes, that would be fine.And do you want him watch ing the o ther people? No, he's bee n left out of all the fun, so rd like him to be cryi ng that'll con trast ni cely with the nextpicture, where he's laughi ng at the c lowns in thecarni val .Right, I'll do that.And the n the draw ing of the people i ce skati ng in the forest.I was n't too happy with that one.Because they're supposed to be skat ing on grass, aren't they?That's right, and it's frozen over.At the mome nt it does n't look quite right.Mm, I see what you mean.「II have ano ther go at that.And I like the wool hats they're wea ring.Maybe you could give each of them a scarf as well.Yeah, that's easy eno ugh.They can be streami ng out beh ind th e people to suggest they're skati ng r eally fast.Mm, great. Well that's all on the dra win gs. Right. So you've finished writing you r storyand I just need to finish illustrating i t, and my storyand your draw ings a re done.So the n ext thing is to decide what exactly we n eed to write about in the report that goes with the stories, and how we're going to divide the work. Right, Hele n.What do you thi nk about in cludi ng a secti on on how we pla nned the project as a whole, Jeremy? That's probably quite importa nt.Yeah. Well, you've had most of the good ideas so far.How do you feel about draft ing somethi ng, the n we can go through it together and discuss it? OK, that seems reas on able.And I could in clude somethi ng on how we came up with the ideas for our two stories, could n't I? Well I've started writing something about that so why don't you do the same and we can in clude the two thin gs.Right. So what about our interpretation of the stories?Do we n eed to write about what we think they show,like the value of helping other people, all that sort of thing?That's gonna come up later isn't it?I thi nk every one in the class is going to read each other's storiesand come up with their ownin terpretati ons which we're going to discuss. Oh, I missed that.So it isn't going to be part of the report at all? No. But we n eed to write about the illustrati ons, because they're an esse ntial eleme nt of childre n's experie nee of readi ng the stories.It's probably easiest for you to write that secti on, as you know more about draw ing tha n I do. Maybe, but I find it quite hard to write about.rd be happier if you did it.OK. So when do you think...Section 4So what「m going to talk about to you today is somethi ng called Eth no graphy.This is a type of research aimed at____explori ng the way huma n cultures work.It was first developed for use in an thropology, and it's also bee n used in sociology and comm uni cati on studies.So what's it got to do with bus in ess, you may ask.Well, bus in esses are finding that eth no graphy can offer them deeper in sight into the possible n eeds of customers either prese nt or future as well as providi ng valuable in formati on about their attitudes towards existi ng products.And eth no graphy can also help compa nies to desig n new products or services that customers really want.Let's look at some examples of how eth no graphic research works in bus in ess.One team of researchers did a project for a company manufacturing kitchen equipme nt. They watched how cooks used measuri ng cups to measure out things like sugar and flour.They saw that the cooks had to check and recheck the conten ts, because although themeasuri ng cups had n umbers in side them the cooks could n't see these easily.So a new desig n of cup was developed to overcome this problem, and it was a top seller. Ano ther team of eth no graphic researchers looked at how cell pho nes were used in Ugan da, i n Africa.They found that people who did n't have their own pho nes could pay to use the pho nes of local en trepre neurs.Because these customers paid in adva nee for their calls, they were eager to know how much time they'd spe nt on the call so far.So the phone compa ny desig ned phones for use globally with this added feature.Eth no graphic research has also bee n carried out in computer compa ni es.In one compa ny, IT systems adm ini strators were observed for several weeks.It was found that a large amo unt of their work in volved comm uni cat ing with colleagues in order to solve problems,but that they did n't have a sta ndard way of excha nging in formati on from spreadsheets and so on. So the team came up with an idea for software that would help them to do this.In ano ther piece of research, a team observed and talked to nu rses work ing in hospitals.This led to the recog niti on that the nu rses n eeded to access the computer records of their patie nts no matter where they were.This led to the developme nt of a portable computer tablet that allowed the nu rses to check records in locati ons throughout the hospital. Occasi on ally, research can be done eve n in en vir onments where the researchers can't be prese nt.For example, i n one project done for an airli ne, resp ondents used their smart phones to record information during airline trips in a study aim ing at track ing the emoti ons of passe ngers duri ng a flight.So what makes studies like these differe nt from ordi nary research? Let's look at some of the general prin ciples beh ind eth no graphici _____________________________________________________research in bus in ess.First of all, the researcher has to be completely ope n-min ded he or she has n't thought up a hypothesis to be tested, as is the case in other types of research.In stead they wait for the participa nts in the research to inform them.As far as choos ing the participa nts themselves is con cer ned, that's not really all that differe nt from ordi nary researchthe criteria according to which the participa nts are chose n may be somethi ng as simple as the age bracket they fall in to, or the researchers may select them accordi ng to their in come, or they might try to find a set of people who all use a particular product, for example.But it's absolutely crucial to recruit the right people as participa nts.As well as the criteria I've men ti oned, they have to be comfortable talki ng about themselves and being watched as they go about their activities.Actually, most researchers say that people ope n up pretty easily, maybe because they're ofte n in their own home or workplace.So what makes this type of research special is that it's no t just a matter of sending a questi onn aire to the participa nts,in stead the research is usually based on first-ha nd observati on of what they are doing at the time. But that does n't mean that the researcher n ever talks to the participa nts.However, uni ike in traditi onal research, i n this case it's the participa nt rather tha n the researchers who decides what directi on the in terview will follow. This means that there's less likelihood of the researcher impos ing his or her own ideas on the participa nt.But after they've said goodbye to their participa nts and got back to their office, the researchers' work isn't fini shed.Most researchers estimate that 70 to 80 perce nt of their time is spe nt not on the collecting of data but on its an alysis look ing at photos, liste ning torecordi ngs and tran scrib ing them and so on. The researchers may end up with hun dreds of pages of no tes.And to determ ine what's sig nifica nt, they don't focus on the sen sati onal things or the unu sual thin gs, in stead they try to ide ntify a pattern of some sort in all this data, and to discer n the meaning behi nd it. This can result in some compelli ng in sights that can in turn feed back to the whole desig n process.。

圆二色测三级结构参数设置

圆二色测三级结构参数设置

圆二色测三级结构参数设置1.开氮气,并将流量计调到2-4L/min。

2.打开J-1500和计算机电源,开机。

3.点击SpectraManager桌面图标进入SpectraManager软件操作界面。

4.点击Spectra Measurement,开始仪器初始化,初始化结束后出现光谱扫面Spectra Measurement界面。

5.点击氙灯图标,倒计时结束后氙灯打开,氙灯预热15min后开始实验。

氙灯预热时可以点击Measure下的Parameter setting,进入测量参数的设定界面。

6.参数选择和设定General菜单下:Channels中选择测量模式。

Start(nm):扫描起始波长End :扫描结束波长。

Start mode :开始模式选择。

Scanning mode :扫描模式选择。

Scanning speed :扫描速度选择。

Sensitiveity :根据样品测量信号的振幅选择测量CD的灵敏度。

D.I.T : Digital Integration Time,响应时间选择。

Band width :标准操作时,谱带宽度选为1 nm。

Slit width : 狭缝选择,对于高分辨率测量,要用较窄的狭缝宽度。

Accumulation/Repeat :扫描次数设定。

Acquisition Duration:采点时间(0.05S~20S)。

Control选项里将shutter is opened and closed automatically选上。

7.样品的测量(1) 放入空白样品,点击Baseline Measurement进行基线测量。

测量结束后,把Parameter settings中control下的baseline选中,可以进行空白的扣除。

(2) 放入待测样品,点击Sample Measurement进行样品的测量。

(3) 固体粉末样品测试时,要尽可能地研磨获得细小均匀的样品,夹在石英片中间是用力要均匀,样品架旋紧即可,不可以用力过度,以免损伤石英片和样品架。

Testosterone ELISA 产品说明书

Testosterone ELISA 产品说明书

Testosterone ELISA Catalog Number SE120119 Storage Temperature 2–8 °CTECHNICAL BULLETINProduct DescriptionTestosterone (17β-hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group at C-3 and a hydroxyl group in the βposition at C-17. This steroid hormone has amolecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ∼50% of circulating testosterone is derived from peripheral conversion ofandrostenedione, ∼25% from the ovary, and ∼25% from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism andvirilization, polycystic ovaries, ovarian tumors, adrenal tumors, and adrenal hyperplasia. In male, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia, and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: hypopituitarism, Klinefelter’s syndrome, testicular feminization, orchidectomy and cryptorchidism, enzymatic defects, and some autoimmune diseases.The Testosterone ELISA kit is designed for the quantitative determination of total testosteroneconcentration in human serum or plasma. It is based on the principle of competitive binding betweentestosterone in the test specimen and testosterone-HRP conjugate for a constant amount of mouse anti-testosterone. In the incubation, mouseanti-testosterone coated wells are incubated with25 µL of testosterone standards, controls, samples, and 100 µL of testosterone-HRP conjugate reagent at room temperature for 60 minutes. During the incubation, a fixed amount of HRP-labeled testosterone competes with the endogenous testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific testosterone antibody.Thus, the amount of testosterone peroxidase conjugate immunologically bound to the well progressivelydecreases as the concentration of testosterone in the specimen increases. Unbound testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. ComponentsReagents and Equipment Required but Not Provided.1.Distilled or deionized water2.Precision pipettes. Disposable pipette tips3.ELISA reader capable of reading absorbance at450 nm4.Absorbent paper or paper towel5.Graph paperPrecautions and DisclaimerThis product is for R&D use only, not for drug,household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.2Preparation InstructionsSample Preparation1.Collect blood specimens and separate the serumimmediately.2.Specimens may be stored refrigerated at (2–8 °C)for 5 days. If storage time exceeds 5 days, storefrozen at (–20 °C) for up to one month.3.Avoid multiple freeze-thaw cycles.4.Prior to Assay, frozen sera should be completelythawed and mixed.5.Do not use grossly lipemic specimens.6.Samples containing sodium azide should not beused in the Assay.20× Enzyme ConjugatePrepare 1× working solution by diluting 20-fold with assay diluent as needed (e.g., 0.1 mL of the20× Enzyme Conjugate in 1.9 mL of Assay Diluent is sufficient for 20 wells). The diluted conjugate has to be used the same day.20x Wash Buffer ConcentratePrepare 1x wash buffer by adding the contents of the bottle to 475 mL of distilled water. Store 1x Wash buffer at room temperature.Storage/StabilityStore the kit at 2–8 °C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagents to heat, sun, or strong light.ProcedureNotes: The components in this kit are intended for use as an integral unit. The components of different lots should not be mixed.It is recommended that serum samples be run in duplicate.Optimal results will be obtained by strict adherence to this protocol. Accurate and precise pipetting, as well as following the exact time and temperature requirements prescribed are essential. Any deviation from this may yield invalid data.Before proceeding with the assay, bring all reagents to room temperature (18–26 °C).1.Pipette 25 µL of the standards, control, or specimeninto the assigned well.2.Add 100 µL of working Testosterone-enzymeconjugate reagent to all wells (see PreparationInstructions).3.Swirl the microplate gently for 20–30 seconds tomix the reagents.4.Cover the plate and incubate for 60 minutes atroom temperature.5.Remove liquid from all wells. Wash wells threetimes with 300 µL of 1x Wash buffer (seePreparation Instructions). Blot on absorbent papertowels.6.Add 100 µL of TMB Substrate reagent to all wells.7.Cover the plate and incubate at room temperaturefor 15 minutes.8.Add 50 µL of Stop Solution to each well and gentlymix for 15–20 seconds.9.Read the absorbance on ELISA Reader for eachwell at 450 nm within 15 minutes after adding theStop Solution.3Results1.Calculate the mean absorbance value (A450) foreach set of reference standards, controls, andsamples.2.Construct a standard curve by plotting the meanabsorbance obtained for each reference standardagainst its concentration in ng/mL on a linear-linear graph paper, with absorbance values on the vertical or Y axis and concentrations on the horizontal orX axis.e the mean absorbance values for eachspecimen to determine the correspondingconcentration of testosterone in ng /mL from thestandard curve.4.Any values obtained for diluted samples must befurther converted by applying the appropriatedilution factor in the calculations.Example of the standard curveExpected normal valuesIt is recommended that each laboratory establish its own normal ranges based on a representative sampling of the local population. The following testosterone ranges values were obtained from literature and may be used as initial guideline only:Males: prepubertal (late), 0.1–0.2 ng/mLAdult 3.0–10.0 ng/mLFemales: prepubertal (late), 0.1–0.2 ng/mLfollicular phase, 0.2–0.8 ng/mLluteal phase, 0.2–0.8 ng/mLpost-menopausal, 0.08–0.35 ng/mL References1.Chen, A. et al., Diagnosis of a testosterone-secreting adrenal adenoma by selective venouscatheterization. Fertil. Steril.,1991; 55: 1202-1203.2.Granoff,A.B., and Abraham, G.E., Peripheral andadrenal venous levels of steroids in a patient withvirilizing adrenal adenoma. Obstet. Gynecol., 1979;53:111-115.3.Bricaire, C. et al., Selective venous catheterizationin the evaluation of hyperandrogenism. J.Endocrinol Invest.,1991; 14: 949-956.4.Heinonen, P.K., Androgen production by epithelialovarian tumours in post-menopausal women.Maturitas,1991; 13: 117-117-1225.Tietz, N.W. ed., Clinical Guide to Laboratory Tests,3rd Edition, W.B. Saunders, Co., Philadelphia, 1995: 578-580.A Center for Disease Control/National Instituteof Health Manual, “Biosafety in Microbiological and Biomedical Laboratories"”847.ICN Guide to Endocrine Testing. DiagnosticDivision, ICN Biomedicals, Inc. pp. 2:33-35; 3:4-6.AI,CH,MAM 10/14-1©2014 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at and/or on the reverse side of the invoice or packing slip.。

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北京交通大学2009-2010学年第一学期大学英语II测验三(December 10, 2009)班级________ 姓名_________ 学号___________ 序号___Part One Fast Reading (15%)Directions: In this part, you will have to go over the passage quickly and answer the questions on the Answer Sheet.·For questions 1-7, choose the best answer on the basis of the choices given;·For questions 8-10, please complete the sentences with the information given in the passage.English spelling is confusing and chaotic, as any student of English knows all too well. "How can the letters ough spell so many different sounding words," they ask, "like dough, bough, rough, and through?" And what about a word like colonel that clearly contains no r, yet pretends it does, and ache with its k sound, instead of the chuh sound of arch? And why does four have a u while forty doesn't? There are no simple rules for English spelling, but there is an explanation behind its complexity. We have only to look back in history.Over the centuries, the English language has been like a magnet, attracting words from numerous languages. It all started with the Britons, an ancient people living in a part of Western Europe that eventually became the British Isles. They spoke a language called Celtic, which was a combination of the early forms of Irish, Scottish, and Welsh. When the Britons were conquered by the Romans and later the Germanics tribes, their language was also invaded. The merging of the languages gave birth to Old English (an early form of the Modern English we know), and a Latin alphabet replaced, with a few exceptions, the ancient Germanic alphabet. In the ninth century, the conquering Norsemen from Scandinavia added their pinch of language spice, as did the French in the eleventh century.By the fourteenth century, English, with its mix of at least five languages, had developed into what is called Middle English and had become Britain's official language. At that time, however, its spellings were far from consistent (一致的) or rational. Many dialects (方言) had developed over the centuries and sometimes people adopted the spelling used in one part of the country and the pronunciation used in another. For instance, today we use the western English spellings for busy and bury, but give the first the London pronunciation bizzy and the second the Kentish pronunciation berry. Of course, this all happened when English was primarily a spoken language, and only scholars knew how to read and write. Even they appeared to have been quite indifferent to matters of consistency in spelling, and were known to spell the same word several different ways in a single sentence.Even after William Caxton set up English's first printing press in the late fifteenth century, and the written word became available to everyone, standard spelling wasn't considered very important. As a matter of fact, the typesetters (排字工人) in the 1500s made things even worse by being careless about spelling. If a blank space needed to be filled or a line was too long, they simply changed the spellings of words to make them fit. Moreover, many of the early printers in England were fromGermany or Holland and didn't know English very well. If they didn't know the spelling of a word, they made up one! Different printers each had their favorite spellings, so that one word might be spelled five or six different ways, depending on who printed the passage.Throughout this period, names and words appear in many different forms. For instance, where can be found as wher, whair, wair, wheare, were, and so on. People were even very liberal about their names. More than eighty spellings of Shakespeare's name have been found, among them Shagspeare, Shaspeare, and even Shakestaffe. Shakespeare himself didn't spell his name the same way in any two of his six known signatures -- he even spelled his name two different ways in his will.By the late sixteenth century and early seventeenth century, some progress had been made in standardizing spelling due to the work of various scholars. By then, however, English spelling was far from a simple phonetic (语音的) system. For one thing, word pronunciations had changed too rapidly for a truly phonetic spelling to keep up. Also, English had borrowed from many languages and ended up having far too many sounds (more than forty) for the twenty-six letters in its Roman alphabet. By the time printing houses finally began to agree on standard spellings, many of these written forms were only a shadow of their spoken selves. In other words, spelling and pronunciation sometimes had little in common.Finally in 1775, Samuel Johnson gave English its first great dictionary. His choice of spellings may not have always been the best or the easiest, but the book helped to make the spellings of most English words uniform (一贯的). Eventually, people became aware of the need for "correct" spelling. Meanwhile, on the other side of the Atlantic, Noah Webster was standardizing American English in his American Dictionary of the English Language and American Spelling Book. Although the British had been complained about the messiness of English spelling for some time, it was the Americans who screamed the loudest. Webster not only favored a simplified, more phonetic spelling system, but also tried to persuade Congress to pass a law making the use of nonstandard spelling a punishable offense.In 1906, the philanthropist (慈善家) Andrew Carnegie gave $ 250,000 to help establish the Simplified Spelling Board (委员会). The board quickly issued a list of 300 words that were commonly spelled two ways, such as ax and axe, and called for the simpler of the two. The board helped to gain acceptance for quite a few American spellings, including catalog, dialog, and program.The call for simplified spelling quickly went out of fashion, particularly with the start of World War I and the death of Andrew Carnegie. The movement never died out completely, however. Spelling reform continued to be an ongoing, if less dramatic, progress, as it had been for centuries. Without the benefit of large donations or outside agencies, many words have dropped useless letters. “Deposite”had lost its e, as has “fossile”. “Musick” and “physick” have dropped their needless “k”s.As long as the world goes around, language will continue to change. New words will be added; spellings will be altered. But because people are most comfortable with the familiar, it's not likely that we'll ever see a major change in the way most words are spelled. Anyway, what would we do without the challenge of English spelling?1. In this article the author intends to ______________________________________.A) tell us how confusing and complex English spelling isB) give us some general idea about the development of the English spellingC) offer us an authoritative explanation for the complexity of English spellingD) encourage us to rise to the challenge of English spelling2. Which of the following is NOT true according to the passage?A) Celtic, a combination of earlier forms of Irish, Scottish and Walsh, is generally known as "OldEnglish".B) In ancient times, the Britons -- the natives of the British Isles -- were invaded by one alienculture after another.C) "Middle English" -- English with its mix of several other languages -- had become Britain'sofficial language by the fourteenth century.D) Before the invention of the printing press, English was mostly a spoken language.3. We may safely infer from the passage that _____________________________________.A) English was a relatively "pure" language before the fourteenth centuryB) conquering tribes forced the Britons to speak their languagesC) the influence of other languages made English a rich but complicated languageD) when Britain made English its official language, it stopped foreign words from entering thelanguage and making English even more complicated4. What is the main point of Paragraph 3?A) In Britain, English words had different spellings and pronunciations in different parts of thecountry.B) By the time English had become a written language, the influence of several languages anddialects had made spelling and pronunciation very inconsistent.C) Scholars did not help the problem of spelling inconsistency because they often spelled wordsseveral different ways.D) By the fourteenth century, English had developed into Middle English and was Britain's officiallanguage.5. In Paragraph 6, the author is mostly talking about __________________________________.A) how progress was made in standardizing spelling by the seventeenth centuryB) why English spelling and pronunciation were often very differentC) how English had many more sounds than it had letters in its alphabetD) why printing houses played a role in standardizing spelling6. It can be concluded from this passage ______________________________________.A) before the eighteenth century scholars were not much more educated than the massesB) even educated people were not much concerned with the spelling of words until the firstdictionary was writtenC) the invention of the printing press did have a significant influence on the English languageD) there was no real need for an English dictionary before Samuel Johnson wrote his in 17557. It can be inferred from this passage that ____________________________________.A) Samuel Johnson made the best and easiest choice of spellings in his dictionary, which helpedto make the spellings of most English words uniformB) Andrew Carnegie never played a significant role in the area of American English spellingC) spelling reform associations had less influence on English spelling changes than the naturalcourse of language changes todayD) thanks to many concerned people, spelling is simpler now than it was 200 years ago8. By the late 16th century and early 17th century, _____________________________________________________, since word pronunciations had changed too rapidly, and English had borrowedfrom many languages thus having too many sounds.9. ___________________________________ was responsible for giving English its great dictionary.10. We are not likely to see major changes in the way words are spelled because ________________.Part Two Listening Comprehension (100%)Section A (45%)Directions: In this section, you’ll hear 8 short conversations and 2 long conversations. At the end of each conversation, one or more questions will be asked about what was said. Both the conversation and the questions will be spoken only once. After each question there will be a pause. During the pause, you must read the four choices marked A), B), C) and D), and decide which is the best answer.11. A) Get out of the car. B) Pay the parking fine.C) T ake his coat off. D) Make a right turn.12. A) Seventeen pence. B) Seventeen percent of the bill.C) Seventy pence. D) Seven percent of the bill.13.A) Before dinner. B) During dinner. C) Right after dinner. D) The next day.14.A) In a courthouse. B) In a warehouse.C) In a department store. D) In a fashion designer‟s studio.15. A) She rearranged her artwork a while ago.B) Most people frequently change their minds.C) She sometimes considers changing majors.D) Everyone‟s inside for a short time.16. A) She went shopping yesterday. B) She doesn‟t like to go shopping.C) She doesn‟t live near the shops.D) She prefers shopping to studying.17. A) B ecause it‟s too late now to call Helen.B) Because Helen doesn‟t get up until six o‟clock.C) Because she has a bad memory.D) Because she wanted to call Helen before the rates went up.18. A) 10:45. B) 10:40. C) 10:55. D) 11:00.Questions 19 to 22 are based on the conversation you have just heard.19. A) Because it was boring. B) Because it was violent.C) Because the acting was not good. D) Because the scenery was bad.20. A) Good. B) Superb. C) Breathtaking. D) Excellent.21. A) Because he was giving his attention to the characters.B) Because he was busy appreciating the plot.C) Because he was upset by the violence.D) Because he felt sleepy.22. A) Two cowboys traced their cows. B) Two cobs who sold drug.C) Two policemen pursued the criminals.D) Two drug dealers being caught by the police.Questions 23 to 25 are based on the conversation you have just heard.23. A) Typing letters outside marketing. B) Typing records of meetings.C) Giving out the letters. D) Extra work she is expected to do.24. A) Rarely. B) Twice a day. C) Occasionally. D) Once a day.25. A) Because she likes it.B) Because it‟s usually related to marketing.C) Because someone else helps her with it.D) Because her boss is nice to her.Section B (30%)Directions: In this section, you will hear 3 short passages. At the end of each passage, you will hear some questions. Both the passage and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A), B), C) and D) Then mark the corresponding letter on Answer Sheet 2 with a single line through the centre.Passage OneQuestions 26 to 28 are based on the passage you have just heard.26. A) They told the newspaper about the matter. B) They told the public about the matter.C) They kept it secret. D) They told a few people.27. A) The signals were coming from the moon.B) The signals were coming from the earth.C) The signals were coming from other planets.D) The signals were coming from the Mars.28. A) The signals came from a “pulsar”(脉冲星). B) No answer came from a “pulsar”.C) Only one pulsar exists. D) Something like a “pulsar” exists.Passage TwoQuestions 29 to 31 are based on the passage you have just heard.29. A) 5 trillion cigarettes have been consumed last year.B) Chinese smoke heavily than those in the west.C) Cigarettes give many people a good deal of pleasure.D) Every person smoked 1,200 cigarettes per year.30. A) Impose a great deal of fine upon them. B) Put them into prison for years.C) Drive them away from the country. D) Pouring lead down their throat.31. A) A tranquilizing(镇静的) effect.B) A thrilling (令人激动的) effect.C) A fascinating effect. D) A surprising effect.Passage Three News32. Which of the following is Not mentioned in the report by a committee of education experts?A) Many American students cannot write well.B) Concerns of business leaders and teachers.C) More students have to pass a writing test before they can finish high school.D) All college entrance tests are changing now to include a writing test.33. What is the problem of teaching students to write well?A) Many students hate writing essays.B) Teachers do not have effective methods of teaching writing.C) It costs a large amount of time to read through written work.D) Computer programs have been developed to teach writing.34. Which of the following tests has already used e-readers?A) GMAT B) GRE C) TOEFL D) EPT35. How do teachers feel about computer grading?A) The systems can compare new essays against those already examined.B) The systems cannot really understand what a writer is trying to say.C) Computer grading guarantees that each piece of writing is graded in the same way.D) It is meant to judge knowledge more than creativity.Section C (25%)Directions:In this section, you will hear a passage three times. When the passage is read for the first time, you should listen carefully for its general idea. When the passage is read for the second time, you are required to fill in the blanks numbered from 36 to 43 with the exact words you have just heard. For blanks numbered from 44 to 46 you are required to fill in the missing information. For these blanks, you can either use the exact words you have just heard or write down the main points in your own words. Finally, when the passage is read for the third time, you should check what you have written.People travelling long distances frequently have to decide whether they would prefer to go by land, sea or air. Hardly anyone can (36) ___________ enjoy sitting in a train for more than a few hours. Train compartments soon get crowded and stuffy. It is almost impossible to take your mind off the journey. Reading is only a partial (37) ___________. During the day, sleep comes in snatches. At night, when you really wish to go to sleep, you rarely (38) ___________ to do so. Inevitably, you arrive at your (39) ___________ almost (40) ___________. Long car journeys are even less pleasant, for it is quite impossible even to read. On motorways you can, at least, travel safely at high speeds, but the greater part of the journey is spent on narrow, bumpy roads that are crowded with traffic.By (41) ___________, trips by sea offer a great variety of civilized (42) ___________. You can (43) ___________ your leg on the spacious decks, play games, swim, meet interesting people and enjoy good food. (44) ____________________________________________________________________. Even if you travel in ideal weather, sea journeys take a long time. Airplanes have the reputation of being dangerous. (45) ______________________________________________________________ ________________________________________________________________________________. Travelling at a height of 30,000 feet, far above the clouds, and at over 500 miles an hour is really unimaginable experience. For a few hours, you settle back in a deep armchair to enjoy the flight. An airplane offers you an unusual and breathtaking view of the world. If the landscape is hidden from view, you can enjoy the extraordinary sight of unbroken cloud plains that stretch out for miles before you, while the sun shines brilliantly in a clear sky. The journey is usually so smooth that there is nothing to prevent you from reading or sleeping. (46) _______________________________________________ ________________________________________________________________________________.Part Two Reading Comprehension (20%)Directions: There are 2 passages in this part. Each passage is followed by some questions. For each of them there are four choices marked A, B, C and D. You should decide on the best answer and mark the corresponding letter on the Answer Sheet with a single line through the center.Passage OneMany a young person tells me he wants to be a writer. I always encourage such people, but I also exp lain that there‟s a big difference between “being a writer”, and writing. In most cases these individuals are dreaming of wealth and fame, not the long hours alone at a typewriter. “You‟ve got to want to write,” I say to them, “not want to be a writer.”The reality is that writing is a lonely, private and poor-paying affair. For every writer kissed by fortune, there are thousands more whose longing is never rewarded. When I left a 20-year career in the U.S. Coast Guard to become a freelance writer, I had no prospects at all. What I did have was a friend who found me a place in a New York apartment building. It didn‟t even matter that it was cold and had no bathroom. I immediately bought a used manual typewriter and felt like a genuine writer.After a year or so, however, I still hadn‟t gotten a break and began to doubt myself. It was so hard to sell a story that barely made enough to eat. But I knew I wanted to write. I had dreamed about it for years. I wasn‟t going to be one of those people who die won dering – what if I would keep putting my dream to the last even though it meant living with uncertainty and fear of failure. This is the Shadowland of hope, and anyone with a dream must learn to live there.47. The passage is meant to _____.A) warn young people of the hardships that a successful writer has to experienceB) advise young people to give up their idea of becoming a professional writerC) show young people it‟s unrealistic for a writer to pursue wealth and fameD) encourage young people to pursue a writing career48. What can be concluded from the passage?A) Genuine writers often find their work interesting and rewarding.B) A writer‟s success depends on luck rather than on effort.C) Famous writers usually live in poverty and isolation.D) The chances for a writer to become successful are small.49. Why did the author begin to doubt himself after the first year of his writing career?A) He wasn‟t able to produce a single book. B) He hadn‟t seen a change for the better.C) He wasn‟t able to have a rest for a whole year.D) He found his dream would never come true.50. “...people who die wondering, w hat if” ( Para. 3) refers to “those ______”.A) who think too much of the dark side of life B) who regret giving up their career halfwayC) who think a lot without making a decision D) who are full of imagination even upon death51. “Shadowland” in the last sentence refers to _____.A) the wonderland one often dreams aboutB) the bright future that one is looking forward toC) the state of uncertainty before one‟s final goal is reachedD) a world that exists only in one‟s imaginationPassage TwoBotany (植物学), the study of plants, takes up a peculiar position in the history of human knowledge. For many thousands of years it was the one field of awareness about which humans had anything more than the vaguest of insights. It is impossible to know today just what our Stone Age ancestors knew about plants, but from what we can observe of preindustrial societies that exist, a detailed learning of plants and their properties must be extremely ancient. This is logical. Plants are the basis of the food pyramid for all living things, even for other plants. They have always been enormously important to the welfare of peoples, not only for food, but also for clothing, weapons, tool, dyes, medicines, shelter, and a great many other purposes. Tribes living today in the jungles of the Amazon recognize literally hundreds of plants and know many properties of each. To them, botany, as such, has no name and is probably not even recognized as a special branch of “knowledge” at all.Unfortunately, the more industrialized we become the father away we move from direct contact with plants, and the less distinct our knowledge of botany grows. Yet everyone comes unconsciously on amazing amount of botanical knowledge, and few people will fail to recognize a rose or an apple. When our Neolithic (新石器时代) ancestors, living in the Middle East about 10,000 years ago, discovered that certain grasses could be harvested and their seeds planted for richer yields the next season, the first great step in the new association of plants and humans was taken. Grains were discovered and from them flowed the marvel of agriculture: cultivated corps. From then on, humans would increasingly take their living from the controlled production of a few plants, rather than getting a little here and a little there from many varieties that grew wild, and the accumulated knowledge of tens of thousands of years of experience and intimacy (亲密) with plants in the wild would begin to fade away.52. Which of the following assumption about early humans is expressed in the passage?A) They probably had extensive knowledge of plants.B) They refused to accept the study of botany.C) They thought there was no need to cultivate crops.D) They placed great importance on the ownership of property.53. The author believes that the general knowledge of botany begun to fade is because_______.A) people fail to keep up with the fast evolution of plantsB) people no longer value plants as useful resourcesC) direct contact with a variety of plants decreasedD) our study of plants advances a higher stage54. What was the first step towards the practice of agriculture?A) The discovery of grasses that could be harvested and replanted.B) The system of botany had been formed.C) The learning of plants and their properties could not satisfy people‟s need for a better living.D) In the long-term study of botany, people came to realize the importance of agriculture.55. The word “marvel” in Par a. 2 most probably means “________”.A) march B) miracle C) threat D) aid56. According to the passage, which of the following statement is true?A) Tribes living in the jungles of the Amazon regard botany as an enormously important branch ofknowledge.B) Since plants are the basis of the food chain in the ecological system, it is natural that ourancestors had had a detailed study of plants and their properties.C) The more industrialized we become, the less direct contact we have with plants, which results inthe fact that we have little chance to learn botanical knowledge.D) After the practice of agriculture had been started, humans stopped passing down theaccumulated knowledge of botany.使用交互英语课本同学请做下面的题Part Four Vocabulary (30%)Section A (15%)Directions: Fill in the blanks in the following sentences with the words given below. Change the form where necessary.57. The __________ team must in turn try to keep the ball in the air before hitting it back over the net.58. The cost of the alcohol duty _________ according to the amount of wine in the bottle.59. The purpose of New Economic Policy is to ________an open, fair and equitable marketenvironment.60. The rabbit sat without moving, ___________ by the glare of our headlights.61. The nurse ____________ the rug around the sick man‟s legs carefully to keep him warm.62. We had been waiting for the design samples for over a week, but at last we were informed that thedesign samples were sent to the wrong ____________.63. Why do people purchase with credit cards? Possibly credit cards create the __________ that youcan own things without paying for them.64. People like to read the book introducing the magic __________ that will transform sorrow intohappiness.65. It‟s important for you to list your tasks in order of __________ when you work on a project nexttime.66. Since fixed salary is changed into performance-__________ pay, the factory has promoted theenthusiasm of the employees and its output has been tripled these years.Section B (15%)Directions: Complete each of the following sentences with the proper form of the word given in brackets.67. _____________ of these proposals could have disastrous consequences.( implement)68. France has _________ creating a rapid-reaction force to deal with the crisis. (propose)69. People have been _________ of the way she handled the affair. (critic)70. We tried to stay away from _____________ topics at the dinner party. (controversy)71. Recently there has been a __________ of interest in environmental issues. (renew)72. Because of the high exchange rate, our products have lost their __________ edge. (compete)73. That is, certain species may ___________ and others may survive for no particular reason.(elimination)74. The workmen are very _____________, so the work goes on smoothly.(cooperate)75. Dennis always seeks ___________ in his girlfriends. (perfect)76. It is so hot that my clothes are wet with ___________. (perspire)Part Five Banked Cloze (10%)Directions: Select one word for each blank from the list of choices given in the word bank.When 77) _________afterwards, Jody-Anne said that there were several words in the bee that she did not know how to spell, but that “God gave me the words that I knew.” Her advice to the other spellers? “Trust God, work hard, and never lose 78) _________of your goal.”Coach Archer agrees. ”We believe that inspiration plus 79) _________ is a 80) _________ formula,” he says.When Jody-Anne came home, she got a 81) _________ welcome. The government of Jamaica 82)_________ her via a billboard 83) _________ on the grounds of the 84) _________ minister‟s home. It also promised to pay for the rest of her 85) _________ at Ardenne High School in Kingston. The University of Jamaica gave her a full college scholarship to study anything she likes. Other universities in the U.S. have talked about giving her a scholarship to study 86) ________ law in graduate school, too. She has been thinking about becoming a lawyer for a couple of years.Part Six Translation (15%)Directions: Translate the following sentences into Chinese or vice versa.87. Because Jody-Anne was so busy after the spelling bee, her school excused the straight-A studentfrom her final exams. It doesn‟t get much better than that!88. Students who speak a language other than English are allowed to take one year of ESL.89. ___________________________________ (持之以恒的学习终于得到回报). About 3000 to 4000kids competed in the Jamaican national spelling bee in August 1997. And Jody-Anne won.90. __________________________________________________ (实施双语教育的方法) by thedifferent states vary greatly.91. ____________________________________________________________ (许多学生被压得喘不过气来) during the first two years of immersion bilingual education.。

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