Enhancing optimal feeder assignment of the multi-head surface mounting machine using genetic algorit
开始疯狂写作业英语
Certainly!Heres a detailed account of what one might experience while starting to tackle a pile of English homework assignments:1.Setting Up the Workspace:Before diving into the assignments,its important to create a conducive environment.This includes a clean desk,necessary stationery,and a comfortable chair to ensure long hours of focused work.anizing the Assignments:To avoid feeling overwhelmed,its helpful to organize the assignments by due date or subject.This allows for a systematic approach,starting with the most urgent or challenging tasks.3.Understanding the Requirements:Each assignment has specific requirements that need to be understood thoroughly.This involves reading the instructions carefully and noting any key points or questions that need to be addressed.4.Researching:For assignments that require research,such as essays or reports,the first step is to gather relevant information from textbooks,online resources,and academic databases.Its crucial to keep track of sources for proper citation.5.Drafting an Outline:Before writing,its beneficial to create an outline.This serves as a roadmap for the assignment,helping to organize thoughts and ensure that all necessary points are covered.6.Writing the Assignment:With a clear plan in place,the actual writing process can begin.This involves drafting the introduction,body,and conclusion of the assignment.Its important to use clear,concise language and to develop strong arguments supported by evidence.7.Editing and Proofreading:After the first draft is complete,its time to review and refine the work.This includes checking for grammatical errors,ensuring proper sentence structure,and verifying that the content flows logically.8.Incorporating Feedback:If the assignment allows for peer review or teacher feedback, its important to incorporate this into the final draft.Constructive criticism can help improve the quality of the work.9.Formatting and Citing:Depending on the assignment,there may be specific formatting requirements,such as MLA,APA,or Chicago style.Its essential to adhere to these guidelines and to cite all sources correctly to avoid plagiarism.10.Submitting the Assignment:Once the assignment is polished and meets all requirements,its ready for submission.This may involve uploading it to an online portal or handing it in physically.11.Reflecting on the Process:After submission,its helpful to reflect on the process. What worked well?What could be improved for next time?This reflection can lead to better strategies for future assignments.Remember,the key to successfully completing English homework is to break down the task into manageable parts,stay organized,and give yourself enough time to research, write,and revise.。
美国商务部管制清单索引
ALPHABETICAL INDEXTO THE COMMERCE CONTROL LISTThis index is not an exhaustive list of controlled items.Description ECCN Citation Ablative liners, thrust or combustion chambers .................................................................. 9A106.a Abrin ................................................................................................................................. 1C351.d.1 Absettarov (Central European tick-borne encephalitis virus) ..................................... 1C360.a.1.a.1 Absolute reflectance measurement equipment .................................................................... 6B004.a Absorbers of electromagnetic waves ...................................................................................... 1C001 Absorbers, hair type ...............................................................................................1C001.a Note 1.a Absorbers, non-planar & planar ....................................................................... 1C001.a Note 1.b&c Absorption columns ............................................................................................................. 2B350.e Accelerators (electro-magnetic radiation) ............................................................................ 3A101.b Accelerators or coprocessors, graphics .............................................................................. 4A003.d Accelerometer axis align stations .............................................................................. 7B003, 7B101 Accelerometer test station .......................................................................................... 7B003, 7B101 Accelerometers & components therefor ................................................................ 7A101 Accelerometers & accelerometer components ........................................................................ 7A001 Acoustic beacons ........................................................................................................... 6A001.a.1.b Acoustic beam forming software ...................................................................................... 6D003.a.1 Acoustic hydrophone arrays, towed ............................................................................. 6A001.a.2.b Acoustic location & object detection systems ............................................................... 6A001.a.1.b Acoustic, marine, terrestrial equipment .................................................................................. 6A991 Acoustic mounts, noise reduction equipment for vessels .............................................. 8A002.o.3.a Acoustic-optic signal processing devices ......................................................................... 3A001.c.3 Acoustic positioning systems ......................................................................................... 6A001.a.1.d Acoustic projectors ........................................................................................................ 6A001.a.1.c Acoustic seabed survey equipment ................................................................................ 6A001.a.1.a Acoustic systems, diver deterrent ....................................................................................... 8A002.r Acoustic systems, marine ..................................................................................................... 6A001.a Acoustic transducers ...................................................................................................... 6A001.a.2.c Acoustic underwater communications systems ............................................................... 5A001.b.1 Acoustic vibration test equipment .......................................................................................... 9B006 Acoustic wave devices ......................................................................................................... 3A001.c Acoustic-wave device manufacturing equipment and systems .................................. 3B991.b Note Active compensating system rotor clearance control software ............................................ 9D004.d Active flight control system software .................................................................................. 7D003.e Active flight control system technology .............................................................................. 7E004.b Active magnetic bearing systems......................................................................................... 2A001.c Active acoustic systems .................................................................................................... 6A001.a.1 Export Administration Regulations Bureau of Industry and Security January 9, 2012Actively cooled mirrors .................................................................................................... 6A005.e.1 Adaptive control software .................................................................................................... 2D992.a Adaptive control software ................................................................................................. 2D002.b.2 ADCs (analog-to-digital converters).................................................................................... 3A101.a ADCs (analog-to-digital converters)................................................................................. 3A001.a.5 ADCs (analog-to-digital converters).................................................................................... 4A003.e Aero gas turbine engine/assemblies/component test software............................................. 9D004.b Aero gas turbine engines ......................................................................................................... 9A001 Aerodynamic isotope separation plant/element housings ................................................. 0B001.a.3 Aerodynamic separation process systems & components ................................................... 0B001.d Aerosol challenge testing chambers ..................................................................................... 2B352.g Aerosol generating units specially designed for fitting to the systems specified in 2B352.h.1 or h.2 ...................................................................................... 2B352.h.3 Aflatoxins .......................................................................................................................... 1C351.d.2 African horse sickness virus ........................................................................................... 1C352.a.17 African swine fever virus (animal pathogens) .................................................................. 1C352.a.1 Agitators (chemical manufacturing) .................................................................................... 2B350.b AHRS (Attitude Heading Reference Systems), source code .................................................. 7D002 Air traffic control software ............................................................................................... 6D003.h.1 Air independent power systems (for underwater use) ..........................................................8A002.j Air compressors and filtration systems designed for filling air cylinders ............................8A992.l Air Traffic Control software application programs ................................................................ 6D993 Airtight vaults ......................................................................................................................... 0A981 Airborne altimeters ................................................................................................................. 7A006 Airborne altimeters ................................................................................................................. 7A106 Airborne communication equipment ...................................................................................... 7A994 Airborne radar equipment ....................................................................................................... 6A998 Aircraft .................................................................................................................................... 7A994 Aircraft, civil ........................................................................................................................ 9A991.b Aircraft, demilitarized .......................................................................................................... 9A991.a Aircraft, n.e.s........................................................................................................................... 9A991 Aircraft, trainer ....................................................................................................................... 9A018 Aircraft breathing equipment and parts ............................................................................... 9A991.e Aircraft (military) pressurized breathing equipment ........................................................... 9A018.d Aircraft inertial navigation systems & equipment ............................................................... 7A103.a Aircraft inertial navigation systems & equipment .................................................................. 7A003 Aircraft parts and components ............................................................................................. 9A991.d Akabane virus ................................................................................................................ 1C360.b.1.a Alexandrite ........................................................................................................................... 6C005.b Alexandrite lasers........................................................................................................... 6A005.c.2.b Align & expose step & repeat equipment (wafer processing) ..........................................3B001.f.1 Alignment equipment for equipment controlled by 7A .......................................................... 7B001 Export Administration Regulations Bureau of Industry and Security January 9, 2012Description ECCN Citation Alkylphenylene ethers or thio-ethers, as lubricating fluids .............................................. 1C006.b.1 Alloy strips, magnetic .......................................................................................................... 1C003.c Alloyed materials production systems and components ......................................................... 1B002 Alloyed metal materials in powder or particulate form ....................................................... 1C002.b Alloyed metal materials in the form of uncomminuted flakes, ribbons, or thin rods .......... 1C002.c Alloys, aluminum ................................................................................................................. 1C202.a Alloys, aluminum ........................................................................................................... 1C002.a.2.d Alloys, magnesium ........................................................................................................ 1C002.a.2.e Alloys, metal powder or particulate form ............................................................................ 1C002.b Alloys, nickel ............................................................................................................... 1C002.a.2.a Alloys, niobium.............................................................................................................. 1C002.a.2.b Alloys, titanium.................................................................................................................... 1C202.b Alpha-emitting radionuclides, compounds, mixtures, products or devices ............................ 1C236 Altimeters, airborne ................................................................................................................ 7A006 Altimeters, radar or laser types ............................................................................................. 7A106 Alumina fibers ......................................................................................................... 1C010.c Note 1 Aluminides ........................................................................................................................ 1C002.a.1 Aluminides, nickel ......................................................................................................... 1C002.a.1.a Aluminides, titanium...................................................................................................... 1C002.a.1.b Aluminum alloys ............................................................................................................ 1C002.a.2.d Aluminum alloy/powder or particulate form ............................................................... 1C002.b.1.d Aluminum alloys as tubes/solid forms/forgings ................................................................. 1C202.a Aluminum electroplating equipment .................................................................................... 2B999.i Aluminum gallium nitride (AlGaN) “substrates”, i ngots, boules, or otherpreforms of those materials ................................................................................................. 3C005 Aluminum nitride (AlN) “substrates”, ingots, boules, or other preforms ofthose materials ..................................................................................................................... 3C005 Aluminum organo-metallic compounds ................................................................................. 3C003 Aluminum oxide powder, fine ................................................................................................ 0C201 Aluminum powder, spherical .......................................................................................... 1C111.a.1 Aluminum powder (spherical) production equipment ............................................................ 1B102 Amalgam electrolysis cells, lithium isotope separation .................................................. 1B233.b.3 Amalgam pumps, lithium and/or mercury ........................................................................ 1B233.b.2 Ammonia, aqueous ................................................................................................................. 1C980 Ammonia crackers ......................................................................................................... 0B004.b.2.d Ammonia distillation towers .......................................................................................... 0B004.b.4.b Ammonia synthesis converters & units .................................................................................. 1B227 Ammonia-hydrogen exchange plant ................................................................................. 0B004.a.2 Ammonia-hydrogen exchange equipment and components ........................................... 0B004.b.2 Ammonium bifluoride ............................................................. see ammonium hydrogen fluoride Ammonium hydrogen fluoride ......................................................................................... 1C350.d.1 Ammonium nitrate, including certain fertilizers containing ammonium nitrate .................... 1C997 Export Administration Regulations Bureau of Industry and Security January 9, 2012Description ECCN Citation Ammunition hand-loading equipment .................................................................................... 0B986 Amorphous alloy strips ........................................................................................................... 1C003 Amplifiers, microwave solid state .................................................................................... 3A001.b.4 Amplifiers, pulse .................................................................................................................. 3A999.d Analog instrumentation tape recorders ............................................................................. 3A002.a.1 Analog computers ................................................................................................................... 4A101 Analog-to-digital converters ................................................................................................ 3A101.a Analog-to-digital converters, integrated circuits .............................................................. 3A001.a.5 Analog-to-digital conversion equipment ............................................................................. 4A003.e Analyzers, network .............................................................................................................. 3A002.e Analyzers, spectrum .......................................................................................................... 3A002.c.1 Andes virus ....................................................................................................................... 1C351.a.1 Angular displacement measuring instruments ..................................................................... 2B206.c Angular measuring instruments ........................................................................................ 2B006.b.2 Angular measuring instruments .............................................................................................. 2B206 Angular-linear inspection equipment (hemishells) ................................................................. 2B206 Angular-linear inspection equipment (hemishells) .............................................................. 2B006.c Angular rate sensors ................................................................................................................ 7A002 Animal pathogens .................................................................................................................. 1C352 Annealing or recrystallizing equipment ...................................................................... 3B991.b.1.c.1 Antennae, for microwave power source ............................................................................ 0B001.i.3 Antennae, phased array ...................................................................................................... 5A001.d Antennae, phased array (for radar) ...................................................................................... 6A008.e Anti-vibration mounts (noise reduction), civil vessels .................................................. 8A002.o.3.a Antimony hydrides.................................................................................................................. 3C004 Aramid fibers & filamentary materials ................................................................................ 1C210.a Aramid fibers & filamentary materials ................................................................................ 1C010.a Arc remelt & casting furnaces ............................................................................................. 2B227.a Argon ion lasers ................................................................................................................... 6A205.a Argon ion lasers ................................................................................................................... 6A005.a Armor body ............................................................................................................................. 1A005 Armor plate drilling machines ............................................................................................. 2B018.a Armor plate planing machines ............................................................................................. 2B018.b Armor plate quenching presses ............................................................................................ 2B018.c Arms machinery, equipment, gear, parts, and accessories ..................................................... 2B018 Arms (small) chambering machines .................................................................................... 2B018.o Arms (small) deep hole drilling machines and drills therefor ............................................. 2B018.p Arms (small) rifling machines ............................................................................................. 2B018.q Arms (small) spill boring machines ......................................................................................2B018.r Aromatic polyimides ......................................................................................................... 1C008.a.3 Aromatic polyamide-imides .............................................................................................. 1C008.a.2 Aromatic polyetherimides ................................................................................................. 1C008.a.4 Export Administration Regulations Bureau of Industry and Security January 9, 2012Description ECCN Citation Array processor microcircuits .................................................................................. 3A001.a.3 Note Array processors/assemblies ................................................................................................. 4A004 Array processors/assemblies ................................................................................................... 4A003 Arrays of aerosol generating units or spray booms, specially designed forfitting to aircraft, “lighter than air vehicles,”or “UAVs”............................................ 2B352.h.2 Arsenic trichloride ............................................................................................................ 1C350.b.1 Arsenic hydrides ..................................................................................................................... 3C004 Asphalt paving mixtures ......................................................................................................... 1C980 Aspheric optical elements .................................................................................................... 6A004.e Assemblies to enhance performance by aggregation of computing elements ..................... 4A994.c Asynchronous transfer mode (ATM) equipment ......................................................... 5A991.c.10.d Asynchronous transfer mode (ATM), technology for the developmentof equipment employing ................................................................................................ 5E001.c.1 Atomic vapor laser isotope separation plant ..................................................................... 0B001.a.6 Atomic vapor laser isotope separation process equipment .................................................. 0B001.g Atomic frequency standards ................................................................................................ 3A002.g Atomic transition solid state lasers ...............................................................................6A005.a or b Attitude Heading Reference Systems (AHRS), source code software ................................... 7D002 Attitude control equipment for missiles .................................................................................. 7A116 Aujeszky’ disease virus (Porcine herpes virus) ................................................................ 1C352.a.6 Austenitic stainless steel plate, valves, piping, tanks and vessels ....................................... 2B999.n Autoclave temperature, pressure or atmosphere regulation technology ................................. 1E103 Autoclaves, ovens and systems ............................................................................................ 0B002.a Automated control systems, submersible vehicles .............................................................. 8A002.b Automatic drug injection systems ........................................................................................... 0A981 Automotive, diesel, and marine engine lubricating oil ........................................................... 1C980 Avian influenza virus ........................................................................................................ 1C352.a.2 Aviation engine lubricating oil ............................................................................................... 1C980 Avionic equipment, parts, and components ............................................................................ 7A994 Avionics EMP/EMI protection technology ............................................................................ 7E102 Bacillus anthracis .............................................................................................................. 1C351.c.1 Bacteria ................................................................................................................................ 1C351.c Bacteria ................................................................................................................................ 1C354.a Bacteria ................................................................................................................................ 1C352.b Baffles .................................................................................................................................. 0A001.h Baffles (rotor tube), gas centrifuge ................................................................................... 0B001.c.7 Balancing machines ............................................................................................................. 2B119.a Balancing machines ............................................................................................................. 7A104.a Balancing instrumentation ................................................................................................... 7A104.b Balancing machines, centrifugal multiplane ........................................................................... 2B229 Balancing mahcines, centrifugal multiplane ....................................................................... 2B999.m Ball bearings, precision hardened steel and tungsten carbide .............................................. 1C999.a Export Administration Regulations Bureau of Industry and Security January 9, 2012Description ECCN Citation Ball bearings, Radial ............................................................................................................... 2A101 Ball & solid roller bearings .................................................................................................. 2A001.a Band-pass filters, tunable .................................................................................................. 3A001.b.5 Barium metal vapor lasers ................................................................................................... 6A005.b Bartonella quintana ........................................................................................................... 1C351.b.1 Batch mixers .......................................................................................................................... 1B117 Bathymetric survey systems .......................................................................................... 6A001.a.1.b Batons, shock .......................................................................................................................... 0A985 Batons, spiked ......................................................................................................................... 0A983 Bay cable systems .......................................................................................................... 6A001.a.2.e Bay cable systems software ............................................................................................ 6D003.a.3 Bayonets ............................................................................................................................... 0A918.b Beam lead bonders, stored program controlled equipment ........................................... 3B991.b.3.b Beam steering mirrors ....................................................................................................... 6A004.a.4 Beamforming techniques ............................................................................................... 6A001.a.2.c Bearings, ball & solid roller ................................................................................................. 2A001.a Bearings, gas centrifuge .................................................................................................... 0B001.c.4 Bearings, gas centrifuge .................................................................................................... 0B001.c.5 Bearings, high precision/temperature/special ......................................................................... 2A001 Bearings, magnetic (active) ................................................................................................. 2A001.c Bearings, magnetic (suspension) ...................................................................................... 0B001.c.4 Bearings, precision hardened steel and tungsten carbide ..................................................... 1C999.a Bellow valves .................................................................................................................... 0B001.b.1 Bellow valves .................................................................................................................. 0B001.d.6 Bellows forming dies ........................................................................................................... 2B999.b Bellows manufacturing equipment ...................................................................................... 2B999.b Bellows or rings, gas centrifuge........................................................................................ 0B001.c.6 Bellows seal valves ............................................................................................................... 2A226 Bellows seal valves .............................................................................................................. 2B350.g Bellows sealed valves, n.e.s. ................................................................................................. 2A999 Bellows-forming mandrels................................................................................................... 2B228.c Bellows-forming dies ........................................................................................................... 2B228.c Benzilic acid...................................................................................................................... 1C350.b.2 Beryllium metal, alloys, compounds, or manufactures......................................................... 1C230 Beryllium metal particulate......................................................................................... 1C111.a.2.a.4 Beryllium/beryllium substrate blanks .................................................................................. 6C004.d Biological containment facilities, ACDP level 3 or 4 .......................................................... 2B352.a Biological isolators ............................................................................................................2B352.f.2 Biological manufacturing equipment & facilities ................................................................... 2B352 Biological safety cabinets ..................................................................................................2B352.f.2 Bismaleimides ................................................................................................................... 1C008.a.1 Bismuth ................................................................................................................................... 1C229 Export Administration Regulations Bureau of Industry and Security January 9, 2012。
匹配场处理检测因子
匹配场处理检测因子下载温馨提示:该文档是我店铺精心编制而成,希望大家下载以后,能够帮助大家解决实际的问题。
文档下载后可定制随意修改,请根据实际需要进行相应的调整和使用,谢谢!并且,本店铺为大家提供各种各样类型的实用资料,如教育随笔、日记赏析、句子摘抄、古诗大全、经典美文、话题作文、工作总结、词语解析、文案摘录、其他资料等等,如想了解不同资料格式和写法,敬请关注!Download tips: This document is carefully compiled by the editor. I hope that after you download them, they can help yousolve practical problems. The document can be customized and modified after downloading, please adjust and use it according to actual needs, thank you!In addition, our shop provides you with various types of practical materials, such as educational essays, diary appreciation, sentence excerpts, ancient poems, classic articles, topic composition, work summary, word parsing, copy excerpts,other materials and so on, want to know different data formats and writing methods, please pay attention!匹配场处理检测因子在现代科学研究中扮演着重要角色,通过匹配场处理检测因子的测定,可以更准确地分析样品中的成分和含量。
课堂教学反思模板全英
---Title: Classroom Teaching Reflection on [Subject]Date: [Date of Reflection]Teacher's Name: [Your Name]Subject: [Subject Taught]Grade Level: [Grade Level]---Introduction:The purpose of this reflection is to critically analyze and evaluate my recent classroom teaching experience. By examining my teaching methods, student engagement, and the overall learning outcomes, I aim to identify areas of strength and areas for improvement. This reflection will help me refine my teaching strategies and enhance the learning experience for my students.---Teaching Methods and Techniques:1. Lesson Planning:- What were the main objectives of the lesson?- How did the lesson plan align with the curriculum standards?- Did the lesson plan allow for flexibility to cater to diverse learning needs?2. Instructional Strategies:- What teaching methods did I use (e.g., lectures, discussions, group work)?- Were the methods appropriate for the subject and the students' learning styles?- Did the strategies promote active learning and critical thinking?3. Assessment:- How did I assess student understanding and progress?- Were the assessment methods effective in measuring learning outcomes?- Did the assessments provide constructive feedback for students?---Student Engagement and Participation:1. Student Interaction:- How did students respond to the lesson activities?- Were there any students who were particularly engaged or disengaged?- How did I encourage participation from all students?2. Group Dynamics:- How did group work contribute to the learning process?- Were there any challenges in managing group dynamics?- How did I address conflicts or issues within groups?3. Individual Student Feedback:- Did I provide timely and specific feedback to students?- How did students react to the feedback?- Did the feedback help students to improve their understanding or performance?---Learning Outcomes:1. Achievement of Objectives:- Did the majority of students achieve the learning objectives?- Were there any gaps in understanding that need to be addressed?- How did the lesson contribute to the overall curriculum?2. Student Progress:- How did individual students progress throughout the lesson?- Were there any students who needed additional support or challenge?- How did I adapt my teaching to cater to different learning levels?3. Overall Satisfaction:- How satisfied were the students with the lesson?- Did the lesson meet their expectations?- How could the lesson be improved to enhance student satisfaction?---Areas for Improvement:1. Teaching Methods:- What specific teaching strategies could be more effective?- How can I incorporate more interactive or technology-based learning activities?- What additional resources or materials could enhance the learning experience?2. Student Engagement:- How can I better cater to diverse learning styles and needs?- What strategies can I use to increase student participation and engagement?- How can I foster a more inclusive and supportive classroom environment?3. Assessment and Feedback:- What types of assessments would be more beneficial for this lesson?- How can I provide more meaningful and actionable feedback?- How can I use formative assessments to inform my teaching and adapt to student needs?---Conclusion:This reflection has provided me with valuable insights into my recent classroom teaching experience. By acknowledging both my strengths and areas for improvement, I am better equipped to refine my teaching practices and create a more effective and engaging learning environment for my students. I look forward to applying these learnings to future lessons and continuing to grow as an educator.---References:- [Any relevant educational theories or resources used in the reflection]---[Your。
需求侧响应下主动配电网优化调度
第41卷 第2期吉林大学学报(信息科学版)Vol.41 No.22023年3月Journal of Jilin University (Information Science Edition)Mar.2023文章编号:1671⁃5896(2023)02⁃0207⁃10需求侧响应下主动配电网优化调度收稿日期:2022⁃06⁃10基金项目:黑龙江省自然科学基金资助项目(LH2019E016)作者简介:高金兰(1978 ),女,山西运城人,东北石油大学副教授,主要从事电力系统运行与稳定㊁新能源发电研究,(Tel)86⁃136****6089(E⁃mail)jinlangao@㊂高金兰,孙永明,薛晓东,刁 楠,侯学才(东北石油大学电气信息工程学院,黑龙江大庆163318)摘要:针对电网运行中能量调度不佳的问题,首先基于需求侧响应不确定性特点,引入非经济因素以及消费心理学特征,建立需求侧响应模型;其次使用拉丁超立方抽样(LHS:Latin Hypercube Sampling)改善初始种群质量,引入正弦因子提高局部搜索能力,并实行变异操作优化全局搜索精度,以解决麻雀算法(SSA:Sparrow Search Algorithm)的早熟等问题;最后需求侧响应以电网运行成本和环境成本最小为目标建立主动配电网优化调度模型,并使用改进的麻雀算法进行求解㊂仿真结果验证了提出模型的准确性,算法的高效性,有效解决了能量调度不佳的问题㊂关键词:需求侧响应;改进麻雀算法;主动配电网;非经济因素中图分类号:TP302;TM734文献标志码:AOptimal Dispatch of Active Distribution Network under Demand Side ResponseGAO Jinlan,SUN Yongming,XUE Xiaodong,DIAO Nan,HOU Xuecai(School of Electrical and Information Engineering,Northeast Petroleum University,Daqing 163318,China)Abstract :Demand side response is an important means of active distribution network optimization scheduling.Aiming at the problem of poor energy scheduling in power grid operation,firstly,based on the uncertainty characteristics of demand side response,introducing non⁃economic factors and characteristics of consumer psychology,the active distribution network optimization is modeled with the minimum power grid operation cost and environmental cost as the objective function;secondly,aiming at the premature problem of sparrow algorithm,latin hypercube sampling is used to improve the initial population quality,sine factor is introduced to improve the local search ability of the algorithm,and mutation operation is implemented to optimize the global search accuracy of the algorithm;finally,the improved sparrow search algorithm is applied to the solution of the active power grid optimization model.The simulation results verify the accuracy of the proposed model and the efficiency of the algorithm,and effectively solve the problem of poor energy scheduling.Key words :demand side response;improved sparrow search algorithm;active distribution network;non⁃economic factors 0 引 言随着电力改革的深入发展,新的电力需求也随之而来㊂对分布式电源广泛接入电网带来的能量调度问题,主动配电网的提出对改善该问题是一个行之有效的手段[1]㊂需求侧响应技术是主动配电网的一种典型调度方式,可通过不同的定价措施以及政策导向引导用户改变用电习惯[2],可协调用户的负荷改善能力,调节整体的峰谷用电曲线,平衡各阶段用电器数量,其经济成本低㊁适用范围广㊂在主动配电网发展迅猛的今天,对需求侧响应技术的研究在改善用电质量㊁提升用户用电体验以及合理调配区域内有限电力资源方面有着重要意义㊂目前,对需求响应有许多学者进行相关研究㊂张智晟等[3]通过对不同时刻的电价信息响应程度进行负荷转移率的求解,将用户消费习惯与需求响应进行有效结合,通过实验证明了需求响应中考虑多种因素的重要性㊂许汉平等[4]主要应用政策激励进行需求响应,以整体能源的利用率㊁经济成本为优化目标,建立多方面调度模型㊂张超等[5]依据电力市场定义下,用电量以及电力价格的线性关系进行需求响应技术实施㊂在忽略储能成本的前提下,进行分布式能源㊁储能㊁电网等大规模功率交互条件下的综合优化㊂艾欣等[6]在直接负荷控制下进行整体的耦合系统优化模型建立,通过实验结果验证了需求响应能进行高低时段负荷调节,可有效缓解高峰时段用电压力,使负荷供需趋于平衡㊂朱超婷等[7]通过对电价弹性矩阵的建立进行负荷需求模拟,考虑用电量交互㊁需求响应成本等建立电网成本最低优化目标㊂上述研究并未考虑价格型响应在经济因素以外的影响,以及多种响应协调优化的情况㊂笔者在上述研究的基础上,引入非经济因素影响的电价型响应,以及攀比心理㊁从众心理影响的激励型响应,建立以经济㊁环境成本最小为目标的主动配电网优化模型㊂为精确求解模型,提出一种改进的麻雀算法,在基本算法中加入拉丁超立方抽样㊁正弦因子和变异操作㊂通过IEEE33节点算例,验证了笔者提出的模型和算法的准确性㊂1 需求侧响应1.1 价格型响应在消费心理学的描述中,价格的高低会影响消费者的选择㊂对电价而言,电价的差值大小和浮动范围都会影响需求响应的波动㊂用户的主观意愿在价格的影响下会频繁的改变,具有强烈的不确定性,其行为用曲线表示会有相应的上下限,定义为乐观曲线与悲观曲线[3],以不同时段的价格变化为基础,对应相应的负荷变化率,利用Logistic函数对负荷转移率进行描述如下:λpv(Δp pv)=a1+e-(Δp pv-c)/μ+b,(1)其中a为限制变化范围值;b为可变化参数;c为电价近似中间值;μ为调节参数;λpv为电价响应负荷转移率,Δp pv为电价差值㊂对不同响应区用户行为特征的负荷转移如下:λzpv=λmax pv+λmin pv2,0≤Δp pv≤a pv,λmin pv+λmaxpv+λmin pv2(1+m),a pv≤Δp pv≤b pv,λmax pv,Δp pv≥b pvìîíïïïïïï,(2)m=Δp pv-a pvb pv-a pv,(3)其中a pv㊁b pv分别为不同电价差分段点;λzpv为负荷峰谷转移率;λmax pv为最大峰谷转移率;λmin pv为最小峰谷转移率㊂同理,分别求出峰转平㊁平转谷的实际负荷转移率λzpf㊁λzfv㊂在需求侧响应过程中,用户并不只会从价格差值方面改变负荷大小㊂上述模型只能表示用户受经济因素影响进行相应决策,而实际电网运行过程中用户所面临的影响远远不止经济因素一种㊂在实际过程中,用户在价格差异的刺激下想要进行负荷转移,但存在由于条件限制没办法完成此操作的情况,如后续时间段有其他任务无法在当前时间段转移负荷,即各种非经济因素导致的约束㊂为符合实际负荷转移情况,笔者提出非经济因素影响的负荷转移曲线,并引入心理学特征,实际负荷转移曲线类似于倒S型曲线,其负荷转移概率(λfz)与非经济因素(f)关系如图1所示㊂图1可用公式表示为λfz=h(1+e1-l/f)-1,(4)其中h为基础系数;l为条件系数㊂802吉林大学学报(信息科学版)第41卷图1 负荷转移概率曲线Fig.1 Load transfer probability curve 综合考虑经济因素以及非经济因素对负荷转移概率的影响,可得用户响应的转移量Q t =-λzpf L p λfz -λzpv L p λfz ,t ∈T p ,λzpf L p λfz -λzfv L f λfz ,t ∈T f ,λzpv L p λfz +λzfv L f λfz,t ∈T v ìîíïïïï㊂(5)以及转移后负荷总量L t =L 0+Q t ,(6)其中λzpf 为峰转平时段转移率;λzfv 为平转谷时段转移率;L p ㊁L f 分别为峰㊁平时段原始平均负荷;T p ㊁T f ㊁T v 分别为峰㊁平㊁谷3时段,L 0为电价响应前负荷㊂1.2 激励型需求响应直接负荷控制(DLC:Direct Load Control)㊁可中断负荷(IL:Interruptible Load)激励响应适应条件简洁,应用较为广泛㊂二者均是与电力公司或电网管理部门提前签署的负荷控制协议㊂前者相对后者协议的自由度更高,并且没有IL 在不按照协议规定动作时的违约惩罚政策㊂1.2.1 直接负荷控制为在储能设备应用频繁的情况下充分发挥其双向交互的优势[8],签订DLC 协议的用户在满足基本的协议容量要求下,可在一定限度内通过储能设备人为增减响应程度㊂传统的激励型响应并未考虑人本身的不确定因素,为此笔者引入心理学中攀比心理以及从众心理因素,即在同一区域内用户签订相应供电协议后,会根据其他参与协议人数的变化在约定改变负荷期间进行相应变化㊂结合响应人群的心理特点,构建响应模型如下:D DLC =∑24t =1D DLC t +∑24t =1(E +t +E -t )α,(7)其中D DLC t 为DLC 协议响应量;D DLC 为响应后负荷;E +t ,E -t 为不同时间段增减负荷大小;α为响应系数㊂1.2.2 中断负荷在IL 规划中考虑违约协议部分,并依据上述心理学因素,在DLC 响应量变化时IL 也会随之变化,二者协同作用,建立中断负荷情况下的负荷响应模型如下:Q IL =∑24t =1(P IL,t -P wx,t ),P IL,t =rP wx,t {,(8)其中P IL,t 为IL 协议响应量;P wx,t 为中断响应未响应负荷;r 为违约响应系数㊂2 考虑需求侧响应的主动配电网优化模型2.1 目标函数目标函数包括经济与环境成本两部分,经济成本主要为储能维护㊁新能源发电㊁需求侧响应补偿和网络损耗成本,表达式为F 1=min ∑24t =1P x ,t C pvq +∑24t =1P bat,t C cn +∑24t =1P grid,t C g,t +B MG +B DLC +B IL +B []loss ,(9)其中P x ,t ㊁P bat,t ㊁P grid,t 分别为新能源出力㊁储能出力㊁向上级电网购电量;C pvq ㊁C cn ㊁C g,t 为相应成本系数;B DLC 为DLC 成本;B IL 为IL 成本;B loss 为网损成本;B MG 为燃气轮机运行成本㊂新能源设备出力情况:P x ,t =P pv,t +P wind,t ,(10)其中P pv,t ㊁P wind,t 分别为光伏㊁风机发电功率㊂燃气轮机运行成本:902第2期高金兰,等:需求侧响应下主动配电网优化调度B MG =∑24t =1P MG,t ηMG L p gas ,(11)其中ηMG 为效率;L 为热值;p gas 为气价;P MG,t 是燃气轮机功率㊂需求侧响应成本:B DLC =∑24t =1C DLCD DLC t +∑24t =1(E +t d +t +E -t d -t )α,(12)B IL =∑24t =1(C IL P IL,t -C wx P wx,t ),(13)其中C DLC 为DLC 补偿价格;d +t ㊁d -t 为增减负荷价格;C IL ㊁C wx 为IL 补偿价格㊁惩罚价格㊂网损成本:B loss =∑24t =1C g,t ∑Nj =1u j ,t ∑k ∈Ωj u k ,t G jk cos δjk ,t ,(14)其中N 为节点总数;u j ,t ㊁u k ,t 为t 时刻节点j ㊁k 电压幅值;G jk 为节点j ㊁k 间电导;Ωj 为以节点j 为首节点的尾节点集合;δjk ,t 为t 时刻节点j ㊁k 间电压相角差㊂环境成本即污染物处理成本最低,表达式为F 2=min ∑24t =1P grid,t W g C 1+∑24t =1P MG,t W MG C []2,(15)其中W g ㊁W MG 分别为向上级购买电量产生的污染物系数㊁燃气轮机污染系数;C 1㊁C 2为成本系数㊂2.2 动态权重调整主动配电网优化目标包括经济和环境成本两方面,可采用引入动态权重因子对综合成本进行实时优化[9]㊂对整个周期相同时间范围内的成本函数进行归一化处理,即可得到F 1(t )㊁F 2(t ),通过动态权重因子进行实时优化得到总目标函数:min f =∑24t =1[xF 1(t )+yF 2(t )],x =c 1+c 2F 1(t ),y =1-x ìîíïïïï,(16)其中x 为经济权重系数;y 为环境权重系数;c 1㊁c 2为变化因子㊂2.3 约束条件功率平衡约束为P MG +P pv +P wind +P bat +P grid =P load +P loss +P DR ,(17)其中P MG ㊁P pv ㊁P wind ㊁P bat ㊁P grid ㊁P load ㊁P loss ㊁P DR 分别为燃气轮机㊁光伏㊁风机㊁储能㊁上级电网传输㊁初始负荷㊁网损和需求响应功率㊂储能运行约束为E bat,t =E bat,t -1+(P c,t ηc -P d,t ηd )Δt ,(18)E min bat ≤E bat ≤E max bat ,(19)其中E max bat ㊁E min bat 分别为储能元件最大最小储量;E bat,t 为当前时刻储能元件储量;E bat,t -1为储能元件上一时刻余量;ηc ,ηd 分别为充放电效率;P c,t ㊁P d,t 分别为充放电功率㊂燃气轮机约束为P min ≤P MG ≤P max ,(20)其中P min ,P max 分别为燃气轮机出力上下限㊂除上述约束外,其他诸如节点电压约束等如文献[7]所描述㊂3 模型求解3.1 原始麻雀算法麻雀算法(SSA:Sparrow Search Algorithm)是对麻雀种群觅食过程中发生的一系列行为的分步012吉林大学学报(信息科学版)第41卷分析[10],具体原理如下㊂发现者位置更新:X t+1i,d=X t i,d exp-iαT()max,R2<S,X t i,d+Q L,R2≥Sìîíïïï,(21)其中X t i,d为第i只麻雀d维位置;T max为迭代次数上限值;α∈(0,1]为随机数;R2㊁S分别为危险值和正常值;Q为随机数;L为1×D的矩阵㊂跟随者位置更新:X t+1i,d=Q exp X t W i,d-X t i,diæèçöø÷2,i>n2,X t bi,d+X tb i,d-X t i,d A+L,其他ìîíïïïï,(22)其中X t Wi,d 为最差位置;X t bi,d为最好位置;A+=A T(A T A)-1,A为全为1或-1的矩阵㊂预警者位置更新:X t+1i,d=X t i,d+βX ti,d-X b t i,d,X t i,d+K X t i,d-X W t i,d(f i-f w)+æèçöø÷ε,ìîíïïïï(23)其中β为(0,1)的正态分布随机数;K为[-1,1]的随机数;f i为当前个体适应度;f g为最优个体适应度;f w为最差个体适应度㊂3.2 改进算法3.2.1 改善初始种群对智能算法,初始种群较差会对算法寻优过程产生一定负面影响,为避免由于初始种群造成局部最优现象,采用拉丁超立方抽样产生初始种群,具体步骤如下:1)确定一个初始种群规模T;2)将每一维量的可行区域分割成T个长度均一的区域,即H n个超立方体;3)建立矩阵B(H×n),其每行即为一个被抽到的超立方体;4)在不同抽中的超立方体中随机得到样本,即为初始种群的值㊂3.2.2 引入正弦权重系数为避免麻雀算法早熟现象,先引入粒子群算法的粒子移动概念,将跳跃到最优解的方式变为正常移动,并去除向原点收敛操作㊂再引入正弦变化的权重系数,具体如下㊂发现者:X t+1i,d=X t i,d(1+Q),R2<S,ωX t i,d+Q,R2≥S{㊂(24) 跟随者:X t+1i,d=ωX tb i,d+1D∑D d=1(K(X t b i,d-X t i,d))㊂(25) 权重系数:ω=ωmin+ωmax+ωmin2sinπt t()max,(26)其中ωmax为权重峰值;ωmin为权重谷值;t为当前迭代次数;t max为迭代次数峰值㊂对预警者改变跟随方式:X t+1i,d=X t i,d+β(X t i,d-X t bi,d),f i≠f g,X t i,d+β(X t Wi,d-X t bi,d),f i=f g{㊂(27)112第2期高金兰,等:需求侧响应下主动配电网优化调度3.2.3 变异操作变异操作能在一定程度上改善个体均一性,提升整体寻优效果[11⁃12]㊂在算法流程中引入变异概念对当前适应度最差的10%个体进行替换,并且按照自然进化的方式对变异概率进行合理变化,以平衡寻优进程,变异过程和概率为X new i ,d =X now i ,d +p m X now i ,d ,(28)p m =p max -∑N i =1(f i -f avg )2N p ,(29)其中X new i ,d 为变异后个体;X now i ,d 为变异前个体;P max 为变异频率上限;f i ㊁f avg 分别为个体的适应度㊁种群中所有个体的平均适应度;p 为变异频率调节参数㊂3.3 基于改进SSA 的主动配电网优化调度求解步骤依据主动配电网优化调度模型选取合适控制变量,麻雀个体位置的优劣代表目标函数的优化程度㊂通过麻雀群体避让天敌的行为进行位置更新,迭代到最优位置,即最佳优化调度结果,其流程图如图2所示,具体步骤如下:Step 1 输入主动配电网参数,包括新能源㊁储能设备等出力大小和负荷大小,以及分时电价㊁补偿价格等;Step 2 设置改进麻雀算法的初始数据,即迭代次数㊁权重系数㊁种群大小和变异概率等;Step 3 采用LHS 初始麻雀种群;Step 4 进行改进麻雀算法操作,根据粒子移动概念进行发现者㊁跟随者位置更新;在全维度进行警戒者位置更新;Step 5 判断是否进行终止操作,是则输出最优结果;Step 6 未达到截至条件,进行变异操作,将部分劣等个体进行变异,替代变异前个体,重新返回Step4进行循环,直至达到截至条件㊂图2 主动配电网优化调度流程图Fig.2 Optimal dispatching flow chart of active distribution network 4 算例分析4.1 仿真参数笔者采用修改后的IEEE33节点系统(见图3)验证整体模型的效果㊂节点17㊁18㊁24㊁25接入价格响应负荷;节点30㊁31㊁32接入激励响应用户;光伏接入节点15;风机接入节点4;燃气轮机接入节点21;储能设备接入节点23㊂DLC 补偿成本为0.3元/(kW㊃h),IL 的补偿成本为0.5元/(kW㊃h)㊂24h 的风光出力㊁负荷情况如图4所示,需求侧模型参数设置㊁区域内电价划分方式参照文献[13]㊂储能设备允许的SOC(State Of Charg)波动为0.2~0.9;燃气轮机的效率为0.85;光伏风机的维护成本为0.3元/(kW㊃h)㊂212吉林大学学报(信息科学版)第41卷图3 改进IEEE33节点图Fig.3 Improved IEE33node diagram 图4 主动配电网新能源出力、负荷曲线Fig.4 New energy output and load curve of active distribution network 4.2 仿真分析设置4种场景㊂场景1:电网不执行需求响应及优化㊂场景2:电网执行价格型需求响应㊂场景3:电网执行激励型需求响应㊂场景4:电网执行多种需求响应㊂场景1㊁4的总体调度情况如图5所示㊂图5 不同场景主动配电网优化调度图Fig.5 Optimal dispatching diagram of active distribution network in different scenarios 场景1中,在夜间时段以及用电器数量增加时,储能装置进行放电调节,在用电器数量减少以及新能源出力充足时进行充电调节,充分发挥其高发低储作用㊂燃气轮机在新能源出力不足及负荷升高时进行出力,减少相应的购电功率㊂在场景4中,需求侧响应技术的加入,在负荷高峰8⁃14h㊁20⁃23h 负荷相应减少,且部分负荷转移到1⁃6h㊂由于考虑环境成本以及动态优化条件,所以燃气轮机出力减少㊂对比场景1,场景4仅在20h㊁21h 燃气轮机工作㊂由图5可知,笔者提出的模型可有效调节不同阶段设备出力情况,合理实现一个周期内的总体调度㊂大电网㊁新能源发电以及储能设备协同作用,对区域内进行整体负荷供电㊂不同情况下需求侧响应前后负荷对比如图6㊁图7所示㊂可以看出3种情况均有削峰填谷效果,单一的需求响应在削峰填谷综合方面都有一定局限性㊂312第2期高金兰,等:需求侧响应下主动配电网优化调度图6 单一需求侧响应负荷变化曲线Fig.6 Response load curve of single demandside 图7 多种需求侧响应负荷变化曲线Fig.7 Response load change curves of multiple demand side 价格型响应下,7⁃11h 负荷减少约5%,12⁃14h几乎无变化,夜晚峰时段负荷减少约3%,谷时段1⁃7h 负荷提升3.3%㊂激励型响应下,夜晚峰时段负荷减少约5%,7⁃11h 几乎无变化,谷时段1⁃7h 负荷无升高㊂而综合两种响应模式所得结果在峰谷时段优于单一模式,峰时段均有5%以上负荷削减量,低谷时段负荷也有序上升㊂不同情况下的综合成本值如表1所示,与不进行需求侧响应相比,单一型需求响应以及多种需求响应结合可以通过响应措施进行负荷改变,使成本降低10%~20%㊂相比于场景1,场景4成本减少1242元,可有效降低整体的综合成本㊂表1 不同场景下成本情况 Tab.1 Cost under different scenarios 元场景1234经济成本4050.53791.83797.73109.6环境成本1756.31532.31425.11355.2总成本5706.85324.15222.84464.8 在调度周期内经济㊁环境权重变化情况如图8所示㊂在1⁃9h 经济权重递增趋势较大,从0.33递增到0.359,减少相应经济成本;17⁃21h 环境权重上升,对污染排放加以限制㊂对动态权重在一个调度周期内进行不间断调节,以减少整体成本㊂图8 动态权重变化图Fig.8 Dynamic weight change diagram 笔者分别采用灰狼优化算法(GWO:Grey Wolf Optimizer)㊁原始麻雀算法㊁鲸鱼优化算法412吉林大学学报(信息科学版)第41卷 图9 算法对比图 Fig.9 Algorithm comparison (WOA:Whale Optimization Algorithm)以及笔者的改进麻雀算法进行主动配电网优化,对比结果如图9所示㊂从图9中可看出,改进SSA 在整体迭代过程中稍优于其他算法㊂LHS㊁引入正弦权重㊁变异操作让算法中麻雀个体具备初始优势,在前期可达到较高的收敛速度;变异㊁正弦权重的引入可让其具备更好的全局寻优能力㊂对比发现,GWO 与WOA 前期收敛能力不强,原始SSA 的寻优速度与改进SSA 较为接近,但改进SSA 寻优精度更高㊂5 结 论笔者在考虑多种因素影响需求响应的基础上,构建主动配电网优化模型,采用改进麻雀算法进行求解,通过IEEE33算例进行仿真验证,证明了笔者模型㊁算法的准确性,结论如下:1)笔者提出的模型可有效实现主动配电网的优化调度,当需求响应加入运行时,可与其他设备进行协同优化,增加削峰填谷效果,配合动态权重因子的实时优化,可降低电网的整体成本;2)采用LHS㊁正弦因子㊁变异策略改进麻雀算法,可改善种群丰富程度,提高算法的收敛效果,与WOA㊁GWO㊁SSA 算法相比,改进的麻雀算法可以更好地进行主动配电网优化调度,有效降低综合成本㊂参考文献:[1]吕智林,廖庞思,杨啸.计及需求侧响应的光伏微网群与主动配电网双层优化[J].电力系统及其自动化学报,2021,33(8):70⁃78.LÜZ L,LIAO P S,YANG X.Bi⁃Level Optimization of Photovoltaic Microgrid Group and Active Distribution Network Considering Demand Side Response [J].Journal of Power System and Automation,2021,33(8):70⁃78.[2]刘伟,王俊,龚成生,等.基于激励机制的家庭能量系统优化策略研究[J].吉林大学学报(信息科学版),2021,39(5):525⁃530.LIU W,WANG J,GONG C S,et al.Research on Optimization Strategy of Family Energy System Based on IncentiveMechanism [J].Journal of Jilin University (Information Science Edition),2021,39(5):525⁃530.[3]张智晟,于道林.考虑需求响应综合影响因素的RBF⁃NN 短期负荷预测模型[J].中国电机工程学报,2018,38(6):1631⁃1638,1899.ZHANG Z S,YU D L.RBF⁃NN Short⁃Term Load Forecasting Model Considering Comprehensive Influencing Factors of Demand Response [J].Chinese Journal of Electrical Engineering,2018,38(6):1631⁃1638,1899.[4]许汉平,李姚旺,苗世洪,等.考虑可再生能源消纳效益的电力系统 源⁃荷⁃储”协调互动优化调度策略[J].电力系统保护与控制,2017,45(17):18⁃25.XU H P,LI Y W,MIAO S H,et al.Power System Source Load Storage”Coordinated Interactive Optimal Dispatching Strategy Considering Renewable Energy Consumption Benefits [J ].Power System Protection and Control,2017,45(17):18⁃25.[5]张超,左高,腾振山,等.基于需求侧响应的配电网优化调度研究[J].智慧电力,2020,48(2):53⁃57,91.ZHANG C,ZUO G,TENG Z S,et al.Research on Optimal Dispatching of Distribution Network Based on Demand SideResponse [J].Smart Power,2020,48(2):53⁃57,91.[6]艾欣,陈政琦,孙英云,等.基于需求响应的电⁃热⁃气耦合系统综合直接负荷控制协调优化研究[J].电网技术,2019,43(4):1160⁃1171.AI X,CHEN Z Q,SUN Y Y,et al.Research on Coordinated Optimization of Integrated Direct Load Control of Electric Thermal Pneumatic Coupling System Based on Demand Response [J].Power Grid Technology,2019,43(4):1160⁃1171.[7]朱超婷,杨玲君,崔一铂,等.考虑需求响应用户参与度的主动配电网优化调度[J /OL].电测与仪表:1⁃9[2022⁃06⁃08].https:∥ /kcms /detail /23.1202.TH.20201217.1641.003.html.512第2期高金兰,等:需求侧响应下主动配电网优化调度612吉林大学学报(信息科学版)第41卷ZHU C T,YANG L J,CUI Y B,et al.Optimal Dispatching of Active Distribution Network Considering Demand Response and User Participation[J/OL].Electric Measurement and Instrument:1⁃9[2022⁃06⁃08].https:∥/kcms/detail/ 23.1202.TH.20201217.1641.003.html.[8]范宏,邓剑.不确定性的激励型需求响应对配电网可靠性的影响[J].现代电力,2020,37(4):416⁃424. FAN H,DENG J.Influence of Uncertain Incentive Demand Response on Distribution Network Reliability[J].Modern Power, 2020,37(4):416⁃424.[9]杨雪.计及柔性负荷的多时间尺度主动配电网优化调度研究[D].北京:北京交通大学电气工程学院,2018. YANG X.Research on Optimal Dispatch of Multi⁃Time Scale Active Distribution Network Considering Flexible Load[D]. Beijing:School of Electrical Engineering,Beijing Jiaotong University,2018.[10]薛建凯.一种新型的群智能优化技术的研究与应用[D].上海:东华大学信息科学与技术学院,2020.XUE J K.Research and Application of a New Swarm Intelligence Optimization Technology[D].Shanghai:College of Information Science and Technology,Donghua University,2020.[11]黄治翰,汪晗,李启迪,等.基于改进遗传算法的主动配电网经济优化调度[J].山东电力技术,2021,48(10): 12⁃16,65.HUANG Z H,WANG H,LI Q D,et al.Economic Optimal Dispatch of Active Distribution Network Based on Improved Genetic Algorithm[J].Shandong Electric Power Technology,2021,48(10):12⁃16,65.[12]王彦琦,张强,朱刘涛,等.基于改进鲸鱼优化算法的GBDT回归预测模型[J].吉林大学学报(理学版),2022,60 (2):401⁃408.WANG Y Q,ZHANG Q,ZHU L T,et al.GBDT Regression Prediction Model Based on Improved Whale Optimization Algorithm[J].Journal of Jilin University(Science Edition),2022,60(2):401⁃408.[13]徐青山,曾艾东,王凯,等.基于Hessian内点法的微型能源网日前冷热电联供经济优化调度[J].电网技术,2016, 40(6):1657⁃1665.XU Q S,ZENG A D,WANG K,et al.Hessian Interior Point Method Based Economic Optimal Dispatch of Day Ahead Combined Cooling,Heating and Power Generation in Micro Energy Network[J].Power Grid Technology,2016,40(6): 1657⁃1665.(责任编辑:刘俏亮)。
Optimal feed policy for fed-batch fermentation of ethanol
Greek symbols specific growth rate of biomass (h–1) maximum specific growth rate at zero ethanol m0 concentration (0.4 h–1)
1 Introduction The reducing of air pollution has increased interest in the development of ethanol production. Fermentation technology has been dominated by the need of ethanol industry. The earliest fermentation systems were operated as batch processes. Fed-batch system has been shown to be feasible improving productivity. Such operation has been found to be particularly effective for processes in which effects such as substrate inhibition, catabolite repression, production inhibition and auxotrophic mutation are important [1, 2]. These phenomena lead to unimodal reaction rate expressions which exhibit a maximum with respect to a single reactant concentration or in terms of two or more reactant concentrations. In a fed-batch fermentation, the substrate concentration can be maintained at a fairly low level and unfavorable effects of a high concentration, such as growth inhibition, can be avoided. Because of this advantage, fed-batch fermentation techniques have been developed for many bioprocesses [3–8]. In such cases, depending on the cultivation process employed, the optiReceived: 23 September 1996 mum feed strategy of the substrate should be investigated F.S. Wang, C.H. Shyu so as to obtain maximum production. Department of Chemical Engineering, A number of publications have appeared investigating National Chung Cheng University, the effects of temperature and pH on the kinetics of ethanol Chia-Yi 621, Taiwan production by Zymomonas mobilis in batch or fed-batch fermentation [9–11]. The interest in Z. mobilis arises due to Correspondence to: F.S. Wang the well-known fact that this organism can convert glucose Financial support from the National Science Council of the R.O.C. efficiently and rapidly to ethanol with higher yields and under grant NSC85-2214-E194-004 is gratefully acknowledged. higher ethanol productivities than industrially useful
环境工程翻译译文
可回收的胺化超交联聚合物有效去除焦化废水的有机物关键词:废水有机物生物处理焦化废水高分子吸附剂出水有机物分馏荧光光谱学摘要出水有机物(EFOM)是一种复杂的有机物质主要来自生物处理污水,被认为是约束进一步深度处理主要因素。
在这里,可回收的胺化的超高交联吸附树脂(nda-802)具有胺基官能团,比表面积大,和足够的合成微孔区有效去除焦化废水生化出水(btcw)有机物,影响了其去除特性。
发现疏水部分是EfOM的主要成分,而且还发现疏水性 - 中性级分具有最高的SUVA水平(7.06毫克每毫升),这一点明显不同于国内废水. 柱吸附实验表明,对于EFOM nda-802来说它比其他聚合物吸附剂例如 d-301,XAD-4树脂,具有更高的吸附效率,而且效率可以按连续28批实验周期那样很稳定地持续下去。
此外,溶解有机物(DOM)分离和三维荧光光谱(EEM)的研究表明,nda-802表现出有吸引力的选择性吸附特性以及具有疏水性和芳香族化合物的去除效率高。
这可能归因于功能性胺基基团的存在,相对大的比表面积和独特的聚合物微孔的区域,nda-802对EFOM的去除具有效率高和可持续,并提供了一个潜在的替代的先进的处理方法。
1 概述随着城市化和工业化的进程,出水有机物(EFOM)从生物处理后的污水(BTSE)已经成为一个受纳水体有机污染物的主要来源。
EFOM在本质上是高度异质性(Quaranta等人。
,2012),天然有机物(NOM)主要是由来自地表水,可溶性微生物产物(SMP)的生物处理,有机化合物(SOC)的生产和使用有机化合物(Shon等人。
,2006b)。
一般来说,废水中COD大多数是由于EfOM,因此,有效去除EfOM成为主要的任务,但提高回收废水的质量或满足越来越严格的标准是有挑战性的任务。
大多数EfOM存在可溶性成分,而且以及构成了80%的COD (Shon等人。
,2006b),其有效去除仍然是一个具有挑战性的任务。
《高铁票价定价模型分析国内外文献综述3700字》
高铁票价定价模型研究国内外文献综述1国内研究现状国内有些学者聚焦对不同交通方式之间票价的影响因素开展研究。
刘莉文&张明[13]在梳理高速铁路和高速公路在各自因素条件下的经济运输距离,在此基础上制定不同经济运输距离条件下的运输资源优化策略;陶莉[14]比较分析交通运输行业不同运输方式的优劣势,并以京沪高速铁路为案例对象,结合高铁价格比较模型,得出了短途、中长途、长途等不同铁路运输方式之间的价格比较关系及相应的优势领域,指出高铁票价直接影响高速铁路作用的发挥和使命的实现。
王欢[15]在进行问卷调查的基础上,详细研究了不同收入群体在铁路交通运输客流高峰时期的弹性需求规律,进而制定了差异化的定价策略,并针对中长途客运范围内民航对高铁的影响制定合理的票价。
李旭峰,等[16]在统一计量企业以及社会属性等影响因素的条件下,制定了客运专线的客票定价体系,有助于缓解铁路客运压力。
张一腾、王小平[17]通过分析线路同一OD间的各次列车上座率,根据列车之间的相互替代性并结合交通出行乘客对于时间、价格的需求特点,在列车整体期望收益最大化为目标的约束条件下,建立了各次列车综合收益最大化的动态定价模型,从而最大限度地吸引客流,增加运输密度。
在铁路票价定价模型方面,邢泽邦,等[18]以京津城际铁路为案例对象,构建普速铁路,城际铁路以及高速铁路等运输方式的广义成本模型,并基于2012-2020年的数据对京津城际铁路各种运输方式的分担率和未来趋势进行计算和预测。
张睿, 马瑜, 赵冰茹,等[19]通过SP调查问卷的形式,详细梳理了交通出行乘客对高铁、民航的不同需求,利用Logit模型分析了高铁、民航两种交通出行方式在票价、发车频率、发车时刻等因子的变化规律,明确了高铁、民航两种交通出行方式分时段发车频率的确定方法,从而促进高铁、民航运能资源的最优配置,提高综合交通运输体系的资源利用率。
宋丹丹[20]利用系统动力学方法高铁票价的影响因素以及定价机制开展了详细研究。
galvanic replacement method -回复
galvanic replacement method -回复Galvanic Replacement Method: Unlocking the Secrets of NanotechnologyIntroduction:Nanotechnology is a field that has gained immense popularity and significance in recent years. Its applications span a wide range of industries, including electronics, medicine, energy, and materials science. One of the key techniques used in nanotechnology is the galvanic replacement method. This method has revolutionized the synthesis and fabrication of nanomaterials, leading to breakthroughs in various research areas. In this article, we will delve into the intricacies of the galvanic replacement method, exploring its step-by-step process and its impact on nanoscale science.Step 1: Understanding the BasicsTo comprehend the galvanic replacement method, we first need to understand the fundamentals. This method involves the chemical transformation of metal structures through a redox reaction. It typically occurs between two different metals, where one metal isoxidized, and the other metal is reduced. This transformation process leads to the formation of a new nanoscale structure, exhibiting unique properties that differ from those of the starting materials.Step 2: Preparation of Reactant MetalsThe first step in the galvanic replacement method is acquiring the reactant metals. These metals should have diverse properties to facilitate the redox reaction. For example, one metal could be chosen for its high reactivity towards a particular ion, while the other metal acts as a template to control the shape and size of the resulting nanomaterial. The selection of suitable reactant metals forms the foundation for a successful galvanic replacement process.Step 3: Immersion in Ionic SolutionFollowing the acquisition of reactant metals, they are immersed in a solution containing an appropriate ionic compound. The choice of this compound depends on the desired final nanomaterial and its specific properties. For instance, if one wishes to create a magneticnanomaterial, an iron salt solution may be suitable. The immersion of metals in the ionic solution initiates the redox reaction, leading to the replacement of one metal ions with the other.Step 4: Manipulating Reaction ParametersThe galvanic replacement reaction is influenced by various parameters, including temperature, pH, concentration, and reaction time. Manipulating these parameters provides control over the reaction kinetics and morphology of the resulting nanomaterial. Higher temperatures typically accelerate the reaction, while pH and concentration affect the deposition rate and the distribution of the new metal ions. These parameters need to be carefully optimized to achieve the desired nanomaterial properties.Step 5: Characterization and AnalysisOnce the galvanic replacement reaction is complete, the resulting nanomaterial needs to be characterized and analyzed. Various techniques are employed to examine its structure, composition, and properties. Electron microscopy techniques such as transmission electron microscopy (TEM) and scanning electronmicroscopy (SEM) provide high-resolution images, enabling researchers to observe the nanomaterial's morphology and size.X-ray diffraction (XRD) and energy-dispersive X-ray spectroscopy (EDS) are used to analyze the crystal structure and element composition of the nanomaterial. These characterization techniques shed light on the success of the galvanic replacement method and offer insights into potential applications.Conclusion:The galvanic replacement method is a powerful tool in the realm of nanotechnology. By harnessing the redox reaction between metals, researchers can synthesize nanomaterials with unique properties. Understanding the step-by-step process of this method, from selecting suitable reactant metals to characterizing the resulting nanomaterial, empowers scientists to unlock the secrets of nanoscale science. As advancements in nanotechnology continue to reshape various industries, the galvanic replacement method is poised to play a crucial role in the discovery and development of novel materials and technologies.。
开启片剂完整性的窗户(中英文对照)
开启片剂完整性的窗户日本东芝公司,剑桥大学摘要:由日本东芝公司和剑桥大学合作成立的公司向《医药技术》解释了FDA支持的技术如何在不损坏片剂的情况下测定其完整性。
太赫脉冲成像的一个应用是检查肠溶制剂的完整性,以确保它们在到达肠溶之前不会溶解。
关键词:片剂完整性,太赫脉冲成像。
能够检测片剂的结构完整性和化学成分而无需将它们打碎的一种技术,已经通过了概念验证阶段,正在进行法规申请。
由英国私募Teraview公司研发并且以太赫光(介于无线电波和光波之间)为基础。
该成像技术为配方研发和质量控制中的湿溶出试验提供了一个更好的选择。
该技术还可以缩短新产品的研发时间,并且根据厂商的情况,随时间推移甚至可能发展成为一个用于制药生产线的实时片剂检测系统。
TPI技术通过发射太赫射线绘制出片剂和涂层厚度的三维差异图谱,在有结构或化学变化时太赫射线被反射回。
反射脉冲的时间延迟累加成该片剂的三维图像。
该系统使用太赫发射极,采用一个机器臂捡起片剂并且使其通过太赫光束,用一个扫描仪收集反射光并且建成三维图像(见图)。
技术研发太赫技术发源于二十世纪九十年代中期13本东芝公司位于英国的东芝欧洲研究中心,该中心与剑桥大学的物理学系有着密切的联系。
日本东芝公司当时正在研究新一代的半导体,研究的副产品是发现了这些半导体实际上是太赫光非常好的发射源和检测器。
二十世纪九十年代后期,日本东芝公司授权研究小组寻求该技术可能的应用,包括成像和化学传感光谱学,并与葛兰素史克和辉瑞以及其它公司建立了关系,以探讨其在制药业的应用。
虽然早期的结果表明该技术有前景,但日本东芝公司却不愿深入研究下去,原因是此应用与日本东芝公司在消费电子行业的任何业务兴趣都没有交叉。
这一决定的结果是研究中心的首席执行官DonArnone和剑桥桥大学物理学系的教授Michael Pepper先生于2001年成立了Teraview公司一作为研究中心的子公司。
TPI imaga 2000是第一个商品化太赫成像系统,该系统经优化用于成品片剂及其核心完整性和性能的无破坏检测。
牛骨髓蛋白的酶解工艺优化及其理化性质和抗氧化特性
古丽米热·阿巴拜克日,帕尔哈提·柔孜,则拉莱·司玛依,等. 牛骨髓蛋白的酶解工艺优化及其理化性质和抗氧化特性[J]. 食品工业科技,2023,44(20):171−181. doi: 10.13386/j.issn1002-0306.2022100246ABABAIKERI Gulimire, ROZI Parhat, SEMAYI Zelalai, et al. Optimization of Enzymatic Hydrolysis of Bovine Bone Marrow Protein and Its Physicochemical and Antioxidant Properties[J]. Science and Technology of Food Industry, 2023, 44(20): 171−181. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100246· 工艺技术 ·牛骨髓蛋白的酶解工艺优化及其理化性质和抗氧化特性古丽米热·阿巴拜克日,帕尔哈提·柔孜*,则拉莱·司玛依,阿力木·阿布都艾尼,曹 博,杨晓君(新疆农业大学食品科学与药学学院,新疆乌鲁木齐 830052)摘 要:探讨牛骨髓蛋白(Bovine bone marrow protein, BBMP)酶解工艺并评价其理化性质和抗氧化活性,挖掘其潜在药用和保健功效物质基础,提升牛骨的综合利用价值。
以水解度(DH )、蛋白含量、1,1-二苯基-2-三硝基苯肼自由基(DPPH·)清除率为评价指标,结合结构表征,筛选最佳酶种。
以酶解时间、酶添加量、pH 、酶解温度为自变量,采用响应面法优化牛骨髓蛋白的酶解工艺,并研究其酶解物的理化性质和抗氧化活性。
optimal foraging 托福口语
optimal foraging 托福口语Optimal foraging theory is a concept in ecology that explores the optimal behavior of organisms when foraging for resources. It suggests that organisms will engage in behaviors that maximize their energy gain while minimizing their energy expenditure in the process of finding and obtaining food. This theory has implications for a wide range of fields, including animal behavior, ecology, and evolution.In the context of the口语part of the TOEFL exam, you might be asked to discuss the concept of optimal foraging and how it applies to a specific situation or scenario. For example, you could be asked to explain how optimal foraging theory might explain why some animals only eat certain types of food or why they prefer to forage in certain areas.To effectively respond to such a question, you would need to demonstrate a basic understanding of optimal foraging theory and its underlying principles. You would also need to demonstrate your ability to apply this knowledge to a specific situation or example.To prepare for a question like this, you could familiarize yourself with the basic principles of optimal foraging theory, including energy maximization, energy expenditure, and resource selection. You could also practice explaining how these principles might apply to real-worldexamples, such as animal behavior observations or ecological studies.Remember, the key to successfully answering this type of question is to demonstrate your ability to understand and apply basic concepts from ecology while explaining your thoughts clearly and logically.。
FuGENE HD Transfection Reagent
FuGENE ®HD Transfection Reagent1.What this Product DoesNumber of Transfection ExperimentsIn a typical experiment using HeLa or COS-1 cells, 1 ml of FuGENE ®HD Transfection Reagent can be used to perform up to three hundred transfections in 35-mm tissue-culture dishes, using 3 l of reagent combined with 1 – 2 g DNA per well. This is equivalent to over 6,000wells in a 96-well plate or 1,000 wells in a 24-well plate.L Optimal expression depends upon experimental conditions includ-ing cell type, passage history, confluence, seeding protocol, com-plex incubation time, serum batch, etc. The above amounts of reagents work well with HeLa or COS-1 cells. In other test systems,two- to three-fold higher amounts of reagent yield optimal levels of expression.FormulationFuGENE ® HD Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, sterile-filtered, and pack-aged in glass vials. It does not contain any ingredients of human or animal origin.Storage and Stability•FuGENE ® HD Transfection Reagent is shipped at +15 to +25°C.•Store FuGENE ® HD Transfection Reagent at +2 to +8°C, with the lid very tightly closed. The reagent is stable through the expiration date printed on the label when stored under these conditions.L FuGENE ® HD Transfection Reagent remains fully functional evenafter repeatedly opening the vial (at least five times over a two-month period) as long as the vial is tightly recapped and stored at +2 to +8°C between uses.L Do not store FuGENE ® HD Transfection Reagent below 0°C.Components may precipitate and alter results. If you accidently place the reagent at Ϫ20°C, briefly warm it to 37°C to dissolve any precipitate. It should function normally; however, do not return it to the freezer.Special HandlingN Always bring to room temperature and mix FuGENE ® HD Transfec-tion Reagent prior to use (vortex for one second or use inversion).N Do not aliquot FuGENE ® HD Transfection Reagent from the originalglass vials. Chemical residues in plastic vials can significantly decrease the biological activity of the reagent. Minimize the contact of undiluted FuGENE ® HD Transfection Reagent with plastic sur-faces.N Always dilute the reagent by pipetting directlyinto serum-free medium. Do not allow the FuGENE ® HD Transfection Reagent to contact the plastic walls of the tube containing the serum-free medium during the dilution step.N Do not use siliconized pipette tips or tubes.Additional Equipment and Reagents RequiredAdditional reagents and equipment required to perform transfection assays using FuGENE ® HD Transfection Reagent, but not provided,include:General Laboratory Equipment•standard cell culture equipment (e.g., biohazard hoods, incubators,microscope)•standard pipetters and micropipetters •vortex mixerFor Plasmid Preparation•purified plasmid stock (0.1 g/l – 2.0 g/l) in sterile TE (10 mM Tris, 1 mM EDTA, pH 8.0) buffer or sterile water•Genopure Plasmid Midi Kit*, Genopure Plasmid Maxi Kit*, or High Pure Plasmid Isolation Kit* can be used to prepare plasmid.For Verification of Vector Function •assay appropriate for transfected gene•G-418* or Hygromycin B* (optional; for stable transfection experi-ments)For Transfection-Complex Formation•Opti-MEM I Reduced Serum Medium, water, or serum-free medium •24-well plate to serve as test tube rack for FuGENE ® HD Transfec-tion Reagent vial•sterile polystyrene tubes or round-bottom 96-well platesCells Growing in Log Phase•select subconfluent cultures in log phase for preparation of the cell cultures for transfection•method to quantify cell number to reproducibly plate the same number of cellsApplicationFuGENE ® HD Transfection Reagent is a multi-component reagent that forms a complex with DNA, then transports the complex into animal or insect cells. Benefits of FuGENE ® HD Transfection Reagent include:•High transfection efficiency in many common cell types, including HeLa, NIH/3T3, COS-1, COS-7, CHO-K1, Hep G2, HEK-293, MCF7,and some insect cell lines. In addition, you will achieve excellent transfection efficiency in some cell lines (e.g., RAW) that are not transfected well by other reagents. Detailed transfection protocols and sample results are available at .•Demonstrates minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, and eliminates the requirement to change media after the addition of transfection complex.•Suitable for transient and stable transfection.•Functions exceptionally well in the presence or absence of serum;eliminates the need to change media.To ensure the quality of cells to be transfected, Roche recommends using freshly-obtained, low-passage cell sines form ATCC ®. For more information please visit and bookmark .For the transient and stable transfection of animal and insect cellsCat. No. 04 709 691 0010.4 ml (up to 120 transfections)Cat. No. 04 709 705 001 1 ml (up to 300 transfections)Store at +2 to +8°CCat. No. 04 709 713 001 1)Mega-pack 5 × 1 ml (up to 1,500 transfections)Cat. No. 05 061 369 00110 ml (up to 3,000 transfections)Cat. No. 04 883 560 001Trial-pack1)The five vials are packaged together in one box with one pack insertFor life science research only. Not for use in diagnostic procedures. FOR IN VITRO USE ONLY.2.How to Use this Product2.1Before you BeginRequired Amount of FuGENE® HD Transfection ReagentFor initial optimization experiments, transfect a monolayer of cells that is 80 – 90% confluent in a six-well culture dish, using 3:2, 4:2, 5:2, 6:2, 7:2, and 8:2 ratios of FuGENE® HD Transfection Reagent (l) to DNA (g), respectively. For most cell types, these FuGENE® HD Transfection Reagent:DNA ratios provide excellent transfection levels.L Subsequent optimization may further increase efficiency in your particular application. In addition to varying the volume of FuGENE® HD Transfection Reagent, other parameters may be evaluated (see section 2.6, Parameters for Optimization, and sec-tion 3, Troubleshooting).Plasmid DNA•It is critical to accurately determine the plasmid DNA concentration using 260-nm absorption (estimates of DNA content based on the intensity of gel bands are not sufficiently accurate). Determine the DNA purity using a 260 nm/280 nm ratio; the optimal ratio is 1.8.•Prepare the plasmid DNA solution in sterile TE (Tris/EDTA) buffer or sterile water at a concentration between 0.1 g/l and 2.0 g/l. Cell Culture ConditionsMinimize both intra- and inter-experimental variance in transfection efficiency by using cells that are regularly passaged, proliferating well (best when in a log-growth phase), and plated at a consistent den-sity. FuGENE® HD Transfection Reagent is different from FuGENE® 6 Transfection Reagent regarding the optimal density of cells required for maximal expression with minimal negative effect; FuGENE®6 Transfection Reagent is formulated to work at low cell densities, whereas FuGENE® HD Transfection Reagent is formulated to work at higher cell densities. Cells must be healthy and free of mycoplasma and other contaminants.L If you have used FuGENE® 6 Transfection Reagent in the past, we suggest that you increase the plating density for initial tests using FuGENE® HD Transfection Reagent. For most cell lines, use the reagent at cell-plating densities at least twice that used with FuGENE® 6 Transfection Reagent to yield maximum protein expression. For most cell lines, cultures should be 80 – 90% con-fluent at the time of transfection. For contact-inhibited cell lines such as NIH/3T3, optimal results are obtained when cells are plated at lower densities.Other Media AdditivesIn some cell types, antimicrobial agents (e.g., antibiotics and fungicides) that are commonly included in cell-culture media may adversely affect the transfection efficiency of FuGENE® HD Transfection Reagent. If possible, exclude additives for initial experi-ments. Once high-efficiency conditions have been established, these components can be added back while monitoring your transfection results. Cell growth and/or transfection efficiency may be affected by variations in sera quality or media formulations.Verification of Vector FunctionOptimize transfection conditions with a known positive-control reporter gene construct prior to transfecting cells with a new vector construct:•Determine transfection efficiency with a reporter gene assay (CAT*,-Gal*, Luciferase*, SEAP*, or hGH*).•Sequence across the flanking vector insert regions to verify the integrity of your new construct.2.2Preparation of Cells for Transfection2.3Overview of Initial Transfection ExperimentAdherent and Suspension Cells in a Six-well Plate or 35-mm Culture DishFor initial optimization, test FuGENE® HD Transfection Reagent:DNA ratios of 3:2, 4:2, 5:2, 6:2, 7:2, and 8:2 (l for FuGENE® HD Transfection Reagent, and g for DNA, respectively). The preparation of the com-plex for a single well of a six-well plate, or a 35-mm culture dish, is described in section 2.4. These ratios will function very well for com-monly used adherent cells and suspension cells. For your particular cell line and culture conditions you may find that ratios of 9:2, 10:2, 11:2, or 12:2 result in even greater expression. Try these ratios if you find the highest expression levels in the 8:2 ratio well.N Prepare the transfection complex in diluent that does not contain serum (e.g., Opti-MEM I Reduced Serum Medium), even if the cells are transfected in the presence of serum. For some cell lines, the complex may be formed in DMEM or sterile water.L For additional optimization tips, see section 2.6 and visit /fugene/hdRatio OverviewPreparation of a transfection complex that is sufficient for a 35-mm culture dish, or one well of a six-well plate, at six different ratios: Tab. 1: Preparation of transfection complex for a 35-mm culture dish.Cell Type ProcedureAdherentcellsOne day before the transfection experiment, trypsinizethe monolayer, adjust cell concentration, and plate thecells in the chosen cell-culture vessel. For most celltypes, plating 3 – 6 × 105 cells in 2 ml of medium in a35-mm culture dish (or six-well plate) overnight willachieve the desired density of Ͼ80% confluency atthe time of transfection. For cell lines with specialcharacteristics, such as contact-inhibited NIH/3T3cells, a lower plating density should be used. If usingculture plates of a different size, adjust the total num-ber of cells, starting volume of FuGENE® HD Transfec-tion Reagent, and the starting mass of DNA inproportion to the relative surface area (Table 2). SuspensioncellsUse freshly passaged cells at a concentration of 5×105/ml to 1 × 106/ml in 2 ml of medium in a 35-mmculture dish (or six-well plate). If using culture platesof a different size, adjust the total number of cells,starting volume of FuGENE® HD TransfectionReagent, and the starting mass of DNA in proportionto the relative volume (Table 2).Tubelabel(ratio)Diluent(l)FuGENE® HDTransfectionReagent (l)DNA(g)Comments3:210032Add the entire volume toa 35-mm culture dish oreach well of a six-wellplate, or 2 – 15 l to eachwell of a 96-well plate.Suggested volumes fordifferent culture vesselsare included in Table 2. 4:2100425:2100526:2100627:2100728:2100822.4Transfection ProcedureNotes:L As with any experiment, include appropriate controls. Preparewells with cells that remain untransfected, cells with transfection reagent alone, and cells with DNA alone.L For stable transfection experiments, the complex-containingmedium should be left unchanged until the cells need to be pas-saged. At that time, include the appropriate selection antibiotics (G 418* or Hygromycin B*).L To prepare transfection complexes for different-sized containers orparallel experiments, proportionally change the quantity of all components according to the total surface area of the cell culture vessel being used (Table 2).L For ease-of-use when transfecting small volumes, as in 96-wellplates containing 0.1 ml culture medium per well, prepare 100 l of transfection complex and add 2 – 15 l to each well depending upon the cell type.L The optimal ratio of transfection reagent:DNA and the optimaltotal amount of complex may vary with cell line, cell density, day of assay, and gene expressed.L After performing the optimization experiment where several ratioswere tested, select a ratio in the middle of the plateau for future experiments.2.5Cotransfection ExperimentsSuggestionsFor cotransfection experiments with FuGENE ® HD Transfection Reagent, maintain the same total reagent:total DNA ratio as that used for a single plasmid in your system. Thus, the total amount of the plas-mid DNA should be equal to the amount of plasmid used in a single plasmid transfection.ᕡAllow FuGENE ® HD Transfection Reagent, DNA, anddiluent to adjust to +15 to +25°C. Vortex for one second or invert the FuGENE ® HD Transfection Reagent vial to mix.ᕢDilute DNA with appropriate diluent, for example, Opti-MEM IReduced Serum Medium, serum-free medium (without anti-biotics or fungicides), or sterile water to a concentration of 2g plasmid DNA/100 l Opti-MEM (0.02g/l).L For insect cells, use sterile water as diluent. For other celllines, try sterile water or serum-free medium as an alterna-tive diluent.ᕣPlace 100 l diluent, containing 2 g DNA into each of six ster-ile tubes labeled 3:2, 4:2, 5:2, 6:2, 7:2, and 8:2.Recommendation : Use sterile polystyrene tubes or round-bottom, 96-well plates to form the transfection complex.L Due to manufacturer variability with release agents for96well plates, we suggest using tissue culture treated 96well plates to reduce variablity.ᕤForm the transfection complex by adding FuGENE ® HDTransfection Reagent to tubes containing diluted DNA :Pipet the FuGENE ® HD Transfection Reagent (3, 4, 5, 6, 7, or 8l) directly into the medium containing the diluted DNA with-out allowing contact with the walls of the plastic tubes.N To avoid adversely affecting transfection efficiency, do notallow undiluted FuGENE ® HD Transfection Reagent to come into contact with plastic surfaces (such as the walls of the tube that contains the serum-free medium) other than pipette tips. Do not use siliconized pipette tips or tubes.ᕥMix and incubate the transfection complex :Vigorously tap the tube or vortex for one to two seconds to mix the contents. If using a 96-well plate, place the plate on a rotat-ing shaker for 5 – 10 seconds. Incubate the transfection reagent:DNA complex for 15 minutes at room temperature.For some ratios and cell types, incubation is not necessary for optimal complex formation, while a longer incubation time is better for other cell types. Determine this for your particular cell line and the ratio you use.ᕦAdd the transfection complex to cell s:Remove culture vessel from the incubator. Removal of growth medium is not necessary. Add the transfection complex to the cells in a drop-wise manner or add below the surface of the medium. Swirl the wells or flasks to ensure distribution over the entire plate surface. Use of a rotating platform shaker for 30 seconds at low speed provides adequate mixing for 96-well plates.Once the FuGENE ® HD Transfection Reagent:DNA complex has been added to the cells, there is no need to remove and replace with fresh medium (as is necessary with some other transfection reagents).L In our experience, the exposure of most common laboratorycell types (COS-1, CHO-K1, HEK-293, HeLa, Hep G2, MCF-7) to the transfection complex until performance of the gene expression assay (24–48 hours later) does not affect the results. If you desire to transfect cells that are in serum-free medium during the transfection process, then replace the medium with serum-containing medium 3 – 8 hours after transfection, unless the cells normally grow in serum-free medium. ᕧIncubate cells and assay the results :Following transfection, incubate the cells for 18 – 72 hours prior to measuring protein expression. The length of incubation depends upon the transfected vector construct, the cell type being transfected, the cell medium, cell density, and the type of protein being expressed. After this incubation period, measure protein expression using an assay that is appropriate for your system.L If you observe low transfection levels or more than10 – 30% cell death, refer to section 3, Troubleshooting and /fugene/hd2.6Parameters for Optimization2.7Transfection of Adherent Cells Adapted for Suspension Growth•In some cases, adherent cells may be adapted for suspension growth, thus enabling the production of transiently transfected cells on a very large scale.•HEK-293 cells grown in suspension in serum-free medium that did not contain heparin or dextran sulfate produced significant amounts of protein following transfection.2.8Guidelines for Preparing FuGENE® HD Transfection Reagent:DNA Complex for Various Culture Vessel SizesThe starting volume and mass to add to the different culture vessels is based upon preparing a 100-l transfection complex as described in sec-tions 2.3 and 2.4. For best results, prepare a 100-l complex at different ratios and add varying amounts of each ratio when optimizing. The amounts below are based on the 100-l complex as prepared in sections 2.3 and 2.4.Suggested seeding density for adherent cells = 30,000 – 70,000 cells per cm2Suggested seeding density for suspension cells = 250,000 – 500,000 cells per mlTab. 2: Refer to the table below when setting up your transfection reac-tions. T hese are suggested seeding densities and are media, passage level, laboratory, and cell-line dependent. It is critical that log phase cultures are selected for subculture for the transfection experiments, and that cultures are seeded at the proper density for the transfection experiment. Observe cultures and plate them so that the monolayer is 80–90% confluent at the time of trans-fection. This must be determined empirically. For some cell lines, 60–80% con-fluency is sufficient. However, a contact-inhibited cell line, such as NIH/3T3, should be plated at lower confluence due to its growth characteristics.1) Scale up total volume for larger vessels.3.TroubleshootingParameter to beoptimizedProcedureFuGENE® HD Transfection Reagent:DNA ratio Form the transfection complex at several ratios: 3:2, 4:2, 5:2, 6:2, 7:2, 8:2, 10:2, and 12:2 (l FuGENE® HD Transfec-tion Reagent: g DNA).In some systems, altering the ratio of FuGENE® HD Transfection Reagent to DNA can increase the level of protein expression.L It has been reported that for some plasmid preparations, a ratio of 2:2 yielded optimal results. This is unusual and may reflect some property of the plasmid preparation rather than a characteristic of the FuGENE® HD Transfec-tion Reagent.Amount of transfectioncomplex addedTry adding 200%, 150%, 75%, 50%, and 25% of the amount of 100-l transfection complex suggested in Table 2.Number of cells plated Plating more cells will overcome negative growth effects of excess transfection complex. For cells with special growth characteristics, such as NIH/3T3 cells, do not use this as the first parameter for optimization.Incubation time for the transfection complex to form Vary the length of incubation time for transfection-complex formation: add the complex to the cells immediately after the components are combined and mixed, and then at several intervals up to 40 minutes (i.e., 0, 15, 25, and 40 min-utes). We have observed that in some cell lines, the transfection-complex incubation time tends to have no effect on results when using higher ratios; however, results using lower-ratio-complexes varied depending on the incubation time for complex formation.Special tips for sensitive cell lines •Reduce the time of exposure to the transfection complex (2–3 hours maximum), then replace the medium.•Use the lower ratios, and allow the complex to form for a longer period of time (determine empirically for your cell line), then add lower amounts of the complex (50% or less of what was originally tested).Culture vessel Surfacearea(cm2)TotalvolumeofmediumSuggested seeding densitySuggested amount ofthe 100-l trans-fection complex toadd to each well (l)Final amount of FuGENE® HDTransfection Reagent (l) ineach well following addition ofsuggested amount of 100-ltransfection complexCells/wellAdherent cellsCells/wellSmall or suspensioncellsUsing the3:2 ratioUsing the8:2 ratio totalvolumevolume forlargerlow high low high96-well plate(1 well)0.30.110,00020,00025,00050,00050.150.424-well plate(1 well)1.90.550,000125,000250,000500,000250.752.012-well plate(1 well)3.8 1.0100,000250,000375,000750,00050 1.54.0 35-mm dish82200,000500,000500,0001,000,000100 3.08.06-well plate(1 well)9.42200,000600,000500,0001,000,000100 3.08.0 60-mm dish215500,0001,400,0001,250,0002,500,000250 1)7.520.0 10-cm dish55101,500,0003,500,0002,500,0005,000,000500 1)15.040.0 T-25 flask256700,0001,700,0001,500,0003,000,000300 1)9.024.0 T-75 flask75202,000,0005,000,0005,000,00010,000,000900 1)27.072.0Low trans-fection efficiency Poor quality orinsufficient quantityof nucleic acidsVerify the amount, purity, and sequence of nucleic acid.Perform a control transfection experiment with a commercially available transfection-grade plasmidpreparation.Chemical contaminants may be in the plasmid preparation. Avoid phosphate buffers until you havetested them in your system.L Endotoxins are reported to be cytotoxic to some very sensitive cell lines.Insufficient numberof cellsUse adherent cells that are at least 80% confluent. Low cell density results in fewer cells available totake up transfection complex, and excess complex may be cytotoxic; in addition, fewer cells yieldless protein.Too many cells orcells post log phaseWhen confluent cultures are subcultured, or cells are plated at too high a density, the cells fail to dividein the culture being transfected. This results in suboptimal expression.Suboptimal FuGENE®HD TransfectionReagent:DNA ratio,complex incubationtime, total amount oftransfection complexadded, or cell densityOptimize the FuGENE® HD Transfection Reagent:DNA ratio, complex incubation time, amount ofcomplex added to cells, and cell density, according to the following procedure:Day before transfection:Prepare two 96-well plates of cells at high and low seeding densities (see Table 2 for suggestions).Day of transfection:•Form 200 l of transfection complex at ratios of 2:2, 3:2, 4:2, 5:2, 6:2. 7:2, and 8:2 (l transfectionreagent:g DNA) following the protocol in this pack insert (sections 2.3, 2.4) and doubling theamounts of all components.•As soon as the complexes are combined and mixed, add 10, 5, or 2.5 l of each complex to one of3columns of cells in each 96-well plate (i.e., columns 2, 3, and 4). Leave all outer wells empty ascontrols.•Continue to incubate the complexes at room temperature. After an additional 10 – 15 minutes, add 10,5, or 2.5 l of each complex to the next 3 columns (5, 6, and 7) of cells in each 96-well plate.•Continue to incubate the complexes at room temperature. After an additional 10 – 15 minutes, add 10,5, or 2.5 l of each complex to the next 3 columns (8, 9, and 10) of cells in each 96-well plate.•Assay the plates 1–2 days later. Select the ratio, amount of complex, and time of transfection-complexincubation that resulted in optimal expression.•If optimal transfection occurs at the higher ratios, repeat this process using ratios of 6:2, 7:2, 8:2, 10:2,12:2, and 14:2. Add 5, 10, and 15 l of complex. We have never successfully transfected cells using theratio of 2:2, but it has been reported that some plasmid preparations transfect at this ratio.See section 2.6, Optimization of FuGENE® HD Transfection Reagent:DNA ratio, for more information andvisit /fugene/hdFuGENE® HD Trans-fection Reagent wasaliquotedCheck that FuGENE® HD Transfection Reagent is stored in the original container. If the reagent wasaliquoted into plastic containers, there is a high chance of inactivation. Make sure the reagent isimmediately mixed with the dilute DNA either by vortexing or pipetting up to 10 – 15 times.FuGENE® HD Trans-fection Reagent cameinto contact withplastic or wasinadequately mixedRepeat transfection, carefully pipetting FuGENE® HD Transfection Reagent directly into the serum-freemedium, being careful not to touch the sides of the container while adding the FuGENE® HD Transfec-tion Reagent to the diluted DNA. If the FuGENE® HD Transfection Reagent is added too gently, it maylayer on top of the medium, thus making contact with the plastic.Transfection complexwas formed in serum-containing mediumCheck original bottle of medium used for complex formation. Repeat experiment using new bottle ofOpti-MEM that does not contain any additives (e.g., serum, antibiotics, growth enhancers, heparin,dextran sulfate, etc.). Try forming the complex in sterile water or plain DMEM.Media and mediacomponentsDifferent media and media components may influence the level of transfection efficiency andsubsequent growth of the transfected cells, as well as expression of the recombinant protein. Some lotsof sera have been reported to interfere with optimal transfection.Quality and/or lot-to-lot differences that affect transfection experiments have been noted in both seraand media. Check that the medium and/or serum is from the same lot that worked previously. Try newlots or a different vendor.Culture may becontaminated withmycoplasmaCultures contaminated with mycoplasma have been shown to have decreased transfection efficacy.Determine if culture is contaminated with mycoplasma; use the Mycoplasma Detection Kit* orMycoplasma PCR ELISA* to assess contamination.Inconsistent results Ratio or amount oftransfection complexis at the edge ofperformance plateauInitial experiments should be completed to determine the ratios, amount of complex to be added, andlength of time for complex formation for optimal performance. In our experience, we have found the pla-teau to be relatively broad. We recommend that future experiments be performed with ratios, incubationtime, and amounts of complex that were in the middle of the plateau. If conditions are selected at theedge of the plateau, very small procedural differences may cause large differences in the resulting pro-tein expression. Increased consistency may be achieved by shifting parameters away from the edge ofthe plateau to the middle of the plateau.Transfection complexformation:timing,amounts, and ratioFormation of the complex involves a multifaceted interaction between the transfection reagent and DNAas well as biological parameters. Differences in any of the components or techniques may result ininconsistencies. If results do not meet your expectations, then repeat the optimization experimentselecting areas near the plateau found in previous experiments. For current experiments, determine ifyou should use a different ratio, length of time, or amount of complex for more consistent transfectionresults.Extensive testing of the FuGENE® HD Transfection Reagent is performed on two cell lines: one easy totransfect and one very difficult to transfect. All reagent lots must pass this rigorous testing before wemake it available to you. However, we cannot test all cell lines, media, sera, and vectors; in your labora-tory, you may find slight differences in the optimal ratio, amount of complex, or time for complex forma-tion for some lots of FuGENE® HD Transfection Reagent.Cells For consistent results, cells must be properly maintained. Cells change with passage level, passage conditions, media, and sera. For some cell lines, these changes have little to no effect on transfectionexperiments, but for other cell lines, these changes have profound effects. Each cell type may have a dif-ferent optimal transfection condition. Optimal values for a single cell type may also change slightly withvector construct and type of protein expressed.Observation Possible cause RecommendationSigns of cytotoxicity Transfected protein iscytotoxic or isproduced at highlevelsReduced viability or slow growth rates may be the result of high levels of protein expression, as the cell’smetabolic resources are directed toward production of the heterologous protein. The expressed proteinmay also be toxic to the cell at the level expressed.To analyze cytotoxicity, prepare experimental controls as described below.Prepare extra control wells containing:ቢ Cells that are not transfectedባ Cells treated with DNA alone (e.g., without FuGENE® HD Transfection Reagent)ቤ Cells treated with FuGENE® HD Transfection Reagent alone (no DNA added)ብ Cells transfected with a non-toxic or secreted protein.Compare experimental transfected cells to cells in the control wells (described above). Considerrepeating the experiment with a secreted reporter gene such as SEAP, hGH, or a standard -gal controlvector. Cells expressing SEAP should show little to no evidence of cytotoxicity.Too much transfec-tion complex fornumber of cellsIncrease the number of cells plated, and/or decrease the total amount of complex added to the cells. Trydifferent ratios and allow the complexes to form for different time intervals. Add different amounts ofcomplex; for example, make the complex as usual but add 75%, 50%, or 25% of the usual amounts toeach well. See Suboptimal FuGENE® HD:DNA Ratio in "Low transfection efficiency" section of this tablefor details or optimization protocol.Culture may becontaminated withmycoplasmaDetermine if culture is contaminated with mycoplasma; use the Mycoplasma Detection Kit* orMycoplasma PCR ELISA* to assess contamination.Cells may not behealthyAssess physiological state of cells and the incubation conditions (e.g., check incubator CO2, humidity,and temperature levels). Observe cells prior to each passage for morphology and absence of contami-nants. Make sure cells do not overgrow. Routinely passage cells prior to reaching confluency. Make surethat culture media and additives are within expiration date and have been stored properly.Diluent is toxic to thecellsDMEM is toxic to some insect cell lines. For these cells, prepare the transfection complex in sterilewater. You may also try forming the complex in the medium in which the cells are growing, providingthat the medium does not contain serum, heparin, or dextran sulfate.Plasmid preparationcontaminated withendotoxinEndotoxin is reported to be cytotoxic to sensitive cell lines.If above tests provenegative, FuGENE®HD TransfectionReagent may not beoptimal for your cells.Try FuGENE® 6 Transfection Reagent*, DOTAP Liposomal Transfection Reagent*, DOSPER LiposomalTransfection Reagent*, or X-tremeGENE Q2 Transfection Reagent*.High protein-expression levelsHigh expression levels of certain intracellular proteins (e.g., Green Fluorescent Protein [GFP]) may becytotoxic to some cell types. Cell proliferation, toxicity, and cell death may be monitored using Apoptosisand Cell Proliferation products from Roche Applied Science (visit /apoptosis for more information).Media and mediacomponentsTest different media and optimize the level of each medium component for these cytotoxic effects.Although it is not usually necessary to remove the transfection complex following the transfection step,it may be necessary to feed your cells with fresh media for extended growth periods. This is particularlyimportant if the transfected cells are allowed to continue to grow for 3 – 7 days to provide maximalprotein expression.。
关于进一步推进电能替代的指导意见英语
关于进一步推进电能替代的指导意见英语Guidelines for Promoting Further Substitution of Electric PowerIn recent years, the substitution of electric power has become an increasingly significant issue. As the demand for energy grows and environmental concerns escalate, it is crucial to explore and implement effective measures to encourage the substitution of electric power. This document aims to provide guidance for promoting further substitution of electric power, focusing on innovative strategies and practical solutions.I. BackgroundElectric power substitution refers to the replacement of traditional energy sources with electricity in various sectors, such as transportation, heating, and industrial processes. This substitution plays a vital role in achieving energy conservation and emission reduction goals while ensuring sustainable development. Therefore, it is imperative to formulate effective strategies to accelerate the progress of electric power substitution.II. Rationale for Further Electric Power Substitution1. Energy Conservation: Electric power substitution significantly contributes to energy conservation efforts by reducing reliance on fossil fuels. By utilizing electricity as a substitute, energy efficiency can be greatly improved, resulting in reduced consumption and overall energy savings.2. Environmental Benefits: The substitution of electric power helps mitigate environmental pollution caused by traditional energy sources. Electricity, particularlygenerated from renewable energy, substantially reduces greenhouse gas emissions and air pollutants, leading to a cleaner and healthier environment.3. Technological Advancements: Rapid advancements in electric power technologies have made it more feasible and cost-effective to substitute electricity for traditional energy sources. Innovations in battery storage, electric vehicles, and energy-efficient appliances pave the way for accelerated adoption and successful implementation of electric power substitution.III. Strategies for Promoting Further Electric Power Substitution1. Policy Support: Governments should formulate comprehensive and supportive policies to encourage the substitution of electric power. This includes implementing favorable regulations, providing financial incentives, and establishing research and development programs to foster technological innovation in the electric power sector.2. Infrastructure Development: Invest in the development of robust electric power infrastructure, including charging stations, grid expansion, and smart grid technologies. Enhancing the accessibility and availability of electric power infrastructure is crucial for facilitating the widespread adoption of electric vehicles and other electric-powered devices.3. Public Awareness and Education: Conduct public awareness campaigns to educate the general public about the benefits of electric power substitution and its role in sustainable development. Promote the use of electric-powered devices, publicize success stories, and emphasize the long-term benefits of transitioning to electric power.4. Technological Collaboration: Foster collaboration between industry leaders, research institutions, and academia to accelerate technological advancements in electric power substitution. Encourage joint research projects, knowledge sharing, and technological collaborations to expedite the development and commercialization of innovative electric power solutions.IV. Evaluation and MonitoringEstablish a comprehensive evaluation and monitoring system to assess the progress and impact of electric power substitution initiatives. Regularly monitor key performance indicators, such as energy consumption reduction, emission reduction, and cost-effectiveness. Based on the evaluation results, make necessary adjustments and refine strategies to ensure the continuous improvement of electric power substitution efforts.ConclusionThe substitution of electric power holds tremendous potential in addressing energy and environmental challenges. By embracing innovative strategies and implementing practical measures, we can further accelerate the adoption of electric power and create a sustainable and greener future. Together, let us work towards a society where electric power substitution becomes the norm rather than the exception.。
英语栅格重采样 -回复
英语栅格重采样-回复the following question: [英语栅格重采样]Resampling in English Raster Grids: A Step-by-Step GuideIntroduction:Resampling is an important technique in the field of remote sensing and GIS (Geographic Information Systems) that allows for the transformation and adjustment of raster data. Particularly in the case of English raster grids, resampling plays a vital role in ensuring the accuracy and precision of spatial analysis. This article aims to provide a step-by-step guide on how to perform resampling in English raster grids, detailing the process and its significance.Step 1: Preparing the DataBefore diving into the resampling process, it is crucial to ensure that all necessary data is correctly formatted and organized. The first step involves gathering and importing the English raster grid dataset into the preferred GIS software. Make sure that the dataset aligns with the intended analysis and carries the appropriateattribute information.Step 2: Defining the Projected Coordinate SystemTo accurately resample an English raster grid, it is essential to define the projected coordinate system within the GIS software. This system identifies the spatial reference of the dataset and ensures correct alignment during the resampling process. Selecting an appropriate projected coordinate system is critical for preserving the integrity and accuracy of the resulting raster grid.Step 3: Determining the Desired Cell SizeResampling involves adjusting the cell size of the raster grid. In this step, it is necessary to determine the desired cell size based on the analysis requirements. Consider the level of detail needed and the overall objective of the study. A smaller cell size provides higher resolution but may increase computational load, while a larger cell size sacrifices details but reduces processing time.Step 4: Selecting the Resampling TechniqueVarious resampling techniques exist, each with its characteristics and implications. Popular methods include nearest neighbor, bilinear interpolation, and cubic convolution, among others. Evaluate the advantages and disadvantages of each method based on the specific dataset and analysis requirements. Generally, the nearest neighbor method is suitable for categorical data, while bilinear or cubic interpolation is more suitable for continuous data.Step 5: Executing the Resampling ProcessOnce the preparatory steps are completed, it is time to execute the resampling process. This typically involves accessing the resampling tool or function within the GIS software. Specify the desired cell size determined in Step 3 and select the appropriate resampling technique decided in Step 4. The software will then perform the transformation and generate the resampled English raster grid.Step 6: Evaluating the ResultsAfter the resampling process, it is important to evaluate the quality of the newly generated raster grid. Compare it with the originalgrid and assess if the desired outcomes and objectives were achieved. Pay attention to potential changes in spatial patterns, attribute values, and overall accuracy. Should any discrepancies arise, consider re-evaluating the initial data or adjusting the resampling technique used.Conclusion:Resampling in English raster grids is a fundamental process in remote sensing and GIS analysis. This guide has provided astep-by-step explanation of the procedure, outlining the essential considerations and decision-making points. By following these steps, analysts can confidently resample data, ensuring accurate and precise results for various spatial analyses.。
红枣山楂果酒的酿造工艺优化
2021年第40卷第4期总第350期 ・203・中国酿造产品开发红枣山楂果酒的酿造工艺优化姜兴旭,张阳阳,朱 静*,王荣荣,张永耀(信阳农林学院(河南 信阳464000)摘要:该试验选择红枣和山楂为原料酿造果酒,以原料配比、料水比、初始糖度和酵母添加量为评价因素进行单因素试验,在此基础上采用正交试验进行发酵工艺优化。
结果表明,果酒酿造的最佳工艺条件为红枣'楂配比3:1,料水比l :3(g :mL ),初始糖度21 "B l , 酵母添加量0.3 3L ,在2,七发酵7 d 。
在此条件下所制得的红枣'楂果酒酒精度为12.2%ro 1,总黄酮含量为25 mg/mL 。
酒液枣红色,色 ,澄清透明,有 的红枣山楂的 ,酒 ,是一种酸甜适中的低酒精度的 酒。
关键词:功能红枣;'楂中图分类号:TS262.7 文章编号:0254-5071 (2021)04-0203-04 doi:10.11882/j.issn.0254-5071.2021.04.039引文格式:姜兴旭,张阳阳,朱静,等•红枣'楂果酒的酿造工艺优化[J].中国酿造,2021,40(4): 203-206.Optimization of brewing process of j ujube-hawthorn fruit wineJIANG Xingxu, ZHANG Yangyang, ZHU Jing*, WANG Rongrong, ZHANG Yongyao(Xinyang Agriculture and Forestry College, Xinyang 464000, China)Abstract : Using jujube and hawthorn as raw materials to brew fruit wine, the raw material ratio, liquid-solid ratio, initial sugar content, yeast addition wasoptimized by single-factor tests. On this basis, the fermentation conditions were optimized by orthogonal experiments. Results showed that the optimalbrewing conditions were jujube and hawthorn ratio 3:1, liquid-solid ratio 1 :3(g :ml), initial sugar degree 21 "Bx, yeast addition 0.3 g/L, fermentation tem perature 28 # for 7 d. Under the conditions, the alcohol content of j ujube hawthorn wine was 12.2% v ol, and the total flavonoids content was 25 mg/ml. The wine was jujube red, bright in color, clear and transparent, with strong jujube and hawthorn aroma and full-bodied. It was a compound fruit wine with low alcohol content and moderate sweet and sour taste.Key words : functionality; jujube; hawthorn; brewing process山楂含酸较多,能开胃健脾,且富含黄酮类物质,具 有滋阴补阳、补气养血等功效,对心血管系统有明显的药理作用,是常见的果酒原料冋。
三角帆蚌耗氧率和排氨率的昼夜变化及不同饵料浓度下的摄食节律
Vol.33,No.6Dec. 2020第33卷第6期2020年12月水产学杂志CHINESE JOURNAL OF FISHERIES文章编号:1005-3832( 2020 )06-0056-05三角帆蚌耗氧率和排氨率的昼夜变化及不同饵料浓度下的摄食节律孙明龙巴白志毅",傅百成3,何志然",郭思鹏1,2(1.上海海洋大学农业农村部淡水水产种质资源重点实验室,上海201306;2上海海洋大学,上海水产养殖工程技术研究中心,上海201306;3.浙江佰瑞拉农业科技公司,浙江绍兴311800)摘要:在水温271条件下,将体质量为(1&43 ± 4.21 )g 的一龄三角帆蚌Hyriopsis cumingii 放入20 L 塑料桶内暂养1周后,采用室内静水呼吸法测定了蚌的耗氧率和排氨率昼夜变化;并设置了 4种小球藻Chlorella S p.投喂初始浓度,分别为 1.9 x 109 ceUs-L-\3.25 x 109 ceUs-L-\4.11 x 109 ceUs-L-1 和 5.05 x 109 ceUs-L-1,测定了三角帆蚌的日摄食节律和摄食量。
结果表明,三角帆蚌的耗氧率和排氨率每日呈现两个代谢周期,代谢最低谷出现 在中午12点,然后逐渐提升,至夜间20点出现第一次代谢高峰,然后逐步下降,至凌晨0-2点之间处于第二 个代谢低谷期,凌晨4点出现第二次代谢高峰,并发现三角帆蚌夜间耗氧率和排氨率总体水平明显高于白天。
另外,观察到三角帆蚌日摄食量受到饵料浓度的影响,小球藻浓度为4.11 x lO^ceU-L-1时,三角帆蚌的日摄食总量最高。
本研究结果可为三角帆蚌工厂化养殖下的饵料投喂等养殖管理提供参考。
关键词:三角帆蚌;耗氧率;排氨率;摄食节律;日摄食量中图分类号:S966.22+1 文献标志码:ADiurnal Variation in Oxygen Consumption and Ammonia Excretion and Feeding Rhythm ofFreshwater Mussel Hyriopsis cumingii Under Different Alga ConcentrationsSUN Minglong", BAI Zhiyi", FU Baicheng 3, HE Zhiran*, GUO Sipeng"(1. Key Laboratory of F reshwater Aquatic Germplasm Resources, Ministry of A griculture and Rural Afiairs, Shanghai OceanUniversity, Shanghai 201306, China;2. Shanghai Aquaculture Engineering Technology Research Center, Shanghai Ocean University, Shanghai 201306, China;3. Zhejiang Bairila Agricultural Technology Company, Shaoxing 311800, China)Abstract: Hold one-year-old freshwater mussel Hyriopsis cumingii with body weight of (18.43 ± 4.21) g in 20 L plastic buckets at water temperature of 27 °C for one week of r elaying. Then diurnal variation in oxygen consumption rate and ammonia excretion rate of freshwater mussel were measured by indoor still water breathing method. The four initial feeding concentrations of chlorella sp. wereset to 1.9 x 109 cells •L 1, 3.25 x 109 cells •L 1, 4.11 x 109 cells • L 1 and 5.05 x 109 cells • L _1, and then the daily feeding rhythm and food intake of freshwater mussel were measured. The results showed that the oxygen consumption rate and ammonia excretion rate offreshwater mussel presented two metabolic cycles per day. The first trough of metabolism appeared at 12:00 at noon, and then it gradually raised to 20:00 pm, which time was the first metabolic peak appeared. After that, it gradually declined until the secondmetabolic trough appeared which was between 0:00 and 2:00 at night, and the second metabolic peak appeared at 4:00 am. It was found that there was significant diurnal variation in the oxygen consumption rate and ammonia excretion rate in freshwater mussel,significantly higher at night than those in daytime. In addition, it was observed that the daily food intake of freshwater mussel was affected by the concentration of bait feed, and the maximal daily total food intake was observed in freshwater mussel fed the alga at a concentration of 4.11 x 109 cell* L _1.The findings help to guide the culture management of feeding pattern of the freshwater mussel un der industrial culture conditions.Key words: Hyriopsis cumingii; oxygen consumption rate; ammonia excretion rate; feeding rhythm; daily food intake收稿日期:2019-09-18基金项目:现代农业产业技术体系建设专项(CARS-49);上海市崇明区“可持续发展科技创新行动计划” (CK2018- 34).作者简介:孙明龙(1994-),男,硕士研究生,从事淡水贝类养殖和种苗工程.E-mail: ****************通信作者:白志毅,男,教授,博士研究生导师.E-mail: **************.cn6期孙明龙等:三角帆蚌耗氧率和排氨率的昼夜变化及不同饵料浓度下的摄食节律•57-耗氧率和排氨率是衡量动物生理代谢的重要指标,滤食性贝类摄食和这方面的生理代谢已有较多研究W海水贝类,如青蛤Cyclina sinesis、华贵栉孔扇贝Chlamys nob ills和马氏珠母贝Pinctada martensii等工厂化人工育苗技术较成熟,已达到规模化和产业化养殖,并且这些贝类摄食和代谢生理研究也较多,而淡水贝类的耗氧率、排氨率和摄食节律研究还较少。
BlackSoldierFly,WhiteMagic-TheBioPod
skins, to allow for their rapid growth, in Array five successive phases called instars. Under ideal conditions, the larvae mature in 10 days. Their tissues are now developed enough, and they have stored energy reserves enough, to support the next phase: the miracle of metamorpho-sis, the transformation into a completely different insect form. Pupation does not take place within the feeding medium, however. When they are ready, the prepu-pal grubs have the instinct to leave it and find a place to burrow into the earth and initiate metamorphosis. After ten days or so, they emerge as winged adults.As is the case with many species of but-terfly, the adult phase is exclusively sexual: The winged phase is solely about mating, and, for the female, finding the best pos-sible place to lay her eggs. They do not feed at all in this phase, which lasts only five to eight days. Death quickly ensues for both male and female adults, once fertile eggs have been laid to start the cycle anew.An Alliance with the Soldier Useful ideas emerge from alert obser-vation. In the case of the Black Soldier life cycle, we should note particularly: They are specialists in a critically important role—the breakdown of organic debris that would otherwise choke the ecology. Their high level of feeding activity and rapid growth in the larval stage imply the concentration of considerable nutrients. Since the adults do not feed—indeed, they do not even have functioning mouth parts—they do not bite, nor do they come buzzing around us or our houses, looking for something to eat. Of special interest is their habit of crawling out of the feeding medium when it is time to pupate. These facets of the life cycle hint at an opportunity to make an alliance with Hermetia illucens to: responsibly manage organic residues such as spoiled or unused food, manures, culled fruits and vegetables, etc.; and to reclaim the residual energy in such materials as addi-tions to soil fertility, or even high quality feed (the mature grubs themselves) which we can offer to livestock species such as chickens, pigs, or farmed fish. By chan-neling the “crawl-off” of the grubs, we can even cause them to self-harvest into a collection bucket! And because the vigorous activity of the larvae keeps the feeding medium constantly aerated (most pathogenic microbes prefer anaerobicconditions), and because the adults do notfeed at all, this species is not a vector for diseases. Happily, soldier grubs tend to inhibit development of larvae of all other fly species—house flies, fruit flies, blow flies—so cultivating the Black Soldier can actually reduce populations of flies with a higher “nuisance profile.”Much creative work has in fact been done toward making exactly such an alliance. Dr. Paul Olivier has designed systems for grub composting in Viet-nam and other developing countries, to reduce the load of food wastes on streets and in landfills, while providing needed employment. Academic researchers such as Sophie St-Hilaire and Craig Sheppard have experimented with soldier grubs to manage fish offal, and manures in com-mercial poultry and swine houses; and to yield high-protein feed supplements for various livestock species, including commercially raised carnivorous fish. Dr. Olivier and his son Robert have founded a company (ESR International, LLC) which sells the well designed BioPod™, a molded high-density plastic bin similar in some ways to, but more efficient than, home scale “worms eat my garbage” vermicomposting units. Parameters for Black SoldierCompostingI am working a soldier grub colony in a BioPod™, and in a following article will detail its management; as well as explore some ideas for more low-tech, less expensive, do-it-yourself projects for soldier grub composting. My goal is to replace 25% of purchased feed I offer my chickens with nutrient-dense soldier grubs (42% protein, 35% fat, dry weight). For now, these are some of the param-eters that govern the design and manage-ment of a successful operation.RangeIf you live in climate zones 7 through 10, there is almost certainly a native Black Soldier population ready to work for you. (I’m in Zone 6b, and I found soldier grubs in my vermicomposting bins for years before I started cultivating them.) All you have to do to start a work-ing colony is to set up feeds and protected conditions that fit their life cycle; and the gravid females (mated, ready to lay eggs) will come. Soldiers can survive consider-ably farther north than Zone 7. If there is no wild population, you can purchase “starter” grubs through the mail. How easy it will be to keep a local populationthe BioPod™ shows us a component that is essential,to “ramp out” of the feeding medium when it is time topupate. The ramp here is molded into the body of the BioPod™ itself. It ends at the opening into a plastic tube, through which the migrating grubs free-fall into the liddedand next morning HarveyFor More Information onthe Black Soldier• : Ablog maintained by “Jerry aka GW,”who has been cultivating soldier grubsto feed pond fish for several years. GWalso serves as a distributor of the welldesigned BioPod™, which I am cur-rently using to house my grub colony.Quoting GW: “If you like gardening,traditional composting, or vermicul-ture, then you’re a likely candidate forblack soldier fly culturing. Likewise,if you watch nature and science showsyou will probably find BSF as fascinat-ing as I do.”• : Thewebsite for ESR International, LLC,the company that designed and sellsthe BioPod™ for soldier grub com-posting. Lots of useful informationabout managing a working colony,and about many other bioconversion/waste management issues. (BioPod™is a trademark of ESR International,LLC.)• /forum:An online discussion forum, sponsoredby ESR International, LLC, for enthu-siasts working with this fascinatingspecies.• /pages/bio-conversion.html: Background to thedevelopment of the major BioPod™designs to date.• http://aggie-horticulture.tamu.edu/galveston/beneficials/beneficial-51_black_soldier_fly.htm: One-pagesummary of facts about Hermetia il-lucens and what it has to offer.• //wp-content/uploads/2009/05/Biocon-version-Paul-Olivier.pdf: An onlineversion of a 99-slide presentation on theBlack Soldier Fly and other bioconver-sion issues.•/waste_mgt/smithfield_projects/phase2r-eport05/cd,web%20files/A2.pdf: Re-port of North Carolina State Universitystudy of conversion of swine manureusing soldier grubs.•h t t p://w w w.n a b u u r.c o m/f i l e s/a t t a c h/2008/07/task/11281_46dfe83eeb5ff.pdf: De-tailed proposal for solving waste man-agement problems in Vietnam, whileproviding or enhancing thousands ofjobs, centered on soldier grub conver-sion of organic compostables. Mature soldier grubs (prepupae)—about 3/4 inch long, flat, and segmented—have skinHer favorite meal.America’s Oldest Bird Supply Company! Automatic DoorkeeperWhat a great product! Used in Europe for years. Foy’s is proud to be your distributor in North America. Foy’s truly believes that many different uses will be found for this Electronic Doorkeeper. Cat and dog owners may use it to open and close an entrance to the dog house, garage or out building. Poultry fanciers may want to use it to allow the flock to go outside and then close it when it gets dark. Pigeon fanciers will be able to allow a door to open at dawn and close it at a chosen time later. Pigeon racers may use it when flying the darkening system, or perhaps if you have a special use, you can let us know. This automatic control device can be used wherever a vertical sliding gate is to be opened and close to respond to light onditions at dawn and dusk. The sensitivity of the system is adjustable. Using one of our timers, it is not only possible to open and close the sliding gate according to the time settingor to open and close it in response to light conditions, but it is also easyto set a maximum for the closing time.Other uses for the Electronic Doorkeeper is poultry houses, dog and cat entrances, etc. Perhaps you want an animal to be released at a certain time, after you leave for work for example. Just set the time and it will open. You can close it at your convenience or set a time for it to closeVisit his website at www.themodernhome-Front:Back:Backyard Poultry T-Shirt Order FormAll T-shirts are $14.00 each and available in red, navy blue or white.O RDER Y OURS T ODAY!Tell the world how you really feel...Available in three colors, and two styles.O n ly $14。
响应面法优化Fenton预处理精细化工废水
类、不饱和醛类和芳香类化合物. 研究显示ꎬFenton 预处理能有效降解废水中难降解有机物ꎬ但出水仍未达到后续生化处理要求ꎬ
还需进一步优化或与其他预处理工艺组合.
关键词: Fenton 氧化法ꎻ 精细化工废水ꎻ 响应面ꎻ 反应动力学ꎻ 光谱分析
中图分类号: X703 文章编号: 1001 ̄6929(2019)08 ̄1419 ̄08
Abstract: The wastewater from a fine chemistry enterprise in Gansu Province is characterized by complex ingredientsꎬ high organic matter contentꎬ and poor biodegradability. In order to meet the requirements of subsequent biochemical processesꎬ it is essential to carry out appropriate pretreatment technology research. The present study demonstrated a trial of optimizing Fenton oxidation process for pretreatment of the fine chemistry wastewater via the response surface methodology ( RSM) . Based on the results of the single factor experimentꎬ the initial pHꎬ H2 O2 dosageꎬ n( H2 O2 ) ∶n( Fe2+ ) and reaction time were selected as the investigation factors and the CODCr removal efficiency as the response value. The individual effect of the four independent factors and the synergistic effects of their interactions on CODCr removal were studied and analyzed. Moreoverꎬ the apparent reaction kineticsꎬ structural changes of wastewater organic matterꎬ and the degradation mechanisms were studied by ultraviolet spectroscopy and Fourier transform infrared spectroscopy. The results showed that: ( 1) The optimum process conditions were initial pH of 4ꎬ H2 O2 dosage of 8 mL∕Lꎬ n( H2 O2 ) ∶n( Fe2+ ) of 12ꎬ reaction time of 88 minꎬ and CODCr removal efficiency of 30������ 15%ꎬ which fitted well with the experimental results. (2) The degradation pathways of the organic matter in the fine chemistry wastewater by Fenton oxidation were complicatedꎬ and it′s difficult to fit through a single substrate model. ( 3) Fenton oxidation could effectively degrade unsaturated organic matter in the wastewater. Howeverꎬ the effluent still contained amidesꎬ unsaturated aldehydesꎬ and aromatic compounds. In conclusionꎬ Fenton oxidation pretreatment can effectively degrade the refractory organics in wastewater. Howeverꎬ the effluent still does not meet requirements of the subsequent biochemical treatment. Thusꎬ further optimization or combination with other pretreatment processes is required. Keywords: Fenton oxidation processꎻ fine chemical wastewaterꎻ response surface modelꎻ reaction kineticsꎻ spectral analysis
人工光合作用英语
The Artificial Photosynthesis: A Revolution in Renewable EnergyIntroduction:In recent years, there has been an increasing concern about the world’s depleting fossil fuel reserves and the harmful environmental impacts of their usage. As a result, the search for sustainable and renewable energy sources has gained paramount importance. One of the most promising advancements in this field is the concept of artificial photosynthesis.Artificial Photosynthesis: Unveiling the Concept:Photosynthesis, the process by which plants convert sunlight into energy, has long been admired by scientists for its efficiency and sustainability. Artificial photosynthesis aims to replicate this natural process, but in a controlled environment, to generate clean and renewable energy. The driving force behind this concept is to unlock the potential of sunlight as a virtually limitless energy source.How Does Artificial Photosynthesis Work?At its core, artificial photosynthesis involves using a photoelectrochemical cell to split water molecules into hydrogen (H2) and oxygen (O2) using sunlight as the source of energy. The hydrogen gas produced can then be utilized as a clean fuel for various applications, such as powering vehicles or generating electricity.Key Components of an Artificial Photosynthesis System:1.Photovoltaic Panel: Similar to solar panels used in conventional solarenergy systems, photovoltaic panels in an artificial photosynthesis setupcapture sunlight and convert it into electricity. This electricity is then used to power the artificial photosynthesis system.2.Catalyst: A catalyst is used to accelerate the rate of the chemicalreactions involved in the splitting of water molecules. These catalysts facilitate the process, making it more efficient and reducing energy losses.3.Electrolyzer: An electrolyzer acts as the reaction chamber wherewater molecules are split into hydrogen and oxygen gases. It consists of two electrodes, an anode, and a cathode, submerged in an electrolyte solution.When an electric current is applied, water molecules are dissociated, resulting in the production of hydrogen and oxygen gases.Advantages of Artificial Photosynthesis:1.Renewable Energy Source: Artificial photosynthesis harnesses thepower of sunlight, making it an essentially inexhaustible and renewable energy source. Unlike fossil fuels, which deplete over time, sunlight will continue to shine for billions of years.2.Capacity for Storage: Unlike other renewable sources like wind orsolar energy, artificial photosynthesis has the potential for scalable andefficient energy storage. The generated hydrogen gas can be stored and utilized when needed, making it a reliable source of energy.3.Carbon Neutrality: The utilization of hydrogen gas produced byartificial photosynthesis as a fuel does not emit greenhouse gases, making it a carbon-neutral energy solution. This could significantly reduce the carbonfootprint associated with traditional energy sources, thus combating climate change.Challenges and Future Prospects:While artificial photosynthesis holds immense promise, several challenges need to be overcome for its widespread implementation. The efficiency of the system needs to be improved, and the costs associated with catalysts and photovoltaic materials should be reduced. Additionally, the development of large-scale and commercially viable artificial photosynthesis systems is still in the early stages.Conclusion:Artificial photosynthesis is a revolutionary concept that has the potential to transform our energy landscape. By utilizing the power of sunlight, this technology offers a clean, renewable, and carbon-neutral energy solution. Despite the challenges that lie ahead, continued research and development in this field hold the key to a sustainable future, free from reliance on non-renewable fossil fuels.。
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Enhancing optimal feeder assignment of the multi-headsurface mounting machine using genetic algorithmsShaoyuan Li*,Chaofang Hu,Fuhou TianInstitute of Automation,Shanghai Jiao Tong University,800Dongchuan Road,Shanghai200240,ChinaReceived28November2005;received in revised form26January2007;accepted1February2007Available online4April2007AbstractThe problem of minimizing the time required to populate a printed circuit board using a multi-head surface mounting machine is considered in this paper.The multi-head surface mounting machine is becoming increasingly popular due to its merit of picking or placing multiple components simultaneously in one pick-and-place operation,which reduces much portion of the assembly time.The complexity of the optimization problem of minimizing the assembly time results in that acquiring its desired solution is difficult.The total assembly time depends on two optimization problems:feeder assignment problem and pick-and-place sequencing problem.Although these two problems are interrelated,they are solved, respectively.Feeder assignment problem is one crucial problem of affecting surface mounting machine’s productivity directly.Optimal feeder assignment can decrease sum of time of moving along slots,moving from slot to PCB and moving from PCB to slot for placement heads griping components after predetermining pick-and-place sequence.For it is of a combinatorial nature and NP-hard,there is no exact algorithm for it.As an efficient and useful procedure for solving the combinatorial optimization problems,the genetic algorithm with specific crossover and mutation operators is proposed in this paper.The running results show that the proposed method performs better than the conventional methods.#2007Elsevier B.V.All rights reserved.Keywords:Printed circuit board(PCB);Multi-head surface mounting machine;Feeder assignment problem;Genetic algorithms1.IntroductionAutomated manufacturing in the electronics industry is becoming more popular as product varieties increase,product volumes decrease and product life cycles shorten.Printed circuit boards(PCB)are the brains’of electronic products,and their cost plays a major role in determining the competitiveness of an electronicsfirm.With the recent development of the component manufacturing technology,surface mount technol-ogy(SMT)has replaced pin-through-hole technology.Because SMT permits a large number of components to be mounted on a single PCB,assembly time is the most significant factor in PCB production cost.The problem of minimizing the PCB assembly time with surface mounting machine is considered in this paper.For surface mounting machine,the total PCB assembly time depends on two optimization problems:feeder assignment problem and pick-and-place sequencing problem.Although these two problems are interrelated,they are solved,respectively.As suggested in most papers,the feeder assign-ment problem is treated as a Quadratic Assignment Problem (QAP)[1,2]and Minimum Weight Matching Problem (MWMP)[3],the pick-and-place sequencing problem can often be modeled as a Traveling Salesman Problem(TSP)[2–5].Being the combinatorial nature and NP-hard of these two problems,the best solution is not acquired so that they can usually be solved by many heuristic algorithms[6,7].In addition,other methods such as tabu[8],simulated annealing [9]and so on,are also presented in the literatures.In view of their characteristic such as near optimization,high speed and easy realization,genetic algorithms(GAs)as a search strategy have received much attention and been successfully applied in the combinatorial optimizationfields [10–12],especially production environment problems[13–16] such as FMS[17]and VLSI[18],and location problem[19] over the last decade.Moreover,GAs were also used as tool for PCB assembly optimization.Lee et al.focused on the systematic GA for a multi-head surface mounting machine [20].The assignment of feeders to heads and construction of feeder-groups were,respectively,optimized by their GA. Finally,the whole multi-head problem was identical to the/locate/asocApplied Soft Computing8(2008)522–529*Corresponding author.E-mail address:syli@(S.Li).1568-4946/$–see front matter#2007Elsevier B.V.All rights reserved.doi:10.1016/j.asoc.2007.02.012single-head problem.Khoo and Loh designed a prototype GA enhanced planning system for surface mounting machine with a rotary turret,a moving feeder carriage and a moving board[21]. In addition,some other researchers studied the productivity of SMT assembly line.Dikos et al.concentrated their research on optimizing the portion of the SMT manufacturing process with the similar machine to that of Khoo and Loh in high-mix environments[22].The GA was employed tofind the near optimal assignment of the feeder.Further,they extended the algorithm to the SMT with multi-station placement machines [23].Multiple setup PCB assembly planning using GA was considered by Deo et al.[24].Which setup each component is assigned to is added to the original optimization problems for surface mounting machine.In this paper,the feeder assignment problem is studied.For the advantages of GAs over the other conventional methodssuch as towards the global optimization instead of local minima,this paper proposes a GA to solve this problem for the SONY SE-1000surface mounting machine after the pick-and-place sequencing problem has been taken as TSP and solved by Nearest Neighbor.The GA presented here is used with specific integer coding,uniform order crossover and exchanging mutation operators.The rest of this paper is organized as follows.In Section2, the structure and principle of this multi-head surface mounting machine is described.Assumptions,and mathematical descrip-tion of PCB assembly time for feeder assignment problem are given,too.In Section3,the implementation details of the proposed GA is provided.The efficiency of the optimization strategy based on GA developed in this paper is verified through experiments in comparison with the conventional methods in wide industry use in Section4.Section5concludes this paper.2.Problem descriptions2.1.Machine introductionThe multi-head surface mounting machine is becoming increasingly popular due to its strong point that it can mount more components than the single head machine simultaneously. The machine concerned in this paper is SONY SE-1000surface mounting machine as in Fig.1.It uses a placement mechanism mounted on a rotating turret,with multiple heads,that can pick-and-place more components every time.The introduction of this machine are described in the following:Feeders:Arefixed and lie in the slots of the both sides of the machine,which have40slots on every side.Each feeder that only owns the same components must be assigned to exactly one slot.Rotating turret:Has12heads which can move in X,Y and Z simultaneously.Heads and nozzles:Are located on the rotating turret.Each head has a nozzle that grips and holds a component until it is placed on the PCB.Nozzles of different diameters are used depending on the size of the component to be retrieved.Thenozzles cannot be automatically changed during assembly.Therefore,they can only be altered at the beginning of the production.PCB:Cannot move during assembly.Before production,the feeders must be assigned to the corresponding slots according to the optimization results of the programmed optimization software,and the required nozzles should also be installed.After preparation,the PCB is transferred into the machine by a conveyor belt andfixed at a predetermined position for assembly.In one mounting cycle, the rotating turret moves along the slots,and picks as many components as possible.Then it moves back to the corresponding position of PCB and mount these components.2.2.Assumptions and statementAssumptions are given in terms of practices in real setting and operation of SONY SE-1000surface mounting machine.(1)The turret will rotate a certain angle after picking or placingone component.It moves and rotates concurrently except for gripping more same type of components from one slot.And the speed of rotating is much higher than that of moving.Therefore,only the moving time is considered during assembly.Since the mounting sequence is given,the rotating time for the picking more components from the same feeder can be regarded as constant and ignored during computation.The routine of the turret along the slots in every cycle is identical from right to left in Fig.1. (2)As for every component is concerned,the action of pickingor placing is always same and short.Therefore,the total action time is constant in any assembly sequence so that it can be neglected when computing assembly time.(3)In real production,the moving patterns are differentaccording to the type of the components.For example,if the quality of the component is different,the speeds of picking and placing are different,too.When the turret moves from one position to another,the alternation of its speed is complex,including accelerating,uniform and decreasing.These factors make the accurate computation difficult.So Fig.1.SONY SE-1000surface mounting machine.S.Li et al./Applied Soft Computing8(2008)522–529523the average speed is used in this paper for convenience.The moving speed is considered as constant.Then the assembly time is proportional to the moving distance of the turret.(4)Although the feeders can be assigned to the both sides of themachine,the concern in this paper is usually about assembly on one side.The total type number of the components is limited less than or equal to40.Based on the assumptions of the real assembly above,the following notations are used in the development:n:The number of the components to be mounted.m:The number of the component types,it satisfies 1m40for SONY SE-1000surface mounting machine.i,j:The order number of the components to be mounted, with1i,j n.s,s0:The order number of the slots,with1s,s040.t(i):The assembly cycle that the component i mounted belongs to.s l tðiÞ;s rtðiÞ:The farthest left and right slot in the same cycle t(i).d i(i+1):The assembly distance computed including pickingand placing between the component i and i+1.d pp(i,j):The Tchebycheff distance between the component iand j on PCB.d pf(i,s):The Tchebycheff distance between the component ion PCB and the slot s.d ff(s,s0):The Tchebycheff distance between the slot s and s0.d fp(s,j):The Tchebycheff distance between the slot s and thecomponent j on PCB.Then,the optimization objective of the whole assembly problem is formulated as follows:minX nÀ1i¼1d iðiþ1Þ(1) whereThe objective function in(1)describes the travel distance of the turret during assembly.It consists of four terms expressed in the constraints(2),i.e.the distance between the components on the PCB,the distance along the slots,the distance from slot to PCB and the distance from PCB to slot during one cycle.It must satisfy the system constraint that only one feeder can be assigned to one slot.Feeder assignment problem and pick-and-place sequencing problem determine the total assembly time. Feeder assignment problem is one crucial problem of affecting surface mounting machine’s productivity directly.The better feeder assignment problem is solved,the less sum of time of moving along slots,moving from slot to PCB and moving from PCB to slot for placement heads griping components is obtained.This paper focuses on the feeder assignment problem after predetermining pick-and-place sequence.The assumption that the pick-and-place sequence is given beforehand means the term d pp(i,i+1)in(2)is constant and can be ignored when t(i)=t(i+1).So the optimization objective of feeder assign-ment problem is reformulated in the following expression: minX nÀ1i¼1f d pfði;s r tðiþ1ÞÞþd ffðs r tðiþ1Þ;s l tðiþ1ÞÞþd fpðs l tðiþ1Þ;iþ1Þg(3) 3.Optimal solution by genetic algorithmsThis section will provide an overview and application of GAs,including the real genetic operators.3.1.OverviewSince this optimization problem is NP-hard and combina-torial nature,the traditional optimization techniques are unsuitable.According to their extensive applications in assembly sequence planningfield[25–27],GAs,which are a sort of population-oriented stochastic search techniques and adaptive methods to solve optimization problems,can be used to acquire the satisfactory solution of feeder assignment problem.Based on the mechanics of genetics and of nature selection,GAs differ from other optimization methods and search procedures.GAs are mainly composed of three basic operators: reproduction,crossover mutation.When solving a problem using GAs,first a proper representation andfitness measurement must be designed.Many representations are possible,and will work.Some are better than the others. Devising the termination criterion should be the next step. The termination criterion usually allows at most some predefined number of iterations and verifies whether an acceptable solution has been found.New population will becreated by genetic operators without satisfying the criterion, and iteration will continue.3.2.CodingTraditionally,chromosomes are simple binary vector.The simple representation is not convenient for the complex feeder assignment problem.A better method is needed to change the chromosome syntax tofit the problem.Referring to the coding examples of representing search nodes for multiprocessor scheduling in reference[28],expressing assembly sequence of product structure in reference[26]and allocating rectangular pieces onto a strip in reference[29],a special coding is used to represent the chromosome instead of the conventional0–1d iðiþ1Þ¼d ppði;iþ1ÞtðiÞ¼tðiþ1Þd pfði;s r tðiþ1ÞÞþd ffðs r tðiþ1Þ;s l tðiþ1ÞÞþd fpðs l tðiþ1Þ;iþ1ÞtðiÞ<tðiþ1Þ(2)S.Li et al./Applied Soft Computing8(2008)522–529524coding in this paper.Each gene of the chromosome denotes a slot. This representation is similar to those in references[20–24].For references[20,21,24],the chromosome is made up of two parts: one represents the feeder assignment and the other represents the pick-and-place sequence.In this paper,however,only the former problem is solved such that the genes of the chromosome just denote the feeder assignment.As for SONY SE-1000surface mounting machine with40slots presented in this paper,the chromosome includes40genes.With the notations in Section2, some definitions are given.Let V and W are integer sets,i.e. V={1,2,...,40}and W={mÀ40,mÀ39,...À1,1,...,m}. Let the chromosome is defined as¯g¼½gð1Þ;gð2Þ;...;gð40Þ , where g is a function representing the mapping g:V!W,and g(s)¼g(s0)if s¼s0.The integer number in set W corresponding to every gene in the chromosome stands for whether one slot own one type of components or not.For the position s of the chromosome,g(s)>0expresses that the type g(s)of components have been assigned to the corresponding slot s.Inversely, g(s)<0describes that there is not components in the slot s.The negative integer numbers from big to small are the deputies of the empty slots from left to right in the chromosome.This coding method fully satisfies the system constraint in Section2.For example,there is a PCB including11types of components shown in Table1.The component type3is assigned to slot5with g(5)=3and the component7in slot11 with g(11)=7,and so on.Because there is no special large component occupying more slots and requiring empty slots in this paper,this chromosome coding is different from that of [22,23].3.3.Creation of initial populationThe individual number of the population is100.Variable a is the iterative number of individuals in initial population with a100and b is the iterative number of component types with b m.The procedure creating the initial population is as follows:Step1:a=1.Step2:b=1.Step3:A slot number s which is greater than0and less than or equal to40is randomly generated.If there is none in the gene s of the chromosome a,the value of this gene,i.e.g(s), is considered as b and go to step4.It means that if there is no component in the slot s,the component type b can be assigned to the slot.Otherwise repeat step3.Step4:b=b+1,if b>m,the negative integer numbers in set W fromÀ1are degressively assigned to the empty positions of the chromosome from left to right,then repeat step4till each element of¯g has definite value,and go to step5.Otherwise go back to step3.Step5:a=a+1,if a>100,the initial population has been created,and program stops.Otherwise go back to step2.During the above steps,every component type is randomlyassigned among the40slots,which means that the initial population is created stochastically.However,the random search maybe increase the generations of evolution and computation in spite of satisfactory results.So some better chromosomes can be selected as the individuals of the initial population.This paper predetermines a reasonable range of slots according to the size of the PCB and the number of the component types.For example, exclude the slots far away from the PCB,and require the components chosen in the reasonable range.But it may be result in the premature despite the good initial individuals.3.4.Fitness functionThe paper’s purpose is to get the satisfactory solution of feeder assignment problem,which minimizes the production time as much as possible.Thefitness function can be acquired based on the objective function(3).Since GAs could have better chances for the surviving individuals with relatively higherfitness but the aim of(3)is minimum,the paper obtains thefitness function by transforming(3).Thefitness function is expressed byfitness.Letfitness¼MÀv(4) where v is the objective function(3),i.e.v¼X nÀ1i¼1f d pfði;s r tðiþ1ÞÞþd ffðs r tðiþ1Þ;s l tðiþ1ÞÞþd fpðs l tðiþ1Þ;iþ1Þg(5) and M is the biggest objective value of(5)among all indivi-duals.From the chromosome coding¯g,the positions of com-ponents assigned in each assembly cycle are got by decoding sothat the coordinates of variables s ltðiÞ,s rtðiÞcan be known.The distances along slots,between the slots and components on PCB can be computed,too.3.5.ReproductionThe selection tactics is roulette wheel selection in this paper. Different from the methods in references[20–24],itsfitness function must be modified when the individual with the biggest objective function value is not selected based on(4).If the fitness function value of individual a is equal to0,and only less than that of individual a0,then assumefitnessðaÞ¼12fitnessða0Þ(6)Table1Chromosome codingSlot(s)(1)...(5)...(11)(12)(13)(14)...(18)(19)(20)(26)(27)(28)...(32)(33)(34)(35)(36) (40)g(s)À131À10924À14À1511À21810À25576À29S.Li et al./Applied Soft Computing8(2008)522–529525so that every individual will probably be selected.In this paper,about 15%of all individuals will be replaced during reproduc-tion.3.6.CrossoverThere are many new types of crossover operators [30,31]to be adopted in order to get the better solution of assembly planning problem.In this paper,uniform order crossover with less iteration developed by Davis [32]is required to satisfactory solution for the feeder assignment problem as [22,23].Firstly,the selection strategy of the parents is roulette wheel selection in order to make the individual with high fitness evolve.The crossover probability is 50%in this paper.Secondly,for simplicity the chromosome with nine genes expressed by integer is considered as an example according to the coding of feeder assignment problem including four types of components in nine slots.Uniform order crossover is shown in Fig.2.The procedure of uniform order crossover above is as follows:Step 1:A binary string having the same number of bits with the above individuals is randomly generated.Step 2:The genes in the positions of parent A,in which the bits are equal to 1in binary string,are copied to the same position of offspring A,so do parent B and offspring B.Step 3:Check up every gene of parent B orderly,and insert the genes not existing in offspring A into its empty positions from left to right,the same to parent A and offspring B.The crossover stops.3.7.MutationThe individuals are randomly selected with mutation probability 5%.Because of the special coding in this paper,the result of chromosomes will contain two copies of the same integer,and thus result in an infeasible solution if simple mutation operator is used.Therefore,the mutationoperator used here is exchanging mutation.An example of the chromosome with nine genes is taken as the same as that in Section 3.6.The exchanging mutation is shown in Fig.3.The procedure of exchanging mutation above is as follows:Step 1:Two genes are stochastically chosen in the same chromosome.Step 2:These two genes are exchanged in this chromosome.4.Software and experiments 4.1.Software introductionThe optimization software is programmed by Visual C++6.0language in light of the requirement of SONY SE-1000surface mounting machine.The data of PCB including the number of components,the number of component types,position of each component,the number of nozzles required,are regarded as inputs.Its outputs are the feeder assignment result and the final assembly time that can be acquired after running the optimization software.The demo of this software shows the assembly structure and assembly procedure using the assign-ment result as in Fig.4.Referring to Fig.4,the functions of the software are expressed as follows:Button ‘‘configuration’’:Inputs the component data of PCB and sets optimization parameters.Button ‘‘sequence’’:Begins the optimization procedure and obtain the satisfactory result of feeder assignment problem.Button ‘‘play’’:Is pressed down,and the assembly simulation procedure will be shown.Blocks on the up and down sides :Express the slots,the red ones express the slots owning feeders,the blue ones are the empty slots.Dots :Denote all the components on PCB,the red and white ones stand for those mounted and unmounted components,respectively.Little window ‘‘nozzles illustration’’:Is located on the left and up corner,shows the pick-and-place sequence of placement heads.Illustrations such as picking or placing time of the component being mounted,total assembly time,current assembly action and type of component being gripped,can be seen on it.The specific pick-and-place action can be shown through pressing down the buttons on it step bystep.Fig.2.Uniform ordercrossover.Fig.3.Exchanging mutation.S.Li et al./Applied Soft Computing 8(2008)522–5295264.2.ExperimentsIn this software,the optimization method is treated as a module so that various optimization strategies can be studied and compared.To evaluate the optimization method for feeder assignment with GA proposed in this paper,the total assembly time is compared with that of the typical industry tactic such as a greedy assignment algorithm,which is commonly used to solve the problem of assigning feeders under the condition of the same pick-and-place sequence acquired by Nearest Neighbor TSP algorithm.The pick-and-place time is con-sidered as given constant as in Section2.Then the total assembly time being computed is the sum of(3)and this constant.In GA presented here,the number of generation is 1000,the size of population is100,the number of each chromosome’s genes is40and the crossover probability and mutation probability are,respectively,50%and5%.The GA module is programmed through Visual C++6.0according to the proposed algorithm with uniform order crossover and exchan-ging mutation.The data of six different PCBs from real production are given in Table2,which includes the number of components, that of component types and of nozzles types required.For example,the data of a little PCB with a few components among these six PCBs are listed in Table3.Table4shows the optimization results of these PCBs using the two methods.For each board,the second column gives the result of the traditional industry algorithm.The results of GA are expressed from the third tofifth column,i.e.the average initial results,average satisfactory results and the most satisfactory result for running10times.The sixth and seventh columns give comparison results between the two algorithms. The comparisons of average saved cost of six PCBs are given in thefinal row.The optimization results in Table4indicate that all over the range of tested boards in Table2,the method proposed in this paper,respectively,results in10.4%the best savings,5.0%the worst savings and7.2%the average savings of most satisfaction over the conventional industry method in total assembly time. Moreover,the best savings,the worst savings,and the average savings of average satisfaction are10.4%,3.5%and6.2%,Fig.4.Assembly optimization software.Table2Data of6PCBsPCB DataNumber of components Number ofcomponent typesNumber of nozzletypes requiredPCB138122PCB2238322PCB3301253PCB4448393PCB51562PCB694173S.Li et al./Applied Soft Computing8(2008)522–529527respectively.Therefore,the optimization method with GA presented in this paper is superior to the conventional industry strategy.5.ConclusionIn this paper,the optimization for the feeder assignment problem by a GA with specific genetic operators is presented,given that the order of pick-and-place is known.The genetic model is formulated,and the satisfactory solution is obtained.The above experimental results prove the optimization method with GA is efficient.The optimization software runs in computer with Pentium III 450MHz CPU,which costs less than 1min for the data of any PCB of the experiments.This indicates that the proposed GA is significant to increase the productivity in real production for a number of different PCBs.This GA can be improved further.Some parameters,e.g.the number of population,the crossover probability and the mutation probability can be changed according to the convergence.In addition to the optimization for feeder assignment problem,the pick-and-place sequencing problem is also optimized after assigning the feeders.Then better results can be acquired by iteration.AcknowledgementsThis work was supported by the National Natural Science Foundation of China under Grant 60474051,the Specialized Research Fund for the Doctoral Program of Higher Education of China under Grant 20060248001and partly by the program for New Century Excellent Talents in University of China (NCET).The authors are grateful to the anonymous reviewers for their helpful comments and constructive suggestions with regard to this paper.References[1]T.Leipa¨la ¨,O.Nevalainen,Optimization of the movements of component placement machines,Eur.J.Operational Res.88(1989)167–177.[2]I.Or,E.Demirkol,Optimization issues in automated production of printedcircuit boards:operations sequencing and feeder configuration problems,in:Proceedings of 1995INRIA/IEEE Symposium on Emerging Technol-ogies and Factory Automation,1995,pp.479–487.[3]S.H.Lee,B.H.Lee,T.H.Park,A hierarchical method to improve theproductivity of a multi-head surface mounting machine,in:Proceedings of the 1999IEEE International Conference on Robotics and Automation,1999,pp.2110–2115.[4]R.Kumar,H.Li,Integer programming approach to printed circuit boardassembly time optimization,IEEE ponents Packaging Manuf.Technol.Part B 18(4)(1995)720–727.Table 3Example of PCB’data Component typeData X -coordinateY -coordinate Component code Nozzle type 10K-0603À1.213R11No.410K-0603À18.15 5.3R21No.410m F/16V À15.812.35C11No.510m F/6.3V À1.26C12No.510m F/6.3V À1810C22No.52N4401À4 3.35Q11No.52N4401À8 3.35Q12No.52N4401À14.813.8Q21No.52N4401À11.1514.8Q22No.5510V -0603À6.913.2R13No.4510V -0603À11.1510.2R23No.462V -0603À4.27.3R12No.462V -0603À4.212.05R15No.462V -0603À9.457.35R22No.462V -0603À9.454.5R25No.4Table 4Comparison between optimization method using GA and conventional industry algorithm PCBConventional industry algorithmGASaved cost (%)Average initial solution Average satisfactory solution Most satisfactory solution Average satisfaction Mostsatisfaction PCB 1694.53940.76622.53622.5310.410.4PCB 22168.613281.962062.612060.62 4.9 5.0PCB 35662.487691.385333.255300.70 5.8 6.4PCB 417107.2622620.8216218.3215925.88 5.2 6.9PCB 515388.3322243.6514848.5114539.95 3.5 5.5PCB 634611.5140436.7332035.7831565.037.48.8Average save cost of six PCBs6.27.2S.Li et al./Applied Soft Computing 8(2008)522–529528。