bs1881-128
钢筋保护层厚度测试仪操作说明书
PROFOMETER5钢筋直径/保护层厚度测试仪操作说明书欧亚星宇科技地址：丰台区南三环中路70号南曦大厦A座605室邮编：100075：0/1/2/3 传真：2/3.yiqi518.目录1概述 (2)1.1基本信息1.2用途1.3安全规则1.4符合的标准2产品描述 (2)2.1S型2.2Scanlog型2.3多功能探头2.4扫描路径探头3开始 (4)3.1仪器连接3.2启动显示器4设置 (4)4.1钢筋直径4.2编号4.3保护层下限值4.4邻近钢筋的修正4.5语言4.6基本设置4.7数据输出4.8数字检测方式4.9钢筋扫描方式4.10灰度方格显示方式5检测步骤 (5)5.1用数字检测方式检测5.2检测不足的保护层厚度5.3检测钢筋直径5.4用CyberScan使钢筋可视化5.5用灰度方格显示方式检测6技术参数 (11)1. 概述1.1基本信息本仪器采用一流的技术，操作之前请仔细阅读本操作说明书。
1.2用途 本仪器用于无损地定位钢筋、检测保护层厚度和钢筋直径。
1.3安全规则 本仪器应由熟悉本仪器操作的人操作。
1.4符合的标准SIA 162/DIN 1045/DGZfP B2/BS 1881:Part 2042．产品描述2.1 S 型（基本型）Profometer5是一种轻便、精巧的仪器，能无损地检测钢筋的位置、保护层厚度和钢筋直径。
检测方法基于涡流和脉冲原理。
图2.1 S 型2.2SCANLOG 型图2.2 Scanlog 型以上两种型号，都能直接连接打印机打印数据或将数据传输到电脑。
2.3多功能探头2.3.1功能多功能探头的使用是有方向性的，当它与钢筋轴向平行时最灵敏，当与钢筋轴向垂直时最不灵敏。
因此，使用时探头方向应该和钢筋轴向平行。
多功能探头有小围和大围之分。
使用 箭头可以在两种检测围之间切换。
激活的检测围显示在显示屏上。
当保护层大于图中所示的围时，才采用大检测围。
例如，当钢筋直径为16mm ，保护层为60mm 时，应采用大检测围。
42-97-M 澳门混凝土标准
水化學氧份需求 重鉻酸 氧需求 標准測試方法
ASTM D 1293
水 pH 值標準測試方法
ASTM D 1426
水阿摩尼亞氮含量標準測試方法
ASTM D 1888
水溶解及不溶解物標準測試方法
RILEM CPC7
直接拉力試驗 最後建議 1975
BS 812:Parte 110
骨料壓碎值測定方法 ACV
ASTM C 131
粗骨料坑磨能力測試方法 –LosAngeles 磨耗試驗
ASTM C 136
骨料篩分法
ASTM C 142
骨料含泥量測試方法
ASTM C 170
天然建築用石抗壓強度測試方法
ASTM C 227
水泥及骨料之 反應測試方法 水泥 砂漿柱體法
ASTM C 289
骨料反應測試方法 化學方法
混凝土 水泥砂漿及水泥漿混合劑 測試方法–混凝土泌水性測定
混凝土 水泥砂漿及水泥漿混合劑 測試方法–毛細管吸水性測定
混凝土 水泥砂漿及水泥漿混合劑 測試方法–液體混合劑之密度測定
混凝土 水泥砂漿及水泥漿混合劑︰ 測試方法–固體含量測定
混凝土 水泥砂漿及水泥漿混合劑 測試方法–酸 度測定
混凝土 水泥砂漿及水泥漿混合劑 測試方法–氯化物含量測定
組成材料 1 水泥 可用於生 混凝土之各種水泥均 1 被列於 水泥標準 中 2 骨料 骨料之特性 標準文 及要求值均列於表一中 表一骨料特性
特性
測試標準
要求
抗壓強度或
ASTM C170
>= 50 MPa
粗骨料之力 學 強度 (1)
壓碎強度或
Los Angeles 損耗 (2)
BS 812: Part 110
QPCR及QRT-PCR系列产品
Invitrogen的ICFC系列产品促销1．QPCR及QRT-PCR系列产品Invitrogen公司专门为中国客户提供的定量PCR试剂盒，结合了 UDG 防止残余污染技术和SYBR® Green I 荧光染料（存在于SYBR® Green I荧光定量PCR试剂盒中），在美国接受了严格的质量监控，可提供极高灵敏度的目的序列定量检测，线性剂量低，反应浓度范围很大。
qPCR Supermix-- 即用型反应剂，专为高特异性、实时定量DNA扩增设计UDG-- 防止携带污染物，减少克隆片段假阳性结果ROX参考染料-- 适用ABI仪器的校正染料产品信息活动时间：即日起至2009年4月30日2．Gibco南美胎牛血清即日起凡优惠价¥1780购买Gibco胎牛血清500ml（目录号:C2027050）即可获赠送价值¥250现金抵用券。
您可以凭现金抵用券在英韦创津公司购买任何商品，此券有效期至2009年5月31日。
产品信息活动时间：即日起至2009年4月30日独特的采集方式：GIBCO采用无菌心脏穿刺的方式采血原装直送，避免污染：原产地采集、加工、检测、包装。
完善的质控：采集、处理、检测、运输等环节都有文件和证书。
3．Invitrogen TA Cloning克隆产品专门用于克隆Taq聚合酶扩增的PCR产物。
采用pCR载体，能产生80%以上的重组产物，90%以上重组产物都包含插入片段。
产品信息活动时间：即日起至2009年5月31日附：pCR载体优点及图谱：3’-T突出端可直接连接Taq扩增的PCR产物可选择T7或T7和Sp6启动子进行体外RNA转录和测序侧向EcoRⅠ位点的通用多接头位点方便了插入片段的切离可以选择卡那霉素或氨苄青霉素进行筛选非常简便的蓝/白克隆筛选具有M13正向和反向引物位点，方便测序4．GIBCO液体培养基系列产品创立近50年的历史，品质优秀，产品种类丰富；为了中国用户利益，特建立国内生产线；所有产品，从原材料到生产全部按照GIBCO质量标准进行，每批均送抵美国公司总部质检合格后，才在国内销售。
Canin+钢筋锈蚀仪中文说明书
机械和电机零件和附件：6 个月
仪器时，最多可购买 3 年延长保修（适用于电极指示器。
延长保修服务必须在购买时或购买之日起 90 天内购买。
服务及保修信息
致力于通过我们的全球服务和支持机构，为 CANIN
提供全面的支持。
此外，每一个 CANIN+电极指示器均享受
Proceq 的标准 2 年保修，还可以选择延长保修。
对此信息的完整性和/或精确性不做任何形式的担保，并排除所有责任。
Proceq SA 针对其制造和/或销售的任何产品的
... 50 多年专业测量经验!... 50 多年专业测量经验！
频率曲线累积频率曲线
此外，该仪器可以测量混凝土的电阻率。
低混凝土电阻率表明钢筋腐蚀的可能性更大，锈蚀率也更大。
混凝土电阻率的变化范围很广，这主要取决于当地的条件和环境的影响。
电阻率和电位测量的相
· 混凝土电阻率可以通过四点 Wenner 探头来测量。
碧云天生物技术 3M NaAc pH5.2 (Sterile, DNase free)说明书
碧云天生物技术/Beyotime Biotechnology订货热线：400-168-3301或800-8283301订货e-mail：******************技术咨询：*****************碧云天网站微信公众号网址：3M NaAc, pH5.2 (Sterile, DNase free)产品编号产品名称包装ST351 3M NaAc, pH5.2 (Sterile, DNase free) 100ml产品简介：3M NaAc pH5.2 (Sterile, DNase free)，即无菌，无DNA酶污染的醋酸钠缓冲液，是一种常用的分子生物学试剂。
本产品无菌，无DNA酶污染，主要用于DNA的乙醇沉淀等。
NaAc即醋酸钠(Sodium Acetate)，也称乙酸钠，分子式为CH3COONa，分子量82.03，CAS号127-09-3。
DNA是一种多聚阴离子水溶性化合物，在DNA提取过程中，经常需要加入醋酸钠等适当的缓冲液并用乙醇等适当有机溶剂来沉淀DNA。
其原理是乙醇能夺取DNA周围的水分子，使DNA失水从而易于聚合；同时乙醇也能够消除DNA的水化层使带负电荷的磷酸基团暴露出来与醋酸钠缓冲液中的高浓度钠离子(Na+)结合，从而减少DNA分子之间的同性电荷排斥力而更易于聚合，最终形成DNA钠盐沉淀。
在低温环境下，这种促沉淀效应会被加强，因此对于低浓度DNA或者较难沉淀的小分子量DNA通常宜在-20ºC或-70ºC等低温条件下沉淀，以提高沉淀效率。
本产品常用于各种常见DNA样品的浓缩和纯化。
包装清单：产品编号产品名称包装ST351 3M NaAc, pH5.2 (Sterile, DNase free) 100ml—说明书1份保存条件：室温保存。
注意事项：本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
碧云天pCMV-jGCaMP7c高对比度钙离子荧光探针说明书
碧云天生物技术/Beyotime Biotechnology 订货热线：400-1683301或800-8283301 订货e-mail ：******************技术咨询：*****************网址：碧云天网站 微信公众号pCMV-jGCaMP7c (高对比度钙离子荧光探针)产品编号 产品名称包装 D2863-1µg pCMV-jGCaMP7c (高对比度钙离子荧光探针) 1µg D2863-100µgpCMV-jGCaMP7c (高对比度钙离子荧光探针)100µg产品简介：pCMV-jGCaMP7系列质粒(jGCaMP7b/jGCaMP7c/jGCaMP7f/jGCaMP7s)是用于钙离子(Ca 2+)荧光检测的哺乳动物细胞表达质粒，表达的是经过突变和优化并适用于细胞或组织中钙离子荧光检测的M13-GFP-CaM 融合蛋白。
M13-GFP-CaM 融合蛋白这个钙离子探针(calcium probe)中，环状排列的绿色荧光蛋白(circularly permuted green fluorescent protein, cpGFP)一端和钙调蛋白(calmodulin, CaM)融合表达，另一端与可以和钙调蛋白CaM 相互作用的肌球蛋白轻链激酶M13 (也称RS20)肽链融合表达。
这样当钙离子结合钙调蛋白时，可以改变钙调蛋白的构象，同时也会影响和钙调蛋白相互作用的M13的构象，钙调蛋白和M13的构象改变最终导致GFP 的构象改变，从而影响其荧光的强弱，最终实现对于钙离子浓度变化的检测。
Tag M13 Linker1 cpGFP Linker2 CaM图1. GCaMP7系列蛋白结构图。
其中pRSET A Tag 中含有6X His tag 。
碧云天构建的pCMV-jGCaMP7系列质粒(jGCaMP7b/jGCaMP7c/jGCaMP7f/jGCaMP7s)是最新一代基因编码的钙离子探针系列，适用于常见细胞的细胞内钙离子水平变化检测，并且特别适合用于监测特定条件下神经元中的钙离子水平的变化。
SIGMA-ALDRICH MES hydrate M8250说明书
SIGMA-ALDRICH SAFETY DATA SHEETVersion 4.8Revision Date 08/02/2016Print Date 11/14/2016 1. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifiersProduct name : MES hydrateProduct Number : M8250Brand : SigmaCAS-No. : 1266615-59-11.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses : Laboratory chemicals, Synthesis of substances1.3 Details of the supplier of the safety data sheetCompany : Sigma-Aldrich3050 Spruce StreetSAINT LOUIS MO 63103USATelephone : +1 800-325-5832Fax : +1 800-325-50521.4 Emergency telephone numberEmergency Phone # : +1-703-527-3887 (CHEMTREC)2. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureNot a hazardous substance or mixture.2.2 GHS Label elements, including precautionary statementsNot a hazardous substance or mixture.2.3 Hazards not otherwise classified (HNOC) or not covered by GHS -none3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms : 4-Morpholineethanesulfonic acid2-(N-Morpholino)ethanesulfonic acidFormula : C6H13NO4S · xH2OMolecular weight : 195.24 g/molCAS-No. : 1266615-59-1No components need to be disclosed according to the applicable regulations.4. FIRST AID MEASURES4.1 Description of first aid measuresIf inhaledIf breathed in, move person into fresh air. If not breathing, give artificial respiration.In case of skin contactWash off with soap and plenty of water.In case of eye contactFlush eyes with water as a precaution.If swallowedNever give anything by mouth to an unconscious person. Rinse mouth with water.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2) and/or in section 11 4.3 Indication of any immediate medical attention and special treatment neededNo data available5. FIREFIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, alcohol-resistant foam, dry chemical or carbon dioxide.5.2 Special hazards arising from the substance or mixtureNo data available5.3 Advice for firefightersWear self-contained breathing apparatus for firefighting if necessary.5.4 Further informationNo data available6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresAvoid dust formation. Avoid breathing vapours, mist or gas.For personal protection see section 8.6.2 Environmental precautionsDo not let product enter drains.6.3 Methods and materials for containment and cleaning upSweep up and shovel. Keep in suitable, closed containers for disposal.6.4 Reference to other sectionsFor disposal see section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingProvide appropriate exhaust ventilation at places where dust is formed.For precautions see section 2.2.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly closed in a dry and well-ventilated place.Keep in a dry place.7.3 Specific end use(s)Apart from the uses mentioned in section 1.2 no other specific uses are stipulated8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersContains no substances with occupational exposure limit values.8.2 Exposure controlsAppropriate engineering controlsGeneral industrial hygiene practice.Personal protective equipmentEye/face protectionUse equipment for eye protection tested and approved under appropriate government standards such asNIOSH (US) or EN 166(EU).Skin protectionHandle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (withouttouching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves afteruse in accordance with applicable laws and good laboratory practices. Wash and dry hands.Full contactMaterial: Nitrile rubberMinimum layer thickness: 0.11 mmBreak through time: 480 minMaterial tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)Splash contactMaterial: Nitrile rubberMinimum layer thickness: 0.11 mmBreak through time: 480 minMaterial tested:Dermatril® (KCL 740 / Aldrich Z677272, Size M)datasource:KCLGmbH,D-36124Eichenzell,phone+49(0)665987300,******************,testmethod:EN374If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact thesupplier of the CE approved gloves. This recommendation is advisory only and must be evaluated by anindustrial hygienist and safety officer familiar with the specific situation of anticipated use by our customers. Itshould not be construed as offering an approval for any specific use scenario.Body ProtectionChoose body protection in relation to its type, to the concentration and amount of dangerous substances, andto the specific work-place., The type of protective equipment must be selected according to the concentrationand amount of the dangerous substance at the specific workplace.Respiratory protectionRespiratory protection is not required. Where protection from nuisance levels of dusts are desired, use typeN95 (US) or type P1 (EN 143) dust masks. Use respirators and components tested and approved underappropriate government standards such as NIOSH (US) or CEN (EU).Control of environmental exposureDo not let product enter drains.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesa) Appearance Form: powderColour: whiteb) Odour No data availablec) Odour Threshold No data availabled) pH No data availableNo data availablee) Melting point/freezingpointf) Initial boiling point andNo data availableboiling rangeg) Flash point No data availableh) Evaporation rate No data availablei) Flammability (solid, gas) No data availableNo data availablej) Upper/lowerflammability orexplosive limitsk) Vapour pressure No data availablel) Vapour density No data availablem) Relative density No data availablen) Water solubility No data availableNo data availableo) Partition coefficient: n-octanol/waterp) Auto-ignitionNo data availabletemperatureNo data availableq) Decompositiontemperaturer) Viscosity No data availables) Explosive properties No data availablet) Oxidizing properties No data available9.2 Other safety informationNo data available10. STABILITY AND REACTIVITY10.1 ReactivityNo data available10.2Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available10.4 Conditions to avoidNo data available10.5 Incompatible materialsStrong oxidizing agents10.6 Hazardous decomposition productsHazardous decomposition products formed under fire conditions. - Carbon oxides, Nitrogen oxides (NOx), Sulphur oxidesOther decomposition products - No data availableIn the event of fire: see section 511. TOXICOLOGICAL INFORMATION11.1Information on toxicological effectsAcute toxicityNo data availableInhalation: No data availableDermal: No data availableNo data availableSkin corrosion/irritationNo data availableSerious eye damage/eye irritationNo data availableRespiratory or skin sensitisationNo data availableGerm cell mutagenicityNo data availableCarcinogenicityIARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by ACGIH.NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP.OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA.Reproductive toxicityNo data availableNo data availableSpecific target organ toxicity - single exposureNo data availableSpecific target organ toxicity - repeated exposureNo data availableAspiration hazardNo data availableAdditional InformationRTECS: Not availableTo the best of our knowledge, the chemical, physical, and toxicological properties have not been thoroughly investigated.12. ECOLOGICAL INFORMATION12.1ToxicityNo data available12.2Persistence and degradabilityNo data available12.3Bioaccumulative potentialNo data available12.4 Mobility in soilNo data available12.5Results of PBT and vPvB assessmentPBT/vPvB assessment not available as chemical safety assessment not required/not conducted12.6Other adverse effectsNo data available13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductOffer surplus and non-recyclable solutions to a licensed disposal company.Contaminated packagingDispose of as unused product.14. TRANSPORT INFORMATIONDOT (US)Not dangerous goodsIMDGNot dangerous goodsIATANot dangerous goods15. REGULATORY INFORMATIONSARA 302 ComponentsNo chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 ComponentsThis material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 HazardsNo SARA HazardsMassachusetts Right To Know ComponentsNo components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components2-(N-Morpholino)ethanesulfonic acid hydrate CAS-No.1266615-59-1Revision DateNew Jersey Right To Know Components2-(N-Morpholino)ethanesulfonic acid hydrate CAS-No.1266615-59-1Revision DateCalifornia Prop. 65 ComponentsThis product does not contain any chemicals known to State of California to cause cancer, birth defects, or any other reproductive harm.16. OTHER INFORMATIONHMIS RatingHealth hazard: 0Chronic Health Hazard:Flammability: 0Physical Hazard 0NFPA RatingHealth hazard: 0Fire Hazard: 0Reactivity Hazard: 0Further informationCopyright 2016 Sigma-Aldrich Co. LLC. License granted to make unlimited paper copies for internal use only.The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to theproduct with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. Sigma-Aldrich Corporation and its Affiliates shall not be held liable for any damage resulting from handling or from contact with the above product. See and/or the reverse side of invoice or packing slip for additional terms and conditions of sale.Preparation InformationSigma-Aldrich CorporationProduct Safety – Americas Region1-800-521-8956Version: 4.8Revision Date: 08/02/2016Print Date: 11/14/2016。
BSS_london_BLU产品详细说明
技术参数
输入/输出：提供12路单通道MIC/LINE输入和8路LINE输出 信号处理方式：具有庞大的DSP数字信号处理器功能 DSP数字信号处理器功能：压缩限幅器、增益调整器、相位滤波器、参 数均衡器、延时、噪声门、自动混音器、分频器、矩阵混音器等等 网络通道：提供48路低时延、具有容错功能的数字音频网络通道 频率响应：20Hz～20kHz，（+0.5/- 1dBdB） THD(总谐波失真)：<0.01％（20Hz～20kHz，+10dBu 输出增益） 动态范围：108dB(22Hz～22kHz 无计权) 阻抗：220Ω 连接方式：以太网络为PC连接，RS232接口为第三方连接控制 最大连接长度：设备与以太网设备连接长度可达100m/300ft 控制软件：London Architect 幻像电源供电：+48V 话筒等效输入噪音：150Ω负载，-128dBu 尺寸(H×W×D)：45mm x 483mm x 229mm 重量：3.3kg
Soundweb London的四大优点 易 于 安 装 易 于 设 计 易 于 操 作 易 于 整 合
易于安装
每一台Soundweb London均是一台功能齐全的独立设备， 因此设备可以分散安装在各控制室的功放机柜，而不需要 集中安装在一个地点。通过5类网线，系统设备和以太网 设备便可连接，网线距离建议最长为100米。 最有效的系统设置是通过2条5类网线实现系统的控制、 监视和音频联网。而增加第三条五类网线，即可实现 ConraNet联网的备份传输。 建立联网系统对用户的网络知识的要求并不高，所有的 Soundweb London设备都有自动获取IP地址的功能，无需任 何设置，每一台Soundweb London便可自动取得IP地址。
Uniprise Solutions UNJ10G出口说明书
Uniprise Solutions® Instruction Sheet 860508548 Issue 3, October 2013UNJ10G Outlet InstructionsGeneralThe Uniprise Solutions®UNJ10G outlets support Category 6a applications and are available in a variety of colors. These products are modular RJ45 to insulation displacement connectors (IDC). They are typically installed in faceplates at work locations and provide termination for the horizontal 4-pair cable at the IDC end, and workstation cord insertion at the RJ45 end.The following tools are available to aid in module termination.Material ID Description406477794 D-914 kit − includes impact tool and 110 blade407484971 D-914 impact tool only407728427 Replacement 110 blade for D-914 impact tool760122713 Module removal tool (quantity of 5)860333673 Hand termination tool (hand puck)405423260 KS-22035-L2 spudger toolHow to Contact Us•To find out more about CommScope® products, visit us on the web at /•For technical assistance:- Within the United States, contact your local account representative or technical support at 1-800-344-0223. Outside the United States, contact your local account representative orAuthorized Business Partner.- Within the United States, report any missing/damaged parts or any other issues to CommScope Customer Claims at 1-866-539-2795. Outside the United States, contact yourlocal account representative or Authorized Business Partner.© 2013 CommScope, Inc. All rights reserved For RoHS Inquiries:CommScope Inc.Corke Abbey, BrayCo. Dublin, IrelandAttn: Legal DepartmentPage 1 of 5860508548Instruction SheetTermination ProcedureT568B Wiring ShownPre-Termination Step For Angled EntryNote: Some mounting hardware and box space does not allow for cable entry directly from the rear. If a typical termination from the rear is done and the cable is then bent in the needed direction, performance and reliability can suffer. A proper termination should have the intended entry direction set before seating the conductors.1. Set the intended entry angle before seating theconductors. 2. Pull pairs into the module until the jacket is snugagainst the rear housing. Outer pairs should sweep around the inside pairs and not be pulled into them.Direct Cable EndReverse Cable EndOrange pair1. Each cable end has two pairs routed into the holes and two pairs routed over the top, without crossover orrearrangement. On the Direct cable end, the Orange and Blue pairs enter the holes and the Green and Brown pairs lay over the top. On the Reverse cable end, the Green and Brown pairs enter the holes and the Orange and Blue pairs lay over the top. 2.Pull pairs into the module until the jacket end is snug against the rear housing.Cable positioned at 90prior to seating conductorsoPage 2 of 5 860508548Issue 3, October 20133. To place conductors, hold the pair down on thetermination position and bend it for easy grasp as shown above. Then untwist the pair in a counter-clockwise direction enough to open it up. 4. Push conductors down into slotpositions (check color codes).Maintain pair twist up to theopening for the slots.5. (T568B wiring shown) On the Direct cable end, place Orange and Blue pairs first, then place Green andBrown pairs.Page 3 of 5860508548Instruction Sheetare tightly routed6. (T568B wiring shown) On the Reverse cable end, pull Green and Brown pairs back, eliminate the twist, andpull them tightly into place. Then route Orange and Blue pairs to the front positions.Note: For T568A Wiring -On each cable end, one of the two pairs fed in through the holes is pulled back for the rear positions. Check label color codes.Conductor Seating and CuttingPliersTrim pairsflush1. Use impact tool on HI setting with M110cutting blade to punch conductorsstraight down into slots. When using theimpact tool, the hand puck isrecommended.Or2. Use pliers with wire cap to seatconductors. Use fine edge cutters totrim conductors flush to the modulebody.Page 4 of 51. To remove a module for inspection orrepair, insert prongs of removal tool into openings at sides of module and press firmly.OrThe module wiring cap can also be used to remove a module. Insert prongs of cap into openings at sides of module and press firmly.2. The module can then be removed from theback.Inspection or Repair of TerminationNote: To enable inspection or repair, the wiring cap can be released from the module using the removal tool. A spudger tool can then be used to remove conductors for repair.1.Insert end of removal tool into slot under wiring cap and pivot it forward to release cap.Page 5 of 5。
碧云天 基因组编辑突变检测试剂盒 说明书
碧云天生物技术/Beyotime Biotechnology 订货热线：400-1683301或800-8283301 订货e-mail ：******************技术咨询：*****************网址：碧云天网站 微信公众号基因组编辑突变检测试剂盒产品编号 产品名称包装 D0508S 基因组编辑突变检测试剂盒 25次 D0508M基因组编辑突变检测试剂盒100次产品简介：碧云天生产的基因组编辑突变检测试剂盒(Genome-Editing Mutation Detection Kit)，也称基因编辑突变检测试剂盒(Gene-Editing Mutation Detection Kit)，是一种简单、可靠、快速的基于T7 Endonuclease I (T7EI)的用于检测基因组DNA 在基因编辑后发生突变的试剂盒。
本试剂盒主要利用了T7 Endonuclease I (T7EI)能识别并酶切不完全配对的DNA 双链(也称异源双链DNA ，heteroduplex DNA)的特性。
本试剂盒组分特别齐全。
提供了包括一步法基因组DNA 提取、高保真PCR 扩增、变性退火和T7EI 酶切的全套试剂，并且还提供了阳性对照。
本试剂盒使用特别便捷。
一步法基因组DNA 提取非常快速便捷，并且提取后可以直接用于高保真PCR 扩增，PCR 扩增产物也可以直接用于后续的变性退火和T7EI 酶切。
无需额外的分离纯化步骤。
本试剂盒检测基因组编辑突变的原理如下。
首先需要编辑的细胞、组织或器官通过转染、病毒感染等技术手段表达CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 nucleases)、TALEN (Transcription activator-like effector nucleases)或ZFN (Zinc-finger nucleases)等核酸酶。
牛性IP-10 CXCL10 ELISA试验盒说明书
Bovine IP-10/CXCL10 ELISA KitCatalog Number EB14RB (96 tests), EB14RBX10 (10 x 96 tests)Rev. 7Product descriptionThe Bovine IP-10/CXCL10 ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of bovine IP-10 in cell culture supernatants, plasma, and serum.Contents and storageUpon receipt, store at 2-8°C for 6 months or -20°C for 1 year.Components Cat. No. EB14RB(96 tests)Cat. No. EB14RBX10(10 x 96 tests)Bovine IP-10 Antibody Coated wells, 96-well plate 1 plate10 platesBovine IP-10 Biotin Conjugate 2 vials20 vialsBovine IP-10 Standard, recombinant bovine IP-10 2 vials20 vialsWash Buffer Concentrate (20X)25 mL12 x 25 mLAssay Diluent (5X)15 mL10 x 15 mL Streptavidin-HRP (700X)0.2 mL10 x 0.2 mLTMB Substrate12 mL10 x 12 mLStop Solution8 mL10 x 8 mL Adhesive Plate Covers220Materials required but not suppliedDistilled or deionized waterMicrotiter plater reader with software capable of measuring at 450 nmPlate washer-automated or manual (manifold dispenser)Calibrated adjustable precision pipettes and glass or plastic tubes for diluting solutionsProcedural guidelinesReview the Procedural guidelines and Plate washing directions in the ELISA Technical Guide at for details prior to starting the procedure.Reagents are lot-specific. Do not mix or interchange different reagent lots from various kit lots.Prepare 1X Wash Buffer1.Allow Wash Buffer Concentrate (20X) to reach room temperature and mix to redissolve any precipitated salts.2.Dilute 20 mL of the Wash Buffer Concentrate into 380 mL of deionized or distilled water. Label as 1X Wash Buffer.3.Store the concentrate and 1X Wash Buffer in the refrigerator. Use the diluted buffer within one month.Prepare diluentAssay Diluent should be diluted 5-fold with deionized or distilled water before use.Prepare biotin conjugate1.Briefly spin down the biotin conjugate before use.2.Add 100 µL of 1X Assay Diluent into the vial to prepare a biotin conjugate concentrate.3.Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days).4.The biotin conjugate concentrate should be diluted 80-fold with 1X Assay Diluent and used in step 2 of ELISA procedure.Sample preparation guidelinesCollect samples in pyrogen/endotoxin-free tubes.Freeze samples after collection if samples will not be tested immediately. Avoid multiple freeze-thaw cycles of frozen samples.Thaw completely and mix well (do not vortex) prior to analysis.Avoid the use of hemolyzed or lipemic sera. If large amounts of particulate matter are present in the sample, centrifuge or filter sample prior to analysis.Pre-dilute samples1X Assay Diluent should be used for dilution of serum, plasma, and cell culture supernatant samples.Dilute serum and plasma 2-fold.Because conditions may vary, it is recommended that each investigator determine the optimal dilution to be used for each application.Dilute standardsNote: Use glass or plastic tubes for diluting standards.1.Briefly spin down a vial of lyophilized standard.2.Add 650 µL 1x Assay Diluent (Assay Diluent should be diluted 5-fold with deionized or distilled water before use) into vial toprepare a 30 ng/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Pipette 300 µL 1x Assay Diluent into each tube. Use the 30 ng/mL standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. 1x Assay Diluent serves as the zero standard (0 ng/mL).200200200200200200Diluentvolume300 µL300 µL300 µL300 µL300 µL300 µL300 µLStd1 30 ng/mLStd212 ng/mLStd34.800 ng/mLStd41.920 ng/mLStd50.768 ng/mLStd60.307 ng/mLStd70.123 ng/mLBlank0 ng/mLPrepare 1X Streptavidin-HRP solutionNote: Prepapre the Streptavidin-HRP within 15 minutes of usage.1.Briefly spin the Streptavidin-HRP and pipette up and down to mix gently before use, as precipitates may form during storage.2.Dilute Streptavidin-HRP 700-fold with 1X Assay Diluent.3.Do not store diluted solution for future use.Perform ELISA (Total assay time: 4 hours and 45 minutes)Allow all reagents to reach room temperature before use. Mix all liquid reagents prior to use.IMPORTANT! Perform a standard curve with each assay.Determine the number of 8-well strips required for the assay. Insert the strips in the frames for use. Re-bag any unused strips and frames, and store at 2 to 8°C for future use.1Bind antigen a.For the standard curve, add 100 µL of standards to the appropriate wells (see Dilute standards). Forsamples, add 100 µL of diluted samples (see Dilute samples) to the wells.b.Cover wells and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking.c.Discard the solution and wash 4 times with 1X Wash Buffer. Wash by filling each well with WashBuffer (300 µL) using a multi-channel Pipette or autowasher. Complete removal of liquid at each stepis essential for good performance. After the last wash, remove any remaining Wash Buffer byaspirating or decanting. Invert the plate and blot it against clean paper towels.2Add biotin conjugate a.Add 100 µL of prepared biotin conjugate (see Prepare biotin conjugate) to each well.b.Incubate for 1 hour at room temperature with gentle shaking.c.Discard the solution. Repeat the wash as in step 3.3Add Streptavidin-HRP a.Add 100 µL of prepared Streptavidin-HRP solution (see Prepare Streptavidin-HRP solution) to eachwell.b.Incubate for 45 minutes at room temperature with gentle shaking.c.Discard the solution. Repeat the wash as in step 3.4Add TMB substrate a.Add 100 µL of TMB Substrate to each well. The substrate will begin to turn blue.b.Incubate for 30 minutes at room temperature in the dark with gentle shaking.5Add stop solution Add 50 µL of Stop Solution to each well. Tap the side of the plate gently to mix. The solution in thewell changes from blue to yellow.Read the plate and generate the standard curve1.Read the absorbance at 450 nm. Read the plate within 30minutes after adding the Stop Solution.e curve-fitting software to generate the standard curve. Afour parameter algorithm provides the best standard curve fit.Optimally, the background absorbance may be subtracted from all data points, including standards, unknowns and controls,prior to plotting.3.Read the concentrations for unknown samples and controlfrom the standard curve. Multiple value(s) obtained forsample(s) by the appropriate factor to correct for the sampledilution.Note: Dilute samples producing signals greater than that of thehigest standard in Standard Diluent Buffer and reanalyze.Multiply the concentration by the appropriate dilution factor.Performance characteristicsStandard curve (example)These standard curves are for demonstration only. A standardcurve must be run with each assay.Intra-assay precisionTo determine intra-assay precision, two standard curves and 3 samples for each standard curve are run. The standard curve concentration points as well as the samples are tested in duplicates on a single plate. Two different concentration values are obtained for each sample, using the two separate standard curves. The two concentration values for each sample is compared to each otherusing the CV% calculation. Intra-Assay CV%: <10%Inter-assay precision To evaluate inter-assay precision, the second standard curve is tested on a separate plate along with the second set of samples. Inter-Assay CV%: <12%RecoverySample TypeAverage % Recovery Range (%)Cell Culture Supernatants 11398-119Plasma 10589-120Serum131127-137SpecificityThis ELISA kit shows no cross-reactivity with any of the cytokines tested: Bovine IFN-alpha, IFN-gamma, IL-1alpha, IL-1F5, IL-13, IL-21, MIG, MIP-1beta, TNF-alpha.Linearity of dilutionThe cell culture supernatants, plasma, and serum samples were spiked with recombinant bovine IP-10, serially diluted in sample diluent and evaluated. Observed values were compared to expected values to calculate percent recovery and demonstrate the dilution linearity of the assay.Sample TypeAverage % Expected Range (%)1:2 Dilution1:4 Dilution1:2 Dilution1:4 DilutionCell CultureSupernatants10311998-108115-123 Plasma104123101-109120-126Serum109119100-117109-128SensitivityThe minimum detecable dose of bovine IP-10 is 0.12 ng/mL. Thiswas determined by assaying replicates of zero and the standardcurve. The mean signal of zero + 2 standard deviations read indose from the standard curve is the LLD. This value is thesmallest dose that is not zero with 95% confidence.Limited product warrantyLife Technologies Corporation and/or its affiliate(s) warrant theirproducts as set forth in the Life Technologies' General Terms andConditions of Sale found on Life Technologies' website at/us/en/home/global/terms-andconditions.html. If you have any questions, please contactLife Technologies at /support.Product label explanation of symbols and warningsCatalogNumberBatchCodeTemperaturelimitationUsebyManufacturerConsultinstructions for useCaution, consultaccompanying documentsDISCLAIMERTO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT. Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses.Corporate entity: Life Technologies Corporation | Carlsbad, CA 92008 USA | Toll Free in USA 1 800 955 6288©2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.For support visit /support or contact ************************.22-Nov-21。
北京汇智和源生物技术有限公司细胞与细胞分选产品手册说明书
地址：北京市北京经济技术开发区科创十四街汇龙森18号楼1单元301邮箱：*********************网址：https:// 电话：+86 400-127-6686北京汇智和源生物技术有限公司细胞与细胞分选产品手册FUTURE OF INNOVATIVE REAGENT R&D创新试剂 研发未来公司最早立足于ADMEs 系列产品的开发，助力于药物早期筛选。
经10余年自主研发和产品支持等方面成功经验的积累，公司加大了对药代动力学、药理学、微生物学、免疫学、遗传学和临床医学等领域创新性产品的研发力度，逐步丰富了产品组合。
市售产品经过内部标准或国际标准（例如 OECD 和 ICH）的质量验证，获得了系列资质/专利证书和行业内的广泛认可。
公司核心竞争力是基于我们在化学分析、生物分析、细胞遗传学、基因工程、蛋白质和抗体开发以及免疫分析等领域积累的创新技术能力和经验，我们的使命是为生命科学、医药创新提供产业引领试剂！北京汇智和源生物技术有限公司奉行“创新试剂，研发未来”的发展理念，恪守“诚实、严谨、务实、创新”的企业宗旨，以市场为导向，力求为国内外企业和科研机构提供代表先进技术水平的高品质产品，实现IPHASE 的品牌承诺。
企业理念创新试剂研发未来一家聚焦于生物医药以及生命科学研究领域的高新技术企业完善的知识储备 不懈的科学探索10余年自主研发和产品支持等方面成功经验的积累ABOUT US关于我们北京汇智和源生物技术有限公司是一家聚焦于生物医药以及生命科学研究领域的高新技术企业。
我们的科学团队致力于通过完善的知识储备、不懈的科学探索，竭诚为科学工作者提供高品质的创新生物试剂产品和相关技术服务。
目录前言 (1)原代细胞 (2)免疫细胞亚群 (3)单个核细胞 (4)外周血单个核细胞 (4)脾脏单个核细胞 (5)骨髓单个核细胞 (6)脐带血单个核细胞 (6)动员外周血单个核细胞 (7)红细胞 (7)血小板 (8)原代肝细胞 (9)经代谢验证原代肝细胞 (9)经诱导验证原代肝细胞 (10)经转运体验证原代肝细胞 (10)3D培养原代肝细胞 (11)细胞分离产品 (12)单个核细胞分离试剂盒 (13)红细胞分离试剂盒 (13)血小板分离试剂盒 (13)细胞分选产品 (14)分选试剂盒 (14)抗体 (15)生物素化抗体 (15)流式抗体 (16)磁珠 (17)细胞培养产品 (18)T淋巴细胞活化/增殖 (18)NK细胞培养 (19)原代肝细胞培养 (21)发展历程 (22)重点客户 (23)免疫细胞（Immune cell），由多种不同类型的细胞组成，如单核细胞、巨噬细胞、树突状细胞、自然杀伤细胞等。
BL21-CodonPlus-RP Competent Cells, Part Number 230
BL21-CodonPlus-RP Competent Cells, Part Number 230250*************(24小时)化学品安全技术说明书GHS product identifier 应急咨询电话（带值班时间）::供应商/ 制造商:安捷伦科技贸易（上海）有限公司中国（上海）外高桥自由贸易试验区英伦路412号（邮编:200131)电话号码： 800-820-3278传真号码: 0086 (21) 5048 2818BL21-CodonPlus-RP Competent Cells, Part Number 230250化学品的推荐用途和限制用途BL21-CodonPlus-RP competent cells 230250-41pUC 18 DNA Control Plasmid 200231-42XL10-Gold 2-Mercaptoethanol 200314-43部件号:物质用途:分析试剂。
230250-41BL21-CodonPlus-RP competent cells1 ml（毫升） (10 x 100 µl)200231-42pUC 18 DNA ControlPlasmid0.01 ml（毫升） (0.1 ng/µl)200314-43XL10-Gold2-Mercaptoethanol0.05 ml（毫升）部件号（化学品试剂盒）:230250安全技术说明书根据 GB/ T 16483-2008 和 GB/ T 17519-2013GHS化学品标识:BL21-CodonPlus-RP 感受态细胞，部件号 230250物质或混合物的分类根据 GB13690-2009 和 GB30000-2013紧急情况概述BL21-CodonPlus-RP competent cells液体。
pUC 18 DNA Control Plasmid 液体。
IC集成电路SOP8L框架图
TOTAL:2DRAWING （1）SPOT Ag THICKNESS ［局部D.Ag 厚度］:3.0～8.0μm AMF'7.323.A.2C36MM PLATING UNITMATERIAL[材料]：A194-F.H.NINGBO KANGQIANG ELECTRONICS CO.,LTDTHE NO: 1SOP8L-A8(60×60)LEADFRAME ［引线框架］SIGNER DATE REV NO.QTY DRAWN BY PROCESS CHK'D BY APPROVED BY STANDARD A M F '7.323.A .2C NOTE[技术要求]:1.DIMENSION QUADRANT IS SYMMETRICAL FOR ALL QUADRANTS EXCEPT OTHERWISE SHOWN. [除另有说明，所有象限的尺寸都是对称的。
]2.GENERAL TOLERANCE UNLESS OTHER SPECIFIED:±0.050. [未注尺寸公差为±0.050]3.RADIUS UNLESS OTHER SPECIFIED[未注圆角半径]: R0.127 MAX 4.VERTICAL BURR[垂直毛刺]: 0.025 MAX HORIZONTAL BURR[水平毛刺]: 0.051 MAX 5.MINIMUM LEAD SPACE[最小引线脚间隙]：0.10 MIN 6.LEAD TIP PLANARITY FROM DAM BAR [从中筋到引线内脚的平面度]：±0.1027.PAD FLATNESS[基岛平面度]：0.010/2.540 MAX 8.LEAD TIP FLAT WIDTH[引线内脚平面的宽度]：0.225 MIN 9.CAMBER[侧弯]：0.041 MAX ; COIL SET[卷弯]: 0.508 MAX CROSS BOW[横弯]: 0.254 MAX 10.PAD TILT[基岛倾斜]：0.051/2.540 MAX 11.PAD PLANARITY[基岛平面公差]：±0.10212.COINING DEPTH[精压深度]：0.038 MAX 13.LEAD TWIST[引线脚扭曲]：2.5°MAX 14.FRAME TWIST[引线框扭曲]：0.508 MAX 15.LEAD TILT[引线脚倾斜]：2.5°MAX 16.THE FOURTH DECIMAL PLACE IS USED FOR TOOLING REFERENCE ONLY.[第四位小数仅被用于开引线框架模具时参考的]17.PART TO BE FREE OF RUST,KINKS,BENDS,WRINKLES,SLUG OR TOOL MARKS,OR SCRATCHES DEEPER THAN 0.007. EXT LEAD SPANKING MARK IS IMPOSED 0.254 AWAY FROM DAM BAR. [产品铁锈、 纽绞、弯曲、皱纹、断裂或模具压痕或刮伤程度不超过0.007mm]18.SPOT SILVER,SEMI-BRIGHT.[局部点状镀银，半光亮]19.DIMENSIONS SHOWN FOR INTERNAL LEAD POSITIONS REPRESENT THAT AFTER STAMPING BUT BEFORE COINING [已标出的引线内脚位置的尺寸是冲压之后精压之前的尺寸]20.D/S TOOL MARK ON TIE BAR ONLY,WITH DEPTH [打弯压印,深度]≤0.05121.PLATING AREA[镀银区域]： MIN[最小]2.80×2.70 MAX[最大]3.30×3.1022.DIE PAD SIZE[装片区尺寸]： 1.524×1.524 （60×60）67.000065.600064.000063.000058.150055.000050.150049.625047.525047.000042.150039.000034.150031.000026.150025.625023.525023.000018.150015.000010.15007.0000 2.15001.40001.15000.0000 5.05000.00005.47505.98308.30009.350010.400014.900020.375020.883024.25001.5240±0.0250.40000.4000±0.0251.2700±0.02530°68.500065.400061.500057.600057.200055.885053.500049.400047.325045.500037.500029.500023.325021.500013.5000 5.5000 1.60000.61708.0000±0.0253.18001.90007.600011.720016.800022.5000R0.25001.5240Ø1.5240±0.025Ø1.000025.825049.825057.40005.35004.07001.27505.0750KQ 11ED100601马叶军2010-6-2TOTAL:2DRAWING （1）SPOT Ag THICKNESS ［局部D.Ag 厚度］:3.0～8.0μm AMF'7.323.A.2C36MM UNITMATERIAL[材料]：A194-F.H.NINGBO KANGQIANG ELECTRONICS CO.,LTDTHE NO: 2SOP8L-A8(60×60)LEADFRAME ［引线框架］SIGNER DATE REV NO.QTY DRAWN BY PROCESS CHK'D BY APPROVED BY STANDARD A M F '7.323.A .2C 70.0000±0.051256 PAD / STRIP 29.8000±0.02514.9000±0.0255.3500±0.0769.1500±0.07615*14.9±0.025=223.5000±0.051238.0000±0.102 3.17502.82751.6500 MAX PLATING 1.4000 MIN PLATING & COIN 0.00000.76202.52752.79503.22502.35002.4500 PKG.0.90000.15001.00001.5050 2.1350 1.45001.25000.96500.17700.12700.2500 1.5335 2.13501.9500 PKG.1.5500 MAX PLATING 1.3500 MIN PLATING & COIN 0.74000.42000.20000.00000.7620 1.60004.0000±0.102R0.150045°BURR SIDE [毛刺面]PLATING SIDE [电镀面]45°A A A-A 0.2030±0.0080.1650±0.0250.3450 MIN COIN 0.3100 MIN COINB B B-B 50:10.010~0.03860° 2.4500BURR SIDE [毛刺面] 1.00000.2500 1.9350KQ 11ED100601马叶军2010-6-2TOTAL:2DRAWING （1）SPOT Ag THICKNESS ［局部D.Ag 厚度］:3.0～8.0μm AMF'7.323.A.2C45MM PLATING UNITMATERIAL[材料]：A194-F.H.NINGBO KANGQIANG ELECTRONICS CO.,LTDTHE NO: 1SOP8L-B8(130×95)LEADFRAME ［引线框架］SIGNER DATE REV NO.QTY DRAWN BY PROCESS CHK'D BY APPROVED BY STANDARD A M F '7.323.A .2C NOTE[技术要求]:1.DIMENSION QUADRANT IS SYMMETRICAL FOR ALL QUADRANTS EXCEPT OTHERWISE SHOWN. [除另有说明，所有象限的尺寸都是对称的。
【实验】转基因植物产品检测实验室一览
【关键字】实验转基因植物产品检测实验室一览其他设备：细胞融合仪、核酸提取仪、紫外分光光度计、核酸蛋白检测仪磁力搅拌机杂交仪、-30℃低温冰箱、超低温冰箱、漩涡混合器、超声波细胞粉碎仪、自动恒温酶标。
7 操作步骤7.1 抽样参照 NY/T672 转基因植物及其产品检测通用要求和NY/T673 转基因植物及其产品检测抽样。
7.2 制样参照 NY/T672 转基因植物及其产品检测通用要求和NY/T673 转基因植物及其产品检测抽样（按照GB 5491中四分法制备样品进行送检）。
7.3 DNA模板的制备a称取200-400 mg试样，在液氮中磨碎，装入已经用液氮预冷的1.5 ml离心管中。
b加入1ml预冷至4 ℃的抽提液，剧烈摇动混匀后，在冰上静置5分钟，用13 000 r/min离心机，4 ℃离心15 min，弃去上清液。
c加入600 μl 预热到65 ℃的抽提裂解液，用玻棒搅拌上下颠倒充分混匀，在65 ℃的水浴锅中裂解40 min。
d用13 000 r/min离心机室温离心10 min，将上清液转至另一离心管中，加入5 μl RNase A （10 mg/ml），37 ℃水浴30 min。
e分别用等体积苯酚：氯仿：异戊醇（25：24：1）和氯仿：异戊醇（24：1）各抽提一次。
f用13 000 r/min离心机室温离心10 min，将上清转至另一离心管中。
加入2/3体积异丙醇，1/10 体积3M乙酸钠（pH 5.6），-20 ℃放置2-3 h，充分沉淀DNA。
g13 000 r/min，4 ℃离心15 min，用70%乙醇洗沉淀一次，倒出乙醇，晾干DNA。
加入50 μl TE（pH8.0）溶解DNA。
h把DNA溶液浓度用重蒸馏水调制为100ng/μl，储存于-20 ℃备用。
注意：I 1 g试样（如棉花种子）提取的DNA量应不小于200 μg。
II DNA的OD260/OD280的比值应在1.8左右，且OD260的值应在曲线的最高峰。
2株芝麻真菌病害生防菌的抑菌特征、促生评价及鉴定
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河南农业科学ꎬ２０１９ꎬ４８(１１) :９２￣９８ Ｊｏｕｒｎａｌ ｏｆ Ｈｅｎａｎ Ａｇｒｉｃｕｌｔｕｒａｌ Ｓｃｉｅｎｃｅｓ
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２ 株芝麻真菌病害生防菌的抑菌特征、 促生评价及鉴定
何碧珀ꎬ赵 辉ꎬ刘红彦ꎬ倪云霞ꎬ文 艺ꎬ刘新涛
sesame?biocontrolbacteria?bacillusvelezensis?bacillussubtilis?screening?identification芝麻是世界上重要的优质油料作物?也是我国主要的油料作物之一?其种子油脂含量高且含有丰富的抗氧化物质17?在我国主要产区?芝麻茎点枯病棒孢叶斑病蠕孢叶斑病枯萎病球黑孢叶枯病等发生普遍?对芝麻生产造成了严重的影响8?目前?对芝麻真菌病害的防治主要以化学农药为主911?但随着人们生态环境保护和食品安全意识的不断提高?生物防治因其不易使病原菌产生抗药性?对人畜安全?有利于环境保护等特点?成为植物病害防治的重要发展方向之一?芽孢杆菌具有较强的抗逆能力和安全性?其在生物防治领域作为植物病害生防菌得到了广泛的研究和应用?江苏省农业科学院植物保护研究所筛选的枯草芽孢杆菌bs916?对水稻纹枯病田间防效稳定在6081?已进行农药登记12?北京绿色农华作物科技有限公司以贝莱斯芽孢杆菌bv01发酵液为活性成分研发的杀菌剂?能有效防治小麦赤霉病花生白绢病和花生猝倒病?已获得专利保护13?美国agraquest公司利用枯草芽孢杆菌qst713和qst2808开发的活菌剂serenadetm和sonataas已在美国登记使用?用于防治土传的丝核菌和镰刀菌14?martinez等15用贝莱斯芽孢杆菌ah2制成的杀菌剂?不仅可以有效防治真菌性病害?还可刺激植物生长?目前?关于芽孢杆菌在芝麻真菌病害防治方面的报道较少?且这些报道多数只针对单一病害?本研究以5种芝麻常见病原真菌为靶标?对从采集的412份土壤样品中筛选出的2株生防菌sfb34和sfb109进行了广谱抑菌活性测定和促生评价?并通过形态观察生理生化和分子生物学相结合的方法对菌株进行鉴定?为将菌株作为重要生防因子开发和研究提供科学依据?1材料和方法1
黑莓(黑珀) CM10-CN Accessory Guide
Ethernet Module interfaces with USB Host connector on Single Dock, (871-033-001). (1.5”L x 2.5”W x 1”T).
Non-Powered vehicle holder for hands-free communications.
USB Host and Client Support with bay for charging a spare battery pack. (8.9”L x 4.5”W x 2”T) Requires AC adapter (851-061-502) and country-specific power cord. Optional: Single Dock Ethernet Module (871-238-011).
871-033-021
Single Dock Ethernet Module 871-238-011
Vehicle Holder
805-664-001
Vehicle USB & Power Adapter, 871-037-001 CN50 Series
Vehicle Dock Install Kit
Cabled RS232 or USB Host support is also available. Requires use of RS232 Serial cable (225-737-001) or USB cable (VE011-2016).
Qubit
Qubit® dsDNA HS Assay Kits操作说明书V2【产品名称】通用名称：双链DNA超敏检测试剂盒（荧光法）英文名称：Qubit® dsDNA HS Assay Kits【包装规格】100人份&500人份/盒【预期用途】Nanodrop系列微量分光光度计采用目前最常见的紫外吸光法对待测样品的浓度及纯度进行检测，由于它会检测吸光度在260nm处所有物质的吸光值（如DNA、RNA、降解核酸和游离核苷酸等其他杂质），因此读数并不是十分准确。
而荧光计通过荧光染料与特定目标分子结合后，检测其荧光强度来测目标分子的浓度，因此其定量结果一般低于A260nm处的读数，但是更为准确。
另外，准确的DNA定量对于许多后续应用而言都至关重要。
Nanodrop系列全波长微量分光光度计无法精确测定5ng/ul以下的DNA浓度，然而，许多DNA样品又恰在这个范围之内。
在检测双链DNA时，荧光计的检测极限可达0.5pg/ul。
此时，更为灵敏的荧光计似乎是个更好的选择。
荧光计和相应的定量试剂盒，能快速灵敏、精确测定DNA的浓度。
本试剂将被用于成本昂贵的下游实验:qPCR 、PCR克隆、转染和新一代测序等精密测定的实验，精准定量范围10 pg /µL至100ng/µL。
【检验原理】本试剂盒利用Picogreen DNA染料能够特异性结合双链DNA，而不与蛋白质、RNA、盐离子结合的原理，通过激发光照射，Qubit检测仪收集发射光，特异的分析样品中双链DNA 的实际含量，能够排除样品中蛋白质、RNA、盐离子等物质的干扰，从而对双链DNA进行精准的定量。
【主要组成成份】①不同批次的试剂不可混合使用。
【储存条件及有效期】2-8℃保存；避免反复冻融；有效期6个月。
【适用仪器】荧光仪（Qubit 2.0 ，Qubit 3.0等荧光仪）【需要但试剂盒没有提供】一次性无尘手套、Qubit® assay tubes (500 tubes, Life Technologies, Cat. no. Q32856)或者Axygen® PCR-05-C tubes （VWR, part no. 10011-830）【检验方法】1. 实验准备：1.1 准备好检测所需要的0.5ml的薄壁离心管（n=待测样品数+2 standards）；1.2 在薄壁离心管的管盖处进行标记样品的编号，切不可在管的侧壁进行标记。