lab5---Generating a JavaServer Faces CRUD Application from a Database
(生物类研究生必学)DNAMAN、DNAstar、primer5.0、Endnote常用功能介绍
EndNote
EndNote 是THOMSON 公司推出的最受欢迎的一 款产品,是文献管理软件中的佼佼者。其具有海量 文献信息的管理、全文管理、笔记管理、自动编排 论文或书籍的参考文献、利用杂志全文模版撰写论 文、统计与分析等功能,强烈推荐!!!
养成良好的阅读习惯,不管做实验还是写文章将会 事半功倍。
8. 引物5′ 端和中间△G值应该相对较高,而3′ 端 △G值较低。
△G值是指DNA 双链形成所需的自由能,它反 映了双链结构内部碱基对的相对稳定性,△G值越 大,则双链越稳定。应当选用5′ 端和中间△G值相 对较高,而3′ 端△G值较低(绝对值不超过9)的 引物。引物3′ 端的△G 值过高,容易在错配位点形 成双链结构并引发DNA 聚合反应。
DNAstar
DNAstar软件,即著名的Lasergene Suite,功能
主要有:序列的格式转换,序列拼接和重叠克隆群 的处理;基因寻找;蛋白质结构域的查找;多重序 列的比较和两两序列比较;寡核苷酸设计(PCR引 物,测序引物,探针)。由EditSeq 、MegAlign 、GeneQuest 、MapDraw 、PrimerSelect 、 Protean 、SeqMan II七个模块组成。
类型⇒选择相应的要打开的文件
序列格式转换 ① 载入序列 ② 主菜单栏⇒序列⇒显示序列
寻找酶切位点 ① 载入序列 ② 主菜单栏⇒限制性酶切⇒限制性酶切分析
序列比对 ① 主菜单栏⇒序列⇒比对⇒多重序列比对 ② 工具栏⇒多重序列比对
DNAstar功能强大,这里主要讲EditSeq。
运行EndNote后,出现的第一个界面如下:
我们可以新建一个数据库:
如何将文献导入数据库
1. 电脑里的本地文献 选择Import File出现以下界面:
LabWindows CVI 8.0.1 发布说明说明书
LabWindows/CVI Release Notes™™Version 8.0.1These release notes introduce LabWindows™/CVI™ 8.0.1. Refer to this document for system requirements, installation and activation instructions, and information about new features in LabWindows/CVI.ContentsMinimum System Requirements (2)Installation Instructions (2)Activation Instructions (2)What’s New in LabWindows/CVI? (3)Download LabWindows/CVI DLLs to Real-Time Targets (3)Create Visual Studio 2005 Projects That Use LabWindows/CVI Libraries (3)Call .NET Assemblies (4)Create Optimized Code (4)Add Tabs to Graphical User Interfaces (4)Use Updated Graph Control Features (4)Distribute LabWindows/CVI Applications (4)View the Source Code Browser in the Workspace Window (5)Include New Cell Types in the Table Control (5)Use New Advanced Analysis Library Functions (5)Customize Menu Bars (5)Enhance Toolbars (5)Record Macros (6)Auto-Indent Source Files (6)Enable Documentation Tooltips for User-Defined Functions (6)Include Alpha-Channel Data in Image Files (6)Experience Improved DIAdem Connectivity Library Performance (6)Use the Enhanced Instrument I/O Assistant (7)Create IVI Class Drivers with the IVI Custom Class Driver Wizard (7)LabWindows/CVI Resources (7)Minimum System RequirementsTo run LabWindows/CVI, you must have the following items:•Personal computer using a Pentium 600 or higher microprocessor•Windows 2000/XP•800 × 600 resolution (or higher) video adapter•Minimum of 128 MB of RAM, 256 MB recommended•150 MB free hard disk space for full installation•Microsoft-compatible mouseInstallation InstructionsComplete the following steps to install LabWindows/CVI 8.0.1:install version 8.0.1 in a different directory or uninstall the earlier version before installingversion 8.0.1. If you have LabWindows/CVI 8.0 installed on your computer, you can installversion 8.0.1 in the same directory. In this case, LabWindows/CVI 8.0.1 overwritesLabWindows/CVI 8.0.1.Insert the LabWindows/CVI CD into the CD drive. If the CD does not run automatically,open Windows Explorer, right-click the CD drive icon, and select AutoPlay.2.Select Install LabWindows/CVI on the National Instruments LabWindows/CVI screen.bWindows/CVI prompts you to enter your user information. Select one of thefollowing options:•Install this product using the following serial number•Install this product for evaluationIf you choose to enter a serial number, enter the serial number found on your Certificateof Ownership card. LabWindows/CVI automatically uses this serial number when yourun the NI Activation Wizard.4.Continue to follow the instructions on the screen.Activation InstructionsComplete the following steps to activate LabWindows/CVI 8.0.1:1.When you launch LabWindows/CVI for the first time after installation,LabWindows/CVI displays the Evaluation License dialog box. Click Activate to launchthe NI Activation Wizard.LabWindows/CVI Release © National Instruments Corporation 3LabWindows/CVI Release Notes 2.In the NI Activation Wizard, select one of the following methods to acquire the activationcode and click Next .•Automatically activate through a secure Internet connection —Automatically receive an activation code.•Use a Web browser, email client, telephone, or fax machine to acquire anactivation code —Contact National Instruments to obtain an activation code. Youwill then enter the code in the NI Activation Wizard to activate LabWindows/CVI.•Apply a 20-character activation code previously acquired for thiscomputer —Enter a code you have obtained to activate LabWindows/CVI.Note The remaining instructions assume you select the Automatically activate through a secure Internet connection option. If you select an alternate option, follow theinstructions on the screen to complete product activation.3.Enter or verify your serial number. Click Next .4.Continue to follow the instructions on the screen.5.When you successfully activate LabWindows/CVI, click Finish .What’s New in LabWindows/CVI?This section includes information about changes and enhancements in LabWindows/CVI.Download LabWindows/CVI DLLs to Real-Time TargetsYou can use LabWindows/CVI to create a DLL that you can download to an RT target directly from LabWindows/CVI. After LabWindows/CVI downloads the DLL, you can debug the DLL on the RT target or deploy the DLL to a production RT target. You must have theLabWindows/CVI 8.0 Real-Time Module installed on your computer to use this feature.For more information about downloading DLLs, refer to the Programmer Reference»Creating and Downloading DLLs to Real-Time Targets section of the LabWindows/CVIHelp .Create Visual Studio 2005 Projects That Use LabWindows/CVI LibrariesUse the LabWindows/CVI Application Wizard to create a new Visual C++ project that uses LabWindows/CVI libraries. You also can use the LabWindows/CVI Conversion Wizard to convert an existing LabWindows/CVI project to a Visual C++ project. To use these wizards, you must have installed Microsoft Visual Studio 2005 or later (Standard edition or higher).For more information about creating Visual Studio 2005 projects that use LabWindows/CVI libraries, refer to the Using LabWindows/CVI»Wizards and Tools»Creating Visual Studio 2005 Projects section of the LabWindows/CVI Help.Call .NET AssembliesUse the .NET Library to call .NET assemblies. LabWindows/CVI also provides the CreateLabWindows/CVI .NET Controller dialog box, which you can use to generate an instrument driver that serves as a wrapper for a .NET assembly.For more information about using .NET assemblies in LabWindows/CVI, including sample code, refer to the Library Reference».NET Library section of the LabWindows/CVI Help.Create Optimized CodeYou can use an external optimizing compiler to compile LabWindows/CVI code from within the LabWindows/CVI environment. To compile your code using an external optimizingcompiler, you must create a compiler configuration for that compiler. LabWindows/CVIprovides configuration templates for Microsoft Visual C++, Borland, and Intel compilers.For more information about creating optimized code, refer to the Programmer Reference»Creating Optimized Code section of the LabWindows/CVI Help.Add Tabs to Graphical User InterfacesUse the tab control to organize user interface panels into multiple tabbed pages. As with all other LabWindows/CVI controls, you can create and modify tab controls programmatically or in the User Interface Editor.For more information about the tab control, refer to the Library Reference»User InterfaceLibrary»Controls»Control Types»Tab Control section of the LabWindows/CVI Help.Use Updated Graph Control FeaturesYou can use the graph control features for the following tasks:•Add annotations to specified data points•Use absolute and relative time formats for graph axes•Change plot settings interactively with the graph legend•Convert pixels to graph units•Use multi-line text in custom axis label text stringsFor more information about the graph control features, refer to the Library Reference»User Interface Library»Controls»Control Types»Graph Control section of the LabWindows/CVI Help.Distribute LabWindows/CVI ApplicationsLabWindows/CVI provides a redesigned way to distribute applications. You now cancreate a distribution that includes not only the LabWindows/CVI application but also itsdependencies. Use the new distribution creation feature to include NI product drivers andcustom merge modules in installers.LabWindows/CVI Release © National Instruments Corporation 5LabWindows/CVI Release Notes For more information about the new distribution system, refer to the UsingLabWindows/CVI»Managing Projects»Building a Project»Distributing Applications section of the LabWindows/CVI Help .View the Source Code Browser in the Workspace WindowYou can view and interact with the Source Code Browser from within the Workspace window. LabWindows/CVI displays the Source Code Browser at the bottom of the Workspace window.Include New Cell Types in the Table ControlThe table control now supports ring, combo box, and button cells. The table control continues to support numeric, picture, and string cells, as it has in previous versions.For more information about the table control, refer to the Library Reference»User Interface Library»Controls»Control Types»Table Control section of the LabWindows/CVI Help .Use New Advanced Analysis Library FunctionsThe Advanced Analysis Library has been improved to include new curve fitting and windowing functions, high-performance linear algebra functions, and enhancements to two-dimensional Fast Fourier Transform (FFT) analysis and other signal processing features.Note The Advanced Analysis Library is available in the LabWindows/CVI Full Development System only.For more information about the updated Advanced Analysis Library, refer to the Library Reference»Advanced Analysis Library section of the LabWindows/CVI Help .Customize Menu BarsYou can customize menu bars by adding images to menu items and specifying a font for menu bars. For more information about customizing menu bars, refer to the UsingLabWindows/CVI»Developing a Graphical User Interface»Creating a GUI»Menu Bars»Creating a Menu Bar topic in the LabWindows/CVI Help .Enhance ToolbarsYou can add pull-down menus, string controls, and color numeric controls to the toolbar. You also can include multiple toolbars on a single panel. The updated toolbar instrument driver provides a move handle, which you can place on a toolbar to allow a user to dock and undock the toolbar and to move the toolbar from one panel to another.For more information about the toolbar, refer to the Tools Library»Toolbar Control section of the LabWindows/CVI Help.Record MacrosYou can record macros in the Source window to save and repeat a series of actions, such as keystrokes and menu selections. For more information about macros, refer to the UsingLabWindows/CVI»Writing Source Code»Recording Macros topic in the LabWindows/CVIHelp.Auto-Indent Source FilesLabWindows/CVI automatically indents source code based on the bracket style preferences you specify. The auto-indent feature is turned on by default. You can set auto-indent options in the Editor Preferences dialog box.Enable Documentation Tooltips for User-Defined FunctionsYou can add special tags to functions you define to make documentation for these functions available throughout your project. For more information about adding the documentationtags, refer to the Using LabWindows/CVI»Writing Source Code»Editing a Source File»Documenting Source Code topic in the LabWindows/CVI Help.Include Alpha-Channel Data in Image FilesYou can set alpha-channel data in bitmaps you use in LabWindows/CVI to create partiallytransparent images. To set alpha-channel data, you must use the .png file format.LabWindows/CVI includes the following functions specifically for .png support:•NewBitmapEx•SetBitmapDataEx•GetBitmapDataEx•GetBitmapInfoEx•AllocBitmapDataEx•SaveBitmapToPNGFileFor more information about alpha-channel data and .png files, refer to the LibraryReference»User Interface Library»Overview»Common User Interface Objects»UsingBitmap Objects topic in the LabWindows/CVI Help.Experience Improved DIAdem Connectivity Library Performance The updated DIAdem Connectivity Library now features improved performance forappending data to an existing file. For more information about the DIAdem ConnectivityLibrary, refer to the Library Reference»DIAdem Connectivity Library section of theLabWindows/CVI Help.LabWindows/CVI Release Use the Enhanced Instrument I/O AssistantThe Instrument I/O Assistant now features improved code generation capabilities. You also can configure instrument inputs at run time using the assistant. For more information about the Instrument I/O Assistant, refer to the Using LabWindows/CVI»Wizards and Tools»Creating an Instrument I/O Task section of the LabWindows/CVI Help.Create IVI Class Drivers with the IVI Custom Class Driver Wizard Use the new IVI Custom Class Driver Wizard to help you create IVI class drivers forinstrument classes not defined by the IVI Foundation. With custom class drivers, you canreplace one instrument belonging to that class with another instrument without modifyingyour test applications. This new wizard automatically generates source (.c), include (.h), and function panel (.fp) files for your custom IVI class driver.For more information about the wizard, refer to the Using LabWindows/CVI»Wizards andTools»Creating an IVI Custom Class Driver section of the LabWindows/CVI Help. LabWindows/CVI ResourcesHow do I get started?Read the Getting Started with LabWindows/CVI manual, which provides a tutorial forlearning basic LabWindows/CVI program development techniques.Are there known issues or late-breaking information?Refer to the LabWindows/CVI Readme, which you can access from Start»Programs»National Instruments»LabWindows CVI 8.0.1»LabWindows CVI Readme. The readme file contains information about known issues.Where can I find reference information?The LabWindows/CVI Help contains complete reference information. The help file includes the following sections:•Using LabWindows/CVI describes the LabWindows/CVI development environment and features.•Library Reference provides information about functions you can call inLabWindows/CVI.•Programmer Reference provides information to help you develop applications.•Tools Library provides information about the LabWindows/CVI Toolslib controls.Use the Search tab in the LabWindows/CVI Help to quickly locate specific information. For information about how to use the search functionality, refer to the Using Help»SearchingHelp topic in the LabWindows/CVI Help.© National Instruments Corporation7LabWindows/CVI Release NotesWhere can I find examples?Find examples with the NI Example Finder, which you can access by selecting Help»Find Examples.Is there a list of LabWindows/CVI documentation?The Guide to LabWindows/CVI Documentation topic provides links to LabWindows/CVIdocumentation, including manuals, application notes, and white papers. You can access the Guide to LabWindows/CVI Documentation topic through the LabWindows/CVI Help.Where else can I go for LabWindows/CVI information?Visit the LabWindows/CVI Web site and NI Developer Zone at for the mostup-to-date information about LabWindows/CVI.National Instruments, NI, , and LabVIEW are trademarks of National Instruments Corporation.Refer to the Terms of Use section on /legal for more information about NationalInstruments trademarks. Other product and company names mentioned herein are trademarks or tradenames of their respective companies. For patents covering National Instruments products, refer to theappropriate location: Help»Patents in your software, the patents.txt file on your CD, or/patents. For a listing of the copyrights, conditions, and disclaimers regardingcomponents used in USI (Xerces C++, ICU, and HDF5), refer to the USICopyrights.chm.© 2003–2006 National Instruments Corporation. All rights reserved.373607D-01Mar06。
Feng Zhang实验室CRISPR设计工具在iPSCs基因组编辑中的应用说明说明书
Genome editing in iPSCsCrispr designWe used the Feng Zhang lab CRISPR Design tool () to design the guide RNA. The guide RNA should bind as closely as possible to the target nucleotide (ideally between 2-10 bp).To correct a mutation, the mutated DNA sequence should be used for the gRNA design.To introduce a mutation, the wild-type DNA sequence should be used for the gRNA design.For each selected gRNA, order two oligonucleotides - the sense and antisense of the guide sequence. The BbsI restriction site overhangs (depends on the plasmid) are as follows:Fw oligo: 5’-CACC[G]”guide_sense_sequence”-3’Rv oligo: 5’-AAAC”guide_antisense_sequence”[C]-3’Resuspend each oligo in distilled water to 100µM.Note: only add the G and the C in square brackets if the guide doesn't start with a G. This is necessary for optimal transcription initiation from the U6 promoter.Typically, we design two gRNAs and test the cutting efficiency before the HDR experiment.Design of ssDNA Oligo HDR donor templateWe use a mix of up to 4 HDR donor ssDNA Oligos (100nt long) depending on the targeted mutation, the gRNA binding site and the PAM sequence.We will design the donor OligosCloning of guide RNA in the pSpCas9(BB)-2A-GFP vector (Addgene: px458)Step1. Oligo phosphorylation and annealing:To phosphorylate and anneal each pair of oligos, combine the following in a 0.2ml tube:1 µl oligo Fw (100µM in water)1 µl oligo Rv (100µM in water)1 µl 10X T4 Ligation Buffer (NEB)6.5 µl ddH2 O0.5 µl T4 PNK (NEB)Total volume = 10 µl totalIncubate37°C 30 min95°C 5 minRamp down to 25°C at 5°C/minStep 2. Digestion and ligation:1 µl pX330 or other backbone vector pSpCas9(BB)-2A-GFP (100ng/ul)2 µl of diluted oligo duplex from step 1 (diluted 1:250 in water)2 µl 10X FastDigest Buffer1 µl DTT (10mM)1 µl ATP (10mM)1 µl FastDigest BbsI (ThermoFisher)0.5 µl T7 DNA ligase (NEB)11.5 µl ddH2OTotal volume = 20 µlIncubate:37°C 5 min23°C 5 minCycle 6 times (total run time ~1h)Step 3. Transformation:•Add 5µl of ligation mix to 50 µl E.coli strain e.g One Shot competent cells•Incubate on ice for 20 minutes•Heat-shock the cells at 42°C for 45 seconds•Cool on ice for 2 minutes•Add 500µl of SOC media and incubate at 37°C with shaking for 30 minutes•Plate 100µl of the transformed bacteria on LB-agar + 100µg/ml ampicillin plates.•Incubate at 37°C overnight.Step 4. E coli colony screening for gRNAThe next morning, pick 5 colonies and incubate overnight in 5ml LB + 100µg/ml ampicilline at 37°C with shaking. After 8 hours, isolate the DNA with a DNA miniprep kit and send for Sanger sequencing with the hU6_Fw primer (5’-ACTATCATATGCTTACCGTAAC-3’).iPSC cultureReagents:•Essential 8 (Life Technologies, A14666SA)•Matrigel™ hESC-Qualified Matrix (Corning, cat. no. 354277)•Gentle Dissociation Solution (Stem Cell Technologies 07174)•Y-27632-HCl (Biorbyt, cat. no. orb154626)Thawing hiPSC:•hiPSC should be either generated in-house or can be obtained from available depositories e.g Wicell, Coriell or the Stanford CVI iPSC Biobank.•Remove vial from liquid nitrogen, place in 37 °C water bath until only a sliver of ice remains.Transfer the vial content dropwise (~1 mL) to a 15 mL conical tube filled with 4 ml of pre-warmed E8 supplemented with 2.5µM Y-27632 (E8 + iRock)•Centrifuge at 200 g for 4 min. Carefully aspirate the supernatant. Re-suspend the cell pellet in 2 mL of E8 + iRock and transfer to 1 well of a Matrigel-coated 6-well plate •Change media every 24 h with fresh E8. Cells should be 70-80% confluent in 3-4 days Passage of hiPSC:•Ideally cells should have reached 70-80% confluence in 3-4 days (adjust split ratio accordingly, typically 1/6-1/12).•Aspirate the E8 culture medium.•Add 2 mL per well of Gentle Dissociation Solution, incubate for 6-8 min at RT (in hood) •Whilst waiting, aspirate medium from Matrigel-coated plates and replace with 1 mL of E8Y.•Aspirate Gentle Dissociation Solution from each well.•Add 1 mL of E8 + iRock medium to the well. Gently detach the colonies by scraping with a serological pipette or a cell scraper. Add 6 mL of E8 + iRock for a 1:6 split.•Mix gently and transfer 1 mL in each well of the 6-well plate (2ml per well total volume). NOTE: We aim to keep the pluripotent cells in the logarithmic growth phase. Cells should not be allowed to become more than 90% confluent.TransfectionThe day before transfection, split the cells 1:2/1:3. Cell should be 50-60% confluent the next day: •Remove the medium•Wash once with PBS•Add 2.0ml Gentle Dissociation Solution•Incubate 5 min 37°C•Gently pipette up and down to dissociate the cells•Plate in a previously coated new well in E8 + iRock.On the day of transfection:•Replace media with fresh E8 media (1.0 mL/well).•For each well of a 6-well plate, prepare 4 separate reactions•Prepare the reactions by adding the reagents in the order shown:(i)Set up the CRISPR-Cas9-gRNA rxn:Mix A:o200 µL of Opti-MEMo10 µL of Lipofectamine Stem reagentMix B:o200 µL of Opti-MEMo1µg of CRISPR/Cas9 vector (pSpCas9(BB)-2A-GFP)Combine mix A & B – mix well and incubate for 15min @ RT(ii) A separate reaction is set up for the ssDNA donor oligos:Mix C:o200 µL of Opti-MEMo7.5 µL of RNAi max reagentMix D:o200 µL of Opti-MEMo 4 µg of ssDNA donor mix (if using 4 oligos, use 1µg each)Combine mix C & D – mix well and incubate for 15min @ RT•Add 400µl transfection mix of A+B and 400µl transfection mix of C+D to one well•Place the cells back in the incubator•After 4 hours, aspirate the transfection media from each well and replace with 2ml of fresh E8 supplemented with E8 + iRock•16-24h later, check the transfected cells under a fluorescent microscope for GFP+. Typically, the transfection efficiency is 5%-30% depending on the iPSC line. We FACs sort the cells 24-36h post transfection.FACS GFP+ cells•Aspirate the media•Wash once with PBS•Add 2.0 ml TrypLE express•Incubate 5-6 min 37°C until cells have detached – mix gently with a P1000 pipette to break down the cells to single cells•Add 4ml E8 + iRock•Transfer to a 15ml tube•Centrifuge 5 min @200g room temperature•Discard the supernatant and re-suspend the cells in 0.4 ml E8 + iRock•Filter the suspension through a 35-µm mesh Corning™ Falcon™ Test Tube with Cell Strainer Snap Cap•Prepare a 15ml collection tube containing 6ml E8 + iRock•Sort GFP-expressing cells using FACS sorter with a 100-µm nozzle – typically we sort 12,000 cells•Plate sorted cells in 6-well plates at a density of 2000 cells/well in E8 + 2.5µM iRcokIsolation of iPSC ClonesUsually 8-10 days after sorting, single iPSC colonies are large enough to be picked. We usually pick 30-40 clones that are clearly isolated from 3-6 wells of a 6-well plate.•Aspirate media and add 3ml of fresh E8 + iROCK media per well at least 2h before picking.•Manually pick individual iPSC clones (we use a P200 pipette set at 100µl) using a stereo-microscope located inside a cell culture hood, and transfer each clone to a 1.5ml tube.•Pipet up and down 2-3 times to partially dissociate the clone and transfer 90 µl of cell suspension into a separate well of a 24-well plate (Matrigel-coated) containing 500µL of E8+iRock. Savethe remaining 10µl of cells suspension for HDR screening by direct PCR (the samples can bestored at -20o C).•Allow the cells to attach for 24–48 h and then add 500µl E8 media. Feed the cells with fresh E8 media every other day.Genomic DNA isolationProtocol adapted from Phire Animal Tissue Direct PCR Kit (Thermo Fisher; Cat#: F140WH).•For each clone, dilute 0.5µl of DNA Release Additive in 19.5µl Dilution Buffer. We make a master mix for all the clones. Add 20µl of the master mix to each tube containing the ~10 µl of residual cell suspension from the clone picking step.•Mix well and incubate at room temperature for 10 minutes.•Then heat the samples at 98°C for 2 minutes.•Add 25µl of molecular grade water and centrifuge at top speed for 1 min at room temperature to allow for cell debris sedimentation. Use 3µl of the resulting solution as a template in the PCRreaction.Direct PCRPerform PCR using PrimeSTAR GXL DNA Polymerase (Clontech) and primers that amplify a region of 500nt around the target nucleotide. For each sample combine the following:13.5µl ddH2O (for 3µl template DNA)5µl PrimeSTAR GXL Buffer 5X2µl dNTP (2.5mM each)0.5 µl 10µM Fw Primer0.5 µl 10µM Rv Primer0.5 µl PrimeSTAR GXL DNA Polymerase3.0 µl of cell sampleTotal volume = 25 µlSet up the following program on the thermocycler:2 min 98°C10 sec 98°C15 sec 62°C20 sec 68°C(repeat X40)2 min 68°Chold 4°CSanger SequencingFirst, run 5µl of the PCR reaction on a 1% agarose gel to verify genomic DNA amplification.Once verified, the unpurified PCR samples (~20 µl) are sent out for sequencing using either the forward or the reverse primer.。
reporting summary for nature research说明书
|April2018Corresponding author(s):Reporting SummaryNature Research wishes to improve the reproducibility of the work that we publish.This form provides structure for consistency and transparency in reporting.For further information on Nature Research policies,see Authors &Referees and the Editorial Policy Checklist .Statistical parametersWhen statistical analyses are reported,confirm that the following items are present in the relevant location (e.g.figure legend,table legend,main text,or Methods section).The exact sample size (n )for each experimental group/condition,given as a discrete number and unit of measurementAn indication of whether measurements were taken from distinct samples or whether the same sample was measured repeatedlyThe statistical test(s)used AND whether they are one-or two-sidedOnly common tests should be described solely by name; describe more complex techniques in the Methods section.A description of all covariates testedA description of any assumptions or corrections,such as tests of normality and adjustment for multiple comparisonsA full description of the statistics including central tendency (e.g.means)or other basic estimates (e.g.regression coefficient)ANDvariation (e.g.standard deviation)or associated estimates of uncertainty (e.g.confidence intervals)For null hypothesis testing,the test statistic (e.g.F ,t ,r )with confidence intervals,effect sizes,degrees of freedom and P value notedGive P values as exact values whenever suitable.For Bayesian analysis,information on the choice of priors and Markov chain Monte Carlo settingsFor hierarchical and complex designs,identification of the appropriate level for tests and full reporting of outcomesEstimates of effect sizes (e.g.Cohen's d ,Pearson's r ),indicating how they were calculatedClearly defined error barsState explicitly what error bars represent (e.g. SD, SE, CI)Our web collection on statistics for biologists may be useful.Software and codePolicy information about availability of computer codeData collection Data analysis For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature,software must be made available to editors/reviewers upon request.We strongly encourage code deposition in a community repository (e.g.GitHub).See the Nature Research guidelines for submitting code &software for further information.Keiji KubaBio-Rad Image lab Touch software (version 2.1.0.35),Thermal Cycler Dice Real Time System Software (version 5.11C for TP900),BDCellQuest ProTM (version 6.0),Nikon NIS-Elements (version 3.22.00),Bio-Rad Microplate Manager Software (version 6.3),FinePoint(version 2.8.0.12146),FACS Calibur (Becton Dickinson)Bio-Rad Image lab (version 5.2),Thermal Cycler Dice Real Time System Software (version 5.11C for TP900),Excel (version 2108),Prism(version 9.2.0),BD CellQuest ProTM (version 6.0),FlowJo (version 10.8.0),FinePoint (version 2.8.0.12146)April 2018DataPolicy information about availability of dataAll manuscripts must include a data availability statement .This statement should provide the following information,where applicable:-Accession codes,unique identifiers,or web links for publicly available datasets-A list of figures that have associated raw data-A description of any restrictions on data availabilityField-specific reportingPlease select the best fit for your research.If you are not sure,read the appropriate sections before making your selection.Lifesciences Behavioural &socialsciences Ecological,evolutionary &environmental sciencesFor a reference copy of the document with all sections,see /authors/policies/ReportingSummary-flat.pdfLife sciences study designAll studies must disclose on these points even when the disclosure is negative.Sample size Data exclusions Replication Randomization Blinding Reporting for specific materials,systems and methodsUnique biological materialsPolicy information about availability of materialsObtaining unique materials AntibodiesAntibodies used The data that support the findings of this study are provided in the Article and its Supplementary Information.Source data are provided with this paper.GenBank accession code for SARSCoV-2,HKU-001a is MT230904.1.We performed the power analysis to predetermine sample size.We did not exclude the data.One independent experiment was performed for Figures 1b,2b-d,5b,5c and Extended Data Figures 1a-h,3c,3d,6b,6f.Independentexperiments were performed two times for Figures 1c,2f-j,3c-l,4b-k,5a,5d-h and Extended Data Figures 1i,1j,3a,3b,5a-f,6a,6c-e,7a-g,8a-f and three times for Figures 6b-g and Extended Data Figures 2a-e,4a-h,and consistent results were obtained.The animals were allocated into experimental group in random.The investigators were blinded to group allocation during data collection and analysis.We confirm that the unique materials in this study are available from us.For western blotting,we used anti-mouse ACE2antibody (Crackower,M.et al.,Nature,2002,doi:10.1038/nature00786),anti-hamster GAPDH antibody (GeneTex,GTX100118),anti-SARS-CoV/SARS-CoV-2NP antibody (Chan,J.F.et al.,Clin Infect Dis,2020,doi:10.1093/cid/ciaa325),anti-human ACE2antibody (R&D systems,MAB9331),anti-beta-actin antibody (Sigma,A5316,batchApril 2018Validation Eukaryotic cell linesPolicy information about cell linesCell line source(s)Authentication Mycoplasma contamination Commonly misidentified lines(See ICLAC register)Animals and other organismsPolicy information about studies involving animals ;ARRIVE guidelines recommended for reporting animal researchLaboratory animals Wild animals Field-collected samples Flow CytometryPlotsConfirm that:axislabels state the marker and fluorochrome used (e.g.CD4-FITC).axis scales are clearly visible.Include numbers alongaxes only for bottom left plot of group (a 'group'is an analysis of identical markers).plots are contour plots with outliers or pseudocolor plots.numerical value for number of cells or percentage (with statistics)is provided.MethodologySample preparation Instrument Software Cell population abundance number 123M4876)and anti-human IgG antibody (MBL,103R,lot186).For in vitro binding assay,we used anti-human ACE2antibody (Novus Biological,SN0754,NBP2-67692,lotHN0420),anti-mouseACE2antibody (Crackower,M.et al.,Nature 2002,doi:10.1038/nature00786),anti-human IgG antibody (MBL,103R,lot186),anti-B38-CAP polyclonal antibody (Minato,et al.,Nat Commun,2020,doi:10.1038/s41467-020-14867-z),FITC-conjugated humanIgG-specific polyclonal antibody (Jackson ImmunoResearch,#109-095-088,lot137124)and Fc antibody (JacksonImmunoResearch,#109-035-098,lot146365).Validation information of each antibody is as follows:anti-mouse ACE2antibody (Crackower,M.et al.,Nature 2002,doi:10.1038/nature00786),anti-hamster GAPDH antibody (https:///PDF/Download?catno=GTX100118),anti-SARS-CoV/SARS-CoV-2NP antibody (Chan,J.F.et al.,Clin Infect Dis,2020,doi:10.1093/cid/ciaa325),anti-human ACE2antibody (https:///pdfs/datasheets/mab9331.pdf?v=20211006&_ga=2.210780560.420495370.1633586814-1097249821.1598599156),anti-beta-actin antibody (https:///deepweb/assets/sigmaaldrich/product/documents/863/388/a5316blot.pdf),anti-human ACE2antibody(https:///PDFs4/NBP2-67692.pdf),anti-human IgG antibody (https://ruo.mbl.co.jp/bio/dtl/A/?pcd=103R),anti-B38-CAP polyclonal antibody (Minato,et al.,Nat Commun,2020,doi:10.1038/s41467-020-14867-z),FITC-conjugatedhuman IgG-specific polyclonal antibody (https:///catalog/products/109-095-088)and Fc antibody(https:///catalog/products/109-035-098).Vero E6cells (CRL-1586)and Caco2cells (HTB-37)were obtained from ATCC.Vero E6/TMPRSS2(JCRB1819)were obtainedfrom JCRB Cell Bank.Expi293F cells were obtained from Thermo Fisher Scientific (A14635).Vero E6cells and Caco2cells were authenticated with STR profiling by ATCC.VeroE6/TMPRSS2cells was authenticated byJCRB Cell Bank,but no information of technique to authenticate is available.Expi293F cells was not authenticated.Not tested for Mycoplasma contaminationNoneAll animal experiments conformed to the Guide for the Care and Use of Laboratory Animals,Eighth Edition,updated by the USNational Research Council Committee in 2011,and approvals of the experiments were granted by the ethics review board ofAkita University,NIBIOHN,the University of Tokyo or the University of Hong Kong.We used 3,6or 10week-old male or female Syrian hamsters and 3-4month-old male human-ACE2transgenic mice.Three tofour month-old male or female C57BL/6J mice were used to backcross human-ACE2transgenic mice.No wild animals were used in this study.No field collected samples were used in this study.FACS Calibur (Becton Dickinson)FlowJo v10.8softwareFive thousand cells per analysis and 100%purity of live Vero E6cells。
《2024年利用软件PrimerPremier5.0进行PCR引物设计的研究》范文
《利用软件PrimerPremier5.0进行PCR引物设计的研究》篇一一、引言随着分子生物学技术的飞速发展,聚合酶链式反应(PCR)已成为实验室研究中不可或缺的技术之一。
PCR引物设计是PCR实验成功的关键步骤之一,其准确性直接影响到PCR的扩增效率和特异性。
因此,选择合适的引物设计软件对于PCR实验的成功至关重要。
本文将介绍如何利用PrimerPremier5.0软件进行PCR引物设计的研究。
二、PrimerPremier5.0软件简介PrimerPremier5.0是一款功能强大的引物设计软件,具有直观的用户界面和丰富的功能,可帮助研究人员快速设计高质量的PCR引物。
该软件支持多种PCR类型,包括常规PCR、实时荧光定量PCR等,可满足不同实验需求。
三、PCR引物设计流程1. 输入目标序列:打开PrimerPremier5.0软件,输入需要设计引物的目标序列。
目标序列可以是基因片段、cDNA等。
2. 设置引物参数:根据实验需求,设置引物的长度、GC含量、退火温度等参数。
PrimerPremier5.0软件会根据这些参数,自动筛选出符合要求的引物序列。
3. 设计引物:在软件中,通过设置不同的引物组合,生成多个潜在的引物序列。
这些引物序列的特异性和扩增效率可通过软件进行评估和预测。
4. 筛选引物:根据实验需求和引物评估结果,选择最合适的引物序列。
在选择过程中,需要考虑引物的特异性、扩增效率、引物间及引物与模板间的互补性等因素。
5. 验证引物:将设计的引物序列导入PCR实验中,进行验证。
通过PCR实验结果,评估引物的特异性和扩增效率。
四、PrimerPremier5.0软件的优势1. 直观的用户界面:PrimerPremier5.0软件具有直观的用户界面,使得用户可以轻松地完成引物设计过程。
2. 丰富的功能:该软件支持多种PCR类型,可满足不同实验需求。
同时,该软件还具有引物评估和预测功能,可帮助用户选择最合适的引物序列。
Brightspace MyLab和Mastering标准配置指南(系统管理员)说明书
Brightspace MyLab and Mastering Standard Configuration Guide for System AdministratorsThis document describes the configuration for enabling the Pearson MyLab and Mastering solution within the Brightspace (formerly known as Desire2Learn or D2L) Learning Management System, including:1.Requesting MyLab and Mastering remote plug-in credentialsHow to request the Consumer Key and Secret for the MyLab and Mastering toolconfiguration2.Configuring the MyLab and Mastering remote plug-ins at the Brightspaceinstance/system levelHow to configure the MyLab & Mastering tools within the Brightspace LearningManagement System at the instance/system level3.Mapping roles and organizations unit typesHow to map Brightspace and Pearson roles and organization unit types to ensurethat students can successfully register for and access a Pearson course4.Enabling the MyLab and Mastering Tools widget at the course levelHow to enable the MyLab & Mastering Tools widget within a course5.Troubleshooting common errorsHow to resolve common errors and Pearson resources for additional assistanceMyLab and Mastering is only available for the Brightspace SaaS-based solution.SECTION 1: Requesting MyLab and Mastering credentialsThis section describes how to request the consumer key and secret for the MyLab and Mastering remote plug-in configuration. A single set of credentials is requested per Brightspace installation. These should be requested by the Brightspace system administrator who configures the MyLab and Mastering remote plug-in.Credential Request1. Navigate to the following URL:/keymaster/ui/u/index?consumer=d2l2. Enter the following on the page:∙Your Institution∙Installation Type (Select Production, Testing, or Other)∙Email Address∙First Name∙Last Name∙Phone Number (Optional)∙Job Title (Optional)3. Enter the verification text for reCaptcha.4. Click Submit. A confirmation page with the consumer key and secret generated for your institution appears.5. Check your email for a secure message containing the configuration information. You are prompted to create an account with Pearson's secure email provider, Sendinc, to access the email message containing these credentials.SECTION 2: Configuring the MyLab and Mastering remote plug-ins at the Brightspace instance/system levelThis section describes how to configure the MyLab & Mastering tools in Brightspace at the instance/system level.ConfigurationCreate the Remote Plug-InsNote: Before completing the following steps, verify your institution is running a Brightspace SaaS-based solution.1. Navigate to the Brightspace Admin Tools view.2. Click Remote Plugins.3. Click New Remote Plugin to add the external tools required for the MyLab andMastering integration.4. Enter the following on the Create a New Remote Plugin page:∙Launch Point: https:///highlander/api/o/lti/links∙Secret: Consumer secret obtained for your institution in Section 1, Step 5.∙Key: Consumer key obtained for your institution in Section 1, Step 5. Note the format of this value is ∙Name: MyLab and MasteringEnter the name exactly as it appears in this guide, with no spaces before or after the name. You can enter spaces in between the words.∙Description: /keymaster/ui/u/index?consumer=d2l ∙Small Icon: /highlander/resources/img/links/Pearson-Logo-50x50.png∙Large Icon: [Leave this value blank.]∙System Test: [Leave this value blank.]∙Plug-in Type: Course Builder∙Make the link available to all Org Units and descendants of that Org Unit that will use the MyLab and Mastering integration.Note: The secure email you receive when you request your credentials lists the key ahead of the secret, but, when you configure your remote plugins in Brightspace, the area for the secret appears before the key. The key appears in the format .5. Click Save.6. Click New Remote Plugin to add the second plugin required for the MyLab andMastering integration.7. Enter the following on the New Remote Plug-in page:∙Launch Point: https:///highlander/api/o/lti/minitoolswidget ∙Secret: Consumer secret obtained for your institution in Section 1, Step 5.∙Key: Consumer key obtained for your institution in Section 1, Step 5. Note the format of this value is ∙Name: MyLab & Mastering LinksEnter the name exactly as it appears in this guide, with no spaces before or after the name. You can enter spaces between words.∙Description: /keymaster/ui/u/index?consumer=d2l ∙Small Icon: [Leave this value blank.]∙Large Icon: [Leave this value blank.]∙System Test: [Leave this value blank.]∙Plug-in Type: Widget∙Make the link available to all Org Units and descendants of that Org Unit that will use the MyLab and Mastering integration.8. Click Save.9. Navigate to the Brightspace Admin Tools view, and click External Learning Tools.a.Click MyLab and Mastering launch. Under Security Settings, click Use toolprovider security settings and make sure these check boxes are selected: o Send tool consumer information to tool providero Send context information to tool providero Send LTI user ID and LTI role list to tool providero Send user name to tool providerb.Click MyLab and Mastering Links launch. Under Security Settings, click Use linksecurity settings and make sure these check boxes are selected:o Send tool consumer information to tool providero Send context information to tool providero Send LTI user ID and LTI role list to tool providero Send user name to tool provider10. From the Brightspace Admin Tools view, click Manage Extensibility.a.Search for the Pearson’s MyLab and Mastering v1.0 application in the Extensibilitylist. If you do not see Pearson application, click the Force Application Sync button to get the latest Brightspace Application updates, which include Pearson's MyLab and Mastering v1.0.b.Enable the Pearson’s MyLab and Maste ring application, if not enabled by default.Note:If your institution’s Brightspace instance is running under https, ensure that your DOME->Settings value for OrgHasSSLEnabled is set to true/ON for the Pearson Course Builder Tool to display properly.SECTION 3: Mapping roles and organizations unit typesThis section describes how to map Brightspace and Pearson roles and organization unit types to ensure that students can successfully register for and access a Pearson course.Map Roles1.Navigate to the Brightspace Admin Tools view.2.Click IMS Configuration.3.Click the Roles tab.4.Ensure that the role types are mapped as follows:Brightspace Role Mapped Roles (IMS standard)Instructor Faculty, Instructor, StaffStudent Student, LearnerSuper Admin Instructor, AdministratorAdministrator Instructor, AdministratorMap Organization Unit Types1.Navigate to the Brightspace Admin Tools view.2.Click IMS Configuration.3.Click the Org Unit Types tab.4.Ensure that the organization unit types are mapped as follows:Brightspace Organization Unit Type Mapped Organization Unit Type (IMS standard)Course Template CourseTemplate Course Offering CourseOffering Group GroupSection CourseSectionSECTION 4: Enabling the MyLab & Mastering Tools widget at the course levelThis section describes options for enabling the MyLab & Mastering Tools widget within a course.OptionsThe approach depends on the policies and procedures of an individual institution. Possible approaches include:1.If all courses that fall under a particular course template will use the MyLab andMastering integration, the widget can be added to the Template home page to beinherited by all the Course Offerings created from that template.2.If allowed by the institution, an instructor who wants to incorporate the MyLab andMastering integration into a course can create a new Course home page and add the MyLab & Mastering widget to the page.If editing the Course home page is not supported for instructors at the institution, an instructor who wants to incorporate the MyLab and Mastering integration in a course must submit a request through the standard campus processes for requesting new course widgets.SECTION 5: Troubleshooting common errorsIf you encounter errors, check that:∙The current course start and end dates are included for the course or that defaults are used.∙There are no spaces preceding or trailing the course name.∙There are no special characters in the course name or in the user's course role description:o UTF8 characters related to non-English characters (acute, grave, cedilla, etc.) can often be used with a workaround by the Product team that usually takestwo to three weeks to implement.o There is no workaround for special characters in English (parenthesis, double dash, etc.)∙There are no special characters in the institution name.o There is currently no workaround for special characters in the institution name.∙The institution name is less than 32 characters.∙The course name is less than 32 characters.∙If you try to add a component link and see a message saying: “An error occurred: Unable to add or update links in your course” make sure you updated the securitysettings.How do I find information regarding Pearson system issues?The Pearson System Status site provides up-to-date performance information for a subset of Pearson Higher Education products. The goal of this site is to improve the customer experience by providing updates about system disruptions and outages.https:///getsupport/s/article/Pearson-System-Status-SiteNetwork provider: If your Brightspace environment is self-hosted and you plan to change your network provider, please contact us first, so that we can update the necessary SSL certificates to avoid issues with the integration.Installation support: If you have questions or receive errors when installing and testing this integration, please contact ********************* for support.End-user support: After installation, instructors and students can contact Pearson Support at https:///getsupport/s/. You can contact a support technician or search the Knowledge Base for relevant articles by entering MLMBrightspace in the search box.。
labsolutions使用流程
labsolutions使用流程English Response:Introduction.LabSolutions is a comprehensive software suite designed to enhance the efficiency and accuracy of laboratory analyses. Developed by Shimadzu, a leading manufacturer of analytical instrumentation, it empowers users with advanced features and intuitive functionality.Key Features.Data Acquisition and Control: LabSolutions seamlessly integrates with a wide range of analytical instruments, enabling seamless data acquisition and control. Users can remotely monitor and adjust instrument parameters, ensuring optimal performance and minimizing the risk of errors.Data Processing and Analysis: Robust data processingtools allow users to perform a comprehensive range of operations, including peak integration, smoothing, and baseline correction. Advanced statistical analysis capabilities enable the extraction of meaningful information from complex data sets.Chromatography Management: LabSolutions provides comprehensive chromatography management features, including peak identification, retention time alignment, and qualitative and quantitative analysis. It supports various chromatography techniques, including HPLC, GC, LC-MS, and GC-MS.Method Development and Validation: LabSolutions streamlines method development and validation processes. Users can create and optimize methods in a user-friendly environment, ensuring compliance with regulatory standards and enhancing analytical accuracy.LIMS Integration: The software can integrate with Laboratory Information Management Systems (LIMS), enabling seamless data transfer and eliminating the risk of manualerrors.User Interface: LabSolutions features an intuitive and customizable user interface that simplifies data handling and analysis. Its configurable layouts and customizable toolbars allow users to tailor the software to their specific workflows.Benefits.Enhanced efficiency and productivity.Improved data accuracy and reliability.Time savings and reduced operating costs.Simplified compliance with regulatory standards.Increased confidence in analytical results.中文回答:简介。
Infoprint 250 導入と計画の手引き 第 7 章ホスト
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MyLab辅助功能最佳实践指南说明书
MyLab Accessibility Best Practices GuideStriving to ensure every learner succeeds MyLab course materials Alternative course materials Accessible eTextbooks Alternate format text AccessText Network Braille and large print MyLab accessibility features Turning on Accessibility Platform and navigation Assignments and assessments Question bank for homework and tests Custom Question Builder Microsoft PowerPoint decks Alternate text Videos Discipline-specific media In your MyLab course: New considerations Support and documentation VPATs and other accessibility status documents Table of Contents112222344456789910101010Introduction/OverviewStriving to ensure every learner succeedsMyLab course materialsEmpowering learners will always be central to Pearson’s mission and values. That includes designing instructional content for MyLab® to be fully accessible to students with disabilities while continually improving usability.By honoring the following best practices together, we can enable a wider array of students to thrive through their learning journey — and prepare everyone to prosper.Pearson’s investment in accessible product design and remediation is significant and ongoing as we strive to meet and exceed Web Content Accessibility Guidelines (WCAG 2.1 AA standards) for all our educational materials including those designed for MyLab courses.If for any reason any Pearson eTextbook doesn’t meet a student’s need, Pearson is prepared to provide other course material options. To save everyone time and effort, we’ve partnered with top providers and accessibility experts to expand our capabilities.Alternative course materialsAccessible eTextbooksPearson’s newest eTextbook platform, Pearson+, supports the majority of WCAG 2.1 AA standards and we are continuously updating to improve both accessibility and usability as well as compatibility with assistive technologies. Keep in mind that the most recent edition of each title will provide the most accessible user experience.Pearson+ eTextbooks can be accessed in two ways:1. Using a MyLab courseIf your course uses Pearson MyLab online learning platforms, students may already have access to an accessible eTextbook. To open, go to the MyLab course menu in your browser and select the eTextbook option.2. Logging in to Pearson+ directlyFor classes that do not use MyLab, students can log in to Pearson+ to access eTextbooks from a computer or mobile device (iOS and Android), even when offline.Please email us at ****************************** for title-specific details or if you encounter any issues.Alternate format textIf a Pearson+ eTextbook is not fully accessible for a student based on their needs, an alternate format text can be found within the Pearson VitalSource accessible digital textbooks library.You may purchase Pearson titles as eTextbooks from VitalSource and get instant access to eTextbooks through the VitalSource Bookshelf platform.Learn more about accessible digital eTextbooks from Pearson and get answers to common questions about them.AccessText NetworkPearson partners with the AccessText Network to ensure that students with print-based disabilities that would be better addressed by a non-digital textbook also have that option. Upon request and at no added cost, additional Pearson titles are available for qualified students who buy or rent our print or eTextbook versions through AccessText Network, a clearinghouse for files from all major higher education publishers.A disability services representative must make these title requests from the school who must register with the AccessText Network. The alternative text file will be delivered typically as an untagged PDF (meaning not fully accessible for digital consumption).This method is ideal for:• Schools intending to produce their own braille or large format documents• Students requiring a printed textbook versus digital book as a result of a disability Braille and large printPearson and Allyant, the leading provider of accessible textbook formats in North America, partnered to significantly reduce the turnaround time and cost of providing top-selling Pearson titles in braille and reflowed large print.T-Base can deliver top-selling braille or reflowed large-print textbooks within 10 business days at a substantial cost reduction to institutions. Purchasing accessible textbooks through this new, more efficient process enables educators to focus on helping students succeed in their studies. Explore the ever-expanding Allyant Catalog. Order textbooks in braille or reflowed large print by emailing Allyant at: **************** or calling: 1-800-563-0668.Platform and navigationAll MyLab website pages are screen reader and keyboard-accessible including student pages like Calendar, Homework and Tests, Results, Announcements, and Study Plan.The platform’s interface works with common screen magnifiers. Browser- or device-based contrast settings are respected.*Note: When the student is using keyboard-only navigation or assistive technology within MyLab, the Accessibility Mode is required to complete assignments. (Explore the command-line language students can use to type symbols when Accessibility Mode is turned on.)Assignments and assessments Question bank for homework and testsWhen giving students with disabilities any assignments, be sure their eTextbooks display a copyright year of 2014 or later, and keep in mind that all Pearson business products published in 2016 or later also have accessible eTextbooks.Contact ****************************** for questions about other eTextbooks. Accessible questions are noted with an icon to help instructors select accessible assessmentsand require alternative text.Pearson is actively and consistently increasing the number of accessible questions.Accessible questions and items are:• Screen reader and keyboard accessible• Compatible with common screen magnifiers• Contrast-optimized for vision-impaired access• Created with accessible media such as video with captions and/or video descriptions and images with alternative textCustom Question BuilderWhen editing existing questions or composing your own, remember to:• Add alternative text for images• Use only captioned media• Format fonts for optimal readability• Weigh the use of static vs algorithmic questions for ease of editing and to accommodate student needs• Want to learn more? Watch our MyLab Create Your Own Questions video.• Use questions from other texts and the new enhanced book search. Watch our MyLab Enhanced Book Search video.Microsoft PowerPoint decksMicrosoft PowerPoint decks copyrighted 2018 or later are accessible and include: • Slides that use a clear, simple language and layout• Slides that use accessible fonts of a minimum size• Defined reading order• Accessible color contrast ratios (4.5:1) for text and images of text and color combinations that can be read by students with color blindness• Alternative text descriptions for images• Accessible math, where applicable• Slide titles in the title placeholder for each slide that are unique and concise• Meaningful text or raw URLs to describe hyperlinks• Lists that are built as structured lists• Columns that are created with defined reading order• Tables that are created with PowerPoint’s table feature, are simple grids with predictable rows and columns, and are free of merged cells• Slides that are free of background images and flickering imagesAlternative Text• Alternative text (“Alt Text”) is a written description that conveys visual content of images to students who are blind or have low vision. Pearson eTextbooks with a copyright year of 2018 or later have alternative text for images.• Alternative text is read aloud by screen readers and other assistive technology devices.For complex images, a long description may also be provided via hyperlink to fullydescribe the image.VideosVideos:• Are navigable using screen readers and keyboards• Offer closed captioning in most Business, Reading & Writing, and Math & Statistics products. Note: MIS titles and products released prior to 2010 may vary.• Provide access to certain transcripts via the video player. (See the Further Problem Solving example.) Activate this feature in “Settings.” Some transcripts are available as supplemental materials within the course and do not require a request.• Will include audio descriptions in future releases of visual details on screen that are not apparent from the audio alone.In your MyLab course: New considerations Support and documentationVPATs and other accessibility status documents• Link an HTML eBook directly to its own tab.• Use screen-reader compatible questions. If using Skill Builder, select only the screen reader questions.• Extend testing time by 50% by using the individual student settings.• VPATs and Accessibility Conformance and Remediation Forms (ACC&R) are available for many products upon request and organized and described by feature and status. • To request this information, contact Pearson Disability Support .• Learn more about Pearson’s Accessibility online or contact your Pearson Sales Representative.Discipline-specific mediaMany MyLab products feature discipline-specific media (e.g.: animations, simulations, experiments, flashcards, etc.). For updated details on their accessibility, refer to discipline-specific accessibility status flyers.。
android java opencv facedetectyn 用法
android java opencv facedetectyn用法在Android平台上使用OpenCV库进行人脸识别是一项涉及多个步骤的任务。
以下是一个关于如何使用OpenCV的Java接口进行人脸检测的基本指南。
1. 导入OpenCV库首先,你需要在你的Android项目中导入OpenCV库。
这通常通过在你的build.gradle 文件中添加依赖来完成。
确保你已经下载并安装了OpenCV的Android SDK。
2. 初始化OpenCV在你的应用程序中,你需要初始化OpenCV库。
这通常在主活动(MainActivity)的onCreate方法中完成。
你可以调用OpenCVLoader.initDebug()来初始化OpenCV库。
3. 加载人脸检测器OpenCV提供了多种人脸检测方法,其中最常见的是Haar特征级联分类器。
你需要加载一个预训练的XML文件,该文件包含了用于人脸检测的特征和参数。
javaCascadeClassifier faceDetector = new CascadeClassifier(getContext().getResources().getIdentifier("lbpcascade_frontal face", "raw", getContext().getPackageName()));if (faceDetector.empty()) {Log.e(TAG, "Failed to load cascade classifier");// 处理加载失败的情况}4. 处理图像接下来,你需要从摄像头或图像库获取图像,并将其转换为OpenCV可以处理的格式(通常是Mat对象)。
5. 进行人脸检测使用加载的人脸检测器在图像上检测人脸。
这通常通过调用detectMultiScale方法完成。
javaMatOfRect faceDetections = new MatOfRect();faceDetector.detectMultiScale(grayImage, faceDetections);其中grayImage是一个灰度图像,因为Haar特征级联分类器通常在灰度图像上运行得更快。
LabTech 10.5及以上版本:Webroot LabTech插件V2.5商业用户说明书
What’s New LabTech plugin V2.5 For LabTech Version 10.5 and aboveDocument Version 2.0.2Table of ContentsIntroduction (2)Installation & Compatibility (2)Relocated Webroot Settings Dashboard (2)New Enhanced Settings Dashboard (3)Default Webroot Keycode (3)Unique Identifier (3)Enable additional alerts when computers are in an “Attention Required” state (3)Enable reboot pop-ups when computers are in an “Attention Required” state (4)Enable additional alerts when computers stay infected for longer than xx hours (4)Enable alerts when endpoint is stale for longer than xx days (4)Enable expired license alerts (4)New Enhanced Interactive Home Dashboard (5)Webroot Client level changes (6)Client Settings (6)Detail View (7)My Webroot Anywhere (7)Webroot Computer level changes (8)Other Miscellaneous Changes (8)Help Content (8)LabTech Health Reports and AV Dashboard Integration (9)Additional Monitors (10)Additional Scripts (11)Omissions (11)IntroductionWith LabTech plugin version 2.5, customisability of the UI has been greatly enhanced to make day-to-day tasks easier. With new monitors to provide time saving out the box options for our customers and usability enhancements throughout. Each tab now features help content, accessible by the question mark symbol and pop-up tool tips have been added wherever it makes sense.Installation & CompatibilityVersion 2.5 of the plugin is designed for LabTech Control Centre 10.5 and above. When upgrading or installing for the first time, please use the installer provided (do not use the DLL). This ensures all the right additional components are loaded to support the enhanced user interface.Relocated Webroot Settings DashboardTo make navigation simpler, we have relocated the Settings Dashboard to a new location inside the Global System Integration Dashboard, easily accessible by clicking on the Webroot icon in the main LabTech navigation bar, located on the top of the main screen.New Enhanced Settings DashboardThe new home for the Settings tab! We have added a number of new alerts to help our customers manage and automate their environments right out the box.Default Webroot KeycodeThis keycode entry is only for Webroot customers who use the same Webroot Site key for all LabTech Clients. If you are creating new sites within the Webroot Global Site Manager, site keycodes must be entered at LabTech Client level.Unique IdentifierWhen enabled will add a unique string of characters after the computer name in the Webroot web console to help avoid duplicate computer names.Enable additional alerts when computers are in an “Attention Required” state When the Webroot agent detects a threat, it will block the threat. Most threats, such as real-time or inactive threats are removed in under 1 minute. Some threats require a clean scan before the endpoint is declared malware free. Sometimes, threats are too deeply embedded in the system to be removed immediately and WSA will require a reboot to clean the infection. After the usual daily scan and reboot, most infections are automatically and safely removed without any intervention.To keep the malware reporting noise down to a minimum, we have created a new “Attention Required” flag specifically designed for MSP environments. This flag is raised if an endpoint remains infected after 2 contiguous 12 hour checks. If the endpoint is rebooted or performs a scan at the point during any of the checks, the counter will be reset for another 12 hours. In practice, the “Attention Required” flag will be true (1) if the endpoint remains infected after about 36 hours. This ensures the endpoint has gone through at least 1 reboot/scan cycle before raising the Attention Required flag. The MSP can choose to take either manual or automatic action if they wish, such as initiating another scan or to inform the end user to reboot. Some actions such as running a cleaning scan, or user reboot request may be automated.Important Note: The Attention Required flag is distinctly different than the “Needs Attention” state in the Webroot Console, which is set as soon as an infection is detected. Each indicator works independently.Enable reboot pop-ups when computers are in an “Attention Required” stateIn some cases for the Webroot agent to fully remediate a persistent threat, or to declare an endpoint free of malware, one or more reboot cycles may be needed. If users do not shutdown their PCs overnight then it could remain infected. Enabling the “reboot pop-up alert” after the “Attention Required” flag is set will ensure a pop-up alert is sent to the end users device at midday, informing the user to reboot.Enable additional alerts when computers stay infected for longer than xx hours When a Webroot agent stays infected for longer than the amount of hours defined (2, 8, 12, 24) an additional alert will be triggered via the “Webroot - Active Infection” Internal Monitor. This alert is useful for customers who need to be informed of persistent infections as quickly as possible.Enable alerts when endpoint is stale for longer than xx daysIf a Webroot agent fails to successfully check-in to the Webroot cloud for longer than the days defined (7, 15, 30, 60, 90) an alert will be triggered via the “Webroot - Stale Agents” Internal Monitor.Enable expired license alertsWhen a Webroot agent’s license expires it will trigger an alert via the “Webroot - License Expired” Internal Monitor.New Enhanced Interactive Home Dashboard Have it the way you want! The new Home dashboard brings in customization of the main display. This is the first of a series of planned enhancements to ease everyday use of the plugin.If issues are detected, selected fields will change to red.Each cell is interactive and can take you to Client, Locationor PC level for ease of management.Columns are fully customizable and you can select justthe columns you need to run your day to dayoperations. Each change is saved and if the applicationor the window is closed all settings will remain as saved(except sorting).Additional columns can be added fromthe Column Chooser menu – right clickto activate.Webroot Client level changesAll client level tabs have been updated, these are listed below.Client SettingsThe Webroot Client setting have an updated layout, updated tooltips, new help screen and updated wording for clarity. The Optional Webroot Group setting has been moved off to the right.Detail ViewThe new detailed view site level dashboard brings in customization of the main display, allowing you to set all the data columns your own way.My Webroot AnywhereThe Webroot Console is now even easier to access then before. The email address of the logged-in LabTech user is automatically passed to the integrated Webroot Console viewer.Webroot Computer level changesWe have added a new command button and changed the layout and the wording of the display for ease of use. The new “Run Customer Support Diagnostics” button will run a script against the remote agent, which will download the wsablogs.exe file from Webroot and run it on the remote computer using the logged-in LabTech user’s email address as the ID.Other Miscellaneous ChangesHelp ContentWe have added help buttons at every tab that explains each function at every level. Just click on the question mark icon to open up the help content.LabTech Health Reports and AV Dashboard IntegrationWe have integrated the total number of scans performed in the LabTech Health reports.E ach of the scan scripts have had the script stat “VirusScanRunStat” record in each run.NOTE: Only the scans run by the LT based scripts are counted. Normal WSAB daily scans are not included.In addition, we have integrated “Last Threat Fo u nd” and “Action Taken” results in the LabTech AV Dashboard. S tats are recorded to the inherent “virus tables” in LabTech. This is additional to recording the stats on the Webroot custom plugin tables.We have added 5 new customizable monitors to ease day-to-day automation: 1-Attention Required2-Reboot Needed3-Active Infection4-Stale Agents5-License ExpiredWe have added 2 new customizable scripts to ease day-to-day automation: 1-Customer Support Diagnostics2-Reboot NeededNOTE: The new monitors are only designed to be used in conjunction with the “Run Customer Support Diagnostics” button and “Reboot Needed” monitor, and not on their own.OmissionsWhile we have taken every care to keep the information within this document as accurate as possible, omissions or inaccuracies could occur. If you spot any, please report it to your Webroot representative.。
人工智能训练师(3级)理论知识复习题
输入层
输出层
激活函数
22
在知识图谱中,用于表示实体间关系的边通常具有什么类型?
有向边
无向边
加权边
线性边
23
类脑计算的主要目标是:
模拟人类的大脑功能 b) 创建一个全新的智能
创建一个全新的智能生物
提高计算机的运算速度
优化网络安全性
24
在模式识别中,特征选择的目标是:
降低维度
增加噪声
增强数据
数据预处理
任务目标
项目预算
数据来源
项目截止时间
11
在评估人工智能系统时,下列哪个因素是最重要的评估指标?
a) 系统的准确性
系统的准确性
系统的响应速度
系统的外观设计
系统的品牌知名度
12
评估人工智能系统时,下列哪个因素可以衡量系统的可靠性和鲁棒性?
系统的安全性
系统的用户友好性
系统的社会影响
系统的数据处理能力
13
当模型在验证集上不能达到理想的评估指标时,( )。
加强与行业人员的联系
提高技术实施效率
提升数据分析准确性
减少团队协作难度
4
人工智能是通过什么来呈现人类智能的技术?
计算机程序
机械装置
化学合成物
电子设备
5
人工智能的研究内容主要包括什么?
计算机实现智能的原理
网络安全技术
建筑设计原理
动物行为研究方法
6
人工智能科学是一门研究、开发和应用智能体的( )。
跨学科领域
57
VMAF是用于评估视频质量的指标,它基于以下哪个原理进行评估?
人眼感知模型
编码效率
数据压缩算法
系统中心虚拟机管理器与Hyper-V基础知识说明书
Course 10981A: Infrastructure Provisioning with System Center Virtual Machine ManagerCourse OutlineModule 1: Overview of Hyper-V and Virtual Machine ManagerBefore Virtual Machine Manager can be used to manage the virtualized environment you should understand some of the key concepts and features in both Hyper-V and Virtual Machine Manager. This includes the various networking and storage features in Virtual Machine Manger and how Virtual Machine Manager User Roles and Run As Account can be used to control access to the resources being managed. You should also understand how you can use Windows PowerShell with Virtual Machine Manager and how you can generate PowerShell Cmdlets for many tasks in the Virtual Machine Manager Console.Lessons∙Overview of Hyper-V∙Overview of Virtual Machine ManagerLab : Completing the Fabric for the VMM environmentAfter completing this module, students will be able to:∙Describe the key components and features in Hyper-V.∙Describe the key components and features in Virtual Machine Manager.Module 2: Configuring High Availability for Virtual Machine Manager and Hyper-VIn most enterprise virtualized environments high availability is a key function that is implemented for business-critical workloads. This ensures workloads remain available at all times and also provides load-balancing capabilities. For this reason it is important that you understand how high availability can be configured for the Hyper-V Hosts, Virtual Machine Manager Management Server and the Virtual Machine Manager database. In this module you will learn about the clustering requirements for Virtual Machine Manager. You will also learn how to configure SQL AlwaysOn for the Virtual Machine Manager database. Finally, you will learn how to configure clustering for Hyper-V.Lessons∙Clustering requirements for Virtual Machine Manager∙Configuring SQL AlwaysOn for the Virtual Machine Manager Database∙Hyper-V Host ClusteringLab : Implementing High Availability for Virtual Machine ManagerAfter completing this module, students will be able to:∙Describe the clustering requirements Virtual Machine Manager.∙Configure SQL AlwaysOn for the Virtual Machine Manager database.∙Configure Hyper-V clustering.Module 3: Updating Virtual Machine Manager and Fabric ServersKeeping the IT environment up to date in an enterprise is paramount from both a security and a performance perspective. Proper update management also helps ensure that systems remain available by applying patches that improve the applications running on them. In the virtualized environment updatemanagement is just as important, especially when there are multiple System Center components deployed. In this module you will learn how Virtual Machine Manager integrates with Windows Server Update Services to manage the updates on Virtual Machine Manager Fabric infrastructure servers such as the Virtual Machine Manager Management Server and Library Server. You will also learn the considerations that should be taken into account and service order that should be applied when updating Virtual Machine Manager when you have other System Center components deployed.Lessons∙Understanding the Servicing Order for System Center∙Performing the manual update process in Virtual Machine Manager∙Patching the Virtual Machine Manager Fabric with Windows Server Update ServicesLab : Updating the Virtual Machine Manager environmentAfter completing this module, students will be able to:∙Describe the servicing order when updating System Center components.∙Update Virtual Machine Manager.∙Update the Virtual Machine Manager Fabric.Module 4: Configuring Networking in Virtual Machine ManagerNetworking in Virtual Machine Manager has been extended extensively within the last two iterations of System Center. For example, in System Center 2012 SP1 Virtual Machine Manager, the ability to deploy third-party switch extensions such as Cisco was added. In System Center 2012 R2 Virtual Machine Manager, Top-of-rack (TOR) switch integration was added. It is important that you not only understand the basic concepts of networking in Virtual Machine Manager but that you also know how to configure some of the advanced network settings. This knowledge will prove invaluable when configuring Virtual Machine Manager in an enterprise environment, especially where networking spans multiples sites and/or locations. In this module you will learn some of the key networking fundamentals in Virtual Machine Manager before moving onto advanced networking features such as Port Profiles, Top-of-rack switches and Windows Server Gateways.Lessons∙Overview of Networking in Virtual Machine Manager∙Logical Networks∙Uplink and Virtual Port Profiles∙Logical Switches∙Virtual Machine Networks∙Software Defined Networking (optional)Lab : Configuring Networking in VMMAfter completing this module, students will be able to:∙Describe the core networking features in Virtual Machine Manager.∙Create logical networks, network sites and IP pools.∙Configure Uplink Port Profiles and Virtual Port Profiles.∙Configure Logical Switches.∙Configure VM Networks.∙Understand Software Defined Networking in Virtual Machine Manager (optional).Module 5: Configuring Storage in Virtual Machine Manager Virtual Machine Manager provides support for both Block Storage and File Storage. Block Storage can include Fibre Channel, iSCSI and Serial Attached SCSI (SAS). File storage includes network file shares that can be located on a Windows File Server or on third party Network Attached Storage (NAS) devices provided by vendors such as NetApp or EMC. When configuring storage in Virtual Machine Manager you need to understand how it is discovered, classified, provisioned, allocated and decommissioned. In this module you will learn about the various storage options that are available in Hyper-V and Virtual Machine Manager. You will also learn how storage is discovered and classified. Finally, you will learn how to configure and manage storage in Virtual Machine Manager.Lessons∙Storage options available in Hyper-V and Virtual Machine Manager∙Understanding Storage in Virtual Machine Manager∙What Storage will be managed?∙Configuring and Managing Storage in Virtual Machine ManagerLab : Provisioning and configuring Storage in Virtual Machine ManagerAfter completing this module, students will be able to:∙Describe the storage options available in Virtual Machine Manager.∙Understand storage in Virtual Machine Manager.∙Understand what storage will be managed in Virtual Machine Manager.∙Configure and manage storage in Virtual Machine Manager.Module 6: Managing Clouds in Virtual Machine Manager One of Virtual Machine Managers most powerful features is its ability to create and manage Private Clouds including the resources made available to them and controlling access to Virtual Machines and Services deployed within them. Before provisioning a Private Cloud in Virtual Machine Manager it is important that you understand the key concepts around Cloud Computing such the difference between Private, Public and Hybrid Clouds. When provisioning a Private Cloud it is also important that you configure the resources available to the cloud appropriately. This includes the Library resources, Virtual Machines and Services that are deployed to the cloud. In this module you will learn some of the key concepts in Cloud computing. You will also learn how to create and manage a Private Cloud in Virtual Machine Manager including how resources, capacity and capability are configured for the cloud. Finally, you will learn how access to the Private Cloud and its associated resources is configured.Lessons∙Introduction to Clouds∙Creating and Managing a Private Cloud in Virtual Machine Manager∙Working with User Roles in Virtual Machine ManagerLab : Managing Clouds in Virtual Machine ManagerAfter completing this module, students will be able to:∙Describe the key concepts in Cloud Computing.∙Create and manage Clouds in Virtual Machine Manager.∙Create and configure User Roles in Virtual Machine Manager.Module 7: Configuring and Managing the Virtual Machine Manager Library and Library Objects The Virtual Machine Manager Library provides access to file-based resources such as sysprep images, virtual hard disks and ISO files. This makes provisioning Virtual Machines and Services much quicker and easier as the Library acts as a catalog of resources that are available at all times. It is important that you understand how to configure and manage the Virtual Machine Manager Library especially when you have multiple Library servers in your environment. In this module you will learn about the Virtual Machine Manager Library structure and how Libraries are synchronized. You will also learn about equivalence objects with the Library and how library objects can be tagged and versioned.Lessons∙Overview of the Virtual Machine Manager Library∙Managing LibrariesLab : Configuring and Managing the Virtual Machine Manager LibraryAfter completing this module, students will be able to:∙Describe the Virtual Machine Manager Library.∙Configure and manager the Virtual Machine Manager Library.Module 8: Preparing the Windows Operating System for deployment in Virtual Machine Manager There are a number of methods that can be used to prepare an Operating System for deployment in Virtual Machine Manager. For example, you can use Sysprep to prepare an Operating System which then generalizes the image so that it can be re-used indefinitely. You can also use a Windows Imaging Format (WIM) file to create a .VHD. It is important that you understand the various methods in which an Operating System can be prepared for deployment so that you can use the best method for your environment. In this module you will learn how to use Sysprep and WIM to prepare Operating System images for deployment with Virtual Machine Manager. You will also learn the architecture of virtual machines in relation to physical computers including how application settings are affected in a virtualized environment. Finally, you will learn about the preparations that must be performed when deploying bare-metal Hyper-V Hosts and Scale-out File Servers.Lessons∙Preparing a Windows Operating System∙Virtual Machine Architecture∙Preparations required for Bare Metal deployments of Hyper-V and Scale-out File ServersLab : Preparing the Windows Operating SystemAfter completing this module, students will be able to:∙Prepare a Windows Operating System for deployment in Virtual Machine Manager.∙Understand Virtual Machine Architecture.∙Prepare for a bare-metal deployment of Hyper-V and Sale-out File Server.Module 9: Virtual Machine and Service Templates Templates in Virtual Machine Manager provide a method of standardizing the deployment of Virtual Machines and Services thereby saving time and ensuring that when deploying them, they are configured appropriately. When creating a Virtual Machine or Service Template there are many properties that can be configured such as the hardware, Operating System and applications that will be deployed, so it is important that you understand how these properties are configured. In addition, when creating Virtual Machine Templates you can also apply Hardware, Guest OS and Application Profiles which can be used to apply standard settings for Virtual Machines and Services deployed with Virtual Machine Manager. In this module you will learn how to configure Virtual Machine and Service Templates. You will also learn how to create hardware and Guest OS Profiles to help standardize the deployment of Virtual Machines and Services. You will also learn how to configure, deploy and update Service Templates including how Service Templates are used in the realworld.Lessons∙Overview of Virtual Machine Templates∙Overview of Service Template TemplatesLab : Deploying Virtual Machines and ServicesAfter completing this module, students will be able to:∙Configure Virtual Machine Templates.∙Configure Service Templates.Module 10: System Center Integration Virtual Machine Manager, as with other System Center components can be integrated with other components to provide additional features and manageability. For example when Virtual Machine Manager is integrated with Operations Manager you can monitor the Virtual Machine Manager environment and be alerted when Operations Manager detects problems such as a pending hard disk failure in the Virtual Machine Manager fabric. By integrating Virtual Machine Manager with Orchestrator you can also automate tasks in Virtual Machine Manager such as deploying a Virtual Machine or a Service. In this module you will learn how integration between Virtual Machine Manager and Operations Manager is configured and what benefits this brings. You will also learn how integration between Virtual Machine Manager and Orchestrator is configured including the benefits this brings.Lessons∙Monitoring the Virtual Machine Manager environment with Operations Manager∙Automation with OrchestratorLab : Responding to Alerts using OrchestratorAfter completing this module, students will be able to:∙Monitor the Virtual Machine Manager environment with Operations Manager.∙Automate the Virtual Machine Manager environment with Orchestrator.Module 11: Windows Azure Pack Windows Azure Pack is a no-cost Azure solution for the datacenter. By using Windows Azure Pack and Virtual Machine Manager you can offer cloud-based services such as Virtual Machine Clouds, Web Site Clouds and SQL Server databases from a secure Self-Service environment hosted in your datacenter. In this module you will learn about the key features of Windows Azure Pack including its architecture and the Tenant and Administrator Portals that provide access to the Windows Azure Pack features. You will also learn about the various deployment methods for Windows Azure Pack including how to perform an express deployment. Finally, you will learn how to configure VM Clouds, User Accounts and Plans in Windows Azure Pack so that you can offer Self-Service Virtual Machine provisioning.Lessons∙Overview of Windows Azure Pack∙Deploying Windows Azure Pack∙Configuring Windows Azure Pack∙Windows Azure Pack Tenant ExperienceLab : Deploying Windows Azure Pack and provisioning infrastructure servicesAfter completing this module, students will be able to:∙Describe Windows Azure Pack.∙Perform an Express deployment of Windows Azure Pack.∙Configure Windows Azure Pack.Module 12: Azure Site Recovery As with most business critical applications and services, a disaster recovery solution is key to ensure applications and services are available at all times. This is also the case in a virtualized environment. Microsoft Azure Site Recovery provides a service that can be used to orchestrate the replication, failover and recovery of an on-premise Virtual Machine Manager environment to another on-premise Virtual Machine Manager environment such as a disaster recovery site. In addition Azure Site Recovery can be used to replicate the Virtual Machine Manager environment to Azure. In this module you will learn how to configure Azure Site Recovery to provide protection for the Virtual Machine Manager environment including how it can be integrated with Windows Azure Pack to provide disaster recovery for IaaS workloads.Lessons∙Overview of Azure Site Recovery∙Configuring and using Azure Site RecoveryLab : Configuring Azure Site RecoveryAfter completing this module, students will be able to:∙Understand Azure Site Recovery.∙Configure Azure Site Recovery.。
福特网络FortiGate虚拟应用和迁移自动化说明书
1“We needed a solution that would allow us to extend our existing security framework into the cloud, while maintaining full control and visibility across the entire infrastructure. Using the Fortinet Fabric Connector for Azure Cloud Services, together with virtual instances of the FortiGate NGFW, gave us single-pane-of-glass control and visibility over everything.”–Marc Verstraaten, Cloud Architect, Wageningen University & Research CASE STUDYLeading Dutch University for Environmental Research Harnesses the Cloud With FortiGate Virtual Appliances and Migration AutomationWageningen University & Research, located in the town of Wageningen in theNetherlands, is one of the world’s highest-ranking universities in disciplinesspanning environmental science, agriculture, forestry, and ecology.In addition to its renown in education and fundamental research, theestablishment has a strong global position as a supplier of application-orientedand field-based research, collaborating with other educational and researchinstitutes, as well as governments, non-governmental organizations, andbusinesses from around the world.Wageningen University & Research employs over 6,500 staff and currently servesaround 12,500 students from over 100 countries.Securely Harnessing the Potential of Dynamic Cloud ServicesA Fortinet customer since 2014, the university had long leveraged FortiGatenext-generation firewalls (NGFWs) to protect applications and data within theperimeters of its two centrally located data centers.FortiGate NGFWs combine dedicated, purpose-built security processors withthreat-intelligence services from FortiGuard Labs to deliver top-rated security andhigh-performance threat protection.With the addition of FortiManager centralized network management andFortiAnalyzer analytics and automation (collectively known as the FabricManagement Center), network administrators gain powerful network management,automation, and response, with broad visibility and granular device and role-basedadministration across the entire infrastructure.In early 2020, with increasing research collaboration on projects requiring a moreflexible and dynamic infrastructure, the university’s IT team realized that it wouldneed to start moving some of these workloads to the cloud.The team chose Azure Cloud Services from Microsoft as its cloud provider. TheAzure Infrastucture -as-a-Service (I aaS) environment provided the agility, scalability,and control the team needed, but the move to the cloud complicated the process ofmaintaining security. Having witnessed a recent high-profile breach at anotheruniversity in the Netherlands, Wageningen University & Research was takingno chances.“We needed a solution that would allow us to extend our existing securityframework into the cloud, while maintaining full control and visibility across theentire infrastructure,” explains Marc Verstraaten, cloud architect at WageningenUniversity & Research. “Using the Fortinet Fabric Connector for Azure CloudServices, together with virtual instances of the FortiGate NGFW, gave us single-pane-of-glass control and visibility over everything.”Details Customer: Wageningen University & Research Industry: Education Location: The Netherlands Business Impact n n Improved flexibility, scalability, and management of IT resources n n Enhanced security n n Greater control and visibility of global research applications and dataCASE STUDY | Leading Dutch University for Environmental Research Harnesses the Cloud With FortiGate Virtual Appliances and Migration Automation Copyright © 2021 Fortinet, Inc. All rights reserved. Fortinet ®, FortiGate ®, FortiCare ® and FortiGuard ®, and certain other marks are registered trademarks of Fortinet, Inc., and other Fortinet names herein may also be registered and/or common law trademarks of Fortinet. All other product or company names may be trademarks of their respective owners. Performance and other metrics contained herein were attained in internal lab tests under ideal conditions, and actual performance and other results may vary. Network variables, different network environments and other conditions may affect performance results. Nothing herein represents any binding commitment by Fortinet, and Fortinet disclaims all warranties, whether express or implied, except to the extent Fortinet enters a binding written contract, signed by Fortinet’s General Counsel, with a purchaser that expressly warrants that the identified product will perform according to certain expressly-identified performance metrics and, in such event, only the specific performance metrics expressly identified in such binding written contract shall be binding on Fortinet. For absolute clarity, any such warranty will be limited to performance in the same ideal conditions as in Fortinet’s internal lab tests. Fortinet disclaims in full any covenants, representations, and guarantees pursuant hereto, whether express or implied. Fortinet reserves the right to change, modify, transfer, or otherwise revise this publication without notice, and the most current version of the publication shall be applicable.May 5, 2021 3:46 AMD:\Fortinet\2021 Rebranded templates\Case Studies\May\WUR\cs-leading-dutch-university-V1-552021\cs-leading-dutch-university-V1-5520211007903-0-0-EN Solutions n n FortiGate VM n n FortiGate n n FortiManager n n FortiAnalyzer The Fortinet Security Fabric is an architectural approach that enables themultiple security elements of a network to act together as a single, intelligent,responsive entity.Since FortiGate virtual appliances are built on the same FortiOS operatingsystem as their physical counterparts, they enable customers to create theoptimal architecture for their specific environment, balancing the unparalleledperformance of the physical form factor with the flexibility and scalability of thevirtual, to provide seamless visibility and control from the network core right out tothe edge.Through FortiManager and the Fortinet Security Fabric, configuration andpolicy management can then be consolidated across both physical and virtualenvironments through a single pane of glass, simplifying management andreducing the potential for service degradation or bottlenecks.Non-Fortinet components, such as those within the Azure Cloud Servicesenvironment, can then be brought under the protective umbrella of the FortinetSecurity Fabric through prebuilt application programming interfaces (APIs) knownas Fabric Connectors.For complex application development operations such as those of WageningenUniversity & Research, one of the key risks associated with moving workloadsinto the cloud is the potential introduction of vulnerabilities resulting fromconfiguration errors and manual data compilation.“The ability to integrate automated cloud deployment scripts into the already-familiar management interface of FortiManager and FortiAnalyzer was anotherkey advantage for us,” adds Verstraaten. “Our team was well-versed in theimplementation of on-premises security policies but lacked experience with thecloud environment.”Ready for the Future“The ability to integrate automated cloud deployment scripts into the already-familiar management interface of FortiManager and FortiAnalyzer was another key advantage for us. Our team was well-versed in the implementation of on-premises security policies but lacked experience with the cloud environment.”– Marc Verstraaten, Cloud Architect, Wageningen University & ResearchHaving completed the first phase of their new cloud migration, the university is now looking to optimize service delivery through the built-in load-balancing capabilities of the FortiGate virtual appliances.“One of the things we particularly like about the Fortinet solution is the range of functionality you get right out of the box,” comments Verstraaten. “It means we can move at a pace that suits us, deploying additional capabilities as and when we need them.”The university’s stated mission, “To explore the potential of nature to improve the quality of life” is undoubtedly one ofincreasing significance in the face of globally accelerating technological and environmental change. Through the FortinetSecurity Fabric and the continued efforts of Marc Verstraaten and his team, Wageningen University & Research is now able to pursue that mission with a greatly reduced risk of disruption from the ever-evolving specter of cyberattack.。
Studio 5000 Logix Designer Level 4 Integrated Moti
Course NumberCCN144Course PurposeAfter completing this course, you should be able to configure, test, tune, and program CIP motion axes in the Studio 5000 Logix Designer® programming environment.COURSE AGENDADAY 1• Creating a Studio 5000 Logix Designer Project for Integrated Motion on an EtherNet/IP Network • Adding Drives and Configuring Axes for Integrated Motion on an EtherNet/IP Network• Testing Hardware for Integrated Motion on anEtherNet/IP NetworkControlLogix/Studio 5000 Studio 5000 Logix Designer Level 4:Kinetix 6500 (CIP) ProgrammingWHO SHOULD ATTENDIndividuals who need to configure and programLogix5000 motion control systems should attend this course. In addition, only students who are already familiar with Logix5000 systems and general motion control should attend this course.PREREQUISITESTo successfully complete this course, the following prerequisites are required:• Completion of the Motion Control Fundamentalscourse (Course No. CCN130) or equivalent knowledge of Kinetix® 6500 drives, feedback devices, and servo motion systems• Completion of the Studio 5000 Logix Designer Level 3: Project Development course (Course No. CCP143)or equivalent experience.STUDENT MATERIALSTo enhance and facilitate the students’ learningexperiences, the following materials are provided as part of the course package:• Student Manual– Includes the key concepts, definitions, examples, and activities presented in this course • Lab Book–Provides learning activities and hands-on practice. Solutions are included after each exercise for immediate feedback.• Studio 5000 Logix Designer and Logix5000 Motion Control Procedures Guide–Provides the steps required to complete common motion-related tasks within a Logix Designerproject, as well as basic project organization tasks.HANDS-ON PRACTICEThroughout this course, you will have the opportunity to practice the skills you have learned through a variety of hands-on exercises using ABT-TDK6500EN2TR and ABT-TDCLX3 workstations. Exercises focus on the skills introduced in each lesson.You will use the Kinetix and ControlLogix® workstations, containing real and simulated devices, to practice the tasks involved in programming a motion controlapplication. After configuring a project that contains the required hardware, you will program a variety of motion routines and motion instructions commonly used in integrated motion applications. Finally, you will begin to employ dependent motion in the form of gearing and camming instructions.NEXT LEARNING LEVELOnce you have an understanding of the topics and skills covered in this course, you may want to attend specific motion training such as:• Studio 5000 Logix Designer Level 5: Advanced Motion Programming course (CCN190).COURSE LENGTHThis is a four-day course.Allen-Bradley, ControlLogix, Kinetix, Logix5000, and Studio 5000 Logix Designer are trademarks of Rockwell Automation, Inc.Trademarks not belonging to Rockwell Automation are property of their respective companies.Publication GMST10-PP558E-EN – January 2020 | Supersedes Publication GMST10-PP558D-EN - March 2018Copyright © 2020 Rockwell Automation, Inc. All Rights Reserved. Printed in USA.To be respectful of the environment, Rockwell Automation is transitioning some of its training courses to a paperless format. Students are asked tocomplete downloads and bring personal devices to these classes. A full list of digital/paperless courses is currently available through your local distributor.。
Silicon Labs Simplicity Studio 5 用户指南说明书
Tech Talks LIVE Schedule –Presentation will begin shortlyFind Past Recorded Sessions at: https:///support/trainingFill out the survey for a chance to wina BG22Thunderboard!TopicDateBuilding a Proper Mesh Test Environment: How This Was Solved in Boston Thursday, July 2Come to your Senses with our Magnetic Sensor Thursday, July 9Exploring features of the BLE Security Manager Thursday, July 23New Bluetooth Mesh Light & Sensor Models Thursday, July 30Simplicity Studio v5 IntroductionThursday, August 6Long Range Connectivity using Proprietary RF Solution Thursday, August 13Wake Bluetooth from Deep Sleep using an RF SignalThursday, August 20Silicon Labs LIVE:Wireless Connectivity Tech Talks Summer SeriesWELCOMESilicon Labs LIVE:Wireless Connectivity Tech TalksSummer SeriesIntroduction to Simplicity Studio 5August 6th, 2020https:///products/development-tools/software/simplicity-studio/simplicity-studio-5What is Simplicity Studio 5?§Free Eclipse Based Development Environment§Designed to support Silicon Labs IoTPortfolio§Provides Access to Target Device-SpecifiedWeb & SDK Resources§Software & Hardware Configuration Tools§Integrated Development Environment (IDE)§Eclipse based, C/C++ IDE§GNU ARM Toolchain§Advanced Value Add Tools§Network Analysis, Code Correlated EnergyProfiling, Configuration Tools, etc.The Data Driving Simplicity Studio 5?Simplicity StudioGecko SDK Dev Guides, TutorialsAPI RMsRef Manuals,Datasheets, ErrataStacks, Gecko Platform,Examples, Demos,metadataHardware KitBoard IDSimplicity Studio 5 -LauncherOn the Welcome PageYou Can•Select Target Device •Start a New Project •Access Support Resources and EducationalPressing ‘Welcome’ onthe tool bar will return to Welcome page at any time.1. Welcome & Target SelectionThis is a “get started” section to help with device or board selection12342. Debug Adapters Area shows connected debug adapters including Silicon Lab kits, Segger, J-Link, etc…3. My ProductsEditable list of products you may wish to use as target devices 4. MenuMenu & Tool bar provide access to a number of functions and shortcutsLauncher Perspective -Overview1. General InformationGI card shows debugger,debugger mode, firmwareversions for adapter andsecurity, SDK12 342. Recommended QSGs Quick links to recommended quick start guides for selected product.3. BoardBoard shows which evaluation board is being used and provides easy access to its documentation.4. Target PartTarget part shows full part number and also provides easy access to its documentationLauncher Perspective –Example Projects1. Technology Filter Keyword Filter box and Technology Type check boxes let you dial into the example you are looking for.122. Resource ListResource list will show corresponding example projects that are intended for your selectedtechnology and target device.Launcher Perspective –Documentation1. Resource FilterKeyword Filter box andResource Type checkboxes let you dial into theresource you are lookingfor (Data Sheet, App Note,Errata, QSG, etc…).1232. Technology TypeTechnology check boxesnarrow your search basedon a give technology(Bluetooth, Bootloaders,Thread, Zigbee, etc…).3. ResourcesList of resources that willnarrow as you selectfilters (Data Sheet, AppNote, Errata, QSG, etc…).Launcher Perspective –Demos1. Demo FilterDemo Filter allows you to narrow your search of demos for your selected device.122. DemosList of Pre-compiled demos that are ready to be programmed into your selected device.Launcher Perspective –Compatible Tools1. Compatible ToolsLaunching pad for toolssuch as Hardware1Configurator, NetworkAnalyzer, Device Console,Energy Profiler, etc…Launcher Perspective –Tools –Pin Configuration ToolPin Configuration ToolSimplicity Studio 5 offers aPin Configuration Tool thatallows the user to easilyconfigure new peripheralsor change the propertiesof existing ones. Thegraphical view will differbased on the chip beingused.Simplicity Studio 5 -IDEIDE –Overview1. Tool Bar & MenuLaunching pad for tools 123452. Project ExplorerDirectory structure and allfiles associate with theproject.4. Editor & ConfiguratorsCode editing windows andconfigurators forproject/technologies.3. Debug AdaptersShows connecteddebuggers and EVKs5. Additional WindowsProblems, Search, CallHierarchy, ConsoleIDE –Project Configurator Overview1. Target & SDKAllows user to changedevelopment target andSDK.1232. Project DetailsCan change import mode& force generation.3. Project GeneratorsAllows user to modifywhat files are beinggenerated by projec tIDE –Project Configurator Software Components1. ComponentExpand components to see categories and sub-categories.1232. Selected Component View details of a given component. Gearindicates a configurable component. Check marks show installed components.3. Filters & KeywordsHelp you to search various component categoriesIDE –Configurators (GATT)1. GATT Configurator View, Add, Remove GATT Profiles, Services, Characteristics, and Descriptors122. GATT EditorAllows user to view & modify settings in the Profiles, Services, Characteristics and Descriptors within the GATT.IDE –Configurators (Editing the GATT)EDIT (Device Name)From GATT Configuratorclicking on an editableitem such as device namewill open up a newwindow allowing the userto see content that can beedited and several optionsfor that content that canbe selected/de-selected.Simplicity Studio 5 -MigrationSimplicity Studio -Developer Options*Bugfixes provided per software longevity commitment (https:///products/software/longevity-commitment )Developer ProjectExisting ProjectNew Project GSDK v2.7.x *Simplicity Studio 4*GSDK v3.x.x Simplicity Studio 5Secure VaultFinal Developer BinariesSS4/GSDK2.7x Continuance Option *MigrationToolkitProcess and availability varies by technologyGSDK v2.7.x *Simplicity Studio 4*SS5/GSDK3.x Upgrade OptionLIMITED SUPPORTSubject to longevity commitmentGS D K 2.x t o 3.x = M aj o r C h a n g e .Simplicity Studio –Project StructureBluetooth SDK v2.x Project StructureBluetooth SDK v3.x Project StructureProject Structure There is a change in project structure from GSDK v2.x to GSDK v3.x.It’s now much easier to see which file can be modified by the generator and it’s easier to find/identify the configuration files This is important because withGSDKv3.x many more files are generated by the addition of software components.Simplicity Studio –BGAPI CommandsBGAPI CommandBGAPI CommandsBGAPI Commandschange both theirname and theirstructure to make theerror checking andhandling of returnvalues simpler.Simplicity Studio –Changes to BGAPI CommandsChanges to BGAPI Commands With manycommands, renaming means only changing gecko_cmd_ to sl_bt_.Other functions have been renamed due to changes infunctionality, changed API class, or simply to make the functions more logical.Some API functions have been split into multiple ones while others have been merged.123Simplicity Studio 5 -DemoSimplicity Studio 5 -LinksSimplicity Studio –Useful LinksSimplicity Studio 5https:///products/development-tools/software/simplicity-studio/simplicity-studio-5 Simplicity Studio 5 User Guidehttps:///simplicity-studio-5-users-guide/latest/indexQuick Start Guide Bluetooth SDK v3.xhttps:///documents/public/quick-start-guides/qsg169-bluetooth-sdk-v3x-quick-start-guide.pdfTransitioning from Bluetooth SDK v2.x to v3.xhttps:///documents/public/application-notes/an1255-transitioning-from-bluetooth-sdk-v2-to-v3.pdfBluetooth SDK 3.0.0.2 Release Noteshttps:///documents/public/release-notes/bt-software-release-notes-3.0.0.2.pdfThe Largest Smart Home Developer EventS E P T E M B E R9 –1 0, 2 0 2 0Immerse yourself in two days of technical training designedespecially for engineers, developers and product managers.Learn how to"Work With" ecosystems including Amazon and Google and join hands-on classes on how tobuild door locks, sensors, LED bulbs and more.Don't miss out, register today!w o r k s w i t h.s i l a b s.c o mThank you…..Questions? 。
NetSupport School名称和连接服务器技术文档说明书
OverviewA NetSupport Name & Connectivity Server (also known as the ‘Gateway’ in older versions) is provided as an optional component within the NetSupport School Windows installation setup.exe file.Its purpose is to provide a simple and reliable method of locating and connecting a NetSupport School Tutor to NetSupport School Student devices in LAN/wireless network environments.Benefits of implementing a Name & Connectivity Server (NCS):• Resolves connectivity issues.• Negates the need to browse the network to locate Student devices.• Reduces the time to locate the Student devices.• Reduces the need to configure and maintain browsing broadcast or IP address range entries in the Tutor/Tech Console configuration.• The Tutor/Tech Console uses the current IP address registered by the Student device on the NCS when establishing connections, resolving potential DNS resolution issues.Scenarios where a Name & Connectivity Server can be used to resolve connection issues are:• Large networks where Student devices may not respond in time to be located using the standard NetSupport School browse function.• Networks with UDP restrictions making it impossible to perform a UDP browse to locate Student devices.• Networks with multiple subnets or VLANs where maintaining the Tutor/Tech Console browse settings becomes an issue.How it worksOnce implemented, the Student devices on start-up will register the machine name, IP address, MAC address, room name and logged on user name with the Name & Connectivity Server.In turn, the Tutor/Tech Console will request the Student device details from the Name & Connectivity Server instead of performing a browse across the network to find the Student devices to connect to.Installing a Name & Connectivity ServerThe Name & Connectivity Server (NCS) is designed to be installed and run on a physical or virtual machine running a Windows Server operating system. The server must be accessible from all Tutor/Tech Console and Student devices on the network.The server the NCS is installed on should have a static IP address to prevent any server connection issues within a DHCP environment.The NCS can be installed as a standalone component (or along with other NetSupport School components). In the Custom Setup screen, select the Name & Connectivity Server option.At the end of the installation, the Name & Connectivity Server configuration window will launch.Configuring the Name & Connectivity ServerGeneral tabListen on all IP interfacesBy default, the installed Connectivity Server component uses the local IP address(es) and communicates using port 443 - this can be changed if required.Note: On a machine that also has the Web Server (IIS) Role added, the Connectivity Server port will need to be changed to the NetSupport registered port 3085.Listen on specified IP interfacesIf the Connectivity Server machine has multiple network cards installed, you can add the specific IP address to use. Click Add and enter the required IP address.CMPI (Communications Management Packet Interval)When configured for Connectivity Server connections, the Student device confirms its availability by periodically polling the Connectivity Server. By default, a network packet is sent every 60 seconds, but you can change this if required.Note: Once the Connectivity Server is active, the CMPI value also controls how long it takes for the Student devices to be removed from the list of available machines shown on the Clients tab of the ‘Connectivity Server Console’ after it is no longer available on the network.Event log filesConnectivity Server activity during an active session is recorded in a text file with a default file name of GWxxx.log. Log files can be useful for checking which Student devices and Tutor/Tech Consoles have connected to the Connectivity Server.Location:By default, the GWxxx.log files are stored in the C:\Program Files (x86)\Common Files\NSL\Connectivity Server folder. The Browse button can be used to specify an alternative folder path.Max file size (KB):To limit the size of the log file, you can specify a maximum file size. When the limit is reached, a new file will be created, for example GW001.log, GW002.log, GW003.log etc.Note: For changes to the log file settings to take affect the Gateway32 service on the machine will need to be restarted. Keys tabAccess to a Connectivity Server is protected by use of a Connectivity Server key (security key). The Connectivity Server will not accept connections from a NetSupport School Tutor, Tech Console or Student device unless the matching key is also configured on those devices.The key is therefore a pre-requisite when configuring the Name & Connectivity Server settings.1. Click Add and enter a suitable description for the key.2. Click Set and enter your chosen Connectivity Server key. An encrypted value will be displayed. For accuracy,enter the key twice. The key must be between 8 and 15 characters and can contain upper and lower case letters, numbers and/or special characters. Click OK. (It may help to make a note of the key). As mentioned, the same key must also be used when configuring both the NetSupport School Tutor and Student devices to communicate with the Server.3. The key description and creation date will now be displayed in the Connectivity Server Keys list.Licenses tabThe Licenses tab will show the details of the NetSupport School licence(s) that have been applied to the Name & Connectivity Server.When the Name & Connectivity Server starts on the machine, it will attempt to automatically activate the NetSupport School licence over the internet. If the automatic activation fails for any reason, the licence can be manually activated using the Activate button.Multiple NetSupport School licences can be applied to the Name & Connectivity Server by renaming the additional NSM.LIC files and placing them in the NetSupport School installation directory: C:\Program Files (x86)\NetSupport\ NetSupport School.The NSM.LIC files will need to be renamed sequentially so that they are read by the Name & Connectivity Server – for example NSM.LIC, NSM-1.LIC, NSM-2.LIC, NSM-3.LIC etc.All applied NetSupport School licence files will be listed on the Licenses tab and will require activating on the Name & Connectivity Server.Security tabNo changes are required to be made on the Security tab.Classes tabNote: This option is only applicable if you intend to use NetSupport’s SIS/MIS (Student/Management Information System) connection mode.From version 12.60, NetSupport School allows you to import school timetable/roster data directly from a SIS/ MIS system. This can be done by either linking directly to the SIS/MIS data using ClassLink OneRoster/Google Classroom or by importing the data from a zip file which has been extracted from the SIS/MIS.The NetSupport Name & Connectivity Server can be configured to provide direct integration with ClassLink OneRoster or Google Classroom, allowing access to the school’s SIS classrooms and student accounts.Class datasourceClassLink OneRosterSelect this option to link to ClassLink OneRoster and enter your school/district’s Tenant ID. For added validation, enter the matching NetSupport School Security/API Key provided in your Roster Server Portal. (The key can be found in the OneRoster Server Portal by selecting, Applications > NetSupport School > API Key and Secret.) OneRoster zipped CSV filesAlternatively, you can import zipped CSV files. Click and browse to the required files.Notes:• The CSV files must conform to the OneRoster standard.• The first time you configure the Connectivity Server to use OneRoster zip files, you must do a bulk export of your SIS data.Google ClassroomSelect this option to link to Google Classroom. Click and browse to one of the JSON files that you have copied to the machine (both files must be present, but either file can be selected). Click here for information on how to set up a Google Classroom Project and create the required JSON files.Click Apply and you will be prompted to sign into Google G Suite with administrator credentials and grant access to NetSupport School.Syncs per dayBy default, the Connectivity Server will sync with OneRoster or Google Classroom once a day. This can be amended by entering a different value here, you can also specify the time the sync will take place.Click Sync now to synchronise the Connectivity Server. Clicking Reset Data will clear all previous data stored and if there is a lot of data to download, may take some time.Once you are happy that the Name & Connectivity Server is configured as required, click OK.The NetSupport Connectivity Server Console icon should now appear in the system tray. If any of the settings need editing, right-click on the icon and select Configure Connectivity Server. Double-click the icon to open the Connectivity Server Console window.This details the activity during a NetSupport School-managed lesson, i.e. Tutor and Student devices communicating through the Server.You can now configure the required NetSupport School components to communicate with the NCS.Configuring the NetSupport School Windows Tutor and Tech Console to use the NCSThe following instructions assume that the Tutor and Tech Console components are already installed on the required Windows machines.1. Open the NetSupport School Tutor or Tech Console.2. To configure the Tutor, select {School}{Configuration - Network and Wireless Settings} from the drop-downmenu.OrTo configure the Tech Console, select {Admin}{Configuration - Network and Wireless Settings} from the drop-down menu.3. Select Use Name & Connectivity Server and click Settings. Enter the IP address of the machine wherethe Name & Connectivity Server was installed, the port number (if different from the default 443 port), and click Set to enter the matching security key entered when installing the Server. An encrypted value will be displayed.Note: When Use Name & Connectivity Server is enabled please ensure Connect By Hostname (DHCP/WINS) is disabled to avoid conflicts.4. Click OK to apply the settings.By default, the Tutor and Tech Console settings are stored in the registry for each logged-on user in the following locations:HKEY_CURRENT_USER\Software\NetSupport Ltd\PCICTL\ConfigList\NetSupport SchoolHKEY_CURRENT_USER\Software\NetSupport Ltd\PCICTL\ConfigList\NetSupport Tech ConsoleConfiguring NetSupport School Student devices to use the NCSThe following instructions explain how to configure the NetSupport School Student for Windows, and assumes the Student component is already installed on the devices.1. In the NetSupport School program group, select the NetSupport School Student Configurator.OrLaunch the Student Configurator exe file, PCICFGUI, from the NetSupport School Program Files (x86) folder.2. In Network Settings, select Use Name & Connectivity Server and click Configure. Enter the IP address of themachine where the Name & Connectivity Server is installed, the port number (if different from the default 443 port), and click Set to enter the matching security key entered when installing the Server. An encrypted value will be displayed. Click OK.3. Click OK to apply the settings. Student devices will need to be restarted for the changes to take effect.By default, the Student component settings are stored in the client32u.ini file located in the following folder:C:\Program Files (x86)\NetSupport\NetSupport SchoolApplying the NCS settings to NetSupport School components by Group PolicyThe Name & Connectivity Server settings can also be applied to the Tutor, Tech Console and Student Configurations via Group Policy.Machine and user-based NetSupport School ADMX/ADML files are provided with the software and are available on a machine that has the NetSupport School Tutor installed – in the folder:C:\Program Files (x86)\NetSupport\NetSupport School\ADM Templates\ADMXApplying the Name & Connectivity Server settings to the components using the machine-based ADMX files via a Group Policy will ensure that the settings will be the same for all users that log on to the Tutor and Student devices. Additional information for creating and applying the NetSupport School configuration via a Group Policy can be found in the following Technical Document:Configuring the NetSupport Connectivity Server details for NetSupport School Students and Tutors by Group Policy (GPO)Note: When applying the NCS security key using a Group Policy, you must enter the encrypted value that was returned when originally configuring the Tutor, Tech Console and Student components rather than the plain text version. The encrypted value is different for each component so care must be taken to ensure that the correct encrypted value is used in the Group Policies, as shown in the example below.Troubleshooting connectivity issuesThe main cause of connection issues between the Name & Connectivity Server and NetSupport School components are that the IP address, port number and Connectivity Server key do not match on all devices. This should be checked first by re-entering the details and restarting the Name & Connectivity Server process and Student components.If the above does not resolve the connectivity issues, investigate the following:1. Is the Name & Connectivity Server running?In normal operation, a NetSupport Connectivity Server Console icon will be displayed in the system tray on the machine the Name & Connectivity Server is installed on.The Name & Connectivity Server runs as an Automatic Startup Type service called Gateway32, so check that this service is running.2. Is the Name & Connectivity Server listening?A GWxxx.log file is created on the Name & Connectivity Server that records the Gateway32 service start up andthe machine names of the Tutor/Tech Console and Student devices connecting to the server.The GWxxx.log files are located in the C:\Program Files (x86)\Common Files\NSL\Connectivity Server folder by default.If an entry such as “Failed to bind to listening port...” is seen in the log file, this is usually indicative that another application is already using the port number configured for the Name & Connectivity Server. As the default port for the Name & Connectivity Server is 443, this will normally be the Web Server (IIS) role that may also be installed on the server.Running “netstat –an | more” from a command prompt will list the ports in use on the server.If a port conflict is the issue, either reconfigure the Name & Connectivity Server to use an alternative port number or reconfigure/disable the application that is causing the conflict.3. Are the Students connected to the Name & Connectivity Server?There are two ways to confirm if the Tutor/Tech Console and Student devices are connected to the Name & Connectivity Server.The first is to right-click the NetSupport Connectivity Server Console icon in the system tray on the server and select Open. This will open the Console window.Selecting the Clients tab will display the information for all of the devices that are currently connected to the Name & Connectivity Server.The second is to use the entries in the GWxxx.log file to see the machine names of the devices that have connected to the server.4. Has the maximum number of device connections been reached?The Name & Connectivity Server restricts the number of devices that are able to concurrently connect to the server based on the number of Clients in the NSM.LIC file applied on the server.A ‘License Limit Exceeded’ entry will be seen in the GWxxx.log file if more devices than are allowed in theNSM.LIC licence file attempt to connect to the Name & Connectivity Server.5. Is the student service running?With a default configuration applied, a NetSupport School Client icon will be seen in the system tray on the Student devices.The Student component runs as an automatic startup type service called Client32, so check that this service is running on the device.6. Is the Name & Connectivity Server visible to the Tutor/Tech Console and Student devices?If no Tutor/Tech Console or Student devices are seen on the Clients tab on the Name & Connectivity Server, it is possible to use two connection tests from the devices to confirm that the NCS is visible on the network:Using the Name and Connectivity Server test pageOn the Tutor/Tech Console or Student device, open a browser and enter the following URL:http://[ServerIPAddress]:[PortNo]/testpage.htm(Where [ServerIPAddress] is the IP address of the Name & Connectivity Server, and [PortNo] is the port number that it is configured to use.)If the device can successfully reach the Name & Connectivity Server test page, a NetSupport Gateway Server page will be displayed in the browser.Using the Telnet connection testNote:if the Telnet Client is not installed on the device, it can be enabled in the Control Panel | Programs and Features | Turn Windows features on or off section.On the Tutor/Tech Console or Student device , open a command prompt and enter in the following command and press Enter:telnet [ServerIPAddress] [PortNo](Where [ServerIPAddress] is the IP address of the Name & Connectivity Server, and [PortNo] is the port number that it is configured to use.)If the device can successfully connect to the Name & Connectivity Server port, the command prompt window will go blank.If either of these two tests fails, the device is being prevented from connecting to the Name & ConnectivityServer on the network. This is usually due to firewall/endpoint or restrictions on the device or the network.。
jupyterlab 建议重新编译
JupyterLab是一个非常实用的工具,为了更好地利用它,我们建议重新编译JupyterLab。
下面是一些建议:1.了解JupyterLab的需求和功能在重新编译JupyterLab之前,首先需要了解JupyterLab的需求和功能。
JupyterLab是一个交互式的开发环境,可以用于数据科学、机器学习等领域的开发和编程。
它具有丰富的功能和插件,可以帮助用户更好地进行数据分析和可视化。
2.查看最新的JupyterLab版本在重新编译JupyterLab之前,需要查看最新的JupyterLab版本。
新版本通常会修复一些bug和优化一些功能,因此可以提高JupyterLab 的稳定性和性能。
3.备份已有的数据和配置文件在重新编译JupyterLab之前,建议备份已有的数据和配置文件。
这样可以避免因重新编译导致数据丢失或配置文件损坏的情况。
4.使用合适的编译工具在重新编译JupyterLab时,需要选择合适的编译工具。
通常可以使用pip、conda等包管理工具进行编译。
确保选择合适的编译工具可以提高编译的效率和成功率。
5.按照冠方文档进行编译在重新编译JupyterLab时,建议按照冠方文档进行编译。
冠方文档通常会提供详细的编译步骤和注意事项,可以帮助用户顺利完成编译。
6.测试编译结果在重新编译JupyterLab完成后,建议测试编译结果。
可以运行一些简单的代码,或者使用一些常用的插件进行测试,确保重新编译的JupyterLab能够正常运行。
7.定期更新JupyterLab在重新编译JupyterLab之后,建议定期更新JupyterLab。
新版本通常会提供一些新功能和优化,可以提高JupyterLab的易用性和性能。
通过以上建议,希望可以帮助大家更好地重新编译JupyterLab,提高JupyterLab的稳定性和性能,为数据科学和机器学习等领域的开发和编程提供更好的支持。
JupyterLab是一个非常实用的工具,尤其是对于数据科学家、研究人员和开发人员来说,它提供了一个交互式的开发环境,使他们能够轻松地进行数据分析、可视化、编程和协作。
Jalor5学习心得
Jalor5学习⼼得jalor5是⼀套功能强⼤的框架,该框架集成了spring、mybatis、cxf、⽇志、异常等组件,和其它未提及的部分组件,如消息组件。
它还⾃带了权限管理,内容管理,国际化等功能,该框架在项⽬开发中起到了缩短项⽬周期和降低技术难度的功能。
虽然jalor5的开发和使⽤都有⼤量⽂档,但是我觉得⼀个从未接触过jalor5甚⾄未接触过Spring框架的⼈来说,单单靠jalor5的⽂档,还是会遇到很多的问题,⽐如环境搭建,数据流的⽅向等。
jalor5是在jdk1.5的环境下开发的,⽂档中说使⽤其它的jdk版本或许会存在问题,但是如果⽤jdk1.5的话jalor5会有错误,⽽且在新建项⽬中也存在⼀些细节与⽂档上的差别,⽂档中的部分例⼦不够全⾯,使得在学习过程中遇到了很多⼩的但是⼜难以解决的问题。
就算环境搭建都没问题了,在建好项⽬了之后也会遇到个别的问题,⽐如数据库的初始化、包名的固定命名、请求url的写法,各个应⽤层代表的意思,接⼝层中path注解的命名都会遇到各种不同的问题。
下⾯我说说我遇到的问题和解决⽅法:问题(仅是我遇到的,⼤家使⽤的时候不⼀定会遇到):1、lib包的编译问题,刚开始的时候提⽰编译版本不对,要使⽤jdk1.5版本编译。
解决⽅法:按照提⽰改成jdk1.5编译即可(改了之后再切换回jdk1.6错误不提⽰了,很是奇怪)。
2、数据库初始化问题。
jalor5的使⽤必须初始化数据库,⽽oracle的安装和导⼊数据脚本是必要的,但是导⼊脚本过程中也要注意修改脚本中的appname和scope的名字,我是只改了appname为⾃⼰的应⽤程序名即可。
因为jalor5的后台管理菜单的内容是根据appname去获取的,所以这⼀步是必须的。
3、新建项⽬中包的命名,我⾃⼰随便命名,结果程序⼀直报错。
解决⽅法很简单,修改包名为com.huawei.it打头即可,当然相应的层命名必须包含有dao、service等全⼩写的单词,因为spring配置是要去扫描这些配置的路径的。
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Generating a JavaServer Faces CRUD Application from aDatabaseIn this tutorial, you use the NetBeans IDE to generate and deploy a web application that displays data from a database. In the tutorial you generate a web application with CRUD (Create, Read, Update, Delete) functionality that uses the Java Persistence API (JPA) to manage the database transaction. In the tutorial you first create entity classes based on tables in a database and then create JavaServer Faces (JSF) pages from the entity classes using the JSF Pages from Entity Classes wizard.The JSF Pages from Entity Classes wizard enables you to quickly and easily generate JSF pages for working with a database based on the entity classes in your project. The wizard was updated in NetBeans IDE 6.1 to add new features, support more cases and to generate more robust code. The improvements to the wizard include the following:∙The generated code is simpler and easier to maintain and customize.∙Correct handling of all relationship types, generated and non-generated IDs, and embedded IDs, including embedded ID fields that correspond to foreign key columns.∙Required checks prevent non-nullable column violations.∙Orphan checks prevent non-nullable column violations in related entities.The generated code also includes checks to verify that the current entity is correct in order to prevent errors if a user deviates from the normal page flow. Errors may occur, for example, when working in multiple browser tabs.Using the code generated by the wizard, it is now easier to remove fields from the generated JSPs.It is also now much easier to apply styles to the generated pages.Contents∙Creating the Databaseo Viewing the Database Structure∙Creating the Web Application Project∙Generating the Entity Classes from the Database∙Editing the Entity Classes∙Generating JSF Pages From Entity Classes∙Running the ProjectTo follow this tutorial, you need the following software and resources.Software or Resource Version RequiredNetBeans IDE Web & Java EE version 6.1Java Developer Kit (JDK) Version 6 or version 5GlassFish Application Server V2Consulting Agency Database DownloadCreating the DatabaseThis tutorial uses a consulting agency database called consult. The database is not included when you install the IDE so you need to first create the database to follow this tutorial.The consult database was designed to demonstrate the scope of IDE support for handling a variety of database structures. The database is thus not intended as an example of recommended database design. Instead, it attempts to incorporate many of the relevant features that are potentially found in a database design. For example, the consult database contains all possible relationship types, composite primary keys, and many different data types. See the tables for a more detailed overview of the database structure.1.Download the create-consult.sql file and save the file to your local system.2.In the Services window, expand the Databases node, right-click the JavaDB node andchoose Start Server.3.Right-click the JavaDB node and choose Create Database.4.Type consult as the Database Name, User Name, and Password in the Create Java DBdialog. Click OK.A new node appears under the Databases node(jdbc:derby://localhost:1527/consult [consult on CONSULT]).5.Right-click the new node and choose Connect.6.Expand the consult database node, right-click the Tables node, and choose ExecuteCommand to open the SQL editor.7.Copy and paste the contents of the create-consult.sql file into the SQL editor.Note: Make sure that the consult database is selected in the Connection drop-down list.8.Click the Run SQL button in the SQL toolbar.When you click Run SQL, the following output appears in the Output window.Viewing the Database StructureTo see the if the tables were created correctly, right-click the Tables node in the Services window and choose Refresh. You should see the database tables under the Tables node. You can expand atable node to see the table columns, indexes and any foreign keys. You can right-click a column and choose Properties to view additional information about the column.If you look at the structure of the consult database you can see that the database contains tables that have a variety of relationships and various field types. When creating entity classes from a database, the IDE automatically generates the appropriate code for the various field types. The following table describes the tables found in the consult database.Database Table Description Design FeaturesCLIENT A client of the consulting agency Non-generated, composite primary key (whose fields do not constitute a foreign key)CONSULTANT An employee of the consultingagency whom clients can hire on aIncludes a resume field of type LONGcontract basis VARCHARCONSULTANT_STATUS A consultant's status with theconsulting agency (for example,Active and Inactive are possiblestatuses)Non-generated primary key of typeCHARRECRUITER An employee of the consulting agency responsible for connecting clients and consultantsPROJECT A project that a client staffs withconsultants of the consultingagencyNon-generated, composite primarykey that includes two fieldsconstituting a foreign key to theCLIENT tableBILLABLE A set of hours worked by aconsultant on a project, for whichthe consulting agency bills therelevant clientIncludes an artifact field of type CLOBADDRESS A client's billing addressPROJECT_CONSULTANT Join table indicating whichconsultants are currentlyassigned to which projectsCross-references PROJECT andCONSULTANT, the former having acomposite primary keyThe consult database includes a variety of relationships. When creating entity classes from a database, the IDE automatically generates the appropriate code for the table relationships, adding the appropriate annotations to the fields in the entity classes. The following table describes the table relationships found in the consult database. (The inverse relationships are not shown.)Database Table RelatedTableRelationship InformationCLIENT RECRUITER nullable one-to-one with manual editing; nullable one-to-many if not editedCLIENT ADDRESS non-nullable one-to-oneCLIENT PROJECT non-nullable one-to-many; in a Project entity, the value of the client field is part of the Project's primary keyCONSULTANT PROJECT many-to-manyCONSULTANT BILLABLE non-nullable one-to-manyCONSULTANT_STATUS CONSULTANT non-nullable one-to-manyRECRUITER CONSULTANT nullable one-to-manyPROJECT BILLABLE non-nullable one-to-manyNow that the database is created, you can create the web application and use the Entity Classes from Database wizard to generate entity classes based on the database tables.Creating the Web Application ProjectIn this exercise you create a web project and add the JavaServer Faces framework to the project. When you create the project, you will select JavaServer Faces in the Frameworks panel of the New Project wizard.1.Choose File > New Project (Ctrl-Shift-N).2.Select Web Application from the Web category and click Next.3.Type ConsultingAgency for the project name and set the project location.4.Deselect the Use Dedicated Folder option, if selected.For this tutorial there is little reason to copy project libraries to a dedicated folder because you will not need to share libraries with other users.Click Next.5.Set the server to GlassFish and set the Java EE Version to Java EE 5. Click Next.6.Select the JavaServer Faces checkbox in the Frameworks panel. Click Finish.Note: When selecting the framework, be sure to select the JavaServer Faces framework, NOT Visual Web JavaServer Faces.When you click Finish, the IDE creates the web application project and opens WelcomeJSF.jsp in the editor. You can close WelcomeJSF.jsp because you will not need to edit this file. Generating the Entity Classes from the DatabaseAfter you create the database and the database connection is registered with the IDE, you can use the Entity Classes from Database wizard to quickly generate entity classes based on the tables in the database. The IDE can generate entity classes for each table that you select, and can also generate any necessary entity classes for related tables.1.In the Projects window, right-click the ConsultingAgency node, and choose New > EntityClasses from Database.2.Select New Data Source from the Data Source drop-down list to open the Create DataSource dialog.3.Type jdbc/consult as the JNDI Name and selectjdbc:derby://localhost:1527/consult [consult on CONSULT] as the Database Connection. Click OK to close the dialog box and return to the wizard.The tables in the consult database appear in the Available Tables listbox.4.Click Add All in the wizard. Click Next.5.Type entities as the Package. Make sure that the checkbox to generate named queries isselected.6.Click Create Persistence Unit to open the Create Persistence Unit dialog box. Click Createin the dialog box to create the persistence unit and return to the wizard.You can keep the default values for the persistence unit.7.Click Finish.When using the wizard to create entity classes from a database, the IDE examines the relationships between the tables. In the Projects window, if you expand the entities Source Package node you can see that the IDE generated an entity class for each table except for the PROJECT_CONSULTANT table. The IDE did not create an entity class for PROJECT_CONSULTANT because the table is a join table.The IDE also generated two additional classes for the tables with composite primary keys: CLIENT and PROJECT. The primary key classes for these tables (ClientPK.java and ProjectPK.java) have PK appended to the name.Editing the Entity ClassesNow that the entity classes are generated, you need to make small changes to the entity classes to add code not generated by the wizard. By adding the code before you create the JSF pages from the classes, you take advantage of the improved code generation functionality in the wizard.When creating the entity classes, the Entity Classes from Database wizard does not add@GeneratedValue annotations or optional annotation elements to the entity classes. By adding the optional annotation elements, the JSF Pages from Entity Classes wizard can generate code that includes checks to prevent non-nullable column violations.In this exercise you make the following changes to the default code generated by the Entity Classes from Database wizard.∙Apply a @GeneratedValue annotation to any ID field that corresponds to an autogenerated primary key database columnIf you look at the SQL script you used to create the database, you can see that for some of the primary key columns in the tables the GENERATED ALWAYS AS IDENTITY keywords are used to indicate that the columns are autogenerated.∙Apply the annotation element (optional = false) on all non-nullable many-to-one (@ManyToOne) and one-to-one (@OneToOne) relationshipsAdding this element will cause the IDE to generate required checks when you create the JSF pages using the JSF Pages from Entity Classes wizard. By generating required checks, you prevent the user from attempting to use a null value in a non-nullable database column.Customize the implementations of the toString method in the entity classes In the Client and Recruiter classes you will also modify the annotations to change the relationship between clients and recruiters from one-to-many to one-to-one. Because a nullable one-to-many relationship already exists between recruiters and consultants, for demonstration purposes in this tutorial you will establish a nullable one-to-one relationship between clients and recruiters.Editing Address.javaOpen the Address entity class and make the following changes.1.Mark the addressId field as generated by adding the @GeneratedValue annotationbeneath the @Id annotation.2.private static final long serialVersionUID = 1L;3.@Id4.@GeneratedValue(strategy = GenerationType.IDENTITY)5.@Column(name = "ADDRESS_ID", nullable = false)private Integer addressId;You can use the IDE's code completion to help you add annotations and annotationelements. When you use code completion, the IDE automatically adds the appropriateimport statement for you.6.Modify the return statement of the toString method to return the full address:return line1 + ", " + line2 + ", " + city + ", " + region + ", " + country + ", " + postalCode;You can use the IDE's code completion to help you add the fields.7.Fix your imports, if necessary, and save your changes.Editing Billable.javaOpen the Billable entity class and make the following changes.1.Mark the billableId field as generated by adding the @GeneratedValue annotationbeneath the @Id annotation.2.private static final long serialVersionUID = 1L;3.@Id4.@GeneratedValue(strategy = GenerationType.IDENTITY)5.@Column(name = "BILLABLE_ID", nullable = false)private Long billableId;6.Modify the @ManyToOne annotation on the consultantId and project fields to add the(optional = false) element.7.@JoinColumn(name = "CONSULTANT_ID", referencedColumnName ="CONSULTANT_ID")8.@ManyToOne(optional = false)9.private Consultant consultantId;10.@JoinColumns({@JoinColumn(name = "CLIENT_NAME",referencedColumnName = "CLIENT_NAME"), @JoinColumn(name ="CLIENT_DEPARTMENT_NUMBER", referencedColumnName ="CLIENT_DEPARTMENT_NUMBER"), @JoinColumn(name = "PROJECT_NAME",referencedColumnName = "PROJECT_NAME")})11.@ManyToOne(optional = false)private Project project;12.Modify the return statement of the toString method to return the description,consultant id and project:13.public String toString() {return description + ", " + consultantId + ", " + project;14.Fix your imports, if necessary, and save your changes.Editing Client.java1.Modify the @OneToMany annotation on the recruiterCollection field to make therelationship one-to-one and change the field from a Collection to Recruiterrecruiter.2.@OneToOne(mappedBy = "client")private Recruiter recruiter;3.Modify the getters and setters for Collection<Recruiter> recruiterCollectionaccording to the changes in the name of the field. The new getter and setter will look like the following:4.public Recruiter getRecruiter() {5. return recruiter;6.}7.8.public void setRecruiter(Recruiter recruiter) {9. this.recruiter = recruiter;}It may be simpler to delete the getter and setter for Collection<Recruiter>recruiterCollection and then generate a new getter and setter for Recruiterrecruiter using the Insert Code dialog. To create a new getter and setter, right-click in the source code and choose Insert Code (Alt-Insert) and then select Getter and Setter.Select recruiter in the Generate Getter and Setter dialog box and click Generate.10.Modify the @OneToOne annotation on the billingAddress field to add the (optional= false) property.11.@JoinColumn(name = "BILLING_ADDRESS", referencedColumnName ="ADDRESS_ID")12.@OneToOne(optional=false)private Address billingAddress;13.Modify the return statement of the toString method to returnString.valueOf(clientPK):14.public String toString() {15. return String.valueOf(clientPK);}16.Fix your imports, if necessary, and save your changes.Editing ClientPK.java1.Modify the return statement of the toString method to return the client name anddepartment number:2.public String toString() {3. return clientName + ", " + "Dept. " + clientDepartmentNumber;}4.Save your changes.Editing Consultant.java1.Mark the consultantId field as generated by adding the @GeneratedValue annotationbeneath the @Id annotation.2.private static final long serialVersionUID = 1L;3.@Id4.@GeneratedValue(strategy = GenerationType.IDENTITY)5.@Column(name = "CONSULTANT_ID", nullable = false)private Integer consultantId;6.Modify the @ManyToOne annotation on the statusId field to add the (optional =false) property.7.@JoinColumn(name = "STATUS_ID", referencedColumnName = "STATUS_ID")8.@ManyToOne(optional=false)private ConsultantStatus statusId;9.Modify the return statement of the toString method to return the email:10.public String toString() {11. return email;}12.Fix your imports, if necessary, and save your changes.Editing ConsultantStatus.java1.Modify the return statement of the toString method to return the status ID anddescription:2.public String toString() {3. return statusId + ", " + description;}4.Save your changes.Editing Project1.Modify the @ManyToOne annotation on the client field to add the (optional = false)property.2.@JoinColumns({@JoinColumn(name = "CLIENT_NAME",referencedColumnName = "CLIENT_NAME", insertable = false, updatable = false), @JoinColumn(name = "CLIENT_DEPARTMENT_NUMBER",referencedColumnName = "CLIENT_DEPARTMENT_NUMBER", insertable = false, updatable = false)})3.@ManyToOne(optional=false)private Client client;4.Modify the return statement of the toString method to return the following:5.public String toString() {6. return (projectPK == null ? "(unnamed project)" :projectPK.getProjectName()) + ", " + client;}7.Save your changes.Editing Recruiter.java1.Mark the recruiterId field as generated by adding the @GeneratedValue annotationbeneath the @Id annotation.2.private static final long serialVersionUID = 1L;3.@Id4.@GeneratedValue(strategy = GenerationType.IDENTITY)5.@Column(name = "RECRUITER_ID", nullable = false)private Integer recruiterId;6.Modify the @ManyToOne annotation on the client field to mark the relationship asone-to-one.7.@OneToOneprivate Client client;8.Modify the return statement of the toString method to return the email:9.public String toString() {10. return email;;}11.Fix your imports, if necessary, and save your changes.Generating JSF Pages From Entity ClassesNow that the classes are created, you can create a web application for displaying and modifying the data. You will use the JSF Pages from Entity Classes wizard to generate a JavaServer Faces application from the entity classes. For each entity class the wizard will generate the following: ∙ a controller class∙ a converter class∙ a directory containing four JSP files (Detail.jsp, Edit.jsp, List.jsp, New.jsp) ∙managed bean elements, converter elements and navigation rules for the class in faces-config.xmlThe JSF Pages from Entity Classes wizard will generate code that includes checks based on the modifications you made to the entity classes.1.Right-click the project node in the Projects window and choose New > JSF Pages fromEntity Classes to open the New JSF Pages from Entity Classes wizard.The Available Tables pane of the wizard lists the seven entity classes in the project. Thewizard does not list the embeddable classes (ClientPK.java and ProjectPK.java)2.Click Add All to move all the classes to the Selected Entity Classes pane of the wizard. ClickNext.3.Type beans for the Package. Click Finish.When you click Finish, the IDE creates the converter and controller classes for each entity class in the beans package. Each controller class handles the operations for the corresponding entity class, including creating, editing, and destroying instances of the entity class via the Java Persistence API. Each converter class implements the javax.faces.convert.Converter interface defined by JavaServer Faces and performs the work of converting instances of the corresponding entity class to String objects and vice versa.If you expand the Web Pages node, you can see that the IDE generated a folder for each of the entity classes. Each folder contains the files Detail.jsp, Edit.jsp, List.jsp and New.jsp. The IDE also modified welcomeJSF.jsp to insert links to each of the List.jsp pages.If you expand the Configuration Files node and open faces-config.xml in the XML editor, you can see that the IDE inserted the managed-bean and converter elements for each of the controller and converter classes. The IDE also inserted a navigation-rule element for each JSP, indicating the logical outcome that causes the application to navigate to that JSP.Running the ProjectNow that you have finished creating the application, you can deploy and run the application.1.Click Run Main Project in the main toolbar.The IDE saves all changed files, rebuilds the application, and deploys the application to the server. Your default web browser opens to the local addresshttp://localhost:8080/ConsultingAgency/.2.Click the links to view and add details.When you start clicking the links and adding data, you can see how the relationships between the tables function.。