iela_practicetest
ielts selt consortium test of english
IELTS SELT Consortium Test of EnglishIntroductionThe IELTS SELT (Secure English Language Test) Consortium Test of English is an internationally recognized English language proficiency test. Itis designed to assess the language skills of non-native English speakers who need to prove their proficiency in English for various purposes, such as studying abroad, immigrating to English-speaking countries, or applying for certain professional certifications. In this article, we will explore the details of the IELTS SELT Consortium Test of English and discuss its significance and benefits for test takers.Overview of the IELTS SELT ConsortiumThe IELTS SELT Consortium is a collaboration between the International English Language Testing System (IELTS) and SELT Providers approved by the UK government. SELT Providers administer and conduct the IELTS SELT Consortium Test of English in accordance with the established standards and guidelines. The test evaluates the four language skills: Listening, Reading, Writing, and Speaking.Listening Section•The Listening section of the test assesses a candidate’s ability to understand spoken English in various contexts, such asconversations, lectures, and discussions.•Candidates listen to audio recordings and answer a series of questions based on the information they hear.•The section comprises multiple-choice, matching, and sentence completion questions.Reading Section•The Reading section evaluates a candidate’s comprehension and interpretation of written English.•Candidates are presented with a variety of texts, including articles, advertisements, and opinion pieces, and they must answer questions based on the information in the texts.•The section includes multiple-choice, matching, and True/False/Not Given questions.Writing Section•The Writing section tests a candidate’s abili ty to communicate effectively in written English.•Candidates are required to complete two writing tasks, usually an essay and a letter/report.•The tasks assess the candidate’s ability to organize ideas, express opinions, and provide coherent arguments.Speaking Section•The Speaking section examines a candidate’s oral communication skills in English.•Candidates engage in a face-to-face conversation with an examiner, during which they are evaluated on their ability to express ideas, engage in a discussion, and respond appropriately to questions.•The section includes various tasks, such as describing a photograph, discussing a topic, and expressing opinions.Significance of the IELTS SELT Consortium TestThe IELTS SELT Consortium Test of English holds significant importance for individuals who require proof of English language proficiency for specific purposes. Here are some key reasons why this test is valuable:Worldwide Recognition•The IELTS SELT Consortium Test is recognized by thousands of educational institutions, employers, and immigration authoritiesworldwide.•The test certificate serves as evidence of an individual’s English proficiency, opening doors to academic opportunities, job prospects, and visa applications.Comprehensive Assessment•The test assesses all four language skills, ensuring a comprehensive evaluation of a candidate’s English languageabilities.•By examining listening, reading, writing, and speaking skills, the test provides a comprehensive profile of an individual’s language proficiency level.Valid and Reliable•The IELTS SELT Consortium Test is developed by experts in the field of language assessment, ensuring its validity andreliability as a measure of English proficiency.•The test items are carefully designed to accurately reflect real-life language use and effectively differentiate between different levels of language competency.Tailored to Specific Needs•The IELTS SELT Consortium Test offers different versions tailored to specific purposes, such as IELTS Academic for higher education and IELTS General Training for immigration and work.•This allows individuals to choose the appropriate test version that matches their specific requirements.Preparation Strategies for the IELTS SELT Consortium TestTo achieve a satisfactory score in the IELTS SELT Consortium Test of English, proper preparation is crucial. Here are some strategies that can help individuals prepare effectively:Familiarize Yourself with the Test Format•Understanding the test format is essential, as it allows candidates to know what to expect in each section and how toallocate their time.•Review sample test materials and become familiar with the types of questions and tasks in each section.Develop Language Skills•Enhance your listening and reading skills by regularly practicing with authentic materials, such as podcasts, news articles, andacademic texts.•Improve your writing skills by practicing different types of essays and letters and seeking feedback from experienced Englishteachers or tutors.•Enhance your speaking skills by engaging in conversations with native English speakers or participating in language exchangeprograms.Time Management•Time management is crucial in the IELTS SELT Consortium Test, as each section has a specified time limit.•Practice completing tasks within the allocated time to develop a sense of timing and improve your efficiency during the test.Mock Tests and Feedback•Take advantage of practice tests and mock exams to simulate the test environment and assess your performance.•Seek feedback from knowledgeable individuals or join study groups to identify areas for improvement and implement effectivestrategies.ConclusionThe IELTS SELT Consortium Test of English is a reputable and widely recognized assessment of English language proficiency. By evaluating the listening, reading, writing, and speaking skills of candidates, it provides a comprehensive measure of their language abilities. Understanding the significance of this test and adopting effective preparation strategies can significantly enhance the chances of success for individuals aiming to prove their English proficiency for educational, professional, or immigration purposes.。
CLAD 2014 模拟卷 2
LabVIEW助理开发工程师考试模拟试题注:考试过程中不允许使用计算机或其他参考资料。
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1._____2._____3._____4._____5._____6._____7._____8._____9._____10._____11._____12._____13._____14._____15._____16._____17._____18._____19._____20._____21._____22._____23._____24._____25._____26._____27._____28._____29._____30._____31._____32._____33._____34._____35._____36._____37._____38._____39._____40._____Q1: 下列哪个用户界面事件可使代码在LabVIEW执行该事件相关的默认操作前响应?A 鼠标按下B 前面板大小调整C 前面板关闭?D 值改变Q2: 将触发控件配置为转换的机械动作。
VI要求显示数量值,用于追踪触发发生“值改变”(值变为TRUE)事件的次数。
TKT-module-2-sample测试题
INFORMATION FOR CANDIDATES There are 80 questions in this paper. Each question carries one mark.
PV5 © UCLES 2015
2 For questions 1 – 6, match the activities for teaching writing with the main teaching focuses listed A – G. Mark the correct letter (A – G) on your answer sheet. There is one extra option which you do not need to use.
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The main aim of Stage 1 is to A B C introduce key themes for the unit. present a set of proper nouns. review structures from a previous unit.
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5 15 The main aim of Stage 2 is to A B C remind learners of structures they will need for the activity. assess learners’ own experiences. stimulate learners’ interest in the topic.
3 4 5 6
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3 For questions 7 – 13, match the stages of a lesson with the main stage aims listed A – I. Mark the correct letter (A – I) on your answer sheet. There is one extra option which you do not need to use.
检验测试程序英文版
Quality Procedure Testing & InspectionTable of Contents1TESTING & INSPECTION (3)1.1I NTRODUCTION &P URPOSE (3)1.1.1Process Activity Map (3)1.1.2References ..............................................................................................................................................................................1.1.3Terms & Definitions (3)1.2A PPLICATION &S COPE (4)1.3R ESPONSIBILITIES (4)1.4T ESTING &I NSPECTION P ROCESS (4)1.4.1General ...................................................................................................................................................................................1.4.2Receiving Inspection (4)1.4.3First Article Inspection (5)1.4.4In-process Inspection (5)1.4.5Final Inspection ....................................................................................................................................................................1.4.6Non-conformities (6)1.5F ORMS &R ECORDS (6)1.6T ESTING &I NSPECTION P ROCESS M AP (7)1Testing & Inspection1.1Introduction & PurposeThe purpose of this procedure is to establish and define the process for testing and inspection activities that verify product, material and service conformance, and to verify that process inputs and outputs conform to specified requirements. Documented Records and information of inspection include evidence of conformity with the acceptance criteria and traceability to the person authorizing the release. Records of inspection are maintained.1.1.1Process Activity Map1.1.2ReferencesStandardTitleDescriptionBS EN ISO 9000:2015 Quality management systems Fundamentals and vocabulary BS EN ISO 9001:2015 Quality management systems RequirementsBS EN ISO 9004:2018Quality management systemsGuidelines for performance improvements1.1.3Terms & DefinitionsTerm DefinitionInspection Conformity evaluation by observation/judgement/appropriate measurement or testing Test Determination of one or more characteristics according to a procedureVerificationConfirmation, through the objective evidence (3.8.3), requirements (3.6.4) were fulfilledOutput?Validated products?Signed inspection records ?Non-conformance reports ?Calibration certificates and logs?Traceability to material and process certificates ?How?Measurement & analysis ?Inspections?Comparison to criteriaWith what measure?Critical tolerances ?Acceptance criteria ?Customer requirementsWith what?Calibrated measuring and monitoring devices ?Incoming materials?Documents and drawings ?In-process articles ?Finish articlesWith who?Operations Manager ?QC Inspectors ?Top management ?QEHS Manager ?Production TeamsActivityInput?Stock and materials ?Customer specifications ?Technical data ?Inspection plan ?Control plans?Contract requirements ?Control plans ?Define methods of inspecting products, materialand services to ensure conformance with requirements。
PracticeTestOne
PracticeTestOnePractice Test OneSection ADirections: In this section, you will hear three news reports. At the end of each news report, you will hear two or three questions. Both the news report and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A, B, C and D.News item 1Questions 1 and 2 are based on the news report you have just heard.1. A. Car sales grew rapidly. B. Car sales declined as a whole.C. Gas prices went up.D. Demand for trucks and SUVs rose.2. A. Rise in car prices. B. Decline in gas prices.C. Overall drop in auto stocks.D. Increased customer need to buy cars.News item 2Questions 3 and 4 are based on the news report you have just heard.3. A. Rich people tend to be less anxious than poor people.B. Men are less likely to experience anxiety than women.C. People from North America are unlikely to suffer from anxiety.D. People having heart diseases tend to worry more than people having cancer.4. A. Potentially stressful situations.B. Unpredictable worry or fear.C. Physical problems related to health.D. Mental health problems quite common in the West.News item 3Questions 5 to 7 are based on the news report you have just heard.5. A. A few hours after a wildfire broke out.B. When much of Alberta was destroyed.C. After oil companies in Alberta were forced to cut their output.D. After a wildfire forced all the residents in Fort McMurray to flee their city.6. A. Because there were no enough staff working.B. Because much of the equipment had been destroyed.C. Because those not in key positions could therefore leave the area.D. Because many pipelines had caught fire, thus threatening people there.7. A. It destroyed many buildings.B. It caused many deaths and injuries.C. It made many people jobless.D. It was held under control a few hours after it broke out.Section BDirections: In this section, you will hear two long conversations. At the end of each conversation, you will hear four questions. Both the conversation and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A, B, C and D.Conversation 1Questions 8 to 11 are based on the conversation you have just heard.8. A. He’s going to teach in Scotland.B. He’s going back home to Scotland.C. He’s got tired of teaching in the department.D. He’s not on speaking terms with most of his colleagues.9. A. He’s quite demanding.B. His lectures are very interesting.C. He’s very enthusiastic about department activities.D. He’s pretty hard on the students who get poor grades.10. A. They should get extremely high grades.B. They should go to parties quite often.C. They should work hard to meet high standards.D. They should go over lecture notes before the final exams.11. A. It depresses him. B. It stops him from going to classes.C. It makes him play sports more.D. It makes him work harder.Conversation 2Questions 12 to 15 are based on the conversation you have just heard.12. A. One of a very old model. B. One of a famous brand.C. One with basic functions.D. One with sophisticated functions.13. A. If it’s too big, he may break it.B. If it’s too sma ll, he may drop it.C. It will be quite convenient to be carried around.D. It will be too heavy or he may lose it in either case.14. A. It is lightweight. B. It is the newest model.C. It can be used worldwide.D. It can be used in the car.15. A. The battery will last longer. B. It is quite efficient to use.C. It is quite small in size.D. The screen is quite large.Section CDirections: In this section, you will hear three passages. At the end of each passage, you will hear some questions. Both the passage and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A, B, C and D. Passage 1Questions 16 to 18 are based on the passage you have just heard.16. A. Various oils used in cooking.B. Ways of protecting food from insects.C. The behavior of a kind of beetle.D. Smells produced by different grasses.17. A. They were very heavy.B. They did not contain much food.C. Beetles avoided them.D. Many insects were trapped in them.18. A. How safe it is.B. What chemicals it has.C. Where it comes from.D. Why beetles like it.Passage 2Questions 19 to 21 are based on the passage you have just heard.19. A. To develop a new type of transport.B. To improve the efficiency of current transportation system.C. To find out whether transportation systems in big cities are too complex.D. To compare different types of transportation.20. A. 15. B. 250. C. 25. D. 235.21. A. Redesigning the maps.B. Making better use of public transport.C. Handing out more brochures.D. Asking more people for help.Passage 3Questions 22 to 25 are based on the passage you have just heard.22. A. The palolo. B. The spider. C. The lion. D. The herring gull.23. A. The social organization in which pairs bring up their young.B. How many chicks each pair raise.C. How long they spend together.D. Whether the male and female raise the chicks.24. A. They live separately and come together for some activities.B. They live in a group and do all activities together.C. They spend the day separately and the night together.D. They live in a group and do some activities together.25. A. They are made up of male and female bees.B. They could not survive alone.C. They carry out different tasks depending on their age.D. They live in a social structure unlike that of any other animals.Practice Test OneScript and Answer KeySection ADirections: In this section, you will hear three news reports. At the end of each news report, you will hear two or three questions. Both the news report and the questions will be spoken only once. After you hear a question, you must choose the bestanswer from the four choices marked A, B, C and D.News item 1Most major car makers reported lousy(糟糕的)sales in May. Sales fell at GM, Ford, Toyota and Honda. Fiat Chrysler was the only major auto company to buck the trend, extending the company’s impressive sales growth for 74 consecutive months. Still, sales were up only 1.1% though, led mainly by strong demand for its Jeep brand.Up until recently, the auto industry has been a bright spot for the U.S. economy. Sales hit a record last year, thanks to robust demand for trucks and SUVs as gas prices remained low. But the slowdown in May is a troubling sign—especially now that energy prices have surged in the past few months. This has taken a toll on auto stocks. Ford is down more than 6% this year. GM has fallen 11%. Toyota and Honda are each down about 15% and Fiat Chrysler has plunged nearly 25%. With gas prices increasing, consumers may also increasingly choose to lease a car instead of buying one.Questions 1 and 2 are based on the news report you have just heard.1. What happened to most major car companies in May?2. According to the news report, what has the recent slowdown in the auto industry brought about?KEY1. B2. CNews item 2Women are almost twice as likely to experience anxiety as men, according to a review of existing scientific literature, led by the University of Cambridge. The study also found that peoplefrom Western Europe and North America are more likely to suffer from anxiety than people from other cultures. The review, published today in the journal Brain and Behavior, also highlighted how anxiety disorders often provide a double burden on people experiencing other health-related problems, such as heart disease, cancer and even pregnancy.Anxiety disorders, which often manifest as excessive worry, fear and a tendency to avoid potentially stressful situations including social gatherings, are some of the most common mental health problems in the Western world. The annual cost related to the disorders in the United States is estimated to be $42.3 million. In the European Union, over 60 million people are affected by anxiety disorders in a given year.Questions 3 and 4 are based on the news report you have just heard.3. According to the news report, which of the following is true?4. Which of the following does anxiety disorder refer to?KEY3. B4. DNews item 3A state of emergency has been declared in the province of Alberta in Canada after a wildfire forced all 88,000 residents of Fort McMurray to flee.Officials say the fast-moving blaze could destroy much of the city.The fire, which broke out on Sunday in the heart on the country’s oil sands region, has gutted (摧毁)1,600 buildings, including a new school.The evacuation(疏散)was largest-ever in Alberta. Oil companies operating in the area have been forced to cut output.Several firms have shut down some pipelines. This was done to help evacuate nonessential personnel, reports say.So far there have been no reports of deaths or injuries in the wildfire, but two women gave birth in one evacuation center, Reuters reported.Bernie Schmitte, an official at Alberta’s Agriculture Ministry, said on Wednesday that the “catastrophic fire” had so far “resisted all suppression methods.”First Nation communities 30 miles south of the city were given mandatory(强制性的)evacuation orders on Wednesday.Questions 5 to 7 are based on the news report you have just heard.5. When was a state of emergency declared in Alberta, Canada?6. Why did some oil companies temporarily close some of their pipelines?7. According to the news report, which of the following is mentioned as a consequence of the Alberta fire?KEY5. D6. C7. ASection BDirections: In this section, you will hear two long conversations. At the end of each conversation, you will hear four questions. Both the conversation and the questions will be spoken only once. After you hear a question, you must choose the best answer from the four choices marked A, B, C and D.Conversation 1M: Have you heard that Professor John is going to be leaving the department soon?He’s going back home to Scotland.W: Really? I didn’t know that.M: Were you ever in one of his classes?W: No, but I’ve heard a lot about the guy. Seems he’s a pretty demanding type. What do you think of him as a lecturer?M: He certainly knows his stuff, and his lectures are quite interesting. But he has extremely high standards and expects everyone to meet these standards. Of course not everyone is that capable.W: So you’re saying he’s pretty hard on the students who don’t get good grades?M: Well, it’s more than that. You kind of get the feeling that he looks down on you if you’re not a genius.W: Yeah, but university students should really be able to perform to a high level. His problem is not with the ability of some students, but with their attitude towards study.M: There certainly are people who don’t work as hard as they could. You know, always playing sports, going to parties, etc.W: How about you? What was he like to you?M: Not too bad, because I could always come up with some kind of answer for any question he put to me.W: So do you think that kind of pressure made you work harder in that subject?M: I suppose it did really. There was always the thought in the back of your mind that if you didn’t prepare properly for Professor John’s lectures then there was a chance you’d end up looking silly during the classes.W: So it sounds like the extra pressure actually works.M: Well, maybe for me. Maybe more people should change their attitude towards study. I really feel sorry to see the old fellow leave myself.Questions 8 to 11 are based on the conversation you have just heard.8. Why is Professor John leaving the department?9. What does the female student think of Professor John?10. What does the female student think university students should do?11. What effect does extra pressure have on the male student?KEY8. B 9. A 10. C 11. DConversation 2M: Excuse me, can you give me some information about purchasing a cellular phone?W: Of course, my pleasure. I will do my best to help you find a phone that suits your needs.M: Thanks. I won’t need anything too sophisticated, just your basic phone functions.W: Sure, let’s take a look. What about size, color or the brand?M: Well, I don’t really care what brand the cell phone is. But I don’t want a nything that is too big or too small. I want a phone that can fit nicely in my hand and in my pocket. If it is too big it might be too heavy, and if it is too small I might lose it. Color I don’t really care about either.Well, I don’t want a pink phone.W: OK. How about this one? This one is the R55, black, not too big, not too small, all the usual functions. The best feature ofR55 is that it can be used worldwide, even in Europe or Asia. M: It looks good. How much does it cost?W: Only $100.M: How old i s this model though? I don’t want anything too old.W: This model was introduced to the market about 3 years ago, so it is a bit older, but be assured, it will still work fine.M: Well, I still want something not as old. How about from last year? Any good phones from around that time?W: Yes, there are some. How about this one? It’s the new model of the phone you just looked at, called the W55. Most of the features are the same. There are some new features on the W55 though. The battery will last up to 2 days longer, and the overall weight of the phone is lighter. M: How much is this one?W: $150.M: OK, I think I will take this one.Questions 12 to 15 are based on the conversation you have just heard.12. What kind of cellular phone does the man want?13. Why does the man want a phone neither too big nor too small?14. What is the best feature of the R55 phone?15. What advantage of the W55 phone is mentioned in the conversation?KEY12. C 13. D 14. C 15. ASection CDirections: In this section, you will hear three passages. At the end of each passage, you will hear some questions. Both the passage and the questions will be spoken only once. After youhear a question, you must choose the best answer from the four choices marked A, B, C and D.Passage 1The longer food is kept, the more likely it is to attract insects. Even foods stored in containers often attract bugs. To solve this problem, scientists have been working with different odors in an attempt to find one strong enough to keep insects from going near food. One possibility would be to use plants with strong smells, like garlic or pine, to keep insects away. Unfortunately, however, using these smells might keep some people away too!A more promising repellent(防护剂)is citronella oil, which comes from a type of lemongrass. An experiment was done using this oil with a certain insect, the red flour beetle. Scientists sprayed cardboard boxes with citronella oil and noticed that the beetles did not enter those boxes. They were much more interested in boxes that were not sprayed.One problem with using citronella oil as a repellent, however, is that it is quite ephemeral—it simply does not last very long. After a few months it loses its smell, and bugs no longer find it unpleasant. Scientists hope to improve citronella oil so that its scent remains strong for a longer time. It will also be necessary to make sure that the oil is not harmful to people, as scientists are still not sure whether it is safe to use around food.Questions 16 to 18 are based on the passage you have just heard.16. What is the passage mainly about?17. What does the speaker say about the sprayed boxes?18. What do scientists still not know about citronella oil?KEY16. B 17. C 18. APassage 2Many of us know the feeling of standing in front of a subway map in a strange city, puzzled by the multi-colored web and seemingly unable to find a route from point A to point B.Now, a team of physicists and mathematicians has attempted to quantify this confusion and find out whether there is a point at which navigating a route through a complex urban transport system exceeds our cognitive limits.After analyzing the world’s 15 largest metropolitan(大都市的)transport networks, the researchers estimated that there is the cognitive limit for planning a trip. Additionally, this cognitive limit for transportation suggests that maps should not consist of more than 250 connection points to be easily readable.Using journeys with exactly two connections as their basis, the researchers found that navigating transport networks in major cities can come close to exceeding humans’cognitive powers.Human cognitive capacity is limited, and cities and their transportation networks have grown beyond human processing capability. In particular, the search for a simplest path becomes inefficient when a transportation system has too many interconnections. Put simply, the maps we currently have need to be rethought and redesigned in many cases. Journey-planner apps of course help, but the maps themselves need to be redesigned.Questions 19 to 21 are based on the passage you have just heard.19. What is the purpose of the team of experts?20. What is the maximum number of connections for maps to be easily readable?21. What do the experts suggest doing to help search for a simplest path?KEY19. C 20. B 21. APassage 3The palolo—a worm which lives on rocks in the sea—is one of very few animals which never have contact with other members of the same species. Others, such as spiders, are normally solitary, meeting only to mate (that is, to reproduce).Some species form social links only for the period while they are rearing their young. Among birds, European robins raise their chicks in a pair, away from other members of their species, while herring gulls form larger groups (colonies) consisting of many pairs living close together, each pair raising their chicks independently.Many species of fish and birds form large groups, called schools and flocks, respectively, and swim or fly together. Hens attack each other, and eventually establish a hierarchy(等级制度)based on their individual strength. Those at the top of the “pecking order” get to eat before the others.Finally, some animals spend most or all of their lives in social groups in which individuals co-operate. Lions, for instance, usually live in a relatively permanent group, called a pride, where some activities, such as hunting, are social and others, like sleeping, are solitary.Bees, wasps and ants live in stable, co-operative groups in which every activity is communal and organized. Worker bees (which are all female) have several jobs in succession, depending on their age. They begin with cleaning duties, and later become soldiers to defend the hive against intruders. Finally they fly outof the hive to collect food. Theirs is a highly complex social organization.Questions 22 to 25 are based on the passage you have just heard.22. Which of the following animals spends most, but not all its life, alone?23. In what way are European robins different from herring gulls?24. What is said about the life of lions?25. What point is made about worker bees?KEY22. B 23. A 24. D 25. C。
Pearson Edexcel GCE Music Technology 设置和操作指南说明书
Turn over*P46734A0112*P46734A©2016 Pearson Education Ltd.1/1/1/1/1/1Setting up time1. Open a new project in the music production software using 16 bit/44.1kHz sample rate.2. Save the project as unit4_your candidate number (e.g. unit4_1234) in the folder designatedby your centre.3. Set the metronome to 128 bpm.4. Import “drums.wav” from the CD ROM to a stereo audio track in the music production software,aligned with the beginning of bar 1.5. Ensure that the drums are audible and play in time with the metronome. The drums beginplaying in bar 2.Instructions• Useblack ink or ball-point pen.• Fill in the boxes at the top of this page with your name, centre number and candidate number.• Answerall questions.• Write your answers to Section A in the spaces provided in this question paper.• You must save your exported audio files for Questions 2 & 3 in Section A, and Question 5 in Section B to your project folder within the 2 hour examination time.• You must ensure that the left and right earpieces of your headphones are worn correctly.• Access to the internet or local network is not permitted.Information• The total mark for this paper is 80.• The marks for each question are shown in brackets–use this as a guide as to how much time to spend on each question.• Questions labelled with an asterisk (*) are ones where the quality of your written communication will be assessed– you should take particular care on these questions with your spelling, punctuation and grammar,as well as the clarity of expression.Advice• Read each question carefully before you start to answer it.• Try to answer every question.• Check your answers if you have time at the end.2DO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREA Import “synth chords.wav” from the CD ROM to a new stereo track in your music*P46734A0212*3 DONOTWRITEINTHISAREADONOTWRITEINTHISAREADONOTWRITEINTHISAREA*P46734A0312*Turn over4DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA*P46734A0412*5 DONOTWRITEINTHISAREADONOTWRITEINTHISAREADONOTWRITEINTHISAREA*P46734A0512*Turn over6DO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREA(d) (i) On the graph below, draw the waveform identified in part (c).(1)(ii)Labeltheaxes.(2)(iii) Label the amplitude of the wave.(1)(iv) Label the wavelength of the wave.(1)(e) On the blank graph below, draw the same wave with the phase inverted.(1)(f) Describe what would happen if the waveforms from parts (d) and (e) were addedtogether.(1) ....................................................................................................................................................................................................................................................................................*P46734A0612*7 DONOTWRITEINTHISAREADONOTWRITEINTHISAREADONOTWRITEINTHISAREA*P46734A0712*Turn over8DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA*P46734A0812*9 DONOTWRITEINTHISAREADONOTWRITEINTHISAREADONOTWRITEINTHISAREA............................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................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for Question 4 = 16 marks)TOTAL FOR SECTION A = 62 MARKS*P46734A0912*Turn over10DO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREA SECTION B5You should now have the following tracks imported on the computer: drums, synth chords, bass and vocals.Follow the instructions below to produce a final stereo mix.(a) Apply automated panning to the drums.• Onlybars 4 and 5 should be affected; all other bars should be panned to the centre.• The hand clap in bar 4 should be panned hard left.• The hand clap in bar 5 should be panned hard right.• The bass drum should be panned centre throughout.(3)(b) Listen to the automated EQ on the vocals in bars 18–25. Recreate that EQ inbars 2–9. You will need to cut and paste bars 2–9 on to a new track becauseapplying EQ may reintroduce the unwanted tone.(3)(c) Compressthesynth chords.• Onlybars 18–25 should be affected.• The drums should trigger the side-chain of the compressor so that the synth chords part pumps in time with the bass drum.• The side-chained compression should suit the style of the music.(3)(d) Apply a mono delay effect to the synth chords.• Use a crotchet synced delay.• The delay should fill the gaps in the introduction.• The delay should be clearly audible.• Ensure that the delay is not intrusive.(3)*P46734A01012*11 DONOTWRITEINTHISAREADONOTWRITEINTHISAREADONOTWRITEINTHISAREA*P46734A01112*12DO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREADO NOT WRITE IN THIS AREA BLANK PAGE*P46734A01212*。
ILTS测评的使用方法
总结与回顾
1
邦国教育 ILTS测评 简介
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流 程
E-ILTS(E-Personalized Private Tutoring System), 其核心流程为 “个性化分析诊断报告→找出学习优势和类型→定制教学辅导方案→ 匹配适合的一对一学科授课教师→构建个性学习方法策略→教研团队 跟踪管理→定期测评→回访反馈。”
非智力因素
自信心与好强心(自信心与成就动机)
努力——以努力程度间接测试学生对自己能力的信心 能力——直接测试学生对自己能力的信心
首先,合理设计目标,让学生学会自我强化。
其次,对失败要有合理的归因,维护其对自身能力的信心。 最后,会议过去的成功体验,磨练意志,善用学习策略。
好强心——成就动机(包括挑战性、竞争性等)的弱项
智力因素
记忆力——艾宾浩斯遗忘曲线 德国心理学家艾宾浩斯 (Hermann Ebbinghaus )对遗忘 现象做了系统的研究他用无意义的 音节作为记的材料,把实验数据绘制成 一条曲线,称为艾宾浩斯遗忘曲线。这条曲线一般称为艾宾 浩斯遗忘曲线,也称为艾宾浩斯保持曲线,它的纵坐标代表保持 曲线表明了遗忘发展的一条规律:遗忘的进程不是均衡的,不是固 定的一天丢掉几个,,转天又丢掉几个的,而是在记忆的最初 阶段遗忘的速度很快,后来就逐渐减慢了,到了相当长的时候 后,几乎就不再遗忘了,这就是遗忘的发展规律,即“先快后 慢”的原则。
习惯。现代学生先天性的智力水平大致相近,而最终造成学生不同成绩的原因, 主要是非智力因素,ILTS测评是基于这个研究结果,系统着艳于学生的非智力因 素,指出影响学习的要素——智力
目录
间接ELISA检测方法
间接ELISA检测⽅法ARTICLEDevelopment of an indirect ELISA based on a truncated S protein of the porcine epidemic diarrhea virusYufeng Li,Fangyuan Zheng,Baochao Fan,Hassan Mushtaq Muhammad,Yao Zou,and Ping JiangAbstract:Porcine epidemic diarrhea (PED)is a highly contagious,enteric disease of swine caused by the porcine epidemic diarrhea virus (PEDV).To ?nd a suitable ELISA method to assess the infection of PEDV and the effective-ness of vaccines,we developed and evaluated an indirect enzyme-linked immunosorbent assay (iELISA)based on a truncated recombinant spike (S)protein expressed in Escherichia coli .The parameters of the iELISA were opti-mized,and the cutoff value determined as 0.259by analyzing optical density (OD)values of 80PEDV negative sera con?rmed by westernblot.Repeatability tests revealed that the coef?cients of variation of positive sera within and between runs were both less than 10%.Cross-reactivity assays demonstrated that iELISA was PEDV-speci?c.A virus neutralization test with sera of 7different OD values showed a positive correlation between the OD values and virus neutralization.The results suggest this iELISA is speci?c,sensitive,and repeatable.Further studies should focus on the relationship between OD values of sera and its virus neutralization.Key words:indirect ELISA,PEDV,truncated S protein,virus neutralization.Résumé:La diarrhée épidémique porcine (DEP)est une maladie entérique du porc hautement contagieuse qui estcausée par le virus de la diarrhée épidémique porcine (PEDV).A?n de concevoir une méthode ELISA apte a`évaluer l’infection au PEDV et l’ef?cacitédes vaccins,on a élaboréet mis a`l’essai un test enzymatique indirect par immunoadsorption (iELISA)basésur une forme recombinante et tronquée de la protéine de spicule (S)expriméechez Escherichia coli .On a optimiséles paramètres de l’iELISA et établi la valeur seuil a`0,259en vertu d’une analyse des valeurs de DO de 80sérums a`PEDV négatif con?rmépar immunobuvardage de type western.Les tests de répétabilitéont révéléque les coef?cients de variation des sérums positifs intraessais et interessais se situaient tous deux sous la barre des 10%.Les essais de réactivitécroisée ont démontréque l’iELISA était spéci?que aux PEDV.Un test de neutralisation du virus d’après 7valeurs de DO a mis en évidence une corrélation positive entre les valeurs de DO et la neutralisation virale.Les résultats indiquent que cet iELISA est spéci?que,sensible etreproductible.Les analyses ultérieures devront s’attarder a`la relation entre les valeurs de DO des sérums et la neutralisation virale correspondante.[Traduit par la Rédaction]Mots-clés :ELISA indirect,PEDV,protéine S tronquée,neutralisation virale.IntroductionPorcine epidemic diarrhea virus (PEDV)is an envel-oped virus possessing an approximately 28kb,positive-sense,single-stranded RNA genome with a 5=cap and a 3=polyadenylated tail (Pensaert and Debouck 1978).The virus possesses glycosylated spike (S),Poll (P1),en-velope (E),glycosylated membrane (M),and an unglyco-sylated RNA-binding nucleocapsid (N)proteins (Song and Park 2012).The S protein of PEDV is a type I mem-brane glycoprotein composed of 1383amino acids and is predicted to contain a signal peptide (1–24aa),a large extracellular region,a single transmembrane domain (1334–1356aa),and a short cytoplasmic tail (Lee et al.2010).The S protein plays important roles in induction of neutralizing antibodies,speci?c receptor binding,and cell membrane fusion (Chang et al.2002;Sun et al.2008).The S1-GST protein (S1D,aa 636–789)is essential for in-duction of neutralizing antibodies (Sun et al.2007).The N-terminal region of the S protein is especially impor-tant for the receptor binding,and the 25–225aa region is indispensable to bind with amino peptidase N (receptor of PEDV)(Lee 2011).To date,most of the reported PEDV vaccines belong to attenuated vaccines and subunit vac-cines (Kweon et al.1999;Kang et al.2005;Song et al.2007;Received 8April 2015.Revision received 31July 2015.Accepted 4August 2015.Y.Li,F.Zheng,B.Fan,Y.Zou,and P.Jiang.Key Laboratory of Bacteriology,Ministry of Agriculture,College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,People’s Republic of China.H.M.Muhammad.Department of Epidemiology and Public Health,Faculty of Veterinary Science,University of Veterinary and Animal Sciences,Lahore,Pakistan.Correspondence author:Yufeng Li (e-mail:yufengli@/doc/597e2535d5bbfd0a785673aa.html ).811C a n . J . M i c r o b i o l .D o w n l o a d e d f r o m w w w .n r c r e s e a r c h p r e s s .c o m b y F o s h a n u n i v e r s i t y o n 11/03/15F o r p e r s o n a l u s e o n l y .Meng et al.2013).To date,M protein-based enzyme-linked immunosorbent assay (ELISA)and double anti-body sandwich ELISA have been developed to detect PEDV (Sozzi et al.2010;Ren etal.2011).N protein and viral protein have been used as coating antigens to estab-lish ELISA methods to detect antibodies against PEDV (Oh et al.2005;Hou et al.2007).Viral proteins have been used as a coating antigen of PEDV ELISA,and ELISA has been compared with virus neutralization.An overall agreement of 84.2%was generated between the serum neutralization and ELISA (Oh et al.2005).In the present study,an indirect ELISA (iELISA)based on a truncated recombinant protein S (tSc,N-terminal region 25–225aa of PEDV)was developed and assay conditions were optimized.The relationship between the iELISA results and virus neutralization titers was assessed.Materials and methodsSera and antibodyEighty-six sow sera were obtained from farms with the outbreaks of porcine epidemic diarrhea (PED)and health farms without clinical signs of PED.Among of them,6and 80sera were con?rmed as positive and negative,respectively,with western blot (WB)and immuno?uo-rescence (IFA)and were then stored at –80°C.The blood was drawn from the precaval vein of anesthetized pigs.Animal experiments were approved by the Institutional Animal Care and Ethics Committee of Nanjing Agricul-tural University (Approval No.IACECNAU20100902).Cloning and sequencing of the recombinant tSc protein geneThe tSc gene was ampli?ed from plasmids pFast-S (constructed by our laboratory)using a forward primer (5=-ATAGGATCCAGGTGCCAGTCTACT-3=)and a reverse primer (5=-GCGCTCGAGTTAAGGTTCATAGTAAAT-3=)(positions 20682–21284;DR13:GenBank accession No.JQ023162.1)containing restriction enzyme sites Bam HI and Xho I,underlined).The PCR product was cloned into the prokaryotic expression vector pET-28a(+)on corresponding restriction enzyme sites.This vector was designated as pET-28a/tSc,and its sequence was con-?rmed by sequencing analysis by Invitrogen (Shanghai,China).Expression and puri?cation of tSc proteinThe recombinant plasmid was transformed into Escherichia coli BL-21.The tSc protein was induced by addi-tion of 1mmol/L isopropyl-?-D -thiogalactopyranoside (IPTG).Four hours after IPTG induction,the cells were harvested and lysed by sonication.According to the man-ufacturer’s instructions,under the native condition,the recombinant protein was puri?ed by af?nity chro-matography with Ni-nitrilotriacetic acid agarose.The expressed protein was analyzed by sodium dodecyl sulfate –polyacrylamide gel electrophoresis (SDS–PAGE)with Coomassie brilliant blue R250and con?rmed by WBanalysis using His Tag monoclonal antibody (Boster,China)and PEDV polyclonal antibody prepared by immu-nizing rabbits with tSc protein that containing tSc.The puri?ed tSc protein was separated by SDS–PAGE using 12%separating gel and 5%stacking gel.Gels were stained with Coomassie brilliant blue R250,followed by immunoblotting.Brie?y,after transferring the proteins to nitrocellulose membrane,the membrane blocking was performed with 10%skimmed milk and incubated overnight at 4°C.The membranes were incubated with His Tag monoclonal antibody and PEDV polyclonal anti-body in blocking solution at 1:5000and 1:500dilution,respectively,for 2h at room temperature.After 1h of incubation,the membrane was extensively washed with PBS containing 0.05%Tween 20(PBST)and incubated for 45min with goat anti-mouse IgG (Boster,Wuhan).After washing,the membranes were incubated with horserad-ish peroxidase (HRP)-tagged goat anti-mouse IgG second-ary antibody (Boster,Wuhan),and the colorimetric reaction was developed using chemiluminescence lumi-nal reagents (Super Signal West Pico TrialKit,Pierce).iELISAA microtiter plate (96-well;Nunc,Nunclon,China)was coated with 100?L of tSc in 0.05mol/L bicarbonate–carbonate buffer (pH 9.6)and incubated for 2h at 37°C.Following 3washes with PBST,the plates were blocked for 2h at 37°C with 5%skimmed milk in PBST and then incubated for 1h at 37°C with 100?L serum samples in 5%skimmed milk in PBST.All samples were analyzed in triplicate.After5washes with PBST,the plates were fur-ther incubated with 100?L of HRP-conjugated protein A in PBST at 37°C.Then,the plates were washed again,and the colorimetric reaction was developed using 100?L of chromogenic substrates (0.4mol/L tetramethylbenzidine and 1mmol/L H 2O 2in 100mmol/L acetate buffer,pH 5.6)at 37°C.Color development was stopped with 2mol/L H 2SO 4,and the optical density at 450nm (OD 450)was recorded using an ELISA plate reader (BioTek,USA).Optimization of tSc iELISA working conditionsBased on the procedure described above,the optimal antigen concentration and sera dilutions were deter-mined through standard checkerboard titration proce-dures with triplicates.Brie?y,the tSc protein was coated on 96-well microtiter plates in serial 2-fold dilutions from 8?g to 250ng/well.Correspondingly,standard swine PEDV-positive serum and PEDV-negative control serum were also diluted in serial 2-fold dilutions from 1:25to 1:800for optimization.After the antigen and anti-serum dilutions were analyzed,HRP-labeled protein A was added to the plate with dilutions from 1:5000to1:20000to determine the optimal conjugate dilution.The conditions that gave the highest OD 450ratio between the positive and negative serum (P/N value)and an OD 450value for positive serum close to 1.0(with the OD 450value812Can.J.Microbiol.Vol.61,2015C a n . J . M i c r o b i o l .D o w n l o a d e d f r o m w w w .n r c r e s e a r c h p r e s s .c o m b y F o s h a n u n i v e r s i t y o n 11/03/15F o r p e r s o n a l u s e o n l y .of negative serum ≤0.2)were scored as optimal working conditions.After the conditions mentioned above were deter-mined,the coating conditions were optimized from at 37°C for 2h to 4°C for overnight.Then,the blocking buffers with 1%BSA,1%gelatin,5%and10%skimmed milk were used and blocked for 1,2,and 3h.The dilu-tions and incubation time of serum sample and HRP-conjugate staphylococal protein A were optimized with 15,30,45,60,90,or120min.The reactions were stopped and optimized by assessing 5,10,15,and 20min.Validation of tSc iELISATo establish a negative–positive cutoff value for this assay,80negative serum samples collected from healthy pig farms without clinical signs of PED (con?rmed by WB)were tested in duplicate using the tSc iELISA.The cutoff value of the ELISA was calculated from the mean OD values of the 80sera negative samples plus 2or 3standard deviations (SDs).This calculation gives 95%or 99%con? dence,respectively,that all negative values will fall within the de?ned range.To determine the speci?city of the tSc iELISA,positive sera against porcine circovirus type 2(PCV2),classical swine fever virus (CSFV),pseudorabies virus (PRV),foot-and-mouth disease virus (FMDV),porcine reproductive and respiratory syndrome virus (PRRSV),and transmissi-ble gastroenteritis virus (TGEV)were tested using the tSc iELISA protocol.Each sample was tested in triplicate,and the mean OD values were calculated.At the same time,6positive and another 30negative sera con?rmed by WB and IFA were detected by tSc iELISA to determine the sensitivity of this assay.Reproducibility experimentsEvaluation of assay reproducibility within and between runs was performed as previously proposed (Jacobson 1998).Six ?eld serum samples (5WB-positive samples and 1WB-negative sample)were selected for reproduc-ibility experiments.For intra-assay reproducibility,3replicates of each serum sample were assigned to the same plate.For inter-assay (between-run)reproducibil-ity,3replicates of each sample were run on different plates.The mean OD values,standard deviation (SD),and coef?cient of variation (CV)were calculated.Neutralization testVero-E6cells (1×105cells/well)were seeded in a 96-well cell culture plate,and plates were incubated overnight at 37°C with 5%CO 2until a con?uent monolayer formed.Seven serum samples with different OD values (1.531,1.294,0.980,0.784,0.661,0.378,and 0.078)were heated at 56°C for 30min and used for testing.Sera were diluted 2-fold and incubated with 200TCID 50of strain DR13at 37°C for 1h.After incubation,100?L of the serum+virus mixture was transferred from each well of the incuba-tion plate to a 96-well cell culture plate containing Vero-E6cells.After 1h of incubation at 37°C,the serum+virus mixtures were replaced by complete Dul-becco’s Modi?ed Eagle Medium with 10%fetal calf se-rum.The plates were incubated at 37°C with 5%CO 2for 5days,and the numbers of wells with cells with cyto-pathic effect were counted under an inverted micro-scope and neutralization titers were evaluated.StatisticsPASW Statistics for Windows,Version 18(SPSS Inc.,Chicago,Illinois,USA),was used to analyze standard de-viation,CV,and correlation analysis.ResultsExpression,puri?cation,and identi?cation of tSc proteinSDS–PAGE and Coomassie brilliant blue staining dem-onstrated the successful expression of the tSc protein in E.coli (Fig.1a ).Compared with the expression of induced E.coli /pET-28a and E.coli culture,tSc with a molecular mass of approximately 28kDa was observed in induced E.coli /pET-28a-Sc (Fig.1a ).The tSc protein was expressed in large amounts and puri?ed using nickel af?nity chro-matography.SDS–PAGE indicated that the protein was of high purity (Fig.1a ).In addition,WB showed that the tSc protein could be detected with an anti-His antibody and PEDV-positive sera (Fig.1b ). Fig.1.Identi?cation of the truncated recombinant protein S (tSc)protein by SDS–PAGE (a )and western blot (b )by His tag monoclonal antibody and porcine epidemic diarrhea virus (PEDV)polyclonal antibody.(a )Lanes:M,unstained protein molecular mass marker;1,pET-28a-Sc induced with IPTG;2,pET-28a-Sc not induced with IPTG;3,supernatant of cell lysates;4,precipitation of cell lysates;5,puri?ed Sc protein.(b )Cell lysates were incubated with His tag monoclonal antibody (lanes 1and 2)and PEDV polyclonal antibody (lanes 3and 4).Lanes:1and 3,pET-28a-Sc induced with IPTG;2and 4,pET-28a-Sc not induced withIPTG.Li et al.813C a n . J . M i c r o b i o l .D o w n l o a d e d f r o m w w w .n r c r e s e a r c h p r e s s .c o m b y F o s h a n u n i v e r s i t y o n 11/03/15F o r p e r s o n a l u s e o n l y .Optimization of iELISA working conditionsUsing a checkerboard ELISA,the optimal antigen concentration and serum sample dilution were set at 1?g/well and 1:100,respectively,based on the following standards:the OD 450value of positive serum was close to 1.0,the OD 450ratio between positive and negative serum (P/N value)was highest,and the background was /doc/597e2535d5bbfd0a785673aa.html ing the same standards,the optimal blocking condi-tion was at 37°C for 2h,and the optimal dilution of the conjugate was de?ned as 1:20000.After the above-mentioned conditions were determined,it was found that 5%skimmed milk in PBST was the optimal block-ing buffer,and the best blocking time was 2h at 37°C.For the optimal exposure time of serum samples and conjugate,it was determined that incubations of 60and 45min for the serum samples and the conjugate,respectively,were suf?cient and time-saving in the tSc ELISA,as the P/N value was relatively stable and high.Finally,the optimal stopping time was determined to be 15min (Fig.2).Cutoff value of iELISAThe OD values of 80negative serum samples varied from a minimum of 0.052to a maximum of 0.201.Thus,samples with OD values of ≤0.223were considered neg-ative,and those with values of≥0.259were considered positive (Supplemental Table 11).After the cutoff was determined,the speci?city of the tSc iELISA was evaluated by testing the reactivity of antibodies against other porcine viruses.The OD values of standard positive sera againstPCV2,CSFV,PRV,FMDV,TGEV,and PRRSV are shown in Table 1.The OD values of all anti-sera were smaller than the cutoff value.These data revealed that there is no cross-reactivity between the PEDV tSc antigen and antibodies against other por-cine viruses,proving that the tSc antigen is speci?c for antibodies against PEDV.Diagnostic sensitivity tests showed that 6positive sera and another 30negative sera identi?ed by WB and IFA were still positive and negative in ELISA results.Repeatability of the tSc iELISAThe repeatability of tSc iELISA was determined by comparing the OD value of each ?eld serum sample.As shown in Table 2,the intra-assay CV of 5positive serum samples ranged from 0.019to0.094,whereas the inter-assay CV of these positive serum samples ranged be-tween 0.038and 0.088.These data indicate that the tSc iELISA is repeatable and yields low and acceptable varia-tion (DeSilva et al.2003).Neutralization testsThe results showed that 6positive sera had different levels of virus neutralization activity.Interestingly,OD values are positively correlated with virus neutralization activity (Fig.3).More positive sera are needed to further con?rm the relationship between OD values and virus neutralization titers.DiscussionIn China,PEDV was ?rst con?rmed in 1984,and since 2010,it has caused enteric disease with a devastat-ing impact on swine industry.This disease is character-ized by high morbidity and mortality in preweaning piglets,causing serious economic losses to the swine industry in China (Gao et al.2013;Li et al.2014).On the basis of the gene sequence of PEDV,many methods had been developed to detect the infection of PEDV,such as RT-PCR (Ishikawa et al.1997),duplex RT-PCR (Kim et al.2001),immunohistochemistry,in situ hy-bridization (Kim and Chae 2002),and RT-LAMP (Ren and Li 2011).Recombinant N protein had been used to establish an ELISA method to detect antibodies against PEDV (Hou et al.2007).To date,the recombi-nant S protein as a coating antigen for the ELISA method has not been reported.1Supplementary data are available with the article through the journal Web site at /doc/597e2535d5bbfd0a785673aa.html /doi/suppl/10.1139/cjm-2015-0213.Table 1.Speci?city of the truncated S pro-tein indirect ELISA to antibodies against some swine viruses.Virus antisera X ¯±SD PEDV1.037±0.012PRV 0.054±0.030PCV20.046±0.016FMDV 0.066±0.028PRRSV 0.055±0.035CSFV 0.047±0.023TGEV0.086±0.013Negative serum0.063±0.008Note:Data are the mean ±standard deviation of 3replicate measurements.PEDV,porcine epi-demic diarrhea virus;PRV,pseudorabies virus;PCV2,positive sera against porcine circovirus type2;FMDV,foot-and-mouth disease virus;PRRSV,porcine reproductive and respiratory syndrome vi-rus;CSFV,classical swine fever virus;TGEV,trans-missible gastroenteritis virus.Table 2.Intra-assay and inter-assay repeatability of the in-direct ELISA.Serum sample Intra-assay variability Inter-assay variability X¯±SD CV X¯±SD CV 1 1.095±0.0210.019 1.087±0.0510.0472 1.066±0.0770.0720.987±0.0560.0573 1.076±0.0750.070 1.100±0.0970.08840.955±0.0560.0590.912±0.0350.03850.730±0.0640.0880.690±0.0660.096Negative0.096±0.0090.0940.100±0.0090.090Note:Data are the mean ±standard deviation of 3replications.Li et al.815C a n . J . M i c r o b i o l .D o w n l o a d e d f r o m w w w .n r c r e s e a r c h p r e s s .c o m b y F o s h a n u n i v e r s i t y o n 11/03/15F o r p e r s o n a l u s e o n l y .Recent studies reported that the N-terminal region (25–225amino acids)of PEDV is associated with the binding of the virus to the receptor aminopeptidase N (Lee 2011).So,we postulated that antibody against this protein fragment may be associated with virus neu-tralization.In this study,the tSc protein (25–225ami-no acids)was expressed and an iELISA method was constructed.Cross-reactivity assays revealed that the tSc iELISA was PEDV-speci?c,and repeatability tests demonstrated that the assay is repeatable.Thus,the tSc iELISA is simpler to produce and perform,time-saving,and may be more suitable for large-scale surveys of PEDV /doc/597e2535d5bbfd0a785673aa.html bining virus neutral-ization results with 7different OD values sera,we ?nd that the OD values obtained by iELISA established by tSc are positively correlated with virus neutralization.The preliminary results showed that the sensitivity of this assay is 100%;however,more positive sera samples should be used to further con?rm the sensitivity.ConclusionThe tSc iELISA established in the present study was repeatable and speci?c for PEDV antibody detection,simple and economical to produce and perform,and time-saving.The present report may facilitate the devel-opment of a reliable tool for the large-scale detection of PEDV neutralization antibodies and assess the effective-ness of vaccines.AcknowledgementsWe thank Jiang Ping for helpful comments.The au-thors declare no potential con?icts of interest with re-spect to the research,authorship,and (or)publication of this article.This study was supported by public welfare grants from the Ministry of Agriculture,the People’s Re-public of China (201203039),and Priority Academic Pro-gram Development (PAPD)of Jiangsu Higher Education Institutions. ReferencesChang,S.H.,Bae,J.L.,Kang,T.J.,Kim,J.,Chung,G.H.,Lim,C.W.,et al.2002.Identi?cation of the epitope region capable of inducing neutralizing antibodies against the porcine epi-demic diarrheavirus.Mol.Cells,14(2):295–299.PMID:12442904.DeSilva,B.,Smith,W.,Weiner,R.,Kelley,M.,Smolec,J.,Lee,B.,et al.2003.Recommendations for the bioanalytical method validation of ligand-binding assays to support pharmacoki-netic assessments of macromolecules.Pharm.Res.20(11):1885–1900.doi:10.1023/B:PHAM.0000003390.51761.3d .PMID:14661937.Gao,Y.,Kou,Q.,Ge,X.,Zhou,L.,Guo,X.,and Yang,H.2013.Phylogenetic analysis of porcine epidemic diarrhea virus ?eld strains prevailing recently in China.Arch.Virol.158(3):711–715.doi:10.1007/s00705-012-1541-2.PMID:23151819.Hou,X.L.,Yu,L.Y.,and Liu,J.2007.Development and evaluation of enzyme-linked immunosorbent assay based on recombi-nant nucleocapsid protein for detection of porcine epidemic diarrhea (PEDV)antibodies.Vet.Microbiol.123(1–3):86–92.doi:10.1016/j.vetmic.2007.02.014.PMID:17368968.Ishikawa,K.,Sekiguchi,H.,Ogino,T.,and Suzuki,S.1997.Direct and rapid detection of porcine epidemic diarrhea virus by RT-PCR.J.Virol.Methods,69(1–2):191–195.doi:10.1016/S0166-0934(97)00157-2.PMID:9504764.Jacobson,R.H.1998.Validation of serological assays for diagno-sis of infectious diseases.Rev.Sci.Tech.17(2):469–526.PMID:9713892.Kang,T.J.,Kim,Y.S.,Jang,Y.S.,and Yang,M.S.2005.Expression of the synthetic neutralizing epitope gene of porcine epi-demic diarrhea virus in tobacco plants without nicotine.Vac-cine,23(17–18):2294–2297.doi:10.1016/j.vaccine.2005.01.027.PMID:15755614.Kim,O.,and Chae,/doc/597e2535d5bbfd0a785673aa.html parison of reverse transcription polymerase chain reaction,immunohistochemistry,and in situ hybridization for the detection of porcine epidemic di-arrhea virus in pigs.Can.J.Vet.Res.66(2):112–116.PMID:11989732.Kim,S.Y.,Song,D.S.,and Park,B.K.2001.Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR.J.Vet.Diagn.Invest.13(6):516–520.doi:10.1177/104063870101300611.PMID:11724144.Kweon,C.H.,Kwon,B.J.,Lee,J.G.,Kwon,G.O.,and Kang,Y.B.1999.Derivation of attenuated porcine epidemic diarrheaFig.3.Virus neutralization titers and optical density (OD)values of truncated recombinant protein S indirect enzyme-linked immunosorbent assay for 7sera.816Can.J.Microbiol.Vol.61,2015C a n . J . M i c r o b i o l .D o w n l o a d e d f r o m w w w .n r c r e s e a r c h p r e s s .c o m b y F o s h a n u n i v e r s i t y o n 11/03/15F o r p e r s o n a l u s e o n l y .virus (PEDV)as vaccine candidate.Vaccine,17(20–21):2546–2553.doi:10.1016/S0264-410X(99)00059-6.PMID:10418901.Lee,D.K.2011.The N-terminal region of the porcine epidemic diarrhea virus spike protein is important for the receptor binding.Korean J.Microbiol.Biotechnol.39(2):140–145.Lee,D.K.,Park,C.K.,Kim,S.H.,and Lee,C.2010.Heterogeneity in spike protein genes of porcine epidemic diarrhea viruses isolated in Korea.Virus Res.149(2):175–182.doi:10.1016/j.virusres.2010.01.015.PMID:20132850.Li,R.,Qiao,S.,Yang,Y.,Su,Y.,Zhao,P.,Zhou,E.,and Zhang,G.2014.Phylogenetic analysis of porcine epidemic diarrhea vi-rus (PEDV)?eld strains in central China based on the ORF3gene and the main neutralization epitopes.Arch.Virol.159:1057–1065.doi:10.1007/s00705-013-1929-7.PMID:24292967.Meng,F.,Ren,Y.,Suo,S.,Sun,X.,Li,X.,Li,P.,et al.2013.Evalu-ation on the ef?cacy and immunogenicity of recombinant DNA plasmids expressing spike genes from porcine trans-missible gastroenteritis virus and porcine epidemic diar-rhea virus.PLoSOne,8(3):e57468.doi:10.1371/journal.pone.0057468.PMID:23526943.Oh,J.S.,Song,D.S.,Yang,J.S.,Song,J.Y.,Moon,H.J.,Kim,T.Y.,and Park,/doc/597e2535d5bbfd0a785673aa.html parison of an enzyme-linked immu-nosorbent assay with serum neutralization test for serodiag-nosis of porcine epidemic diarrhea virus infection.J.Vet.Sci.6(4):349–352.PMID:16294000.Pensaert,M.,and Debouck,P.1978.A new coronavirus-like par-ticle associated with diarrhea in swine.Arch.Virol.58:243–247.doi:10.1007/BF01317606.PMID:83132.Ren,X.,and Li,P.2011.Development of reverse transcription loop-mediated isothermal ampli?cation for rapid detection of porcine epidemic diarrhea virus.Virus Genes,42(2):229–235.doi:10.1007/s11262-011-0570-3.PMID:21286798. Ren,X.,Suo,S.,and Jang,Y.S.2011.Development of a porcine epidemic diarrhea virus M protein-based ELISA for virus de-tection.Biotechnol.Lett.33(2):215–220.doi:10.1007/s10529-010-0420-8.PMID:20882317.Song,D.,and Park,B.2012.Porcine epidemic diarrhoea virus:a comprehensive review of molecular epidemiology,diagno-sis,and vaccines.Virus Genes,44(2):167–175.doi:10.1007/s11262-012-0713-1.PMID:22270324.Song,D.S.,Oh,J.S.,Kang,B.K.,Yang,J.S.,Moon,H.J.,Yoo,H.S.,et al.2007.Oral ef?cacy of Vero cell attenuated porcine epi-demic diarrhea virus DR13strain.Res.Vet.Sci.82(1):134–140.doi:10.1016/j.rvsc.2006.03.007.PMID:16730762.Sozzi,E.,Luppi,A.,Lelli,D.,Martin,A.M.,Canelli,E.,Brocchi,E.,et /doc/597e2535d5bbfd0a785673aa.html parison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diar-rhoea virus.Res.Vet.Sci.88(1):166–168.doi:10.1016/j.rvsc.2009.05.009.PMID:19501378.Sun,D.B.,Feng,L.,Shi,H.Y.,Chen,J.F.,Liu,S.W.,Chen,H.Y.,and Wang,Y.F.2007.Spike protein region (aa 636789)of porcine epidemic diarrhea virus is essential for induction of neutral-izing antibodies.Acta Virol.51(3):149–156.PMID:18076304.Sun,D.,Feng,L.,Shi,H.,Chen,J.,Cui,X.,Chen,H.,et al.2008.Identi?cation of 2novel B cell epitopes on porcine epidemic diarrhea virus spike protein.Vet.Microbiol.131(1–2):73–81.doi:10.1016/j.vetmic.2008.02.022.PMID:18400422.Li et al.817C a n . J . M i c r o b i o l .D o w n l o a d e d f r o m w w w .n r c r e s e a r c h p r e s s .c o m b y F o s h a n u n i v e r s i t y o n 11/03/15F o r p e r s o n a l u s e o n l y .。
〈87〉 Biological Reactivity Tests, In Vitro
á87ñ BIOLOGICAL REACTIVITY TESTS, IN VITROThe following tests are designed to determine the biological reactivity of mammalian cell cultures following contact with the elastomeric plastics and other polymeric materials with direct or indirect patient contact or of specific extracts prepared from the materials under test. It is essential that the tests be performed on the specified surface area. When the surface area of the specimen cannot be determined, use 0.1 g of elastomer or 0.2 g of plastic or other material for every mL of extraction fluid.Exercise care in the preparation of the materials to prevent contamination with microorganisms and other foreign matter. Three tests are described (i.e., the Agar Diffusion Tes t, the Direct Contact Tes t, and the Elution Tes t).1 The decision as to which type of test or the number of tests to be performed to assess the potential biological response of a specific sample or extract depends upon the material, the final product, and its intended use. Other factors that may also affect the suitability of a sample for a specific use are the polymeric composition; processing and cleaning procedures; contacting media; inks;adhesives; absorption, adsorption, and permeability of preservatives; and conditions of storage. Evaluation of such factors should be made by appropriate additional specific tests before determining that a product made from a specific material is suitable for its intended use. Materials that fail the in vitr o tests are candidates for the in viv o tests described in Biological Reactivity Tests, In Vivo á88ñ.PROCEDURES•T EST C ONTROLPositive control:Polyurethane film containing zinc diethyldithiocarbamate (ZDEC)2 or zinc dibutyldithiocarbamate (ZDBC) Cell culture preparation:Prepare multiple cultures of L-929 (ATCC cell line CCL 1, NCTC clone 929; alternative cell lines obtained from a standard repository may be used with suitable validation) mammalian fibroblast cells inserum-supplemented minimum essential medium having a seeding density of about 105 cells per mL. Incubate the cultures at 37 ± 1° in a humidified incubator for NLT 24 h in a 5 ± 1% carbon dioxide atmosphere until a monolayer, with greater than 80% confluence, is obtained. Examine the prepared cultures under a microscope to ensure uniform, near-confluent monolayers. [N OTE—The reproducibility of the in vitro biological reactivity tests depends upon obtaining uniform cell culture density.]Extraction solvents:Sodium Chloride Injectio n [see monograph—use Sodium Chloride Injectio n containing 0.9% of sodium chloride (NaCl)]. Alternatively, serum-free mammalian cell culture media or serum-supplemented mammalian cell culture media may be used. Serum supplementation is used when extraction is done at 37° for 24 h.•A PPARATUSAutoclave:Employ an autoclave capable of maintaining a temperature of 121 ± 2°, equipped with a thermometer, a pressure gauge, a vent cock, a rack adequate to accommodate the test containers above the water level, and a water cooling system that will allow for cooling of the test containers to about 20°, but not below 20°, immediately following the heating cycle. Oven:Use an oven, preferably a mechanical convection model, that will maintain operating temperatures in the range of 50°–70° within ±2°.Incubator:Use an incubator capable of maintaining a temperature of 37 ± 1° and a humidified atmosphere of 5 ± 1% carbon dioxide in air.Extraction containers:Use only containers, such as ampuls or screw-cap culture test tubes, or their equivalent, of Type I glass. If used, culture test tubes, or their equivalent, are closed with a screw cap having a suitable elastomeric liner. The exposed surface of the elastomeric liner is completely protected with an inert solid disk 50–75 µm in thickness. A suitable disk can be fabricated from polytef.Preparation of apparatus:Cleanse all glassware thoroughly with chromic acid cleansing mixture and, if necessary, with hot nitric acid followed by prolonged rinsing with Sterile Water for Injectio n. Sterilize and dry by a suitable process for containers and devices used for extraction, transfer, or administration of test material. If ethylene oxide is used as the sterilizing agent, allow NLT 48 h for complete degassing.•P ROCEDUREPreparation of sample for extracts:Prepare as directed in the Procedur e in á88ñ.Preparation of extracts:Prepare as directed for Preparation of extract s in á88ñ using either Sodium Chloride Injectio n [0.9% sodium chloride (NaCl)] or serum-free mammalian cell culture media as Extraction solvent s. [N OTE—If extraction is done at 37° for 24 h in an incubator, use cell culture media supplemented by serum. The extraction conditions should not in any instance cause physical changes, such as fusion or melting of the material pieces, other than a slight adherence.]•A GAR D IFFUSION T ESTThis test is designed for elastomeric closures in a variety of shapes. The agar layer acts as a cushion to protect the cells from mechanical damage while allowing the diffusion of leachable chemicals from the polymeric specimens. Extracts of materials that are to be tested are applied to a piece of filter paper.Sample preparation:Use extracts prepared as directed, or use portions of the test specimens having flat surfaces NLT 100 mm2 in surface area.Positive control preparation:Proceed as directed for Sample preparatio n.Negative control preparation:Proceed as directed for Sample preparatio n.Procedure:Using 7 mL of cell suspension prepared as directed in Cell culture preparatio n, prepare the monolayers in plates having a 60-mm diameter. Following incubation, aspirate the culture medium from the monolayers, and replace it with serum-supplemented culture medium containing NMT 2% of agar. [N OTE—The quality of the agar must be adequate to1Further details are given in the following publications of the American Society for Testing and Materials, 1916 Race St., Philadelphia, PA 19103: Standard test method for agar diffusion cell culture screening for cytotoxicity, ASTM Designation F 895-84; Standard practice for direct contact cell culture evaluation of materials for medical devices, ASTM Designation F 813-83.2ZDEC and ZDBC polyurethanes are available from the Food and Drug Safety Center, Hatano Research Institute, Ochiai 729–5, Hadanoshi, Kanagawa 257, Japan.support cell growth. The agar layer must be thin enough to permit diffusion of leached chemicals.] Place the flat surfaces of Sample preparatio n, Positive control preparatio n, and Negative control preparatio n or their extracts in an appropriate extracting medium, in duplicate cultures in contact with the solidified agar surface. Use no more than three specimens per prepared plate. Incubate all cultures for NLT 24 h at 37 ± 1°, preferably in a humidified incubator containing 5 ± 1% of carbon dioxide. Examine each culture around each sample, negative control, and positive control under a microscope, using a suitable stain, if desired.Interpretation of results:The biological reactivity (cellular degeneration and malformation) is described and rated on a scale of 0–4 (see Table 1). Measure the responses of the cell cultures to the Sample preparatio n, the Positive control preparatio n, and the Negative control preparatio n. The cell culture test system is suitable if the observed responses to the Negative control preparatio n is grade 0 (no reactivity) and to the Positive control preparatio n is at least grade 3 (moderate).The sample meets the requirements of the test if the response to the Sample preparatio n is not greater than grade 2 (mildly reactive). Repeat the procedure if the suitability of the system is not confirmed.Table 1. Reactivity Grades for Agar Diffusion Test and Direct Contact TestGrade Reactivity Description of Reactivity Zone0None No detectable zone around or under specimen1Slight Some malformed or degenerated cells under specimen2Mild Zone limited to area under specimen and less than 0.45 cm beyond specimen3Moderate Zone extends 0.45–1.0 cm beyond specimen4Severe Zone extends greater than 1.0 cm beyond specimen•D IRECT C ONTACT T ESTThis test is designed for materials in a variety of shapes. The procedure allows for simultaneous extraction and testing of leachable chemicals from the specimen with a serum-supplemented medium. The procedure is not appropriate for very low- or high-density materials that could cause mechanical damage to the cells.Sample preparation:Use portions of the test specimen having flat surfaces NLT 100 mm2 in surface area.Positive control preparation:Proceed as directed for Sample preparatio n.Negative control preparation:Proceed as directed for Sample preparatio n.Procedure:Using 2 mL of cell suspension prepared as directed in Cell culture preparatio n, prepare the monolayers in plates having a 35-mm diameter. Following incubation, aspirate the culture medium from the cultures, and replace it with 0.8 mL of fresh culture medium. Place a single Sample preparatio n, a Positive control preparatio n, and a Negative controlpreparatio n in each of the duplicate cultures. Incubate all cultures for NLT 24 h at 37 ± 1° in a humidified incubator containing 5 ± 1% of carbon dioxide. Examine each culture around each Sample preparatio n, a Positive controlpreparatio n, and a Negative control preparatio n, under a microscope, using a suitable stain, if desired.Interpretation of results:Proceed as directed for Interpretation of result s in Agar Diffusion Tes t. The sample meets the requirements of the test if the response to the Sample preparatio n is not greater than grade 2 (mildly reactive). Repeat the procedure if the suitability of the system is not confirmed.•E LUTION T ESTThis test is designed for the evaluation of extracts of polymeric materials. The procedure allows for extraction of the specimens at physiological or nonphysiological temperatures for varying time intervals. It is appropriate for high-density materials and for dose-response evaluations.Sample preparation:Prepare as directed in Preparation of extract s, using either Sodium Chloride Injectio n [0.9% sodium chloride (NaCl)] or serum-free mammalian cell culture media as Extraction solvent s. If the size of the sample cannot be readily measured, a mass of NLT 0.1 g of elastomeric material or 0.2 g of plastic or polymeric material per mL of extraction medium may be used. Alternatively, use serum-supplemented mammalian cell culture media as the extracting medium to simulate more closely physiological conditions. Prepare the extracts by heating for 24 h in an incubator containing 5± 1% of carbon dioxide. Maintain the extraction temperature at 37 ± 1°, because higher temperatures may cause denaturation of serum proteins.Positive control preparation:Proceed as directed for Sample preparatio n.Negative control preparation:Proceed as directed for Sample preparatio n.Procedure:Using 2 mL of cell suspension prepared as directed in Cell culture preparatio n, prepare the monolayers in plates having a 35-mm diameter. Following incubation, aspirate the culture medium from the monolayers, and replace it with extracts of the Sample preparatio n, Positive control preparatio n, or Negative control preparatio n. The serum-supplemented and serum-free cell culture media extracts are tested in duplicate without dilution (100%). The Sodium Chloride Injectio n extract is diluted with serum-supplemented cell culture medium and tested in duplicate at 25% extract concentration.Incubate all cultures for 48 h at 37 ± 1° in a humidified incubator preferably containing 5 ± 1% of carbon dioxide. Examine each culture at 48 h, under a microscope, using a suitable stain, if desired.Interpretation of results:Proceed as directed for Interpretation of result s in Agar Diffusion Tes t but use Table 2. The sample meets the requirements of the test if the response to the Sample preparatio n is not greater than grade 2 (mildly reactive).Repeat the procedure if the suitability of the system is not confirmed. For dose-response evaluations, repeat the procedure, using quantitative dilutions of the sample extract.1Slight Less than or equal to 20% of the cells are round, loosely attached, and without intracytoplasmic granules; occasional lysed cells are present 2Mild Greater than 20% to less than or equal to 50% of the cells are round and devoid of intracyto-plasmic granules; no extensive cell lysis and empty areas between cells 3Moderate Greater than 50% to less than 70% of the cell layers contain rounded cells or are lysed 4Severe Nearly complete destruction of the cell layers ADDITIONAL REQUIREMENTS •USP R EFERENCE S TANDARDS á11ñUSP High-Density Polyethylene RS (Negative Control)Table 2. Reactivity Grades for Elution TestGradeReactivity Conditions of All Cultures 0None Discrete intracytoplasmic granules; no cell lysis。
Primary+School+English+Comparative+Level
Forms of comparative senses
Forms of the comparative degree: The comparative degree of an adjective is usually constructed by adding "- er" after it. For example, the comparative level of "happiness" is "happiness".
The basic form and usage of comparative level
Basic form
The test form of the comparative level mainly includes listening, reading, writing, and speaking Each part has multiple tasks, which are designed to test students' language ability in different aspects
VS
Usage
The comparative level is used to evaluate students' English language ability, and can also be used as a reference for students to evaluate their own English ability
通过iSpring快速制作检测试题
通过iSpring快速制作测试题使用iSpring QuizMaker ——一款容易操作的、高级的测验创建软件,可以在几分钟内创建出吸引人的测验、考试和评估测试。
在eLearning中了解学习者理解知识的程度、通问卷调查来收集顾客的反馈信息或者只是为了兴趣而创建测验,使用这款全新的具有先进功能的iSpring QuizMaker可以很容易达到这些目的。
用iSpring软件创建的测验可以并入eLearning课件中,或者嵌入到网页中,又或者上传和追踪到任何一个符合的SCORM/AICC标准的学习管理系统(LMS)上或iSpring Online的学习管理系统(LMS)上。
给企业和eLearning设计行之有效的测验不管你是创建测验的行家还是新手,通过使用iSpring QuizMaker,你都会发现创建测验和调查是多么简单的一件事。
QuizMaker具有强大的创建测验的功能,您可以通过一个超级简单的办公类型界面进入来体验这些功能。
创建评分型测验和考试或者调查和评估测试的23种问题题型。
∙使评分型问题和调查型问题相混合∙给测验问题附上图片、音频和视频∙通过添加方程式和公式创建综合性数学考试题∙在问题与问题间添加含有补充信息的幻灯片管理和自定义测验打乱答案顺序,并随机从问题库里选择问题,以保证每个测试者有不同的测试题。
你可以预览问题,也可以预览整个测验来即时检查你的工作。
∙根据用户的回答组织分支结构情景∙为每一个问题设置以结果为基础的自定义反馈信息∙使问题和答案随机化∙整理问题放入问题库∙控制测试时间和测试次数∙快速浏览一个问题或整个测试∙自定义文本标签和选择播放器配色方案∙将测验题打印出来以便用于纸质测试∙为每一个测试制作独立的Flash(.SWF)动画∙通过邮件、LMS或者网络服务器跟踪结果∙仅通过点击鼠标即可将测试上传至iSpring Online的学习管理系统(LMS)参与23种测试题型并从中获得乐趣有了iSpring,把测试和丰富多彩的活动联系起来就变得如同填写一张表格那么容易了。
pat (basic level) practice
pat (basic level) practicePAT(Programming Ability Test)是浙江大学计算机科学与技术学院编写的一个面向大学生的程序设计能力考试,分为Basic、Advanced 和Top 三个等级,其中Basic Level 是入门级别的考试,主要考察基础的编程语法和算法知识。
以下是PAT Basic Level 中的一些练习题目:1001 A+B Format题目描述:给定两个整数 A 和B,输出A+B 的和,并按照每三位数字一组的方式输出,其中以逗号分隔。
输入格式:每个输入文件包含一组测试数据,每组数据占一行,包含两个整数A 和B,中间以空格分隔。
其中,-10^6≤A,B≤10^6,A 和B 不全为0。
输出格式:对于每个测试用例,输出A+B 的和,并按照每三位数字一组的方式输出,其中以逗号分隔,不能有多余的空格。
样例输入:-1000000 9样例输出:-999,9911002 写出这个数题目描述:给定一个不超过1000 的正整数N,要求将其每个位上的数字从左到右按照中文读写的方式输出。
输入格式:每个输入文件包含一组测试数据,每组数据占一行,包含一个不超过1000 的正整数N。
输出格式:对于每个测试用例,在一行中输出N 的每个位上数字的中文读音,相邻数字间以“空格”分隔,但输出结尾不能有空格。
样例输入:1234567890987654321123456789样例输出:yi er san si wu liu qi ba jiu ling jiu ba qi liu wu si san er yi1003 我要通过!题目描述:给定一个只包含字符'P'、'A'、'T'、'e'、's'、't' 的字符串S,本题要求你判断它是否能够正确地打出一行程序代码:即正确的拼写出字符串"PATest"。
Edexcel INSET培训07-08技术支持指南说明书
Assessment Criteria Unit 6: Technical SupportCandidate 6956Strand aMark Band 1 Mark Band 2 Mark Band 3The learner:•successfully installs one hardware and one software upgrade, with extensive prompting •carries out some limited testing of the system, but not sufficient to guarantee that it works correctly.Whilst working on the upgrade, thelearner adheres to relevant standard wayof working, but needs frequent prompting. The learner:•successfully installs one hardware and onesoftware upgrade, with only limited prompting• carries out adequate testing of the system toensure that it works correctly.Whilst working on the upgrade, the learneradheres to relevant standard way ofworking, with only occasional prompting.The learner:•successfully installs one hardware and onesoftware upgrade, independently• carries out extensive testing of the systemto ensure that it works correctly and tooptimise its performance.Whilst working on the upgrade, the learneradheres to relevant standard way ofworking, independently.(0 — 5)(6 — 8)(9 — 10) Comments: The candidate has provided evidence of successfully installing one item of software (WinCleaner One Click) and installing additional RAM, there is, however, no check for compatibility of the new RAM with the current system. The evidence is well annotated through a pdf presentation with notes and the upgrades are shown to be working. There is ample evidence of testing the software upgrade and the evidence clearly indicates it has been successfully installed. The candidate has produced a test plan for the hardware update, thoroughly tested the newly installed software and produced a rationale of why the upgrades were undertaken. There is a brief comment to suggest that the upgrades were carried out on the system built in unit 4. There should be some indication of this being undertaken (page 101 of the unit specification).Good photographic evidence of installing the additional RAM has been produced. There is clear reference to working safely; the candidate has commented regarding isolating the computer from the mains and use of anti-static wrist band. The testing is thorough and the confirmation of SWOW allows this candidate to attain marks in mark band 2. More explicit evidence of optimising performance would have placed this candidate in mark band 3.Mark awarded 8Strand bMark Band 1 Mark Band 2 Mark Band 3The learner produces an on-screen technical support manual that gives brief instructions for carrying out some routine maintenance and troubleshooting, but omits some essential information. The learner produces a clearly presented, on-screen technical support manual that givesdetailed instructions for carrying out routinemaintenance and troubleshooting.The learner produces a clearly presented, on-screen technical support manual that givescomplete and easy to follow instructions forcarrying out routine maintenance andtroubleshooting, enabling someone else tomaintain the system without furtherassistance.(0 - 8)(9 - 12)(13 - 15)Comments: The candidate has produced a well presented and detailed technical manual which is in a format suitable for on-screen display. However, the manual needs to be easy to read on screen which should involve good navigation so that the reader can locate the information required quickly and easily. Whilst there is a link to each major section and it contains sufficient information relating to routine maintenance i.e. Defrag, backup and restore, the manual does not have an index to all the topics without having to explore each section to see what they contain. Trouble shooting is also included together with suggestions of the less obvious solutions to routine maintenance which might be required to be undertaken. The candidate has attempted to include many of the sections indicated in 6.1 and 6.2 of the unit specification.Each item is introduced and for many of the routines there are screen shots which help the reader follow the instructions. There is a maintenance schedule included together with internet connectivity and security issues.The candidate has presented a manual which can be read on screen with sufficient audience awareness to meet the requirements of the top of Mark Band 2.Although the manual is well presented, to access mark band 3, the reader must be able to locate the information required quickly and easily and there should be a recommended procedure for recording any maintenance work carried out. The candidate has suggested that all work undertaken be recorded but has not produced any details relating to a procedure for recording this work.Mark Awarded 12.Strand cMark Band 1 Mark Band 2 Mark Band 3The presentation includes:• a brief description of the key features of at least four web-based tools for collaborativeworking• a description of the capabilities andlimitations of each tool, but with little or nocomparison• a demonstration of aspects of the setup and use of a web-based tool, but not detailedenough to give a clear picture of what isinvolved. The presentation includes:• a detailed description — supported byexamples — of the key features of at leastfour web-based tools for collaborative working• a detailed description and comparison of thecapabilities and limitations of each tool• a clear demonstration of the setup and use ofa web-based tool, giving an accurate pictureof what is involved.The presentation includes:• a comprehensive description — supportedby a range of well-chosen examples — ofthe key features of at least four web-basedtools for• collaborative working• a detailed description and comparison ofthe capabilities and limitations of each tool,assessing their suitability for particulartasks•an effective demonstration of the setup anduse of a web-based tool, giving a full andaccurate picture of what is involved.(0 - 8)(9 - 11)(12 - 15)Comments: A series of good explanations with comparisons of four tools used for collaborative working with well chosen examples and comprehensive descriptions of the capabilities and limitations of each tool, assessing their suitability for particular tasks. Setting up and managing a ‘Sharepoint’ site has been fully and carefully explained. The candidate has used a range of well-chosen examples to illustrate the key features of each chosen web-based too which is sufficient for the bottom of mark band 3.l There is possibly too much text on some of the slides which could distract from the primary purpose of the presentation which is to illustrate the functions of the software. It is not really designed to be delivered to an audience it lends itself more to be read on-screen. The slides should be designed to convey key messages only; the details should appear in the speaker notes and/or handouts. This combination should then enable the audience to make an informed decision based upon the presentation. Holistically there is enough technical evidence within the presentation to meet the requirements of mark band 3 but the construction of the actual presentation stops the awarding of high marks in this band.Mark Awarded 12.Strand dMark Band 1 Mark Band 2 Mark Band 3The report:•identifies some of the communication needs of a small business (SME)• makes some recommendations, for internet connectivity, security procedures, an internet access policy and use of email• is written in simple, non-technical language. The report:•describes most of the communication needsof a small business (SME)• makes detailed recommendations, with somejustification, for internet connectivity, securityprocedures, an internet access policy and useof email• is clearly presented in simple, non-technicallanguage.The report:•describes all the communication needs ofa small business (SME), both current andfuture• makes detailed and appropriaterecommendations, with full justification,for internet connectivity, securityprocedures, an internet access policy anduse of email•is effectively presented in simple, non-technical language, demonstrating fullawareness of audience and purpose.(0 - 10)(11 - 15)(16 - 20) Comments: The candidate has produced a very well detailed report in simple non-technical language which investigates many of the communications options available to an SME. Within the report, the candidate discusses the communication needs in specific terms and includes sensible recommendations for the four elements which need to be covered.The candidate has covered all the requirements of Mark Band 2 and has included both current and future needs but has not submitted the additional evidence of fully justifying each recommendation in order to move into Mark Band 3.Mark Awarded 15Overall CommentsThe eportfolio is relatively easy to access. The candidate has used folders and a sensible link (index.htm) to start the eportfolio. The Index page contains the links to supporting evidence but it is sometimes difficult to return to the main index from some of the sections.The candidate is clearly working at AS level for this qualification with much of the evidence being of a high standard.The end mark reflects a candidate working at the A/B grade boundary area and is clearly capable of achieving a higher grade A mark. Note – the boundaries can change each year at Awarding.Overall Mark Awarded 47/60。
lcia简便自动化培训ppt
介绍LCIA的定义、目的、原则和常用方法,以及其在环境影响评估中的应用。
LCIA基本概念、原则和方法
介绍一款常用的LCIA简便自动化工具,包括其功能、特点和使用方法。
LCIA简便自动化工具
讲解如何收集和处理LCIA所需的数据,包括生命周期清单数据和环境影响因子数据。
数据收集和处理
讲解如何进行LCIA分析,包括影响潜力和影响严重性分析,以及如何编写LCIA评估报告。
定制化服务
借助云技术,实现数据共享和远程操控,提高便利性。
云端化趋势
感谢您的观看
THANKS
自动化技术是指通过计算机、电子和机械等技术手段,实现生产过程的自动化控制和操作,提高生产效率、降低成本的技术。
自动化技术涉及多个领域,包括控制理论、计算机科学、电子工程等,是现代工业生产中不可或缺的重要部分。
VS
未来自动化技术将朝着智能化、网络化、集成化方向发展,实现更加高效、精准、智能的生产和服务。
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某企业LCIA简便自动化实施案例,介绍实施背景、解决方案和实施效果。
案例一
某行业LCIA简便自动化应用案例,介绍应用场景、技术方案和效益分析。
案例二
某跨国公司LCIA简便自动化转型案例,介绍转型过程、关键因素和经验教训。
2021IBM_L2考试真题模拟及答案(2)
2021IBM_L2考试真题模拟及答案(2)1、对于生产过程中引入的有机溶剂,下面哪种说法正确?()(多选题)A. 应在后续的生产环节给予有效去除B. 正文已明确列有残留溶剂检查的品种,必须对生产过程中引入的有机溶剂依法进行该项目检查C. 未在残留溶剂项下明确列出的有机溶剂,可以不检查D. 未在正文中列有此项检查的各品种,如生产过程中引入或产品中残留有机溶剂,均应按通则残留溶剂测定法检查并应符合相应溶剂的限度规定试题答案:A,B,D2、某客户每天要进行备份的应用多达25个,每个应用需要备份的数据量都不大。
之前采用物理磁带库进行数据备份,配置了2个LTO3的磁带驱动器。
据用户反映,目前存在备份窗口内无法完成备份任务的问题。
在这种情况下,推荐用户怎样解决当前问题?()(单选题)A. 将LTO3磁带驱动器替换为LTO4B. 再增加2个LTO4磁带驱动器C. 增加磁带槽位D. 推荐用户使用虚拟带库提高并行备份任务数试题答案:D3、工艺规程如需更改,应当按照()修订、审核、批准。
(单选题)A. 注册批件B. 相关的操作规程C. 质量要求D. 部门规定试题答案:B4、TS3100支持的最大槽位数为()?(单选题)A. 1个B. 9个C. 24个D. 48个试题答案:C5、文件应当()、条理分明,便于查阅。
(单选题)A. 分类存放B. 编号管理C. 分类发放D. 逐份存放试题答案:A6、在某些情况下,性能确认可与()结合进行。
(多选题)A. 运行确认B. 安装确认C. 工艺验证D. 设备确认试题答案:A,C7、为了避免筛网、冲具污染到生产物料,下列做法正确的是()。
(多选题)A. 不用筛网、冲具进行生产B. 挑选不易脱落的材质C. 应定期更换D. 有相应的保护措施试题答案:B,C,D8、一个客户有一个IO比较敏感,比较耗用缓存的应用,下面哪方面可能对性能影响比较明显?()(单选题)A. 写缓存B. 服务器中的高速缓存C. 磁盘驱动器机械臂D. 磁盘阵列中的高速缓存试题答案:C9、中药饮片生产企业可从下列哪些途径购入中药材?()(多选题)A. 供货商B. 农户C. 药材市场D. 农贸市场试题答案:A,B,C,D10、DS5300最多支持的主机登录数量是()。
ASTM材料与实验标准.E260
Designation:E260–96(Reapproved2006)Standard Practice forPacked Column Gas Chromatography1This standard is issued under thefixed designation E260;the number immediately following the designation indicates the year of original adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.A superscript epsilon(e)indicates an editorial change since the last revision or reapproval.1.Scope1.1This practice is intended to serve as a general guide to the application of gas chromatography(GC)with packed columns for the separation and analysis of vaporizable or gaseous organic and inorganic mixtures and as a reference for the writing and reporting of GC methods.N OTE1—This practice excludes any form of gas chromatography associated with open tubular(capillary)columns.1.2This standard does not purport to address all the safety concerns,if any,associated with its use.It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.Specific hazard statements are given in Section8and9.1.3.2.Referenced Documents2.1ASTM Standards:2E355Practice for Gas Chromatography Terms and Rela-tionshipsE516Practice for Testing Thermal Conductivity Detectors Used in Gas ChromatographyE594Practice for Testing Flame Ionization Detectors Used in Gas or Supercritical Fluid ChromatographyE697Practice for Use of Electron-Capture Detectors in Gas ChromatographyE840Practice for Using Flame Photometric Detectors in Gas ChromatographyE1140Practice for Testing Nitrogen/Phosphorus Thermi-onic Ionization Detectors for Use In Gas Chromatography 2.2CGA Publications:3CGA P-1Safe Handling of Compressed Gases in Contain-ersCGA G-5.4Standard for Hydrogen Piping Systems at Con-sumer LocationsCGA P-9The Inert Gases:Argon,Nitrogen and Helium CGA V-7Standard Method of Determining Cylinder Valve Outlet Connections for Industrial Gas MixturesCGA P-12Safe Handling of Cryogenic LiquidsHB-3Handbook of Compressed Gases3.Terminology3.1Terms and relations are defined in Practice E355and references therein.4.Summary of Practice4.1A block diagram of the basic apparatus needed for a gas chromatographic system is as shown in Fig.1.An inert, pressure orflow-controlled carrier gasflowing at a measured rate passes to the injection port or gas sample valve.A sample is introduced into the injection port,where it is vaporized,or if gaseous,into a gas sample valve,and then swept into and through the column by the carrier gas.Passage through the column separates the sample into its components.The effluent from the column passes to a detector where the response of sample components is measured as they emerge from the column.The detector electrical output is relative to the concentration of each resolved component and is transmitted to a recorder,or electronic data processing system,or both,to produce a record of the separation,or chromatogram,from which detailed analysis can be obtained.The detector effluent must be vented to a hood if the effluent contains toxic substances.4.2Gas chromatography is essentially a physical separation technique.The separation is obtained when the sample mixture in the vapor phase passes through a column containing a stationary phase possessing special adsorptive properties.The degree of separation depends upon the differences in the distribution of volatile compounds,organic or inorganic,be-tween a gaseous mobile phase and a selected stationary phase that is contained in a tube or GC column.In gas-liquid chromatography(GLC),the stationary phase is a nonvolatile liquid or gum coated as a thinfilm on afinely-divided,inert1This practice is under the jurisdiction of ASTM Committee E13on MolecularSpectroscopy and is the direct responsibility of Subcommittee E13.19on Chroma-tography.Current edition approved March1,2006.Published March2006.Originallyapproved st previous edition approved in2001as E260–96(2001).2For referenced ASTM standards,visit the ASTM website,,orcontact ASTM Customer Service at service@.For Annual Book of ASTMStandards volume information,refer to the standard’s Document Summary page onthe ASTM website.3Available from Compressed Gas Association,Inc.,1725Jefferson DavisHighway,Arlington,Suite1004,V A22202-4102.Copyright©ASTM International,100Barr Harbor Drive,PO Box C700,West Conshohocken,PA19428-2959,United States.support of a relatively large surface area,and the distribution is based on partition.The liquid phase should not react with,and should have different partition coefficients for,the various components in the sample.In gas-solid chromatography (GSC),the stationary phase is a finely divided solid adsorbent (see 4.4).4.2.1After separation in the analytical column,the compo-nents are detected,and the detector signal is related to the concentration of the volatile components.Tentative identifica-tions can be made by comparison with the retention times of known standards under the same conditions,either on a single column or preferably by injecting the sample onto two columns of different selectivity.Ancillary techniques,such as mass spectrometry or infrared spectrophotometry,are generally nec-essary for positive identification of components in samples.4.2.2Prior to performing a GC analysis,the following parameters must be considered:4.2.2.1Sample preparation.4.2.2.2Stationary phase and loading on support.4.2.2.3Column material required.4.2.2.4Solid support and mesh size.4.2.2.5Column length and diameter.4.2.2.6Instrument and detector type that will be needed.4.2.2.7Injector,column oven,and detector temperatures required for analysis.4.2.2.8Injection techniques,such as flash volatilization,on-column technique,purge and trap,pyrolysis,etc.4.2.2.9Carrier gas and flow rate.4.2.2.10Data handling and presentation.4.3In gas-liquid chromatography,the degree of separation possible between any two compounds (solutes),is determined by the ratio of their partition coefficients and the separation efficiency.The partition coefficient,K ,is the ratio of the solute concentration in the liquid phase to the solute concentration in the vapor phase at equilibrium conditions.The partition coef-ficient is affected by temperature and the chemical nature of the solute (sample)and solvent (stationary phase).4.4Another mechanism for separation is gas-solid chroma-tography.With this technique there is no liquid phase,only a porous polymer,molecular sieve,or solid adsorbent.Partition is accomplished by distribution between the gas phase and the solid phase.4.5After the sample is resolved into individual components by the chromatographic column,the concentration or mass flow of each component in the carrier gas can be measured by an appropriate detector which sends an electrical signal to arecording potentiometer or other readout device.The curve obtained by plotting detector response against time is referred to as a chromatogram.For flame ionization and thermal conductivity detectors,either the peak areas or the peak heights are proportional to the concentration of the components in the sample within the linear range of the detector system.How-ever,response fractors are not necessarily the same for all compounds,and linearity of detector response may depend on operating conditions.(Testing of detector performance is discussed in ASTM Standard Practices for the appropriate detector,see 2.1).4.6Components in a mixture may be tentatively identified by retention time.Ideally,each substance has a unique reten-tion time in the chromatogram for a specific set of operating conditions.However,caution is required because the GC separation may be incomplete and a single peak may represent more than one compound.This is especially true of unknown mixtures and complex mixtures because of the very large number of possible compounds in existence and the finite number of peaks that a chromatograph might resolve.Addi-tional characterization data may be provided by ancillary techniques,such as spectrometry.5.Significance and Use5.1This practice describes a procedure for packed-column gas chromatography.It provides general comments,recom-mended techniques,and precautions.A recommended form for reporting GC methods is given in Section 14.6.Apparatus6.1Carrier Gas System —Common carrier gases are helium and nitrogen.Paragraph7.6provides more details on carrier gases.Means must be provided to measure and control the flow rate of the carrier gas.Any flow or pressure control and measurement combination may be used that will give an accurately known and reproducible flow rate over the desired range.6.1.1The main gas supply is regulated with a two-stage regulator which must have a stainless steel diaphragm.Rubber or plastic diaphragms permit oxygen or water to diffuse into the carrier gas.In addition,instruments will have a flow controller between the pressure regulator and column inlet to maintain a constant flow during temperature programming.Copper or stainless steel carrier gas lines,not plastic tubing,should be used to avoid diffusion of oxygen (air)into the carrier gas.When using the thermal conductivity detector,variations intheFIG.1Block Diagram of a Basic Gas ChromatographicSystemflow will change retention and response.The carrier gas line pressure must be higher than that required to maintain the columnflow at the upper temperature limit for theflow controller to operate properly.A pressure of40to60psi is usually sufficient.6.2Column Temperature Control—Precise column tem-perature control is mandatory if reproducible analyses are to be obtained.Temperature control must be within0.1°C if reten-tion times are to be compared with another instrument.6.2.1Air Bath—The thermostated forced-air bath is gener-ally accepted as the best practical method of temperature regulation for most applications.Temperatures can be con-trolled by regulators or proportionally controlled heaters using a thermocouple or platinum-resistance thermometer as a sens-ing element.The advantage of a forced-air bath is the speed of temperature equilibration.Air bath ovens are readily adaptable to temperature programming and are capable of operating over a range of35to450°C.This range can be extended down to−100°C by using cryogenic equipment.6.2.2Other Devices—Liquid baths,drying ovens,incuba-tors,or vapor jacket enclosures are less stable,less convenient means of providing a source of heat to maintain or raise the temperature of a chromatographic column.These devices are not recommended for precision chromatographic applications.6.3The Injection Port—The purpose of the injection port is to introduce the sample into the gas chromatographic column by instantaneous volatilization following injection into the gas chromatographic system.Two sample inlet types are in com-mon use in gas chromatography:theflash vaporization and the on-column injection inlets.6.3.1The temperature of theflash vaporization inlet should be above the boiling points of the sample components and is limited by the amount of septum bleed generated and the temperature stability of sample components.It should be set at that temperature above which no improvement in peak shape occurs but should be determined by the nature of the sample and the volume injected,not by the temperature of the column. If the inlet temperature is too low,broad peak with a slowly rising front edge will result from slow vaporization of the sample.If the temperature is set far above what is necessary to produce fast vaporization,thermal decomposition of the sample,decreased septum life,and ghost peaks due to septum bleed may be observed.Generally,a good guideline is to maintain the inlet temperature25to30°C higher than the highest boiling point of any sample component.6.3.2A glass liner placed inside the injection port will eliminate sample contact with hot metal inner walls of the inlet, which can catalyze thermal decompositions.Any debris left in the liner,especially from biological samples,can be a source of excessive sample adsorption.If a liner is used,the debris can easily be removed by replacing the liner.Deactivation of the glass liner by treatment with dimethyldichlorosilane may be necessary for some compounds.6.3.3With on-column injection technique,the sample is deposited in the liquid state directly on the column packing. The sample must be small enough to precludeflooding of the column,with possible detrimental effects to peak shape and column life.Ideally,the on-column inlet is a part of the column,so its temperature may be controlled as the column temperature is controlled.In practice,because an on-column inlet usually has a somewhat higher thermal mass than an equivalent sector of the rest of the column,the inlet must be heated somewhat above the maximum analysis temperature of the column oven.The criteria of good peak shape and quantitation should be used to determine the maximum re-quired temperature for the inlet.One should consider the temperature limit of the column packing when heating the injection inlet and detector.With some samples,a nonheated injection port is adequate,especially with temperature-programmed operation.6.3.4Injection Port Septum:6.3.4.1The septum is a disc,usually made of silicone rubber,which seals one end of the injection port.It is important to change the septum frequently after two to three dozen injections,or preferably at the end of the working day.The best technique is to change the septum when the column is relatively cool(below50°C)to avoid contact of stationary phase in a hot column with air(danger of oxidation).After the septum is changed,return the inlet temperature to that which was originally set.The inlet temperature should be the opti-mum for the particular analysis,as well as within the recom-mended operating temperature of the septum.If the septum is punctured too many times,it will leak air into the gas chromatographic system,even though it is under pressure.At high temperatures,above150to200°C,air(oxygen)in the carrier gas from a septum leak will degrade the stationary phase.An excessive septum leak will also produce a change in carrier gasflow rate(a change in retention time)and loss of sample(irreproducible peak heights)due to outflow from the leak.When installing the septum,do not overtighten the retaining nut.The septa will swell at high temperature and extrude out of the injection port.A snugfit at room temperature is sufficient.It is important for septum life to make sure the injection needle is sharp with no bent tip.Fine emery cloth,or afine sharpening stone,can be used to sharpen the point. 6.3.4.2Ghost peaks may be observed in temperature pro-grammed runs due to septum bleed.Septum bleed is due to the thermal decomposition,300°C or higher,of the septum that produces primarily lower molecular weight cyclic dimethylsi-loxanes.It contributes to baseline response and is frequently observed as evenly spaced peaks in a temperature programmed run in which no sample has been injected.This situation can be demonstrated by the disappearance of ghost peaks after placing aluminum foil(pre-cleaned with solvents such as methylene chloride or toluene)over the inner face of the septum or by turning off the injector temperature and making several blank runs.Septum bleed can be decreased by using either air-or water-cooled septum retaining nuts,by using a septumflush head,or by using special high-temperature septa which are available from a number of gas chromatographic supply houses.6.4Detector Temperature Control—The detector tempera-ture should always be above that of the maximum operating analytical temperature,to prevent the possibility of condensa-tion of sample components or stationary phase bleed in the detector and connecting line.Because there is usuallysometemperature gradient across a detector,the temperature should be set at 30to 50°C above the maximum analysis temperature to ensure that the entire detector is hot enough to prevent ually,it is neither necessary nor desirable to use an excessively high temperature since this can result in reduced sensitivity,increased noise level,frequent need to clean the detector,and thermal decomposition of sample or stationary phase.6.5Measurement of Temperature —The choice of sensing elements used to measure temperature depends on the desired accuracy (control about a set point)and precision of the measurements.Instrument read-outs should be verified peri-odically.Some common temperature measurement devices are as follows:6.5.1Standardized Mercury Thermometer :Range,°C Accuracy,°C 0to 10060.02100to 20060.05200to 40060.506.5.2Calibrated Platinum Resistance Thermometer:Range,°C Accuracy,°C −140to 50060.016.5.2Thermocouple (iron −constantan,or other).6.6Analytical Column :6.6.1The analytical column is a length of tubing (glass,metal,or plastic)that is filled with a packing material.It is discussed thoroughly in Section7.6.6.1.1Column Characteristics —Specified by method.6.6.1.2Carrier Gas —Specified by method.6.6.1.3Sample Size —Variable within limits.6.6.1.4Flow Rates of Carrier Gas and Detector Gases —Variable within limits.6.6.1.5Column Temperature —Usually specified by method,and6.6.1.6Physical or Chemical Characteristic of Compound Analyzed ,or both.6.6.2Detector Characteristics —Desirable detector charac-teristics should include the following:6.6.2.1Good stability (low noise level,minimum response to changes in temperature and flow rate).6.6.2.2Ruggedness and simplicity.6.6.2.3Sensitivity to the components for which analysis is e either a selective detector for materials of interest or one with a similar response for all components.6.6.2.4Linearity of response versus sample concentration.Wide linear range.6.6.2.5Rapid response to changes in column effluent com-position (small internal volume or flow-through design,or both).6.6.2.6Detectors,which are nondestructive and do not contribute to band broadening may be used in series with other detectors.6.7Types of Detectors —The detector is located at the outlet end of the chromatographic column and both senses and measures the amount of components that have emerged from the column.The optimum detector should have high sensitiv-ity,low noise level,a wide linearity of response,a response to all compounds of interest,and yet be insensitive to changes in flow and temperature.Selective detectors are characterized as having selective,or greatly enhanced response to certain components.Linearity is decreased for all detectors by column bleed.As many as forty detection systems have been reported,yet only about a dozen are commonly used.Table 1shows some of the more commonly used detectors.Of these,the thermal conductivity,the flame ionization,the electron capture,the nitrogen-phosphorus,and the flame photometric detectors are the most popular.Nondestructive detectors should be vented to a hood to remove any toxic effluents from the workplace.The effluent from destructive detectors may also be toxic.Details on detectors can be found in the applicable methods in Practices E 516,E 594,E 697,E 840,and E 1140.6.8Programmed Temperature Operation —The apparatus used in programmed temperature gas chromatography differs in some respects from that normally used for isothermal work.Basically,the column temperature is varied with time (program rate)to enhance speed of separations.The advantages of using programmed temperature operation include better resolution of lower boiling components because of lower starting tempera-ture and greater sensitivity because of sharper peaks for the higher boiling components.6.8.1Column Heater and Temperature Programmer —It is of utmost importance that the column temperature program be reproducible,and that the difference between the set (desired)temperature and the true average column temperature be as small as possible.However,these requirements are difficult to achieve at high heating rates and with columns of large diameter.The mass of the column and its heater should be kept as small as possible.This will minimize thermal lag and will give proportionately small variations around the set tempera-ture at any time.Proportional temperature controllers supply almost full power to the heater until the set point is very closely approached.6.8.2The recirculating air bath is the recommended method of heating in programmed temperature gas chromatography (PTGC).The obvious advantages are extremely rapid heating (and cooling after an analysis is completed)with very little temperature lag.TABLE 1Applicability of Commonly Used Gas Chromatographic DetectorsDetector AApplicability(Type of Compound)Range of Minimal DetectableAmounts (grams)Thermal Conductivity All10−6to 10−7Flame Ionization Organic10−12Electron Capture Halogenated and Oxygenated 10−12to 10−15Flame Photometric Sulfur,Phosphorus 10−11Alkali FlameNitrogen,Phosphorus10−12(even lower for phosphorus)AFurther information can be found in Practices E 516,E 594and E 697.6.8.3Liquid baths may be used for very low heating rates.They are commonly contained in taped Dewar flasks.6.8.4No matter what type of heating device is used,accurate control of the temperature program is necessary.This is usually accomplished by appropriate electronic systems that develop linear (or other)programming rates as desired.6.8.5Detectors for programmed temperature gas chroma-tography should be relatively insensitive to minor temperature and flow fluctuations and insensitive to stationary-phase bleed.These difficulties can be overcome by operating the detector at or near the upper temperature limit for the analysis and by using adequate flow controllers.If stationary-phase bleeding is excessive during PTGC runs,a second conditioning procedure (Section 9)might improve the situation.Alternately,a dupli-cate analysis column can be used on the reference side of the detector.By equalizing substrate bleed on both sides of the detector,the baseline drift can be substantially compensated.However,this technique does not improve column life and is detrimental to detector linearity.If at all possible,operate the column within its recommended temperature range.6.8.6When using the temperature programming technique,the resistance to carrier gas flow in the gas chromatographic column increases with increasing temperature.The flow con-trollers need a positive pressure of 10psi to operate properly.By setting the second stage of the regulator to 40to 60psi,there will usually be sufficient excess pressure to maintain a constant gas flow through the column.Higher pressures might be required to maintain flow when using relatively long columns of 10ft,or longer,or packings finer than 120mesh.7.Materials7.1Stationary Phases —The stationary phases (partitioning agents)that have been successfully used for specific separa-tions are found most quickly by a literature search.Many phases are listed in ASTM publications AMD-25A and AMD-25A-51.4The most desirable stationary phases do not volatilize (bleed)significantly from the solid supports at temperatures required to elute the sample.7.1.1The polarity of the stationary phases is currently best characterized by McReynolds Constants.5The higher the McReynolds Constant,the more polar the phase.Rohr-schneider constants can also be used to measure the polarity of stationary phases.67.1.2The effects of using polar and nonpolar stationary phases are summarized as follows:7.1.2.1Nonpolar stationary phases separate compounds pri-marily by order of relative volatility or boiling point.7.1.2.2Polar stationary phases separate compounds by or-der of both relative volatility and relative polarity.With polar phases,nonpolar compounds will elute before polar com-pounds of the same boiling point.7.1.2.3Polarity alone is insufficient to describe the separa-tion power of a column.One must consider the overall selectivity of a column towards a set of analytes.This selectivity is a summation of the effects of dispersive interac-tions,acid-base interactions and the dipole interactions offered by the various pendent groups in the stationary phase.7.1.3The stationary phases used in gas chromatographic columns have both minimum and maximum temperature limits.The chromatographer must be aware of the limits for the phase being used.Below the minimum temperature,the phase will behave as either a viscous liquid or a solid.Less efficient separation will be observed,and the chromatographic results will be exhibited as broader peaks in the gas chromatogram due to poor mass transfer of components in the stationary phase.7.1.3.1Above the maximum temperature limit,the phase will begin to bleed off the column at an accelerated rate,and the observed results will include a drifting baseline or exces-sive spiking on the baseline.Under these conditions,the liquid phase will decompose or volatilize,and thus be removed from the column.This situation will eventually lead to decreased retention times with broader peaks resulting in poorer resolu-tion of very close peaks.Peak tailing will also be observed as the uncoated surface becomes exposed by removal of liquid phase,thus shortening column life.Bleeding also can expose bare support surface that can adsorb molecules being analyzed and reduce column efficiency.In extreme cases,phase bleeding will result in fouling the detector and connecting lines.The observed maximum temperature will depend upon many ex-perimental variables,such as type of liquid phase column,conditioning,phase-loading level,column temperature,sensi-tivity setting of the detector,and purity of the carrier gas.In programmed temperature runs,the column can sometimes be operated for short periods about 25°C above maximum tem-perature.However,column bleed should be minimized for quantitative results since it decreases the linear range of all detectors.7.2Active Solids :7.2.1Molecular Sieves —The synthetic zeolite molecular sieve sorbents separate molecules by size and structural shape.Isomers with a more round shape,as branched versus straight chain molecules,diffuse in and out of the zeolite structure more easily than isomers with the long chain structures.Separations are affected by the differences in times required for molecules of different sizes to find their way into and out of the sieve-like structure of the adsorbent.Molecular sieves are most useful for separating H 2,O 2,N 2,CO,and CH 4.Carbon molecular sieves are also available,and can be used to separate O 2,N 2,CO,CO 2,H 2O,and C 1to C 4hydrocarbons.7.2.2Porous Polymers :7.2.2.1One type of porous polymer used in gas chromatog-raphy is available in the form of microporous cross-linked,polymer beads produced by copolymerizing styrene and divi-nylbenzene or more polar copolymers,or both.These materials are generally used as received without coating with any liquid phase.They provide symmetrical peaks for polar,hydrogen-bonding compounds such as water,alcohols,free acids,4Gas Chromatographic Data Compilation,ASTM,1981.5McReynolds,W.O.,Journal of Chromatography Science ,V ol 8,1970,p.685.6Supina,W.R.,and Rose,L.P.,Journal of Chromatography ,V ol 8,1970,p.214.amines,ammonia,hydrogen sulfide,etc.,and organic com-pounds up to molecular weights corresponding to about170.7.2.2.2Another porous polymer is poly(2,6-diphenyl-p-phenylene oxide).This material is useful for the analysis of amines,alcohols,and hydrogen-bonding compounds.It is also used as an adsorbent for trapping trace organic compounds in water and air.7.2.3Silica Gel,Alumina,and Carbon—Among the active solid adsorbents are silica gel,alumina,and activated carbon. They are useful for low-boiling hydrocarbons.7.2.4Solid adsorbents modified by low concentrations of liquid phases may retain the advantageous properties of both. Some solid adsorbents can be modified by the addition of surface activating compounds such as wetting agents,silver nitrate,and the metal salts of fatty acids.7.3Diatomaceous Earth Supports—The most popular gas chromatographic supports are those prepared from diatoma-ceous earth,also called diatomaceous silica or kieselguhr.The two main types are white and pink in color.The white supports are recommended over the pink supports because of their more inert surface.The former are,however,very friable and must be handled very carefully when preparing packings and loading into gas chromatographic columns.Before using these sup-ports,check the manufacturer’s literature for comments on their use.7.3.1The white-colored supports are produced by calcina-tion of diatomaceous earth with sodium carbonate as aflux.In this process,the diatomaceous earth fuses,due to formation of a sodium silicate glass.The product is white in color due to conversion of iron oxide into a colorless complex of sodium iron silicate.These white materials are used to prepare the more inert gas chromatographic supports.However,they are fragile and subject to abrasion from excessive handling in the course of sieving,packing,or shipping.Abrasion will produce finer particles,orfines,which will decrease column efficiency.7.3.2The pink-colored supports are prepared by crushing diatomaceous earthfirebrick that has been calcined with a clay binder.The metal impurities remaining form complex oxides that contribute to the pink color of the support.These pink supports are denser than the white supports because of the greater destruction of the diatomite structure during calcina-tion.They are harder and less friable than the white supports and are capable of holding larger amounts of liquid phase(up to30%)without becoming too sticky toflow freely.Their surface is generally more adsorptive than white supports.For this reason,they are not recommended for use in the gas chromatographic analysis of polar compounds.However,pink supports provide excellent efficiencies for the analysis of hydrocarbons and organic compounds of low polarity.7.3.3Chemical Treatment of Diatomaceous Earth Supports—Neither the pink nor the white materials give generally acceptable analysis of more polar compounds with-out further treatment.With these compounds,severe peak tailing is often observed,especially with the dense pink supports.This tailing is due to the presence of adsorptive and catalytic centers on all diatomaceous earth supports.The adsorptive sites are attributed to metal oxides(Fe,Al)and surface silanol groups,-SiOH,on the support surface.The latter are capable of forming hydrogen bonds with polar compounds.7.3.3.1Metal impurities are removed by washing with hydrochloric acid,which leaches out iron and aluminum and renders the surface both less adsorptive and less catalytically active.However,even with acid washing,the pink supports are still more adsorptive toward polar compounds than the white-type supports.Acid washing is sometimes followed by base washing,which seems to remove only minor amounts of metal impurities,but is a good pretreatment for supports that are to be used for the analysis of basic compounds.7.3.3.2Neither acid or base washing is effective in reducing peak tailing due to hydrogen bonding with the surface silanol groups,-SiOH.These groups are most effectively masked by treatment with dimethyldichlorosilane.7.3.4Acid-washed silanized grades of white diatomaceous earths are recommended as supports for nonpolar and medium polarity liquid phases.Because of the hydrophobic character of a silanized diatomaceous earth,even coating of the most polar liquid phases is difficult to achieve.Acid-washed,silanized grades of white diatomaceous earths are recommended as supports for the polar liquid phases,such as polyesters and silicones of high cyano-group content.7.3.5If the column is6ft(2m),or less,use particle size of 100to120mesh(125to149µm)for highest efficiency under isothermal conditions.If the column is longer than6ft,use80 to100mesh(149to177µm)particles.If temperature pro-gramming is used,80to100mesh particles should be used to lessen resistance to carrier gasflow.7.3.6Further information concerning the liquid phase load-ing is given in9.3.7.4Halocarbon Supports—The two types of halocarbon supports are those prepared from poly(tetrafluoroethylene)and poly(chlorotrifluoroethylene).These supports are relatively inert and are nonpolar.They eliminate peak tailing observed in the analysis of organic compounds capable of hydrogen bond-ing,such as water,alcohols,amines,etc.They are the preferred supports in the analysis of corrosive halogen compounds such as HF,BCl3,UF6,COCl2,F2,and HCl.7.4.1Poly(tetrafluoroethylene)supports require special han-dling procedures.When used as received,they are soft and tend to form gums upon handling.They can also build up a static charge and spray out of the column during the packing operation.These problems can be virtually eliminated by cooling the support to0°C before coating with liquid phase and by avoiding the use of glass vessels.Rinsing poly(tetrafluoro-ethylene)with methanol and drying before use is another way to eliminate the static-charge problem.7.4.2Supports prepared from poly(chlorotrifluoroethylene) are structurally harder and are much easier to handle and to pack into a column.7.5Tubing Materials—Tubing materials should be chosen on the basis of the following criteria:7.5.1They should be nonreactive with the stationary phase, sample solvent,and carrier gas.7.5.2They should possess physical properties to withstand temperature and pressure of operating conditions,and。
ells实验流程设计
ells实验流程设计下载温馨提示:该文档是我店铺精心编制而成,希望大家下载以后,能够帮助大家解决实际的问题。
文档下载后可定制随意修改,请根据实际需要进行相应的调整和使用,谢谢!并且,本店铺为大家提供各种各样类型的实用资料,如教育随笔、日记赏析、句子摘抄、古诗大全、经典美文、话题作文、工作总结、词语解析、文案摘录、其他资料等等,如想了解不同资料格式和写法,敬请关注!Download tips: This document is carefully compiled by theeditor. I hope that after you download them,they can help yousolve practical problems. The document can be customized andmodified after downloading,please adjust and use it according toactual needs, thank you!In addition, our shop provides you with various types ofpractical materials,such as educational essays, diaryappreciation,sentence excerpts,ancient poems,classic articles,topic composition,work summary,word parsing,copy excerpts,other materials and so on,want to know different data formats andwriting methods,please pay attention!1. 实验准备确定实验目的和检测指标。
选择合适的 ELISA 试剂盒,根据试剂盒说明书准备所需的试剂和材料。
P Lc实训报告
P Lc实训报告时间过得真快,转眼就到了我们实习的时间,这次实习使我对 pc程序有了更深刻的认识,为以后的工作打下坚实的基础。
虽然这是我大学生涯中第一次正式接手一项系统的编程工作,但对自己能够独立完成这样一个程序还是有相当大的信心了。
在做完开发任务后我就开始投入到实际操作当中了,这次实习我主要是通过观察代码来学习 pc编程与程序设计之间的关系及流程与技巧。
首先,我们先来看一下编程语言:pc语言是一种非常高级的软件开发语言,它以使用 C#作为基本语言,并采用 Java作为主要编程工具和实现手段为特征。
主要用于设计程序框架和接口。
在完成一个项目时必须首先编写程序和开发相应的功能应用场景,然后根据该应用场景编写相应的代码、实现相应功能。
在实际项目中,针对不同应用场景、不同用户需要而设计开发一些功能强但是使用复杂度较高的程序都可以通过应用程序模块实现相关自动化功能。
所以我们主要以 Pc环境开发为例来介绍 pc语言。
一、 pc环境开发步骤a.首先从硬件环境开始,运行 pc环境运行测试环境中的所有程序。
b.用 pc的图形界面来展示整个 pc环境,并对其进行配置。
c.开始将 Pcserver文件插入到 pc应用程序模块,创建pcserver的配置文件(图1)为环境配置,用 pc界面来显示环境的所有功能,并在环境创建好后对环境进行配置。
d.当应用程序配置完毕后,返回 pcserver文件中对环境进行配置并显示其主要功能及效果,这时再进行修改。
e.根据实际应用场景对实现结果进行调试(图2)。
f.最终结果并返回 pcserver文件完成环境应用程序的开发与调试工作(图3)。
f.最后对这个环境进行维护,实现环境相关功能和效果。
f.最后将该环境发布到 pc工具中去。
整个过程其实就是一个页面构建的一次循环,而开发完这个页面之后再利用 java构建出一个完整的 pc文件就完成了完整的开发过程,而最终呈现给大家的就是我们理想中呈现在眼前一款软件程序产品罢了。
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INTERNATIONAL ENGLISHLANGUAGE ASSESSMENT(IELA)IELA PRACTICE TESTINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)VOCABULARY - PART ONEInstructions: From the choices given (A, B, C or D) find the word which is nearest in meaning to the word on the left.Word A B C D1) Compile Mix Compel Assemble Disassemble2) Restore Renovate Maintain Back Status quo3) Trigger Motivate Inculcate Instigate Instill4) Likewise Otherwise Similarly Moreover Example5) Predominant Distinct Important Dormant Pre-eminent Answers: The correct answers are as follows:1) C2) A3) C4) B5) BINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)VOCABULARY - PART TWOInstructions: Fill in the gaps with the correct word from “a”, “b”, “c” or “d”6. Women who apply for jobs in middle or senior ________________ have ahigher success rate than mena. managerb. managec. managementd. managerial7. Larger firms, ______________________, appear to be more wiling toexperiment with flexible working arrangements.a. in particularb. particularlyc. particulard. in specific8. Travel is a _____________________ of status in modern societies and isalso thought to be necessary for good health.a. markingb. markerc. mark-outd. marked9. _______________________ at the failure of many of the poor imitations ofModernist architecture led to interest in various styles and ideas from thepast and present.Disillusionmenta. Displeasureb. Dispassionatec. Disillusiond.10. In business as a whole, there are a number of factors encouraging the__________________ of greater equality in the workforce.a. prospectorb. prospectc. prospectusd. prospectiveAnswers:6) c7) a8) b9) d10) bINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)SENTENCE COMPREHENSION Instruction: After each sentence, four possible meanings are given. Choose the sentence which is nearest in meaning to the original sentence.Now answer the following questions:11. Inflation hits the poor hardest.a) Inflation is not good for the poor.b) The poor are hardly affected by inflation.c) The poor are the most affected by inflation.d) The poor are affected by inflation.12. Sports surely mean mega bucks these days.a) These days sports is very popular.b) Investing in sports gives good monetary returns.c) Sports make everyone rich.d) These days’ sports generate less money.13. People who are in the public eye have to become accustomed to a great deal of attention.a) People who are well-known must become used to attention.b) Well-known people should get a lot of attention.c) Well- known people seek attention.d) People who are well-known may become used to attention.14. Money alone can make you happy is still very premature.a) Money alone can make you happy.b) Still you cannot say that only money can make you happy.c) Only money cannot make you happy.d) You are immature if you say money alone can make you happy.15. The visa scenario today is going to impact the aviation industry adversely.a) The visa scenario has affected the aviation industry harmfully.b) The visa scenario today is going to be beneficial for the aviation industry.c) Because of today’s visa scenario aviation industry is going to suffer a great deal.d) The visa scenario today is going to improve the aviation industry soon.Answers: The correct answers are as follows - 11) cb12)13) a14) b15) cINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)READING COMPREHENSIONPassage No. 1Read the following passage and answer the questions that follow:The recent news that local hospitals have had to reroute seriously ill patients because the community’s critical –care beds are full is worrisome. Earlier this week, four of the six local hospitals ran out of space for the critically ill and had to turn people away.Federal law requires hospitals to treat anyone who walks in. As a result of having to treat large numbers of uninsured patients, the emergency rooms often become an economic drain on their hospitals. Doctors now want to set up their own free-standing ambulatory surgical facilities and diagnostic centers. Critics contend this would leave hospitals with less revenues and the same number of indigents to treat.A bill was recently introduced to phase out the need for a “certificate of public need” for non-hospital-based facilities, provided those facilities met stringent regulations and requirements. This would have made it easier to set up alternative facilities to help indigent patients. The finance committee balked at the hefty price and killed the bill, another casualty of a failed legislative session.Unfortunately, the problem of access to medical care for those of limited means is not going to go away anytime soon and, despite the well-intended regulations, too-full hospitals compromise everyone’s welfare. Healthy competition with small neighborhood surgical and diagnostic centers may be what is necessary to help dampen rising medical costs. But under no circumstances should the hospitals be forced to care for everybody without health insurance while competitors operate free of the burden of caring for those unable to pay.Q. 16 The purpose of this passage is primarily to explain thata) Doctors are establishing their own surgical facilities and diagnostic Centres.b) Describe the sad situation prevailing at local hospitals.c) Discuss the need for non-hospital-based facilities.d) Explain the need for health insurance.Q. 17 Look at the phrase “ran out” in line 3. The meaning of this phrasal verbin the passage isa) Short ofb) Letofc) End ofd) CompleteQ. 18 According to the passage, which of the following statements about federal law is true?a) It states that the patient should be insured.b) It states that hospitals should get more revenue.c) Every patient who walks in should be treated.d) Only insured patients should be treated.Q. 19 Look at the word “stringent” in line number 12. Choose the word or phrase that is closest in meaning to the word “stringent”a) Strictb) Stingyclearc) Verycleard) NotQ. 20 Look at the word “this” in line number 13. Choose the word or phrase that the word refers tofacilitiesa) Theb) Regulationsc) Requirementsd) BillThe correct answers are as followsQ 16) bQ 17) aQ 18) cQ 19) aQ 20) dINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)READING COMPREHENSIONPassage No. 2Q.1 Read the following passage and answer the questions.Charging Joe Customer $ 15 for a $ 10 widget argue against honesty as the best policy in the short run. In the short run, ethics and profit are often related inversely, with honesty squeezing the bottom line. If you plan on going out of business after making your first sale, your bottom line will suffer from a policy of honesty. However, if you plan to remain in business, your object must be to make customers, not just sales.Once made, a sale ends. A customer, however, represents many more potential sales. Become known for high ethical standards, and you’ll probably make many customers.In the short run, your honesty may reduce profit: “Joe, I could sell you this $ 20 widget, but for your purpose, our $ 10 model will do just fine.” You’re out $ 10, but Joe will tell Jill that Acme just saved him $10, and when she needs a widget, she’ll come to you. In the long run, ethical firms are more profitable than unethical ones.Your honesty reflects not just on you, but on your entire organization. If your job is selling Acme Widgets, your customers will see you as an honest salesperson and Acme as an honest firm.Equally important, honesty percolates. Your ethical behavior permeates the organization. Act ethically, and your subordinates will probably do the same, as will your peers.Honest organizations build trust not only among customers, but also among the members of the organization. In a climate of trust, people are generally more productive because they aren’t constrained by hovering micromanagers. Productive companies are typically profitable companies.While example is generally the best teacher, don’t just rely on others to pick up on your demonstrations of ethical business behavior. Take time to discuss the role of ethics in your organization. Be clear and explicit: The policy of your business is ethical business.Q.21) The writer’s overall purpose is to tell the reader thata) Your honesty may reduce profit.b) A customer represents many more potential sales.are profitable companies.companiesc) Productived) The policy of your business should be ethical business.Q.22) Why ethics and profit often related inversely?a) Because ethics generates profit.b) Because ethics may decrease the profits.c) Because they are related to each other.d) Because ethics may increase the profits.Q.23) The word ‘squeezing’ in line no. 3 is closest in meaning toa) firmb) putc) pressd) placedQ.24) According to the passage, which of the following statements about ethical business is true?a) In business, your object must be to make more sales.b) Honesty takes a back seat in business.c) The more sales, the more successful your business.d) In business, your aim should be to make more customers, not just sales.Q.25) Look at the phrase ‘honesty percolates” in line no.18.The meaning of this phrase isa) Honesty passes through.b) Honesty is everywhere.c) Honesty is the best policy.d) Honesty is hardly seen is business.AnswersQ. 21) dQ. 22) bQ. 23) cQ. 24) dQ. 25) aINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)READING COMPREHENSIONPassage No. 3Read the following passage and answer the questions:-Who is the busiest retailer in Britain? Marks & Spencer? Sainsbury perhaps or Tesco? Wrong on all three counts. The answer is Richer Sounds, a little-known, privately owned, cut-price retailer of hi-fi equipment with 12 shops in the UK.In this year’s Guinness Book of Records, Richer Sounds warrants an entry for the highest sales per square foot of any retailer in the UK – 16,635 – for its store on London Bridge Walk in the City. Even taking an average across all 12 stores, Richer Sounds clocks up 2500 square feet, five times more than Marks & Spencer, treble the sales achieved by Sainsbury.It is profitable too. Last year- the year of the dog for UK retailing – Richer Sounds made profits of more than £ 5,00,000 on sales of almost £ 12 million.The man behind Richer Sounds’ success is founder, managing director and 98 percent shareholder Julian Richer, a 31-year-old Londoner who, at the age of 14, used to buy and sell candles during the energy crisis of 1974. A likeable bloke who wears his blond hair in a pony tail and operates from a small converted Victorian vinegar factory in Bermondsey, South-East London, he raps out his sales pitch. “Even when I was at school I wanted to go into business”, he says, “but my age was against me. Property was out- I wasn’t old enough to sign a contract. Cars were out- I couldn’t drive. So I turned to hi-fi, which was catching on at school. I bought a second-hand Bang & Olufsen for £ 10, did it up and sold it for £ 22.” In 1979, at the age of 19, he opened his first shop- the one on London Bridge Walk.Since then, little has changed. In simple terms, Richer Sounds sells discounted hi-fi from tiny, basic shops with low overheads. Stock turnover is rapid and the company’s smallness gives it flexibility to take advantage of deals offered by manufacturers on end of line or surplus equipment.The technique has enabled Richer Sounds to secure itself a lucrative niche in a £ 4 billion audio visual market dominated by independents.Marketing is a key weapon. Richer Sounds advertises regularly in national newspapers (“We buy late space at a discount,” Richer says) and in alternativemagazines such as Private Eye and Viz. Every month it produces 1, 00,000 copies of a brochure pushing the latest offers.Good service is another priority. At Richer Sounds staff are trained not to be pushy. They all attend two training seminars a year at Richer’s country house in Yorkshire, where more attention is paid to following the correct administrative procedures.Q. 26 Richer Sounds isa) a department storeb) a big storec) privately owned retailerd) a single outletQ.27 Richer Sounds is cheaper than other hi-fi retailers becausea) It only sells old models of hi-fi equipment.b) It buys equipment from manufactures at special prices.c) It sells second-hand equipment.d) It sells only low quality hi-fi equipment.Q. 28 Richer Soundsa) Does all its advertising through brochures.b) Relies on getting free publicity from newspaper and magazine article.c) Buys advertising space at a low price.d) Doesn’t believe in advertising.Q. 29 According to the passage, which of the following statements about Richer Sounds is true?a) Their staff gets two months training.b) They are the busiest retailers in the entire Europe.c) Their staff attends two training sessions a year.d) Their staff are trained to be pushy.Q. 30 Retailing isa) Selling grocery to the public.b) Selling cloths to the public.c) Covering all forms of selling goods to the public.d) Selling jewellery and cosmetics to the public.Answers: The correct answers are as followsQ.26 cQ.27 bQ.28 cQ.29 cQ.30 cINTERNATIONAL ENGILSH LANGUAGE ASSESSMENT (IELA)READING COMPREHENSIONPassage no 4Read the following passage and answer the questions following:According to statistics it is becoming increasingly rare in many Western countries for families to eat together. It seems that people no longer have time to enjoy a meal, let alone buy and prepare the ingredients. Meanwhile, fast food outlets are proliferating. Further evidence of the effects of the increasing pace of life can be seen on all sides. Motorists drum their fingers impatiently at stop lights. Tempers flare in supermarket queues. Saddest of all is the success of an American series of books called “One Minute Bedtime Stories”. What, one has to ask, do parents do with the time thus saved?According to Barton Sparagon, M.D. medical director of the Meyer Friedman Institute in San Francisco and an expert on strees-related illness, the above are all symptoms of a modern epidemic called “hurry sickness”. The term was coined nearly 40 years ago by a prominent cardiologist, who noticed that all of his heart disease patients had common behavioral characteristics, the most obvious being that they were in a chronic rush. Hurry sickeness has been an issue in our culture ever since, but the problem is escalating in degree and intensity, leading to rudeness, short-tempered behavior and even violence, alongside a range of physical ills.The primary culprit, according to Sparagon, is the increasing prevalence of technology-like e-mail, cell phones, pagers and laptop computers. We can bring work home, into our bedrooms and on our vacations. Time has sped up for so many people, and there is increased pressure to do more in the same number of hours, says Sparagon.Jill Stein, a sociologist at the University of California at Los Angeles, agrees that time is being more compressed than ever. “In the past, an overnight letter used to be a big deal. Now if you can’t send an e-mail attachment, there’s something wrong. Because the technology is available to us, there is an irresistible urge to use it.” What about those annoying people who shout into their cell phones, oblivious to those around them? Stein says that self-centered behavior is related to larger social trends as well as technology.“There is a breakdown of the nuclear family, of community, of belonging; and an increased alienation and sense that we’re all disconnected from one another. This breakdown came before the technology, but the technology has exacerbated it.” Now we connect through this technology, says Stein, and we don’t have face-to-face interaction. Ironically, as people pull their cell phones out in the most unlikelyvenues, our personal lives are available on a public level as never before. People are having work meetings and conversations about their spouses and their therapy sessions with complete impunity. Ordinarily we’d never be exposed to this information, says Stein.“People are finding that all of this multi-tasking, rushing and worrying is not only marking life intolerable, but actually making them less efficient than they could otherwise be.”Q. 31. The purpose of this passage is primarily toa) Explain the consequences of hurry sickness.b) define hurry sicknessc) discuss about Western countriesd) tell about the positive effect of hurry sicknessQ.32. What is the meaning of the line, “Tempers flare in supermarket queues”?a) people get eager to pay the billsb) people get angry standing in the supermarket queuesc) people don’t like to stand in supermarket queuesd) people like to stand in supermarket queuesQ.33. One result of technology and the increased pace of life is that peoplea) frequently meet work colleagues in public placesb) have personal telephone conversations in publicc) need to visit therapists on a regular basisd) no longer have offices to work fromQ. 34 The article above mentions a number of factors that contribute to “hurry sickness’ which one of them is not mentioned?a) using cell phonesb) bringing work homec) communications have become fasterd) globalisation has led to 24-hour trading.Q.35 Look at the word “prominent” in line on 1 2. Choose the word or phrase that is most nearly opposite in meaning to the word “prominent”a) importantb) unimportantc) famousd) notoriousAnswers: The correct answers are as follows:Q. 31) aQ. 32) bQ. 33) bQ. 34) aQ. 35) bInternational English Language Assessment (IELA)WritingQ.36. Read the following paragraph and then from the four sentences given below, choose the best sentence with which the paragraph would start.A survey carried out in 1988 by Britain’s Equal Opportunities Commission (EOC) revealed that 78 per cent of sharers were female, the majority of whom were between the ages of 20 and 40 years of age. Subsequent studies have come up with similar result. Many of these women were re-entering the job market having had children, but they chose not to seek part-time work because it would have meant reduced wages and lower status. Job sharing also offered and acceptable transition back into full-time work after a long absence.a) The types of jobs that are shared may varyb) As would be expected, woman comprise the bulk of job sharers.c) Women love the concept of jobs sharing.d) Job sharing is very good – for women.Q.37. A source may be an individual (speaking, writing, drawing, gesturing) or a communication organization (like a newspaper, publishing house, television station or motion picture studio). The message may be in the form of link on paper, sound waves in the air, impulses in an electric current, a wave of the hand, a flag in the air, or any other signal capable of being interpreted meaningfully. The destination may be an individual listening, watching or reading; or a member of a group, such as a discussion group, a lecture audience, a football crowd, or a mob; or an individual member of the particular group we call the mass audience, such as the reader of a newspaper or a viewer of television.a) Mass communication has three elementsb) Essence of communication is getting the receiver and the sender tunedtogether for a particular massage.c) Communication requires of least three elements the source, the massage,and the destination.d) The source, the massage, and the destination are related to communication. Q.38. Here are four sentences. Arrange them in the correct order so that they form a coherent paragraph. Each sentence is marked 1,2,3,4. Choose the correct order from the four options given below:a) It leaves important but visually uninteresting stories such as governmentbudget and legislation stories with little or no coverage.b) Television news, argue the critics, concentrates mostly on stories of visualinterest.c) Thus, television news, according to this view, presents an image of the worldthat is quite subjective.d) This leads to the claim that the selection of stories to be presented ontelevision news tends less toward information and more toward entertainment.The correct order is (a) a d c b(b) c b a d(c) b c a d(d) b a d cInternational English Language Assessment (IELA)In the following sentences, one word or a phrase is used wrongly. Choose the word that must be changed to make the sentence correct.Q. 39 Provided they are with an adult, children are not allowed to use theswimming pool.a) allowedb) usec) providedd) adultQ. 40 There weren’t too much people at this meeting as the last one.a) asb) weren’tc) atd) too muchQ. 41 Communication is coming from the Latin word “communis”, common.cominga) isLatinb) thec) The sentence is right.d) fromQ. 42 Right guidance is going to give your arrow a perfect direction so that it hits the target.a) is going toperfectb) ac) hitsd) soQ. 43 Beside World War II, Japan became the world’s second largest economy and perfected inclusive growth and equitable wealth model by capitalism.a) equitableb) besidec) perfectedd) becameQ. 44 In recent months there has been a series of mergers of various telecomcompanies.a) mergersb) therec) has beend) of variousQ. 45 Look out of the window. The sky is blue and the sun is shining. It’s abeautiful day, is it?a) out ofb) it’sshiningc) isd) is itQ. 46 At last the Committee were ready to announce their decision. They made up their minds.a) werelastb) atc) maded) toQ. 47 If you had planned things properly, you wouldn’t got into a mess.a) wouldn’tplannedb) hadc) gotd) properlyQ. 48 The island is very peaceful. Life is good here. Everybody moves at nice slow pace.a) The islandb) Lifec) At niced) PaceQ. 49 There was a bank robbery yesterday. The police has arrested five peoplea) hasb) wasc) arrestedd) a bankQ. 50 To be successful in life, you must believe in yourself.a) in lifeb) mustc) yourselfd) the sentence is correctAnswers Q. 36) b Q. 37) c Q. 38) d Q. 39) c Q. 40) d Q. 41) a Q. 42) a Q. 43) b Q. 44) c Q. 45) d Q. 46) c Q. 47) a Q. 48) c Q. 49) a Q. 50) d。