还原型谷胱甘肽对肾急性缺血再灌注性损伤的作用

还原型谷胱甘肽对肾急性缺血再灌注性损伤的作用
蔺艳;何涛;毛晓燕;甘仲霖;张莉;侯静
【期刊名称】《实用医学杂志》
【年(卷),期】2016(032)008
【摘要】目的：探讨还原型谷胱甘肽（GSH）预处理及后处理对大鼠肾脏急性缺血再灌注性损伤（IRI）的作用。

方法：50只SD成年大鼠分成5组：假手术对照组（Sham 组），缺血再灌注组（I／R组），GSH 预处理组（Pre组），GSH后处理组（Post组），每组10只。

Pre组在术前24、16、8 h和45 min时腹腔注射4％ GSH 100 mg／kg。

Post在术后45 min、6、12和18 h腹腔注射同等剂量的GSH。

各组动物制备缺血再灌注模型后第24小时检测血清肌酐（Cr），尿素氮（BUN）水平，总超氧化物歧化酶（T-SOD）活性，丙二醛（MDA）和一氧化氮（NO）含量。

HE染色观察组织病理学改变。

结果：与I／R组相比，Pre组肾组织结构损害明显减轻，Post组无明显病理改变，Pre 组和 Post 组血清 Cr、BUN、MDA 和 NO 含量减少（P ＜0．05），T-SOD 含量增加（P ＜0．05），Post 组T-SOD 含量高于Pre 组（P ＜0．05）。

结论：肾 IR 前或后24 h 内使用 GSH 对肾脏均有保护作用，IR后使用GSH对肾脏的保护作用更
优。

%Objective To investigate the pretreatment and post-treatment effect of reduced glutathione (GSH) on acute ischemia-reperfusion injury (IRI) of rat kidney. Methods Fifty adult SD rats were divided into 5 groups: control group (Sham group), ischemia reperfusion group (I/R group), GSH pretreatment group (pre-treatment group), and GSH post-processing group (post-treatment group), with ten rates in each group. Animals in
pre-treatment group were injected 4% GSH 100 mg/kg intraperitoneally at 24th , 16th , 8th hour and 45th minute before surgery. Animals in post-treatment group were administrated GSH with the same dosage at 45th minute, 6th, 12th and 18th hour after surgery. Creatinine (Cr), urea nitrogen (BUN) level, the total superox-ide dismutase (T-SOD) activity, malondialdehyde (MDA) and nitric oxide (NO) levels in serum were measured at 24th hour after surgery. Histopathological changes were checked by H. E staining. Results Damage on kid-ney structure of animals in pre-treatment group was less than that in I/R group. There was little pathological change on kidney of those in pre-treatment group. Serum Cr, BUN, MDA and NO levels were all decreased but T-SOD activity increased in pre-treatment and post-treatment group when compared with those in I/R group (P <0.05), (P < 0.05). T - SOD activity in post-treatment group was higher than that in pre-treatment group (P <0.05). Conclusion GSH can protect rats against acute renal ischemia-reperfusion injury within 24 hours before and after kidney ischemia-reperfusion , especially after ischemia-reperfusion.
【总页数】4页(P1233-1236)
【作者】蔺艳;何涛;毛晓燕;甘仲霖;张莉;侯静
【作者单位】646000 四川省泸州市，西南医科大学肿瘤研究所;646000 四川省泸州市，西南医科大学肿瘤研究所;西南医科大学第一附属医院儿科，肾病内
科;646000 四川省泸州市，西南医科大学肿瘤研究所;646000 四川省泸州市，西南医科大学肿瘤研究所;西南医科大学第一附属医院儿科，肾病内科
【正文语种】中文
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