STZ性糖尿病性大鼠白内障的AQP0、AQP1下调表达及Ca2+内流参与机制

STZ性糖尿病性大鼠白内障的AQP0、AQP1下调表达及
Ca2+内流参与机制
沈烨宇;刘文斌
【期刊名称】《海南医学》
【年(卷),期】2014(000)022
【摘要】Objective To explore the involvement of AQP0/1 and Ca2+ influx in streptozotoxin (STZ)-in-duced diabetic cataract. Methods Forty male SD rats were divided into diabetic model group (n=20) and calcium channel blocker (nifedipine) group (n=20), while 20 normal rats were taken as control group. The diabetic model group was replicated by injection of streptozotoxin (STZ), and calcium channel blocker group was given 60 mg/kg nifedipine from the fifth week to the eighth week. The concentration of serum blood glucose and cholesterol was ana-lyzed. The expression of AQP0/1 and IL-6 were detected by Western blot. Results Compared with the control group, the expression of AQP0/1 were down-regulated and the expression of IL-6 was up-regulated in diabetic model group (P<0.01). Compared with diabetic model group, the abnormal expression of AQP0/1 and IL-6 was significantly alleviated in calcium channel blocker group (P<0.05). Conclusion High concentration of glycemia upregulated the expression of IL-6 which enhanced the downregulated expression of AQP0/1.%目的：分析钙内流及水通道蛋白AQP0/1在链脲佐菌素(STZ)诱导的糖尿病大鼠白内障发病中的参与机制，为相关
研究与临床治疗提供参考。

方法40只糖尿病大鼠随机分为糖尿病模型组(n=20)和药物干预组(钙通道阻滞剂硝苯地平组，n=20)，20只同批次相同饲养条件的大鼠作为正常对照组。

药物干预组大鼠第5周到8周持续给予钙通道阻滞剂硝苯地平
灌胃治疗(60 mg/kg)，共持续4周。

Western blot分析各组中AQP0/1及炎症
因子IL-6的表达。

结果与对照组比较，糖尿病模型组大鼠晶状体中AQP0/1表达下调(P<0.01)，IL-6表达上调(P<0.01)；与糖尿病模型组比较，钙通道阻滞剂可
以部分恢复AQP0/1的异常作用(P<0.05)，对IL-6也有一定的改善作用(P<0.05)。

结论高血糖诱发炎症因子IL-6上调表达，并进一步增强细胞钙内流及AQP0/1下调表达。

【总页数】3页(P3290-3292)
【作者】沈烨宇;刘文斌
【作者单位】南京明基医院眼科，江苏南京 210019;南京明基医院眼科，江苏南京 210019
【正文语种】中文
【中图分类】R-332
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