MMP-2、MMP-3和 MMP-9在胃癌患者中的表达及意义

MMP-2、MMP-3和 MMP-9在胃癌患者中的表达及意义杨云鹏;刘莉;李慧
【期刊名称】《局解手术学杂志》
【年(卷),期】2016(025)009
【摘要】目的：研究 MMP-2、MMP-3和 MMP-9在胃癌细胞转移中作用，进一步探讨 MMP-2、MMP-3和 MMP-9的临床意义。

方法体外培养 SGC-7901胃癌细胞和 GES-1胃上皮细胞，划痕损伤实验模型和 Transwell 小室模型法检测这2种细胞的迁移， ELISA 法检测培养液中 MMP-2、MMP-3和 MMP-9的含量。

免疫组化检测胃癌、胃炎患者和正常人 MMP-3的阳性率，ELISA 检测血清中 MMP-2、MMP-3和 MMP-9的含量。

结果细胞划痕实验和 Transwell 实验均显示，SGC-7901细胞迁移能力强于 GES-1；SGC-7901分泌的3种蛋白含量均高于 GES-1；免疫组化结果显示，胃癌患者 MMP-3的阳性率84．4％，高于胃炎患者和正常人。

其血清中 MMP-2、MMP-3和 MMP-9也明显高于胃炎患者和正常人。

结论胃癌细胞的转移能力与 MMP-2、MMP-3和 MMP-9的含量相关，3种蛋白可用于胃癌的早期诊断。

%Objecitive The research was in order to explore the roles of MMP-2,MMP-3 and MMP 9 which played in the gastric cancer cell metastasis,and make a further study of the clinical signifance.Methods Both SGC-7901 and GES-1 were cultured in vivo.The migra-tion were examined by Wound-healing and the Transwell assay.The expression of MMP-2,MMP-3 and MMP 9 in the medium were detected by ELISA.The positive rate of MMP-3 in the gastric cancer,gastritis and normal was used the IHC,and the serum of MMP-2,MMP-3 and MMP-9 were also
examined by ELISA.Results Both Wound-healing and Transwell assay showed that the migration of SGC-7901 cells was more quicker than the paring to GES-1,the MMP-2,MMP-3 and MMP-9 were higher in the SGC-7901.The immunohistochemical results showed that the positive rate of MMP-3 in the gastric cancer patients was 84.4%,it was higher than the latter.The serum levels of MMP-2 MMP-3 and MMP-9 were also significantly higher than others.Conclusion The migration of gastric cancer cells was depended on the MMP-2,MMP-3 and MMP-9.The 3 proteins can be used for the diagnosis of gastric cancer patients,and it also avail to make the judgment the prognosis of patients with gastric carcinoma.
【总页数】4页(P625-628)
【作者】杨云鹏;刘莉;李慧
【作者单位】成都大学附属医院病理科，四川成都610081;成都大学附属医院病理科，四川成都610081;成都大学附属医院病理科，四川成都610081
【正文语种】中文
【中图分类】R361+.3
【相关文献】
1.MMP-2和MMP-9在胃癌患者组织和血清中的表达及临床意义 [J], 常静;薛歆;刘爱中;于庭
2.MMP-2和MMP-3在非小细胞肺癌组织中的表达及意义 [J], 于学燕;王永刚
3.MMP-3、MMP-9及TIMP-1在急性心肌梗塞中的表达及其意义 [J], 沙洪芳;秦玲;李洋;张家颖
4.胃癌患者血清中MMP-2和MMP-9的临床意义研究 [J], 吴云;蔡钢
5.基质金属蛋白酶MMP-1、MMP-2、MMP-3在小儿胆道闭锁肝组织中的表达及意义 [J], 周雪鸿;冯启立;邢欣然;单振潮
因版权原因，仅展示原文概要，查看原文内容请购买。