七叶一枝花根茎和地上部分提取物镇痛抗炎作用研究

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文章编号：1001(880(2018)5(8%2(8

七叶一枝花根茎和地上部分提取物镇痛抗炎作用研究

丁立帅U，赵猛U，李燕敏#，陈两绵#，王智民#，王祝举#，高慧敏1!，李韶菁1!

1中国中医科学院中药研究所中药质量控制技术国家工程实验室，北京100700;2河南中医药大学药学院，郑州450008摘要:采用镇痛、抗炎实验对七叶一枝花根茎和地上部分提取物进行活性部位追踪。镇痛作用采用热板、热

刺痛和醋酸扭体实验进行评价，抗炎作用采用二甲苯致小鼠耳肿胀模型进行评价，比较总提物各部位及呋甾皂

苷、偏诺皂苷、薯蓣皂苷三类组分的抗炎作用强弱。结果表明七叶一枝花根茎提取物具有明显镇痛作用:在热

板实验中，仅高剂量组(2.4 g生药• k g1)表现出显著的镇痛作用，在热刺痛实验中，中、高剂量组（0.6g生药

• kg(，2.4g生药• k g1)表现出显著的镇痛作用，在醋酸扭体实验中，所有剂量组均表现出极显著的镇痛效果

并呈现剂量依赖性。抗炎作用评价中，总提物对二甲苯致小鼠耳肿胀抑制作用显著且表现出明显的剂量依赖

关系。地上部分总提物表现出与根茎相似的镇痛抗炎作用。大孔吸附树脂50%、70%及95%乙醇洗脱部位富

集的留体皂苷类成分是抗炎主要活性部位，其中呋留皂苷、偏诺皂苷和薯蓣皂苷三类组分均有显著的抗炎作

用，尤其以偏诺皂苷组的作用最强。本研究为建立与传统功效相关的重楼药材和饮片的质量评价体系的指标

成分选择提供了实验依据，同时，为七叶一枝花地上部分废弃资源的综合利用与开发提供了科学支撑。

关键词:七叶一枝花;镇痛;抗炎;呋留皂苷;偏诺皂苷;薯蓣皂苷

中图分类号：R285.5;Q946 文献标识码：A DOI ：10. 16333/j. 1001 -6880.2018. 5. 017 S t u d y o n t h e A n t i-n o c ic e p t iv e a n d A n t i-i n f l a m m a t o r y E f f e c t s o f T h e E x t r a c t o f A e r i a l P a r t a n d R h iz o m e o f P aris p o ly jjh ylla v a r.ch in e n sis

DING Li-sliuai^，2，ZHAO Meng1，2，LI Yan-min1，CHEN Liang-mian1，WANG Zhi-min1，

WANG Zhu-ju1，GAO Hui-min1*，LI Shao-jing1*

1Institute o f Chinese Materia M edica，China Academy o f Chinese Medical Sciences，National Engirwering

Laboratory fo r Quality Control Technolo^>y o f Chinese Herbal M edicine，Beijing，100700，C hina%

2 Cofeg^e o f Pharm acy，Henan University y f Chinese M edicine，Zhengzhoo 450008，China

A bstract ：The antinociceptive and a nti-inflammatory effects of tlie extract of aerial parts and rhizome of Paris polyphyfa

v ar. c hinensis were evaluated to find active fractions. The hot plate test，thermal radiation pain test and acetic acid-in­

duced mouse writliing test were used to assess the anti-nociceptive effect and xylene-induced mouse ear edema was con­

ducted to assess t he anti-inflammatory effect of the extract，fractions and sub-fractions ( furostanol saponin，pennogenin

saponin and dioscin saponin). The results showed that the rhizome extract displayed the significant anti-nociceptive

effects: the extract at the dose of 2. 4 g crude material • kg- showed significant anti-nociceptive effect in the hot plate

test，while the extract at the doses of both 0. 6 and 2. 4 g crude material • kg- displayed significant anti-nociceptive

effect in the t hermal radiation pain test. The extract at all doses of (0. 3，0. 6 and 2. 4 g crude material • kg- ) displayed

significant anti-nociceptive effect in a dose-dependent manner in the writhing test. The extract significantly inhibited the

mouse ear edema induced by xylene in a dose-dependent manner. The extract of aerial parts showed the similar anti-noci­

ceptive and anti-inflammatory effect as those of rhizome. The anti-inflammatory effect of aerial tributed to the steroidal saponins enriched in the fractions eluted by 50%，70% and 95%ethanol on macroporous resin

chromatography. All sub-fractions (furostanol，pennogenin and dioscin saponin) demonstrated the potent anti-inflamma­

tory effect，and among them，pennogenin saponins have the strongest activity. The present investigation idences for the development of quality evaluation system concerning the traditional e fficacy of Paridis 收稿日期:2017-08-17 接受日期:2018-02-7

基金项目：国有重点研发计划“中医药现代化研究”项目（2017YFC1701900);中医药行业科研专项（201507002);国家中药标准化项目（ZY BZH-Y-FJ-09);中央级公益性科研院所基本科研业务费专项（GH2017-03-03)

* 通信作者Tel:86-10-84014128 ;E-mail:hmgao@icmm. ac. cn;shaojingli2004@126. com