家兔腹主动脉球囊损伤动物模型的制作

家兔腹主动脉球囊损伤动物模型的制作
刘长青;尚小明;纪征;卢峰;李霞;赵庆霞
【期刊名称】《河北医药》
【年(卷),期】2013(35)22
【摘要】目的：探讨家兔腹主动脉球囊损伤模型的制作方法。

方法健康雄性新西
兰兔30只随机分成假手术组、2．5 mm球囊组和3．5 mm球囊组，每组10只。

除假手术组外分别采用2．5 mm ×15 mm和3．5 mm ×15 mm的球囊经股动
脉制作腹主动脉球囊损伤模型，喂养4周后比较制模时间、血管平均狭窄程度、
新生内膜层和中部平滑肌细胞层的平均厚度。

结果假手术组手术时间为（16±3）min，短于2．5 mm球囊组（29±4）min，短于3．5 mm球囊组（35±6）min，3组手术时间两两比较，差异有统计学意义（ P ＜0．05）。

血管平均狭窄程度同假手术组比较分别增加（22．1±3．5）％和（32．5±5．8）％，3组比
较差异统计学意义，3．5 mm球囊组狭窄重于2．5 mm球囊组，2．5 mm球
囊组重于假手术组。

内膜厚度3．5 mm球囊组为（0．07±0．03）mm大于2．5 mm球囊组（0．03±0．02）mm和假手术组（0．02±0．03）mm，差
异有统计学意义（P ＜0．05）。

中膜厚度3．5 mm球囊组为（0．16±0．03）mm大于假手术组（0．12±0．02）mm，差异有统计学意义（ P ＜0．05），
而与2．5 mm球囊组（0．14±0．02）mm比较差异无统计学意义（ P ＞0．05）。

结论采用直径为3．5 mm的球囊导管制作血管内膜损伤动物模型优于直径为2．5 mm的球囊导管。

%Objective To investigate the preparation method of sacculus injury model in abdominal aorta of rabbits.Methods 30 New Zealand rabbits were randomly divided into 3 groups:2.5mm sacculus
group ( n =10),3.5mm sacculus group ( n =10) and control group ( n
=10).Except for sham-operation group,the animal models of sacculus injury in abdominal aorta of rabbits were established by 2.5mm or 3.5mm sacculus injury .The time of making models , blood vessel average stenosis extent , average thickness of neogenesis intima and tunica media were detected and compared after 4-week feeding .Results The operation time in sham-operation group was (16 ±3)min,which was significantly shorter than (29 ±4) min in 2.5mm sacculus group and (35 ±6) min in 3.5mm sacculus group ( P <0.05).As compared with that in sham-operation group,the blood vessel average stenosis extent in 2.5mm sacculus group or 3.5mm sacculus group was increased by (22.1 ±3.5)% and (32.5
±5.8)%,respectively ( P <0.05),which in 3.5mm sacculus group was severer than that in 2.5mm sacculus group,moreover,which in 2.5mm sacculus group was severer than that in sham-operation group.The thickness of intima in 3.5mm sacculus group was(0.07 ±0.03)mm,which was significantly greater than (0.03 ±0.02)mm in 2.5mm sacculus group and (0.02 ±0.03) mm in sham-operation group ( P <0.05).The thickness of tunica media in 3.5mm sacculus group was(0.16 ±0.03)mm,which was significantly greater than (0.12 ±0.02)mm in sham-operation group ( P
<0.05),however,there was no significant difference in the thickness of tunica media between 3.5mm sacculus group and 2.5mm sacculus group ( P >0.05).Conclusion The 3.5mm sacculus catheter is superior to 2.5mm sacculus catheter in making the animal model with endangium injury .【总页数】3页(P3378-3380)
【作者】刘长青;尚小明;纪征;卢峰;李霞;赵庆霞
【作者单位】063000 河北省唐山市工人医院心内一科;063000 河北省唐山市工人医院心内一科;063000 河北省唐山市工人医院心内一科;063000 河北省唐山市工人医院心内一科;063000 河北省唐山市工人医院心内一科;063000 河北省唐山市工人医院心内一科
【正文语种】中文
【中图分类】R543.1
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