METHOD FOR PRODUCTION OF A THERAPEUTICALLY PURE GR

专利名称：METHOD FOR PRODUCTION OF A
THERAPEUTICALLY PURE GRANULOCYTE
MACROPHAGE - COLONY STIMULATING
FACTOR
发明人：KOMATH, Uma Devi, Zenotech Labs.
Ltd.,SAMADDAR, Mitali, Zenotech Labs.
Ltd.,LODHA, Sanjay, Zenotech Labs.
Ltd.,HAZARI, Venkateswar Rao, Zenotech
Labs. Ltd.,CHAKRAVARTHY, S. N., Zenotech
Labs. Ltd.,MOVVA, Lalitha, Zenotech Labs.
Ltd.,CHIGURUPATI, Jayaram, Zenotech Labs.
Ltd.
申请号：EP05732861.9
申请日：20050318
公开号：EP1833846A2
公开日：
20070919
专利内容由知识产权出版社提供
摘要：A simple and scaleable process is provided for obtaining recombinant human GM CSF expressed in microbial cells, secreting mature GM-CSF, culturing these cells and purifying the protein in good yield and of a therapeutic grade quality. The steps include cloning, fermentation and purification of the protein. The purified protein is got by a process that involves lysing the microorganism, extraction of the processed and unprocessed forms of GM-CSF from the membrane pellet and selectively purifying the processed form of the protein from the unprocessed form by a two-step
chromatography procedure. The purity of the processed form of GM-CSF (without signal peptide) obtained has endotoxin units, host cell proteins and host cell DNA within the specified compendial limits for therapeutic proteins.
申请人：Zenotech Laboratories Limited
地址：8-3-677 15 SKD Nagar, Srinagar Colony Hyderabad, Andhra Pradesh 500073 IN 国籍：IN
代理机构：Herzog, Martin
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