Research on Light Polarization FSO-OFDM System

合集下载

polarization

polarization

In this paper we report an ab initio study of the sponta-
neous polarization, piezoelectric constants, and dynamical
charges of the III-V nitride semiconductors AlN, GaN, and InN.1 This class of polarization-related properties is of obvious importance for the study of nitride-based piezodevices2
that the vector connecting the cation with the anion has
0163-1829/97/56͑16͒/10024͑4͒/$10.00
56 R10 024
© 1997 The American Physical Society
RAPID COMMUNICATIONS
In the absence of external fields, the total macroscopic
polarization P of a solid is the sum of the spontaneous polarization Peq in the equilibrium structure, and of the straininduced or piezoelectric polarization ␦P. In the linear regime, the piezoelectric polarization is related to the strain ⑀ by

LED研究热点与前沿的知识图谱分析―――基于SCIE中三种代表刊(精)

LED研究热点与前沿的知识图谱分析―――基于SCIE中三种代表刊(精)

LED 研究最活跃的国家。 从领域 H 指数来看,美国也 是 LED 领域研究成果最具有影响力的国家,其次是日 本。 中国无论是从载文量来看,还是从领域 H 指数来
看,都位居第五位。
表 3 高产国家和地区载文分布
排序 1 2 3 4 5 6 7 8 9 10 11 12
国家或地区 美国 USA 台湾 Taiwan 韩国 South Korea 日本 Japan 中国 Peoples R China 英国 UK 德国 Germany 加拿大 Canada 意大利 Italy 法国 France 新加坡 Singapore 瑞士 Switzerland
大洲 发文量 中心度 领域 H 指数
美洲 284 0. 59
8
亚洲 226 0. 37
5
亚洲 151 0. 26
5
亚洲 146 0. 25
6
亚洲 125 0. 21
4
欧洲 94 0. 28
4
欧洲 78 0. 20
3
美洲 30 0. 00
2
欧洲 29 0. 02
2
欧洲 25 0. 04
1
亚洲 21 0. 06
载文量 700 255 216 197 186
表 2 LED 领域前 5 种高被引期刊分布
排 序
期刊名称
被引 频次
IF
5年 期 IF
EF
1 APPLIED PHYSICS LETTERS
2049 3. 841 3. 863 0. 71882
2
IEEE PHOTONICS TERS
TECHNOLOGY
led研究热点与前沿的知识图谱分析基于scie中三种代表刊吴学雁艾丹祥张延林广东工业大学管理学院广州510520摘要选取webofscience平台上scie数据库中收录的led领域的3种国际代表性期刊19992011年间的论文为研究对象利用信息可视化软件citespace对引文数据和主题词数据进行分析和处理生成了led领域的共被引网络知识图谱和共现混合网络知识图谱

Performing Fluorescence Polarization Assays on the

Performing Fluorescence Polarization Assays on the

Multimode Detection Performing FluorescencePolarization Assays on theVICTOR NivoIntroductionFluorescence Polarization (FP) is a homogeneous assay formatthat is highly suitable for many applications from occasionalusage to high throughput screening, due to rather inexpensive reagents and its signal stability1. In FP assays, polarized light isused to determine the rotation capabilities of smallfluorescently labelled molecules. With this assay principle, onecan indirectly detect whether tracer molecules are bound to amuch larger molecule or are freely rotating in solution. Theseare rather complex interrelationships on the assay as well as on the device side compared to other homogeneous, plate reader compatible assays. Hence, for users, it is often difficult to set up an FP assay correctly.For this reason, we describe in this T echnical Note how to set up a Fluorescence Polarization assay on the VICTOR® Nivo™multimode plate reader and provide guidance for protocoloptimization. The VICTOR Nivo is a compact multimode plate reader that provides all detection modes which are routinelyused in drug discovery: Absorbance, Luminescence,Fluorescence Intensity, as well as options for Alpha, Time-Resolved Fluorescence and Fluorescence Polarization. Due toits intuitive control software and small footprint, the platereader fits easily in any lab.For research use only. Not for use in diagnostic procedures.As an example assay, the Predictor™ hERG Fluorescence Polarization Assay2 was used and its principle is shown in Figure 1, where the fluorescently labelled small moleculesof the Predictor™ hERG Tracer Red can either bind to the hERG channel protein in Predictor™ membrane fraction or can rotate freely.Blocking of the hERG potassium channel is known to be a potential off-target activity of drug candidates2,3, that can lead to life-threatening arrhythmias. For this reason, effects on the hERG channel are investigated early in the drug discovery process using various methodologies, one of them being the Fluorescence Polarization assay.VICTOR Nivo Multimode Plate Reader2Figure 2. Plate layout for the instrument setup run on the VICTOR Nivo.Instrument Setup Run for Predictor ™ hERG FP Assay The instrument setup run is a step used to optimize the FP measurement protocol specifically for the Predictor ™ hERGFluorescence Polarization Assay (Invitrogen, # PV5365) with regard to Z-height and G factor . For this experiment, a set of assay controls is needed: Buffer Blank, Assay Blank, Free tracer control, Negative control and Positive control. The controls were prepared according to the assay manual 5 and were transferred in triplicates to a black 384-well assay plate (PerkinElmer , ProxiPlate # 6008260 or OptiPlate # 6007270) at a volume of 20 ul/well (Figure 2).1. Selection of FiltersIn order to set up a FP measurement protocol on the VICTOR Nivo, three filters and a dichroic mirror are needed: a 530/30 nm excitation filter, two 580/20 nm emission filters and a 565 nm dichroic mirror. Alternatively, a 50/50 beam splitter can be used, but assay performance may be impaired. Dedicated polarization filters are not needed as the necessary polarizing components are already located inside the plate reader, if the instrument is equipped with FP technology.2. Z-focus Height OptimizationUsing a free tracer control well (reference polarization control), the Z-focus height optimization was demonstrated for a 384-well ProxiPlate and 384-well OptiPlate. A FP Z-focus scan protocol was set up (excitation at 530 nm, emission at 580 nm) with 20 scan points between 0 and 20 mm (Figure 3). The emission values (either S or P) were plotted in the VICTOR Nivo control software (Figure 4). The plate specific optimal Z-focus height was determined at the emissionintensity maximum. For future FP measurements, this Z-focus height was transferred to the FP endpoint protocol ofthe control software.Figure 1. Assay Principle. If polarized light excites Tracer Red bound to the hERG channel protein, the emission light remains polarized, because the tracer-channel-complex rotates slowly during fluorescent lifetime. In contrast, inhibiting compounds in the ion channel block Tracer Red from binding. In case Tracer Red is replaced in the ion channel by a compound, it rotates quickly during fluorescent lifetime due to its small size. This is leading to highly depolarized emission light, which is detected by the instrument not only in S, but also in P orientation.3Figure 3. Z-focus scan protocol for the plate specific optimization of the Z-focus height.3. G Factor CalculationThe G factor is a correction factor used to compensate for differences in parallel and perpendicular optical components of the measurement device. Calculating the G factor isrecommended, if the true polarization should be determined. Here, it was calculated using the free tracer control wells. In the Predictor ™ hERG FP Assay, this reference control has a known value of 50 mP 5. As a first step, the assay plate was measured once with the FP endpoint protocol including a G factor of 1. The S and P channel results were then used to calculate the G factor using Microsoft Excel according to the following formula:G =S*(1 – )mP (T racer )1000P*(1 + )mP (T racer )1000If the literature polarization value is not known for the used fluorophore, the relative change of polarization values (ΔmP) upon treatment can be plotted to create dose-responsecurves. For this, the G factor does not need to be adjusted and can be kept at 1. T o calculate ΔmP , all resulting mP values of the curve are normalized to an assay relevant sample showing low polarization values such as the free tracer control, positive control or even the lowest compound concentration in this example.As a rule of thumb, G is usually 0.8 < G < 1.2. The assayspecific calculated G factor was inserted in the FP endpoint protocol of the control software and the measurement of the assay plate repeated. The G factor was determined correctly, if the known mP value of the reference control (here 50 mP , see above) is obtained as a result.4Figure 5. Final VICTOR Nivo measurement protocol for the Predictor™ hERG FP assay shown here for 384-well ProxiPlates.Compound Testing in the Predictor ™ hERG FP AssayThe known hERG channel inhibitors Astemizole (Cayman chemical, #16967) and T erfenadine (Cayman chemical, #20305) were tested in 16-point dose response curves in a concentration range of 3.3 µM - 0.2 pM in the FP assay. T o allow data correction in case of unspecific compound effects, both compounds were also tested in the presence of a saturating concentration of the inhibitor E-4031 (30 µM). The plate layout is shown in Figure 6.First, the test compounds were dissolved in DMSO and a 3-fold dilution series was prepared. Afterwards, all samples were diluted 1:25 in assay buffer. Compounds were transferred to the assay plate at a volume of 5 µl/well. The tracer was diluted to 4 nM and 5 µl/well were transferred into the assay. Finally, 10 µl/well of the Predictor™ hERG Membrane were dispensed into a ProxiPlate (PerkinElmer, # 6008260). After 2 hours ofincubation at room temperature, the assay plate was placed in the VICTOR Nivo to run the FP protocol with the measurementsettings shown in Figure 5.Figure 4. Z-focus height optimization was demonstrated in PerkinElmer OptiPlate and ProxiPlate using a FP Z-focus scan protocol (excitation at 530 nm, emission at 580 nm) with 20 scan points between 0 and 20 mm. The emission intensity maximum (red intersecting lines) was determined directly in the VICTOR Nivo software.5Figure 6. Plate layout for Compound Profiling in the Predictor ™ hERG assay. The compounds Astemizole and T erfenadine were tested in 16-point dose response (3.3 µM - 0.2 pM, triplicates per concentration) in the presence and absence of the inhibitor E-4031.ResultsAfter optimizing the FP protocol on the VICTOR Nivo, it was used to measure the assay plate containing controls. As shown in Figure 7, the free tracer control results in 50 mP on average, showing that the G factor has been optimized correctly using the literature value 5. Nevertheless, the actual assay window is the span between the negative (tracer and membrane) and positive control (tracer, membrane and 30 µM E-4031) in these experiments ~100 mP . Comparable results were obtained in the OptiPlate and ProxiPlate at a volume of 20 µl (data not shown). In addition, it can be helpful to look not only at the mP results but also to calculate the total intensity with the formula 2*P+S. For example, background signal (assay blank and buffer blank) is often highly polarized, but the intensities are actually very low. T aking the total intensity into account during data analysis can therefore helpavoid misinterpretation of results.Figure 7. Resulting mP values (left) and total intensity (right) of assay controls in ProxiPlate after protocol optimization on the VICTOR Nivo. For each sample, the mean and standard deviation of three wells are shown.In a subsequent experiment, the known inhibitors T erfenadine and Astemizole were tested in dose response in the FP assay, results are shown in Figure 8. The FP signal was detected 2 hours after incubation. Assay statistics for the two independent experiments are summarized in T able 1. For the calculations,16 positive control wells and 16 negative control wells were used. The Z prime values of 0.73 and 0.87 indicate a robust assayperformance for both experiments.Figure 8. The compounds Astemizole and Terfenadine were tested in dose response experiments. The following IC 50 values were determined: IC 50 (Astemizole)= 0.97 nM and IC 50 (Terfenadine)= 2.8 nM. For comparison, the assay manual 5 reports an IC 50 value of 1.9 nM for Astemizole. For each data point, the mean and standard deviation of three wells are shown.For a complete listing of our global offices, visit /ContactUsCopyright ©2021, PerkinElmer, Inc. All rights reserved. PerkinElmer ® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.211120 (145315) PKIPerkinElmer, Inc. 940 Winter StreetWaltham, MA 02451 USA P: (800) 762-4000 or (+1) 203-925-4602ConclusionWe demonstrated the steps for FP protocol setup and optimization on the VICTOR Nivo and used the established measurement protocol for testing hERG inhibitors in dose response in two independent experiments. Using the protocol optimization steps described in this technical note, VICTOR Nivo’s simple and flexible software enables users to quickly optimize FP assays. Software features such as the graph view for Z-focus scans and the applied G factor make it easy for users to determine the correctmeasurement height and to directly export the polarization values. Also, the innovative filter wheel with its built-in polarizingcomponents makes it possible to use any Fluorescence Intensity filter combination for FP assays. No dedicated polarization filters are needed, only a second identical emission filter is required. In summary, this demonstrates that its ease of use of FP assays is a valuable addition to the VICTOR Nivo, along with its standard detection technologies.References1. Lea WA, Simeonov A. Fluorescence Polarization assays in small molecule screening. Expert Opin Drug Discov. 2011;6(1):17-32. doi:10.1517/17460441.2011.5373222. Piper DR, Duff SR, Eliason HC, et al. Development of the predictor hERG Fluorescence Polarization assay using a membrane protein enrichment approach. Assay Drug Dev T echnol. 2008;6(2):213-223. doi:10.1089/adt.2008.1373. Birgit Priest, Ian M. Bell & Maria Garcia (2008) Role of hERG potassium channel assays in drug development, Channels, 2:2, 87-93, DOI: 10.4161/chan.2.2.60044. Dierk Thomas, Christoph Karle & Johann Kiehn (2004) Modulation of hERG potassium channel function by drug action, Annals of Medicine, 36:sup1, 41-46, DOI: 10.1080/174313804100325805. Predictor hERG Assay Manual (Rev. date: 28 October 2009, https:///order/catalog/product/PV5365#/PV5365)。

拉曼原理及特点和应用-RENISHAW

拉曼原理及特点和应用-RENISHAW
非弹性碰撞;方向 改变且有能量交换;
激发虚态
h(0 - )
E1 + h0
E0 + h0 h0
h0 h0
h0 +
E1
V=1
E0 Rayleigh散射 V=0 Raman散射 h
E0基态, E1振动激发态; E0 + h0 , E1 + h0 激发虚态; 获得能量后,跃迁到激发虚态.
apply innovation
• 1992年世界上首先发明新型显微共焦拉曼系统
最初的测头及 专利证书
David McMurtry爵士 董事长兼首席执行官
John Deer 副董事长
apply innovation
遍及全球的子公司和办事处
子公司 美国 (1981) 爱尔兰 (1981) 日本 (1982) 德国 (1986) 法国 (1988) 意大利 (1989) 西班牙 (1991) 瑞士 (1991) 香港 (1993) 巴西 (1996) 韩国 (1999) 印度 (2000) 比利时 (2000) 澳大利亚 (2001) 荷兰 (2001) 斯洛维尼亚 (2001) 捷克 (2002) 波兰 (2002) 俄罗斯 (2002) 奥地利 (2002) 瑞典 (2003) 加拿大 (2003) 以色列 (2003)
apply innovation
连续扫描专利技术 - 全光谱范围一次扫描,无接谱
apply innovation
inVia 系统特色-新型共焦专利技术
新型共焦技术
-高通光效率,操作简便,性能稳定
apply innovation
传统共焦技术 - 机械针孔
apply innovation
最新的显微共焦系统专利技术

荧光光谱分析方法及原理

荧光光谱分析方法及原理
EMISSlON LlFETlMES
Emission lifetimes of absorption, fluorescence, and phosphorescence at the equilibrium internuclear distance of the ground state.
仪器结构
某些物质被一定波长的光照射时,会在一定时间内发射出波长比入射光长的光,如果这个时间比较短,这种光就称为荧光。荧光由一种能发荧光的矿物 萤石(fluospar)而得名。 我们这里要介绍的荧光,是指物质在吸收紫外光和可见光后发出的波长较长的紫外荧光或可见荧光。 除了紫外光和可见光可能激发荧光外,其它的光如红外光、X射线也可能激发出荧光,因此除紫外荧光或可见荧光外,还有红外荧光、X射线荧光等。
Schematic diagram of a double-beam (ratiometric) filter fluorometer.
Filter fluorometers are suitable for quantitative analysis applications in which spectral scanning and high resolution are not required. Filters transmit more light and cost less than monochromators, thereby providing better detection limits with less expensive instrumentationque for detecting biological materials
荧光光谱灵敏度高的原因
荧光辐射的波长比激发光波长长,测量到的荧光频率与入射光的频率不同; 荧光在各个方向上都有发射,因此可以在与入射光成直角的方向上检测; 这样,荧光不受来自激发光的本底的干扰,灵敏度大大高于紫外-可见吸收光谱,测量用的样品量很少,且测量方法简便。

LPS与ATP共同诱导小鼠原代腹腔巨噬细胞焦亡模型的建立

LPS与ATP共同诱导小鼠原代腹腔巨噬细胞焦亡模型的建立

LPS 与ATP 共同诱导小鼠原代腹腔巨噬细胞焦亡模型的建立①刘慧玲 吴传新② 龙贤梨 李丽 李飞 郭晖 孙航(重庆医科大学附属第二医院病毒性肝炎研究所,重庆 400010)中图分类号 R392.1 文献标志码 A 文章编号 1000-484X (2023)10-2028-06[摘要] 目的:探索脂多糖(LPS )和三磷酸腺苷(ATP )共同诱导小鼠原代腹腔巨噬细胞焦亡模型的最佳条件。

方法:采用流式细胞仪F4/80和CD -11b 染色检测巨噬细胞纯度,Annexin V -PE/7-AAD 双染色法筛选出LPS 和ATP 共同诱导细胞焦亡的最适浓度及时间。

巨噬细胞随机分为control 组、LPS 组、ATP 组和LPS+ATP 组;Western blot 检测GSDMD 、caspase -1、caspase -11、NLRP3、ASC 、pro -IL -1β、pro -IL -18和HMGB1蛋白表达水平;ELISA 检测培养上清中IL -1β和TNF -α表达水平;透射电镜(TEM )和扫描电镜(SEM )观察巨噬细胞焦亡形态。

结果:巨噬细胞的纯度达到90%;500 ng/ml LPS 24 h+5 mmol/L ATP 4 h 为诱导巨噬细胞焦亡的最佳组合方式;LPS+ATP 组的GSDMD 、caspase -1、caspase -11、NLRP3、ASC 、pro -IL -1β、pro -IL -18和HMGB1的蛋白表达量明显高于对照组(P <0.05);培养上清中IL -1β和TNF -α表达量显著高于对照组(P <0.05);电镜下可观察到明显的焦亡特征。

结论:成功建立了LPS 和ATP 共同诱导小鼠原代腹腔巨噬细胞的焦亡模型,为深入探讨免疫细胞焦亡的分子机制提供了稳定的细胞模型。

[关键词] LPS ;ATP ;细胞焦亡;原代腹腔巨噬细胞;脓毒症Establishment of pyroptosis model on primary peritoneal macrophages induced by LPS and ATPLIU Huiling , WU Chuanxin , LONG Xianli , LI Li , LI Fei , GUO Hui , SUN Hang. Institute for Viral Hepatitis , the Second Affiliated Hospital , Chongqing Medical University , Chongqing 400010, China[Abstract ] Objective :To explore optimal condition of a model of pyroptosis on primary peritoneal macrophages induced by thelipopolysaccharide (LPS ) and adenosine triphosphate (ATP ). Methods :Purity of macrophages was detected by flow cytometric with F4/80 and CD11-b , and Annexin V -PE/7-AAD double staining was used to detect pyroptosis cell for screening the optimum concentra‑tion and time of pyroptotic cells induced by LPS and ATP. Macrophages were randomly divided into control group , LPS group , ATP group and LPS+ATP group. Expressions of GSDMD , caspase -1, caspase -11, NLRP3, ASC , pro -IL -1β, pro -IL -18 and HMGB1 proteins were detected by Western blot. Levels of IL -1β and TNF -α in culture supernatant were measured by ELISA. Structure of pyroptosis macrophages was observed by transmission electron microscope (TEM ) and scan electron microscope (SEM ). Results :Purity of primary peritoneal macrophages could be 90%; 500 ng/ml LPS 24 h and 5 mmol/L ATP 4 h was the optimal combination of inducing macrophages pyroptosis. Compared with control group , LPS and ATP group had significantly increased protein expressions of GSDMD , caspase -1, caspase -11, NLRP3, ASC , pro -IL -1β, pro -IL -18 and HMGB1 (P <0.05), and levels of IL -1β and TNF -α in culture supernatant were significantly higher than that in control group (P <0.05); structure of pyroptosis macrophages could be obviously observed by TEM and SEM. Conclusion :Pyroptosis model of primary peritoneal macrophages induced by LPS and ATP is successfully established , whichprovides a cell model for exploring the molecular mechanism of pyroptosis on immune cells in the future.[Key words ] LPS ;ATP ;Pyroptosis ;Primary peritoneal macrophages ;Sepsis细胞焦亡是一种依赖半胱天冬蛋白酶(caspase -1/-4/-5/-11)活化的炎症细胞死亡方式,其形态介于细胞凋亡和细胞坏死之间,且细胞焦亡的发生机制和调控机制与凋亡和坏死大不相同[1]。

2024届高三英语基础写作:邀请外教参加重阳节活动+课件

2024届高三英语基础写作:邀请外教参加重阳节活动+课件

13. What does the underlined phrase “pored over” in paragraph 3 probably mean?
A. Copied.
ቤተ መጻሕፍቲ ባይዱ
B. Covered.
C. Studied.
D. Borrowed.
2024届新高三摸底联考
英语
第二部分 语言运用(共两节,满分30分)
We deal in hardware but not software. 我们只经营硬件而不经营软件。 2) (贬义) concern oneself with sth; indulge in sth 忙於某事物; 沉溺於某事物 deal in gossip
share (in) sth. 分摊或分享某事物; 参与某事物 I will share (in) the cost with you. 我愿与你分摊费用。 She shares (in) my troubles as well as my joys. 她与我同甘共苦。
2024届新高三摸底联考
英语
第二节 (共10小题;每小题1.5分,满分15分)
阅读下面短文,在空白处填入1个适当的单词或括号内单词的正确形式。
Movies could serve as a valuable “classroom” for children to explore into the
culture and history of their own country. A new book 36. collecting .(collect) 20
take in
He was homeless, so we took him in. 他无家可归, 我们就收留了他。 She took me in completely with her story. 她用谎话把我完全蒙蔽了。 Don’t be taken in by his charming manner; he’s completely ruthless. 不要被他那副讨人喜欢的外表所迷惑, 其实他冷酷无情。 Fish take in oxygen through their gills. 鱼通过鳃摄取氧气。 He took in every detail of her appearance. 他端详了她一番。 He took in the scene at a glance. 他看了一眼那里的景色。 I hope you’re taking in what I’m saying. 我希望你能听得进去我说的话。

光子的偏振特征及应用-巩龙延

光子的偏振特征及应用-巩龙延
E. Wave plates
38
Polarization Devices
E. Wave plates
39
Polarization Devices
E. Wave plates
40
Polarization Devices
F. Beam splitters
41
Polarization Devices
12
Polarization of light
• A2. Linearly polarized light
13
Polarization of light
• A3. Circular Polarized light
14
Polarization of light
• A4. Poincaré sphere
•circularly polarized
Note: From the perspective of polarization, paraxial waves may be approximated by plane waves and described by a single polarization ellipse (or circle or line).
F. Beam splitters
42
Polarization Devices
43
44
19Байду номын сангаас
Polarization of light
• B3. Matrix representation of polarization devices
20
Polarization of light
• B3. Matrix representation of polarization devices • Linear polarizers

紫外荧光光谱法英语

紫外荧光光谱法英语

紫外荧光光谱法英语Ultraviolet Fluorescence Spectroscopy.Ultraviolet fluorescence spectroscopy is an analytical technique that employs the fluorescence emitted by molecules excited by ultraviolet (UV) light to characterize chemical species. This method has found widespread applications in various fields, including chemistry, biochemistry, pharmacology, and environmental science.Principles of UV Fluorescence Spectroscopy.The principle of UV fluorescence spectroscopy lies in the absorption of UV light by molecules, which then emit light at longer wavelengths, known as fluorescence. This emission occurs when the absorbed energy causes electronsin the molecules to transition from a lower energy state to an excited state. As the electrons relax back to the lower energy state, they emit radiation in the form of light. The wavelength and intensity of this emitted light arecharacteristic of the specific molecular structure and can be used for identification and quantification.Instrumentation.UV fluorescence spectroscopy requires specialized instrumentation, primarily a UV-Vis spectrophotometer with a fluorescence detector. These instruments typically consist of a light source, a monochromator to select a specific wavelength of UV light, a sample compartment, and a detector to measure the emitted fluorescence. Modern spectrophotometers often incorporate advanced features such as multi-wavelength excitation and emission scanning, which provide richer spectral information.Applications of UV Fluorescence Spectroscopy.1. Biochemical Analysis: UV fluorescence spectroscopyis widely used in biochemistry to study protein-ligand interactions, protein conformational changes, and nucleic acid structure. Fluorescent probes can be attached to specific sites on proteins or nucleic acids, allowing theirbehavior to be monitored under different conditions.2. Drug Discovery and Pharmacology: This technique is employed in drug discovery to screen potential drugs for their binding affinity to biological targets. By monitoring the changes in fluorescence upon drug binding, researchers can assess the affinity and selectivity of drugs.3. Environmental Science: UV fluorescence spectroscopy has been used to monitor pollutants in water and air. Fluorescent tracers can be used to trace the fate and transport of pollutants, providing insights into environmental contamination and remediation.4. Materials Science: In materials science, UV fluorescence spectroscopy is used to study the optical properties of materials, such as quantum dots and fluorescent dyes. This technique can provide information about the energy levels and electronic states of these materials, which is crucial for their applications in optoelectronic devices.Advantages and Limitations.Advantages:High Sensitivity: UV fluorescence spectroscopy can detect very low concentrations of fluorescent species, making it suitable for trace analysis.Selectivity: By choosing specific excitation and emission wavelengths, UV fluorescence spectroscopy can provide information about specific components in complex mixtures.Non-Destructive: This technique does not require the destruction of samples, allowing multiple measurements to be performed on the same sample.Limitations:Fluorescent Probe Dependence: The application of UV fluorescence spectroscopy often relies on the availability of suitable fluorescent probes or dyes. Not all moleculesexhibit strong fluorescence, limiting the scope of this technique.Interference from Background Fluorescence: The presence of background fluorescence from the sample matrixor solvents can interfere with the measurement, affecting the accuracy and reliability of results.Instrument Cost and Maintenance: Specialized UV-Vis spectrophotometers with fluorescence detection capabilities can be costly, and regular maintenance is required toensure accurate measurements.Conclusion.UV fluorescence spectroscopy is a powerful analytical tool that has found widespread applications in various fields. Its ability to provide sensitive and selective information about molecular structure and interactions has made it a valuable resource for researchers in biochemistry, pharmacology, environmental science, and materials science. Despite its limitations, UV fluorescence spectroscopycontinues to evolve and improve, providing new insights into the behavior and properties of chemical species.。

Flourence polarization(荧光偏振)

Flourence polarization(荧光偏振)

I0 Polarizer Unpolarized (random) light
I = I0/2 Linear polarized light
Common polarizers:
• double refracting (birefrigent) calcite (CaCO3) crystals- which refract components of light polarized in two perpendicular planes under different angles • filters, which effectively absorb one plane of polarization (e.g., Polaroid type-H sheets based on stretched polyvinyl alcohol impregnated with iodine)
Anisotropy is preferred because it contains the total intensity IT
Anisotropy
Polarization in a fluorescence experiment
Z
Ill I Ill I r IT Ill 2I
r(t ) r0 exp( t )
Where is the rotational correlation time (Debye rotational relaxation time) which is the time for a given orientation to rotate through an angle given by the arccos e-1 (68.42o). For a spherical molecule:

principles of Physics--23exercises(optics)

principles of Physics--23exercises(optics)


1
(c)
d S2
1 2
L
O
Example 4: S1 and S2 are two coherent light sources. S1P=S2P. The light 1 goes through the glass of width t and index of refraction n1. The light 2 goes through the glass of width t and index of refraction n2. Find the optical path difference at point P.
polarization of the incident wave
3. Polarization by reflection and refraction 1). The incident angle i is any angle


n1 n2
i i



Partially polarized light
n2=n3
Example 3: In Young’s double-slit experiment, the glass of index of refraction n moves up. The interference fringes (a) move down. The distance between two adjacent fringes becomes bigger. (b) move up. The distance between two adjacent fringes becomes smaller. (c) move down. The distance between two adjacent fringes does not change. (d) move up. The distance between two adjacent fringes does not change. S

FluorescencePola...

FluorescencePola...

Fluorescence Polarization (FP)Ewald TerpetschnigISS Inc.PrinciplesFluorescence polarization (FP) measurements are based on the assessment of the rotational motions of species. FP can be considered a competition between the molecular motion and the lifetime of fluorophores in solution. If linear polarized light is used to excite an ensemble of fluorophores only those fluorophores, aligned with the plane of polarization will be excited. There are 2 scenarios for the emission.Provided the fluorescence lifetime of the excited fluorescent probe is much longer than the rotational correlation time θ of the molecule it is bound to (τfl >> θrot) (θ is a parameter that describes how fast a molecule tumbles in solution), the molecules will randomize in solution during the process of emission, and, as a result, the emitted light of the fluorescent probe will be depolarized. If the fluorescence lifetime of the fluorophore is much shorter than the rotational correlation time θ (τfl << θrot) the excited molecules will stay aligned during the process of emission and as a result the emission will be polarized.Figure 1. Relationship between fluorescence lifetime τ and rotational correlation time θ. Dependence of Fluorescence Polarization on Molecular Mobility [1]The fluorescence polarization (P) of a labeled macromolecule depends on the fluorescence lifetime (τ) and the rotational correlation time (θ)where P0 is the polarization observed in the absence of rotational diffusion. The effect of the molecular weight on the polarization values can be seen from an alternative form of the above equation:where k is the Boltzman constant, T is the absolute temperature, η the viscosity and V the molecular volume [2]. The molecular volume of the protein is related to the molecular weight (MW) and the rotational correlation time as given bywhere R is the ideal gas constant, v is the specific volume of the protein and h is the hydration, typically 0.2 g H2O per gram of protein. Generally, the observed correlation times are about two-fold longer than calculated for an anhydrous sphere due to the effects of hydration and the non-spherical shapes of most proteins. Hence, in aqueous solution at 20°C (η = 1 cP) one can expecta protein such as HSA (MW ~ 65,000, with h = 1.9) to display a rotational correlation time (θ) near50 ns.The measurement of fluorescence polarization is relatively straight-forward (Figure 2). In a typical experiment the sample containing the fluorescent probe is excited with linear polarized light and the vertical and horizontal components of the intensity of the emitted light are measured and the polarization (P) or anisotropy (r) are calculated using the following equations:Polarization (P) = (I v - I h) / (I v+ I h)Anisotropy (r) = (I v - I h) / (I v+ 2I h)where I v is the intensity parallel to the excitation plane and I h is the emission perpendicular to the excitation plane. They are interchangeable quantities and only differ in their normalization. Polarization P ranges from –0.33 to +0.5 while the range for anisotropy r is –0.25 to +0.4.P = 3 r / 2 + rr = 2 P / 3 - PFigure 2. Schematic drawing for the measurement of fluorescence polarization.Tracers for Polarization AssaysTracers used in fluorescence polarization assays include peptides, drugs, antibiotics etc. and they are typically synthesized by the reaction of a fluorescent dye with a reactive derivative of the analyte.Linker chemistries can have an impact on the fluorescence polarization. While tracers with short linkers between the fluorophore and the labeled molecule minimize the “propeller-effect”, a too short linker can affect the binding affinity of the tracer [3].Typical fluorophores used in FP are fluorescein and rhodamines. BODIPY dyes have longer excited-state lifetimes than fluorescein and rhodamine dyes, making their fluorescence polarization sensitive to binding interactions over a larger molecular weight range [4].A limitation of current fluorescence polarization immunoassays (FPIs) is that they are useful only for measurement of low molecular weight antigens. This limitation is the result of the use of fluorophores, such as fluorescein, which display lifetimes near 4 ns. An FPI requires that the emission from the unbound labeled antigen be depolarized, so that an increase in polarization may be observed upon binding to antibody. For depolarization to occur the antigen must display a rotational correlation time much shorter than 4 ns, which limits the FPI to antigens with molecular weight less than several thousand Daltons.A class of dyes that have been shown to combine long lifetime and high polarization are the metal-ligand complexes of Ru, Os and Re. These labels have lifetimes in the range of a few hundred ns to microseconds and would therefore allow measurement of higher molecular weight antigens but the strong propeller effect of the MLC when labeled to proteins other than HSA has limited their use as labels for high molecular weight analytes [2].Figure 3. Excitation polarization spectra of Ru-metal ligand complexes in solution at -55°C.ApplicationsFluorescence polarization measurements have been used in analytical and clinical chemistry [5,6] and as a biophysical research tool for studying membrane lipid mobility [7], domain motions in proteins, and interactions at the molecular level [8]. Fluorescence polarization based immunoassays are also extensively utilized for clinical diagnostics [9-11]. FP has the advantage that it requires only one labeled species for the assay (unlike energy-transfer based read outs that require two labeled species) and thus FP has become a very popular read out format for HTS (12-17). Many of these assays are based on the use of antibodies that provide the specificity needed to selectively detect a wide variety of antigens.An example for a homogeneous binding assay based on FP is shown below. Any material that enables a mass-increase of the labeled species can replace antibodies. In the IMAP assay™ (Molecular Devices) the high affinity of trivalent metal-ions to phosphate is utilized to generate the FP read-out [18].Figure 4. IMAPTM homogeneous binding assay for kinases and phosphatases.Books and Book Chapters related to Fluorescence Polarization1. Schulman, S.G. (Ed.) (1985). Molecular Luminescence Spectroscopy. Methods and Applications: Part1, J. Wiley & Sons, New York.2. Ichinose, N., Schwedt, G., Schnepel, F.M. and Adachi, K. (Eds.) (1987). Fluorometric Analysis inBiomedical Chemistry, J. Wiley & Sons, New York.3. Van Dyke, K. and Van Dyke, R. (Eds.) (1990). Luminescence Immunoassay and MolecularApplications, CRC Press, Boca Raton, FL.4. Lakowicz, J.R. (1999). Principles of Fluorescence Spectroscopy, 2nd Edition, Kluwer Academic/PlenumPublishers, New York.5. Steiner, R. F. (1991). Fluorescence anisotropy: theory and applications. In Topics in FluorescenceSpectroscopy. Vol. 2. Principles. Lakowicz, J.R. (Ed.) Plenum Press, New York.References1. Weber, G. in Hercules, D.M. (1966) Fluorescence and Phosphorescence Analysis. Principles andApplications, Interscience Publishers (J. Wiley & Sons), New York, pp. 217-240.2. Szmacinski, H., Terpetschnig, E. and Lakowicz, J.R. “Synthesis and Evaluation of Ru-complexes asAnisotropy Probes for Protein Hydrodynamics and Immunoassays of High-Molecular-Weight Antigens”.Biophysical Chemistry 62, 109-120 (1996).3. Huang. “Fluorescence Polarization Competition Assay: The Range of Resolvable Inhibitor Potency IsLimited by the Affinity of the Fluorescent Ligand”. J Biomol Screen. 8, 34-38 (2003).4. Schade SZ, Jolley ME, Sarauer BJ, Simonson LG. “BODIPY-alpha-casein, a pH-independent proteinsubstrate for protease assays using fluorescence polarization.” Anal Biochem. 243, 1-7 (1996).5. Jameson, D.M. and Seifried, S.E. “Quantification of Protein-Protein Interactions Using FluorescencePolarization”. Methods 19,222-233 (1999).6. Van Dyke, K. and Van Dyke, R. (Eds.) (1990) Luminescence Immunoassay and Molecular Applications,CRC Press, Boca Raton, FL. b) Jameson D.M. and Croney J.C. “Fluorescence Polarization: Past, Present and Future”. Combinatorial Chemistry & High Throughput Screening 6 (3), 167-176 (2003).7. Yu W., So PTC, French T, and Gratton E. Fluorescence generalized polarization of cell membranes-atwo photon scanning microscopy approach. Biophys. J. 70, 626-636 (1996).8. LeTilly V, and Royer CA. (1993), Fluorescence Anisotropy Assays Implicate Protein-Protein Interactionsin Regulating trp Repressor DNA Binding, Biochemistry 32, 7753-7758.9. Y and Potter JM. “Fluorescence polarization immunoassay and HPLC assays compared for measuringmonoethylglycinexylidide in liver-transplant patients”. Clinical Chemistry 38, 2426-2430 (1992).10. Bittman R and Fischkoff SA. “Fluorescence Studies of the Binding of the Polyene Antibiotics Filipin III,Amphotericin B, Nystatin, and Lagosin to Cholesterol”. PNAS 69 (12), 3795-3799 (1972).11. B, Verbesselt R, Scharpe S, Verkerk R, Lambert WE, Van Liedekerke B, De Leenheer A. “Comparisonof cyclosporin A measurement in whole blood by six different methods”. J. Clin Chem Clin Biochem.28(1):53-7 (1990).12. Parker GJ, Law TL, Lenoch FJ, Bolger RE. “Development of high throughput screening assays usingfluorescence polarization: nuclear receptor-ligand-binding and kinase/phosphatase assays”. J Biomol Screen 5, 77-88 (2000).13. Kim et al. “Development of a Fluorescence Polarization Assay for the Molecular Chaperone Hsp90”. JBiomol Screen 9, 375-381 (2004).14. Banks P, Gosselin M, Prystay L. “Fluorescence polarization assays for high throughput Screening of G-protein coupled receptors”. J.Biomol. Screen. 5, 159–167 (2000).15. Turek TC, Small EC, Bryant RW et al. “Development and validation of a competitive AKTserine/threonine kinase fluorescence polarization assay using product-specific antiphospho-serine antibody”. Anal. Biochem. 299, 45–53 (2001).16. Fowler A, Swift D, Longman E et al. “An evaluation of fluorescence polarization and lifetimediscriminated polarization for high-throughput screening of serine/threonine kinases”. Anal.Biochem.308, 223–231 (2002).17. Lu Z, Yin Z, James L et al. “Development of a fluorescence polarization bead-based coupled assay totarget different activity/conformation states of a protein kinase”. J. Biomol. Screen. 9, 309–321 (2004).18. Zaman G.J.R., Garritsen A. de Boer T., van Boeckel C.A.A. “Fluorescence Assays for High-ThroughputScreening of Protein Kinases”. Combinatorial Chemistry & High Throughput Screening 6 (4), 313-320。

Polarization of Light(I)

Polarization of Light(I)

1-2 Circular Polarization

Circularly polarized light consists of two perpendicular EM plane waves of equal amplitude and 90° difference in phase.
Right-Circularly Polarized Light
(1)Right-circularly polarized light: toward you appears to be rotating clockwise.
(2)Left-circularly polarized light: If counterclockwise.


The electric field vector makes one complete revolution as the light advances one wavelength toward you.
The light may be produced by passing linearly polarized light through a quarter-wave plate at an angle of 45° to the PhysicsNTHU optic axis of the plate.
Lab. 22B - Polarization of Light 實驗22B:光的偏振
I. Object (目的) Observe the polarization phenomena of light. 觀察光的偏振現象
PhysicsNTHU MFTai-戴明鳳
Roadmap for Polarization of Light

荧光光谱法英文

荧光光谱法英文

荧光光谱法英文Fluorescence SpectroscopyFluorescence spectroscopy is a powerful analytical technique that has found widespread applications in various fields, including chemistry, biology, materials science, and environmental studies. This analytical method is based on the measurement of the emission of light by a substance that has been excited by the absorption of light or other forms of energy. The process of fluorescence involves the absorption of energy by molecules or atoms, followed by the subsequent emission of light at a longer wavelength than the absorbed light.The fundamental principle of fluorescence spectroscopy is that when a molecule or atom is exposed to light, it can absorb the energy of the incoming photons, causing electrons within the molecule or atom to be excited to higher energy levels. This excitation is a temporary state, and the electrons will eventually return to their ground state, releasing the excess energy in the form of a photon. The energy of the emitted photon is typically lower than the energy of the absorbed photon, resulting in a shift in the wavelength of the emitted light compared to the absorbed light. This wavelength shift is known as the Stokes shift, and it is a key characteristic offluorescence.The intensity and wavelength of the emitted light are influenced by various factors, such as the chemical structure of the fluorescent molecule, the solvent environment, temperature, and the presence of other compounds that can interact with the excited molecules. By analyzing the characteristics of the emitted light, researchers can gain valuable insights into the properties and behavior of the sample under investigation.Fluorescence spectroscopy has a wide range of applications in various fields. In chemistry, it is used for the identification and quantification of organic and inorganic compounds, as well as the study of reaction kinetics and molecular interactions. In biology, fluorescence spectroscopy is employed for the investigation of protein structure and dynamics, the detection and quantification of biomolecules, and the study of cellular processes. In materials science, this technique is used to characterize the properties of polymers, semiconductors, and nanomaterials, among others.One of the key advantages of fluorescence spectroscopy is its high sensitivity, which allows for the detection and quantification of analytes at very low concentrations. Additionally, the technique is non-invasive and can be performed in real-time, making it a valuable tool for in-situ and online monitoring applications. Furthermore, thedevelopment of advanced fluorescent probes and labeling techniques has expanded the versatility of fluorescence spectroscopy, enabling the visualization and tracking of specific molecules or cellular components in complex biological systems.Despite its many benefits, fluorescence spectroscopy also faces some limitations. The presence of interfering compounds, quenching effects, and the potential for photobleaching of the fluorescent molecules can challenge the reliability and accuracy of the measurements. Researchers are constantly working to address these challenges through the development of new instrumentation, data analysis methods, and sample preparation techniques.In conclusion, fluorescence spectroscopy is a powerful and versatile analytical tool that has made significant contributions to various scientific disciplines. As technology continues to advance, the applications of this technique are expected to expand further, providing researchers with new opportunities to gain a deeper understanding of the world around us.。

三维荧光光谱 英语

三维荧光光谱 英语

三维荧光光谱英语Three-Dimensional Fluorescence Spectroscopy: Principles, Applications, and Future Prospects.Fluorescence spectroscopy is a powerful analytical tool that has found widespread applications in various fields ranging from biochemistry to environmental science. Traditionally, fluorescence spectroscopy has been primarily based on two-dimensional (2D) representations, such as excitation-emission matrices (EEMs). However, with the advent of advanced instrumentation and data processing techniques, three-dimensional (3D) fluorescencespectroscopy has emerged as a more comprehensive and informative approach.Principles of Three-Dimensional Fluorescence Spectroscopy.Three-dimensional fluorescence spectroscopy involvesthe measurement of fluorescence intensity as a function ofboth excitation and emission wavelengths, resulting in a three-dimensional dataset. This dataset can be represented as a fluorescence landscape or cube, where each point on the cube corresponds to a specific excitation-emission wavelength pair and the associated fluorescence intensity.The key principle underlying 3D fluorescence spectroscopy is the ability to resolve overlapping fluorescent components present in a complex mixture. This is achieved by analyzing the spectral features of each component in three dimensions, rather than just two. By doing so, 3D fluorescence spectroscopy can provide a more detailed and accurate representation of the fluorescence properties of the sample.Applications of Three-Dimensional Fluorescence Spectroscopy.1. Environmental Science: 3D fluorescence spectroscopy has been widely used in environmental science for the analysis of dissolved organic matter (DOM) in aquatic systems. DOM plays a crucial role in aquatic ecosystems,affecting water quality, biogeochemical cycling, and thefate of contaminants. By employing 3D fluorescence spectroscopy, researchers can characterize the composition and distribution of DOM, gaining insights into its origin, degradation, and ecological implications.2. Biochemistry and Biomedicine: In the field of biochemistry and biomedicine, 3D fluorescence spectroscopy has found applications in the study of protein-ligand interactions, DNA/RNA analysis, and cellular metabolism. By analyzing the fluorescence signatures of biomolecules in three dimensions, researchers can gain a deeper understanding of their structural and functional properties, as well as their interactions with other molecules.3. Food Science and Technology: Three-dimensional fluorescence spectroscopy has also been applied in food science and technology for the quality assessment and authentication of food products. Fluorescence spectroscopy can provide information about the presence andconcentration of various components in food, such as pigments, fats, and proteins. This information can be usedto monitor the freshness, authenticity, and safety of food products.Future Prospects of Three-Dimensional Fluorescence Spectroscopy.With the continuous development of instrumentation and data analysis techniques, the future of 3D fluorescence spectroscopy looks promising. Future research in this field is expected to focus on several areas:1. Advanced Instrumentation: The development of more sensitive and robust instrumentation will further enhance the capabilities of 3D fluorescence spectroscopy. This includes the development of new excitation sources, detectors, and optical components that can improve the signal-to-noise ratio and spectral resolution.2. Advanced Data Analysis Techniques: As the complexity of fluorescence datasets increases, the development of advanced data analysis techniques becomes crucial. Future research will likely focus on the development of algorithmsand methods that can effectively handle large and complex fluorescence datasets, extracting meaningful information and insights.3. Multimodal Analysis: The integration of 3D fluorescence spectroscopy with other spectroscopic and imaging techniques, such as Raman spectroscopy, infrared spectroscopy, and microscopy, will provide a more comprehensive understanding of the sample. This multimodal analysis approach can help researchers gain a deeper understanding of the structure, composition, and dynamics of complex systems.4. Real-Time Monitoring and Automation: The development of real-time monitoring and automation capabilities will enable 3D fluorescence spectroscopy to be used in online and in-process applications. This will help improve the efficiency and accuracy of monitoring and control tasks in various industries, such as environmental monitoring, food processing, and biotechnology.In conclusion, three-dimensional fluorescencespectroscopy has emerged as a powerful analytical tool that offers unique insights into the fluorescence properties of complex systems. With the continuous development of instrumentation and data analysis techniques, its applications in various fields are expected to expand further, leading to new scientific discoveries and technological advancements.。

可见光分光光度法,英文

可见光分光光度法,英文
2. Determine the absorption maximum: Identify the wavelength at which the absorption is highest on the absorption curve
Analysis of experimental results of visible light spectroscopy
目录
Introduction to visible light spectroscopy
Definition
Visible light spectrophotometry is a quantitative and qualitative analysis method based on the characteristics of substances absorbing visible light in the wavelength range (400-760 nm).
Visible light spectrophotometry
Introduction to visible light spectroscopyExperimental technique of visible light spectroscopyAnalysis of experimental results of visible light spectroscopyPrecautions and safety in visible light spectrum experiments
01
Wear safety goggles, gloves, and lab coat to protect against splashes and spills

温度响应型酰腙可逆共价键水凝胶的制备及性能

温度响应型酰腙可逆共价键水凝胶的制备及性能

Vol. 35 No. 1功 能 高 分 子 学 报2022 年 2 月Journal of Functional Polymers93文章编号: 1008-9357(2022)01-0093-08DOI: 10.14133/ki.1008-9357.20210322002温度响应型酰腙可逆共价键水凝胶的制备及性能何 元1, 罗媛媛2, 刘 通1, 张银山1, 郭赞如1, 章家立1(1. 华东交通大学材料科学与工程学院,高分子材料与工程系,南昌 330013;2. 重庆市计量质量检测研究院,重庆 401120)摘 要: 首先,通过可逆加成-断裂转移(RAFT)聚合制备了丙烯酰胺(AM)、双丙酮丙烯酰胺(DAAM)和N-异丙基丙烯酰胺(NIPAM)的共聚物(PAM-co-PDAAM-co-PNIPAM);然后,使PAM-co-PDAAM-co-PNIPAM与己二酸二酰肼(ADH)反应后,得到了具有温度和pH双重响应性的水凝胶。

通过核磁共振氢谱(1H-NMR)和凝胶渗透色谱(GPC)、流变仪、扫描电镜(SEM)以及傅里叶变换红外光谱(FT-IR)对共聚物和水凝胶的结构和组成,以及水凝胶的温度和pH双重响应行为进行了研究。

研究表明,该水凝胶具有温度调控的自愈合性,对药物阿霉素(Dox)表现出pH和温度双重响应的可控释放行为。

关键词: 智能水凝胶;酰腙可逆共价键;自愈合;温度响应中图分类号: O633 文献标志码: APreparation and Properties of Temperature-Responsive HydrogelsBased on Acylhydrazone Reversible Covalent BondsAll Rights Reserved.HE Yuan1, LUO Yuanyuan2, LIU Tong1, ZHANG Yinshan1, GUO Zanru1, ZHANG Jiali1(1. Department of Polymer Materials and Engineering, School of Materials Science and Engineering, East China JiaotongUniversity, Nanchang 330013, China; 2. Chongqing Academy of Metrology andQuality Inspection, Chongqing 401120, China)Abstract: A series of PAM-co-PDAAM-co-PNIPAM copolymers were synthesized by reversible addition fracture transfer(RAFT) polymerization from acrylamide (AM), diacetone acrylamide (DAAM) and N-isopropylacrylamide (NIPAM). Theirstructure and composition were characterized by Nuclear Magnetic Resonance (NMR) and Gel Permeation Chromatography(GPC). Hydrogel with pH and temperature dual-response formed by the acyl hydrazone dynamic bonds between ketocarbonylin polymer and hydrazide in adipic dihydrazide (ADH). The dual-responsive behavior of hydrogels to temperature and pHwas researched by rheological measurement, Scanning Electron Microscope (SEM) and Fourier Transform Infrared (FT-IR)spectroscopy. At the same time, the hydrogel demonstrated temperature controlled self-healing properties. Besides, thehydrogels showed pH-and temperature-responsive controlled release behaviors for doxorubicin(Dox).Key words: smart gel; acylhydrazone dynamic covalent bond; self-healing; temperature response收稿日期: 2021-03-22基金项目: 国家自然科学基金(21802041,51563009,21865009);江西省杰出青年基金(20202ACBL214001)作者简介: 何 元(1994—),男,硕士,主要研究方向为功能高分子材料。

POLAR塑料闪烁体的非线性能量响应研究

POLAR塑料闪烁体的非线性能量响应研究

1.Introduction 2. Method 3. Data reduction 4. Discussion
1.1 Introduction to POLAR 1.2 Motivation
. Introduction to POLAR 线 :极端高能;复杂光变;复杂能谱;爆发机制尚不清
楚;天体物理领域的前沿与热点; 科学 的偏振; 探测 理:Compton 散射中,散射光子的角分布在垂直于入射光 :探测伽玛射线暴(GRB)释放出的硬 X 射线/软 γ 射线
.
POLAR 塑料闪烁体的非线性能量响应研究
1.Introduction 2. Method 3. Data reduction 4. Discussion
1.1 Introduction to POLAR 1.2 Motivation
. Introduction to POLAR
科学 的偏振;
:探测伽玛射线暴(GRB)释放出的硬 X 射线/软 γ 射线
1.Introduction 2. Method 3. Data reduction 4. Discussion
1.1 Introduction to POLAR 1.2 Motivation
. Motivation
电子在塑料闪烁体(PS)内沉积能量,PS 的分子被电离或 激发,并在退激发过程中释放出荧光。 的 能 的 系 来描述: 光 与电子 非线性的,可由半经验性的 Birks’ law
1.Introduction 2. Method 3. Data reduction 4. Discussion
1.1 Introduction to POLAR 1.2 Motivation
. Introduction to POLAR 线 :极端高能;复杂光变;复杂能谱;爆发机制尚不清

光学设计常用术语解释及英 汉对照翻译汇总

光学设计常用术语解释及英    汉对照翻译汇总

第一部分最基本的术语及英汉对照翻译1、时谱:time-spectrumIn this paper, the time-spectrum characteristics of temporal coherence on the double-modes He-Ne laser have been analyzed and studied mainly from the theory, and relative time-spectrum formulas and experimental results have been given. Finally, this article still discusses the possible application of TC time-spectrum on the double-mode He-Ne Iaser.本文重点从理论上分析研究了双纵模He-Ne激光时间相干度的时谱特性(以下简称TC时谱特性),给出了相应的时谱公式与实验结果,并就双纵模He-Ne激光TC时谱特性的可能应用进行了初步的理论探讨。

2、光谱:SpectraStudy on the Applications of Resonance Rayleigh Scattering Spectra in Natural Medicine Analysis共振瑞利散射光谱在天然药物分析中的应用研究3、光谱仪:spectrometerStudy on Signal Processing and Analysing System of Micro Spectrometer 微型光谱仪信号处理与分析系统的研究4、单帧:single frameComposition method of color stereo image based on single fram e image 基于单帧图像的彩色立体图像的生成5、探测系统:Detection SystemResearch on Image Restoration Algorithms in Imaging Detection System 成像探测系统图像复原算法研究6、超光谱:Hyper-SpectralResearch on Key Technology of Hyper-Spectral Remote Sensing Image Processing超光谱遥感图像处理关键技术研究7、多光谱:multispectral multi-spectral multi-spectrumSimple Method to Compose Multi spectral Remote Sensing Data Using BMP Image File用 BMP 图像文件合成多光谱遥感图像的简单方法8、色散:dispersionResearches on Adaptive Technology of Compensation for Polarization Mode Dispersion偏振模色散动态补偿技术研究9、球差:spherical aberrationThe influence of thermal effects in a beam control system and spherical aberration on the laser beam quality光束控制系统热效应与球差对激光光束质量的影响10、慧差:comaThe maximum sensitivity of coma aberration evaluation is aboutλ/25;估值波面慧差的极限灵敏度为λ/25;11、焦距:focal distanceAbsolute errors of the measured output focal distance range from –120 to 120μm.利用轴向扫描法确定透镜出口焦距时的绝对误差在–120—120μm之间。

ROTI计算策略的初步分析比较

ROTI计算策略的初步分析比较

ROTI计算策略的初步分析比较邵冷冷;宋淑丽【摘要】由电离层闪烁和TEC(Total Electron Content)监测仪获取的振幅闪烁指数S 4和相位闪烁指数σ?是电离层闪烁研究中最常用的参数,由双频GNSS(Global Naviga-tion Satellite System)接收机获取的电离层TEC变化率指数ROTI(Rate of TEC In-dex)与S 4指数的相关性已得到很多相关研究的验证,ROTI也是电离层闪烁研究的一个有效参数,这样就使利用全球分布的大量GNSS观测数据开展电离层闪烁研究成为可能.但是在不同的研究中计算ROTI所使用数据的采样率和计算间隔有所差异,对于计算策略的选择尚无定论.利用海南三亚1 s、15 s和30 s采样率的GNSS双频观测数据与电离层闪烁和TEC监测仪获取的S 4指数,分析了在电离层闪烁发生时,不同计算策略获取的各类ROTI与S 4指数的相关性,分析比对了几类ROTI对电离层闪烁的敏感性.分析结果表明:各类ROTI与S 4指数都具有较强的相关性,在大多数情况下,不同种类ROTI都可以在闪烁发生期间响应S 4指数的变化;不同采样率的ROTI在响应S 4指数变化时,判断是否发生电离层闪烁事件的阈值有所差异;由于ROTI和S 4指数监测电离层闪烁的机理不同,也会出现几个参数不能同时反映电离层受扰动的情况,在进行电离层闪烁监测、预报和预警时,建议同时采用多个参数综合分析;在同等的电离层条件下,15 s和30 s采样率的ROTI 在数值上比较接近,但是两者明显小于1 s采样率的ROTI.使用GNSS接收机进行电离层闪烁观测时,建议采用高于1 s采样率的GNSS观测数据.%The amplitude scintillation index S 4 and the phase scintillation indexσ?acquired by the ionospheric scintillation and TEC (Total Electron Content) monitor are the most commonly used indices for the ionospheric scintillation study. The correlation between the change rate of TEC index ROTI, which is obtainedfrom the dual frequen-cy GNSS (Global Navigation Satellite System) receiver, and S 4 index is verified. Most of the researches show that ROTI is sufficient for ionospheric scintillation studies. It is possible to conduct ionospheric scintillation study using a large number of globally distributed GNSS observations. However, the data sampling rate and ROTI calculation interval used in respective studies are different, and the choice of calculation strategy is inconclusive. GNSS dual-frequency observation data of 1 s, 15 s, and 30 s sampling rates and S 4 index obtained by ionospheric scintillation and TEC monitor are used to analyze the correlation between ROTI and S4 index which are obtained from different calculation strategies during the ionospheric scintillation in Sanya, Hainan province. Besides, the sensitivity of the difference between ROTI and ionospheric scintillation is analyzed and compared. The result shows that different ROTIs have a strong correla-tion with S4 index. The different kinds of ROTI can respond to S4 index during the ionospheric scintillation. The different sampling ROTI have different threshold values to determine whether the scintillation occurs responding to S4 index changes. Due to the different mechanisms of ROTI and S 4 index monitoring ionospheric scintillation, they will not reflect the ionospheric disturbance at the same time in some cases. It is suggested that multiple parameters should be analyzed for ionospheric scintillation monitoring, forecast, and forewarning. Under the same ionospheric scintillation con-dition, the ROTI values of 15 s and 30 s sampling are relatively close, but they are significantly lower than the ROTI values of 1 s sampling rate. It is recommended to select the sampling rateof GNSS observations more than 1 s to monitor ionospheric scintillation using GNSS receivers.【期刊名称】《天文学报》【年(卷),期】2017(058)006【总页数】13页(P88-100)【关键词】行星和卫星:大气,S4指数,;ROTI,电离层闪烁,方法:数据分析【作者】邵冷冷;宋淑丽【作者单位】中国科学院上海天文台上海200030;中国科学院大学北京100049;中国科学院上海天文台上海200030【正文语种】中文【中图分类】P1261 引言电离层E层和F层电子密度的不均匀性,会导致穿过电离层的无线电信号发生振幅和相位的快速随机起伏,这种现象称为电离层闪烁[1].GNSS(Global Navigation Satellite System)发射的无线电信标载波频率在L波段范围内,非常容易受电离层闪烁活动的影响[2],其影响主要包括以下两个方面:首先,电离层闪烁对GNSS信号的影响不能通过电离层延迟的双频组合技术消除,强烈的信号抖动会造成接收机跟踪GNSS卫星失败,即卫星信号失锁,这会增加导航定位误差,严重时甚至会导致导航定位失败[3];其次,电离层闪烁中的不规则结构体会影响电离层TEC(Total Electron Content)的准确模型化,影响电离层模型的精度,从而影响导航系统的服务性能[4].振幅闪烁指数S4和相位闪烁指数σϕ是电离层闪烁研究中最常用的参数,但是它们需要利用GNSS接收机改进后的电离层闪烁和TEC监测仪来获得,并且该设备大范围布设的应用、技术价值不高,因此不利于大范围的电离层闪烁监测.根据双频GNSS接收机观测可以准确获得传播路径上电离层TEC变化率ROT(Rate of TEC)的思想,Pi等[5]提出了利用TEC变化率指数ROTI(Rate of TEC Index)监测引起电离层闪烁的不规则结构体的方法.Basu等[6]的研究结果表明:ROTI和S4指数存在一定的相关性,都可以用来监测引起电离层闪烁的不规则结构体,两者在监测不规则结构体生成、演化和消散的过程中有着相同的趋势[7].Carrano等[8]利用10 Hz采样率的GPS观测数据获得的1 min计算间隔的ROTI 监测到了低于菲涅耳尺度的不规则结构体;熊波等[9]利用15 s采样率的GPS观测数据获得的1 min计算间隔的ROTI对电离层闪烁进行了监测,并总结了ROTI响应S4指数闪烁事件的准确率;Tanna等[10]基于30 s采样率的GPS观测数据5 min计算间隔的ROTI,研究了在低纬度带不同经度上磁暴对不规则结构体的生成和抑制作用;Jacobsen等[11-12]利用1 s采样率的GPS观测数据和5 min计算间隔的ROTI监测了高纬度地区的电离层扰动,并利用1 s和30 s采样率的GPS观测数据,分析了不同采样率和不同计算间隔计算出的ROTI间的相关性,得出不同采样率相同计算间隔的ROTI呈正相关的结论.Yang等[13]对香港地区的电离层闪烁利用1 s采样率的GPS观测数据和5 min计算间隔的ROTI分析了ROTI与S4指数的比率关系.综上所述,在ROTI和S4指数的相关性、ROTI监测电离层扰动等研究中,不同的研究采用了不同的计算策略,但是目前还缺少对不同计算策略得到的各类ROTI与S4指数的综合比较分析.我国大部分低纬度地区处于赤道异常峰的北峰区域,如:广东、广西、海南等地区是全球范围内电离层闪烁发生最频繁、受影响最严重的地区之一[14].中国大陆构造环境监测网络(Crustal Movement Observation Network of China,CMONOC)站点分布广泛,为电离层变化率指数ROTI的研究提供了丰富的数据,为大区域的电离层ROTI监测和研究提供了条件.本文利用CMONOC海南三亚站(18.3°N,109.6°E)提供的GNSS观测数据和位于海南三亚的电离层闪烁与TEC监测仪获得的电离层闪烁实测数据,综合研究了不同采样率和计算间隔的ROTI与S4指数的相关性、各类ROTI与S4指数监测电离层闪烁时表现出来的不同特征,为利用ROTI研究电离层闪烁计算策略的选择和应用提供参考.2 计算方法S4指数是衡量电离层闪烁强度的重要指标,可由GNSS信号强度计算得到,通常每分钟计算得到一个值,定义为信号强度平均值归一化的标准差[15],计算公式如下:式中SI表示信号强度,角括号〈〉表示60 s间隔内全部观测值的算术平均值.相位抖动通常也作为电离层闪烁研究的主要参数.在相位闪烁监测中,相位闪烁指数σϕ定义为消趋势后的L1载波相位1 min内的标准差[16]:其中,ϕ为消趋势后的载波相位,单位为rad,σϕ反映了卫星信号相位变化的剧烈程度.ROT用来描述在倾斜路径上电离层TEC的变化率,单位为TECU/min.ROTI定义为在一定时间间隔内ROT的标准差,单位为TECU/min.算法如下[12]:式中,LGF(i)是i时刻GNSS双频观测的几何无关线性组合方程,L1和L2为相位观测值, λ1和λ2为对应载波频率的波长,Δt是相邻两个历元间的时间差,单位为min.f1和f2为对应相位观测值的频率,N为参与计算的历元数.本文利用1 s、15 s 和30 s采样率的GNSS观测数据,获得了1 min和5 min计算间隔不同种类的ROTI,具体的计算策略如表1所示.表1 计算各类ROTI的参数设定Table 1_Selected parameters for calculating various types of ROTI_Sampling/s___Time interval of ROTIcalculation/min____________ Number 1 60 1 5 300 1 15 1 4 15 5 20_____30___________________________________________________ _ 5_10ROTI的计算使用滑动窗口法,滑动时把窗口内的中间历元时刻当作该ROTI的时刻,滑动窗口的长度以时间为单位,如1 min或5 min.为了确保统计结果的有效性,进行数据处理时,在该时间区间内,只有ROT个数大于设定值时,才解算当前的ROTI,如30 s采样率和5 min计算间隔的ROTI需要在该窗口内有8个以上的ROT,利用1 s采样率和1 min计算间隔的ROTI,需要在该窗口内有50个以上的ROT,否则继续往下滑动,直到完成所有ROTI的计算.在计算ROTI之前,要对RINEX(Receiver Independent Exchange)观测文件中的相位观测值进行预处理.首先,使用Melbourne-Wbbena(M-W)组合和电离层(LG)组合进行周跳探测与粗差剔除[17];其次,为了消除多路径噪声对计算结果的影响,去除了高度角在30°以下的GNSS观测数据.3 数据分析3.1 闪烁事件本文所采用的振幅闪烁指数S4和相位闪烁指数σϕ由位于海南三亚的电离层闪烁与TEC监测仪实测获得,S4指数和σϕ均为每分钟一个值.根据三亚电离层闪烁与TEC监测仪的工作情况,本文选取了海南三亚2015年4月份的电离层闪烁观测数据进行了统计分析.图1为海南三亚2015年4月7日到23日的S4指数、σϕ的时间序列,因接收机断电等原因未能连续观测造成部分数据缺失.从图1中S4指数的时间序列可以看出:在4月7日、8日、13日、15日、22日、23日当地均发生了显著的电离层闪烁事件,S4指数最大值均超过了0.5.在闪烁事件的统计中,S4指数闪烁主要为0.3<S4<0.5级别的闪烁,0.2<S4<0.3和S4>0.5的闪烁事件则相对较少.在S4指数发生闪烁的情况下,σϕ也有一定的响应,但是相对于S4指数的变化,σϕ的变化则相对较弱.在振幅闪烁研究中,通常以S4> 0.2作为判断电离层闪烁事件发生的条件[18];在相位闪烁的研究中,当σϕ> 0.1时,则认为发生了电离层闪烁事件[19].通过比较图2中4月22日G02和G04 σϕ随S4指数的变化可知:当振幅闪烁指数S4发生闪烁时,相位闪烁指数σϕ则可能达不到闪烁的量级,如G02在17:30前后的一段时间内就出现了此类情况.因此,在随后的研究中,选择S4指数作为判断是否发生电离层闪烁事件的标准.3.2 各类ROTI闪烁阈值的确定国内外许多学者对利用ROTI判断不规则结构体的出现作了大量的研究.Ma等[20]的研究中把ROTI>0.5 TECU/min作为出现不规则结构体的判断条件,Zou等[18]的研究中也说明了当ROTI>0.5 TECU/min时则表示出现了数千米尺度的不规则结构体;黄丽等[21]在研究SED(Storm Enhanced Density,SED)边界附近电离层不规则结构体时,以ROTI>1 TECU/min来判断是否存在小尺度的不规则结构体.本文利用1 s、15 s和30 s不同采样率的GNSS观测数据和不同计算间隔计算的ROTI,分析闪烁事件发生时不同种类ROTI能否很好地响应S4指数的变化.图3统计了2015年4月18日G04卫星不同采样率ROT的变化,从图中可以看出,在未发生闪烁时,30 s和15 s采样率ROT的标准差(STD)分别为0.355 TECU/min和0.357 TECU/min,1 s采样率ROT的标准差为0.57 TECU/min.在发生闪烁时,由图4中2015年4月13日G10不同采样率ROT的变化可知:30 s和15 s采样率ROT的标准差分别为0.955 TECU/min和1.07 TECU/min.1 s采样率ROT的标准差为2.18 TECU/min.由此可知,在相同的电离层条件下,ROT的振幅变化随采样率的增大而增大;对于同一种采样率的数据,在发生电离层闪烁期间, ROT的振幅会明显变大.图1 2015年4月7日到23日S4指数和σϕ的时间序列Fig.1 Time series of S4 index and σϕfrom April 7 to 23 in 2015图2 2015年4月22日G02卫星和G04卫星的S4指数和σϕ的时间序列Fig.2 Tim e series of S4 index and σϕof PRN G02 and PRN G04 on 2015 April 22 图3 2015年4月18日G04无电离层闪烁时各参数时间序列Fig.3 Time series of different parameters of G04 without ionospheric scintillation on 2015 April 18图4 2015年4月13日G10闪烁条件下各参数时间序列Fig.4 Time series of different parameters of G10 with ionospheric scintillation on 2015 April 13 在平静电离层条件下,不同计算策略的各类ROTI的最大值是不同的.30 s-5 min ROTI、15 s-1 min ROTI和15 s-5 min ROTI的最大值(MAX)约为0.1TECU/min,而1 s-1 min ROTI和1 s-5 min ROTI的最大值均超过了0.5 TECU/min.因此,对于不同采样率的GNSS观测数据,在利用ROTI响应S4指数的变化时,则需要采取不同的计算策略.此外,根据不同的GPS卫星统计了电离层闪烁事件.在所选取的观测时段内,GPS卫星共出现了60次振幅闪烁事件.在发生振幅闪烁和未发生振幅闪烁时各类ROTI最大值大于一定阈值的统计结果如表2所示.在30 s-5 min ROTI计算策略下,发生振幅闪烁时ROTI在0.5 TECU/min以上所占的百分比为95%,在未发生振幅闪烁时ROTI在0.5 TECU/min以上所占的百分比为1.96%.因此,在未发生闪烁的统计中,对于15 s-1 min和15 s-5 min的ROTI阈值设在0.5 TECU/min以上即可,对于1 s-1 min ROTI和1 s-5 min ROTI阈值则需要设在1.4 TECU/min和1.2 TECU/min以上;在发生闪烁的统计中,15 s-1 min ROTI对应的阈值为0.7 TECU/min,15 s-5 min ROTI对应的阈值为0.6TECU/min,而1 s-1 min ROTI和1 s-5 min ROTI对应的阈值则都为1.5 TECU/min.因此,在下面的分析中,对于5 min计算间隔的ROTI,以30 s采样率数据计算的ROTI>0.5 TECU/min、15 s采样率数据计算的ROTI>0.6 TECU/min和1 s采样率数据计算的ROTI>1.5 TECU/min作为发生电离层闪烁的判断条件.通过图4电离层闪烁条件下不同采样率相同计算间隔的各类ROTI的比较可以看出:计算间隔相同时,采样率越高,则ROTI的峰值越大.表2 闪烁和非闪烁时各类ROTI最大值大于阈值的统计Table 2 Statistics of various ROTI maximums greater than their threshold values under the circumstances of ionospheric scintillation and non-scintillation_Sampling-Interval____ROTI threshold____Scintillation/%____Non-scint_________ illation/% 30 s-5 min 0.5 95.00 1.96 15 s-1 min 0.5 98.33 1.96 15 s-1 min 0.6 96.670.98 15 s-1 min 0.7 95.00 0.73 15 s-5 min 0.5 96.67 0.98 15 s-5 min 0.693.33 0.49 15 s-5 min 0.7 91.67 0.24 1 s-1 mim 1.1 98.33 8.07 1 s-1 mim 1.2 96.67 4.4 1 s-1 mim 1.3 96.67 2.44 1 s-1 mim 1.4 96.67 1.22 1 s-1 mim 1.595.00 0.73 1 s-5 mim 1.1 96.67 2.2 1 s-5 mim 1.2 96.67 0.49 1 s-5 mim 1.396.67 0.49 1 s-5 mim 1.4 96.67 0.49 _____1 s-5mim_________________________________________________________ _ 1.5_95.00_0.24 3.3 各类ROTI与S4指数的相关性分析在一般情况下,ROTI可以较好地响应S4指数的变化,当S4指数增大时,ROTI也随之增大,当S4指数变小或处于平静时,ROTI也随之减小或进入平静状态.图5给出了2015年4月13日到4月19日S4指数与不同种类ROTI的时间序列,从图中可以看出, 4月13日发生了一次强闪烁事件,闪烁的强度大、持续时间长、受影响的卫星数目多,各类ROTI响应都比较强烈,总体上和各颗卫星的S4指数变化一致;在4月15日,发生了较小的电离层闪烁事件,闪烁的强度弱、持续时间短、受影响的卫星数少,但是总体上各类ROTI同样很好地响应了S4指数的变化.由此可以得出,不同种类ROTI都可以很好地响应S4指数的变化,均可以作为电离层闪烁研究的参数.图5 2015年4月13日到19日S4指数与各类ROTI的时间序列Fig.5 Time series of S4 index and different ROTI from April 13 to 19 in 2015从图5统计的海南三亚2015年4月13日到19日不同种类ROTI的时间序列可以看出: 13日、14日和15日各类ROTI的量级均达到了电离层闪烁事件的级别,而且各类ROTI都能很好地响应S4指数闪烁事件.从各类ROTI与S4指数变化趋势的一致性可以得出,两者有一定的相关性.图6(a)中统计了观测时段内S4>0.2的闪烁事件与各类ROTI的相关系数(Corr.Coef),可知:各类ROTI与S4指数在整体上都是强相关的,并且相关系数差距不大.此外,从各类ROTI与S4指数的比率关系来看,对于30 s-5 min和15 s-1 min计算策略的ROTI,大致存在ROTI=3S4的关系,对15 s-5 min计算策略的ROTI则大致有ROTI=4S4的关系,而1 s-1 min和1 s-5 min计算策略则约为ROTI=9S4的关系.图6(b)—(f)统计了2015年4月15日发生闪烁事件时各类ROTI与S4指数的相关系数,直线的斜率表示ROTI与S4指数的比率关系.可以看出:对于不同的闪烁条件,S4指数与各类ROTI的相关系数可能会有所不同,但是在总体上1 s采样率的GNSS观测数据计算得到的ROTI的相关系数略高于30 s和15 s采样率的ROTI,在ROTI和S4指数整体的比率关系上也可以得出相似的结论.图6 ROTI与S4指数的相关性.(a)为2015年4月闪烁发生时各类ROTI与S4指数的相关系数及比率关系,(b)、(c)、(d)、(e)和(f)为2015年4月15日发生闪烁事件时S4指数和各类ROTI的相关系数及比率关系.Fig.6 The correlation between ROTI and S4 index.(a)is the correlation coeffcient and ratio between ROTI and S4 index during ionospheric scintillation in April2015.(b),(c),(d),(e),and(f)are thecorrelation coeffcient and ratio between different ROTI and S4 index on 2015 April 15.3.4 各类ROTI与S4指数的特征分析振幅闪烁的形成与不规则结构体的第一菲涅耳尺度有关,满足R为不规则结构体的大小,λ为信号的波长,z为不规则结构体的平均高度[22].对于GPS L1载波,λ=0.1903 m,假设不规则结构体的平均高度为z=350 km,则引起闪烁的不规则结构体的尺度约为365 m.ROTI由卫星穿刺点的运动速度与不规则结构体在卫星信号传播路径的垂直方向上的漂移速度矢量和以及Nyquist周期决定[23].对于30 s 采样率的ROTI, Nyquist周期为60 s,如果当卫星星下点轨迹的运动速度和不规则结构体正交于传播路径的漂移速度矢量和大致为100 m/s时,那么ROTI对应的不规则结构的空间尺度大约为6 km.由于ROTI和S4指数的监测机制存在差异,因此同时利用ROTI和S4指数监测电离层闪烁时也会出现一些差异.各类ROTI和S4指数在大多数情况下,都可以同时反映电离层扰动的变化,但是在一些情况下也会表现出不同的特征.在图7(a)中,S4指数闪烁事件发生的时间很短暂,各类ROTI都得到了即时响应,这进一步验证了不同种类ROTI监测电离层闪烁的可靠性.在图7(b)中出现了在S4指数闪烁结束后,ROTI闪烁还会持续的现象.图7(c)中则出现了S4指数发生闪烁,而各类ROTI均未出现闪烁的情况.在图7(d)中当卫星仰角接近30°时,从S4指数看,并没有发生闪烁事件,但是各类ROTI都达到了闪烁的级别.根据ROTI和S4指数监测电离层闪烁的不同机制,可以选择不同的参数来实现不同的目的.S4指数可以监测第一菲涅耳尺度大小的不规则结构体,并且可以更为直观、准确地表现出电离层闪烁发生的剧烈程度;ROTI和S4指数存在一个比率关系,对于同一个S4指数闪烁事件,由于不规则结构体的漂移速度不相同等原因,ROTI的大小也可能会不同.对于不同采样率的GNSS观测数据,对于较低的采样率,如30 s和15 s采样率的GNSS观测数据,可以节省更多的存储空间,数据处理速度相对较快,缺点是难以监测造成GNSS信号闪烁的第一菲涅耳尺度大小的不规则结构体;对于较高的采样率,例如1 s采样率的GNSS观测数据,相同时段的数据需要占用更多的存储空间,数据处理的速度也相对较慢,但有可以监测到小于第一菲涅耳尺度的不规则结构体的优势.因此,基于GNSS的S4指数、不同种类的ROTI进行业务类的电离层闪烁的监测、预报和预警时,需要进行更为深入的研究.4 讨论与小结电离层闪烁事件常发生于低纬赤道区和高纬度地区[24],是一种常见的电离层空间天气现象.目前电离层闪烁的监测有多种手段,重要的手段之一是GNSS电离层闪烁和TEC监测仪获得的振幅闪烁指数S4和相位闪烁指数σϕ.电离层闪烁接收机主要安装在电离层闪烁发生频繁的区域,如低纬赤道区和极区.GNSS双频接收机为开展更大范围的电离层闪烁事件的监测提供了机会.电离层变化率指数ROTI能较好地反映电离层扰动条件下各卫星的信号发生异变的情况,也是电离层闪烁研究的一个常用参数.由于ROTI监测的可实现性强,在不具备电离层闪烁和TEC监测仪的区域,可以通过双频GNSS接收机获取的观测数据计算出的ROTI对电离层闪烁事件进行监测.图7 S4指数和各类ROTI在监测电离层闪烁时的不同表现.(a)2015年4月14日G07卫星,(b)2015年4月7日G20卫星, (c)2015年4月7日G02卫星,(d)2015年4月21日G07卫星Fig.7 The different performances of S4 index and different ROTI in monitoring ionospheric scintillation.(a)2015 April 14 G07 satellite,(b)2015 April 7 G20 satellite,(c)2015 April 7 G02satellite,and(d)2015 April 21 G07 satellite本文的分析基于S4指数的不同采样率和不同计算间隔计算得到的ROTI,得出如下初步结论:(1)在通常情况下,不同种类的ROTI都可以很好地响应S4指数的变化,强相关且相关系数差异不大;(2)不同采样率的ROTI在响应S4指数变化时判别是否发生闪烁的阈值不同.数据分析结果表明:对于5 min计算间隔的ROTI,以30 s采样率的ROTI>0.5 TECU/min、15 s采样率的ROTI>0.6 TECU/min、1 s采样率的ROTI>1.5 TECU/min作为判断是否发生电离层闪烁事件的阈值,相对而言比较合适;(3)ROTI与S4指数存在类似线性的关系,一般采样率越高,则这个比率也越大;并且对于同一采样率的数据,1 min和5 min计算间隔的ROTI与S4指数有较为相似的线性关系;(4)由于ROTI和S4指数监测不规则结构体的机制不同,因此各类ROTI和S4指数在某些情况下会表现出不同的特征;(5)尽管在利用ROTI监测电离层不规则结构体时,可以根据不同的需求和目的选择数据采样率,通常情况下需要采用高于1 s采样率的GNSS观测数据来监测更小尺度的不规则结构体.参考文献[1]Yeh K C,Liu C H.Proceedings of the IEEE,1982,70:324[2]Groves K M,Basu S,Webber E J,et al.RaSc,1997,32:2047[3]Dubey S,Wahi R,Gwal A K.AdSpR,2006,38:2478[4]刘钝,甄卫民.电波科学学报,2012,27:95[5]Pi X,Mannucci A J,Lindqwister U J,et al.GeRL,1997,24:2283[6]Basu S,Groves K M,Quinn J M,et al.JASTP,1999,61:1219[7]Cherniak I,Zakharenkova I,Krankowski A.EP&S,2014,66:1[8]Carrano C S,Groves K.TEC Gradients and Fluctuations at Low Latitudes Measured with High Data Rate GPS Receivers.ION 63rd AnnualMeeting,Cambridge,April 23-25,2007[9]熊波,万卫星,宁百齐,等.地球物理学报,2007,50:1639[10]Tanna H J,Pathak K N.Ap&SS,2014,235:373[11]Jacobsen K S,Dahnn M.JSWSC,2014,4:A27[12]Jacobsen K S.JSWSC,2014,4:A33[13]Yang Z,Liu Z Z.GPS Solutions,2016,20:815[14]黄林峰,邓博昌,黄江,等.空间科学学报,2012,32:25[15]Coco D.GPSW,1991,2:47[16]Beach T L.RaSc,2006,41:1[17]谭争光,郭金运,宗干,等.全球定位系统,2013,38:60[18]Zou Y H,Wang D L.JASTP,2009,71:1948[19]Jiao Y,Morton Y T,Taylor S,et al.RaSc,2013,48:698[20]Ma G,Maruyama T.GeRL,2006,33:1[21]黄丽,袁志刚,邓晓华,等.空间科学学报,2009,29:552[22]Shagimuratov I I,Krankowski A,Ephishov I,et al.EP&S,2012,64:521[23]Tanna H J,Karia S P,Pathak K N.AdSpR,2013,52:412[24]Crane R K.Proceedings of the IEEE,1977,65:180。

  1. 1、下载文档前请自行甄别文档内容的完整性,平台不提供额外的编辑、内容补充、找答案等附加服务。
  2. 2、"仅部分预览"的文档,不可在线预览部分如存在完整性等问题,可反馈申请退款(可完整预览的文档不适用该条件!)。
  3. 3、如文档侵犯您的权益,请联系客服反馈,我们会尽快为您处理(人工客服工作时间:9:00-18:30)。

Research on Light Polarization FSO-OFDM System Zhao Li1 Lei Zhi-yong1 Ren An-hu1Ke Xi-zheng2 Wu Ying-hui11. School of Electronic Information Engineering, Xi’an Technological University, Xi’an China, 7100322. Automation & Information School, Xi’an University of Technology, Xi’an China,710048e-mail: pilly_lily@Abstract—we analyzed the polarization characteristic and the influence of lithometeors on polarized light. We have proved out that it is doable to utilize the polarized light in FSO (Free Space Optical Communication) system. The scheme of optical polarization FSO-OFDM is advanced aimed at the format of the OFDM signals, and the principles of the transceivers are expatiated. At last, this scheme is confirmed by Monte Carlo approach, which shows that performance of the light polarization FSO-OFDM system is very stable.Keywords-Optical Communication; Free Space Optical Communication (FSO); Orthogonal Frequency Division Multiplexing (OFDM); Polarization ModulationI.F OREWORDWith the increasing development of semiconductor laser and photodetector, as well as the improvement in the optical communications technology, free space optical communication technology (FSO) has been widely used. Due to it has combined the superiority of fiber communication, microwave communication and other traditional wireless communication technologies, and it has the advantages of low cost, flexible networking, convenient installation and dispense with frequency permission, therebyit has became an emerging broadband wireless access and has been widely concerned. Although optical communicationis a kind of ultra-wideband technology, the bandwidth can be achieved to 1THz, transfer rate is often limited to 10Gb/s or less due to light dispersion and speed restriction of electronic devices [1]. Orthogonal Frequency Division Multiplexing (OFDM) is a multi-carrier modulation (MCM) technology, whose basic idea is to break down high-speed data stream into multiple orthogonal subcarriers to transmit, so that the symbol rate of the subcarriers dramatic declined, it is particularly suited high-speed wireless data transmission. The technology has been applied to atmospheric laser communications, and acquired some research results[2-5]. Yan Tang, etc defined two types of FSO-OFDM model [6], namely, the direct up/down-conversion model and the intermediate frequency (IF) model. Compared with the IF model, the direct up/down-conversion model does not need optical band-pass filter in the transmitter and receiver, but the anti-interference is lower than the IF mode. The method, which can achieve direct up/down-conversion model by employing the optical polarization properties, is proposed in this paper. As the horizontal polarized light and vertical polarized light pass through the same optical path when using light polarization modulation, and the atmospheric attenuation will be counteracted by each other in the solution of optical polarization angle, hence the FSO-OFDM polarization modulation can reduce or even eliminate the influence of atmospheric channel, and improve the system reliability, and at the same time it can also improve the system confidentiality.II.THE POLARIZED LIGHT PROPERTIES As we all know that only transverse wave has the phenomenon of polarization and longitudinal wave without that phenomenon. Light is a kind of transverse wave, whose vibration direction and propagation direction are vertical for each other, so the plane which is consisted of light’s propagation direction and vibration direction is polarizationFigure 1. The sketch map of the optical propagation Any components, which can produce linearly polarized light, can be referred to as polarization devices. It can be used to obtain or check the linearly polarized light. The polarizing disk has a special optical structure direction which is known as the vibration direction or the polarization direction. The process of the light polarizing and light analyzer are shown in Fig.2 and Fig.3, it can be seen that only the light which is parallel with vibration direction can permeate the polarizing disk. According to the Malus’ law, if the angle between the polarizing disk and polarization analyzer isα, the relation of the incident intensity and the emergent intensity can be written as:2/0SII=(1)αα22cos2cos×=×=SIII (2)IsFigure 2. Light polarizing process2010 International Conference on Electrical and Control EngineeringIFigure 3. Light analyzer processOptical vector satisfies the vector’s decomposition principle and superposition principle, shown in Figure 4, M and N are orthogonal linearly polarized light, the synthesis optical vector P will be obtained by changing the magnitude of M and N, and the amplitude and the polarization angle of the synthetic light will also be changed with the changing of M and N, thus the FSO-OFDM polarization modulation canbe progressed by using this principle [7].ωFigure 4. Optical vector compositionIII.T HE PROPAGATION CHARACTERISTIC OF POLARIZEDLASER IN ATMOSPHEREThe laser’s scattering and depolarization effects arecomplex physical phenomenon. The polarized laser’sdepolarization effect is mainly caused by the collision andscatter between the photons and the air-particles duringtransmitting in the atmosphere channel.The suspender particles and drift particles will arouse thelaser energy absorption and scatter. The reference [8] hasdescribed the scatter model of polarized laser. According tothis model, we calculated the distribution of the scatteredlaser’s polarization angle under the conditions of: the particleradius is mmmmrμμμμ8050205、、、=, the incidentpolarization angle is D0, the laser wavelength nm64.10=λ,the deflection angle D6.3=φ.0.040.080.123060901201501802102402703003300.000.040.080.120.00.10.20.30.43060901201501802102402703003300.10.20.30.4Figure.5(a) 5r mμ=Figure.5(b) 20r mμ=0.00.20.40.63060901201501802102402703003300.20.40.60.00.10.20.33060901201501802102402703003300.10.20.3Figure.5(c) 50r mμ=Figure.5(d) 80r mμ=Figure 5. Polarization angle distribution of different particle radiusFrom Figure 5 we can see that the locations with themaximum polarization angle are mainly concentrated in thebackward and lateral. We only care about the forwardscattering polarization angle in laser communication. Fromthe simulation data and simulation chart we can see clearlythat the forward scattering polarization angle is zero in theθ=0°,φ0°, namely, the spherical particles’ forwardscattering polarization angle is invariable. Many theories andpractices indicate that the air-particles are similar to sphericalparticles under the situation of which need lowprecision[9,10].According to the above analyses, it is feasible to employpolarized light to transmit signals in FSO-OFDM system.IV.FSO-OFDM SYSTEMFig.6 is the FSO-OFDM model. It is composed oftransmitting and receiving elements. The transmitter includesOFDM modulation module, optical up-converter module andatmosphere channel; the receiver includes optical down-converter module and OFDM demodulation module. Themain function of OFDM modulation module is to map thedata into OFDM symbols, then produce the baseband timeseries signals to process optical modulation. The mainfunction of optical modulation module is to linearly shift theOFDM spectrum from the RF domain to the optical domain,Figure 6. The FSO-OFDM Model(S/P—series/parallel, (I)FFT—(inverse) fast Fourier transform, GI—guardinterval, P/S—parallel/series, D/A—digital/analog, LPF—lowpass filter)The OFDM modulation module is to modulate themodulated (such as QAM, MPSK) signals onto severalorthogonal subcarriers, the baseband time series signals areas follows:()()tXNNkTsNnsmLlklmNkklm∑∑∑∝====⎟⎠⎞⎜⎝⎛=00,,1,,11ψ (3)()()()()⎪⎩⎪⎨⎧⋅+⋅+≤≤⋅⋅+=−−Δ−′else T m L l t T m L l e t S S LmT lT t Tk j k l m SS U 012,,πψ (4) Where N denotes the number of subcarriers, l denotes the number of OFDM symbols in one super-frame, m is the number of super-frames, S T , U T and Δ are the OFDM symbol period, inter-carrier distance and guard time, respectively, and satisfy Δ+=U S T T .k ′ represents the carrier index relative to the center frequency, 2/N k k −=′.k l m X ,, represents the coded signals of thelth information symbol at the kth subcarrier in mthframe.For the sake of simplicity, the OFDM signal in (5) only shows one OFDM symbol:()()UT t k j Nk k e X N t s /21,0,01Δ−′=∑=π (5) It is obvious that ()t s is the complex signal, but theoptical signals in IM/DD optical communication only can be described by intensity, that is to say the complex signals can not describe the optical signals[12]. Therefore, we propose to utilize the polarization characters of light to modulate the real /imaginary parts of the complex signals by two MZM, finally to complete the electro-optic transfer.V. THE PRINCIPLE OF FSO-OFDM POLARIZATIONMODULATION AND DEMODULATION The principle of optical modulation is shown in Fig 7. The laser signals are divided into two light beams with equal power after the beam splitter, then the two light beams passed through the polarizer 1 and polarizer 2 which are vertical each other, respectively. In this way, the two light beams will be the horizontally linearly polarized light and the vertically linearly polarized light. In this system, the RF electrical signals are divided into the real and imaginary parts to drive two MZM and obtain two optical signals. In the receiving end, two linearly light beams are obtained by holding the receiving optical signals pass through the horizontal polarizer 1 and the vertical polarizer 2. Finally, we will get the elliptical polarized light with special polarization angle after through the beam recombiner.The output signal of beam recombiner in modulation part can be written as()()()2/exp 2cos exp 2cos πωπωπππ+⋅⎟⎟⎠⎞⎜⎜⎝⎛+⋅⋅+⋅⎟⎟⎠⎞⎜⎜⎝⎛+⋅⋅=t j V V V A t j V V V A t P LD DC Q LD DC I (6) ()()()()⎩⎨⎧==t s V t s V Q I Im Re (7)A is proportional constant,,DC V is the bias voltage of themodulator, πV is the half-wave rectifier voltage, LD ωis thecarrier frequency for the laser.Figure 7. The schematic diagram of the FSO-OFDM polarizationmodulation (MZM—Mach Zehnder Modulator)Due to the horizontal polarized light and vertical polarized light are passing through the same optical path, they will with the same attenuation and delay caused by the atmospheric natural phenomenon. Hence, it will overcome the influence caused by atmosphere channel to a great extent[7].Figure 8. The schematic diagram of the FSO-OFDM polarizationdemodulationThe principle of optical demodulation is shown in Figure 8. The optical signals collected by optical antenna are divided into two-way signals after through the beam splitter, one of the signals enter into the horizontal polarization analyzer, the other into the vertical polarization analyzer. Finally, measure the horizontal polarized light intensity ∥P and the vertical polarized light intensity ⊥P by the photodetector.()()()t N t h V V V A t P DC I +⊗⎟⎟⎠⎞⎜⎜⎝⎛+⋅⋅⋅=ππρ2cos 1∥ (8) ()()()t N t h V V V A t P DC Q +⊗⎟⎟⎠⎞⎜⎜⎝⎛+⋅⋅=⊥ππρ2cos 2 (9) 1ρ,2ρare the photoelectric transformation efficient of photodector 1 and 2 respectively. The pulse response of the channel is represented by ()t h , ()t N is the Gaussian white noise with energy spectral density 0N , ()t P ⊥ and ()t P ∥ are the intensity of the vertical polarized light and the horizontal polarized light at receiver, respectively. At last, compound the signals to recover the complex OFDM signals, and then we will get the binary data after through the OFDM demodulation module.VI. CONCLUSIONIn order to analyze the performance of the light polarization FSO-OFDM system. We conduct a simulation by Monte Carlo method, and set the simulation parameters as follows: 105 OFDM symbols, the laser wavelength nm 64.10=λ, the channel is the Gaussian white noise ,the entrancing polarization angle is D 0, the number of subcarriersare N=128, 64, 32, 16, respectively. We chose PN sequence as the training sequence, and set the length of the training sequence and the length of the guard interval equal to 10 percent of the number of subcarriers. The simulation results indicated that the system’s BER is increased with the number of subcarriers increasing, but even if the number of subcarriers is 128, the system’s BER will below 10-6 when the SNR is 28dB.In this paper, we applied the OFDM technology to the free space optical communication system. According to the polarization characters of light and the influences to polarized light caused by air-particles, the air particles’ single forward scatter is invariable under the situation of which need low precision, it is feasible to employ polarized light to transmit signals in free space optical communication system. We proposed the light polarization FSO-OFDM system model, which solves the defect that the OFDM signals can not be easily practiced intensity modulation. Meanwhile, according to the simulation results the performance of the light polarization FSO-OFDM is very reliable.A CKNOWLEDGMENTThis work was supported by the National Natural Science Foundation (Grant No.60977054), The Research Foundation of Education Bureau of Shaanxi Province, China(Grant No.07JK332), The Natural Science Foundation of Shaanxi Province, China (Grant No. 2007F12). The force 863 innovation program (Grant No. 2008AAJ159 )R EFERENCES[1] Wang Huiqin, and Ke Xizheng, “Free Space OpticalCommunicationbased on Vertical Bell Labs Layered Space-Time,” Chinese Journal of Lasers, Vol.35, Jun.2008, pp.874-878. doi:10.3788/CJL 20083506. 0874[2] Shota Teramoto, and Tomoaki Ohtsuki, “Multiple-Subcarrier OpticalCommunication Systems with Peak Reduction Carriers,” GLOBECOM - IEEE Global Telecommunications Conference, IEEEpress ,Vol. E87-B, Dec.2003, pp.3274-3278. doi:10.1109/GLOCOM.2003.1258841[3] Tomoaki Ohtsuki, “Multiple-Subcarrier Modulation in OpticalWireless Communications,” IEEE Communications Magazine, Vol.41, Mar.2003, pp.74-79. doi:10.1109/MCOM.2003.1186548[4] J.Armstrong, and A.J.Lowery, “Power effcient optical OFDM,”Electronics Letters, Vol.42, Mar.2006, pp.370~372.[5] Zhang Xun, “Application of Multiple-carrier Modulation inCommunication Systems,” Optical Communication Technology, Vol.27, Otc.2003, pp. 1~2.[6] Yan Tang, William Shieh, Xingwen Yi, and Rob Evans, “OptimumDesign for RF-to-Optical Up-Converter in Coherent Optical OFDM Systems,” IEEE Photonics Technology Letters, Vol.19, Apr.2007, pp. 483-485. doi:10.1109/LPT.2007.893582[7] Ke Xizheng, Yin Zhiyun, and Yang Lihong, “Light PolarizationModulation with PPM and Its Key Technique in Atmospheric Laser Communication,” Semiconductor Optoelectronics, Vol. 28, Aug.2007, pp .553-555+560.[8] Yang Lihong, Ke Xizheng, and Ma Dongdong, “DepolarizationCharacteristics of the Polarized Laser in Atmosphere,” Opto-Electronic Engineering, Vol.38, Nov.2008, pp. 1-7.[9] Prupacher, H. R, Pitter, R.L, “A semi-empirical determination of theshape of clouds and rain drops,” Journal of Atmospheric Sciences, Vol.28, Jun.1971, pp.86-94. doi:10.1175/1520-0469(1971)028[10] Tomohiro Oguchi, “Eletromagnetic wave propagation and scatteringin rain and other hydrometerors,” Proceedings of the IEEE, IEEE press, Vol.71, Sep.1983, pp.1029-1078.[11] Ivan B.Djordjevic,Bane Vasic and Mark A.Neifeld, “LDPC codedOFDM over the atmospheric turbulence channel,” Optics Express, Vol.15, May.2007, pp.6336-6350. doi:10.1364/OE.15.006336[12] Roy You, and Joseph M.Kahn, “Upper-Bounding the Capacity ofOptical IM/DD Channels With Multiple-Subcarrier Modulation and Fixed Bias Using Trigonometric Moment Space Method,” IEEE Transactions on Information Theory, Vol.48, Feb.2002, pp. 514~523,doi:10.1109/18.979327.。

相关文档
最新文档