LC480 Software1.5中文说明书维护SOP-2009-3-10
福让冰机操作与维护手册说明书
HCC700A, HCC700W,HCD700A, HCD700W Ice Machines801 Church Lane • Easton, P A 18040, USAOperation and Service ManualOrder parts onlineTable of contentsWelcome to FollettFollett equipment enjoys a well-deserved reputation for excellent performance, long-term reliability and outstanding after-the-sale support. T o ensure that this equipment delivers the same degree of service, we ask that you review the installation manual (provided as a separate document) before beginning to install the unit. Our instructions are designed to help you achieve a trouble-free installation. Should you have any questions or require technical help at any time, please call our technical service group at (877) 612-5086 or +1 (610) 252-7301.Before you beginAfter uncrating and removing all packing material, inspect the equipment for concealed shipping damage. If damageis found, notify the shipper immediately and contact Follett Corporation so that we can help in the fi ling of a claim,if necessary.Check your paperwork to determine which model you have. Follett model numbers are designed to provide information about the type and capacity of Follett equipment. Following is an explanation of the different model numbers in the 700 series.Specifi cationsElectricalEach ice machine requires its own separate circuit with electrical disconnect within 10 ft (6m).Equipment ground required.Standard electrical:HCC700: 208-230/60/1 (6 ft (2m) NEMA 6-15 cord and plug provided)HCD700: 115/60/1 (6 ft (2m) NEMA 5-15 cord and plug provided)Maximum ice machine fuse – 15 amps eachAmperage – 115V: 10A, 220V: 5APlumbing3/8" OD push-in water inlet3/4" MPT drain1/4" FPT condenser inlet (water-cooled condenser only)1/4" FPT condenser drain (water-cooled condenser only)Notes:3/4" vented drain line must slope a minimum of 1/4" per foot (6mm per 30.4cm run).Drain to be hard piped and insulated.To prevent back fl ow, do not connect drains.Separate drains for ice machine and condenser.Water shut-off recommended within 10 feet (3m).Water supply must be treated by a scale-inhibiting fi lter (Follett item# 00130286).AmbientAir temperature 100 F/38 C max. 50 F/10 C min.Water temperature 90 F/32 C max. 45 F/7 C min.Water pressure – potable 70 psi max. (483 kPa) 10 psi min. (89 kPa)Note: Water-cooled condenser pressure 150 psi (1034 kPa)Heat rejectionAir-cooled rejects 8850 BTU/hrWater-cooled rejects 10150 BTU/hrIce productionNote: Water regulating valve set to maintain a 215 PSIG (95°F Saturated R404A) discharge pressure at all conditions.Dimensions and clearancesEntire front of ice machine must be clear of obstructions/connections to allow removal.1" (26mm) clearance above ice machine for service.1" (26mm) minimum clearance on sides.The intake and exhaust air grilles must provide at least 160 sq in (1032 sq cm) of open area.Air-cooled model HCC700A ice machines – 18" (458mm) minimum clearance between discharge and airintake-grilles.Y)3/4"1. T o clean – Remove cover. Press the CLEAN button.The machine will drain. Wait for the LO WATER lightto come on (Fig. 1).Fig. 12. Mix 1 gallon (3.8L) 120 F (49 C) water and7 ounces (198g) (one 7 ounce packet of FollettSafeCLEAN ice machine cleaner, part# 00132001).Locate cleaning cup. Fill until CLEANER FULL lightcomes on (Fig. 2).Note: Do not use bleach to sanitize or clean the icemaker.CLEANER FULL Fig. 2Fig. 33. Replace cover on cleaning cup. Wait until machinerestarts. Machine will clean, then fl ush 3 times in approximately 15 minutes (Fig. 3).4. T o sanitize – Press CLEAN button. The machinewill drain. Wait for LO WA TER light to come on(Fig. 4).CLEANER FULLFig. 5Fig. 65. Mix 1 gallon 120 F (49 C) water and 1.6 ounces(48ml) NU-CALGON IMS-II SANITIZER. Fill until CLEANER FULL light comes on (Fig. 5).Place one Sani-Sponge ™ in remaining sanitizing solution and retain for Step 9.Note: Do not use bleach to sanitize or clean theicemaker.6. Replace cover on cleaning cup. Wait until machinerestarts. Machine will sanitize, then fl ush 3 times in approximately 15 minutes (Fig. 6).Fig. 8Fig. 98. Using disposable food service grade gloves, insertdry Sani-Sponge ™ (kit part# 00132068). Next, insert Sani-Sponge soaked in Nu-Calgon IMS-II sanitizer solution (from Step 5). Push both Sani-Sponges down ice transport tube with supplied pusher tube (Fig. 8).9. Remove and discard 16" (407mm) pusher tube(Fig. 9).Fig. 11 11. Place a sanitary (2 gallon or larger) container inbin or dispenser to collect Sani-Sponges and icefor 10 minutes. Collect 5.5 lbs (3kg) of ice from unit.Discard ice and Sani-Sponges (Fig. 11).ServiceIce machine operation (all models)Follett’s ice machine consists of fi ve distinct functional systems covered in detail as follows:• Water system• Electrical control system• Mechanical assembly• Refrigeration system• Bin fullThe Horizon ice machine overviewThe Follett Horizon ice machine uses a horizontal, cylindrical evaporator to freeze water on its inner surface. The refrigeration cycle is continuous; there is no batch cycle. The evaporator is fl ooded with water and the level is controlled by sensors in a reservoir. A rotating auger (13 RPM) continuously scrapes ice from the inner wall of the evaporator. The auger moves harvested ice through the evaporator into an ice extrusion canal. The ice is forced through a restrictive nozzle that squeezes out the water and creates the Chewblet. The continuous extrusion process pushes the Chewblets through a transport tube into a dispenser or bin.A solid state PC board controls and monitors the functionality of the ice machine. In addition to sequencing electrical components, the board monitors various operational parameters. A full complement of indicator lights allows visual status of the machine's operation. Additionally, the PC board controls the self-fl ushing feature of the ice machine. The evaporator water is periodically drained and replenished to remove minerals and sediment.A unique “bin full” detection system is incorporated in the Horizon ice machine. A switch located at the ice discharge port of the machine detects the position of the transport tube. When the bin fi lls up with ice, the transport tube moves out of the normal running position, and the switch turns the ice maker off. A domed housing at the end of the transport tube contains the ice extrusion loads during shut down.Harvest system diagramElectrical systemNormal control board operationThe PC board indicator lights provide all the information necessary to determine the machine's status. Green indicator lights generally represent “go” or normal operation; Y ellow indicators represent normal off conditions; Red indicators generally represent alarm conditions, some of which will lock the machine off.A fl ashing green light labeled POWER indicates power to the machine. All other normal operation status indicators are covered as follows:DIP Switch SettingsSensor safe NOT USEDFiltration message NOT USED NOT USED60 minute time delay Flush enabledMaintenance timer OFFCapacitive touchNOT USED No filtration NOT USED NOT USED30 minute time delayFlush disabledMaintenance timer ONThe Horizon PC board monitors various operating parameters including high pressure, auger gearmotor amperage limits, clogged drain, and low water alarm conditions. There are two types of errors namely “hard” or “soft”. A hard error is one that shuts the machine off and will not allow restart until the reset button is pressed. Even cycling power will not reset a hard error. A soft error can either be automatically reset should the condition rectify, or if power is cycled. Should an error occur, consult the troubleshooting guide in this manual or a Follett service technician.Soft errors:HI AMPS: The PC board monitors the amperage of the auger motor. Should the gear motor experience current draw above the allowable limit, the machine will shut down and the TIME DELAY and HI AMP will be illuminated. After the time delay the machine will restart and the TIME DELAY and HI AMP will clear.LO WA TER: During operation, the water level cycles between the normal low and normal high sensors. Should the water be shut off to a running machine, a soft error will occur. The error sequence is as follows: During operation, the water level falls to the normal low sensor, and when it does the water feed solenoid is energized. If water is not detected at the normal low sensor within 10 seconds, a soft error will occur. The machine will shut down, but the water feed solenoid will remain energized. Should water return, it will fi ll to the normal low sensor and the machine will resume normal operation. The error will clear automatically.HI PRESSURE: Should the refrigeration pressure rise above 425 psi, the machine will shut down and the TIME DELAY and HIGH PRESSURE will be illuminated. After the time delay, and if the pressure has fallen back below the reset point of 295 psi, the machine will restart and the TIME DELAY and HIGH PRESSURE will clear.Hard error:DRAIN CLOG: The drain clog sensor, located in the chassis, underneath the rear drain pan, will detect the presence of water just below the top edge of the pan. If water does not properly fl ow out of the drain pan it will overfl ow into the chassis and rise to the sensor (especially during a self-fl ushing purge cycle). Turning the power switch off and then back on again will restart the ice machine.Relay output indication:Each relay on the board has an indicator light associated with its output. For example, when the relay for the water feed solenoid is energized, the adjacent indicator light glows green.Time delay on start-up:ATTENTION!Unit has 15 minute start up delay when power is applied toprevent circuit overload. Depressing the reset button on thecontrol board will bypass this delay.Flushing logicFlush on fl y: For every one (1) hour of ice making time, the machine will open the drain valve for a duration of 60 seconds. While the drain valve is open, the machine will continue to make ice and the water feed valve will cycle to maintain water level.Off cycle: At the completion of off-cycle time delay, the machine checks for a cumulative one (1) hour of ice making time since the last off-cycle fl ush. If the cumulative ice making time exceeds one (1) hour, the machine will open the drain valve for 60 seconds to drain the evaporator in its entirety. It will then refi ll with water and begin making ice. If the ice making time is less than 1 hour, the machine will start and begin making ice without draining the evaporator.Mechanical SystemFig. 12Evaporator disassembly1.power OFF when LO WA TER lights.2. louvered docking assembly.3.Unplug gear motor.4. Remove shuttle housing:§Remove vent tube (Fig. 14.1).§Disconnect shuttle housing switch connections (Fig. 14.2).§Remove two screws and lift shuttle housing (Fig. 14.3).Fig. 15 5. Remove gear motor:§Remove gear motor insulation (Fig. 15.1).§Remove 11/16" auger nuts and washer (Fig. 15.2).§Remove two 1/2" bolts (Fig. 15.3).§Pull gear motor from auger (Fig. 15.4).§Remove main housing insulation (Fig. 15.5).6. Remove all traces of petro-gel from auger shaft.7. Remove main housing:§Use an allen wrench to remove 3/16" allen screws (3) (Fig. 16.1).§Remove shaft insulation (Fig. 16.2).§Remove main housing (Fig. 16.3).8. Remove and discard mating ring and seal(Fig. 17.1).9. Carefully remove auger (Fig. 17.2).ARNING!very sharp - handle with care to avoid personalinjury.10. Press the lever on the back of the reservoir(Fig. 18.1) to release and remove the solenoid (Fig. 18.2).11. Remove three screws to remove the reservoirinsulation (Fig. 19).12. Remove three screws to remove the reservoir(Fig. 20).13. T o remove the rear bushing, place the auger into theevaporator and use it to gently tap and dislodge therear bushing housing (Fig. 21).Evaporator reassembly Fig. 22 1. Remove and inspect O ring seal. Replace ifdamaged in any way.2. Place rear bushing into evaporator.3. Install the three bolts: partially tighten each bolt,alternating bolts until the rear bushing is fully seatedand properly aligned (Fig. 22).4. When fully seated, remove the bolts.5. Install the reservoir with three bolts (Fig. 23).6. Install the reservoir insulation, tube clamp, andsolenoid lever with three screws.7.Install solenoid.1. Remove and inspect O ring seal. Replace ifdamaged in any way (Fig. 25.1).2. Clean O ring groove. Lubricate O ring with petrol-geland reinstall (Fig. 25.2).3. Use cardboard disc to press new mating ring intomain housing (Fig. 26.1).4. Lube the shaft with liquid soap in the area shown(Fig. 26.2) and slip on seal and spring (Fig. 26.3).Note: Do not touch the sealing surfaces with barehands. Contact with bare skin will cause premature seal failure.5. Install auger (Fig. 26.4).6. Install main housing:§Slide main housing onto auger shaft (Fig. 27.1).§Install main housing insulation (Fig. 27.2).§Use an allen wrench to install 3/16" allen screws (3) (Fig. 27.3).7. Apply a coat of petrol-gel to the auger shaft.8. Install gear motor:§Install main housing insulation (Fig. 28.1).§Slide gear motor onto the auger shaft (Fig. 28.2).§Install two 1/2" bolts (Fig. 28.3).§Install 11/16" auger nuts and washer (Fig. 28.4). 9. Use 3/16" allen wrench to orient auger shaft withkeyway in the upward position (Fig. 29.1).10. Install key into keyway (Fig. 29.2).11. Install washer and fi nger tighten 11/16" jam nut.12. Tighten jam nut with adjustable wrench while usingallen wrench to prevent auger from turning.13. Install and tighten 11/16" auger nut with adjustablewrench while using another adjustable wrench tohold jam nut.14. Install gear motor insulation.Fig. 3215. Install shuttle housing:§(Fig. 32.1).§Plug in shuttle housing connections §Connect vent tube (Fig. 32.3).16. Plug in gear motor.§BROWN to BLUE §BLACK to BLACK §WHITE to GRAY17. assembly.Refrigeration system Refrigerant pressure dataRefrigeration system diagramRefrigeration chargeAll service on refrigeration systems must be performed in accordance with all federal, state and local laws. It is the responsibility of the technician to ensure that these requirements are met. Recharging ice machine to other than factory specifi cations will void the warranty.Refrigerant replacement requirements1. Non-contaminated refrigerant removed from any Follett refrigeration system can be recycled and returned tothe same system after completing repairs. Recycled refrigerant must be stored in a clean, approved storage container. If additional refrigerant is required, virgin or reclaimed refrigerant that meets ARI standard 700-88 must be used.2. In the event of system contamination (for example, a compressor burn out, refrigerant leak, presence ofnon-condensibles or moisture), the system must be repaired, evacuated and recharged using virgin orreclaimed refrigerant that meets ARI standard 700-88.3. Follett Corporation does not approve of recovered refrigerants. Improper refrigeration servicing procedureswill void the factory warranty.EvacuationEvacuate the system to a level of 500 microns. When the 500 micron level is reached, close all valves. Allow the system to sit for approximately 20 minutes. During this period the system pressure should not rise. If the system pressure rises and stabilizes there is moisture in the system and further evacuation is needed. If the pressure continues to rise check the system for leaks.Ambients MinimumMaximumAir temperature150 F/10 C 100 F/37.8 CWater temperature245 F/7 C 90 F/32.2 C1Ambient air temperature is measured at the air-cooled condenser coil inlet.2Ambient water temperature is measured in the ice machine water reservoir.Ice capacity testIce machine production capacity can only be determined by weighing ice produced in a specifi c time period.1. Replace all panels on ice machine.2. Run ice machine for at least 15 minutes.3. Weigh and record weight of container used to catch ice.4. Catch ice for 15 or 20 minutes.5. Weigh harvested ice and record total weight.6. Subtract weight of container from total weight.7. Convert fractions of pounds to decimal equivalents (ex. 6 lbs 8oz = 6.5 lbs).8. Calculate production using following formula:9. Calculated amount per 24 hours should be checked against rated capacity for same ambient and watertemperatures in Ice Production Tables.“Bin full” detection systemThe Follett Horizon ice machine incorporates a unique “bin full” detection system that consists of the shuttle and actuator. The shuttle incorporates a fl ag and sensor. Referencing the fi gure below, the normal running position of the fl ag is down, out of the sensor. When the bin fi lls to the top and ice can no longer move through the tube, the machine will force the shuttle fl ag up into the sensor, shutting the machine off. The shuttle actuator, located above the ice bin allows the ice to curl up within it when the bin is full. In this way, there are no loads generated that would tend to lift off the lid of the bin.Shuttle fl ag and sensorShuttle actuatorTroubleshootingPlease see “Service” section for a description of each function.Order parts online Order parts online Water-cooled assembly Order parts onlineOrder parts online Order parts online Integration kit – top-mount and RIDE remote ice delivery Order parts onlineOrder parts online Horizon, Harmony, Ice Manager, SafeCLEAN, Sani-Sponge, RIDE and Vision are trademarks of Follett Corporation. Chewblet and Follett are registered trademarks of Follett Corporation, registered in the US.。
neutrino 480M操作手册
480M Series Loudspeaker Management SystemUser ManualXilica Audio DesignImportant Safety Instructions1. READ THESE INSTRUCTIONSAll the safety and operating instructions should be read before the product is operated. 2. KEEP THESE INSTRUCTIONSThe safety and operating instructions should be retained for future reference.3. HEED ALL WARNINGSAll warnings on the product and in the operating instructions should be adhered to.4. FOLLOW ALL INSTRUCTIONSAll operating and use of instructions should be followed.5. DO NOT USE THIS APPARATUS NEAR WATERDo not use the product near water. For example, near a bathtub, washbowl, kitchen sink, or laundry tub, in a wet basement, or near a swimming pool, and the like.6. CLEAN ONLY WITH DRY CLOTHUnplug the unit from the wall outlet before cleaning. Do not use liquid cleaners or aerosol cleaners. Use a damp cloth for cleaning.7. DO NOT BLOCK ANY VENTILATION OPENINGSSlots and openings in the cabinet back or bottom are provided for ventilation, to ensure reliable operation of the limit and to protect it from overheating. These openings must not be blocked or covered. The openings should never be blocked by placing the product on a bed, sofa, rug, or similar surface. This product should never be placed near or over a radiator or heat source. This product should not be placed in a built-in installation such as a bookcase or rack unless proper ventilation is provided or the manufacture's instructions have been adhered to.8. DO NOT INSTALL NEAR ANY HEAT SOURCESThis Product should be situated away from heat sources such as radiators, stoves, orother products (including amplifiers) that produces heat.9. DO NOT DEFEAT THE SAFETY PURPOSE OF THE POLARIZED OR GROUNDING-TYPE PLUGA Polarized plug has two blades with one wider than the other. A grounding-type plug hastwo blades and a third grounding prong. The wide blade or the third prongs are providedfor your safety. If the provided plug does not fit into your outlet, consult an electrician forreplacement of the obsolete outlet.10. PROTECT THE POWER CORD FROM BEING WALKED ON OR PINCHED PARTICULARLY AT PLUGS, CONVENIENCE RECEPTACLES, AND THE POINT WHERE THEY EXIT FROM THE APPARATUS.11. ONLY USE ATTACHMENTS/ACCESSORIES SPECIFIED BY THE MANUFACTURER.12. USE ONLY WITH CART, STAND, TRIPOD, BRACKET, OR TABLE SPECIFIED BY THE MANUFACTURER, OR SOLD WITH THE APPARATUS. WHEN A CART IS USED, USE CAUTION WHEN MOVING THE CART/APPARATUS TO AVOID INJURY FROM TIP-OVER. Do not place this unit on an unstable cart, stand, tripod, bracket, or table. The unit mayfall, causing serious injury to someone, and serious damage to the appliance. A unit andcart combination should be moved with care. Quick stops, excessive force, and unevensurfaces may cause the product and cart combination to overturn.13. UNPLUG THIS APPARATUS DURING LIGHTNING STORMS OR WHEN UNUSED FOR LONG PERIODS OF TIME.For added protection for this unit during a lightning storm, or when it is left unattendedand unused for long periods of time, unplug it from the wall outlet and disconnect theantenna or cable system. This will prevent damage to the unit due to lightning and powerline surges.14. REFER ALL SERVICING TO QUALIFIED SERVICE PERSONNEL. SERVICING IS REQUIRED WHEN THE APPARATUS HAS BEEN DAMAGED IN ANYWAY, SUCH AS WHEN THE POWER SUPPLY CORD OR PLUG IS DAMAGED, LIQUID HAS BEEN SPILLED OR OBJECTS HAVE FALLEN INTO THE APPARATUS, THE APPARATUS HAS BEEN EBSOSED TO RAIN OR MOISTURE, DOES NOT OPERATE NORMALLY, OR HAS BEEN FROPPED.15. WARNING: TO REDUCE THE RISK OF FIRE OR ELECTRIC SHOCK, DO NOT EBSOSE THIS APPARATUS TO RAIN OR MOISTURE.16. APPARATUS SHALL NOT BE EBSOSED TO DRIPPING OR SPLASHING AND NO OBJECTS FILLED WITH LIQUIDS, SUCH AS VASES, SHALL BE PLACED ON THE APPARATUS.Table of ContentsTable of Contents (4)1.0 Introduction (5)2.0 Features (6)3.0 Front Panel Functions (7)4.0 Rear Panel Functions (9)5.0 Powering Up the Device (10)6.0 Operating the Device (11)7.0 Quick Reference (19)8.0 PC Control Software (20)9.0 Specifications (21)10.0 Warranty (24)1.0 IntroductionThe 480M is a complete 4 input - 8 output digital loudspeaker management system designed for the touring or fixed sound installation markets. The absolute latest in available technology is utilized with 32-bit (40-bit extended) floating point processors and high performance 24-bit Analog Converters. The high-bit DSP prevents noise and distortion induced by truncation errors of the commonly used 24-bit fixed-point devices. A complete set of parameters include I/O levels, delay, polarity, 6 bands of parametric EQ per channel, multiple crossover selections and full function limiters. Precise frequency control is achieved with its 1 Hz resolution. Inputs and outputs can be routed in multiple configurations to meet any requirement. The 480M can be controlled or configured in real time onthe front panel or with the intuitive PC GUI accessed via the RS-232 interface. Software upgrade for CPU and DSP via PC keeps the device current with newly developed algorithms and functions once available. Multiple setup storage and system security complete this professional package.Shipped contents:- 480M Unit- User Manual- Xlink-Software- Power Cord2.0 Features> > > > > > > > > > > > > > > > 4 Inputs and 8 Outputs with flexible routing32-bit (40-bit extended) floating point DSP96kHz Sampling Rate SelectableHigh Performance 24-bit A/D Converters1 Hz Frequency Resolution6 Parametric Equalizers for each Input and Output Multiple Crossover types with Full Function Limiters Precise Level, Polarity and DelayCPU and DSP upgrade via PCIndividual Channel Buttons with Linking capability 2-Line x 16 Character Backlit LCD DisplayFull 5-segment LED’s on every Input and Output Storage of up to 30 Preset SetupsMultiple Levels of Security LocksRS-232 Interface for PC Control and Configuration Future options available3.0 Front Panel Functions1. Mute keys - Mute/Unmute input and output channels. When an input channel is muted, a red LED will come on for indication.2. Peak Level LED - Indicates the current peak level of the Signal:Signal, -12dB, -6dB, -3dB, Over/Limit. The Input Over LED references to the device's maximum headroom. The Output Limit LED references to the threshold of the limiter.3. LCD - Shows all the necessary information to control the unit.4. Menu Control keys - There are 6 menu keys: <<Menu (Menu Down), Menu>> (Menu Up), <<Cursor (Cursor Down), Cursor>> (Cursor Up), Enter/Sys/Speed and Exit . The functions of each key is explained below:<<Menu: Previous menu screenMenu>>: Next menu screen<<Cursor: Previous cursor in the menu screenCursor>>: Next cursor in the menu ScreenEnter/Sys: Enter is used only in the System Menu to proceedwith selected actionsSys enters the System Menu from the main menu Speed modifies delay and frequency (1 Hz resolution mode) data values by 100X.Exit: Exit to the Main Menu5. Rotary Thumb Wheel - Changes parameter data values. The wheel has travel velocity sensing which ease large incremental data modifications. For modifying delay and frequency (1 Hz resolution), pressing the Speed key simultaneously will increment/decrement the data value by 100X.4.0 Rear Panel Functions1.Main Power - Connects via a standard IEC socket. A compatible power cord is supplied with the unit. The voltage input is either 115VAC or230VAC and is clearly specified on the unit. Voltage requirement has to be stated upon ordering.2. Main Fuse - T200mA-250V. Slow blow type.3. Power switch - Controls power On/Off.4. RS232 - a standard female DB9 socket. A straight through cable is required for PC connection.5. XLR input and outputs - Separate 3-pin XLR connectors are provided for each audio input and output. The device's output stage employs the balanced impedance topology. All I/O connectors have pin 1 as ground (shield), pin 2 as + and pin 3 as -.5.0 Powering Up the Device•After powering up the unit, the following initialization screen is displayed on the LCD:NEUTRINO480 Ⅶ9.05•The initialization process takes several seconds and during that period the unit boots and displays the device model and firmware version. •After the initialization process is finished the 480M displays its main screen:NEUTRINOP01*•The screen shows the current program number and program name assigned to the unit. The program assigned is always the last program the user recalled or stored before powering down the unit.•Now the 480M is ready to operate.6.0 Operating the DeviceTips: Channel Linking - If the user presses one of the Input or Output Menu keys, holds it down and press any other Menu key(s) in the same group (Input or Output group), then the channels are linked together. The green menu LEDs for the linked channels are lit. Any modification of the data for the selected channel will be applied to the linked channels as well. To cancel the linking, just press any other Menu key or the Sys key after releasing the held key.6.1Input menusEach of 480M input channels has a separate Menu key. There are 3 menus for each input channel.Signal - Signal parametersLEVEL - Gain, -40.00dB to +15.00dB in 0.25dB steps.POL - Polarity, can be normal (+) or inverted (-).DELAY - Delay in 21us steps. Can be displayed in ms, ft or m. Thetime unit of the delay can be changed in the System menu. The maximum delay permitted is 21,600 steps (450ms).EQ - EQ parametersEQ# - Selects one of the 6 available Equalizers.LEVEL - EQ level gain. Ranges from -30.00dB to +15.00dB in0.25dB steps.FREQ - EQ center frequency. Ranges from 20 to 20,000Hz in either 1Hz steps or 1/36 octave steps. The frequency steps can be selected in the System Menu.BW - EQ Bandwidth. Ranges from 0.02 to 2.50 octaves in steps of 0.01 octave steps for PEQ. The Q value is automatically shown beneath the octave value. For Lo-Slf or Hi-Shf, it is either 6 or12dB/Oct.Type - Type of EQ. The types can be parametric (PEQ), Lo-shelf (Lo-shf) and Hi-shelf (Hi-shf).Name - Channel name. It is 6 characters in length.6.2 Output MenusEach output channel of the 480M has a separate menu key. There are 6 menus for each output channel.Signal - Signal parameters Refer to the Input Menus for detailsEQ - EQ paramtersRefer to the Input Menus for detailsX’Over - Crossover parametersFTRL - Filter Type of low frequency crossover point (high pass). Types can be Buttwrth (Butterworth), Link-Ri (Linkritz Riley) or Bessel.FRQL - Filter cut-off Frequency of low frequency crossover point (high pass). Ranges from 20 to 20,000Hz in either 1Hz steps or 1/36 octave steps. The frequency steps can be selected in theSystem Menu .SLPL - Filter Slope of low frequency crossover point (high pass). Ranges from 6 to 48dB/octave in 6dB/octave steps. If the selected Filter Type is Linkritz Riley, the available slopes are 12 / 24 / 36 / 48 dB/octave.FTRH - Filter Type of high frequency crossover point (low pass).FRQH - Filter cut-off Frequency of high frequency crossover point (low pass).SLPH - Filter Slope of high frequency crossover point (low pass).Limit - Output LimterTHRESH - Limit Threshold. Ranges from -20 to +20dBu in 0.5dB steps.ATTACK - Attack time. Ranges from 0.3 to 1ms in 0.1ms steps,and ranges from 1 to 100ms in 1ms steps.RELEASE - Release time. Can be set at 2X, 4X, 8X, 16X or 32Xthe attack time.Source - Input Source1,2,3– Input channel source for the current output channel. Can be set to enable the input source (On) or disable it (Off). If more than one input source are enabled, they will be added together asthe source for the current output channel.Ch-Name - Channel Name6.3System MenusThe System Menus allow the user to control and change parameters that are related to the system behavior and general operation. It can be accessed by pressing the Sys key in the main menu (when no Input/Output or System Menu is activated). All System Menus require the Enter key to be pressed for the selected action.Recall - Program RecallThe 480M has a built in non-volatile memory that can store up to 30 different program setups. A program can be recalled using this menu. PROG - Program Number to be recalled..Program Name of the program. This is read only, the userhas no access to them.Store - Program storeThe 480M has a built in non-volatile memory that can store up to 30 different program setups. A program can be stored using this menu. The old program with the same program number will be replaced. Once the program is stored in the flash memory, it can be recalled at a later time, even after power down.PROG - Program Number for the current data to be stored.NAME - Program Name, allows a maximum length of 12characters.Copy - Copy channelsCopy Channels from the source to the target. When the Source and Targets are both Inputs or Outputs, all audio parameters will be copied. When one of the Source or the Target is an input while the other is an output, only the Level, Polarity, Delay and EQ are copied.SOURCE - Channel to be copied from.TARGET - Channel to be copied to.General - General system parametersFREQ MODE - Selects the frequency control mode for EQ and crossover filters. Can be 36 steps/octave or All Frequencies (1 Hz resolution).DELAY UNIT - ms, ft or m.DEVICE# - Assigns the device ID from 1 to 16. This ID is useful when a network of more than 1 unit is present.Security - Security LocksThe 480M enables the user to secure the unit and prevent undesired changes in the setup. In order to lock/unlock the unit the user must enter the correct password.PASSWORD – The password of the 480M is 4 characters in length. The user can change it via the PC application software. Thefactory default of a new unit does not require a password.7.0 Quick Reference8.0 PC Control SoftwareThe 480M is shipped with a special PC Graphic User Interface (GUI) application - XLink. XLink gives the user an option to control the 480M unit from a remote PC via the RS232 serial communication link. The GUI application makes it much easier to control and monitor the device, allowing the user to get the whole picture on one screen. Programs can be recalled and stored from/to PC’s hard drive, thus expanding the storage to become virtually limitless.9.0 SpecificationsInputs and OutputsInput Impedance: >10k OhmsOutput Impedance: 50 OhmsMaximum Level: +20dBuType: Electronically balancedAudio PerformanceFrequency Response: +/- 0.1dB (20 to 20kHz) Dynamic Range: 115dB typ (unweighted) CMMR: > 60dB (50 to 10kHz) Crosstalk: < -100dBDistortion: 0.002% (1kHz @+4dBu)Digital Audio PerformanceProcessor: 32-bit (40-bit extended) Sampling Rate: 48kHzAnalog Converters: High Performance 24-bit Propagation Delay: 3msFront Panel ControlsDisplay: 2 x 16 Character Backlit LCD Level Meters: 5 segment LEDButtons: 9 Mute Controls9 Gain/Menu Controls6 Menu ControlsDial Encoder: Embedded Thumb WheelConnectorsAudio: 3-pin XLRRS-232: Female DB-9Power: Standard IEC SocketGeneralPower: 115 / 230 VAC (50 / 60Hz) Dimensions: 19”x2.45”x8” (483x88x203 mm) Weight: 9.47lbs / 4.295kgAudio Control ParametersGain: -40 to +15dB in 0.25dB steps Polarity: +/-Delay: Up to 50ms per I/OEqualizers (6 per I/O)Type: Parametric, Hi-shelf, Lo-shelf Gain: -30 to +15dB in 0.25dB steps Bandwidth: 0.02 to 2.50 octaves (Q=0.5 to 72)Crossover Filters (2 per Output)Filter Types: Butterworth, Bessel, Linkwitz Riley Slopes: 6 to 48dB/octLimitersThreshold: -20 to +20dBuAttack: 0.3 to 100msRelease: 2 to 32X the attack timeSystem ParametersNo. of Programs: 30Program Names: 12 character lengthDelay Units: ms, ft, mFrequency Modes: 36 steps/oct, 1Hz resolution Security Lock: Lock/UnlockCopy channels: All parametersChannel Names: 6 character lengthNote: Specifications subject to change without notice10.0 WarrantyThe 480M is warranted covering materials and workmanship for a period of one (1) year, as determined by the date of retail purchase (according to the sales receipt from an authorized dealer) or the date of manufacture if the sales receipt is not available (according to the serial number). This warranty applies to the product; therefore, the remainder of the warranty period will be automatically transferred to any subsequent owner. This warranty applies only to failure of a PSE product caused by defects in materials and workmanship during the stated warranty period. It does not apply to a unit that has been subjected to abuse, accident, modification, improper handling/installation, or repairs made without factory authorization or by anyone other than authorized PSE Field Service Stations. This warranty is void if the serial number has been defaced, altered or removed. Products covered by this warranty will be repaired or replaced at the option of PSE, without charge for materials or labor, provided all the terms of this warranty have been met.For factory service, please call or email for a Return Authorization (R/A) number before shipping. If the product is shipped, the following information must be included in the package:1. Owner’s complete name, daytime phone numb er, return street address and return authorization number.2. The serial number of the product being returned and a copy of the retail sales receipt, if possible.3. A complete description of the problem(s) experienced, including a brief description of how the equipment is being used and other equipments involved.。
LC480操作与维护规程
LC480操作与维护规程一、操作规程1.1系统启动与关闭1.1.1确保设备连接到电源插座，并检查电源线是否完好无损。
1.1.2按下电源开关，等待设备启动。
在启动过程中，请勿进行其他操作。
1.1.3在操作系统完全启动后，可进入设备的操作界面。
1.1.4关闭设备时，点击操作界面上的关机按钮，等待系统关闭后，再切断电源。
1.2样品的准备1.2.1根据实验要求，准备好需要检测的样品。
1.2.2样品处理过程中应注意避免污染和混入异物。
1.2.3根据实验要求，将样品放置在适当的容器中，以便放入设备中进行测试。
1.3样品放置与读取1.3.1打开设备上的样品舱门，将准备好的样品放入样品舱中。
1.3.2关闭样品舱门，确保样品舱门封闭严密。
1.3.3在操作界面上选择相应的测试方法和参数。
1.3.4点击开始按钮，设备将自动进行测试，过程中请勿移动或震动设备。
1.3.5测试完成后，再次打开样品舱门，取出已测试过的样品。
1.4设备的维护1.4.1定期检查设备的电源线、连接线等是否完好无损。
1.4.2使用干净、柔软的布进行设备的清洁，特别是外壳和显示屏。
1.4.4定期检查设备的耗材（如PCR管、滤膜、耗材盒）是否足够，并根据需要进行更换。
1.4.5设备长时间不用时，应切断电源，并盖上防尘罩。
二、维护规程2.1硬件维护2.1.1清洁外壳：使用柔软的布擦拭设备的外壳，注意避免使用含有酒精和腐蚀性物质的清洁剂。
2.1.2清洁显示屏：使用干净、干燥的布轻轻擦拭显示屏，避免使用力过大，以避免刮伤屏幕。
2.1.3检查连接线：定期检查连接线是否松动或磨损，如有需要，及时更换连接线。
2.1.4检查电源线：定期检查电源线是否有损坏现象，如有需要，及时更换电源线。
2.2软件维护2.2.1定期更新软件：根据设备提供的更新提示，定期更新设备的软件版本。
2.2.2备份数据：定期备份设备中的重要数据，以避免数据丢失。
2.2.3定期校准设备：根据设备的校准指南，定期对设备进行校准，以确保测试结果的准确性。
LC480-Software1.5中文说明书维护SOP-2009-3-10
LightCycler480荧光定量PCR仪操作规程1．每次反应最后一步都必须做降温，降至40度，维持至少30秒。
2．在操作过程中，切忌戴有粉末的乳胶手套接触光学透性封板膜。
3．试剂加完样，封膜完毕后，在板式离心机中1500×g（3000rpm）离心两分钟，以排除气泡。
4．先开电脑在开仪器，先关仪器再关电脑。
打开总电源，打开连接LightCycler480荧光定量PCR仪的电脑，并打开LightCycler480 Software软件，然后打开LightCycler480仪器后方的电源开关，此时仪器会在与电脑软件接通后进行自检，仪器正面右边两盏桔黄色的灯会闪烁。
自检完毕后，左边的灯会变绿，右边的灯仍为桔黄色。
这时按一下灯右边带双箭头的开关，仪器的载板器会自动伸出，将准备好的反应板放入载板器中（反应板的切角方向应与载板器的切角方向相应才能将反应板正确放入，切勿用裸手接触反应板的光学透性封板膜）。
再按一下带双箭头的开关，仪器的载板器会自动缩入仪器内，此时仪器能自动检测到反应板，原来呈桔黄色的灯会变绿，LightCycler480 Software中Start Run按钮也同时变为激活状态。
5．设定完实验程序并编辑完样本后，点击软件中Start Run按钮，运行实验。
6．软件界面上出现Run Complete….标识时，说明实验运行完毕，按一下仪器上带双箭头的按钮，使载板器伸出，取下反应板，再按一下带双箭头的按钮，使载板器缩回，关闭LightCycler480仪器后方的电源开关，在电脑上分析实验数据。
7．分析完实验数据后保存结果，打印报告，并关闭电脑，关闭总电源。
实验员在离开PCR扩增区时带走用完的反应板。
维护SOPLightCycler 480是一台免维护的仪器。
做任何维护工作前，须确认主机电源已被关闭。
预防性维护：1．检查LightCycler 480主机两侧与背后空间，确保无任何阻碍空气流通的物品，包括书本、纸张或其他任何物品；2．若主机表面和桌面有灰尘，用不起毛的软布蘸少量清水擦拭干净。
LightCycler480快速操作指南
一．前期准备：配制好反应体系，封好膜。
接通LC480与电脑电源，电脑帐号operator，密码LC480，点击LC480软件，登录帐号user，密码Master1。
二．开始实验：点击，在列表中选择对应的程序，如H1N1或HBV，点击窗口右下角的，点击软件界面右下角的，输入实验文件名点击窗口右下角的开始实验。
三．编辑子集：点击subset editor，点击左下角的，按ctrl键的同时鼠标选择本次实验的孔，最后点击应用。
注意，一次实验可以同时运行相同扩增参数的多个实验（如H1N1、HIV与HCV），那么可以分别设置多个子集设置样品：先在subset中选择本次实验的子集，点击左上角的，样品输入样品名，再选择标准品类型和浓度或者阳性对照/阴性对照四．数据分析：实验运行完成后进入，选择Abs Quant/Fit Ppoint，在窗口中的Subset（第二行）中选择本次实验的子集，点击软件界面中上方的，在Noise Band中调节noiseband高度，使之处于对数增长期并高于所有噪音信号，如右图。
再点击进入，点击软件界面中间偏右的再点击软件界面左下角的值或浓度值等以及平均值标准误等信息，该部分可以鼠标拖动滑块或鼠标拖曳、鼠标点击软件右上角的为本次分析命名，如输入H1N1或其它名称五．报告输出打印：点击软件右侧的保存当前实验的分析结果，再点击软件左侧的，点击软件中部左侧的detailed 选择需要输出的分析结果，如H1N1，分析中建议选择results或standard curve，点击软件界面左下方的，生成PDF报告，如装了打印机，直接点击软件中间上方的，或者点击，选择pdf文件保存位置后点击save保存六．维护保养•环境要求LC480 UPS≥2500 V A，配备有良好的接地线•实验室温度：20 ±5℃；实验室湿度：20% - 80%•实验室配备有空调做温度及湿度的调控•3~6个月清洗或更换防尘滤网•实验数据建议定期备份到D盘•仪器避免阳光直射，也避免处在强电磁干扰或通风不畅等环境中七．售后支持•若LC480开机自检后第一个指示灯显示为红色，请立即直接致电400-820-8864，将软件下方的错误代码报给Roche公司维修人员，以确定故障类型，便于后续维修工作•如遇到任何软件使用问题，可以参考查询D盘的电子版中文说明书，也可直接致电800-820-0577或021-********•实验数据建议定期备份到D盘。
格林威尔开通维护指导书(最终版)
检查板卡lpt是否使能若使能则检查对接设备的电口光口是否连接正常对接设备运行状态是否正常对接设备配置是否正检查电口以太网设置是否正确可通过拨码开关设置电口状态多业务接入设备msape3300a31设备简介msape3300a整机如图31前视和图32后视所示其采用19英寸宽3u高插卡式结构最多可以插17张盘其中最右侧的两个插卡位置是电源盘的槽位紧靠着电源盘位置的是主控盘的槽位左侧的1431e3300a前面板示意图32e3300a后面板示意图220v12
□盘运行指示(RUN)：无主控盘在位时当盘正常运行后，此绿灯闪亮；
□总告警指示(ALARM)：当板卡运行时出现任意告警，此红灯常亮；
□环回指示(LOOP)：当板卡内部设置环回时，此红灯常亮；
□RJ45接头左下角灯（LINK/ACT）：亮表示以太网电口有连接，灭表示没有连接，闪亮表示有数据传送；
□RJ45接头右下角灯(10/100M)：亮表示速率为100M，灭表示10M；
E31-EC41-FE盘指示灯：
□盘运行指示(RUN)：无主控盘在位时当盘正常运行后，此绿灯闪亮；
□总告警指示(ALARM)：当板卡运行时出现任意告警，此红灯常亮；
□环回指示(LOOP)：当板卡内部设置环回时，此红灯常亮；
□RJ45接头左下角灯(LINK/ACT)：亮表示以太网电口有连接，灭表示没有连接，闪亮表示有数据传送；
□PGND为电源保护地接线端。
1.2.1.1交流电源线的连接
电源接口位于机箱后面板。交流220V电源接口使用标准的三端带开关保险丝的电源插座，可直接使用标准的220V电源线。
□AC220V电源接口：直接使用附件中提供的三相交流电源线；
兰德华软件使用说明
L-A1.0软件使用说明为了能够使您尽享本系统操作的方便快捷，请您详细阅读本说明：一、系统安装说明：打开您的电脑，启动操作系统之后，将本系统光盘放入光驱，打开我的电脑，打开光盘，会显示带有软件名称的文件夹，双击打开该文件夹，点击运行SETUP文件，您就可以根据中文提示安装本系统了。
注：如软件安装过程中出现如下提示，请直接点击取消按钮。
此操作不影响软件的正常安装使用。
若您使用的是通过USB口传输的巡检器，请您在安装完本软件系统后再安装USB驱动，详细的安装程序请参考USB驱动安装说明。
二、软件操作说明：（一）软件登录、注销1、登录双击巡更管理系统图标，软件起动后，会出现以下登录界面，选择权限，然后输入相对应的密码，系统管理员初始密码为333；操作员密码为222；浏览者密码为111。
只要输入正确，便可登录成功；登录界面如下图：登录成功后进入软件主窗口，提示选择您所使用的产品型号，如图：请在此选择用户名请在此输入密码2、注销操作员的注销，即更换操作员，使新的操作员用新的权限登录。
注销方法（如下图），选择菜单“操作员 用户注销”来实现。
点击此处即可（二）资源设置资源设置分五部分组成：人员钮设置、地点钮设置、事件设置、棒号设置、系统设置，每一部分的设置如下：1、系统设置：在第一次进入软件后，应首先对系统进行设置。
系统设置分为基本信息写入和权限用户密码管理。
如下图，在此可输入公司名称、选择的串口号，并可在此对权限密码进行修改。
修改完毕点击保存即可。
在此修改密码注：巡检器与电脑是用串口进行数据传输的，默认使用的串口号为COM1，巡检器与电脑是用USB 口传输的，默认使用的串口号为COM3（具体情况可到设备管理器中查询），在系统设置完毕后请重新登录巡更系统。
2、棒号设置：在使用巡检器之前需要将巡检器的棒号输入到软件中，以便识别。
点击“资源设置—>棒号设置”。
将巡检器与计算机连接好，并且将巡检器打开。
点击采集数据，会出现如下图所示：1140为该棒号码。
alliance 洗脱机 固定式机体 安装操作保养手册说明书
汞 （Hg）
镉 （Cd）
六价铬 （CR[VI]）
聚溴联苯 （PBB）
聚溴二苯醚 （PBDE）
PCBs
X
O
O
O
O
O
机电部件
O
O
O
O
O
O
电缆和电线
O
O
O
O
O
O
金属部件
O
O
O
O
O
O
塑料部件
O
O
O
O
O
O
电池
O
O
O
O
O
O
软管和管道
O
O
O
O
O
O
同步带
O
O
O
O
O
O
绝缘材料
O
O
O
O
O
O
玻璃
O
O
O
O
O
O
显示
O
O
O
O
O
• 请勿在洗涤剂盒的喷嘴（如适用）上装贴任何物体。必 须为此保留空气间隙。
• 如洗衣机上未安装循环水塞或循环水系统（如适用）， 则不可操作本机。
• 请确保在水管连接时安装截止阀，进水管间连接紧密。 在结束清洗的当天，务必关闭截止阀。
• 请妥善保养洗衣机。如洗衣机遭撞击或发生坠落，安全 功能可能会受到损坏。如发生此类情况，请联系合格服 务人员检查机器。
重要安全说明
警告
在使用洗衣设备时，为降低发生火灾、触电、人员重伤或 死亡等风险，请务必遵守这些基本注意事项：
W023
• 请在使用本洗衣机前通读所有说明。 • 请遵照本安装说明安装洗衣机。请参考安装手册中 有关
LC480Software1.5中文使用说明(简单版)
LightCycler480 Software 1.5软件操作（中文版）第一部分基本界面及图标概述：一、Overview界面Exit：关闭LC480软件Log off：从目前使用的数据库中退出并可登陆其他的数据库Overview：点击该图标进入“Overview”界面Navigator：点击该图标进入“Navigator”界面，可进行数据的导入导出等操作，详见。
Save：点击该图标进行保存Export：导出当前打开的文件Close：关闭当前打开的文件Print：打印当前打开的窗口Tools：打开“Tools”界面，可进行密码修改，建立和编辑用户，系统设置，查看数据库状态、仪器状态、滤光片组合等操作Help：查看软件版本，操作说明书等二、New Experiment 界面Run Module：编辑、运行或查看PCR程序及查看PCR实时数据Subset Editor：点击该模块后可进行子集的编辑Sample Editor：点击后进行样品信息的编辑Analysis Module: 点击进行结果分析或查看已保存的分析结果Report Module：选择需要的内容以报告的形式给出结果Summary Module : 查看实验的总体概况，包括实验名称，所用的程序，检测模式，滤光片组合等。
概况的内容由系统自动生成三、Navigator 界面Import：导入一个文件Export：导出一个文件Batch Import：导入一批数据Batch Export：导出一批数据New：新建一个实验或文件夹New Folder：新建一个文件夹Open：打开一个文件Rename：重命名Delete：删除选中的目标Copy：拷贝选中的目标Note：所有上述图标在深蓝色时为激活状态，灰色时为灭活状态也就是不可用状态。
各图标的功能在不同状态下以颜色来区分是否具备。
软件操作一．打开软件打开电脑，Windows操作系统的用户名为operator，密码为LC480（区分大小写），电脑操作系统启动完毕，检查电脑右下角是否有图标。
lightcycler 480 Software1.5 中文说明书
LightCycler480 Software 1.5软件操作（中文版）第一部分基本界面及图标概述：一、Overview界面Exit：关闭LC480软件Log off：从目前使用的数据库中退出并可登陆其他的数据库Overview：点击该图标进入“Overview”界面Navigator：点击该图标进入“Navigator”界面，可进行数据的导入导出等操作，详见。
Save：点击该图标进行保存Export：导出当前打开的文件Close：关闭当前打开的文件Print：打印当前打开的窗口Tools：打开“Tools”界面，可进行密码修改，建立和编辑用户，系统设置，查看数据库状态、仪器状态、滤光片组合等操作Help：查看软件版本，操作说明书等二、New Experiment 界面Run Module：编辑、运行或查看PCR程序及查看PCR实时数据Subset Editor：点击该模块后可进行子集的编辑Sample Editor：点击后进行样品信息的编辑Analysis Module: 点击进行结果分析或查看已保存的分析结果Report Module：选择需要的内容以报告的形式给出结果Summary Module : 查看实验的总体概况，包括实验名称，所用的程序，检测模式，滤光片组合等。
概况的内容由系统自动生成三、Navigator 界面Import：导入一个文件Export：导出一个文件Batch Import：导入一批数据Batch Export：导出一批数据New：新建一个实验或文件夹New Folder：新建一个文件夹Open：打开一个文件Rename：重命名Delete：删除选中的目标Copy：拷贝选中的目标Note：所有上述图标在深蓝色时为激活状态，灰色时为灭活状态也就是不可用状态。
各图标的功能在不同状态下以颜色来区分是否具备。
软件操作一．打开软件打开电脑，Windows操作系统的用户名为operator，密码为LC480（区分大小写），电脑操作系统启动完毕，检查电脑右下角是否有图标。
Pacific Laser Systems PLS 480 产品说明书
•ON/OFF MENU SWITCH•BA TTERY DOOR •1/4 - 20 INSERTIN BASE OF EACH UNIT •POUCH •FLOOR TARGET• STANDARD FLOOR BASE•UNIVERSAL ADAPTER OPTIONAL• PLS SLD LASER DETECTOROPTIONALPlease review the PLS-SLD Laser Detector operating manual for use with PLS 480 for exterior application.O PERA TING M ANUALFEA TURES PLS 480P A C I F I C L A S E RS Y S T E M SThe Professional StandardMENU SWITCHUNIVERSALBASE-TRIPOD ADAPTERGREEN LIGHT:ONRED LIGHT:EXCEEDS TILT AMBER LIGHT:BA TTERY LOW SOLID LIGHT:INTERIOR BLINKING LIGHT:EXTERIORON/OFF / SQUARE LINES AND DOWN POINT ON/OFF / LEVEL LINESFIRST PRESS:SELF LEVELING MODE SECOND PRESS:OVER RIDE SELFLEVELING MODETHIRD PRESS:OFFPULSE:OUTDOOR MODE TO USE WITH SLDDETECTOR - SQUARE AND LEVELThe universal tripod adapter is included with the PLS 480 SYSTEM.Theadapter has two components.1) Floor and Batter Board Base and 2) T ripod -Fine T une Base.The two components can be used separately or together depending on the job site application.When using your PLS480 on the floor or concrete slab, attach the PLS480 to the combined adapter (top and bottom components together) by inserting its 1/4 - 20 bolt into the 1/4 - 20 insert on bottom of the PLS480.The reversible front foot can be turned around by loosening the screw-bolt and rotating to allow a straight edge against bottom track.The fine tune adjustment knob allows the PLS480 (or other PLS laser) vertical lines to move incremen-tally right or left.The top component also swivels right and left for maximum utility of the laser.When using your PLS480 onbatter board attach the PLS480 to the combined adapter (top and bottom components) and place the PLS480 and adapter on to the batter board.Secure the PLS480 to the batter board with the threaded clamp on back of the adapter.The down beam of the PLS480 should project closely in front of the batter e the adapter adjustable knobs to turn the square beams of the PLS480 (or other PLS laser) right or left to meet the target or SLD Detector.UNIVERSALBASE-TRIPOD ADAPTERUNIVERSALBASE-TRIPOD ADAPTERShown with optional universal base tripod adapter• FINE TUNING KNOBTWIST OFF/ON BASE• REVERSIBLE FOOTWhen using your PLS480 on a tripod secure the top component of the adapter to the tripod stud.Secure the PLS480 to the adapter and posi-tion the down point of the laser to point through the tripod handle or cantilever the tool so the down point can be seen between the tripod legs.Y ou can now adjust the laser for square layout using the down point as your spring point and capturing thesquare laser lines with your SLD Detector.SAFETY LABELINGCHECK SQUARECHECKING THE PLS480 ON THE JOB SITE USING PYTHAGORAS 3-4-5 TRIANGLE:Set up over the center of PEG (A) using the plumb down laser dot.Using one of the 90 degree beams put PEG (B)in the ground at an exact distance of 15M/45 FT from PEG (A).Using the other 90 degree beam, put PEG (C) in the ground at an exact distance of 20m/60 FT from PEG (A).Now measure with a tape diagonally from PEG (B) to PEG (C).The distance should be 25M/75FT .PEG A15M/45FT20M/60FT25M/75FTPEG BPEG C390o45CAUTION:USE OF CONTROLS, ADJUST-MENTS OR PROCEDURES OTHER THAN THOSE SPECIFIED HEREIN MAY RESULT IN HAZARDOUS RADIA TION EXPOSURE.PLS480 complies with US FDA standards, 21 CFR, Subchapter J.These labels are attached toevery PLS laser.These are not to be removed or defaced.PLS • 2550 KERNER BLVD., SAN RAFAEL, CA 94901800 601 4500AVOID EXPOSURE LASER RADIA TION EMITTED FROM THIS APERTUREPLS90E COMPLIES WITH FDA PERFORMANCE STDS.21 CFR, SUBCHAPTER JSERIAL NUMBER:MFG’D:CAUTION: LASER RADIATION WHEN OPEN. DO NOTSTARE INTO BEAM OR VIEW DIRECTLY WITH OPTICAL INSTRUMENTS. PATENT PENDINGREG. US PATENT OFFICEPLS 2656 BRIDGEWAY, SAUSALITO, CA 94965C A U T I O NLASER RADIA TIONDO NOT STARE INTO BEAM OR VIEW DIRECTL Y WITH OPTICAL INSTRUMENTSMAX OUTPUT POWER <1mWWAVELENGTH 635-670nm CLASS II LASER PRODUCTWARNING LABELBACKFRONTAPERTURE LABELID# CERTIFICATION LABELCHECK LEVELCHECK PLUMBSPECIFICA TIONSW ARRANTYLight source:Semiconductor laser diode 635-670 nm, visibleWorking range:+/- 200 feet (outdoor w/detector)Accuracy:Square and Level +/-1/8”@ 60 Feet(3mm@18 meters)Plumb Down +/-1/8”@ 20 Feet(3mm@ 6 meters)Leveling:Automatic Leveling range:+/- 6oPower supply:3AA batteries alkaline Operating time:+25 hours continuous use Operating temp:0o F to 122o F (-18o C to 50o C)Storage temp:-40 F to 158o F (-40o C to 70o C)Indicator:Green light:ONRed light:EXCEEDS TILT/Amber light:BA TTERY LOW Flashing:pulse beam- exteriorEnvironment:Water resistant;not submersible Dimensions: 5.6X4.6X2.9 inches Weight:1.25 lbs.(including batteries)This product is warranted by PLS·Pacific LaserSystems to the original purchaser to be free from defects in material and workmanship under normal use for a peri-od of three years (USA only) from the date of purchase.During the warranty period, and upon proof of purchase,the product will be repaired or replaced (with the same or similar model at our option), without charge for either parts or labor, through PLS.The purchaser shall bear all shipping, packing and insurance costs.Upon completion of the repair or replacement, the unit will be returned to the customer, freight prepaid.The warranty will not apply to this product if it has been misused, abused or altered.Without limiting the foregoing, battery leakage, dents or gouges to the plastic housing, broken optic windows,damage to the switch/LED membrane are presumed to result from misuse or abuse.T ampering with or removal of the caution or certification labels voids this warranty.Neither this warranty nor any other warranty, express or implied, including implied warranties of merchantability,shall extend beyond the warranty period.No responsibil-ity is assumed for any incidental or consequential dam-ages.This warranty gives you specific legal rights, and you may have other rights which vary from state to state.PLS Pacific Laser Systems2550 Kerner Blvd., San Rafael, CA 949011 800 601 4500 TO CHECK LEVEL:Choose two interior walls approximately 15’-0”apart (see Fig.1).Y ou can use 2 x 4s for targets.T urn on the PLS480 to Level setting #1 (level).With the PLS480 six inches away from and facing target #1, carefully mark the center of the bel this mark A1.Swivel the laser 180 degrees and mark the center of the line on target #bel this mark B1.Move the PLS480 six inches away from and facing target #2.Carefully mark the center of the bel this mark B2.Swivel the laser 180degrees and mark the center of the line on target #bel this mark A2.Y ou now have two centers of elevation at each target.Carefully measure the distance between centers of each set of marks.If there is a differ-ence, subtract one measurement from the other.This calibration method magnifies any error by a factor of two.Therefore divide any difference by two to find the true error.Y our PLS480 should have an error of no greater than 1/8in.within 60ft.Target #1Target #2A1+ A2B1+ B2-15’Place the PLS480 on the standard floor base and position the PLS480 so that the down point is visible on the floor.Mark the down point position and without moving the laser also mark the intersecting point at which the two vertical lines meet on the ceiling.Turn the laser 180 degrees and place the down point on the original mark on the floor.The intersecting point on the ceiling should match the original ceiling mark.If there is a difference between the original ceiling mark and second mark,the error of the plumb beam is half that difference.We recommend that you check your PLS480periodically in order to assure its accuracy.。
480 操作手册 -1
1. 本機總重量約為 3 公噸，所有搬運設備之安全承載負重均須大於 3 公噸以上，方可使用，如 果無法確定搬運承載設備之安全承載荷重時，應該詢問搬運承載設備之製造商，並且請求確 認，切勿於不了解狀況之下自行嘗試搬運，否則可能造成搬運設備或機器損毀，甚至會造成人 員傷亡。
2. 如使用起重機吊掛時，為避免機器受損則必須使用本機特殊設計之專用吊架，切勿自行以不合 法之吊具以及不對的方式吊掛，否則將會造成搬運設備或機器損毀，甚至造成人員傷亡。
看指示銘牌或參考維護手冊所述之標準。 5. 回裝零件時必須確實嚴謹的檢查操作安全護蓋及安全裝置是否適當。
暖機:
當機器長時間閒置之後不經過暖機過程，就馬上加工零件時，會將滑動件燒壞引發漏油現象， 而且也會因機器突然間受到熱澎脹影響導致破壞機器的精度，為了預防此現象，一般新機器和長 期閒置之機器在做加工前必須要做暖機動作。
刀具干涉，否則容易引起意外傷害，刀具長度及外徑之設定請參考操作手冊。 4. 刀具安裝後要必須試車削，以確認刀具無任何問題。
操作:
警告項目:
1. 切勿戴手套操作開關，會引起故障危險。 2. 操作人員勿留長髮以避免發生不必要的危險。 3. 繩索、吊具或吊物機械，必須有足夠的強度以承受負載。 4. 使用堆高機、起重機或類似設備必須特別小心，當每次操作時需預防碰撞和損壞周圍環境。 5. 堆高機、起重機、吊車及其它類似設備的操作，只有受過訓練的操作員才能勝任。 6. 移動較大工件時，需兩人或兩人以上一起搬動，以免發生危險。 7. 加工切削時必須安全牢靠的夾持工件。 8. 應先停止機械後才能調整切削液噴嘴至刀尖處。 9. 切勿用手去觸摸轉動中工件或主軸....等旋轉中之機器零件。 10. 不要任意除去機器的安全護蓋。 11. 為避免割傷危險，不要直接用手去除刀具上的鐵屑，而必須使用刷子去除。 12. 必須停止機器運轉才可以裝置、拆卸刀具。 13. 嚴格禁止人員在未俱有保護裝備下於粉塵環境中工作，當機械在切削石墨工件或有粉狀切屑
480 操作手册-2
5. 手动换刀被放松并推出。一手
压着开关另一手将要锁上的刀具对准主轴刀上的定位键(drive key)，刀具插上后，放开手动
退刀开关，刀具即被锁紧在主轴上。
注意：为保持主轴的精度，将刀具锁上主轴之前请先将主轴刀柄座斜度部分及将锁上的刀柄 擦拭清洁再锁上。
2.4 机器的开机及关机
冲屑开关 押此按钮开关启动冲屑用切削水;再押一次停止冲屑切削水。
切削水程序连锁开关 切削水之由程序来指令控制。
工作灯 手动控制工作灯开关。
蜂鸣器解除 警报产生时，蜂鸣器会响，操作者可按此开关先将蜂鸣器暂时解除，再做问题排除的工作。
紧急停止解除 2-5
当 X/Y/Z 任何一轴有超过行程，EM 指示灯亮起。持续押此按钮开关并使用微调模式将进入紧急停 止开关内之轴反方向移开 100mm 时再放开，紧急停止区域的情况即可解除。
第4轴 手动移动第 4 轴，可依第 4 轴按钮开关上之方向标示¸移动第 4 轴之方向。轴回到机械原点时，灯会亮。
各类按钮开关功能说明
M01 (选择性停止) 注意!! 此操作开关依操作者之需要作选择，当开关的指示灯亮时表示 M01 机能有效。 在 M01 机能有效情况下： 1. M01 单节背执行。 2. 各轴与主轴会停止。(主轴不停止可由 DGN 设定，参考电器手册) 3. 切屑水停止与进给暂停指示灯亮。(切屑水不停止可由 DGN 设定，参考电器手册) 4. 押循环启动按钮开关后，程序继续执行。 5. 进给暂停指示灯熄灭。
手动原点复归
1. 选择
模式。
2. 押下欲回原点的轴"＋或－"方向的按钮，轴即会往"＋/－"方向移动。当该轴原点复归动作完 成时，原点位置表示灯会亮起。
原点复归注意事项
LC480软件1
LC480软件1概述本文档旨在介绍LC480软件1.5版本的中文功能。
LC480软件是一款用于分析和解释实时定量PCR结果的强大工具。
功能特点LC480软件1.5中文版本拥有以下主要功能特点：数据处理- 数据导入：支持从LC480仪器或其他PCR仪器导入实验数据。
数据导入：支持从LC480仪器或其他PCR仪器导入实验数据。
- 数据分析：可对数据进行统计分析，包括周期数(Cq)、标准曲线以及PCR效率。
数据分析：可对数据进行统计分析，包括周期数(Cq)、标准曲线以及PCR效率。
- PCRD-PCR反应设计：提供简便快捷的PCR反应设计工具，帮助用户优化PCR反应条件。
PCRD-PCR反应设计：提供简便快捷的PCR反应设计工具，帮助用户优化PCR反应条件。
结果解释- 结果可视化：以图表形式展示PCR扩增曲线、熔解曲线和标准曲线，帮助用户直观地解释实验结果。
结果可视化：以图表形式展示PCR扩增曲线、熔解曲线和标准曲线，帮助用户直观地解释实验结果。
- 结果导出：支持将结果导出为图像文件或数据文件，方便用户进行进一步的数据处理和分析。
结果导出：支持将结果导出为图像文件或数据文件，方便用户进行进一步的数据处理和分析。
数据管理- 项目管理：支持创建、编辑和删除项目，方便用户对实验数据进行组织和管理。
项目管理：支持创建、编辑和删除项目，方便用户对实验数据进行组织和管理。
- 数据备份与恢复：提供数据备份和恢复功能，保障数据的安全性和可靠性。
数据备份与恢复：提供数据备份和恢复功能，保障数据的安全性和可靠性。
多样性分析- 样本分组：支持针对实验样本进行分组分析，方便比较和对照分析。
样本分组：支持针对实验样本进行分组分析，方便比较和对照分析。
- 丰度分析：提供注释和分类功能，帮助用户对实验样本的物种丰度进行分析。
丰度分析：提供注释和分类功能，帮助用户对实验样本的物种丰度进行分析。
导入和导出- 模板导入：支持导入模板文件，减少手动输入的工作量。
LC480操作与维护规程
文件类型技术文件机密等级内部公开文件编号W-SBYQ-***工艺代号/Roche LightCycler 480Ⅱ实时荧光定量PCR系统操作与维护规程参考资料文件编号说明文件发放部门(必填)保密文件，知识产权属迈瑞公司所有。
第1 页共19 页(3.0)保密文件，知识产权属迈瑞公司所有。
第 1 页 共 19 页修订记录版本 ECN/CR/PCN/TC N修 改 内 容 概 述修订人 生效日期(注：此模板3.0版开始加入“生效时间”列，故之前修订记录中此项信息为空)Roche LightCycler 480Ⅱ实时荧光定量PCR系统操作维护规程1目的使Roche LightCycler 480Ⅱ实时荧光定量PCR系统能够正确和正常的使用，保证系统的正常运行的准确性和精密度，以获得准确可靠的检验结果。
2适用范围使用Roche LightCycler 480Ⅱ实时荧光定量PCR系统进行相应的临床项目检测，并经过系统维护保养工作培训（至少已经阅读过系统操作手册和保养手册）的试剂研发工程师和厂家维修工程师。
3术语和定义3.1实时荧光定量PCR技术实时荧光定量PCR技术指在PCR反应体系中加入荧光基团，利用荧光信号积累实时监测整个PCR进程，最后通过标准曲线对未知模板进行定量分析的方法。
实时荧光定量PCR技术于1996年产生，由于该技术不仅实现了PCR从定性到定量的飞跃，而且与常规PCR相比，它具有特异性更强、有效解决PCR污染问题、自动化程度高等特点，目前已得到广泛应用。
3.2实时荧光定量PCR系统实时荧光定量pcr仪，由荧光定量系统和计算机组成，用来监测循环过程的荧光。
与实时设备相连的计算机收集荧光数据。
数据通过开发的实时分析软件以图表的形式显示。
原始数据被绘制成荧光强度相对于循环数的图表。
原始数据收集后可以开始分析。
实时设备的软件能使收集到的数据进行正常化处理来弥补背景荧光的差异。
正常化后可以设定域值水平，这就是分析荧光数据的水平。
福立冰机操作和维护手册说明书
HCD700R/N, HMD700R/NIce MachinesOperation and Service Manual Serial Numbers After H58642Order parts online801 Church Lane • Easton, P A 18040, USA T oll free (877) 612-50861 • +1 (610) 01012715R07Table of contentsWelcome to Follett Corporation (4)Specifications (5)Operation (7)Cleaning, weekly exterior care (7)Cleaning, semi-annual evaporator cleaning (7)Service (12)Ice machine operation (12)Water system (13)Electrical system (14)Normal control board operation (14)T est points (15)Error faults (15)Hard errors (15)Soft errors (15)Relay output indication (15)Compressor/refrigerant solenoid output (15)Wiring diagram, evaporator unit (16)Wiring diagram, condenser unit (17)Gearmotor data (17)Mechanical system (18)Evaporator disassembly (18)Evaporator reassembly (20)Refrigeration system (23)Condenser unit operation (23)Refrigerant pressure data (23)Refrigerant charges (23)Refrigeration system diagram (24)Refrigerant replacement requirements (25)Evacuation (25)Ambients (evaporator unit) (25)Ice capacity test (25)Bin full detection system (26)T roubleshooting (27)Replacement parts (30)Fig. 1Fig. 2 2. Mix 1 gallon (3.8L) 120 F (49 C) water and7 ounces (198g) (one 7 ounce packet of FollettSafeCLEAN ice machine cleaner, part# 00132001).Locate cleaning cup. Fill until CLEANER FULL lightcomes on (Fig. 2).Note: Do not use bleach to sanitize or clean the ice machine.CLEANERFULL Fig. 33. Replace cover on cleaning cup. Wait until machinerestarts. Machine will clean, then flush 3 times inapproximately 15 minutes (Fig. 3).4. T o sanitize – Press CLEAN button. The machine willdrain. Wait for LO WA TER light to come on(Fig. 4).Fig. 5 5. Mix 1 gallon 120 F (49 C) water and 1.6 ounces (48ml)NU-CALGON IMS-II or IMS-III SANITIZER. Fill untilCLEANER FULL light comes on (Fig. 5).Place one Sani-Sponge™ in remaining sanitizing solutionand retain for Step 9.Note: Do not use bleach to sanitize or clean the ice machine.CLEANERFULL Fig. 6Fig. 88. Using disposable food service grade gloves, insert drySani-Sponge™ (kit part# 00132068). Next, insert Sani-Sponge soaked in Nu-Calgon IMS-II or IMS-III sanitizer solution (from Step 5). Push both Sani-Sponges down ice transport tube with supplied pusher tube (Fig. 8).Fig. 99. Remove and discard 16" (407mm) pusher tube (Fig. 9).Fig. 1010. Reconnect coupling. When sanitizing cycle ends,machine will start producing ice. Press power switch ON.Ice pushes Sani-Sponges through tube (Fig. 10).11. Place a sanitary (2 gallon or larger) container in bin ordispenser to collect Sani-Sponges and ice for 10 minutes.Collect 5.5 lbs (3kg) of ice from unit. Discard ice andSani-Sponges (Fig. 11).ServiceIce machine operation (all models)Follett’s ice machine consists of five distinct functional systems covered in detail as follows:§Water system§Electrical control system §Mechanical assembly §Refrigeration system§Bin fullThe Horizon ice machine overviewThe Follett Horizon ice machine uses a horizontal, cylindrical evaporator to freeze water on its inner surface. The refrigeration cycle is continuous; there is no batch cycle. The evaporator is flooded with water and the level iscontrolled by sensors in a reservoir. A rotating auger (13 RPM) continuously scrapes ice from the inner wall of the evaporator. The auger moves harvested ice through the evaporator into an ice extrusion canal. The ice is forcedthrough a restrictive nozzle that squeezes out the water and creates the Chewblet. The continuous extrusion process pushes the Chewblets through a transport tube into a dispenser or bin.A solid state PC board controls and monitors the functionality of the ice machine. In addition to sequencing electrical components, the board monitors various operational parameters. A full complement of indicator lights allows visual status of the machine's operation. Additionally, the PC board controls the self-flushing feature of the ice machine. The evaporator water is periodically drained and replenished to remove minerals and sediment.A unique “bin full” detection system is incorporated in the Horizon ice machine. A switch located at the ice discharge port of the machine detects the position of the transport tube. When the bin fills up with ice, the transport tubemoves out of the normal running position, and the switch turns the ice maker off. A domed housing at the end of thetransport tube contains the ice extrusion loads during shut down.Harvest system diagramDIP Switch SettingsError faults:The Horizon PC board monitors various operating parameters including high pressure, auger gearmotor amperage limits, clogged drain, and low water alarm conditions. There are two types of errors namely “hard” or “soft”. A hard error is one that shuts the machine off and will not allow restart until the reset button is pressed. Even cycling power will not reset a hard error. A soft error can either be automatically reset should the condition rectify, or if power is cycled. Should an error occur, consult the troubleshooting guide in this manual or a Follett service technician.Soft errors:HI AMPS: The PC board monitors the amperage of the auger motor. Should the gear motor experience current draw above the allowable limit, the machine will shut down and the TIME DELAY and HI AMP will be illuminated. After the time delay the machine will restart and the TIME DELAY and HI AMP will clear.LO WA TER: During operation, the water level cycles between the normal low and normal high sensors. Should the water be shut off to a running machine, a soft error will occur. The error sequence is as follows: During operation,the water level falls to the normal low sensor, and when it does the water feed solenoid is energized. If water is not detected at the normal low sensor within 10 seconds, a soft error will occur. The machine will shut down, but the water feed solenoid will remain energized. Should water return, it will fill to the normal low sensor and the machine will resume normal operation. The error will clear automatically.HI PRESSURE: Should the refrigeration pressure rise above 425 psi, the machine will shut down and the TIME DELAY and HIGH PRESSURE will be illuminated. After the time delay, and if the pressure has fallen back below the reset point of 295 psi, the machine will restart and the TIME DELAY and HIGH PRESSURE will clear.Hard error:DRAIN CLOG: The drain clog sensor, located in the chassis, underneath the rear drain pan, will detect the presence of water just below the top edge of the pan. If water does not properly flow out of the drain pan it will overflow into the chassis and rise to the sensor (especially during a self-flushing purge cycle). Pressing the reset button will restart the ice machine.Relay output indication:Each relay on the board has an indicator light associated with its output. For example, when the relay for the water feed solenoid is energized, the adjacent indicator light glows green.Comp/Sol output:The output for the liquid line solenoid valve is labeled COMP/SOL.Flushing logicFlush on fly: For every one (1) hour of ice making time, the machine will open the drain valve for a duration of 60 seconds. While the drain valve is open, the machine will continue to make ice and the water feed valve will cycle to maintain water level.Off cycle: At the completion of off-cycle time delay, the machine checks for a cumulative one (1) hour of ice making time since the last off-cycle flush. If the cumulative ice making time exceeds one (1) hour, the machine will open the drain valve for 60 seconds to drain the evaporator in its entirety. It will then refill with water and begin making ice. If the ice making time is less than 1 hour, the machine will start and begin making ice without draining the evaporator.Mechanical System Fig. 125. Remove gear motor:§Remove gear motor insulation (Fig. 15.1).§Remove 1/4-20 screw, retainer, and spacer (Fig. 15.2).§Remove two 1/2" bolts (Fig. 15.3).§Pull gear motor from auger (Fig. 15.4).§Remove main housing insulation (Fig. 15.5).6. Remove all traces of petro-gel from auger shaft.7. Remove main housing:§Use an allen wrench to remove 3/16" allen screws (3) (Fig. 16.1).§Remove shaft insulation (Fig. 16.2).§Remove main housing (Fig. 16.3).8. Remove and discard mating ring and seal (Fig. 17.1).9. Carefully remove auger (Fig. 17.2).ARNING!very sharp - handle with care to avoid personalinjury.10. Press the lever on the back of the reservoir(Fig. 18.1) to release and remove the solenoid (Fig. 18.2).11. Remove three screws to remove the reservoirinsulation (Fig. 19).12. Remove three screws to remove the reservoir(Fig. 20).13. T o remove the rear bushing, place the auger into theevaporator and use it to gently tap and dislodge the rear bushing housing (Fig. 21).Evaporator reassembly1. Remove and inspect O ring seal. Replace ifdamaged in any way.2. Place rear bushing into evaporator.3. Install the three bolts: partially tighten each bolt,alternating bolts until the rear bushing is fully seated and properly aligned (Fig. 22).4. When fully seated, remove the bolts.5. Install the reservoir with three bolts (Fig. 23).6. Install the reservoir insulation, tube clamp, andsolenoid lever with three screws.7. Install solenoid.1. Remove and inspect O ring seal. Replace ifdamaged in any way (Fig. 25.1).2.and reinstall (Fig. 25.2).3. Use cardboard disc to press new mating ring intomain housing (Fig. 26.1).4. Lube the shaft with liquid soap in the area shown(Fig. 26.2) and slip on seal and spring (Fig. 26.3).Note:hands. Contact with bare skin will causepremature seal failure.5. Install auger (Fig. 26.4).6. Install main housing:§Slide main housing onto auger shaft (Fig. 27§Install main housing insulation (Fig. 27.2).§(Fig. 27.3).7. Apply a coat of petrol-gel to the auger shaft.8. Install gear motor:§Install main housing insulation (Fig. 28.1).§Slide gear motor onto the auger shaft§Install two 1/2" bolts (Fig. 28.3).9.motor shaft keyway (Fig. 29.1).10. Install key into keyway (Fig. 29.2).11.(Fig. 30.1)12. Insert screwdriver into groover of auger shaft and pryshaft outwards (Fig. 31.1).13. Insert retainer into groove (Fig. 31.2), ensure thatretainer is aligned with hole in spacer.14. Install screw and tighten (Fig. 32.1).15. Install gear motor insulation.Fig. 34 16. Install shuttle housing: Array§Install stream divider (Fig. 34.1).§Place shuttle housing and install two screws (Fig. 34.2).§Plug in shuttle housing connections (Fig. 34.3).§Connect vent tube (Fig. 34.4).17. Plug in gear motor.§BROWN to BLUE§BLACK to BLACK§WHITE to GRAY18. Connect transport tube to louvered dockingassembly.Refrigeration system diagram“Bin full” detection systemThe Follett Horizon ice machine incorporates a unique “bin full” detection system that consists of the shuttle and actuator. The shuttle incorporates a flag and switch. Referencing the figure below, the normal running position of the flag is down, and the switch is closed. When the bin fills to the top and ice can no longer move through the tube, the machine will force the shuttle flag up, opening the switch and shutting the machine off. The shuttle actuator, located above the ice bin allows the ice to curl up within it when the bin is full. In this way, there are no loads generated that would tend to lift off the lid of the bin.TroubleshootingPlease see “Service” section for a description of each function.ATTENTION!T o prevent circuit breaker overload, wait 15 minutes before restarting this unit. This allows the compressor to equalize and the evaporatorto thaw.Order parts online Order parts online Electrical boxOrder parts online Order parts online Skins assembly Order parts onlineOrder parts online Order parts online Harmony, Ice Manager, SafeCLEAN, Sani-Sponge and Vision are trademarks of Follett Corporation Follett, Chewblet, and RIDE are registered trademarks of Follett Corporation, registered in the US.。
Roche LightCycler 480 中文操作说明要点
LightCycler® 480 中文操作说明罗氏医学仪器公司 / 应用科学部门February 2008目录一. 启动机器与软件 3二. 软件设定 --- 开启新实验 5三. 样品编辑9四. 结果分析12五. 报告18六. 数据转移至其它计算机(数据输出与输19 入)七. 使用者管理19一．启动机器与软件•启动机器1.机器主开关位于机器右后方。
2.机器正面两个警示灯代表机器状态。
待警示灯 (右) 由闪烁橘色灯转变成稳定橘色灯，警示灯 (左) 由橘色转变成绿色 (约三分半钟)，表示机器已经启动完成。
3.按压机器正面上唯一的按钮，放入已经封膜完成之 96 或 384 孔盘。
放置完成后，左右两警示灯都呈现绿色。
•启动软件1.开启计算机，并以正确的使用者名称与密码登入 Windows。
User name: OPERATORPassword: LC4802.开启 LightCycler 480 软件，并输入正确的使用者与密码。
二．软件设定-- 开启新实验•设定新实验1.软件开启后，会进入主画面。
点选“New Experiment”。
2.出现实验设定窗口。
任何时候需要回主画面的话，点选画面右边的按钮。
•设定实验❖若要套用设定好的实验步骤 (Template)，请跳至 6.。
1.在实验设定画面上方，先选择适当的 Detection Forma t。
选项包含:另外可再点选“Customize” 勾选所需的滤片组合。
2.输入反应体积: 96 well plate 范围为 10-100 ul，而 384 well plate 范围为 5-20 ul。
3.设定实验步骤 (Program)•设定 program，包含有 Pre-incubation, Amplification (PCR), Melting curve (optional) 与 Cooling。
利用『+』与『－』增加或删除步骤。
•设定循环数目 (Cycle) 与分析模式。
LightCycler480日常维护和保养
Exor4 数据库
右击鼠标
选择 Backup Database
LightCycler® 480数据库备份
选择D:\ 保存路径备份数据库（建议日常保存5个文件）
自动以操作日期存档
LightCycler® 480单次实验数据备份
LightCycler® 480单次实验数据备份
LightCycler® 480单次实验数据备份
• 实验室配备有良好的接地线
LightCycler® 480：96孔模块与384孔模块互换
• 可互换的热循环模块 • 自己动手DIY，快速方便 • 随机附送装载箱，便于更 换模块 • 无需厂家工程师在场 • 无需重新校准 • 更换完毕后仪器自动检测 并确认模块型号
LightCycler® 480数据库备份
LightCycler® 480日常维护与保养 罗氏应用科学部
LightCycler® 480安装场地要求
• LC480 UPS≥2500 VA
• 实验室温度：10 ± 5℃ • 实验室湿度：20% - 80%
பைடு நூலகம்
• 实验室配备有空调做温度及湿度的调控
• 按照指定路径保存运行文件（包括所 有 反应程序和数据以及分析结果等） • 可以作为实验数据寻求技术支持
LightCycler® 480仪器清洁
• 仪器表面的日常清理（75%酒精擦拭表面）
• 防尘布或者防尘罩保护仪器 • 电气安全
We Innovate Healthcare
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LightCycler480荧光定量PCR仪操作规程1．每次反应最后一步都必须做降温，降至40度，维持至少30秒。
2．在操作过程中，切忌戴有粉末的乳胶手套接触光学透性封板膜。
3．试剂加完样，封膜完毕后，在板式离心机中1500×g（3000rpm）离心两分钟，以排除气泡。
4．先开电脑在开仪器，先关仪器再关电脑。
打开总电源，打开连接LightCycler480荧光定量PCR仪的电脑，并打开LightCycler480 Software软件，然后打开LightCycler480仪器后方的电源开关，此时仪器会在与电脑软件接通后进行自检，仪器正面右边两盏桔黄色的灯会闪烁。
自检完毕后，左边的灯会变绿，右边的灯仍为桔黄色。
这时按一下灯右边带双箭头的开关，仪器的载板器会自动伸出，将准备好的反应板放入载板器中（反应板的切角方向应与载板器的切角方向相应才能将反应板正确放入，切勿用裸手接触反应板的光学透性封板膜）。
再按一下带双箭头的开关，仪器的载板器会自动缩入仪器内，此时仪器能自动检测到反应板，原来呈桔黄色的灯会变绿，LightCycler480 Software中Start Run按钮也同时变为激活状态。
5．设定完实验程序并编辑完样本后，点击软件中Start Run按钮，运行实验。
6．软件界面上出现Run Complete….标识时，说明实验运行完毕，按一下仪器上带双箭头的按钮，使载板器伸出，取下反应板，再按一下带双箭头的按钮，使载板器缩回，关闭LightCycler480仪器后方的电源开关，在电脑上分析实验数据。
7．分析完实验数据后保存结果，打印报告，并关闭电脑，关闭总电源。
实验员在离开PCR扩增区时带走用完的反应板。
维护SOPLightCycler 480是一台免维护的仪器。
做任何维护工作前，须确认主机电源已被关闭。
预防性维护：1．检查LightCycler 480主机两侧与背后空间，确保无任何阻碍空气流通的物品，包括书本、纸张或其他任何物品；2．若主机表面和桌面有灰尘，用不起毛的软布蘸少量清水擦拭干净。
清洁性维护：若有需要，可用性质温和的商品化清洁剂或70％的乙醇溶液对LightCycler 480主机表面、热循环96孔模块和热盖进行清洁。
清洁96孔热循环模块：1. 用移液器移取125μl 70％乙醇溶液或异丙醇至所有孔内；2. 15分钟后，使用移液器对孔内溶液进行多次吹洗；3. 移除孔内的液体，并使96孔模块孔内自然风干；注意操作时不要让清洗液腐蚀热循环模块的涂层。
LightCycler480 Software 1.5软件操作（中文版）第一部分基本界面及图标概述：一、Overview界面Exit：关闭LC480软件Log off：从目前使用的数据库中退出并可登陆其他的数据库Overview：点击该图标进入“Overview”界面Navigator：点击该图标进入“Navigator”界面，可进行数据的导入导出等操作，详见。
Save：点击该图标进行保存Export：导出当前打开的文件Close：关闭当前打开的文件Print：打印当前打开的窗口Tools：打开“Tools”界面，可进行密码修改，建立和编辑用户，系统设置，查看数据库状态、仪器状态、滤光片组合等操作Help：查看软件版本，操作说明书等二、New Experiment 界面Run Module：编辑、运行或查看PCR程序及查看PCR实时数据Subset Editor：点击该模块后可进行子集的编辑Sample Editor：点击后进行样品信息的编辑Analysis Module: 点击进行结果分析或查看已保存的分析结果Report Module：选择需要的内容以报告的形式给出结果Summary Module : 查看实验的总体概况，包括实验名称，所用的程序，检测模式，滤光片组合等。
概况的内容由系统自动生成三、Navigator 界面Import：导入一个文件Export：导出一个文件Batch Import：导入一批数据Batch Export：导出一批数据New：新建一个实验或文件夹New Folder：新建一个文件夹Open：打开一个文件Rename：重命名Delete：删除选中的目标Copy：拷贝选中的目标Note：所有上述图标在深蓝色时为激活状态，灰色时为灭活状态也就是不可用状态。
各图标的功能在不同状态下以颜色来区分是否具备。
软件操作一．打开软件打开电脑，Windows操作系统的用户名为operator，密码为LC480（区分大小写），电脑操作系统启动完毕，检查电脑右下角是否有图标。
如果没有，点击桌面“Exor4 for XDMS_R”快捷方式文件。
然后右键点击图标，左键点击“Show”一栏，如最后一句话显示“Exor 4 server is running”表示数据库加载成功。
如果显示“Exor4 failed to start”则表示数据库没有加载上，关闭该窗口，右键点击图标，选“Shutdown”一栏，退出数据库，重新点击桌面“Exor4 for XDMS_T”文件，加载数据库。
然后点击桌面“LightCycler480 Software”快捷方式文件，运行该软件。
输入用户名和密码。
用户名默认为admin（管理者权限），密码为LightCycler480（区分大小写），如用户已经建立新用户名，则可以按照新用户名登录。
二．用户管理为了实验结果的有效管理，可以建立不同级别的用户。
如: 标准用户（Standard User）、专业用户（Expert User）、管理者（Local Administrator）。
一般实验室建议建立专业用户（Expert User）用户名，然后以该用户名登录软件进行实验操作。
建立新用户操作：打开LightCycler480 操作软件，点击右侧按钮打开“Tools”工具栏，选择“User Access/Users and Groups”选项。
在右边窗口中点击“New User”键，在右边“Enter the user’s full name”一栏中填写用户的全名，在“”Enter the name the user wants to log in as”一栏填写用户登录名，在“Enter the user’s password”一栏中输入密码（密码须含有6个以上字母，1个以上数字，其中有至少一个字母大写），在“Confirm the password”一栏中重新输入一次密码，加以确认，在“Select the user’s role”一栏中选择用户级别，然后点击右下方“Close”按钮加以确认。
在“Tools”工具栏中的“System Settings”界面中可以设置各用户级的权限，以及密码的时效。
三．仪器自检每次开机时，仪器会自动进行初始化自检程序。
自检通过，仪器左侧指示灯变绿。
如放置了样本，仪器右侧指示灯变绿。
在自检时，不能放置反应板，否则自检无法通过。
四．建立实验程序打开LightCycler480 操作软件，成功登录后点击按钮，进入New Experiment/Run Protocol程序设置界面。
在“Detection Format”一栏中选择所使用的荧光检测技术，可选择各类探针和染料，如常见的SYBR Green I染料、Mono Color Hydrolysis Probe单色TaqMan水解探针、Multi Color Hydrolysis Probe多色TaqMan水解探针。
在进行多色荧光PCR实验时，须点击按钮选择用户所采用的荧光检测通道，点击“√”确认。
在一栏中填写反应体积。
在“Programs”界面中设置实验程序：在“Program Name”下方命名程序，“Cycles”一栏内填写该程序的循环数，在“Analysis Mode”一栏选择该程序的分析模式。
定量分析选择“Quantification”，溶解曲线分析选择“Melting Curves”，颜色补偿实验选择“Color Compensation”。
而后在“Temperature Targets”界面内设置所指定程序的内容，在“Target(℃)”处填写目标温度，“Acquisition Mode”处选择信号采集方式，“Hold”一栏填写该温度所持续的时间，“Ramp Rate(℃/s)”处填写升降温速率（注：如目标温度为50℃以下时，为了减少硬件的损耗，必须将降温速率调至1.5℃/s以下）。
如一个程序有多个步骤，则在Temperature Targets界面的左侧处点击，增加步骤；也可点击上下箭头调整各步骤的先后顺序。
如一个实验中有多个程序，也可在“Programs”界面左侧点击，增加程序，也可点击上下箭头调整各程序的先后顺序。
实验程度设置完成后，在下方“Overview”界面内会自动生成温度随时间变化的温控曲线，以及估计的运行时间，绿色点表示采集荧光信号的温度和时间点。
可以依照该图检查一下程度设置是否正确。
设置好的实验程序可以保存成模板文件，下次使用同样的实验程序时可以调用。
具体操作如下：点击按钮右侧的下拉箭头，选中“Save As Template”选项，系统默认将实验程序模板文件保存在所登录的用户名下“Templates”目录中，用户可将其存放在其下的子目录“Run Templates”下，填写该程序模板文件的名称，点击“√”按钮即完成保存。
调用模板程序时可点击“Apply Template”按钮，从目录树中选定所要调用的模板，点击“√”按钮确认。
五．样本设置1、样本亚组编辑：LightCycler480软件拥有一个独特的样本编辑管理功能——亚组编辑（Subset Editor），即将反应板上所有的样本根据用户需要分成亚组，对亚组进行独立地分析定量。
具体操作如下：在“New Experiment”界面中，点击左侧按钮，进入Subset设置界面，点击按钮新建亚组，并给其命名。
点击按钮可复制所选定的亚组，点击按钮可更改所选定亚组的名称，点击“Delete”按钮可删除所选定的亚组。
设置亚组：在Subsets界面中选定要设置的亚组名，在右边反应板孔位处选定该亚组所包含的孔位，使所选定的孔位中都出现深兰色凹陷的，点击下方按钮加以确认。
设置亚组可以在一块反应板上实现多个检测项目的实验，前提是这些检测项目的实验运行程序都一致（这在使用TaqMan水解探针时比较容易实现），由于各检测项目均有各自的质控对照，因而，设定亚组可以单独对各检测项目进行分析。
2．样本属性编辑点击New Experiment界面左侧按钮设置样本信息。
在“Workflow”一栏在选择实验方案：Abs Quant：绝对定量或定性分析；Rel Quant：相对定量；Scanning：基因扫描；Color Comp：颜色补偿；Tm：熔解温度检测；Melt Geno：熔解曲线法基因分型；Endpt Geno：终点法基因分型。