抗纤软肝颗粒对四氯化碳加乙醇诱导肝纤维化大鼠的影响

抗纤软肝颗粒对四氯化碳加乙醇诱导肝纤维化大鼠的影响严骏松;周金辉;程良斌
【期刊名称】《中西医结合肝病杂志》
【年(卷),期】2017(27)1
【摘要】目的:观察中药抗纤软肝颗粒对四氯化碳(CCl4)加乙醇诱导肝纤维化大鼠的影响.方法:SD雄性大鼠40只,随机分为3组.分别为模型对照组(M)15只,抗纤软肝颗粒组(K)15只,正常对照组(N)10只.M、K组大鼠分别以40％CCl4橄榄油液腹腔注射,2 次/w,并以5％乙醇为唯一饮水;N组大鼠同期注射等剂量的橄榄油.造模同时,K组大鼠即予抗纤软肝颗粒2g/100g/d(中等剂量)灌胃,1 次/d;M、N组大鼠同期予等量生理盐水灌胃,共7周.观察大鼠肝脏病理学变化及血清总蛋白(TP)、白蛋白(Alb)、球蛋白(Glo)、Alb/Glo比值、ALT、AST的变化.结果:K组大鼠肝细胞变性坏死及肝纤维化程度较M组明显减轻,结缔组织明显减少,纤维间隔显著变薄,未见明显假小叶;M组大鼠血清Alb显著降低,ALT、AST均有明显升高(P＜0.05);K 组大鼠Alb回升,ALT及AST明显下降,与M组比较差异有显著性意义(P＜0.05).结论:抗纤软肝颗粒可通过保护肝细胞,降解纤维组织起到抗肝纤维化的作
用.%Objective:To observe the influence of Kangxian Ruangan
granules(KXRG) on hepatic fibrosis induced by carbon tetrachloride and ethanol.Methods:Fourty SD male rats were divide into 3 groups randomly:model control group (M) with 15 rats,KXRG group (K) with 15 rats and normal control group (N) with 10 rats.Group M and group K were injected with 40％ carbon tetrachloride olive oil at abdominal cavity twice per week.In addition,5％ ethyl alcohol was the only drinking water,group N
was injected with olive oil with same quantity at the same time.When modeling,group K was given by gavage once a day with 2g/100g/d (mild moderate dose) KXRG;group M and group N were given by gavage once a day with 2 g/100g/d normal saline (NS) for a total of 7 weeks.Observing the hepatic pathologic changes of experimental rats and the influence of the experiment on total protein,albumin,globulin,ALB/Glo ratio,ALT and AST.Results:hepatoeyte degenerative necrosis and fibrosis level in group K were lower than group M.In addition,connective tissues decreased obviously,fibrous septum became thinner significantly,there was no visible pseudolobule.ALB in group M decreased significantly,ALT and AST increased significantly (P ＜ 0.05).compared with group M,ALB in group K rised again,ALT and AST decreased significantly (P ＜
0.05).Conclusion:Kangxian Ruangan granule could prevent hepatic fibrosis by protecting the hepatocyte.
【总页数】3页(P40-41,49)
【作者】严骏松;周金辉;程良斌
【作者单位】湖北中医药大学2014级硕士研究生班,湖北武汉,430061;湖北中医药大学2014级硕士研究生班,湖北武汉,430061;湖北省中医院肝病研究所
【正文语种】中文
【相关文献】
1.抗纤软肝颗粒对肝纤维化大鼠肝脏MMP-1mRNA及TIMP-1mRNA表达的影响 [J], 胡泰洪;葛娅;张赤志;任星峰;朱清静;唐瑛;杨群;王晓昆
2.抗纤软肝颗粒对肝纤维化大鼠肝组织核因子-κB基因表达的影响 [J], 程良斌;程铭;刘艳
3.抗纤软肝颗粒对肝纤维化大鼠肝脏基质转换及肝窦毛细血管化的影响 [J], 胡泰洪;葛娅;张赤志;王晓昆;任星峰;唐瑛;朱清静
4.健脾软肝方对四氯化碳诱导肝纤维化大鼠肝窦基底膜形成的影响 [J], 陈文玲;杜良;陈文慧;谭丽萍;姚政;石安华
5.强力宁对四氯化碳、乙醇诱导的肝纤维化大鼠模型肝脏Ⅰ、Ⅲ型前胶原mRNA 表达的影响 [J], 郭津生;王吉耀;刘维田;余竹元
因版权原因，仅展示原文概要，查看原文内容请购买。