α-萘异硫氰酸酯诱导幼鼠亚急性肝内胆汁淤积的动态变化

α-萘异硫氰酸酯诱导幼鼠亚急性肝内胆汁淤积的动态变化欧巧群;钱新华;黄笑群
【期刊名称】《广州医药》
【年(卷),期】2014(000)005
【摘要】目的：制作亚急性肝内胆汁淤积动物模型，探索亚急性胆汁淤积发生的生化和病理变化。

方法幼年 SD 大鼠，分为对照组与 ANIT 模型组，分别给与芝麻油以及α-萘异硫氰酸酯（ANIT）灌胃，每周一次共4次，48小时后分批处死动物，取血标本送检肝功能指标，留取肝脏标本观察肝脏以及胆道病理改变。

结果ANIT 灌胃后48小时，血转氨酶、胆红素明显上升，肝脏出现结构坏死，炎症细胞浸润、增生，胆管增生，9天、16天逐渐加重，23天肝功能指标逐渐恢复，肝脏结构好转，胆管增生和纤维化仍明显。

结论成功制作幼鼠亚急性胆汁淤积的动物模型， ANIT 每周一次灌胃后2～3周内胆汁淤积明显，第4周生化和病理结构逐渐恢复，但胆管增生和肝纤维化仍然明显。

%Objective To create the sub-acute intrahepatic cholestasis model with infantile rats and explore the bio-chemical and pathological changes of sub-acute intrahepatic cholestasis. Methods Male SD infantile rats (1 50 ~1 80 g)were randomly divided into two groups.The ANIT (alpha-naphthyl-isothiocyanate)model group was gavaged with four weekly doses of ANIT (80mg/kg),the control group was gavaged with placebo sesame oil.Eight rats from the cholestasis group were sacrificed after 48h of the ANIT treatment every week.The blood samples were collected to assess AST,ALT,TBill,DBill,TBA,ALP and
γGT,and the liver tissue samples were made into paraffin sections and
observed with microscope. Results The level of serum
ALT,TBill,DBill,TBA,ALP and γGT was increased 48h after the ANIT treatment,and maintained at the high level un-til the third week.In the fourth week,the serum ALT,TBill,DBill and ALP declined quickly,however the serum TBA and γGT remained rising.Pathological changes were obvious 48h after the first ANIT treatment,characterizing with liver necrosis,inflam-matory infiltration,bile duct hyperplasia,which appeared gradually increasing until the third week.In the fourth week,the liver necrosis and inflammatory infiltration recovered normally,but the bile duct hyperplasia and liver fibrosis remained aggravating. Conclusion The sub-acute intrahepatic cholestasis model was created successfully with the ANIT gavaging weekly.The chole-stasis changes were the most obvious in the second and third week,but recovered in the fourth week except the bile duct hyper-plasia and liver fibrosis.
【总页数】4页(P7-10)
【作者】欧巧群;钱新华;黄笑群
【作者单位】广州市第一人民医院广州 510802;南方医科大学南方医院广州510515;广州市第一人民医院广州 510802
【正文语种】中文
【相关文献】
1.栀子柏皮汤对α-萘异硫氰酸酯诱导的肝内胆汁淤积大鼠的保护作用 [J], 曹璐;李俊;黄成;韩静文
2.麻黄连翘赤小豆汤对α-萘异硫氰酸酯诱导大鼠胆汁淤积性肝损伤的防护作用 [J], 刘翔; 廖雪梅; 张蓓
3.新型泛过氧化物酶体增殖物激活受体激动剂MBT1805对α-萘异硫氰酸酯诱导
的小鼠胆汁淤积症的治疗作用 [J], 王畅
4.茵陈术附汤抑制TLR4/NF-κB通路对α-萘异硫氰酸酯诱导肝内胆汁淤积症模型小鼠肝损伤的保护作用 [J], 王倩;苏慧宗;李玥;谭波;严东明;金静怡;裘福荣
5.甘草水煎液对α-萘异硫氰酸酯诱导的肝内胆汁淤积性损伤的防治及其机理研究[J], 沈淑娇;张志荣;张林林;樊玉娟;谭波;李玥;蒋健;裘福荣
因版权原因，仅展示原文概要，查看原文内容请购买。