SRPIN340_DataSheet_MedChemExpress

合集下载

ML324_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:ML324 is a potent JMJD2 demethylase inhibitor with demonstrated antiviral activity.IC50 value: 920 nM(JMJD2E) [1]Target: JMJD2 demethylase inhibitorML324 is a probe molecule that displays submicromolar inhibitory activity toward JMJD2E (in vitro) and possesses excellent in vitro ADME properties. In contrast to previously reported inhibitors of the JMJD proteins, ML324 displays excellent cell permeabilityproviding an opportunity for more extensive cell–based studies of JMJD2 enzymes to be undertaken. In addition, ML324 demonstrates potent anti–viral activity against both herpes simplex virus (HSV) and human cytomegalovirus (hCMV) infection via inhibition viral IE gene expression. ML324 suppresses the formation of HSV plaques, even at high MOI, and blocks HSV–1 reactivation in a mouse ganglia explant model of latently infected mice.PROTOCOL (Extracted from published papers and Only for reference)JMJD2E qHTS FDH Assay [1]:Enzyme and buffer solutions (3 μL) were dispensed into a 1,536–well Greiner black solid–bottom assay plate. The library compounds (23 nL) were transferred using a Kalypsys pintool equipped with 1,536–pin array. The plate was incubated at room temperature (15min), and then a 1 μL aliquot of substrate solution was added to initiate the reaction. The plate was transferred to ViewLux imager where an initial reading using standard UV optics (Ex 340 nm, Em 450 nm) was obtained. The plate was then removed from the reader,incubated for 30 minutes at room temperature, and returned to the reader for a second fluorescence reading. A fully automated robotic screening system (Kalypsys Inc, San Diego, CA) was used to perform the above steps as described previously. Compound plates containing DMSO as a vehicle–only control were included at regular interval throughout the screen to monitor any systematic trend in the assay signal associated with reagent dispenser variation or decreases in enzyme specific activity. For activity calculations,percent values were computed as the difference in fluorescence intensity between last and first time points. The percentage activity was calculated from the median values of the catalyzed, or neutral control, and the uncatalyzed, or 100% inhibited, control,respectively, using in–house software.Inhibition of viral infection in cell culture [1]:Cells were treated with DMSO, LSD1 inhibitor (TCP, tranylcypromine, Sigma P8511), or JMJD2 inhibitorsand infected with HSV–1 or hCMV as described below. cDNA was produced from total RNA and quantitated using an ABI 7900HT (ABI SDS 2.3 Software). Viral yields were determined by titration.References:Product Name:ML324Cat. No.:HY-12725CAS No.:1222800-79-4Molecular Formula:C 21H 23N 3O 2Molecular Weight:349.43Target:Histone Demethylase Pathway:Epigenetics Solubility:DMSO: ≥ 33 mg/mL[1]. Rai G, et al. Discovery of ML324, a JMJD2 demethylase inhibitor with demonstrated antiviral activity.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

HC-030031_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:HC–030031 is a potent and selective of TRPA1 inhibitor, which antagonizes AITC– and formalin–evoked calcium influx with IC 50s of6.2±0.2 and 5.3±0.2 μM, respectively.IC50 & Target: TRPA1[1]In Vitro: HC–030031 reversibly blocks TRPA1 currents with a similar potency, regardless of the agonist used; this includes blockade of currents elicited by reversible agonists, such as AITC, or irreversible agonists, such as N–methyl maleimide. HC–030031 blocks activation of TRPA1 by N–methyl maleimide, which opens the channel irreversibly through cysteine modification. HC–030031 does not block currents mediated by TRPV1, TRPV3, TRPV4, hERG, or NaV1.2 channels [1]. The potencies of HC–030031 versuscinnamaldehyde or allyl isothiocyanate (AITC or Mustard oil)–induced TRPA1 activation are 4.9±0.1 and 7.5±0.2 μM respectively (IC 50). These findings are similar to the previously reported IC 50 of 6.2 μM against AITC activation of TRPA1. The ability of HC–030031 to block TRPA1 activation is tested in a FLIPR calcium–influx assay using HEK–293 cells stably expressing human TRPA1. Concentrations of HC–030031 from 0.3 to 60 μM are incubated with cells for 10 minutes prior to addition of an EC 60 concentration of either cinnamaldehyde or AITC. HC–030031 dose–dependently blocks cinnamaldehyde– and AITC–induced calcium influx with IC 50 values of 4.9 and 7.5 μM, respectively [2].In Vivo: After injection of AITC (50 μL of 10%) into the rat hind paw, HC–030031 (300 mg/kg) significantly reduces flinching during the first 5 min. Over the remainder of the hour, HC–030031 decreases flinch frequency, a result that mirrors the effects observed on formalin–induced flinching [1]. In the rat, oral administration of HC–030031 reduces AITC–induced nocifensive behaviors at a dose of100 mg/kg. Moreover, oral HC–030031 (100 mg/kg) significantly reverses mechanical hypersensitivity in the more chronic models of Complete Freunds Adjuvant (CFA)–induced inflammatory pain and the spinal nerve ligation model of neuropathic pain. One hour post–oral administration, HC–030031 significantly reduces the lifting duration following 1% AITC injection (p<0.001)[2]. HC–030031completely reverses the enhanced mechanical firing in inflamed mice (p<0.001)[3].PROTOCOL (Extracted from published papers and Only for reference)Cell Assay: HC–030031 is prepared in DMSO and stored, and then diluted with appropriate medium (DMSO 0.4%) beforeuse [2].[2]HEK–293 cells stably expressing human TRPA1 are plated into 384–well plates at a density of 20,000 cells/well 24 hours prior to assaying. On the day of assay, cells are loaded with 4 μM Fluo–4 dye and 0.08% pluronic acid for 1 hour at room temperature in assay buffer consisting of Hank's balanced salt solution supplemented with 20 mM HEPES, 2.5 mM probenecid, and 4% TR–40.Calcium influx assays are performed using the Fluorometric Imaging Plate Reader (FLIPR) TETRA. Concentration–response curves are generated for the TRPA1 agonists cinnamaldehyde and AITC prior to antagonist testing so EC 60 concentrations could be determined.Titrations of HC–030031 are made from a DMSO stock solution and DMSO is kept to a constant of 0.4% in the assay. The antagonist is incubated with the cells for 10 minutes before the addition of an EC 60 concentration of either cinnamaldehyde (18 μM) or AITC (6μM) and calcium influx is monitored for an additional 10 minutes [2].Product Name:HC–030031Cat. No.:HY-15064CAS No.:349085-38-7Molecular Formula:C 18H 21N 5O 3Molecular Weight:355.39Target:TRP Channel Pathway:Membrane Transporter/Ion Channel Solubility:10 mM in DMSOAnimal Administration: HC–030031 is suspended in 0.5% methylcellulose (Rat)[2].HC–030031 is prepared in 0.5% DMSO and 0.25% Tween–80 in PBS (Mice)[3].[2][3]Rat[2]Male Sprague–Dawley rats (200–500 g) are used in all experiments. HC–030031 (100, 300 mg/kg) is used. For all experiments,HC–030031 is suspended in 0.5% Methylcellulose and the drug is dosed p.o. at a volume of 10 mL/kg. Naproxen (20 mg/kg) is dissolved in sterile water and dosed p.o. to serve as a positive comparator for the CFA experiment. Pregabalin (20 mg/kg) is dissolved in sterile water and dosed p.o. to serve as a positive comparator for the neuropathic pain experiment.Mice[3]Adult male C57BL/6 mice (8–12 weeks old) are used. Mice are injected with a 30 μL emulsion of undiluted CFA into the medial left plantar hind paw. The vehicle control group is injected with 30 μL of sterile 0.9% saline solution. Two days after injection, at the peak of hypersensitivity, the magnitude of inflammation is measured at the midpoint of the hind paw using digital calipers (VWR). For one experiment, the membrane–impermeable sodium channel inhibitor lidocaine N–ethyl–bromide, also known as QX–314, (0.2% in saline;30 μL) is injected with or without the TRPA1 agonist cinnamaldehyde (30 μM) into the left plantar hind paw 2 days post CFA injection. For another experiment, the TRPA1 antagonist HC–030031 (100 μg in 30 μL of 0.5% DMSO and 0.25% Tween–80 in PBS) is injected into the left plantar hind paw 2 days post CFA injection. Vehicle controls are injected with 30 μL 0.5% DMSO and 0.25% Tween–80 in PBS. All behavioral assays are completed between 1 and 4 hours following the QX–314, HC–030031 or vehicle injections. References:[1]. McNamara CR, et al. TRPA1 mediates formalin–induced pain. Proc Natl Acad Sci U S A. 2007 Aug 14;104(33):13525–30.[2]. Eid SR, et al. HC–030031, a TRPA1 selective antagonist, attenuates inflammatory– and neuropathy–induced mechanical hypersensitivity. Mol Pain. 2008 Oct 27;4:48.[3]. Lennertz RC, et al. TRPA1 mediates mechanical sensitization in nociceptors during inflammation. PLoS One. 2012;7(8):e43597.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

SCI340-HS SCI-S Magnetic Hotplate Stirrers User Ma

User ManualSCI340-HS Analog Hotplate Magnetic StirrerS CI-S Analog Magnetic StirrerPlease read the User Manual and the related Video of this instrument on our website carefully before use, and follow all operating and safety instructions!The website ContentsContents 1 Preface 2 Service 2 Warranty 21 Safety Instructions 32 Proper use 43 Inspection4 3.1 Receiving Inspection 43.2 Listing of Items 44 Trial run 55 Control and Display 56 Operation 67 Faults68 Maintenance and Cleaning 69 A ssociated Standards and Regulations 710 Technical data711 Products and Accessories 8PrefaceWelcome to the “MS-(H)-S Analog Hotplate Magnetic Stirrer User Manual”. Users should read this Manual carefully, follow the instructions and procedures, and beware of all the cautions when using this instrument.ServiceWhen help needed, you can always contact the sevice department of manufacturer for technical support in the following ways: SCILOGEX, LLC1275 Cromwell AveSuite C6Rocky Hill, CT 06067USATel：1- (860) 436-9221Fax：1- (860) 436-9745***************** Please provide the customer care representative with the following information:•Serial Number（on the rear panel）•Description of problem (i.e., hardware or software)•Methods and procedures adopted to resolve the problems •Your contact informationWarrantyYou have purchased a Scilogex instrument. This instrument is warranted to be free from defects in materials and workmanship under normal use and service, for a period of 24 months from the date of invoice. The warranty is extended only to the original purchaser. It shall not apply to any product or parts which have been damaged on account of improper installation, improper connections, misuse, accident or abnormal conditions of operation.For claims under the warranty please contact your local dealer. You may also send the instrument direct to our works, enclosing the invoice copy and by giving reasons for the claim. You would be solely liable for freight costs.1 Safety InstructionsWarning!•Read the operating instructions carefully before use the instrument.•Ensure that only trained staff work with the instrument.•Forbid to heat the substances with low- burning point or easy-volatile （MS-H-S）Risk of burn!•Caution when touching the housing parts and the heating plate. The heating plate can reach temperatures of 340 ℃.•Pay attention to the residual heat after switchingoff.Protective ground contact ！•Make sure that socket is earthed (protective ground contact) before use.•When work ，wear the personal guard to avoid the risk from:-Splashing and evaporation of liquids -Release of toxic or combustible gases.•Set up the instrument in a spacious area on an stable,clean, non-slip, dry and fireproof surface, do not operate the instrument in explosive atmospheres, with hazardoussubstances or under water.•Gradually increase the speed, reduce the speed if :-The stirring bar breakaway because of too high speed -The instrument is not running smoothly, or if the container moves on the base plate.•Temperature must always be set to at least 25 ℃ lower than the fire point of the media used.•Beware of hazards due to:-Flammable materials or media with a low boiling temperature -Overfilling of media -Unsafe container•Process pathogenic materials only in closed vessels.•If the case of the stirrer bar is PTFE,please note ：-Elemental fluorine, three fluoride and alkali metals will corrode the PTFE and Halogen alkanes make it expansion at room temperature- Molten alkali ，alkaline earth metals or their solution, as well as the powder in second and third ethnic of the periodic table of elements will have chemical reaction with PTFE when temperature reaches 300 ~ 400 ℃.• The voltage stated on the label must correspond to the main power supply.•Ensure that the mains power supply cable does not touch the hotplate. Do not cover the instrument.•The instrument may only be opened by experts.•Keep away from high magnetic field.•Observe the minimum distances between the devices, between the instruments and the wall and above theassembly (min. 100 mm).Figure 12 Proper useThe instrument is designed for schools, laboratories orfactories. This device is not suitable for use in residentialareas or other areas that may cause danger to the user orinstrument as mentioned in Chapter 1.3 Inspection3.1 Receiving InspectionUnpack the equipment carefully and check for any damageswhich may have arisen during transport. If it happens, pleasecontact manufacturer Limited for technical support.Note:If there is any apparent damage to the system,please do not plug it into the power line.3.2 Listing of ItemsThe packing includes the following items:Items QtyMain unit 1Power cable 15 Control and DisplayLED HeatHeating Knob (MS-H-S)Stirring KnobLED StirFigure 2Figure 3Stirrer bar 1User manual1Table 14 Trial run•Make sure the required operating voltage and power supply voltage match.•Ensure the socket must be earthed.•Ensure the power be off and the speed control knob and the temperature control knob to the lowest position •Plug in the power cable and power on the device.• Add the medium into the vessel with a stirring bar.•Put the vessel on the plate.•Set the rated stirring speed and the device begins to work.•Set the rated temperature and the device begins to work(MS-H-S).•Keep the temperature control knob and the speed control knob slowly to the lowest position to turn off the function.If these operations above are normal, the device is ready to operate. If these operations are not normal, the device may be damaged during transportation, please contact DragonLab corporation for technical support.Items DescriptionsStir knob Set the rated rotary speed in the safe stirring range from 0 to 1500 rpm. The function “Stirring” is switched ON or OFF via the knobHeat knob (MS-H-S)Set the rated temperature in the safe temperature range from room temperature to 340 ℃. The function “heating” is switched ON or OFF via the knob.LED heating Lit when heatingLED Power When the device is switched ON, theLED power is lit.Mains switch Switch ON or OFF.Table 26 Operation•Put the device on the stable and safe place，ensure the speed control knob and the temperature control knob to the lowest position and plug in the mains power.•Turn ON the power switch.•Turn the speed control knob regulation to set the rated rotary speed in the safe speed limit from 0 to 1500 rpm.•Turn the temperature control knob regulation to set the rated temperature in the safe limit from 0 to 340℃.(MS-H-S)•The instrument begins to work.Note：Forbid to transfer the vessel when the instrumentworking，or you must restart Stir function again,avoiding Stirring bar breakaway.7 Faults•If a unit fault happens, please power down the instrument.•Switch OFF the unit at the main ON/OFF switch for a few seconds.•The stirring function will continue to operate at the speed set before the fault took place.•The heating function will continue to operate at the set point before the fault took place.•If the problem is not solved, take the unit to your technical service center.8 Maintenance and Cleaning•Proper maintenance can keep instruments working in a good state and lengthen its lifetime.•Be careful not spray the cleanser into the instrument whencleaning.•Unplug the power line when cleaning.•Only use cleanser that we advised as below:Dyes Isopropyl alcoholConstruction materials Water containing tenside /isopropyl alcoholCosmetics Water containing tenside /isopropyl alcohol Foodstuffs Water containing tenside Fuels Water containing tensideTable 3•Before using other method for cleaning or decontamination, the user must ascertain with the manufacturer that this method does not harm or destroy the instrument.•Wear the proper protective gloves during cleaning of the instrument.•The instrument must be cleaned and put it into the initial packaging carton before sending to service for repair, avoiding the contamination of hazardous.•Use the instrument in a dry clean room and temperature stable environment.9 Associated standards and regulationsMachine guidelines: 73/023/EWG10 Technical dataItems ParametersV oltage [V AC] 200-240 / 100-120 Frequency [Hz]50/60Power [W]530(MS-H-S) / 130(MS-S) Stirring point positionquantity 1Max. stirring quantity (H2O) [L]20Max. magnetic bar [mm]80Motor type External rotorbrushless motorMax. power input of motor [W]18Max.power output of motor [W]10Speed range[rpm]0 ~ 1500Speed display accuracy [rpm]1Hotplate material Stainless steel/porcelain enamel Øof the hotplate [mm]Ø135Heating power[W]500(MS-H-S)Heating rate (1L water) [K/min]6(MS-H-S) Temperature range[℃]RT~340(MS-H-S)The safety temperature rangeof the hotplate [℃]350(MS-H-S) Dimensions (mm)280×160×85Weight [kg] 2.8Permitted ambienttemperature[℃] 5 ~ 40Permitted relative humidity80%Protection class acc. to DIN 60529IP42Table 4Accessories18900001MS 135.1 Carrier plate，Used withMS135.2--518900002MS 135.2 Quarter, 4 ml reaction vessel 18900003MS 135.3 Quarter, 20 ml reaction vessel 18900004MS 135.4 Quarter, 30 ml reaction vessel 18900005MS 135.5 Quarter, 40 ml reaction vessel 18900048MS 135.6 Quarter, 8 ml reaction vessel 18900049MS 135.7 Quarter, 16 ml reaction vessel 18900006Stirring bars（10mm x 6mm），1pcs/pk 18900007Stirring bars（15mm x 8mm），1pcs/pk 18900008Stirring bars（20mm x 8mm），1pcs/pk 18900009Stirring bars（25mm x 8mm），1pcs/pk 12500005Stirring bars（30mm x 6mm），1pcs/pk 12500005Stirring bars（30mm x 6mm），1pcs/pk 18900011Stirring bars（40mm x 8mm），1pcs/pk 12500004Stirring bars（50mm x 8mm），1pcs/pk 18900013Stirring bars（65mm x 8mm），1pcs/pk 18900014Stirring bars（80mm x 13mm），1pcs/pk 18900015Stirring bar mover，1pcTable 5SCILOGEX, LLC1275 Cromwell Ave.Suite C6Rocky Hill, CT 06067 USATel: +1(860) 436-9221Fax: +1(860) 436-9745*****************|。

PF-8380_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:PF–8380 is a potent autotaxin inhibitor with an IC 50 of 2.8 nM in isolated enzyme assay and 101 nM in human whole blood.IC50 & Target: IC50: 2.8 nM (Autotaxin)[1]In Vitro: PF–8380 also inhibits rat autotaxin with an IC 50 of 1.16 nM with FS–3 substrate. Potency of PF–8380 is maintained when using enzyme produced from fetal fibroblasts used in combination with LPC as a substrate. In human whole blood incubated with compound for 2 h autotaxin is inhibited with an IC 50 of 101 nM [1]. Autotaxin (ATX), an enzyme with lysophospholipase D (lysoPLD)activity, catalyzes the production of lysophosphatidic acid (LPA) from lysophosphatidylcholine (LPC). ATX is a 125 kDa autocrine tumor motility enzyme and is a member of the ectonuclease pyrophosphatase/phosphodiesterase (NPP) family. Pre–treatment of GL261 and U87–MG cells with 1 μM PF–8380 followed by 4 Gy irradiation results in decreased clonogenic survival, decreased migration (33% in GL261; P=0.002 and 17.9% in U87–MG; P=0.012), decreases invasion (35.6% in GL261; P=0.0037 and 31.8% in U87–MG; P=0.002), and attenuates radiation–induced Akt phosphorylation [2]. In Vivo: The pharmacokinetic profile of PF–8380 is evaluated at an intravenous dose of 1 mg/kg and oral doses of 1 to 100 mg/kg out to 24 h. PF–8380 has mean clearance of 31 mL/min/kg, volume of distribution at steady state of 3.2 L/kg, and effective t 1/2 of1.2 h. Oral bioavailability is moderate, ranging from 43 to 83%. Plasma concentrations increased with single oral escalating doses,but C max increased at a rate that is approximately proportional to dose from 1 to 10 mg/kg and less than proportional to dose from 10 to 100 mg/kg. PF–8380 exposures estimated by area under the curve are approximately proportional to dose and linear up to 100 mg/kg. Plasma C16:0, C18:0, and C20:0 LPA levels are measured immediately after collection. Maximal reduction of LPA levels is observed by the 3 mg/kg dose at 0.5 h with all LPA returning at or above baseline at 24 h [1]. Treatment with 10 mg/kg PF–8380increased tumor–associated vascularity modestly by 20% (P=0.497). When compared to control, treatment of PF–8380 45 min before 4 Gy irradiation decreased vascularity by nearly 48% when compared to control (P=0.031) and by 65% when compared to mice that received radiation alone (P=0.011)[2].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]FS–3 substrate is solubilized in assay buffer at 500 μM and frozen at –20°C in single–use aliquots for up to 4 weeks.Recombinant autotaxin is diluted in Tris–buffered saline (140 mM NaCl, 5 mM KCl, 1 mM CaCl 2, 1 mM MgCl 2, 50 mM Tris, pH 8.0)and incubated with compound in DMSO or DMSO alone (final 1% DMSO) for 15 min at 37°C, and the reaction is started with the addition of FS–3 at a final concentration of 1 μM. The reaction is allowed to proceed at 37°C for 30 min and monitored at 520 nm until the uninhibited control compared with a no–enzyme control gave a Z′≥0.5. IC 50s are determined in triplicate by using a four–parameter fit [1].Cell Assay: PF–8380 is dissolved in DMSO and stored, and then diluted with appropriate medium before use [2].[2]HUVEC (1×106) and bEnd.3 cells (1×106) are plated in 100 mm plates and after 24 h, U87–MG (2×106) and GL261 (2×106) cells are plated onto transwell inserts. After co–culture for 24 h, cells are treated with 1 μM of PF–8380 or vehicle control DMSO for 45 min prior to irradiation withProduct Name:PF–8380Cat. No.:HY-13344CAS No.:1144035-53-9Molecular Formula:C 22H 21Cl 2N 3O 5Molecular Weight:478.33Target:Phosphodiesterase (PDE)Pathway:Metabolic Enzyme/Protease Solubility:10 mM in DMSO0, 2, 4, 6, or 8 Gy. After the treatments as co–culture with either PF–8380 or DMSO calculated numbers of U87–MG and GL261 cells are plated to enable normalization for plating efficiencies. After 7 to 10 day incubation plates are fixed with 70% EtOH and stained with 1% methylene blue. Colonies consisting of>50 cells are counted by viewing the plates under a microscope. The survival fractions are calculated as (number of colonies/number of cells plated)/(number of colonies for corresponding control/number of cells plated). Survival curves are analyzed by curve fitting to the alpha/beta model calculating D0 and n[2].Animal Administration: PF–8380 is dissolved in DMSO and diluted with PBS or saline[1][2].[1][2] Rat[1]Male Lewis rats weighing 275 to 300 g are used and acclimated to their surroundings for approximately 1 week with food and water provided ad libitum. A minimum of 1 day before study, animals are anesthetized with isoflurane (to effect) and implanted with Culex vascular catheters in the carotid artery. Animals are acclimated in Culex cages overnight before dosing. Patency of the carotid artery catheter is maintained by using the “tend” function of the Culex automated blood sampler. Animals are dosed with PF–8380 at 1, 3, 10, 30, and 100 mg/kg by oral gavage after an overnight fast. Blood collections are obtained from the carotid artery and performed by the Culex automated blood sampler at 0.25, 0.5, 1, 2, 4, 6, 8, and 24 h after administration. Blood is centrifuged, and plasma is collected for analysis of PF–8380 and LPA concentrations.Mice[2]GL261 cells (1×106) are injected into the right hind limb of nude mice. Once tumors are palpable the mice are serpentine sorted into groups of six to seven animals representing similar distributions of tumor sizes (range=240 mm3). Tumor bearing mice are injected intraperitoneally with vehicle (DMSO) or PF–8380 at 10 mg/kg body weight once daily for five consecutive days. Forty five minutes after drug injection, mice are anesthetized with isoflurane and positioned in the RS2000 irradiator. They are then irradiated with 2 Gy daily for five consecutive days for a total of 10 Gy. Lead blocks (10 mm thick) are used to shield the head, thorax, and abdomen. Tumor size is monitored longitudinally using an external traceable digital caliper. Mice are sacrificed by cervical dislocation once the tumors reached a volume of approximately 10 mm3 or when ulceration became apparent on the hind limb per Animal Care guidelines. References:[1]. Gierse J, et al. A novel autotaxin inhibitor reduces lysophosphatidic acid levels in plasma and the site of inflammation. J Pharmacol Exp Ther. 2010 Jul; 334(1):310–7.[2]. Bhave SR, et al. Autotaxin Inhibition with PF–8380 Enhances the Radiosensitivity of Human and Murine Glioblastoma Cell Lines. Front Oncol. 2013 Sep 17; 3:236.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

SRPIN340_SDS_MedChemExpress

Inhibitors, Agonists, Screening LibrariesSafety Data Sheet Revision Date:May-24-2017Print Date:May-24-20171. PRODUCT AND COMPANY IDENTIFICATION1.1 Product identifierProduct name :SRPIN340Catalog No. :HY-13949CAS No. :218156-96-81.2 Relevant identified uses of the substance or mixture and uses advised againstIdentified uses :Laboratory chemicals, manufacture of substances.1.3 Details of the supplier of the safety data sheetCompany:MedChemExpress USATel:609-228-6898Fax:609-228-5909E-mail:sales@1.4 Emergency telephone numberEmergency Phone #:609-228-68982. HAZARDS IDENTIFICATION2.1 Classification of the substance or mixtureGHS Classification in accordance with 29 CFR 1910 (OSHA HCS)Acute toxicity, Oral (Category 4),H302Acute aquatic toxicity (Category 1),H400Chronic aquatic toxicity (Category 1),H4102.2 GHS Label elements, including precautionary statementsPictogramSignal word WarningHazard statement(s)H302 Harmful if swallowed.H410 Very toxic to aquatic life with long lasting effects.Precautionary statement(s)P264 Wash skin thoroughly after handling.P270 Do not eat, drink or smoke when using this product.P273 Avoid release to the environment.P301 + P312 IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.P330 Rinse mouth.P391 Collect spillage.P501 Dispose of contents/ container to an approved waste disposal plant.2.3 Other hazardsNone.3. COMPOSITION/INFORMATION ON INGREDIENTS3.1 SubstancesSynonyms:SRPIN 340; SRPIN⁻340; SRPK inhibitorFormula:C18H18F3N3OMolecular Weight:349.35CAS No. :218156-96-84. FIRST AID MEASURES4.1 Description of first aid measuresEye contactRemove any contact lenses, locate eye-wash station, and flush eyes immediately with large amounts of water. Separate eyelids with fingers to ensure adequate flushing. Promptly call a physician.Skin contactRinse skin thoroughly with large amounts of water. Remove contaminated clothing and shoes and call a physician.InhalationImmediately relocate self or casualty to fresh air. If breathing is difficult, give cardiopulmonary resuscitation (CPR). Avoid mouth-to-mouth resuscitation.IngestionWash out mouth with water; Do NOT induce vomiting; call a physician.4.2 Most important symptoms and effects, both acute and delayedThe most important known symptoms and effects are described in the labelling (see section 2.2).4.3 Indication of any immediate medical attention and special treatment neededTreat symptomatically.5. FIRE FIGHTING MEASURES5.1 Extinguishing mediaSuitable extinguishing mediaUse water spray, dry chemical, foam, and carbon dioxide fire extinguisher.5.2 Special hazards arising from the substance or mixtureDuring combustion, may emit irritant fumes.5.3 Advice for firefightersWear self-contained breathing apparatus and protective clothing.6. ACCIDENTAL RELEASE MEASURES6.1 Personal precautions, protective equipment and emergency proceduresUse full personal protective equipment. Avoid breathing vapors, mist, dust or gas. Ensure adequate ventilation. Evacuate personnel to safe areas.Refer to protective measures listed in sections 8.6.2 Environmental precautionsTry to prevent further leakage or spillage. Keep the product away from drains or water courses.6.3 Methods and materials for containment and cleaning upAbsorb solutions with finely-powdered liquid-binding material (diatomite, universal binders); Decontaminate surfaces and equipment by scrubbing with alcohol; Dispose of contaminated material according to Section 13.7. HANDLING AND STORAGE7.1 Precautions for safe handlingAvoid inhalation, contact with eyes and skin. Avoid dust and aerosol formation. Use only in areas with appropriate exhaust ventilation.7.2 Conditions for safe storage, including any incompatibilitiesKeep container tightly sealed in cool, well-ventilated area. Keep away from direct sunlight and sources of ignition.Recommended storage temperature:Powder-20°C 3 years4°C 2 yearsIn solvent-80°C 6 months-20°C 1 monthShipping at room temperature if less than 2 weeks.7.3 Specific end use(s)No data available.8. EXPOSURE CONTROLS/PERSONAL PROTECTION8.1 Control parametersComponents with workplace control parametersThis product contains no substances with occupational exposure limit values.8.2 Exposure controlsEngineering controlsEnsure adequate ventilation. Provide accessible safety shower and eye wash station.Personal protective equipmentEye protection Safety goggles with side-shields.Hand protection Protective gloves.Skin and body protection Impervious clothing.Respiratory protection Suitable respirator.Environmental exposure controls Keep the product away from drains, water courses or the soil. Cleanspillages in a safe way as soon as possible.9. PHYSICAL AND CHEMICAL PROPERTIES9.1 Information on basic physical and chemical propertiesAppearance White to light yellow (Solid)Odor No data availableOdor threshold No data availablepH No data availableMelting/freezing point No data availableBoiling point/range No data availableFlash point No data availableEvaporation rate No data availableFlammability (solid, gas)No data availableUpper/lower flammability or explosive limits No data availableVapor pressure No data availableVapor density No data availableRelative density No data availableWater Solubility No data availablePartition coefficient No data availableAuto-ignition temperature No data availableDecomposition temperature No data availableViscosity No data availableExplosive properties No data availableOxidizing properties No data available9.2 Other safety informationNo data available.10. STABILITY AND REACTIVITY10.1 ReactivityNo data available.10.2 Chemical stabilityStable under recommended storage conditions.10.3 Possibility of hazardous reactionsNo data available.10.4 Conditions to avoidNo data available.10.5 Incompatible materialsStrong acids/alkalis, strong oxidising/reducing agents.10.6 Hazardous decomposition productsUnder fire conditions, may decompose and emit toxic fumes.Other decomposition products - no data available.11.TOXICOLOGICAL INFORMATION11.1 Information on toxicological effectsAcute toxicityClassified based on available data. For more details, see section 2Skin corrosion/irritationClassified based on available data. For more details, see section 2Serious eye damage/irritationClassified based on available data. For more details, see section 2Respiratory or skin sensitizationClassified based on available data. For more details, see section 2Germ cell mutagenicityClassified based on available data. For more details, see section 2CarcinogenicityIARC: No component of this product present at a level equal to or greater than 0.1% is identified as probable, possible or confirmed human carcinogen by IARC.ACGIH: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by ACGIH.NTP: No component of this product present at a level equal to or greater than 0.1% is identified as a anticipated or confirmed carcinogen by NTP.OSHA: No component of this product present at a level equal to or greater than 0.1% is identified as a potential or confirmed carcinogen by OSHA.Reproductive toxicityClassified based on available data. For more details, see section 2Specific target organ toxicity - single exposureClassified based on available data. For more details, see section 2Specific target organ toxicity - repeated exposureClassified based on available data. For more details, see section 2Aspiration hazardClassified based on available data. For more details, see section 212. ECOLOGICAL INFORMATION12.1 ToxicityNo data available.12.2 Persistence and degradabilityNo data available.12.3 Bioaccumlative potentialNo data available.12.4 Mobility in soilNo data available.12.5 Results of PBT and vPvB assessmentPBT/vPvB assessment unavailable as chemical safety assessment not required or not conducted.12.6 Other adverse effectsNo data available.13. DISPOSAL CONSIDERATIONS13.1 Waste treatment methodsProductDispose substance in accordance with prevailing country, federal, state and local regulations.Contaminated packagingConduct recycling or disposal in accordance with prevailing country, federal, state and local regulations.14. TRANSPORT INFORMATIONDOT (US)This substance is considered to be non-hazardous for transport.IMDGUN number: 3077Class: 9Packing group: IIIEMS-No: F-A, S-FProper shipping name: ENVIRONMENTALLY HAZARDOUS SUBSTANCE, SOLID, N.O.S.Marine pollutant: Marine pollutant.IATAUN number: 3077Class: 9Packing group: IIIProper shipping name: Environmentally hazardous substance, solid, n.o.s.15. REGULATORY INFORMATIONSARA 302 Components:No chemicals in this material are subject to the reporting requirements of SARA Title III, Section 302.SARA 313 Components:This material does not contain any chemical components with known CAS numbers that exceed the threshold (De Minimis) reporting levels established by SARA Title III, Section 313.SARA 311/312 Hazards:No SARA Hazards.Massachusetts Right To Know Components:No components are subject to the Massachusetts Right to Know Act.Pennsylvania Right To Know Components:No components are subject to the Pennsylvania Right to Know Act.New Jersey Right To Know Components:No components are subject to the New Jersey Right to Know Act.California Prop. 65 Components:This product does not contain any chemicals known to State of California to cause cancer, birth defects, or anyother reproductive harm.16. OTHER INFORMATIONCopyright 2017 MedChemExpress. The above information is correct to the best of our present knowledge but does not purport to be all inclusive and should be used only as a guide. The product is for research use only and for experienced personnel. It must only be handled by suitably qualified experienced scientists in appropriately equipped and authorized facilities. The burden of safe use of this material rests entirely with the user. MedChemExpress disclaims all liability for any damage resulting from handling or from contact with this product.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

CH340 数据表说明书

USB to UART Bridge Controller CH340DataSheetVersion: 2F1. IntroductionCH340 is a USB bus converter chip, which converts USB to serial UART interface or to printer interface.In serial UART mode, CH340 provides common MODEM signal, to expand UART interface of computer or upgrade common serial devices to USB bus directly. For more information about converting USB to printer interface, please refer to the manual CH340DS2.2. Features● Full speed USB device interface, USB 2.0 compatible.● Emulate standard UART interface, used to upgrade the original serial peripherals or expand additionalserial UART via USB.● Original serial applications are totally compatible without any modification.● Hardware full duplex serial UART interface, integrated transmit-receive buffer, supports communicationbaud rate varies from 50bps to 2Mbps.● Supports common MODEM interface signals RTS, DTR, DCD, RI, DSR and CTS.● Provides further RS232, RS485, RS422 interface, etc. through external voltage conversion chip.● CH340R supports IrDA criterion SIR infrared communication, supports baud rate varies from 2400bps to115200bps.● Software compatible with CH341, use driver of CH341 directly.● Supports 5V and 3.3V power supply even 3V.● CH340C/N/K/E and CH340B have integrated 12MHz clock, no external crystal required, CH340B alsointegrates EEPROM used to configure the serial number, etc.● RoHS compliant SOP-16, SOP-8, SSOP-20 and ESSOP-10, MSOP-10 lead-free package.3. PackagesPackage Width Of Plastic Pitch Of Pin Instruction Of PackageOrdering InformationSOP-16 3.9mm 150mil 1.27mm 50mil Small outline 16-pin patch CH340G SOP-16 3.9mm 150mil 1.27mm 50mil Small outline 16-pin patch CH340C SOP-8 3.9mm 150mil 1.27mm 50mil Small outline 8-pin patch CH340NESSOP-10 3.9mm 150mil 1.00mm 39mil Shrink Small outline 10-pin patch withbackplaneCH340KSOP-16 3.9mm 150mil 1.27mm 50mil Small outline 16-pin patch CH340B MSOP-10 3.0mm 118mil 0.50mm 19.7mil Miniature Small outline 10-pin patch CH340E SSOP-20 5.30mm 209mil 0.65mm 25mil Shrink Small outline 20-pin patch CH340T SSOP-20 5.30mm 209mil 0.65mm 25mil Shrink Small outline 20-pin patch CH340RModel differences:CH340C, CH340N, CH340K, CH340E and CH340B have integrated clock, no external crystal required.CH340B has also integrated EEPROM used to configure the serial number, etc. Some functions can be customized.The CH340K has three diodes built in to reduce current flow backwards between the I/O pins of the MCU.The backplane of the CH340K is 0# pin GND, which is an optional connection; the 3# pin GND is the necessary connection.CH340R provides reverse polarity TXD and MODEM interface signals. (Discontinued)4. Pin OutSSOP20 Pin No. SOP16Pin No.ESSOP10Pin No.SOP8Pin No.PinNamePin TypePin Description (description in bracket is only aboutCH340R)19 16 7 5 VCC POWER Power supply voltage input, requires an external0.1uF decoupling capacitor8 1 3, 0 3 GND POWER Ground5 4 10 8 V3 POWER Connect to VCC when VCC is 3V3, connect to 0.1uF decoupling capacitor when VCC is 5V9 7 NONE NONEXI INCH340T/R/G: Input of crystal oscillator, connect to12MHz crystal and capacitorNC. NONE CH340C: No Connection, must be suspended RST# INCH340B: Input of external reset, active low,integrated pull-up resistor10 8 NONE NONEXO OUTCH340T/R/G: Output of crystal oscillator, connect tocrystal and capacitorOUT# OUTCH340C: MODEM output IO, software controlled,active lowNC. NONE CH340B: No Connection, must be suspended6 5 1 1 UD+ USB signal Connect to USB D+ Signal directly7 6 2 2 UD- USB signal Connect to USB D- Signal directly20 NONE NONE NONE NOS# IN Forbid USB device suspending, active low, integratedpull-up resistor3 2 8 6 TXD OUT Transmit asynchronous data output(reverse output forCH340R)4 3 9 7 RXD IN Receive asynchronous data input, integrated configurable pull-up and pull-down resistor11 9 5 NONE CTS# IN MODEM input signal, clear to send, active low(high)12 10 NONE NONE DSR# IN MODEM input signal, data set ready, activelow(high)13 11 NONE NONE RI# IN MODEM input signal, ring indicator , activelow(high)14 12 NONE NONE DCD# IN MODEM input signal, data carrier detect, activelow(high)15 13 4 NONE DTR# OUT MODEM output signal, data terminal ready, activelow(high)16 14 6 4 RTS# OUT MODEM output signal, request to send, activelow(high)2 NONE NONE NONE ACT# OUT USB configuration completed state output, active low18 15 NONE NONE R232 IN CH340T/R/G/C: Assistant RS232 enable, active high, integrated pull-down resistor17 15 NONE NONE TNOW OUTCH340T/E/B: Ongoing data transmission statusindicator, active highIR# INCH340R:Serial mode input setting, integrated pull-upresistor, SIR infrared serial interface when low,common serial interface when high1 NONE NONE NONE CK0 OUT CH340T: clock outputNC. NONE CH340R:No Connection, must be suspended5. Function DescriptionCH340 has integrated USB pull-up resistor, UD+ and UD- pins should be connected to USB bus directly.CH340 has integrated power-on reset circuit. CH340B also provides low active external reset pin.CH340G/CH340T/CH340R need to work with 12MHz clock signal supplied to XI pin. Generally, clock signal is generated by the inverter in CH340 through crystal oscillation. The peripheral circuit needs to place a crystal of 12MHz between XI and XO, and connect to a capacitor to ground separately.CH340C, CH340N, CH340K, CH340E and CH340B have integrated clock generator, no external crystal and oscillating capacitor required.CH340B also provides EEPROM for configuring data area, product serial number and other information could be customized for each chip by specific software tools, configurable data area is shown in the table below.Byte Address AbbreviationDescription Of Chip Configuration Data Area Default00H SIG For CH340B: internal configuration information valid reg,must be 58H.For CH340H/S: external configuration information validreg, must be 53H.Invalid for other value00H01H MODE Serial mode, must be 23H 23H02H CFGSpecific configuration of chip,bit5 is used to configure product Serial Number:0= valid; 1= invalid.FEH03H WP Internal configuration information write protect flag，57Himply read only, otherwise can be rewrite00H05~04H VID Vendor ID, high byte is behind, any value. Set to 0000H or0FFFFH implies VID and PID using vendor default value1A86H07~06H PID Product ID, high byte is behind, any value 7523H 0AH PWR Max Power, The maximum supply current in 2mA units 31H17~10H SN Serial Number, the length of ASCII string is 8, disable theSerial number when the first byte is not ASCII character(21H~7FH)123456783FH~1AH PRODFor CH340B: Product String, Unicode string for productdescription. The first byte is by total bytes (less than 26H),the next byte is 03H, Unicode string after that, using vendordefault description when do not meet characteristics above.Using productdefaultdescription whenthe first byte is00HOthers (Reserved) 00H or FFHCH340 supports 5V and 3.3V power voltage. When using 5V power supply, the VCC pin connects 5V power and the V3 pin should connect with decoupling 0.1uF capacitor. When using 3.3V power supply, connects V3 with VCC, both powered with 3.3V power supply, and the other circuit voltage which connected with CH340 cannot exceed 3.3V.CH340 supports USB device suspending automatically to save power. USB device suspend is forbidden when NOS# is driven low.The DTR# pin of CH340 is used as a configuration input pin before the USB configuration completion. An external 4.7KΩ pull-down resistor can be connected with this pin to generate default low during USB enumeration, to apply larger supply current to the USB bus via the configuration descriptor for CH340.In serial UART mode, CH340 contains these pins: data transfer pins, MODEM interface signals and assistant pins.Data transfer pins contain: TXD and RXD. RXD keeps high when UART reception is idle. For CH340G/C/T/R, If pin R232 is driven high, assistant RS232 function will be enabled, an internal inverter will automatically insert to the RXD, and the pin becomes low by default. When UART transmission is idle, the TXD of CH340G/C/N/E/B/T keeps high, CH340K is weak high, while CH340R keeps low.MODEM interface signals contain: CTS#, DSR#, RI#, DCD# and RTS#, CH340C also provides OUT# pin. All these MODEM interface signals are controlled and function defined by computer applications.Assistant pins contain: IR#, R232, CK0, ACT# and TNOW. When IR# is low, infrared serial interface mode is enabled. R232 is used to control assistant RS232 function. If R232 is driven high, the RXD input will be reversed automatically. ACT# is USB device configuration complete status output (such as USB infrared adapter ready). TNOW indicates CH340 is transmitting data from UART when it is high and becomes low when transmits over. In RS485 and other half-duplex mode, TNOW could be used to indicate UART transmit-receive status. IR# and R232 are detected only once when chip powered on and reset.CH340 has integrated separate transmit-receive buffer and supports simplex, half-duplex and full duplex UART communication. Serial data contains one low-level start bit , 5, 6, 7 or 8 data bits and 1 or 2 high-level stop bits, supports odd/even/mark/space check. CH340 supports common baud rate: 50, 75, 100, 110, 134.5, 150, 300, 600, 900, 1200, 1800, 2400, 3600, 4800, 9600, 14400, 19200, 28800, 33600, 38400, 56000, 57600, 76800, 115200, 128000, 153600, 230400, 460800, 921600, 1500000, 2000000 etc.The baud rate error of CH340 UART reception allows not less than 2%, the baud rate error of CH340G/CH340T/CH340R UART transmission is less than 0.3%, less than 1% for CH340C/CH340N/CH340K/CH340E/CH340B.In the Windows OS, CH340 driver can emulate standard serial port. So the mostly original serial applications are totally compatible, without any modification.CH340 can be used to upgrade the serial interface peripherals, or expand extra serial port for computers via USB bus, through external level conversion chip provide further RS232, RS485, RS422 interface, etc.Through extra infrared transceiver, CH340R can expand SIR infrared adapter for computer via USB bus, realize infrared communication between computers and peripheral equipment that comply with IrDA specifications.6. Parameters6.1. Absolute Maximum Ratings(critical state or exceeding maximum can cause chip to not work or even be damaged)Name Parameter Description Min. Max. UnitTA Operating AmbientTemperatureCH340G/CH340T/CH340R -40 85 ℃CH340C/CH340N/CH340K/CH340E/CH340B-20 70 ℃TS Storage Temperature -55 125 ℃VCC Supply Voltage(VCC connects to power, GND to ground) -0.5 6.0 V VIO The voltage of input or output pin -0.5 VCC+0.5 V6.2. Electrical Parameters (test conditions: TA=25,VCC=5V, exclude pin℃s connected to USB bus) (all the current parameters should multiply the coefficient of 40% when the supply voltage is 3.3V)Name Parameter Description Min. Typical Max. UnitVCC Supply VoltageV3 doesn’t connect to VCC 4.0 5 5.3V V3 connects toVCCCH340G/T/R 2.8 3.3 3.6CH340C/N/K/E/B 3.1 3.3 3.6ICCOperating SupplyCurrent(Normal Operation)CH340G/C/N/K/E/T/R7 20mACH340B 6 15ISLP Operating Supply Current(USBSuspend)VCC=5V 0.1 0.2mAVCC=3.3V 0.09 0.15VIL Input Low Voltage -0.5 0.7 V VIH Input High Voltage 2.0 VCC+0.5 V VOL Output Low Voltage(4mA draw current) 0.5 VVOH Output High Voltage(3mA output current)(Output 100uA current during chip reset)VCC-0.5 VIUP Draw current of input with integrated pull-up resistor 3 150 300 uAIDN Draw current of input with integrated pull-downresistor-50 -150 -300 uAVR Voltage threshold when power-up reset 2.4 2.6 2.8 V 6.3. Timing Parameters (test conditions: TA=25,VCC=5V)℃Name Parameter Description Min. Typical Max. Unit FCLK Frequency of input clock in XI 11.98 12.00 12.02 MHz TPR Reset time of power-up 20 35 50 mS 7. Applications7.1. USB to RS232 converter configurationThe image above use CH340T/CH340B (or CH340C ) to realize USB to RS232 converter. CH340 provides common UART and MODEM signals, converts TTL to RS232 through level conversion chip U8. Port P11 is DB9 connector, the pins and their functions are the same as common PC DB9 connector, the chips similar with U8 have MAX213/ADM213/SP213/MAX211 etc.U8 and C46/C47/C48/C49/C40 could be removed when realize USB to TTL converter only. The signal lines in the image only RXD、TXD and public ground need connected, the other signal lines should suspend when not use.P2 is USB port, USB bus contains a pair of 5V power lines and a pair of data signal lines . Usually, the color of +5V power line is red, the black one is ground. D+ signal line is green and the D- signal line is white. The max supply current of USB bus is up to 500mA. Generally, CH340 and low power consumption USB products can use the 5V power supplied by USB bus directly. If the USB products supply standing power by other manner, CH340 should use this power too. If the USB bus power and standing power are necessary at the same time, connect a 1Ωresistor between USB bus 5V power line and USB products 5V standing power line, and connect the ground lines of these two power directly.The capacitor C8 on V3 is 0.1uF, used to CH340 internal power node decoupling. The capacitor C9 is 0.1uF, used to external power decoupling.For CH340G/T/R, Crystal X2, capacitor C6 and C7 are used for clock oscillation circuit. The X2 is 12MHz quartz crystal, C6 and C7 are monolithic or high frequency ceramic capacitors with 22pF. If X2 is ceramic with low cost, C6 and C7 must use the recommended value of crystal manufacturer and generally is 47pF. For the crystalwhich is difficult to oscillate, halved value is suggested for C6.For CH340C/N/K/E/B, crystal X2 and capacitor C6, C7 are not required.When designing the PCB, pay attention to: decoupling capacitor C8 and C9 must keep near to connection pin of CH340; make sure D+ and D- signal lines are parallel and provide ground or pour copper on both sides to reduce outside interference; the signal lines relevant to XI and XO should be kept as short as possible. In order to reduce the high frequency interference, around the ground or pour copper around the relevant components.7.2. USB to RS232 converter configuration (3-wire)The image below is USB to 3-wire RS232 converter design which is the most basic and most commonly used, U5 uses MAX232/ICL232/SP232 etc.7.3. USB to RS232 converter configuration (simplified version using RS232)The image above is USB to RS232 converter design too, the function of this circuit is the same with 7.2 section except the range of output RS232 is narrower. When R232 pin is driven high, the assistant RS232 function will be enabled, just need to add some diodes, transistors, resistors and capacitors, the special level conversion chip U5 in section 7.2 could be replaced and the hardware cost is lower.7.4. USB to Infrared AdapterThe image above is a USB to infrared adapter design which is composed with USB convert IrDA infrared chip CH340R and infrared transceiver U14 (ZHX1810/HSDL3000 etc). The resistor R13 is used to weaken influence of large current in infrared transmitting. The current limiting resistor R14 should be adjusted according to the manufacturer’s recommended value of the infrared transceiver U14.7.5. USB to RS485 Converter ConfigurationThe TNOW pin can be used to control DE (high active send enable) and RE# (low active receive enable) pin of RS485 transceiver.3247.6. Connect CH340 to MCU and supply power togetherThe image below is a sample design to achieve USB connection on an MCU by connecting it to a CH340 via TTL serial port. Here we use self-power mode, VCC supports 5V or 3.3V(V3 shorted to VCC if VCC is 3.3V), and don’t use USB bus power VBUS at all(Can be tested by connecting series resistor to I/O of MCU if needed).CH340 shares the same power source with MCU, hence there would be no current inrush through I/O betweenCH340 and MCU.Unused CH340 pins can be suspended. For CH340C, CH340N, CH340E, CH340B, X6 , C17 and C18 are unused.7.7. Connect CH340 to MCU and supply power separatelyThe image below is a sample design to achieve USB connection on an MCU by connecting it to a CH340 via TTL serial port. CH340 is powered by USB bus VBUS. MCU is powered by another power source VDD, VDD supports 5V, 3.3V and even 2.5V, 1.8V. The diodes D6 and D7 are used to help relieve current inrush problems between CH340 and MCU through RXD or diode within RX. The RX pin of the MCU should enable internalpull-up resistor. If not, we suggest adding an 8kΩ ~ 30kΩ pull-up resistor to RX pin.Diode D6 is meant for circumstances when CH340 is not powered but MCU is powered, and TX high level causes current inrush through RXD internal diode; Diode D7 is meant for circumstances when MCU is not powered but CH340 is powered, and TXD high level causes current inrush through RX internal diode. If certain circumstance can be ensured to be avoided, the corresponding diode can be removed. For example, if the MCU has a permanent power source, then D7 can be short-circuited.Prioritized choice for diode is low power Schottky diode. Common diode such as IN4148 is also usable. Besides, replacing the diode with an 1kΩ(better less than 2kΩ) resistor is acceptable too.Usually, we don’t recommend power CH340 and MCU separately if not necessary.7.8. Connect CH340K to MCU and supply power separatelyThe figure above shows the reference circuit for USB communication between the MCU and the CH340K via UART. CH340K is powered by the USB bus VBUS (VCC), the MCU uses another power supply VDD, whichCH340 DataSheet(1st) 11supports 5V, 3.3V or even 2.5V, 1.8V. The backplane of the CH340K package is an optional GND pin that can be easily connected to GND or left floating depending on the PCB trace.The TXD and RTS# pins of the CH340K and the RXD pin have built-in diodes to prevent current flow backwards (as shown), and a weak pull-up resistor of about 75KΩis built in to maintain the default or idle state high level (in the figure). Not marked), this can achieve low-level drive and weak high-level drive, as well as reduce current flow backwards when the CH340K and MCU are independently powered. The CH340K can completely prevent the MCU power supply from powering down the current of the CH340K, and can also greatly reduce the current flow backwards of the MCU power supply to the power-off CH340K(up to 150μA). When used for communication baud rate above 120Kbps, it is recommended to enable built-in or external 2KΩ~ 22KΩpull-up resistor for the RX pin of the MCU.The DTR# pin of the CH340K is a normal push-pull output, and the CTS# pin is a normal input with a built-in pull-up resistor. These two pins have no built-in diodes and do not have the function of preventing current flow backwards. They are generally not used to connect to the MCU.DTR# can be used to control the power switch that VCC supplies to VDD. Four power control schemes are available as shown below. The T4 scheme and the Q1 scheme (Q1 should choose a lower Vth N-OSFET) is a simplified scheme. The VDD output voltage is about VCC-0.8V and the current does not exceed 200mA. The T6 scheme and the Q3 scheme are complete solutions. In the figure, D10 and D11 are used to prevent VDD from flow backwards to VCC, which is optional.。

胃癌组织中miRNA-340的表达变化及意义

医药２１０２年第５２卷第１４期
胃癌组织中ｍＲＡ３０的表达变化ｉＮ一４及意义
周兴舰，王君辅，谢勇。红浪李
（南昌大学第二附属医院，南昌３００）３０６
摘要：目的观察胃癌组织中微小ＲＡ（ｉＮＮｍＲＡ）－４３０的表达变化，探讨其临床意义。方法并
胃癌组织与癌旁正常胃黏膜组织采集后分别剪成１０ｃ０５ｃ × ．ｍ大小，ＲＡ保存液中，．ｍ× ．ｍ０５ｅ置Ｎ
一
由试剂盒自带。内参Ｕ６正向引物序列为５ＧＴＣ－ＴＧＣＧＡＡＡＡＴＡＡ．，向引物序列ＣＧＡＣＣＴＴＣＡＡＴ３反为５ＣＣＦＡＧＡＧＧＧＣＴ３。上述引物ＧＴＣＣＡ哪ＣＴＴＡ．
的溶液４Ｌ１、０×ＲｒｒＬ１Ｔｐｉｊ、０×Ｒｕｅｍｅ２ｘＴｂｆｒ２
Ｌ超纯ｄＴｉｔｒ１ｘ、Ｎｓｘ、ｕｎ、ＮＰＭｘｕｅＩＲａｉ１ｔＱａｔＬｎＬ
Ｒａｅ０５Ｌ加ＲａｅｆｅｄＨＯ至总容积为２Ｔｓ．，Ｎｓ—ｅｄ２ｒ０
的调控因子。成熟的ｍＲＡ以单链形式与辅酶因ｉＮ子ＴＢＲＰ和ＰＣＡＴ结合后形成ＲＡ诱导沉默复合Ｎ
ＲａｅｆｅＰＲ管中依次加入ＲＡ溶液８ＬＮｓｒＣｅＮ、
ＥｃｌＰｌ（．ｏｏｙＡ）Ｐｌｒｓ．Ｌ１ｏ（ｉｏｍｅａｅ０５ｙ、０ｘＰｌＡ）ｙＰｌｒｓｕｅ２］、 ×ｒＴｏｕｉｘ及ｏｙａｅＢｆｒｘ５ＡＰｓｌｔｎ４ｔｍｅＬｏＬ

SNS-032_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:SNS–032 is a selective inhibitor of cyclin–dependent kinase (CDK), inhibiting CDK2/7/9 with IC 50s of 48 nM/62 nM/4 nM.IC50 & Target: IC50: 48 nM (CDK2), 62 nM (CDK7), 4 nM (CDK9)In Vitro: SNS–032 has low sensitivity to CDK1 and CDK4 with IC 50 of 480 nM and 925 nM, respectively. SNS–032 effectively kills chronic lymphocytic leukemia cells in vitro regardless of prognostic indicators and treatment history. Compared with flavopiridol and roscovitine, SNS–032 is more potent, both in inhibition of RNA synthesis and at induction of apoptosis. SNS–032 activity is readily reversible; removal of SNS–032 reactivates RNA polymerase II, which led to resynthesis of Mcl–1 and cell survival [1].SNS–032 inhibits three dimensional capillary network formations of endothelial cells. SNS–032 completely prevents U87MGcell–mediated capillary formation of HUVECs. In addition, SNS–032 significantly prevents the production of VEGF in both cell lines,SNS–032 prevents in vitro angiogenesis, and this action is attributable to blocking of VEGF. Preclinical studies have shown that SNS–032 induces cell cycle arrest and apoptosis across multiple cell lines [2]. SNS–032 blocks the cell cycle via inhibition of CDKs 2and 7, and transcription via inhibition of CDKs 7 and 9. SNS–032 activity is unaffected by human serum [3]. SNS–032 induces a dose–dependent increase in annexin V staining and caspase–3 activation. At the molecular level, SNS–032 induces a marked dephosphorylation of serine 2 and 5 of RNA polymerase (RNA Pol) II and inhibits the expression of CDK2 and CDK9 anddephosphorylated CDK7[5].In Vivo: SNS–032 (15 mg/kg, i.p.) inhibits both xenografted BaF3–T674I cells and KBM5–T315I cells in vivo. SNS–032 abrogates the growth of tumors transplanted in nude mice with downregulation of T674I PDGFRα and T315I–Bcr–Abl [4].PROTOCOL (Extracted from published papers and Only for reference)Cell Assay:[2]Cell Titer–Glo (CTG) luminescent assay is performed to measure the growth curves of both HUVECs andU87MG cells. U87MG cells and HUVECs (2×103 cells/well) are seeded in a 96–well microplate in a final volume of 100 mL. After 24hours, cells are treated with various doses of SNS–032 (0–0.5 mM) for 24, 48, or 72 hours. After completion of the treatment, 100 mL of CTG solution is added to each well and incubated for 20 minutes at room temperature in the dark. Lysate (50 mL) is transferred to a 96–well white plate, and luminescence is measured by POLARstar OPTIMA. Percent cell growth is calculated by considering 100%growth at the time of SNS–032 addition.Animal Administration: SNS–032 is dissolved in tissue culture grade DMSO. [4]Nude nu/nu BALB/c mice are housed in barrier facilities with a 12–hour light–dark cycle, with food and water available ad libitum. A mixture of 1×107 of BaF3–T674I cells withMatrigel or KBM5–T315I cells (3×107) are inoculated subcutaneously on the flanks of 4– to 6–week–old male nude mice.Tumors are measured every other day with use of calipers. Tumor volumes are calculated by the following formula: a 2×b×0.4,where a is the smallest diameter and b is the diameter perpendicular to a. Four days after subcutaneous inoculation, when tumors are palpable (appr 100 mm 3), mice are randomized to receive treatment with vehicle (tissue culture medium containing DMSO 0.1%v/v) or SNS–032 (15 mg/kg injected intraperitoneally every 2 days) for about 2 weeks. SNS–032 is dissolved in tissue culture gradeProduct Name:SNS–032Cat. No.:HY-10008CAS No.:345627-80-7Molecular Formula:C 17H 24N 4O 2S 2Molecular Weight:380.53Target:CDK Pathway:Cell Cycle/DNA Damage Solubility:10 mM in DMSODMSO before dilution. The body weight, feeding behavior, and motor activity of each animal are monitored as indicators of general health. The animals are then euthanized, and tumor xenografts are immediately removed, weighed, stored, and fixed.References:[1]. Chen R, et al. Mechanism of action of SNS–032, a novel cyclin–dependent kinase inhibitor, in chronic lymphocytic leukemia. Blood. 2009 May 7;113(19):4637–45.[2]. Ali MA, et al. SNS–032 prevents tumor cell–induced angiogenesis by inhibiting vascular endothelial growth factor. Neoplasia. 2007 May;9(5):370–81.[3]. Conroy A, et al. SNS–032 is a potent and selective CDK 2, 7 and 9 inhibitor that drives target modulation in patient samples. Cancer Chemother Pharmacol. 2009 Sep;64(4):723–32.[4]. Wu Y, et al. Cyclin–dependent kinase 7/9 inhibitor SNS–032 abrogates FIP1–like–1 platelet–derived growth factor receptor α and bcr–abl oncogene addiction in malignant hematologic cells.Clin Cancer Res. 2012 Apr 1;18(7):1966–78. Epub 2012 Mar 23.[5]. Walsby E, et al. The cyclin–dependent kinase inhibitor SNS–032 has single agent activity in AML cells and is highly synergistic with cytarabine.Leukemia. 2011 Mar;25(3):411–9. Epub 2011 Jan 7.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

PCI-34051_DataSheet_MedChemExpress

Inhibitors, Agonists, Screening Libraries Data SheetBIOLOGICAL ACTIVITY:PCI–34051 is a potent and selective HDAC8 inhibitor with IC 50 of 10 nM, with >200–fold selectivity over the other HDAC isoforms.IC50 & Target: IC50: 10 nM (HDAC8), 2.9 μM (HDAC6), 4 μM (HDAC1), 13 μM (HDAC10)[1]In Vitro: PCI–34051 inhibits pure recombinant HDAC8 with K i of 10 nM with >200–fold selectivity over the other HDACs tested,including HDACs 1, 2, 3, 6 and 10. PCI–34051 is derived from a low molecular weight hydroxamic acid scaffold that possessed promising potency (HDAC8; K i =2 μM) and selectivity (approximately fivefold) for HDAC8 relative to the other class I HDACs.PCI–34051 is found to induce apoptosis at low micromolar concentrations in cell lines derived from T–cell lymphomas, including Jurkat and HuT78, whereas doses as high as 20 μM has no effect on B–cell– or myeloid–derived lymphomas or solid tumor lines [1]. In Vivo: Administration of PCI–34051 and Dexamethasone reduces the eosinophilic inflammation and airway hyperresponsiveness in asthma to reduce the airway remodeling [2].PROTOCOL (Extracted from published papers and Only for reference)Kinase Assay:[1]Histone deacetylase activity is measured using a continuous trypsin–coupled assay. For inhibitor characterization,measurements are performed in a reaction volume of 100 μL –1 using 96–well assay plates in a fluorescence plate reader. For each isozyme, the HDAC protein in reaction buffer (50 mM HEPES, 100 mM KCl, 0.001% Tween–20, 5% DMSO, pH 7.4,supplemented with bovine serum albumin at concentrations of 0–0.05% ) is mixed with inhibitor at various concentrations and allowed to incubate for 15 min. Trypsin is added to a final concentration of 50 nM, andacetyl–Gly–Ala–(N–acetyl–Lys)–amino–4–methylcoumarin is added to a final concentration of 25–100 μM to initiate the reaction.After a 30 min lag time, the fluorescence is measured over a 30 min time frame using an excitation wavelength of 355 nm and a detection wavelength of 460 nm. The increase in fluorescence with time is used as the measure of the reaction rate. Inhibition constants K i (app) are obtained using the program BatchK i [1].Cell Assay: PCI–34051 is prepared in DMSO and stored, and then diluted with appropriate medium before use [1].[1]Tumor cell lines and human umbilical vein endothelial cells are cultured for at least two doubling times, and growth is monitored at the end of compound exposure using an Alamar Blue fluorometric cell proliferation assay as recommended by the manufacturer. Compounds (e.g.,PCI–34051) are assayed in triplicate wells in 96–well plates. The concentration required to inhibit cell growth by 50% (GI 50) and 95% confidence intervals are estimated from nonlinear regression using a four–parameter logistic equation [1].Animal Administration: PCI–34051 is dissolved in DMSO and then diluted with PBS or saline [2].[2]Mice [2]A mouse model of asthma is utilized. Briefly, healthy female BALB/C mice (n=72) aged 6–8 weeks and weighing 18–22 g are used.Animals are housed independently in a pathogen–free room and provided ad libitum access to water and standard food. Animals are housed for 1 week prior to experiment onset. Mice are divided into six treatment groups: normal control, simple asthma,Dexamethasone, Tubastatin A HCl, PCI–34051, and Givinostat. Sensitization is carried out for mice in the last five groups on the 1st,8th and 15th day using ovalbumin (OVA, 20 μg) and aluminum hydroxide gel (2 mg). 7 days after the last sensitization, OVA (20Product Name:PCI–34051Cat. No.:HY-15224CAS No.:950762-95-5Molecular Formula:C 17H 16N 2O 3Molecular Weight:296.32Target:HDAC; HDAC Pathway:Epigenetics; Cell Cycle/DNA Damage Solubility:DMSO: ≥ 30 mg/mLmg/mL) atomization is performed using an ultrasonic atomizing device (3 mL/min for 30 min, 3 times/week for 8 weeks). Dexamethasone (2.0 mg/kg), TSA (0.5 mg/kg), PCI–34051 (0.5 mg/kg) and Givinostat (0.5 mg/kg) are administered via intraperitoneal injection 30 min before excitation. In the normal control group, normal saline is used instead of OVA.References:[1]. Balasubramanian S, et al. A novel histone deacetylase 8 (HDAC8)–specific inhibitor PCI–34051 induces apoptosis in T–cell lymphomas. Leukemia. 2008 May;22(5):1026–34.[2]. Ren Y, et al. Therapeutic effects of histone deacetylase inhibitors in a murine asthma model. Inflamm Res. 2016 Dec;65(12):995–1008.Caution: Product has not been fully validated for medical applications. For research use only.Tel: 609-228-6898 Fax: 609-228-5909 E-mail: tech@Address: 1 Deer Park Dr, Suite Q, Monmouth Junction, NJ 08852, USA。

CH340 USB到串行芯片数据手册说明书

USB to serial chip CH340English DataSheet Version: 2C1 IntroductionCH340 is a USB bus conversion chip, it can realize USB to UART interface or USB to printer interface.In serial UART mode, CH340 provides common MODEM liaison signal, used to expand UART interface of computer or upgrade the common serial device to USB bus directly. For more information on USB conversion to printer interface please refer to the manual CH340DS2.2 Features●Full speed USB device interface, compatible with USB V2.0.●Emulate standard UART interface, used to upgrade the serial peripherals, or expand UART interface viaUSB bus.●Original serial applications are totally compatible, without any modification in Windows operation system.●Hardware full duplex serial UART interface, built-in transmit-receive buffer, supports communication baudrate varies from 50bps to 2Mbps.●Supports common MODEM liaison signal RTS, DTR, DCD, RI, DSR and CTS.●Through external level conversion chip provide further RS232, RS485, RS422 interface, etc.●CH340R supports IrDA criterion SIR infrared communication, supports baud rate varies from 2400bps to 115200bps.●Software compatible with CH341, use driver of CH341 directly.●Support 5V and 3.3V power supply even 3V.●CH340C, CH340E and CH340B have built-in crystal, no external crystal, CH340B also integratesEEPROM used to configure the serial number, etc.●SOP-16 and SSOP-20 and MSOP-10 lead-free RoHS compliant package.3 PackagePackage shape Width of plastic Pitch of Pin Instruction of package Ordering type SOP-16 3.9mm150mil 1.27mm50mil Small outline package of 16-pin CH340G SOP-16 3.9mm150mil 1.27mm50mil Small outline package of 16-pin CH340C SOP-16 3.9mm150mil 1.27mm50mil Small outline package of 16-pin CH340B MSOP-10 3.0mm118mil0.50mm19.7mil Shrink small outline package of 10-pin CH340E SSOP-20 5.30mm209mil0.65mm25mil Shrink small outline package of 20-pin CH340T SSOP-20 5.30mm209mil0.65mm25mil Shrink small outline package of 20-pin CH340RModel differences:CH340C, CH340E and CH340B have built-in crystal, no external crystal;CH340B also has built-in EEPROM used to configure the serial number,etc.Some functions can be customized. CH340R provides reverse polarity TXD and MODEM signals. (No spot)4.PinsSSOP20 Pin No.SOP16Pin No.MSOP10Pin No.Pin Name Pin TypePin Description (description in bracket is only aboutCH340R)19167VCC POWER Positive power input port, requires an external 0.1uF powerdecoupling capacitor813GND POWER Public ground, ground connection for USB54NONE V3POWER Connect to VCC to input external power when 3.3V power supply, connect to 0.1uF decoupling capacitor when 5Vpower supplyXI IN CH340T/R/G: Input of crystal oscillator, connect to crystaland capacitorNC.NONE CH340C: No Connection, must be suspended 97NONERST#IN CH340B: Input of external reset, active low, built-inThe Data Sheet of CH340 (the first) 3pull-up resistorXO OUT CH340T/R/G: Output of crystal oscillator, connect tocrystal and capacitor108NONENC.NONE CH340C/B: No Connection, must be suspended 651UD+USB signal Directly connect to D+ data wire of USB bus 762UD-USB signal Directly connect to D- data wire of USB bus20NONE NONE NOS#IN Forbid USB device suspending, active low, built-in pull-upresistor328TXD OUT Transmit asynchronous data output(reverse output forCH340R)439RXD IN Receive asynchronous data input, built-in configurable pull-up and pull-down resistor1195CTS#IN MODEM liaison input signal, clear to send, activelow(high)1210NONE DSR#IN MODEM liaison input signal, data set ready, activelow(high)1311NONE RI#IN MODEM liaison input signal, ring indicator , activelow(high)1412NONE DCD#IN MODEM liaison input signal, data carrier detect, activelow(high)1513NONE DTR#OUT MODEM liaison output signal, data terminal ready, activelow(high)16144RTS#OUT MODEM liaison output signal, request to send, activelow(high)2NONE NONE ACT#OUT USB configuration completed state output, active low1815NONE R232IN CH340T/R/G/C: Assistant RS232 enable, active high,built-in pull-down resistorTNOW OUT CH340T/E/B: Ongoing data transmission status indicator,active high17156IR#IN CH340R:Serial mode input setting, built-in pull-up resistor, SIR infrared serial interface when low, common serialinterface when highCK0OUT CH340T: clock output 1NONE NONENC.NONE CH340R:No Connection, must be suspend5.Function DescriptionCH340 chip has built-in USB pull-up resistor, UD+ and UD- pins must be connected to USB bus directly.CH340 chip has built-in power-on reset circuit. CH340B also provides low active external reset pin.CH340G/CH340T/CH340R chips need to work with 12MHz clock signal supplied to XI pin. Generally, clock signal is generated crystal oscillation with inverter in CH340. The peripheral circuit needs to place a crystal of 12MHz between XI and XO, and connect to a capacitor to ground separately.CH340C, CH340E and CH340B chip have built-in clock generator, no external crystal and oscillating capacitor required.CH340B chip also provides EEPROM for configuring data area, product serial number and other information could be customized for each chip by specific software tools, configurable data area is shown in the table below.Byte Address AbbreviationDescription of chip configuration data area Default00H SIG For CH340B: Internal configuration information valid reg,must be 58H.For CH340H/S: External configuration information validreg, must be 53H.Invalid for other value00H01H MODE Serial mode, must be 23H23H02H CFGSpecific configuration of chip,bit5 is used to configure product Serial Number:0= valid; 1= invalid.FEH03H WP Internal configuration information write protect flag，57Himply read only, otherwise can be rewrite00H05~04H VID V endor ID, high byte is behind, any value. Set to 0000H or0FFFFH implies VID and PID using vendor default value1A86H07~06H PID Product ID, high byte is behind, any value7523H 0AH PWR Max Power, The maximum supply current in 2mA units31H17~10H SN Serial Number, the length of ASCII string is 8, disable theSerial number when the first byte is not ASCII character(21H~7FH)123456783FH~1AH PROD For CH340B: Product String, Unicode string for Productdescription. The first byte is by total bytes (less than 26H),the next byte is 03H, Unicode string after that, using vendordefault description when do not meet characteristics above.Using productdefault description when the first byte is00HOthers(Reserved unit)00H or FFHCH340 chip supports 5V and 3.3V power voltage. When using 5V source power, the VCC pin input 5V power and the V3 pin should connect with decoupling 0.1uF capacitor. When using 3.3V power voltage, connects V3 with VCC, both input 3.3V power voltage, and the other circuit voltage which connected with CH340 cannot exceed 3.3V.CH340 supports USB device suspending automatically to save power. USB device suspend is forbidden when NOS# is driven low.The DTR# pin of CH340 is used as a configuration input pin before the USB configuration is complete. An external 4.7KΩ pull-down resistor can be connected with this pin to generate default low level during USB enumeration, apply larger supply current to the USB bus via the configuration descriptor.In UART mode, CH340 chip contains these pins: data transfer pins, MODEM liaison signal pins and assistant pins.Data transfer pins contain: TXD and RXD pin. RXD keeps high when UART reception is idle. For CH340G/C/T/R chip, If R232 pin is driven high, assistant RS232 function will be enabled, an internal inverter will automatically insert to the RXD pin , and the pin becomes low by default. When UART transmission is idle, the TXD pin of CH340G/C/B/T keeps high, while CH340R keeps low. MODEM liaison signal pins contain: CTS#, DSR#, RI#, DCD# and RTS#. All these MODEM liaison signal are controlled and function defined by computer applications.Assistant pins contain: IR#, R232, CK0, ACT# and TNOW. When IR# is low-level, infrared serial interface mode is enabled. R232 pin is used to control assistant RS232 function. If R232 pin is driven high, the RXD pin input will be reversed automatically. ACT# pin is USB device configuration complete status output (such as USB infrared adapter ready). TNOW pin indicates CH340 is transmitting data from UART when it is high-level and becomes low when transmit over. In RS485 and other half-duplex mode, TNOW could be used to indicate UART transmit-receive status. IR# and R232 are detected only once when chip powered on and reset.CH340 has built-in separate transmit-receive buffer and supports simplex, half-duplex and full duplex UART communication. Serial data contains one low-level start bit , 5, 6, 7 or 8 data bits and 1 or 2 high-level stop bits, supports odd/even/mark/space check. CH340 supports common baud rate: 50, 75, 100, 110, 134.5, 150, 300, 600, 900, 1200, 1800, 2400, 3600, 4800, 9600, 14400, 19200, 33600, 38400, 56000, 57600, 76800, 115200, 128000, 153600, 230400, 460800, 921600, 1500000, 2000000 etc.The baud rate error of CH340 UART reception allows not less than 2%, the baud rate error of CH340G/CH340T/CH340R UART transmission is less than 0.3%, less than 1% for CH340C/CH340E/ CH340B.In the Windows operation system, CH340 driver can emulate standard serial port. So the mostly original serial applications are totally compatible, without any modification.CH340 can be used to upgrade the serial interface peripherals, or expand extra serial port for computers via USB bus, through external level conversion chip provide further RS232, RS485, RS422 interface, etc.Only need to add infrared transceiver, CH340R can expand SIR infrared adapter for computer via USB bus, realize infrared communication between computer and peripheral equipment that comply with IrDA specifications.6.Parameter6.1 Absolute maximum rating (Critical or exceeding absolute maximum can cause permanent damage to device)Name Parameter Description Min.Max.UnitsCH340G/CH340T/CH340R-4085℃TA Ambient temperatureCH340C/CH340E/CH340B-2070℃TS Storage temperature-55125℃VCC Supply V oltage(VCC connects to power, GND to ground)-0.5 6.0VVIO The voltage of input or output pin-0.5VCC+0.5V6.2. Electrical Parameter (test conditions: TA=25℃, VCC=5V, exclude pin connected to USB bus)(All the current parameters should multiply the coefficient of 40% when the power is 3.3V)NameParameter DescriptionMin. Typical Max. Units V3 doesn’t connect to VCC 4.0 5 5.3 CH340G/T/R 2.8 3.3 3.6 VCC Supply V oltageV3 connect toVCCCH340C/E/B 3.03.3 3.6 VCH340G/C/E/T/R7 20 ICCOperating Supply Current(Normal Operation)CH340B 6 15 mA VCC=5V 0.1 0.2 ISLPOperating Supply Current(USBSuspend) VCC=3.3V0.090.15 mA VIL Low-level Input V oltage -0.5 0.7 V VIH High-level Input V oltage2.0 VCC+0.5 V VOL Low-level Output V oltage(4mA draw current) 0.5V VOH High-level Output V oltage(3mA output current) (Output 100uA current during chip reset) VCC-0.5 V IUP Input current input with built-in pull-up resistor 3 150 300 uA IDN Input current input with built-in pull-down resistor-50 -150 -300 uA VRRestrict voltage when power-up reset2.42.62.8V6.3. Sequence Parameter (test conditions: TA=25℃,VCC=5V)Name Parameter Description Min. Typical Max. Units FCLK Frequency of input clock in XI 11.98 12.00 12.02 MHz TPRReset time of power-up203550mS7.Application7.1.1 USB to RS232 Converter Configuration using CH340T7.1.2 USB to RS232 Converter Configuration using CH340BThe image above use CH340T/CH340B (or CH340C ) to realize USB to RS232 converter. CH340 provides common UART and MODEM signal, converts TTL to RS232 through level conversion chip U8. Port P11 is DB9 connector, the pin and its function are the same as common PC DB9 connector, the chips similar withU8 have MAX213/ADM213/SP213/MAX211 etc.U8 and C46/C47/C48/C49/C40 could be removed when realize USB to TTL converter only. The signal lines in the image only RXD、TXD and public ground need connected, the other signal lines should suspend when not use.P2 is USB port, USB bus contains a pair of 5V power lines and a pair of data signal lines . Usually, the color of +5V power line is red, the black one is ground. D+ signal line is green and the D- signal line is white. The max supply current of USB bus is up to 500mA. Generally, CH340 and low power consumption USB products can use the 5V power supplied by USB bus directly. If the USB products supply standing power by other manner, CH340 should use this power too. If the USB bus power and standing power are necessary at the same time, connect a 1Ω resistor between USB bus 5V power line and USB products 5V standing power line, and connect the ground lines of these two power directly.The capacitor C8 on V3 pin is 0.1uF, used to CH340 internal power node decoupling. The capacitor C9 is 0.1uF, used to external power decoupling.For CH340G/T/R chip, Crystal X2, capacitor C6 and C7 are used for clock oscillation circuit. The X2 is 12MHz quartz crystal, C6 and C7 are monolithic or high frequency ceramic capacitors with 22pF. If X2 is ceramic with low cost, C6 and C7 must use the recommended value of crystal manufacturer and generally is 47pF. For the crystal which is difficult to oscillate, halved value is suggested for C6.For CH340C/E/B chip, crystal X2 and capacitor C6, C7 are not required.When designing the PCB, pay attention to: decoupling capacitor C8 and C9 must keep near to connection pin of CH340; makes sure D+ and D- signal lines are parallel and supply ground lines or pour copper beside them to decrease the interference from outside signal; the signal lines relevant to XI and XO should be kept as short as possible. In order to lessen the high frequency interference, around the ground wire or pour copper around the relevant components.7.2. USB to RS232 Converter Configuration (3-wire)The image below is USB to 3-wire RS232 converter design which is the most basic and most commonly used, U5 uses MAX232/ICL232/SP232 etc.7.3 USB to RS232 Converter Configuration (Simplified version using R232)The image above is USB to RS232 converter design too, the function of this circuit is the same with 7.2 section except the range of output RS232 is narrower. When R232 pin is driven high, the assistant RS232 function will be enabled, just need to add some diodes, transistors, resistors and capacitors, the special level conversion chip U5 in section 7.2 could be replaced and the hardware cost is lower.7.4 USB to Infrared AdapterThe image above is USB to infrared adapter design is composed with USB convert IrDA infrared chip CH340R and infrared transceiver U14 (ZHX1810/HSDL3000 etc).The resistor R13 is used to weaken influence of large current in infrared transmitting. The current limiting resistor R14 should be adjusted according to the manufacturer’s recommended value of the infrared transceiver U14.7.5. USB to RS485 Converter ConfigurationThe TNOW pin can be used to control DE (high active send enable) and RE# (low active receive enable)pin of RS485 transceiver.。