MALDI_TOF详解

1.MALDI-TOF MS在该工作中的作用 2.用了什么原理或者思路怎么来的 3.你有什么收获
MALDI-TOF MS在该工作中的作用
• 通过MALDI-TOF MS和数据库查询的多肽位置绘制在现代 蛋白质组分分析中是一种主要且广为人知的分析工具。 • 尽管MALDI-TOF MS对痕量的多肽和蛋白质高度敏感，但 由于那些提取于生物组织中的低丰度的多肽或者蛋白质不 仅含量非常低（<1nM）,而且它们的质谱信号会受到高丰 度蛋白质和在预处理过程中引入样品的杂质的强烈干扰， 以至于MALDI-TOF MS对其分析的效率似乎很低。
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Because the Fe3O4@SiO2@PMMA microspheres possess a strong magnetic core and an organic hydrophobic PMMA exterior, they hold great promise for rapid magnetic separation and high absorption of trace peptides or proteins in solution-phasesystems. In fact, we found that Fe3O4@SiO2@PMMA micro-spheres exhibit some remarkable features that are useful for the extraction of peptides and proteins and thus their analysis by MALDI-ToF MS. 由于Fe3O4@SiO2@PMMA微球具有强力的磁性核心以及疏水的有机表面， 所以它具有很大的潜力用于在溶液相的体系中进行快速地磁性分离和对多肽 和蛋白质的高效吸收，事实上我们发现Fe3O4@SiO2@PMMA微球具有突出 的性质因此能有效地用于多肽和蛋白质的提取，和用于MALDI-ToF MS分析。
magnetic nanomaterials的合成思路
• • • • • 这篇文献提到最近一些研究尝试利用磁性纳米材料解决MALDI-TOF MS对低丰度多肽/蛋白质分析效率低的这一问题， 磁性纳米材料具有很强的电磁特性使得纳米材料靶向的多肽/蛋白质的复合物容易从样品溶液中分离。 PMMA can be used as a powerful absorbent for the enrichment of peptides and proteins ，the design and synthesis of magnetic nanomaterials with PMMA at the surface 从前人的研究知道PMMA可用作多肽和蛋白质的富集的强力吸收剂 以磁性粒子为核构筑Fe3O4 @SiO2@PMMA 核壳壳复合微球，在MALDI-TOF质谱分析中对多肽和蛋白质富集的应 用。 PMMA can be used as a powerful absorbent for the enrichment of peptides and proteins ，the design and synthesis of magnetic nanomaterials with PMMA at the surface 从前人的研究知道PMMA可用作多肽和蛋白质的富集的强力吸收剂 Herein we report the facile synthesis of core–shell–shell composite Fe3O4 @SiO2@PMMA microspheres by combining a sol-gel approach with a seeded aqueous-phase radical polymeri-zation method, along with application of the microspheres to the enrichment of peptides and proteins for mass spectro-metric analysis. 这篇文章指出作者探索了一种灵巧合成方法来合成Fe3O4 @SiO2@PMMA 核壳壳结构的复合微球，这种方法包含 了一个溶胶-凝胶步骤和一个种子水相自由基聚合步骤，以及在质谱分析中对多肽和蛋白质富集的应用 magnetic nanomaterials 磁性纳米材料
原理
what i learn from the paper
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transmission electron microscopy(TEM) shows that the magnetite particles obtained are nearly spherical in shape and have an average diameter of ca.170 nm 透射电镜的表征显示获得的磁性微粒接近球形，并且具有170nm的平均直径。 TEM images of higher magnification reveal that each magnetite particle is composed of many smaller magnetite nanoparticlesof about 10 nm diameter (Figure S1, Supporting Informa-tion). A representative TEM image of the obtainedFe3O4@Si O2core–shell microspheres (Figure 1 b) shows that the dark magnetite particles are individually coated with a uniform gray silica shell with a thickness of ca. 35 nm.Through a robust sol-gel approach, the thickness of the silicashell can be tuned from tens to hundreds of nanometers. 更高分辨率的透射电镜的图形显示每一个磁性颗粒由更小的直径为10nm的纳米颗粒组 成 TEM images (Figure 1 c [inset] and 1 d) reveal a sandwich structure of thecomposite microspheres with a dark Fe3O4core, a gray SiO2 middle layer, and a light-gray PMMA shell about 20 nm in thickness
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Magnetic silicananospheres with a highly ordered periodic mesostructure have been synthesized for size-selective bioseparation of proteins. • 合成了具有高度有序的周期性介孔的磁性sio2纳米微球，用在了蛋白质的生 物分离中的尺寸选择。 ① according to a previously reported method，magnetite core–shell Fe3O4Si O2microspheres were synthesized by a sol-gel process to coat a thin layer of dense amorphous silica oபைடு நூலகம் Fe3O4 particles. 根据已报道的合成方法，磁性的核壳结构Fe3O4SiO2微球通过溶胶凝胶步骤合 成后在Fe3O4粒子上涂覆了一层薄薄的粘稠的sio2层 ① The Fe3O4@SiO2microspheres were then modified with 3methacryloxypropyltrimethoxysilane(MPS即KH-570，一种可聚合的硅烷偶 联剂) 然后用MPS（一种可聚合的硅烷偶联剂)）修饰Fe3O4@SiO2微球 ① An aqueous-phase radical polymerization of methyl methacrylate (MMA) was then carried out in the presence of Fe3O4@SiO2–MPS microspheres, resulting in Fe3O4@SiO2@PMMA microspheres with a well-defined core– shell structure. ② 在含有Fe3O4@SiO2–MPS微球的溶液中进行MMA的水相自由基聚合，使得 Fe3O4@SiO2@PMMA 微球具有明确的核壳结构。
由于fe3o4sio2pmma微球具有强力的磁性核心以及疏水的有机表面所以它具有很大的潜力用于在溶液相的体系中进行快速地磁性分离和对多肽和蛋白质的高效吸收事实上我们发现fe3o4sio2pmma微球具有突出的性质因此能有效地用于多肽和蛋白质的提取和用于malditofms分析
Synthesis of Fe3O4@SiO2@PMMA Core–Shell–Shell Magnetic Microspheres for Highly Efficient Enrichment of Peptides and Proteins for MALDI-To F MS Analysis