氯沙坦对慢性不可预知温和应激模型大鼠的抗抑郁作用

TNF-a表达量、SOD活性、MDA含量差异均无统计学意义(Q0.05)。CUMS Control组糖水的消
耗量、
的、
)SOD活性
，
不
)Ang
I含量和AT1R、IL-6、TNF-a表达量及MDA含量显著升高，差异具有统计学意义(！＜0.05)。CUMS+
Los
CUMS 糖水的
、
的、
、SOD 性
,
而强迫游泳不动时间、Ang I含量和AT1R、IL-6、TNF-"表达量及MDA含量均降低，差
【摘要】目的探讨氯沙坦(Los)对慢性不可预知温和应激(CUMS )模型大鼠抑郁行为的影
响，对其抗炎和抗氧化作用进行考察，探究其潜在的抗抑郁 。方法 24只SD大鼠
为4 :对照组(Control))氯沙坦组(Los)、抑郁组(CUMS)、抑郁+沙坦组(CUMS+Los),每组6 。
Los组)CUMS+Los组每天灌胃10 mg/kg的Los溶液,Control组和CUMS组每日灌胃等体积的生理
计学 (！＜0.05)。结论Los能明显改善CUMS所致的大鼠抑郁样行为， 炎症反应、减轻氧
化损伤 ，且该作用与脑内肾-
系 关，为抗抑郁的靶向治疗提供了新的方向。
【关键词】氯沙坦；慢性不可预知温和应激；抑郁
Anti-depressant effects of losartan on chronic unpredicted mild stress induced depression rat model Tian Guiqin1, Zhang Jin—* Zhu Xin-2, Chen You2* Wang Changshui3. ^Department of Pharmacy, Jining N0.4 People *s Hospital* Jining 272031 China; 'Department of Medical Engineering* Jining Medical University* Jining 272067* China; 3Department of Clinical Translational Medicine* Jining Life Science Center* Jining 272104* China Corresponding author: Wang Changshui* Email wangchangshui88@
D0I:10.3877/cma.j.issn.2095-123X.2019.05.010 作者单位：272031济宁市第四人民医院药剂科1；272067济宁，济宁医学院医药工程学院2；272104济宁 市生命科学中心临床转化药学研究院3 通信作者：王长水，Email: wangchangshui88@
中华脑科疾病与康复杂志(电子版)2019 年 10 月第 9 卷第 5 期 Chin J —ra.in Dii RehaLil (Electronic Edition), Oct. 2019, VoL9, No.5 - 297 •
•基础研究•
氯沙坦对慢性不可预知温和应激模型大鼠 的抗抑郁作用
田桂芹1张静2祝幸2陈友2王长水B
盐水，连续8周。用糖水
)
和
鼠抑郁 行为。联
鼠
II(Ang :D)的含量。荧光定量PCR
! 1型
受体(AT1R)和炎性因子白细胞介素6(IL-6))
子a(TNF-a)的表达量。分光光度法检测
氧化 化酶(SOD)性来自(MDA)含量。结果Los
Control糖水的
)进
的 、进
、
不动时间、Ang I含量、AT1R、IL-6、
[Abstract] Objective To investigate effect of losartan (Los) on the behavior change of chronic unpredicted mild stress (CUMS) model rats, anti-inflammatory and antioxidant action, and explore its potential antidepressant mechanism. Methods Sprague-Dawley rats were randomly divided into 4 groups: Control group, Los group, CUMS group, and CUMS +Los group. The rats in Los group and CUMS +Los group were continuously administrated with losartan (10 mg/kg) by gavage and those in Control group and CUMS group received an equal volume of saline for 8 weeks. Sucrose preference test (SPT), forced swim test (FST) and elevated plus maze (EPM) were performed to evaluate depression-like behaviors in rats. Enzyme-linked immunosorbent assay was used to examine angiotensin I (Ang I ) levels. RT-PCR was used to detect the mRNA expression level of Ang I type 1 receptor (AT1R), interleukin-6 (IL-6) and tumor necrosis factor " (TNF-a). The activity of superoxide dismutase (SOD) and content of malonyldialdehyde (MDA) by spectrophotometry. Results There was no significant