食用菌无菌操作流程

食用菌无菌操作流程
食用菌无菌操作是指在无菌条件下进行的一系列操作步骤。

Sterile operation of edible fungi refers to a series of operations conducted under sterile conditions.
首先要准备好无菌操作所需的器皿和工具。

First, prepare the utensils and tools needed for sterile operation.
在操作前，要洗手并穿戴好无菌衣物和手套。

Before operation, wash hands and wear sterile clothing and gloves.
将操作台面用75%的酒精擦拭消毒。

Wipe the operating table with 75% alcohol for disinfection.
将工具和器皿放入高压蒸汽灭菌锅中高压蒸汽灭菌。

Place the tools and utensils in a high-pressure steam sterilizer for high-pressure steam sterilization.
待工具和器皿冷却后，将其取出放在无菌操作台上。

After the tools and utensils are cooled, remove them and place them on the sterile operating table.
将需要使用的菌种培养基半固体化后装入培养皿中。

After semi-solidifying the culture medium, fill it into the petri dishes for use.
将菌种转移到无菌操作台上。

Transfer the strains to the sterile operating table.
打开紫外线灯，让其照射操作区域15-30分钟。

Turn on the ultraviolet lamp and let it shine on the operating area for 15-30 minutes.
做好无菌操作前的准备后，穿戴好口罩眼镜，开始无菌操作。

After preparing for sterile operation, wear masks and glasses and start sterile operation.
打开无菌工作台上的风淋，待其排出的空气符合无菌要求后再进行操作。

Turn on the air shower on the sterile workbench, and start operation after the discharged air meets sterile requirements.
在无菌操作中，要保持操作环境的清洁和整洁。

During sterile operation, keep the operating environment clean and tidy.
操作时要注意动作轻柔，避免造成空气的扰动。

During operation, be gentle to avoid disturbing the air.
将培养皿拿起后盖上盖子，避免外界空气和细菌污染。

After picking up the petri dish, cover it to avoid contamination by external air and bacteria.
每次操作完毕，要及时清理工作台和器皿。

After each operation, clean the workbench and utensils in time.
将使用过的器皿和工具进行高压蒸汽灭菌处理。

Sterilize the used utensils and tools with high-pressure steam.
无菌操作中要严格遵循操作规程，确保无菌操作的可靠性。

Strictly follow the operating procedures during sterile operation to ensure the reliability of sterile operation.
在操作结束后，要及时清洁消毒无菌操作台和周围区域。

After the operation, clean and disinfect the sterile operating table and the surrounding area in time.
所有的无菌操作都应该在专门的无菌室内进行。

All sterile operations should be carried out in a dedicated sterile room.
无菌操作是食用菌培养的重要环节，对培养出高质量的菌种有着关键性作用。

Sterile operation is an important part of edible fungi cultivation and plays a crucial role in cultivating high-quality strains.
严格控制无菌条件，是保证食用菌培养过程中不受细菌污染的关键。

Strictly controlling sterile conditions is the key to ensuring that edible fungi cultivation is not contaminated by bacteria.
无菌操作的每个细节都需要严格执行，不能有丝毫马虎。

Every detail of sterile operation needs to be strictly implemented without any carelessness.
无菌操作需要操作者具备一定的专业知识和技能。

Sterile operation requires operators to have certain professional knowledge and skills.
在无菌操作过程中，要密切关注无菌条件的变化。

During sterile operation, pay close attention to changes in sterile conditions.
只有确保无菌操作的严谨性，才能保证培养出无菌纯种的食用菌菌种。

Only by ensuring the rigor of sterile operation can we cultivate sterile strains of edible fungi.
无菌操作过程中，要避免操作者的喷嚏或吹气直接对菌种进行呼吸。

During sterile operation, avoid sneezing or blowing directly on the strains.
无菌操作的手法要求熟练且规范。

The technique of sterile operation requires proficiency and standardization.
无菌操作要求操作者保持警觉，随时注意操作环境的变化。

Sterile operation requires operators to remain vigilant and pay attention to changes in the operating environment at all times.
要保证无菌操作室内通风良好，避免操作区域内空气的滞留。

Ensure good ventilation in the sterile operation room to avoid air stagnation in the operating area.
为了提高食用菌培养的成功率，无菌操作至关重要。

To improve the success rate of edible fungi cultivation, sterile operation is crucial.
无菌操作的关键是要保持操作区域内的环境完全无菌。

The key to sterile operation is to maintain a completely sterile environment in the operating area.
无菌操作涉及到菌种的分离、传代、保存等多项操作。

Sterile operation involves multiple operations such as isolation, passage, and preservation of strains.
无菌操作要求操作者具备良好的耐心和细心。

Sterile operation requires operators to have good
patience and meticulousness.
在进行无菌操作时，要注意隔离不同种类的菌株，避免交叉污染。

When conducting sterile operation, pay attention to isolating different strains to avoid cross-contamination.
无菌操作可以有效避免细菌对食用菌培养的干扰。

Sterile operation can effectively avoid bacterial interference with edible fungi cultivation.
无菌操作需要操作者具备一定的操作经验和技巧。

Sterile operation requires the operator to have a certain level of operational experience and skills.
在无菌操作中，要及时清理物品，避免操作区域内产生细菌污染。

In sterile operation, clean up items in a timely manner
to avoid bacterial contamination in the operating area.
无菌操作是食用菌培养工作中的一个重要环节。

Sterile operation is an important part of edible fungi cultivation.
为了保证食用菌的质量和产量，无菌操作必不可少。

To ensure the quality and yield of edible fungi, sterile operation is essential.
无菌操作对于食用菌的培养有着不可替代的作用。

Sterile operation plays an irreplaceable role in the cultivation of edible fungi.
无菌操作需要密切遵守操作规程和操作规范。

Sterile operation requires strict adherence to operation procedures and standards.
在进行无菌操作之前，要进行无菌操作台和周围区域的检查和清洁。

Before sterile operation, check and clean the sterile operating table and the surrounding area.
无菌操作需要操作者保持良好的操作习惯和卫生习惯。

Sterile operation requires operators to maintain good operating and hygiene habits.。