phosholipase C,epsilon1

• Genomic Location: Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 10q23 Ensembl cytogenetic band: 10q23.33 HGNC cytogenetic band: 10q23 • PLCE1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
Regulation of a Novel Human Phospholipase C, PLCe, through Membrane Targeting （膜靶向）by Ras* Received for publication, September 12, 2000, and in revised form, October 3, 2000 Published, JBC Papers in Press, October 5, 2000, DOI 10.1074/jbc.M008324200 Chunhua Song‡§, Chang-Deng Hu‡§, Misa Masago‡, Ken-ichi Kariya¶, Yuriko Yamawaki-Kataoka‡, Mitsushige Shibatohge‡, Dongmei Wu‡, Takaya Satoh‡, and Tohru Kataoka
UniProtKB/Swiss-Prot: summary for
PLCE1_HUMAN, Q9P212
Function: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation PLCE1: Phospholipases are a group of enzymes that hydrolyze phospholipids into fatty acids and other lipophilic molecules. PLC is subdivided into beta, gamma, delta, epsilon, zeta and eta subtypes, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol (DAG). IP3 and DAG both have important second messenger functions. PLC-beta is primarily activated by Gq/11 proteins and PLC-gamma is activated by phosphorylation in response to a variety of growth factor and immune system signals. Phospholipases are ubiquitously expressed and have diverse biological functions including roles in inflammation, cell growth, signaling and death and maintenance of membrane phospholipids.
Cellular Biology and 2Division of Molecular Pathology, Department of Biomedical Informatics, Kobe University Graduate School of Medicine, Kobe, Japan; and 3Department of Animal Genomics, Functional Genomics Institute, Mie University Life Science Research Center, Mie, Japan
Furthermore, the papillomas formed in PLCε-/- mice fail to undergo（经历） malignant （恶性的，有害的）progression（前 进，进展） into carcinomas （癌）, in contrast to（与。。。对照） a malignant conversion rate（转化率） of approximately （大约的）20% observed with papillomas（乳突淋瘤） in PLCε+/+ mice. In all of the tumors analyzed, the Ha-ras gene is Mutationally（转化，变形） activated irrespective of（不论，不管） the PLCε background. The skin of PLCε-/- mice fails to exhibit basal layer cell proliferation（细胞增 殖） and epidermal（表皮的，外皮的） Hyperplasia（增生） in response to TPA treatment. These results indicate （表明）a crucial role of PLCε in ras oncogene（致癌基因）-induced（引诱） de novo carcinogenesis and downstream signaling from TPA, introducing PLCε as a candidate （候选） molecular（分子的） target for the development of anticancer （抗癌的）drugs
resultsindicate表明acrucialrolerasoncogene致癌基因induced引诱denovocarcinogenesisdownstreamsignalingfromtpaintroducingplccandidate候选molecular分子的targetanticancer抗癌的drugsregulationnovelhumanphospholipaseplcethroughmembranetargeting膜靶向byrasreceivedpublicationseptember122000revisedformoctober2000publishedjbcpaperspressoctober2000doi101074jbcm008324200chunhuasong?changdenghu?misamasago?kenichikariyayurikoyamawakikataoka?mitsushigeshibatohge?dongmeiwu?takayasatoh?tohrukataokaphosphoinositidef?sf?uin?usitaid磷酸肌醇specific表示限定的特有的phospholipasepiplcplayspivotalrole关键作用inregulationintracellularintr?seljul?细胞内的signaltransduction转导fromvarious各种各样的receptor受体molecules分子
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Start:95,753,746 bp from pter End:96,088,149 bp from pter Size:334,404 bases Orientation:plus strand
Crucial Role of Phospholipase C in Chemical CarcinogenInduced Skin Tumor Development Yunfeng Bai,1 Hironori Edamatsu,1 Sakan Maeda,2 Hiromitsu Saito,3 Noboru Suzuki,3 Takaya Satoh,1 and Tohru Kataoka1 1Division of Molecular Biology,.Department of Molecular and
phospholipase C, epsilon 1
Entrez Gene summary for
PLCE1: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
பைடு நூலகம்
Mutational(突变的) activation of the ras proto-oncogenes （[‘prəutəu’ɔŋkədʒi:n] 原癌基因） is frequently found in skin cancers. However, the nature of downstream （[‘daun’stri:m] 下游的）signaling pathways from Ras involved in skin carcinogenesis （[,kɑ:sinəu‘dʒenisis] 致癌 作用）remains poorly understood Recently, we and others identified phospholipase C (PLC) E as an effector of Ras. Here we have examined the role of PLCE in de novo(重新 合成的) skin chemical carcinogenesis by using mice whose PLCε is genetically inactivated. PLCε-/- mice exhibit delayed onset and markedly（显著的） reduced incidence of skin squamous（鳞状的） tumors induced by initiation（[i,niʃi‘eiʃən] 启蒙的，开始的 ） with 7,12-dimethylbenz(a)anthracene followed by promotion（促进） with 12-O-tetradecanoylphorbol13Acetate（佛波醇12-十四酸酯13-乙酸酯 ） (TPA)..