Role of nestin in glioma invasion

Role of nestin in glioma invasion
Alex Lin;Luigi Marchionni;Jeffrey Sosnowski;David Berman;Charles G Eberhart;Eli E Bar
【期刊名称】《世界转化医学杂志》
【年(卷),期】2015(4)3
【摘要】AIM: To determine the role for the intermediate filament protein nestin in glioma invasion. METHODS: We examined the expression and function of nestin in gliomas(Grades Ⅱ-Ⅳ as defined by the World Health Organization). We determined nestin expression using Immunohistochemical methods. To elucidate nestin's biological function(s), we reduced m RNA levels by 61% and 87% in two glioblastomaderived neurosphere lines using short hairpin RNAs and determined the effect of reduced nestin expression on glioma cell proliferation and invasion using MTS and matrigel migration assays, respectively. We also utilized quantitative real time polymerase chain reaction assaysto determine the effect of reduced nestin expression on the expression of other markers associated with glioma stem cells and their differentiated progenies. RESULTS: We found a significant correlation between nestin immunoreactivity and astrocytoma tumor grade, with 36% of grade Ⅱ, 75% of grade Ⅲ, and 100% of grade Ⅳtumors expressing significant levels of
the protein when assessed using immunohistochemistry. Reduction in nestin expression had no effect on cell growth in culture, but did retard the capacity of one line to migrate in-vitro on matrigel. Interestingly, in the
line whose migration was not affected, m RNA levels of a second intermediate filament, synemin(also knowns as desmuslin), were elevated following introduction of sh RNA targeting nestin. As synemin was not induced in the line which required nestin for migration, it is a possibility that synemin may compensate for the loss of nestin in this process. CONCLUSION: Nestin expression is prominent in high-grade astrocytomas. Nestin is not required for cell growth but it may, however, be required for cell motility.
【总页数】10页(P78-87)
【关键词】Nestin;Stem;cells;Migration;Glioma;Neurosphere
【作者】Alex Lin;Luigi Marchionni;Jeffrey Sosnowski;David Berman;Charles G Eberhart;Eli E Bar
【作者单位】Department of Pathology, Johns Hopkins University School of Medicine;Department of Oncology, Johns Hopkins University School of Medicine;Department of Orthopedic Surgery, Johns Hopkins University School of Medicine;Department of Ophthalmology, Johns Hopkins University School of Medicine;Department of Neurological Surgery, School of Medicine, Case Western Reserve University;Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine
【正文语种】中文
【中图分类】R
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