高效液相色谱法测定麻黄及其制剂中的麻黄类生物碱和川芎嗪

高效液相色谱法测定麻黄及其制剂中的麻黄类生物碱和川芎嗪李红霞;丁明玉;吕琨;郁鉴源
【期刊名称】《色谱》
【年(卷),期】2001(019)002
【摘要】A sensitive and reliable high performance liquid chromatographic method(HPLC) has been developed for the first time for the simultaneous determination of the active ingredients of ephedrine alkaloids and 2,3,5,6-tetramethyl pyrazine (TMP) in Ephedra herba crude drug and two Chinese traditional medicines (Xiao-er qingfeiwan and Lu-si kewan)． The HPLC assay was performed on a reversed phase C18 column (Nova-Pak C18, 3．9 mm i.d.×150 mm) by using methanol-0．02 mol/L KH2PO4-acetic acid-triethyl amine (4∶96∶0．2∶0．01, V/V) as mobile phase for the ephedrine alkaloids analysis and methanol-H-2O-acetic acid
(35∶65∶0．5,V/V) as mobile phase for TMP analysis． Regression equations revealed the linear relationships (correlation coefficients: 0．991-0．998) between the peak area of each constituent (E, PE, NE, NPE, TMP) and its concentration． The detection limits for E, PE, NE, NPE and TMP were 0．4 mg/L, 0．1 mg/L, 0．03 mg/L, 0．02 mg/L and 0．03 mg/L, respectively, and the recoveries ranged between 92%-103%． The contents of E, PE, NE, NPE, TMP in Ephedra herba, traditional medicine Xiao-er qingfeiwan and Lu-si kewan were determined respectively． The relative standard deviations (RSD) of the contents ranged between 1．1%-
3%．%用RP-HPLC分离测定了麻黄及其制剂中的麻黄类生物碱及川芎嗪。

采用
色谱柱Nova-Pak C18(150 mm×3．9 mm i.d.),二极管阵列检测器（DAD）。

测定麻黄类生物碱时，以甲醇-0．02 mol/L KH2PO4-乙酸-三乙胺(体积比为
4∶96∶0．2∶0．01)为流动相，在210 nm波长下检测；测定川芎嗪时，以甲醇-水-乙酸(体积比为35∶65∶0．5) 为流动相, 在290 nm波长下检测。

分别测定了麻黄原药材、中成药(小儿清肺丸、鹭鸶咳丸)中麻黄碱、伪麻黄碱、去甲基麻黄碱、去甲基伪麻黄碱和川芎嗪的含量，检出限分别为0．4 mg/L,0．1 mg/L,0．03
mg/L,0．02 mg/L,0．03 mg/L。

该法准确、灵敏、快速。

【总页数】3页(P161-163)
【作者】李红霞;丁明玉;吕琨;郁鉴源
【作者单位】清华大学化学系,;清华大学化学系,;清华大学化学系,;清华大学化学系,【正文语种】中文
【中图分类】O658
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