eIF4E基因下调对人乳腺癌Bcap37细胞凋亡的影响

eIF4E基因下调对人乳腺癌Bcap37细胞凋亡的影响
林宇;许惠玉;邹宇;崔红霞;刘吉成
【期刊名称】《医学研究杂志》
【年(卷),期】2012(041)004
【摘要】目的应用化学合成的siRNA干扰乳腺癌Bcap37细胞真核细胞翻译起始因子4E( eukaryotic translation initiation factor 4E,eIF4E)的表达,探讨其对乳腺癌细胞凋亡和生长周期的影响.方法化学合成eIF4E基因的siRNA,转染人乳腺癌Bcap37细胞,采用Western blotting法及RT-PCR法检测转染前后Bcap37细胞中eIF4E蛋白和mRNA表达；应用流式细胞技术检测转染前后细胞周期的变化；AO/EB双染色法检测细胞凋亡；酶标仪检测caspase -3活性.结果 RT - PCR与Western blotting法检测结果显示,转染eIF4E -siRNA - 3#组细胞eIF4E基因的mRNA和蛋白表达均明显下降(P＜0.01)；eIF4E - siRNA转染组G1期细胞比例显著增加,S期细胞比例相对减少,差异显著(P＜0.05)；转染eIF4E - siRNA可诱导细胞凋亡,caspase-3活性亦增强.结论 eIF4E - siRNA可下调eIF4E基因在乳腺癌Bcap37细胞中的表达水平,诱导细胞凋亡.%Objective To investigate the effect of down - regulation of eIF4E on the spoptosig and eel! cycle of human breast cancer Bcap37 cells. Methods The siRNA of eIF4E by chemical systhesis was transfected into breast cancer Bcap37 cells. The expressions of eIF4E mRNA and protein were examined by RT - PCR and Western blotting. AO/EB doubly staining and microplate spectrophotometer were used to detect the apoptosis and activity of caspase - 3. Results The expression of eIF4E mRNA and protein were all decreased markedly after
transfection with eIF4E - siRNA - 3#. The results of cell cycle detection showed that the percentage of Bcap37 cells in the G, phase obviously increased, while the percentage of Bcap37 cells in the S phase significantly reduced(P<0. 05). It also can induce ap-optoais of cells and increase the activity of caspase - 3- Conclusion Transfection with eIF4E - siRNA - 3# can down - regulate elF4E gene expression in human breast cancer Bcap37 cells and induce apoptosis of cells.
【总页数】3页(P41-43)
【作者】林宇;许惠玉;邹宇;崔红霞;刘吉成
【作者单位】161006 齐齐哈尔医学院;161006 齐齐哈尔医学院;150040哈尔滨,黑龙江中医药大学;161006 齐齐哈尔医学院;161006 齐齐哈尔医学院
【正文语种】中文
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