Contribution of sigma meson pole to K_L-K_S mass difference
Linear sigma model at finite temperature
2 is the order of this transition, is it first–order with latent heat, or second–order, or maybe a crossover between the A. The potential at one–loop 27 phases? Lattice calculations suggest that when we consider two massless quarks, the transition is second order B. One loop mass correction 27 and the same suggest other approaches based on effective models. If we consider three massless flavours of quarks, C. The Keldysh contour 28 the transition is probably first–order [3, 6]. The aim of this work is to study the chiral phase transition. D. The pion self–energy graphs 28 1. The pion self–energy graph with one thermal pion28 Chiral symmetry breaking is a necessary ingredient for low energy hadron physics, since unbroken chiral symmetry results in massless baryons, without parity doubled 2. The pion
磁学 径向克尔 英文 kerr effect
IntroductionThe Kerr effect, also known as the magneto-optic Kerr effect (MOKE), is a phenomenon that manifests the interaction between light and magnetic fields in a material. It is named after its discoverer, John Kerr, who observed this effect in 1877. The radial Kerr effect, specifically, refers to the variation in polarization state of light upon reflection from a magnetized surface, where the change occurs radially with respect to the magnetization direction. This unique aspect of the Kerr effect has significant implications in various scientific disciplines, including condensed matter physics, materials science, and optoelectronics. This paper presents a comprehensive, multifaceted analysis of the radial Kerr effect, delving into its underlying principles, experimental techniques, applications, and ongoing research directions.I. Theoretical Foundations of the Radial Kerr EffectA. Basic PrinciplesThe radial Kerr effect arises due to the anisotropic nature of the refractive index of a ferromagnetic or ferrimagnetic material when subjected to an external magnetic field. When linearly polarized light impinges on such a magnetized surface, the reflected beam experiences a change in its polarization state, which is characterized by a rotation of the plane of polarization and/or a change in ellipticity. This alteration is radially dependent on the orientation of the magnetization vector relative to the incident light's plane of incidence. The radial Kerr effect is fundamentally governed by the Faraday-Kerr law, which describes the relationship between the change in polarization angle (ΔθK) and the applied magnetic field (H):ΔθK = nHKVwhere n is the sample's refractive index, H is the magnetic field strength, K is the Kerr constant, and V is the Verdet constant, which depends on the wavelength of the incident light and the magnetic properties of the material.B. Microscopic MechanismsAt the microscopic level, the radial Kerr effect can be attributed to twoprimary mechanisms: the spin-orbit interaction and the exchange interaction. The spin-orbit interaction arises from the coupling between the electron's spin and its orbital motion in the presence of an electric field gradient, leading to a magnetic-field-dependent modification of the electron density distribution and, consequently, the refractive index. The exchange interaction, on the other hand, influences the Kerr effect through its role in determining the magnetic structure and the alignment of magnetic moments within the material.C. Material DependenceThe magnitude and sign of the radial Kerr effect are highly dependent on the magnetic and optical properties of the material under investigation. Ferromagnetic and ferrimagnetic materials generally exhibit larger Kerr rotations due to their strong net magnetization. Additionally, the effect is sensitive to factors such as crystal structure, chemical composition, and doping levels, making it a valuable tool for studying the magnetic and electronic structure of complex materials.II. Experimental Techniques for Measuring the Radial Kerr EffectA. MOKE SetupA typical MOKE setup consists of a light source, polarizers, a magnetized sample, and a detector. In the case of radial Kerr measurements, the sample is usually magnetized along a radial direction, and the incident light is either p-polarized (electric field parallel to the plane of incidence) or s-polarized (electric field perpendicular to the plane of incidence). By monitoring the change in the polarization state of the reflected light as a function of the applied magnetic field, the radial Kerr effect can be quantified.B. Advanced MOKE TechniquesSeveral advanced MOKE techniques have been developed to enhance the sensitivity and specificity of radial Kerr effect measurements. These include polar MOKE, longitudinal MOKE, and polarizing neutron reflectometry, each tailored to probe different aspects of the magnetic structure and dynamics. Moreover, time-resolved MOKE setups enable the study of ultrafast magneticphenomena, such as spin dynamics and all-optical switching, by employing pulsed laser sources and high-speed detection systems.III. Applications of the Radial Kerr EffectA. Magnetic Domain Imaging and CharacterizationThe radial Kerr effect plays a crucial role in visualizing and analyzing magnetic domains in ferromagnetic and ferrimagnetic materials. By raster-scanning a focused laser beam over the sample surface while monitoring the Kerr signal, high-resolution maps of domain patterns, domain wall structures, and magnetic domain evolution can be obtained. This information is vital for understanding the fundamental mechanisms governing magnetic behavior and optimizing the performance of magnetic devices.B. Magnetometry and SensingDue to its sensitivity to both the magnitude and direction of the magnetic field, the radial Kerr effect finds applications in magnetometry and sensing technologies. MOKE-based sensors offer high spatial resolution, non-destructive testing capabilities, and compatibility with various sample geometries, making them suitable for applications ranging from magnetic storage media characterization to biomedical imaging.C. Spintronics and MagnonicsThe radial Kerr effect is instrumental in investigating spintronic and magnonic phenomena, where the manipulation and control of spin degrees of freedom in solids are exploited for novel device concepts. For instance, it can be used to study spin-wave propagation, spin-transfer torque effects, and all-optical magnetic switching, which are key elements in the development of spintronic memory, logic devices, and magnonic circuits.IV. Current Research Directions and Future PerspectivesA. Advanced Materials and NanostructuresOngoing research in the field focuses on exploring the radial Kerr effect in novel magnetic materials, such as multiferroics, topological magnets, and magnetic thin films and nanostructures. These studies aim to uncover newmagnetooptical phenomena, understand the interplay between magnetic, electric, and structural order parameters, and develop materials with tailored Kerr responses for next-generation optoelectronic and spintronic applications.B. Ultrafast Magnetism and Spin DynamicsThe advent of femtosecond laser technology has enabled researchers to investigate the radial Kerr effect on ultrafast timescales, revealing fascinating insights into the fundamental processes governing magnetic relaxation, spin precession, and all-optical manipulation of magnetic order. Future work in this area promises to deepen our understanding of ultrafast magnetism and pave the way for the development of ultrafast magnetic switches and memories.C. Quantum Information ProcessingRecent studies have demonstrated the potential of the radial Kerr effect in quantum information processing applications. For example, the manipulation of single spins in solid-state systems using the radial Kerr effect could lead to the realization of scalable, robust quantum bits (qubits) and quantum communication protocols. Further exploration in this direction may open up new avenues for quantum computing and cryptography.ConclusionThe radial Kerr effect, a manifestation of the intricate interplay between light and magnetism, offers a powerful and versatile platform for probing the magnetic properties and dynamics of materials. Its profound impact on various scientific disciplines, coupled with ongoing advancements in experimental techniques and materials engineering, underscores the continued importance of this phenomenon in shaping our understanding of magnetism and driving technological innovations in optoelectronics, spintronics, and quantum information processing. As research in these fields progresses, the radial Kerr effect will undoubtedly continue to serve as a cornerstone for unraveling the mysteries of magnetic materials and harnessing their potential for transformative technologies.。
Atg8, a ubiquitin-like protein required for autophagosome formation
Atg8,a Ubiquitin-like Protein Required for Autophagosome Formation,Mediates Membrane Tethering and HemifusionHitoshi Nakatogawa,1,2Yoshinobu Ichimura,1,3and Yoshinori Ohsumi1,*1Department of Cell Biology,National Institute for Basic Biology,Okazaki444-8585,Japan2PRESTO,Japan Science and Technology Agency,Saitama332-0012,Japan3Present address:Department of Biochemistry,Juntendo University School of Medicine,Bunkyo-ku,Tokyo113-8421,Japan. *Correspondence:yohsumi@nibb.ac.jpDOI10.1016/j.cell.2007.05.021SUMMARYAutophagy involves de novo formation of double membrane-bound structures called autophagosomes,which engulf material to be degraded in lytic compartments.Atg8is a ubiq-uitin-like protein required for this process in Saccharomyces cerevisiae that can be conju-gated to the lipid phosphatidylethanolamine by a ubiquitin-like system.Here,we show using an in vitro system that Atg8mediates the teth-ering and hemifusion of membranes,which are evoked by the lipidation of the protein and reversibly modulated by the deconjugation enzyme Atg4.Mutational analyses suggest that membrane tethering and hemifusion ob-served in vitro represent an authentic function of Atg8in autophagosome formation in vivo.In addition,electron microscopic analyses indicate that these functions of Atg8are in-volved in the expansion of autophagosomal membranes.Our results provide further insights into the mechanisms underlying the unique membrane dynamics of autophagy and also in-dicate the functional versatility of ubiquitin-like proteins.INTRODUCTIONAutophagy is an evolutionally conserved protein degrada-tion pathway in eukaryotes that is essential for cell survival under nutrient-limiting conditions(Levine and Klionsky, 2004).In addition,recent studies have revealed a wide variety of physiological roles for autophagy(Mizushima, 2005)as well as its relevance to diseases(Cuervo,2004). During autophagy,cup-shaped,single membrane-bound structures called isolation membranes appear and expand,which results in the sequestration of a portion of the cytosol and often organelles.Eventually,spherical, double membrane-bound structures called autophago-somes are formed(Baba et al.,1994),and then delivered to and fused with lysosomes or vacuoles to allow their contents to be degraded.Studies in S.cerevisiae have identified18ATG genes required for autophagosome formation,most of which are also found in higher eukary-otes(Levine and Klionsky,2004).Recent studies have shown that Atg proteins constitutefive functional groups: (i)the Atg1protein kinase complex,(ii)the Atg14-contain-ing phosphatidylinositol-3kinase complex,(iii)the Atg12-Atg5protein conjugation system,(iv)the Atg8lipid con-jugation system,and(v)the Atg9membrane protein recycling system(Yorimitsu and Klionsky,2005).The mechanisms by which these units act collaboratively with lipid molecules to form the autophagosomes,how-ever,are still poorly understood.Atg8is one of two ubiquitin-like proteins required for autophagosome formation(Mizushima et al.,1998;Ichi-mura et al.,2000).Because it has been shown that Atg8 and its homologs(LC3in mammals)localize on the isola-tion membranes and the autophagosomes,these proteins have been used in various studies as reliable markers for the induction and progression of autophagy(Kirisako et al.,1999;Kabeya et al.,2000;Yoshimoto et al.,2004). In S.cerevisiae,Atg8is synthesized with an arginine resi-due at the C terminus,which is immediately removed by the cysteine protease Atg4(Kirisako et al.,2000).The resulting Atg8G116protein has a glycine residue at the new C terminus and can serve as substrate in a ubiqui-tin-like conjugation reaction catalyzed by Atg7and Atg3, which correspond to the E1and E2enzymes of the ubiq-uitination system,respectively(Ichimura et al.,2000). Remarkably,unlike other ubiquitin-like conjugation sys-tems,Atg8is conjugated to the lipid phosphatidylethanol-amine(PE),thereby Atg8is anchored to membranes (Ichimura et al.,2000;Kirisako et al.,2000).Immunoelec-tron microscopy revealed that Atg8,probably as a PE-conjugated form(Atg8-PE),is predominantly localized on the isolation membranes rather than on the complete autophagosomes(Kirisako et al.,1999),suggesting that Atg8-PE plays a pivotal role in the process of autophago-some formation.The precise function of Atg8-PE,how-ever,has remained unknown.The conjugation of Atg8to PE is reversible;Atg4also functions as a deconjugation enzyme,resulting in the Cell130,165–178,July13,2007ª2007Elsevier Inc.165release of Atg8from the membrane(Kirisako et al.,2000). This reaction is thought to be important for the regulation of the function of Atg8and/or the recycling of Atg8after it has fulfilled its role in autophagosome formation.We reconstituted the Atg8-PE conjugation reaction in vitro with purified components(Ichimura et al.,2004). Here,we show using this system that Atg8mediates the tethering and hemifusion of liposomes in response to the conjugation with PE.These phenomena observed in vitro are suggested to reflect a bonafide in vivo function of Atg8 in the expansion of the isolation membrane.Based on mutational analyses and structural information,the mech-anisms of Atg8-mediated membrane tethering and hemi-fusion as well as its regulation are discussed.This study sheds light on the molecular basis of unconventional membrane dynamics during autophagy,which is gov-erned by the Atg proteins.RESULTSLipidation of Atg8Causes Clustering of LiposomesIn VitroAs reported previously(Ichimura et al.,2004),when puri-fied Atg8G116(hereafter,referred to as Atg8),Atg7,and Atg3were incubated with liposomes containing PE in the presence of ATP,Atg8-PE was efficiently formed (Figure1A,lanes1–6).Intriguingly,the reaction mixture became turbid during the incubation(Figure1B),which under a light microscope,was found to be a result of grad-ually forming aggregates(Figure1C).Both the degree of turbidity and the size of the aggregates appeared to corre-late with the amount of Atg8-PE produced in the mixture. Size-distribution analyses using dynamic light scattering (DLS)clearly showed that the aggregates formed in an Atg8-PE dose-dependent manner(Figure1D).These aggregates disappeared when the samples were treated with the detergent CHAPS(Figure1E,+CHAPS).In addi-tion,if a small amount of PE modified with thefluorescent dye7-nitro-2,1,3-benzoxadiazol-4-yl(NBD)was included in the liposome preparation,the aggregates became uniformlyfluorescent(Figure1E,NBD-PE).These results suggest that the aggregates generated during the produc-tion of Atg8-PE were clusters of liposomes.When the proteins were denatured with urea,the clus-ters of liposomes dissociated,although Atg8remained conjugated to PE(Figure1E,+urea and Figure1F,lane 2),indicating that the liposomes aggregated due to some function of the Atg8protein rather than an artifact caused by Atg8-PE as the lipid with the extraordinarily large head group.When the aggregates were sedimented by centrifugation,Atg8-PE co-precipitated with the lipo-somes(Figure1G,lane2),whereas Atg7,Atg3,and unconjugated Atg8did not(Figure1G,lane3).The sedimented liposomes containing Atg8-PE remained clustered even if they were briefly sonicated(Figure1H, ppt.).These results suggested that Atg8-PE molecules function to tether together membranes to which they are anchored.Atg8-PE Also Mediates Liposome FusionWe also examined if membrane fusion occurred between the liposomes connected by Atg8-PE.To this end,we took advantage of a well-characterized lipid mixing assay (Struck et al.,1981).This method is based on energy transfer from NBD to lissamine rhodamine B(Rho),each of which is conjugated to PE.Because the amino group of the ethanolamine moiety is modified with the dyes, these lipids cannot be conjugated with Atg8.If both of the conjugated dyes are present at appropriate concen-trations in the same liposome,thefluorescence of NBD is effectively quenched by Rho(Figure2A,compare col-umns1and4).If a‘‘NBD+Rho’’liposome is fused with a‘‘nonlabeled’’liposome,which results in an increase of the average distance between the two dyes on the membrane,the NBDfluorescence will be dequenched.A mixture of the nonlabeled and NBD+Rho liposomes were subjected to the conjugation reaction.The resulting liposome clusters were dissociated by proteinase K treat-ment,followed byfluorescence measurements.Remark-ably,a significant ATP-dependent increase of thefluores-cence was observed(ATP is required for the production of Atg8-PE;Figure2B,column6).This increasedfluores-cence was not observed with samples of nonlabeled lipo-somes alone,NBD+Rho liposomes alone,or a mixture of nonlabeled liposomes and liposomes containing NBD-PE but not Rho-PE(Figure2B,columns1-3).These results suggest that membrane fusion occurred between the lipo-somes tethered together by Atg8-PE.The increasedfluo-rescence was only observed if the reaction mixture was treated with proteinase K(Figure2B,columns4and6). This appeared to be due to the presence of Atg7and/or Atg3rather than Atg8or some effect of the clustering, because the NBDfluorescence was not increased by the addition of Atg4(Figure2B,column5),which detached Atg8from the membranes and dissociated the clusters of liposomes(see below).Instead,decreasing the con-centrations of the conjugation enzymes allowed the dequenching of the NBDfluorescence to be detected without proteinase K digestion(Figure2B,column7). The fusion of the liposomes was examined with various amounts of Atg8(Figure2C).The level of fusion increased Atg8dose-dependently and reached maximum at2m M (Figure2C).In contrast,a larger amount of Atg8produced an inhibitory effect(data not shown).This suggested that formation of the large aggregates resulted from excessive tethering by Atg8-PE,which no longer lead to fusion. We also carried out time-course experiments to roughly estimate the fusion rate using the lower concentrations of the conjugation enzymes(Figure2D),which eliminated the need for the proteinase K treatment(Figure2B).It should be noted that the incubation time includes the times re-quired for the formation of Atg8-PE and the subsequent tethering and fusion reactions.Under these conditions, the band of Atg8-PE could be seen on an SDS-PAGE gel after a10min incubation,and the reaction was completed within30min(Figure S1in the Supplemental Data available with this article online).It appeared that166Cell130,165–178,July13,2007ª2007Elsevier Inc.Figure1.Membrane Tethering Function of Atg8-PE In Vitro(A–C)Purified Atg8(10m M),Atg7(1m M),and Atg3(1m M)were incubated with liposomes(350m M lipids)composed of55mol%DOPE,30mol% POPC,and15mol%blPI in the presence(lanes1–6)or absence(lanes7–12)of1mM ATP at30 C for the indicated time periods,followed by urea-SDS-PAGE and CBB-staining(A),measurement of the absorbance at600nm(B),or observation under a light microscope(Nomarski images)(C).(D)Conjugation reactions with the various amounts of Atg8were performed as described in(A).After incubation for60min,the size distribution of the aggregates was examined using DLS measurements.d.nm,apparent diameter(nm).(E and F)The conjugation reactions were carried out as described in(A).They were further incubated at30 C for30min in the presence of either 6M urea or1%CHAPS and were then subjected to microscopy(E)or urea-SDS-PAGE and CBB-staining(F).The reaction was also performed with liposomes containing1mol%NBD-labeled DOPE(thus containing54mol%unlabeled DOPE),followed byfluorescence microscopy.Afluo-rescence image with afilter for YFP(NBD-PE,FL)and a Nomarski image(NBD-PE,DIC)are shown.(G and H)Atg8(30m M),Atg7(2m M),and Atg3(2m M)were incubated with liposomes(350m M lipids)consisting of70mol%DOPE and30mol% POPC in the presence of1mM ATP at30 C for45min(total).The mixture was microcentrifuged at15,000rpm for10min to generate the pellet (ppt.)and the supernatant(sup.)fractions.The fractions were briefly sonicated and were analyzed by urea-SDS-PAGE(G)or observed under a light microscope(H).In this experiment,blPI was omitted to prevent Atg7and Atg3from tightly binding to the liposome.We showed that Atg8could also cause hemifusion of liposomes with this lipid composition.Cell130,165–178,July13,2007ª2007Elsevier Inc.167the liposomes began to fuse shortly after the formation of Atg8-PE.The fusion reaction proceeded concurrently with the conjugation reaction and continued for 30min after the completion of the Atg8-PE production (Figure 2D,filled circles).Small liposomes <100nm in diameter tend to sponta-neously fuse (Chen et al.,2006),and the liposomes we used in the above experiments were 70nm in diameter (Figure 1D).However,we also showed that Atg8-PEcaused a significant level of fusion between larger lipo-somes in spite of their stability against spontaneous fusion (Figure S1).Taken together,these results suggest that not only tethering but also fusion of the liposomes is mediated by Atg8-PE.The Atg8-Mediated Membrane Fusion Is Hemifusion Recent in vitro studies on membrane fusion mediated by SNARE proteins and a class of viral proteinsrevealedFigure 2.Membrane Hemifusion Occurs between Liposomes Tethered by Atg8-PE(A and B)Nonlabeled (55mol%DOPE,30mol%POPC,and 15mol%blPI),NBD-labeled (55mol%DOPE,29mol%POPC,15mol%blPI,and 1mol%NBD-DOPE),and NBD+Rho-labeled (55mol%DOPE,27.5mol%POPC,15mol%blPI,1mol%NBD-DOPE,and 1.5mol%Rho-DOPE)liposomes were mixed in the differ-ent combinations and ratios indicated.Their relative intensities of the NBD fluorescence ob-served are shown (the value obtained with a 4:1mixture of the nonlabeled and NBD+Rho lipo-somes was defined as 1)(A).These mixtures of liposomes were incubated with Atg8(4m M),Atg7(0.5or 1.0m M),and Atg3(0.5or 1.0m M)in the presence (filled columns)or absence (open columns)of 1mM ATP for 60min,and were then treated with 1unit/ml apyrase.The mixtures were further incubated for 30min with the buffer (columns 4and 7),1m M Atg4(columns 5and 8),or 0.2mg/ml proteinase K (columns 1-3,6and 9),followed by measure-ment of the NBD fluorescence.The experi-ments were repeated three times and the average fluorescence values divided by those obtained from the original liposome samples (F/F 0)are presented with error bars for the stan-dard deviations (B).(C)A 4:1mixture of the nonlabeled and NBD+Rho liposomes was incubated with various amounts of Atg8,1.0m M Atg7,and 1.0m M Atg3in the presence (open circles)or absence (filled circles)of ATP,and the samples were then treated with proteinase K,followed by measuring the NBD fluorescence.(D)The conjugation reactions were performed with the mixed liposomes used for the lipid mixing assay,0.5m M Atg7,and 0.5m M Atg3in the presence or absence of Atg8(4m M)and ATP.After incubation for the indicated time periods,an aliquot of the samples was immediately subjected to the fluorescence measurements.The values that were obtained by subtracting the signals observed in the absence of ATP from those observed in the presence of ATP are presented.(E)The lipid mixing assay was performed with 4m M Atg8,1m M Atg7,and 1m M Atg3in the presence or absence of ATP (white bars in columns 3and 2,respectively)as described in(C).For PEG-induced fusion reactions,the mixed liposomes were incubated at 37C for 30min in the presence or absence of 12.5%PEG 3350(white bars in columns 5and 4,respectively).These samples as well as the original liposomes (column 1)were then incubated with 20mM sodium dithionite on ice for 20min in the presence (black bars)or absence (gray bars)of 0.5%Triton X-100,followed by the NBD fluorescence measurement.168Cell 130,165–178,July 13,2007ª2007Elsevier Inc.that fusion proceeds through an intermediate state called hemifusion,in which outer(contacting)leaflets of two apposed lipid bilayers merge,while inner(distal)leaflets remain intact(Chernomordik and Kozlov,2005).It was also reported that fusion can be arrested or delayed at the hemifusion state under some conditions.Therefore, we investigated whether the liposome fusion caused by Atg8in vitro was complete fusion(the merger of both inner and outer leaflets)or hemifusion(Figure2E).This can be examined using the membrane impermeable reductant sodium dithionite that selectively abolishes thefluores-cence of NBD conjugated to the lipid head group in the outer leaflet(Meers et al.,2000).Accordingly,when so-dium dithionite was added to the original liposomes,the background level of the NBDfluorescence was decreased by about50%,whereas it was hardly detected in the pres-ence of the detergent(Figure2E,column1).Strikingly,the NBDfluorescence increased by the Atg8-mediated fusion was totally eliminated by addition of sodium dithionite to the same level as those observed in the original lipo-somes and the reaction mixture incubated without ATP (Figure2F,columns1-3).Whereas,we confirmed that in liposome fusion induced by polyethylene glycol(PEG), which causes complete fusion(Akiyama and Ito,2003), about half of the increasedfluorescence was retained af-ter sodium dithionite treatment(Figure2F,columns4and 5).Taken together,membrane fusion mediated by Atg8 in vitro was suggested to be hemifusion.To obtain direct evidence of hemifusion,we analyzed the morphology of liposomes by electron microscopy (Figures3A–3E),in which liposomal membranes were observed as double white lines that correspond to the outer and inner leaflets.When the clusters of liposomes formed by Atg8-PE were analyzed,tight junctions between the liposomes were observed(Figures3B and 3C,arrowheads).Consistent with the biochemical results suggesting that complete fusion does not occur,the size of the individual liposomes did not appear to significantly increase(compare Figures3A and3B).Instead,hallmarks of hemifusion,trifurcated structures formed by one contin-uous outer leaflet and two separate inner leaflets,could be observed at the junction between the liposomes(Figures 3C–3E,arrows).These results strongly support our con-clusion that Atg8-PE causes hemifusion of liposomes. Atg8Forms a Multimer in Responseto the Conjugation with PEWe also performed immunoelectron microscopy of the liposomes clustered by Atg8-PE(Figures3F–3I).Intrigu-ingly,Atg8-PE tended to be enriched at the junction be-tween the liposomes(Figures3G–3J).While,if the mixture incubated without ATP was similarly analyzed,the signal was rarely observed on the liposome(Figure3F;the gold particles observed should represent unconjugated Atg8 adsorbed onto the grid).These results indicate that Atg8-PE is directly involved in the tethering and hemifu-sion of liposomes.We observed that‘‘naked’’liposomes do not associate with liposomes carrying Atg8-PE(data not shown), suggesting that tethering should be achieved due to interactions between Atg8-PE molecules on different membranes.We therefore examined the intermolecular interaction of Atg8-PE by crosslinking experiments(Fig-ure4).The reaction mixture containing Atg8-PE or unconjugated Atg8was incubated with the lysine-to-lysine reactive crosslinker DSS.We found that a crosslink adduct with a molecular weight of 24kDa on a SDS-PAGE gel specifically appeared in the sample containing Atg8-PE(Figure4A,lane5).Considering the molecular weights of the proteins included,this adduct should represent an Atg8-PE homodimer.Immunoblotting anal-yses with anti-Atg8revealed that two additional crosslink adducts of about37and100kDa were also specifically produced in the Atg8-PE-containing sample(Figure4B, lanes2–4and Figure S2).These products were immuno-stained neither with anti-Atg7nor anti-Atg3(data not shown),suggesting that they represent a trimer and a larger multimer of Atg8-PE,respectively,and thus that Atg8multimerizes in response to PE conjugation. We also showed that this multimerization correlates with the membrane tethering ability of Atg8(see below), indicating that interactions between Atg8-PE molecules on different membranes are responsible for the tethering of the membranes.The Membrane-Tethering and HemifusionFunctions of Atg8Are Modulatedby the Deconjugation Enzyme Atg4Our results suggest that the membrane-tethering and hemifusion functions of Atg8are evoked by the conjuga-tion with PE,whereas Atg4functions as a deconjugase that cleaves the linkage between Atg8and PE(Kirisako et al.,2000).We reconstituted this reaction in vitro.After producing Atg8-PE using the conjugation reaction,the re-action was terminated by adding apyrase to deplete the remaining ATP.When purified Atg4was then added, Atg8-PE was rapidly and almost completely deconjugated (Figure4C,lanes1-6).In contrast,when the Atg4was pretreated with the cysteine protease inhibitor N-ethylma-leimide(NEM),the deconjugation reaction did not occur (Figure4C,lanes7–12).These results clearly show that Atg4is sufficient for the deconjugation of Atg8-PE.Upon deconjugation,the liposome aggregates immediately dissociated(Figure4D).In addition,we found that multi-merization of Atg8is also reversible;the crosslink adducts corresponding to the Atg8-PE dimer(Figure4A,lane6)as well as the trimer and the multimer(data not shown)were hardly formed when DSS was added after the deconjuga-tion reaction.We also showed that the presence of Atg4in the conjugation reaction retarded the accumulation of Atg8-PE and accordingly interfered with the tethering and hemifusion of the liposomes(data not shown).It was indicated that membrane tethering and hemifusion by Atg8can be regulated by the balance between the conjugation and deconjugation reactions.Cell130,165–178,July13,2007ª2007Elsevier Inc.169Identification of Mutations that Impair the Postconjugational Function of Atg8In VivoIf the function of Atg8-PE we observed in vitro was involved in autophagosome formation in vivo,Atg8mutants defi-cient for this function should result in defective autophagy.To examine this idea,we performed structure-based and systematic mutational analyses of Atg8(Figure 5).The structures of mammalian homologs revealed that Atg8family proteins consist of two domains:an N-terminal heli-cal domain (NHD)and a C-terminal ubiquitin-like domain (ULD)(Paz et al.,2000;Coyle et al.,2002;Sugawara et al.,2004;Figures 5E–5H).Among the highly conserved residues in the ULD,we selected those with side chains that were exposed on the domain surface (Figure 5A),and individually replaced them with alanine,except that serine was substituted for Ala75.Consequently,we didnot mutate residues suggested to be important for interac-tions with the conjugation enzymes,because these resi-dues are conserved only for their hydrophobic nature (Sugawara et al.,2004).The Atg8variants were expressed from centromeric plasmids in D atg8yeast cells,and their autophagic activities were biochemically assessed (see Supplemental Experimental Procedures ).In nutrient-rich media,the autophagic activity was low in all of the mutant cells as well as in the wild-type cells (data not shown).In contrast,in nitrogen starvation conditions,which strongly induced autophagy,a number of mutants were found to have defective autophagic phenotypes (Figure 5B).Ala-nine replacement of seven residues,Ile32,Lys48,Leu50,Arg65,Asp102,Phe104,and Tyr106,significantly impaired the autophagic activity to 30%–60%of that of the wild-type (Figure 5B).Immunoblotting analyses showedthatFigure 3.Electron Microscopic Analyses of the Liposomes Tethered and Hemi-fused by Atg8-PEConjugation reactions were performed with 4m M Atg8,1m M Atg7,and 1m M Atg3in the presence (B–E and G–I)or absence (A and F)of ATP for 60min and subjected to phospho-tungstic acid-staining and electron micros-copy (A–E).The junctions between the lipo-somes and the structures suggested to represent hemifusion are indicated with arrow-heads and arrows,respectively.The same samples were also subjected to immunostain-ing using purified anti-Atg8-IN-13and anti-rab-bit IgG conjugated with 5nm gold particles,fol-lowed by phosphotungstic acid-staining and electron microscopic observation (F–I).To as-sess the enrichment of Atg8-PE at the junction of the liposomes (J),images of two contacting liposomes as shown in G and H were randomly picked up (n =41).The lengths of contacting (CR)and noncontacting regions (non-CR)of the liposomal membranes were measured (white bars),thereby the number of gold parti-cles on each region (gray bars)was divided,in which the length of the contacting region was doubled,to calculate the linear density (black bars).The average values are presented with error bars for the standard deviations.170Cell 130,165–178,July 13,2007ª2007Elsevier Inc.a substantial amount of each of the Atg8mutant proteins accumulated in the cells (Figure 5D),although there were some differences in their mobilities in SDS-PAGE analysis;for instance the PE-conjugated and unconjugated forms of the D102A mutant exhibited almost the same mobility.None of the mutations significantly affected the formation of Atg8-PE (Figure 5D),suggesting that the mutations impaired a function of Atg8that was exerted after the conjugation with PE.Notably,these mutants accumulated different levels of unconjugated Atg8under the starvation conditions (Figure 5D,starvation),which allowed us to classify them into three groups.For the class I mutants K48A and L50A,the levels of the unconjugated forms were similar to that of the wild-type (Figure 5D,denoted in purple).On the other hand,compared to the wild-type,lower levels of the unconjugated forms were detected in the class II mutants I32A,D102A,F104A and Y106A (Figure 5D,denoted in red),whereas a larger amount of the unconju-gated class III mutant R65A accumulated (Figure 5D,denoted in orange).We then mapped the mutated resi-dues onto the three-dimensional structure of LC3(Suga-wara et al.,2004),which revealed that class of the mutant corresponded to the location of the mutation.All the class II residues were clustered in a specific region on the ULD (hereafter,referred to as the class II region),and the two neighboring class I residues were located close to the class II region (Figure 5E).In contrast,the class III residue was located away from the other mutated residues (Fig-ures 5G and 5H).The NHD of Atg8contains two helices:a 1and a 2(Figure 5A).We constructed two mutants,one with a dele-tion of a 1(D N8)and a second bearing deletions of both helices (D N24).It was shown that the NHD is involved in autophagy partially but significantly;the D N8and D N24mutations decreased the autophagic activity by about 30and 40%,respectively (Figure 5C).We also showed that the deletions did not affect the stability of the proteins or the formation of the PE conjugates (Figure S3).Effects of the Atg8Mutations on the Membrane-Tethering FunctionWe next examined whether the mutations affected the liposome-clustering ability of Atg8in vitro (Figure 6).Figure 4.The Membrane-Tethering Function and Multimerization of Atg8Are Reversibly Regulated in Response to Conjugation with PE(A)Conjugation reactions were performed as described in Figure 3in the presence (lanes 2,3,5,and 6)or absence (lanes 1and 4)of ATP.They were mixed with 1unit/ml apyrase,and then incubated with (lanes 3and 6)or with-out (lanes 1,2,4,and 5)purified Atg4(0.5m M)at 30 C for 30min.These samples were further incubated with (lanes 4–6)or without (lanes 1–3)100m M DSS for 30min,and then analyzed by urea-SDS-PAGE and CBB-staining.(B)The reaction mixture including ATP was in-cubated with different concentrations of DSS as indicated,followed by urea-SDS-PAGE and immunoblotting with anti-Atg8-IN13.We also identified a crosslink product that reacted with anti-Atg3(Atg8xAtg3).(C and D)The conjugation reactions performed as described in Figure 1A were mixed with 1unit/ml apyrase.Atg4(0.5m M)pretreated with (lanes 7–12)or without (lanes 1–6)10mM NEM was then added,and the samples were incubated for the indicated time periods and subjected to urea-SDS-PAGE and CBB-stain-ing (C).The same samples were also observed under a light microscope (D).Cell 130,165–178,July 13,2007ª2007Elsevier Inc.171。
第五章 核磁共振波谱分析 (nmr)
核磁共振基本原理
原子核的磁矩 原子核是带正电荷的粒子,和电子一样有自旋现象, 因而具有自旋角动量以及相应的自旋量子数。
p为角动量,其值是量子化的,可用自旋量子 数表示
式中:h为普郎克常数(6.63×10−34J· s); −I为自旋量子数,与原子的质量数及原子序数 有关。
由于原子核是具有一定质量的带正电的粒子,故在 自旋时会产生核磁矩。核磁矩和角动量都是矢量, 它们的方向相互平行,且磁矩与角动量成正比,即 μ=γp 式中:γ为旋磁比,即核磁矩与核的自旋角动量的 比值,不同的核具有不同旋磁比,它是磁核的一个 特征值;μ为磁矩,用核磁子表示,1核磁子单位等 于5.05×10−27J· T−1;
原子核的自旋形状
有机化合物的基本元素13C、1H、15N、19F、31P 等都有核磁共振信号,且自旋量子数均为1/2,核磁共振 信号相对简单,已广泛用于有机化合物的结构测定 然而,核磁共振信号的强弱是与被测磁性核的天然 丰度和旋磁比的立方成正比的,如1H的天然丰度为 99.985%,19F和31P的丰度均为100%,因此,它们的 共振信号较强,容易测定,而13C的天然丰度只有1.1%, 很有用的15N和17O核的丰度也在1%以下,它们的共振信 号都很弱,必须在傅里叶变换核磁共振波谱仪上经过多次 扫描才能得到有用的信息。
1H、 19F、 31P
当I=0时,p=0,原子核没有磁矩,没有自旋现象;当I >0时,p≠ 0,原子核磁矩不为零,有自旋现象。 I=1/2的原子核在自旋过程中核外电子云呈均匀的球 型分布,见图(b)核磁共振谱线较窄,最适宜核磁共 振检测,是NMR主要的研究对象。I>1/2的原子核, 自旋过程中电荷在核表面非均匀分布
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第 4卷 第 5 0 期
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The decay $rho^{0}to pi^{+}+pi^{-}+gamma$ and the coupling constant g$_{rhosigmagamma}$
a rXiv:n ucl-t h /441v28Ma y2The decay ρ0→π++π−+γand the coupling constant g ρσγA.Gokalp ∗and O.Yilmaz †Physics Department,Middle East Technical University,06531Ankara,Turkey(February 8,2008)Abstract The experimental branching ratio for the radiative decay ρ0→π++π−+γis used to estimate the coupling constant g ρσγfor a set of values of σ-meson parameters M σand Γσ.Our results are quite different than the values of this constant used in the literature.PACS numbers:12.20.Ds,13.40.HqTypeset using REVT E XThe radiative decay processρ0→π++π−+γhas been studied employing different approaches[1,5].There are two mechanisms that can contribute to this radiative decay: thefirst one is the internal bremsstrahlung where one of the charged pions from the decay ρ0→π++π−emits a photon,and the second one is the structural radiation which is caused by the internal transformation of theρ-meson quark structure.Since the bremsstrahlung is well described by quantum electrodynamics,different methods have been used to estimate the contribution of the structural radiation.Singer[1]calculated the amplitude for this decay by considering only the bremsstrahlung mechanism since the decayρ0→π++π−is the main decay mode ofρ0-meson.He also used the universality of the coupling of theρ-meson to pions and nucleons to determine the coupling constant gρππfrom the knowledge of the coupling constant gρter,Renard [3]studied this decay among other vector meson decays into2π+γfinal states in a gauge invariant way with current algebra,hard-pion and Ward-identities techniques.He,moreover, established the correspondence between these current algebra results and the structure of the amplitude calculated in the single particle approximation for the intermediate states.In corresponding Feynman diagrams the structural radiation proceeds through the intermediate states asρ0→S+γwhere the meson S subsequently decays into aπ+π−pair.He concluded that the leading term is the pion bremsstrahlung and that the largest contribution to the structural radiation amplitude results from the scalarσ-meson intermediate state.He used the rough estimate gρσγ≃1for the coupling constant gρσγwhich was obtained with the spin independence assumption in the quark model.The coupling constant gρππwas determined using the then available experimental decay rate ofρ-meson and also current algebra results as3.2≤gρππ≤4.9.On the other hand,the coupling constant gσππwas deduced from the assumed decay rateΓ≃100MeV for theσ-meson as gσππ=3.4with Mσ=400MeV. Furthermore,he observed that theσ-contribution modifies the shape of the photon spectrum for high momenta differently depending on the mass of theσ-meson.We like to note, however,that the nature of theσ-meson as a¯q q state in the naive quark model and therefore the estimation of the coupling constant gρσγin the quark model have been a subject ofcontroversy.Indeed,Jaffe[6,7]lately argued within the framework of lattice QCD calculation of pseudoscalar meson scattering amplitudes that the light scalar mesons are¯q2q2states rather than¯q q states.Recently,on the other hand,the coupling constant gρσγhas become an important input for the studies ofρ0-meson photoproduction on nucleons.The presently available data[8] on the photoproduction ofρ0-meson on proton targets near threshold can be described at low momentum transfers by a simple one-meson exchange model[9].Friman and Soyeur [9]showed that in this picture theρ0-meson photoproduction cross section on protons is given mainly byσ-exchange.They calculated theγσρ-vertex assuming Vector Dominance of the electromagnetic current,and their result when derived using an effective Lagrangian for theγσρ-vertex gives the value gρσγ≃2.71for this coupling ter,Titov et al.[10]in their study of the structure of theφ-meson photoproduction amplitude based on one-meson exchange and Pomeron-exchange mechanisms used the coupling constant gφσγwhich they calculated from the above value of gρσγinvoking unitary symmetry arguments as gφσγ≃0.047.They concluded that the data at low energies near threshold can accommodate either the second Pomeron or the scalar mesons exchange,and the differences between these competing mechanisms have profound effects on the cross sections and the polarization observables.It,therefore,appears of much interest to study the coupling constant gρσγthat plays an important role in scalar meson exchange mechanism from a different perspective other than Vector Meson Dominance as well.For this purpose we calculate the branching ratio for the radiative decayρ0→π++π−+γ,and using the experimental value0.0099±0.0016for this branching ratio[11],we estimate the coupling constant gρσγ.Our calculation is based on the Feynman diagrams shown in Fig.1.Thefirst two terms in thisfigure are not gauge invariant and they are supplemented by the direct term shown in Fig.1(c)to establish gauge invariance.Guided by Renard’s[3]current algebra results,we assume that the structural radiation amplitude is dominated byσ-meson intermediate state which is depicted in Fig. 1(d).We describe theρσγ-vertex by the effective LagrangianL int.ρσγ=e4πMρMρ)2 3/2.(3)The experimental value of the widthΓ=151MeV[11]then yields the value g2ρππ2gσππMσ π· πσ.(4) The decay width of theσ-meson that follows from this effective Lagrangian is given asΓσ≡Γ(σ→ππ)=g2σππ8 1−(2Mπ2iΓσ,whereΓσisgiven by Eq.(5).Since the experimental candidate forσ-meson f0(400-1200)has a width (600-1000)MeV[11],we obtain a set of values for the coupling constant gρσγby considering the ranges Mσ=400-1200MeV,Γσ=600-1000MeV for the parameters of theσ-meson.In terms of the invariant amplitude M(Eγ,E1),the differential decay probability for an unpolarizedρ0-meson at rest is given bydΓ(2π)31Γ= Eγ,max.Eγ,min.dEγ E1,max.E1,min.dE1dΓ[−2E2γMρ+3EγM2ρ−M3ρ2(2EγMρ−M2ρ)±Eγfunction ofβin Fig.5.This ratio is defined byΓβRβ=,Γtot.= Eγ,max.50dEγdΓdEγ≃constant.(10)ΓσM3σFurthermore,the values of the coupling constant gρσγresulting from our estimation are in general quite different than the values of this constant usually adopted for the one-meson exchange mechanism calculations existing in the literature.For example,Titov et al.[10] uses the value gρσγ=2.71which they obtain from Friman and Soyeur’s[9]analysis ofρ-meson photoproduction using Vector Meson Dominance.It is interesting to note that in their study of pion dynamics in Quantum Hadrodynamics II,which is a renormalizable model constructed using local gauge invariance based on SU(2)group,that has the sameLagrangian densities for the vertices we use,Serot and Walecka[14]come to the conclusion that in order to be consistent with the experimental result that s-waveπN-scattering length is anomalously small,in their tree-level calculation they have to choose gσππ=12.Since they use Mσ=520MeV this impliesΓσ≃1700MeV.If we use these values in our analysis,we then obtain gρσγ=11.91.Soyeur[12],on the other hand,uses quite arbitrarly the values Mσ=500 MeV,Γσ=250MeV,which in our calculation results in the coupling constant gρσγ=6.08.We like to note,however,that these values forσ-meson parameters are not consistent with the experimental data onσ-meson[11].Our analysis and estimation of the coupling constant gρσγusing the experimental value of the branching ratio of the radiative decayρ0→π++π−+γgive quite different values for this coupling constant than used in the literature.Furthermore,since we obtain this coupling constant as a function ofσ-meson parameters,it will be of interest to study the dependence of the observables of the reactions,such as for example the photoproduction of vector mesons on nucleonsγ+N→N+V where V is the neutral vector meson, analyzed using one-meson exchange mechanism on these parameters.AcknowledgmentsWe thank Prof.Dr.M.P.Rekalo for suggesting this problem to us and for his guidance during the course of our work.We also wish to thank Prof.Dr.T.M.Aliev for helpful discussions.REFERENCES[1]P.Singer,Phys.Rev.130(1963)2441;161(1967)1694.[2]V.N.Baier and V.A.Khoze,Sov.Phys.JETP21(1965)1145.[3]S.M.Renard,Nuovo Cim.62A(1969)475.[4]K.Huber and H.Neufeld,Phys.Lett.B357(1995)221.[5]E.Marko,S.Hirenzaki,E.Oset and H.Toki,Phys.Lett.B470(1999)20.[6]R.L.Jaffe,hep-ph/0001123.[7]M.Alford and R.L.Jaffe,hep-lat/0001023.[8]Aachen-Berlin-Bonn-Hamburg-Heidelberg-Munchen Collaboration,Phys.Rev.175(1968)1669.[9]B.Friman and M.Soyeur,Nucl.Phys.A600(1996)477.[10]A.I.Titov,T.-S.H.Lee,H.Toki and O.Streltrova,Phys.Rev.C60(1999)035205.[11]Review of Particle Physics,Eur.Phys.J.C3(1998)1.[12]M.Soyeur,nucl-th/0003047.[13]S.I.Dolinsky,et al,Phys.Rep.202(1991)99.[14]B.D.Serot and J.D.Walecka,in Advances in Nuclear Physics,edited by J.W.Negeleand E.Vogt,Vol.16(1986).TABLESTABLE I.The calculated coupling constant gρσγfor differentσ-meson parametersΓσ(MeV)gρσγ500 6.97-6.00±1.58 8008.45±1.77600 6.16-6.68±1.85 80010.49±2.07800 5.18-9.11±2.64 90015.29±2.84900 4.85-10.65±3.14 90017.78±3.23Figure Captions:Figure1:Diagrams for the decayρ0→π++π−+γFigure2:The photon spectra for the decay width ofρ0→π++π−+γ.The contributions of different terms are indicated.Figure3:The pion energy spectra for the decay width ofρ0→π++π−+γ.The contri-butions of different terms are indicated.Figure4:The decay width ofρ0→π++π−+γas a function of minimum detected photon energy.Figure5:The ratio Rβ=Γβ。
Dropping Sigma-Meson Mass and In-Medium S-Wave pion-pion Correlations
a r X i v :n u c l -t h /990876v 2 31 A u g 1999Dropping σ-Meson Mass and In-Medium S-wave π-πCorrelationsZ.Aouissat 1,G.Chanfray 2,P.Schuck 3,J.Wambach 11IKP,Technische Universit¨a t Darmstadt,Schloßgartenstraße 9,64289Darmstadt,Germany.2IPN Lyon.43Bd.du 11Novembre 1918,F69622Villeurbanne C´e dex,France.3ISN,Universite Joseph Fourier,CNRS-IN2P3,53avenue des Martyrs,F-38026Grenoble C´e dex,France.(February 9,2008)The influence of a dropping σ-meson mass on previously calculated in-medium ππcorrelations in the J =I =0(σ-meson)channel [2,?]is investigated.It is found that the invariant-mass distribution around the vacuum threshold experiences a further strong enhancement with respect to standard many-body effects.The relevance of this result for the explanation of recent A (π,2π)X data is pointed out.In medium s-wave pion-pion correlations have recently attracted much attention both on the theoretical [1–7]and experimental [8]sides.These studies are of rele-vance for the behavior of the in-medium chiral conden-sate and its fluctuations with increasing density [7].In earlier studies we have shown that standard p-wave cou-pling of the pion to ∆-h and p-h configurations induces a strong enhancement of the ππinvariant-mass distri-bution around the 2m πthreshold [2,3],thus signalling increased fluctuations in the σ-channel.This fact was in-dependently confirmed in [5].It has been argued in [2,4]that this effect could possibly explain the A (π,2π)knock-out reaction data from the CHAOS collaboration [8].More recently Vicente Vacas and Oset [6]have claimed that the theory underestimates the experimentally found π−πmass enhancement.This claim may be partly ques-tioned,since the reaction theory calls for a calculation with a finite total three momentum of the in-medium pion pairs ∗.On the other hand Hatsuda et al.[7]ar-gued that the partial restoration of chiral symmetry in nuclear matter,which leads to a dropping of the σ-meson mass [10],induces similar effects as the standard many-body correlation mentioned above.It is therefore natural to study the combination of both effects.This is the ob-jective of the present note.As a model for ππscattering we consider the lin-ear sigma model treated in leading order of the 1/N -expansion [9].The scattering matrix can then be cast in the following formT ab,cd (s )=δab δcdD −1π(s )−D −1σ(s )D π(s ),(1)where s is the Mandelstam variable.In Eq.(1)D π(s )s −m 2π,f π=√1−λ2Σππ(s )−1,(3)where Σππ(s )is the ππself-energy regularized by meansof a form factor which is used as a fit function [2]and allows to reproduce the experimental ππphase shifts.The coupling constant λ2denotes the bare quartic cou-pling of the linear σ-model,related to the mean-field pion mass m π,sigma mass m σ,and the condensate σ via the mean-field saturated Ward identitym 2σ=m 2π+2λ2 σ 2.(4)It is clear from what was said above that the σ-mesonpropagator in this approach is correctly defined,since it satisfies a whole hierarchy of Ward identities.In cold nuclear matter the pion is dominantly coupled to ∆-h,p-h,as well as to the 2p-2h excitations which,on the other hand,are renormalized by means of re-pulsive nuclear short-range correlations,(see [3]for de-tails).Since the pion is a (near)Goldstone mode,its in-medium s-wave renormalization does not induce con-siderable changes.The sigma meson,on the other hand,is not protected against an important s-wave renormal-ization from chiral symmetry.Therefore,following a very economical procedure,we extract an approximate density 1dependence of the mean-field sigma meson mass by tak-ing into account the density dependence of the conden-sate.From eq.(4)it is clear that the density dependence of the sigma-meson is essentially dictated by the density dependence of the condensate.FIG.1.Results for the imaginary part of the in-medium sigma-meson propagator.Except for the vacuum case(full line curve)the remaining in-medium curves are computed at normal nuclear matter density.The dashed-dotted curve is forα=0,dashed forα=0.2and the dotted forα=0.3. For densities below and around nuclear saturation den-sityρ0we take for the in-medium sigma-meson mass the simple ansatz(see also[7])ρmσ(ρ)=mσ(1−α[1]P.Schuck,W.N¨o renberg,G.Chanfray,Z.Phys.A330(1988)119.[2]P.Schuck,Z.Aouissat,F.Bonutti,G.Chanfray,E.Fra-giacomo,N.Grion,J.Wambach,Proceedings of the XXXVI,international Winter Meetingon Nuclear Physics,Ed.I.Iori,Bormio(Italy),January1998;nucl-th/9806069.[3]Z.Aouissat,R.Rapp,G.Chanfray,P.Schuck,J.Wambach,Nucl.Phys.A581(1995)471.[4]R.Rapp,J.W.Durso,Z.Aouissat,G.Chanfray,O.Krehl,P.Schuck,J.Speth,J.Wambach,Phys.Rev.C59(1999)R1237.[5]H.C.Chiang,E.Oset,M.J.Vicente Vacas,Nucl.Phys.A644(1998)77.[6]M.J.Vicente Vacas,E.Oset,nucl-th/9907008[7]T.Hatsuda,T.Kunihiro,H.Shimizu,Phys.Rev.Lett.82(1999)2840.[8]F.Bonutti et al.,Phys.Rev.Lett.77(1996)603.[9]Z.Aouissat,P.Schuck,J.Wambach,Nucl.Phys.A.618(1997)402.[10]G.E.Brown,M.Rho,Phys.Rep.269(1996)333.[11]D.Davesne,Y.J.Zhang,G.Chanfray,to be published.[12]Z.Aouissat,Ph.D.Thesis Report,ISN93-63,Grenoble.3。
sigmaaldrich引用 英文全名
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liquid–liquid phase separation
Biophysical Chemistry 109(2004)105–1120301-4622/04/$-see front matter ᮊ2003Elsevier B.V .All rights reserved.doi:10.1016/j.bpc.2003.10.021Cloud-point temperature and liquid–liquid phase separation ofsupersaturated lysozyme solutionJie Lu *,Keith Carpenter ,Rui-Jiang Li ,Xiu-Juan Wang ,Chi-Bun Ching a ,a a b bInstitute of Chemical and Engineering Sciences,Ayer Rajah Crescent 28,࠻02-08,Singapore 139959,Singapore aChemical and Process Engineering Center,National University of Singapore,Singapore 117576,SingaporebReceived 31July 2003;received in revised form 8October 2003;accepted 16October 2003AbstractThe detailed understanding of the structure of biological macromolecules reveals their functions,and is thus important in the design of new medicines and for engineering molecules with improved properties for industrial applications.Although techniques used for protein crystallization have been progressing greatly,protein crystallization may still be considered an art rather than a science,and successful crystallization remains largely empirical and operator-dependent.In this work,a microcalorimetric technique has been utilized to investigate liquid–liquid phase separation through measuring cloud-point temperature T for supersaturated lysozyme solution.The effects of cloud ionic strength and glycerol on the cloud-point temperature are studied in detail.Over the entire range of salt concentrations studied,the cloud-point temperature increases monotonically with the concentration of sodium chloride.When glycerol is added as additive,the solubility of lysozyme is increased,whereas the cloud-point temperature is decreased.ᮊ2003Elsevier B.V .All rights reserved.Keywords:Biocrystallization;Microcalorimetry;Cloud-point temperature;Liquid–liquid phase separation1.IntroductionKnowledge of detailed protein structure is essen-tial for protein engineering and the design of pharmaceuticals.Production of high-quality pro-tein crystals is required for molecular structure determination by X-ray crystallography.Although considerable effort has been made in recent years,obtaining such crystals is still difficult in general,and predicting the solution conditions where pro-*Corresponding author.Tel.:q 65-6874-4218;fax:q 65-6873-4805.E-mail address:lujie@.sg (J.Lu ).teins successfully crystallize remains a significant obstacle in the advancement of structural molecu-lar biology w 1x .The parameters affecting protein crystallization are typically reagent concentration,pH,tempera-ture,additive,etc.A phase diagram can provide the method for quantifying the influence of solu-tion parameters on the production of crystals w 2,3x .To characterize protein crystallization,it is neces-sary to first obtain detailed information on protein solution phase behavior and phase diagram.Recently physics shows that there is a direct relationship between colloidal interaction energy106J.Lu et al./Biophysical Chemistry109(2004)105–112and phase diagram.Gast and Lekkerkerker w4,5x have indicated that the range of attraction between colloid particles has a significant effect on the qualitative features of phase diagram.A similar relationship should hold for biomacromolecules, i.e.the corresponding interaction potentials govern the macromolecular distribution in solution,the shape of the phase diagram and the crystallization process w6x.Many macromolecular crystallizations appear to be driven by the strength of the attractive interactions,and occur in,or close to,attractive regimes w7,8x.Recent intensive investigation has revealed that protein or colloidal solution possesses a peculiar phase diagram,i.e.liquid–liquid phase separation and sol–gel transition exists in general in addition to crystallization w9,10x.The potential responsible for the liquid–liquid phase separation is a rather short range,possibly van der Waals,attractive potential w11,12x.The measurement of cloud-point temperature T can provide useful informationcloudon the net attractive interaction between protein molecules,namely,the higher the cloud-point tem-perature,the greater the net attractive interaction. Herein Taratuta et al.w13x studied the effects of salts and pH on the cloud-point temperature of lysozyme.Broide et al.w14x subsequently meas-ured the cloud-point temperature and crystalliza-tion temperature for lysozyme as a function of salt type and concentration.From these works the cloud-point temperature was found to be typically 15–458C below the crystallization temperature. Furthermore,Muschol and Rosenberger w15x deter-mined the metastable coexistence curves for lyso-zyme through cloud-point measurements,and suggested a systematic approach to promote pro-tein crystallization.In general,an effective way to determine the strength of protein interactions is to study temperature-induced phase transitions that occur in concentrated protein solutions.Liquid–liquid phase separation can be divided into two stages w11x:(1)the local separation stage at which the separation proceeds in small regions and local equilibrium is achieved rapidly;and(2) the coarsening stage at which condensation of these small domains proceeds slowly to reduce the loss of interface free energy w16x.The coexisting liquid phases both remain supersaturated but differ widely in protein concentration.The effect of a metastable liquid–liquid phase separation on crystallization remains ambiguous w17x.Molecular dynamics simulations and analyt-ical theory predict that the phase separation will affect the kinetics and the mechanisms of protein crystal nucleation w18x.tenWolde and Frenkel w19x have demonstrated that the free energy barrier for crystal nucleation is remarkably reduced at the critical point of liquid–liquid phase separation, thus in general,after liquid–liquid phase separa-tion,crystallization occurs much more rapidly than in the initial solution,which is typically too rapid for the growth of single crystal with low defect densities w15x.The determination of the location of liquid–liquid phase separation curve is thus crucial for efficiently identifying the optimum solution conditions for growing protein crystals. Microcalorimetry has the potential to be a useful tool for determining:(1)the metastable-labile zone boundary;(2)the temperature-dependence of pro-tein solubility in a given solvent;and(3)the crystal-growth rates as a function of supersatura-tion w20x.Microcalorimeters can detect a power signal as low as a few microwatts whereas standard calorimeters detect signals in the milliwatt range. Because of this greater sensitivity,samples with small heat effects can be analyzed.In addition, microcalorimetry has the advantage of being fast, non-destructive to the protein and requiring a relatively small amount of material.The present work is concerned with the analysis of the transient heat signal from microcalorimeter to yield liquid–liquid phase separation information for lysozyme solutions at pH4.8.To further examine the role of salt and additive on interprotein interactions, cloud-point temperature T has been determinedcloudexperimentally as a function of the concentrations of salt,protein and glycerol.2.Materials and methods2.1.MaterialsSix times crystallized lysozyme was purchased from Seikagaku Kogyo,and used without further107J.Lu et al./Biophysical Chemistry 109(2004)105–112purification.All other chemicals used were of reagent grade,from Sigma Chemical Co.2.2.Preparation of solutionsSodium acetate buffer (0.1M )at pH 4.8was prepared with ultrafiltered,deionized water.Sodi-um azide,at a concentration of 0.05%(w y v ),was added to the buffer solution as an antimicrobial agent.Protein stock solution was prepared by dissolving protein powder into buffer.To remove undissolved particles,the solution was centrifuged in a Sigma centrifuge at 12000rev.y min for 5–10min,then filtered through 0.22-m m filters (Mil-lex-VV )into a clean sample vial and stored at 48C for further experiments.The concentration of protein solution was determined by measuring the absorbance at 280nm of UV spectroscopy (Shi-madzu UV-2550),with an extinction coefficient of 2.64ml y (mg cm )w 21x .Precipitant stock solution was prepared by dissolving the required amount of sodium chloride together with additive glycerol into buffer.The pH of solutions was measured by a digital pH meter (Mettler Toledo 320)and adjusted by the addition of small volumes of NaOH or HAc solution.2.3.Measurement of solubilitySolubility of lysozyme at various temperatures and precipitant y additive concentrations was meas-ured at pH 4.8in 0.1M acetate buffer.Solid–liquid equilibrium was approached through both crystallization and dissolution.Dissolving lasted 3days,while the period of crystallization was over 2weeks.The supernatant in equilibrium with a macroscopically observable solid was then filtered through 0.1-m m filters (Millex-VV ).The concen-tration of diluted supernatant was determined spec-troscopically and verified by refractive meter(Kruss)until refractive index remained unchanged ¨at equilibrium state.Solubility of each sample was measured in duplicate.2.4.Differential scanning microcalorimetry Calorimetric experiments were performed with a micro-differential scanning calorimeter with anultra sensitivity,micro-DSC III,from Setaram SA,France.The micro-DSC recorded heat flow in microwatts vs.temperature,thus can detect the heat associated with phase transition during a temperature scan.The sample made up of equal volumes of protein solution and precipitant solu-tion was filtered through 0.1-m m filters to remove dust particles further.To remove the dissolved air,the sample was placed under vacuum for 3min while stirring at 500rev.y min by a magnetic stirrer.The degassed sample was placed into the sample cell of 1.0ml,and a same concentration NaCl solution was placed into the reference cell.The solutions in the micro-DSC were then cooled at the rate of 0.28C y min.After every run,the cells were cleaned by sonicating for 10–15min in several solutions in the following order:deionized water,methanol,ethanol,acetone,1M KOH and finally copious amounts of deionized water.This protocol ensured that lysozyme was completely removed from the cells.The cells were then placed in a drying oven for several hours.The rubber gaskets were cleaned in a similar manner except acetone and 1M KOH were omitted and they were allowed to dry at low temperature.3.Results and discussionA typical micro-DSC scanning experiment is shown in Fig.1.The onset of the clouding phe-nomenon is very dramatic and easily detected.The sharp increase in the heat flow is indicative of a liquid–liquid phase separation process producing a latent heat.This is much consistent with many recent investigations of the liquid–liquid phase separation of lysozyme from solution w 22,23x .In fact,such a liquid–liquid phase separation is a phase transition with an associated latent heat of demixing.In this work,the cloud-point tempera-tures at a variety of lysozyme,NaCl and glycerol concentrations are determined by the micro-DSC at the scan rate of 128C y h.3.1.Effect of protein concentrationIn semilogarithmic Fig.2we plot the solid–liquid and liquid–liquid phase boundaries for lyso-108J.Lu et al./Biophysical Chemistry 109(2004)105–112Fig.1.Heat flow of a typical micro-DSC scan of lysozyme solution,50mg y ml,0.1M acetate buffer,pH 4.8,3%NaCl.The scan rate 128C y h is chosen referenced to the experimental results of Darcy and Wiencek w 23x .Note the large deflection in the curve at approximately 4.38C indicating a latent heat resulting from demixing (i.e.liquid–liquid phase separation )process.Fig.2.Cloud-point temperature and solubility determination for lysozyme in 0.1M acetate buffer,pH 4.8:solubility (5%NaCl )(s );T (5%NaCl,this work )(d );T (5%cloud cloud NaCl,the work of Darcy and Wiencek w 23x )(*);solubility (3%NaCl )(h );T (3%NaCl )(j ).cloud Fig.3.Cloud-point temperature determination for lysozyme as a function of the concentration of sodium chloride,50mg y ml,0.1M acetate buffer,pH 4.8.zyme in 0.1M acetate buffer,pH 4.8,for a range of protein concentrations.It is worth noting that,at 5%NaCl,our experimental data of T from cloud micro-DSC are quite consistent with those from laser light scattering and DSC by Darcy and Wiencek w 23x ,with difference averaging at approx-imately 0.88C.This figure demonstrates that liquid–liquid phase boundary is far below solid–liquid phase boundary,which implies that the liquid–liquid phase separation normally takes place in a highly metastable solution.In addition,cloud-point temperature T increases with the cloud concentration of protein.3.2.Effect of salt concentrationFig.3shows how cloud-point temperature changes as the concentration of NaCl is varied from 2.5to 7%(w y v ).The buffer is 0.1M acetate (pH 4.8);the protein concentration is fixed at 50mg y ml.Over the entire range of salt concentrations studied,the cloud-point temperature strongly depends on the ionic strength and increases monotonically with the concentration of NaCl.Crystallization is driven by the difference in chemical potential of the solute in solution and in the crystal.The driving force can be simplified as w 24xf sy Dm s kT ln C y C (1)Ž.eq109J.Lu et al./Biophysical Chemistry 109(2004)105–112Fig.4.The driving force required by liquid–liquid phase sep-aration as a function of the concentration of sodium chloride,50mg y ml lysozyme solution,0.1M acetate buffer,pH 4.8.In the same way,we plot the driving force,f ,required by liquid–liquid phase separation as a function of the concentration of sodium chloride in Fig.4.At the moderate concentration of sodium chloride,the driving force required by liquid–liquid phase separation is higher than that at low or high salt concentration.As shown in Fig.3,with NaCl concentration increasing,the cloud-point temperature increases,which is in accord with the results of Broide et al.w 14x and Grigsby et al.w 25x .It is known that protein interaction is the sum of different potentials like electrostatic,van der Waals,hydrophobic,hydration,etc.The liquid–liquid phase separation is driven by a net attraction between protein molecules,and the stronger the attraction,the higher the cloud-point temperature.Ionic strength is found to have an effect on the intermolecular forces:attractions increase with ionic strength,solubility decreases with ionic strength,resulting in the cloud-point temperature increases with ionic strength.It is worth noting that,the effect of ionic strength on cloud-point temperature depends strongly on the specific nature of the ions w 13x .Kosmotropic ions bind adjacent water molecules more strongly than water binds itself.When akosmotropic ion is introduced into water,the entro-py of the system decreases due to increased water structuring around the ion.In contrast,chaotropes bind adjacent water molecules less strongly than water binds itself.When a chaotrope is introduced into water,the entropy of the system increases because the water structuring around the ion is less than that in salt-free water.This classification is related to the size and charge of the ion.At high salt concentration ()0.3M ),the specific nature of the ions is much more important w 25x .The charges on a protein are due to discrete positively and negatively charged surface groups.In lysozyme,the average distance between thesecharges is approximately 10Aw 26x .As to the salt ˚NaCl used as precipitant,Na is weakly kosmo-q tropic and Cl is weakly chaotropic w 27x .At low y NaCl concentrations,as the concentration of NaCl increases,the repulsive electrostatic charge–charge interactions between protein molecules decrease because of screening,resulting in the increase of cloud-point temperature.While at high NaCl con-centrations,protein molecules experience an attrac-tion,in which differences can be attributed to repulsive hydration forces w 14,25x .That is,as the ionic strength increases,repulsive electrostatic or hydration forces decrease,protein molecules appear more and more attractive,leading to higher cloud-point temperature.At various salt concentra-tions,the predominant potentials reflecting the driving force for liquid–liquid phase separation are different.Fig.4shows that the driving force,f ,is parabolic with ionic strength,while Grigsby et al.w 25x have reported that f y kT is linear with ionic strength for monovalent salts.The possible reasons for that difference include,their model is based on a fixed protein concentration of 87mg y ml,which is higher than that used in our study,yet f y kT is probably dependent on protein concentration,besides the solutions at high protein and salt concentrations are far from ideal solutions.3.3.Effect of glycerolFig.5compares cloud-point temperature data for 50mg y ml lysozyme solutions in absence of glycerol and in presence of 5%glycerol,respec-110J.Lu et al./Biophysical Chemistry109(2004)105–112parison of cloud-point temperatures for lysozyme at different glycerol concentrations as a function of the con-centration of sodium chloride,50mg y ml,0.1M acetate buffer, pH4.8:0%glycerol(s);5%glycerol(j).Fig.6.Cloud-point temperatures for lysozyme at different glycerol concentrations,50mg y ml lysozyme,5%NaCl,0.1M acetate buffer,pH4.8.Fig.7.Cloud-point temperature and solubility determination for lysozyme at different concentrations of glycerol in0.1M acetate buffer,5%NaCl,pH4.8:solubility(0%glycerol)(s); T(0%glycerol)(d);solubility(5%glycerol)(h);cloudT(5%glycerol)(j).cloudtively.Fig.6shows the cloud-point temperature as a function of the concentration of glycerol.The cloud-point temperature is decreased as the addi-tion of glycerol.In semilogarithmic Fig.7we plot the solid–liquid and liquid–liquid phase boundaries at dif-ferent glycerol concentrations for lysozyme in0.1 M acetate buffer,5%NaCl,pH4.8,for a range of protein concentration.This figure demonstrates that liquid–liquid and solid–liquid phase bounda-ries in the presence of glycerol are below those in absence of glycerol,and the region for growing crystals is narrowed when glycerol is added. Glycerol has the property of stabilizing protein structure.As a result,if crystallization occurs over a long period of time,glycerol is a useful candidate to be part of the crystallization solvent and is often included for this purpose w28x.In addition,glycerol is found to have an effect on the intermolecular forces:repulsions increase with glycerol concentra-tion w29x.Our experiment results of solubility and cloud-point temperature can also confirm the finding.The increased repulsions induced by glycerol can be explained by a number of possible mecha-nisms,all of which require small changes in the protein or the solvent in its immediate vicinity.The addition of glycerol decreases the volume of protein core w30x,increases hydration and the size of hydration layer at the particle surface w31,32x. In this work,we confirm that glycerol shifts the solid–liquid and liquid–liquid phase boundaries. The effect of glycerol on the phase diagram strong-111 J.Lu et al./Biophysical Chemistry109(2004)105–112ly depends on its concentration and this canprovide opportunities for further tuning of nuclea-tion rates.4.ConclusionsGrowing evidence suggests protein crystalliza-tion can be understood in terms of an order ydisorder phase transition between weakly attractiveparticles.Control of these attractions is thus keyto growing crystals.The study of phase transitionsin concentrated protein solutions provides one witha simple means of assessing the effect of solutionconditions on the strength of protein interactions.The cloud-point temperature and solubility datapresented in this paper demonstrate that salt andglycerol have remarkable effects on phase transi-tions.The solid–liquid and liquid–liquid bounda-ries can be shifted to higher or lower temperaturesby varying ionic strength or adding additives.Ourinvestigation provides further information upon therole of glycerol used in protein crystallization.Glycerol can increase the solubility,and decreasethe cloud-point temperature,which is of benefit totuning nucleation and crystal growth.In continuingstudies,we will explore the effects of other kindsof additives like nonionic polymers on phasetransitions and nucleation rates.Much more theo-retical work will be done to fully interpret ourexperimental results.AcknowledgmentsThis work is supported by the grant from theNational Natural Science Foundation of China(No.20106010).The authors also thank Professor J.M.Wiencek(The University of Iowa)for kinddiscussion with us about the thermal phenomenaof liquid–liquid phase separation.Referencesw1x A.McPherson,Current approaches to macromolecular crystallization,Eur.J.Biochem.189(1990)1–23.w2x A.M.Kulkarni, C.F.Zukoski,Nanoparticle crystal nucleation:influence of solution conditions,Langmuir18(2002)3090–3099.w3x E.E.G.Saridakis,P.D.S.Stewart,L.F.Lloyd,et al., Phase diagram and dilution experiments in the crystal-lization of carboxypeptidase G2,Acta Cryst.D50(1994)293–297.w4x A.P.Gast, C.K.Hall,W.B.Russel,Polymer-induced phase separations in non-aqueous colloidal suspensions,J.Colloid Interf.Sci.96(1983)251–267.w5x H.N.W.Lekkerkerker,W.C.K.Poon,P.N.Pusey,et al., Phase-behavior of colloid plus polymer mixtures,Euro-phys.Lett.20(1992)559–564.w6x A.Tardieu,S.Finet,F.Bonnete,Structure of the´macromolecular solutions that generate crystals,J.Cryst.Growth232(2001)1–9.w7x D.Rosenbaum,C.F.Zukoski,Protein interactions and crystallization,J.Cryst.Growth169(1996)752–758.w8x A.George,W.W.Wilson,Predicting protein crystalli-zation from a dilute solution property,Acta Cryst.D50(1994)361–365.w9x D.Rosenbaum,P.C.Zamora, C.F.Zukoski,Phase-behavior of small attractive colloidal particles,Phys.Rev.Lett.76(1996)150–153.w10x V.J.Anderson,H.N.W.Lekkerkerker,Insights into phase transition kinetics from colloid science,Nature416(2002)811–815.w11x S.Tanaka,K.Ito,R.Hayakawa,Size and number density of precrystalline aggregates in lysozyme crys-tallization process,J.Chem.Phys.111(1999)10330–10337.w12x D.W.Liu,A.Lomakin,G.M.Thurston,et al.,Phase-separation in multicomponent aqueous-protein solutions,J.Phys.Chem.99(1995)454–461.w13x V.G.Taratuta,A.Holschbach,G.M.Thurston,et al., Liquid–liquid phase separation of aqueous lysozymesolutions:effects of pH and salt identity,J.Phys.Chem.94(1990)2140–2144.w14x M.L.Broide,T.M.Tominc,M.D.Saxowsky,Using phase transitions to investigate the effect of salts onprotein interactions,Phys.Rev.E53(1996)6325–6335. w15x M.Muschol,F.Rosenberger,Liquid–liquid phase sep-aration in supersaturated lysozyme solutions and asso-ciated precipitate formation y crystallization,J.Chem.Phys.107(1997)1953–1962.w16x C.Domb,J.H.Lebowitz,Phase Separation and Critical Phenomena,Academic,London,1983.w17x D.F.Rosenbaum,A.Kulkarni,S.Ramakrishnan,C.F.Zukoski,Protein interactions and phase behavior:sen-sitivity to the form of the pair potential,J.Chem.Phys.111(1999)9882–9890.w18x 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crystal growth,J.Cryst.Growth209(2000)130–137.w25x J.J.Grigsby,H.W.Blanch,J.M.Prausnitz,Cloud-point temperatures for lysozyme in electrolyte solutions:effectof salt type,salt concentration and pH,Biophys.Chem.91(2001)231–243.w26x D.Voet,J.Voet,Biochemistry,Wiley,New Y ork,1990. w27x K.D.Collins,Charge density-dependent strength of hydration and biological structure,Biophys.J.72(1997)65–76.w28x R.Sousa,Use of glycerol and other protein structure stabilizing agents in protein crystallization,Acta Cryst.D51(1995)271–277.w29x M.Farnum, C.F.Zukoski,Effect of glycerol on the interactions and solubility of bovine pancreatic trypsininhibitor,Biophys.J.76(1999)2716–2726.w30x A.Priev,A.Almagor,S.Yedgar,B.Gavish,Glycerol decreases the volume and compressibility of proteininterior,Biochemistry35(1996)2061–2066.w31x S.N.Timasheff,T.Arakawa,Mechanism of protein precipitation and stabilization by co-solvents,J.Cryst.Growth90(1988)39–46.w32x C.S.Miner,N.N.Dalton,Glycerol,Reinhold Publishing, New Y 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阿尔法拉瓦尔(Alfa Laval)双相作用Sigma系列分心机用于橄榄油提取的应用说明说明书
ApplicationAlfa Laval introduces the Sigma series of decanter cen-trifuges specially designed for olive oil extraction intwo-phase operation. The main focus in this successful development has been to maximize oil recovery through-out the fl ow range, while maintaining the highest oil quality by m inimizing any temperature increase in the separation process.Decanter centrifuges in the Alfa Laval Sigma series are designed to ensure cost-effective operation in the oliveoil processing chain, with a specifi c focus on two-phase operation. These compact, effi cient decanters are opti-mized for olive oil applications that include clarifi cation, extraction, dewatering and classifi cation on the fi rst and second extraction (re-milling).Sigma decanters performances can be enhanced withAL Thermal Conditioning Module, a high-tech system for controlling temperature in olive oil extraction.Design features and benefi tsSigma decanter centrifuges feature a special designo ptimized for two-phase operation. Particular features include:• F ully protected feed zone, benefi ting from a new design • S pecial conveyor design to improve solids transportation and maximize oil recovery• S pecial fl ight for oil migration to the liquid outlet, designed to help optimize yield and oil clarifi cation by avoiding turbulence• 360° solids discharge outlet made of tungsten carbide, for exceptional protection against wear• T ungsten carbide tiles on conveyor, for special wear protection that signifi cantly reduces maintenance costs • C ompact design with small footprintElectronic control of the conveyor speed (differential speed) means the retention time can be adjusted and set to obtain maximum oil recovery. Electronic control of differential speed is carried out via a variable frequency drive (VFD) directly connected to the exclusive Alfa Laval direct drive gearbox. This setup ensures minimum power consumption as well as reduced heating of the oil.Alfa Laval Sigma decanter centrifuges also feature an electronic overload protection system.High-quality stainless steel is used throughout. The casing is hinged for easy opening, maintenance and cleaning.The Sigma 9 decanter centrifuge – fi rst in the new Alfa Laval Reverse Decanter Sigma RangeA l f a L a v a l i s a t r a d e m a r k r e g i s t e r e d a n d o w n e d b y A l f a L a v a l C o r p o r a t e AB .P F L 00065E N 1512Alfa Laval reserves the right to change specifications without prior notification.How to contact Alfa LavalUp-to-date Alfa Laval contact details for all countries are always available on our website at Standard equipmentAlfa Laval Sigma decanter centrifuges include the following as standard equipment:• S olids chute and oil pipe • T ank equipped with filter suitable for collection of the oil • S et of spare parts • S et of tools for disassembly and maintenance • L ubricant oil and grease with the appropriate guns Control panel with back-drive system for electronically adjust-ing the conveyor screw differential speed and measuring conveyor torque.Operating principleThe Sigma decanter centrifuge design ensures separation of the incoming olive paste into two phases – oil and wet solids. The olive paste is fed into the bowl through a station-ary inlet tube and is then smoothly accelerated by an inlet rotor. Separation takes place in a horizontal cylindrical bowl equipped with a screw conveyor. Centrifugal force causes the oil to accumulate at the liquid surface in the decanter, while the solids settle on the inner wall of the bowl surrounded by the water separated out of the feed stream.The conveyor rotates at a slightly different speed than the bowl, and conveys the solids to the discharge in the conical end. Separation takes place along the entire length of the cylindrical part of the bowl. The oil is discharged through the large end of the bowl and passes into collecting tanks via a filter.Technical dataBowl diameter diameter 550 mm / 86.61 inches Bowl length2200 mm / 86.61 inchesBowl speed (maximum) 3400 rpm G-force (maxmum) 3554 GWeight 5000 kg / 11023 lbsInstalled power 77 kW (103.3 hp)Maximum length 5722 mm / 225.27 inches Width 1300 mm / 51.18 inches Height1693 mm / 77.28 inchesTwo-phase operationDimensions GearboxScrew conveyorWall of the bowlConical endDischarge portFeed tubeSolids with waterLight liquid phase。
淋浴房配件中英文对照
2 品名Name of an article3 房体Room body4 顶盖Top cover5 不含功能板背板Do not include the function board backplate6 置物架Supporter7 小号毛巾架脚套Foot set of small size towel rail8 大扁自攻螺丝Big flat self-tapping screw9 毛巾架Towel rail10 弯扶手Curved handrail11 小孔装饰脚The aperture decorates the foot12 不锈钢外六角螺丝Six angle screws outside stainless steel13 银镜Silver mirror14 银镜螺丝、螺母Silver mirror screw , nut15 按摩器挂扣The massager is hung and deducted16 升降架Hoist or lower the shelf17 连接座、支撑座Join seats , support seats18 大孔装饰脚The big hole decorates the foot19 房体铝合金Room body aluminium alloy20 大扁自攻Attack by oneself big and flat21 太阳花洒The sun flower is shed22 铜螺母Copper nut23 灯盘Light plate24 防碰胶垫Defend and touch the glue cushion25 铁夹片The iron inserting slice26 内六角螺丝Six angle screws inside27 座位出水胶圈The seat surfaces the glue is enclosed28 平垫Flat cushion29 顶盖铝合金Top cover aluminium alloy30 产品说明书Catalogue31 售后服务卡After-sale service card32 密封袋Seal bag33 标签纸Label paper34 透明软管夹The transparent hose inserting35 夹片Insert slice36 螺丝Screw37 螺母Nut38 放水龙头The faucet put39 放水龙头锁紧螺母The faucet locknut put40 面板Panel41 小胶垫Small glue cushion42 大胶垫Big glue cushion43 空气调节Regulation of the air44 空气调节器Air conditioner45 空气调节拧盖The regulation of the air is twisted and covered46 空气调节面板Panel of regulation of the air47 空气调节拧盖螺母The regulation of the air is twisted and covered on the nut48 空气调节保护盖Visor of regulation of the air49 胶垫Glue cushion50 毛巾杆Towel pole51 毛巾支架Towel support52 毛巾架套Towel rail set53 介子Meson54 去水管Go the water pipe55 去水软管Go ink hose56 去水软管螺母Go ink hose nut57 透明管螺母Manage the nut transparently58 去水接头Water is connected to go59 大喷头Big shower nozzle60 大喷头座Big shower nozzle seat61 大喷头管头The big shower nozzle is managed one62 大喷头盖The big shower nozzle covering63 阀芯Core of the valve64 卡簧Card reed65 阀芯胶圈The valve core glue enclosing66 阀芯螺母Valve core nut67 阀芯座Valve core seat68 阀芯座胶圈The valve core seat glue enclosing69 标签Label70 地线标签Ground wire label71 花洒接驳螺丝The flower sheds and plugs into the screw72 合格证Certificate of quality73 灯泡(附送)Bulb ( Give as an addition)74 花洒清洗器The flower sheds the washing device75 冷热水胶垫(附送)Cold hot water glue cushion ( Give as an addition)76 发光二极条Secondary one of luminescence77 铁架封头The iron stand seals the head78 五通堵头Stop up the head five times79 带咀一寸半弯头Lead and chew one and a half inches of elbows80 置物杆Put the thing pole81 置物脚Put the foot of things82 连接铁Join irons83 置物架盖The supporter covering84 置物杆盖Put the thing pole to cover85 置物架介子Meson of the supporter86 去水器Water going device87 去水支架Go ink support88 去水拧盖Water is twisted and covered to go89 去水拧盖保护盖Water is twisted and covered on the visor to go90 去水拧盖螺丝Water is twisted and covered on the screw to go91 去水盖Water is covered to go92 去水防水胶垫Go water waterproof glue cushion93 去水器螺母Going to water device nut94 去水器压盖The water device is pressed and covered to go95 电脑Computer96 压边条Press the strake97 挡水玻璃Block the water glass98 防水胶条Waterproof adhesive tape99 压边条螺丝Press the strake screw100 水位探针Water level probe101 臭氧Ozone102 臭氧螺丝Ozone screw103 电脑螺丝Computer screw104 1.22米编织管1.22 meters weaving and managing105 喇叭Loudspeaker106 喇叭盖The loudspeaker covering107 固定螺丝Fixed screw108 商标Trade mark109 座缸Seat jar110 螺杆Spiral shell's pole111 冲压件Press one112 纤维毡Fibre felt113 浴缸上脚Foot on the bathtub114 浴缸下脚The bathtub gets a foothold115 四方封头盖Seal the skull all sides116 裙板螺丝、螺母Board screw of the skirt , nut117 防碰胶垫Defend and touch the glue cushion118 圆头自攻螺丝Self-tapping screw of button head119 铁架螺母Iron stand nut120 垫片Spacer121 脚踏去水器Ride the water going device122 去水器螺母Going to water device nut123 乌克兰大商标Ukraine's big trade mark124 铁夹片The iron inserting slice125 玻璃夹片The glass inserting slice126 新款喷头Shower nozzle of new style127 管夹The tube inserting128 花洒接头带底座The flower sheds and connects and takes the base129 活动弯头Activity elbow130 座位去水器橡胶圈The seat going to water device rubber enclosing131 单头管The single head managing132 无头管There is no head to manage133 铝合金Aluminium alloy134 电机Electrical machinery135 电机接口内管Manage in the interface of the electrical machinery136 电机接口外管Manage outside the interface of the electrical machinery137 防水垫Waterproof cushion138 电机三通Three direct links of the electrical machinery139 地线Ground wire140 电机螺杆Spiral shell's pole of the electrical machinery141 电机螺母Electrical machinery nut142 电机螺丝Electrical machinery screw143 地线螺丝Ground wire screw144 枕头Pillow145 支撑座Support seats146 镀铬塑料枕头套Plate the plastic pillowcase of chromium147 直径的喷头Shower nozzle of the diameter148 喷头螺母Shower nozzle nut149 喷头面盖Cover the shower nozzle150 喷头芯螺母Core nut of shower nozzle151 喷头座Shower nozzle seat152 喷头芯Core of the shower nozzle153 回水器Return water device154 回水弯头Return water elbow155 回水螺母Return water nut156 螺丝盖The screw covering157 回水盖螺母The return water is covered on the nut158 回水盖The return water covering159 功能龙头Function tap160 冷热水开关Cold hot water switch161 冷热水开关螺丝Cold hot water switch screw162 功能转换阀胶垫The function changes the valve glue cushion163 功能转换阀The function changes the valve164 冷热水开关保护盖Cold hot water switch visor165 功能转换阀保护盖The function changes the valve visor166 冷热水管Cold and hot water pipe167 冷热水开关面板Cold hot water switch panel168 功能转换阀面板The function changes the valve panel169 花洒插座The flower sheds the socket170 花洒管The flower shedding and managing171 花洒The flower shedding172 透明接头管(双头)Connect and manage transparently ( One pair of heads)173 透明接头管(单头)Connect and manage transparently ( Single head)174 池底灯Light of bottom of the pool176 池底灯注塑电镀面盖The light of bottom of the pool moulds plastics and electroplates the surface to cover177 池底灯透明灯盖The transparent light of the light of bottom of the pool is covered178 池底灯硅胶内防水垫Waterproof cushion in the light silica gel of bottom of the pool179 池底灯硅胶外防水垫Waterproof cushion outside the light silica gel of bottom of the pool180 池底灯泡Bulb of bottom of the pool181 池底灯泡座Bulb seat of bottom of the pool182 支架Support183 1.5寸软管1.5 inches of hoses184 6分软管6 points of hoses185 透明软管Transparent hose186 1.5寸弯头Elbow of 1.5 inches187 6分三通6 points of three direct links188 1寸三通An inch of three direct links189 空气五通Air 5 times190 1寸塞头An inch of gag191 6分塞头6 points of gags192 警告标识Warn identifications193 专利标识Patent identification194 扎带Pitch bringing195 底缸Bottom jar196 长裙板Long skirt board197 注塑夹Mould plastics to insert198 裙板上装饰螺帽Decorate the nut on the skirt board199 螺丝座Screw seat200 弹簧片Spring slice201 弹簧片不锈钢螺丝Spring film stainless steel screw202 小灯盖(半透明)The small light covering ( Semitransparent) 203 铁架Iron stand204 缸脚Foot of the jar205 缸脚螺母Jar foot nut206 螺杆介子Pole meson of spiral shell。
TW 142数学名词-中小学教科书名词中英对照术语
001 (ISSCC tutorial)Noise Analysis in Switched-Capacitor Circuits
© 2011 IEEE
IEEE International Solid-State Circuits Conference
© 2011 IEEE
Thermal Noise Power
• Nyquist showed that
PSD ( f ) = 4kT
• The total average noise power of a resistor in a certain frequency band is therefore
– Examples: Audio systems, wireless transceivers, sensor interfaces
• Electronic noise directly trades with power dissipation and speed • Electronic noise is a major concern in modern technologies with reduced VDD
• The noise of a MOSFET operating in the triode region is approximately equal to that of a resistor • In the saturation region, the thermal noise can be modeled using a drain current source with power spectral density
• We can model the noise using an equivalent voltage or current generator
2 vn
= Pn ⋅ R = 4kT ⋅ R ⋅ Δf
【doc】高能所2002年发表的专著及在学术刊物上公开发表的论文
高能所2002年发表的专著及在学术刊物上公开发表的论文附录3.发表论文目录141附录3.高能所2002年发表的专着及在学术刊物上公开发表的论文作者(以原序发表干U物名称序号专着或论文题目单位排列)卷号(年)页lFirstMeasurementofthe实验物理J.Z.Baieta1.Phys.Rev. BranchingFractionoftheDecay中心BESCollab.D65(2002)052004v/(2s,Z-+一2MeasurementoftheCrOSSSection实验物理J.Z.Baieta1.Phys.Rev.Lett.f0ree一hadronsat中心BESCollab.88(2002)101802Center-of-massEnergiesfrom2to5GeV3AMeasurementof(2)实验物理J.Z.Baicta1.Phys.Lett.中心BESCollab.B550(2002)24?32ResonanceParamcters4/.3(z7/"一)分支比实验物理BES合作组高能物理与核物理中心26(2002)8?16的测定5Decaysofthe/to∑..实验物理BES合作组高能物理与核物理FinalState中心26(2002)93?996中性和带电D介子单举半轻子实验物理BES合作组高能物理与核物理(电子)衰变分支比的测量中心26(2002)547?5567D和D.介子的遍举实验物理BES合作组高能物理与核物理分支比上限的测定中心26(2002)1093?11028北京谱仪II中性径迹测量误差的实验物理王君等高能物理与核物理确定中心26(2002)116-1219用联合D.和D单双标记测定实验物理荣刚等高能物理与核物理分支比的方法中心26(2002)207-215l0TEX0N0低能中微子实验中的实验物理李金等高能物理与核物理CsI(T1)晶体探测器中心26(2002)393-401l1TELESIS在3y衰变末态实验物理许国发等高能物理与核物理分析中的应用中心26(2002)462?470l2TEX0N0中微子实验屏蔽效果实验物理陈栋梁等高能物理与核物理的MonteCarlo研究中心26(2002)626?631l3在BEPCII/BESIII上寻找r/和实验物理李刚等高能物理与核物理中心26(2002)645-651态的MonteCar1o研究l4EnergyCalibrationofCsI(TI)实验物理岳骞等高能物理与核物理CrystalforQuenchingFactor中心26(2002)728?734 MeasurementinDarkMatterSearchl5最优化的北京谱仪取数时间实验物理苑长征等高能物理与核物理中心26(2002)759?765l42中国科学院高能物理研究所2002年《年报》豁幸●}案}謦謦沓尊l_专蓝l霉1'奄譬,鼍善童警{孳鼍上{点《l,作者(以原序发表刊物名称序号专着或论文题目单位排列)卷号(年)页l6MeasurementofQuenchingFactor实验物理岳骞等高能物理与核物理forNuclearRecoilsinCsI(TI)中心26(2002)855—860Crystall7北京谱仪(BESII)的飞行时间计实验物理彭海平等高能物理与核物理数器(1_0F)蒙特卡罗模拟的改进中心26(2002)86l一869l8TEXONO反应堆中微子能谱的实验物理陈栋梁,李金等高能物理与核物理计算中心26(2002)889—894l9R值测量中束流相关本底的扣除实验物理鄢文标等高能物理与核物理方法中心26(2002)998—100320北京谱仪(BESII)的飞行时间计实验物理彭海平等高能物理与核物理数器(TOF)时间和分辨律的修中心26(2002)1078-1086正2lt粲能区物理及对加速器和探测实验物理苑长征等高能物理与核物理器设计的要求中心26(2002)1201—120822BES--III主漂移室输出信号的模实验物理王铮等高能物理与核物理拟中心26(2002)1297—130123高速串行数据通信VME插件的实验物理章平等核电子学与探测技术研制中心22(2002)44—4624BESII快速数据重建实验物理荣刚等核电子学与探测技术中心22(2002)105—11025数字式随机脉冲产生器实验物理富洪玉等核电子学与探测技术中心22(2002)162—16526微阴极条感应室实验物理李金等核电子学与探测技术中心22(2002)193—19927BESIII系统环境的网络监测模型实验物理宋立温等核电子学与探测技术中心22(2002)272—27528CMS阴极条室的张力和丝距测实验物理姜春华等核电子学与探测技术量中心22(2002)335—33729北京谱仪PC机物理分析平台介实验物理刘天容等核电子学与探测技术绍中心22(2002)367—37030新型智能CAMAC机箱控制器实验物理张永吉,赵京伟核电子学与探测技术中心等22(2002)382—3843l基于DSP的多通道波形取样电实验物理王铮核电子学与探测技术路设计中心22(2002)409—41132~(3770)扫描实验中利用快速实验物理郭义庆等核电子学与探测技术重建数据测量数据样本的积分亮中心22(2002)420—423度33基于WEB的BES数据存储系统实验物理叶梅等核电子学与探测技术模型中心22(2002)449—45234StudiesofPrototypeCsI(TI)实验物理Y.Liu(刘延)etNIMA482(2002)125 CrystalScin—tillatorsforLow中心a1.EnergyNeutrinoExperiments鬟一附录3.发表论文目录143作者(以原序发表刊物名称序号专着或论文题目单位排列)卷号(年)页35NuclearRecoilMeasurementsin实验物理M.Z.Wang,Phys.Lett.CsI(TbCrystalforColdDark中,J.Li(李金)eta1.B536(2002)203Mat'terDetection36ProbingNeutrinoOscillation实验物理Y.F.Wang(王Phys.ReD65(2002)0730 jointlyinLongandveryLong中心贻芳)etal2lBaselineExperiments37OntheOptimumLongBaseline实验物理Y.F.Wang(王Phys.Rev.D65(2002)0730 f0rtheNextGenerationNeutrino中心贻芳)etaI.06OscillationExperiments38StudyonthePropertyoft天体物理G.M.ChenProceedingsofthethird HadronicDecays中心jointmeetingofChinesephyscistsworldwide,P.185,worldscientific200239Bs.dintechnicolormodel天体物理ZhaohuaPhys.Lett.B546(200)withscalars中心Xiong,JinMin22l-227Y ang40Loopeffectsandnondecoupling天体物理ZhaohuaXiongPhys.Rev.D66, propertyofsupersymmetricQCD中心et.a1.015007(2002)ingb--->tH.4lGreatScintillatingPropertiesofa天体物理GouQuanBuChin.Phys.Lett.V o1.19,Y ALO3:Cecrystal中心et.a1.No.7(2002)92942GreatScintillationgPropertiesofa天体物理苟全朴,李祖物理7期929.930Y A103:CeCrystal中心毫,吕雨生等43非重子暗物质粒子的研究进展天体物理盛祥东,何会物理9期31卷中心林.戴长江577-58044CirX.1的时变性质天体物理屈进禄等河北师大26,1,10中心月45致密星的X射线辐射时延现象天体物理屈进禄,宋黎天文学进展刊物20卷, 中心明,吴枚等第2期143.15546两类长Y暴的里叶功率谱天体物理申荣锋,宋黎明天文43卷第4期中心342-34547L3宇宙线实验触发系统和触发天体物理李忠朝,郁忠高能物理和核物理26. 效率的测量中心强,过雅南等172.17948~掏小型数据获取系统及天体物理何会林,戴长核电子学与探测技术CaF2(Eu)性能的测量中心江,盛祥东22卷第l期27.3049新型"CaF2(Eu)+液闪"复合天体物理盛祥东,戴长高能物理和核物理26 WIMP探测器的实验研究中心江,何会林卷3期273.27850Chandraobservationofsupernova天体物理E.J.Lu.TheAstrophysics568; remnantG54.1+0-3:Aclosecousin中心Q.D.Wang,L49.L52 oftheCRABnebulaL.M.Song,●,●,,,,f,},,};●,,,,,.}●I,I:}::I1,!1,tt●}t{,t,{.^I1.,'l_4l44中国科学院高能物理研究所2002年《年报》作者(以原序发表刊物名称序号专着或论文题目单位排列)卷号(年)页5lBlobejectionfrom天体物理Jian?Min,wangTheApjsadvection.dominatedaccretion中心dengl38:249.263flow.H.themultiwavelengthpropertiesoflightcurves52Comptelobservationsofthe天体物理S.ZhangdengAstronomy386,843?853 gamma-rayblazarpks1622?297中心53KilohertzQuasi—periodic天体物理Wenfei.YuTheAstrophysical osciliationfrequency中心deng567;L67-L70anticorrelatedwithmilihertzquasi—periodicoscillationfluxin4u1608.5254TheAccretionratesandspectral天体物理Jian?MinWangTheApjs energydistributionsofbllacetae中心dengobjects55Bs,d+?inTechnicolor天体物理ZhaohuaPhysicslettersb546 modelwithSCalarS中心Xiong,JinMin221.227Jang56Discoveryofa136millisecond天体物理F.J.LudengAstrophysics574:7卜74 radioandX?raypulsarin中心supernovaremnantG54.1+0.357Chandraobsevationofsupemova天体物理FJ.Lu.AstrophysicalremnantG54.1+0.3:aclosecousin中心L..M..song568;L-49一L52 ofthecrabnebula58低轨道空间闪烁探测器异常区开天体物理张承模,梁晓核电子学及探测技术关机设计及控制中心华,徐玉朋等第22卷第6期59Furtheranalysisofdi?gluonfusion理论室DongshengDu,HighEner.Phys.&Nuc1. mechanismforthedecaysofDeshanY angPhys.V.26,1(2002)B—T1'KandGuohuaiZhu60Phenomenologicalanalysisof理论室DongshengDu,Phys.Rev.D65,074001B--,PPdecayswithQCDHaijunGong,(2002)factorizationJunfengSunetal_6lPhenomenologicalanalysisof理论室DongshengDu,Phys.Rev.D65,094025 charmlessdecaysB—}PVwithHaUunGong,(2002),Erratum,ibid. 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Sigma Chemiluminescent Peroxidase Substrate 产品说明书
Chemiluminescent Peroxidase SubstrateProduct Codes CPS-1, CPS-1-30, CPS-1-60,CPS-1-120, and CPS-1-300Storage Temperature 2-8 °CTECHNICAL BULLETINProduct DescriptionSigma’s Chemiluminescent Peroxidase Substrate can be used for the highly sensitive detection of peroxidase labeled material in a variety of Western blotting applications. This substrate is an enhanced luminol product with a stabilized peroxide buffer solution that provides picogram sensitivity with minimal background interference.ComponentsThe Chemiluminescent Peroxidase Substrate is available in 4 package sizes each containing the Chemiluminescent Reagent (Product Code C 9107) and the Chemiluminescent Reaction Buffer(Product Code C 9232).Package Size C 9107 C 923230 ml 10 ml 20 ml60 ml 20 ml 40 ml120 ml 40 ml 80 ml300 ml 100 ml 200 ml Precautions and DisclaimerThis product is for laboratory research use only. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Preparation InstructionsPrepare the Working Solution by mixing 1 part of the Chemiluminescent Reagent (Product Code C 9107) with 2 parts of the Chemiluminescent Reaction Buffer (Product Code C 9232). Mix well and protect from light. It is recommended to use 0.043 to 0.125 ml per cm2 of membrane. For extended signal duration, a 1:1 ratio of Chemiluminescent Reagent to Chemiluminescent Reaction Buffer may be used. Storage/StabilityIt is recommended to store the components at 2-8 °C. The components are stable for a minimum of18 months when stored in the original container and protected from light. The Working Solution is stable for several hours at room temperature when protected from light.ProcedureSigma’s Chemiluminescent Peroxidase Substrate is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, etc). In a Western blot, an optimized system is needed to minimize background reactivity associated with nonspecific immunochemical interactions. The following is a general guideline for the use of this product. The protocol starts with a transferred membrane.Notes:• For optimal results, individual assay components must be optimized for minimal background andmaximal signal.• This product is designed for use only in Western blotting.• All steps below should be performed with slight agitation on a rocker or an orbital shaker such that the membrane is freely floating.• All incubations should be performed at room temperature.• Gloves must be worn when working with the membrane to avoid contamination.• Azide inhibits horseradish peroxidase (HRP) and should not be used as a buffer preservative forassay components.21. Remove membrane from Western blottingapparatus and wash membrane for 1 minute ineither Tris-buffered Saline with TWEEN 20 (TBST, Product Code T 9039) or phosphate buffered saline with TWEEN 20 (PBST, Product Code P 3563).Note that either a TBS or PBS system can be used for Western blotting.2. Block membrane in appropriate blocking agent for30 minutes. Western Blocker Solution (ProductCode W 0138) is recommended for high sensitivity detection.3. Add primary antibody to the blocking agent. Thefinal concentration of primary antibody in thissolution can range from 0.2-20 µg/ml.4. Incubate membrane with the primary antibodysolution for at least 30 minutes.5. Wash with TBST or PBST for 1 minute.6. Remove TBST or PBST and add at least 10 ml ofappropriate blocking agent to the membrane. Add secondary antibody; a 1:50,000 to 1:500,000dilution in blocking agent may be used.7. Incubate the membrane with the secondaryantibody solution for 30 minutes.8. Remove blocking solution and wash membrane5 times for 5 minutes each with TBST or PBST. 9. Remove the membrane from the wash buffer anddrain any excess liquid from the membrane. Keep the membrane damp; do not let the membrane dry out.10. Place the membrane on a flat sheet of plastic wrap(or on any clean plastic surface).11. Develop the blots with the Working Solution for5 minutes.12. Drain excess substrate and place in holder orplastic wrap.13. Expose BioMax light film to the blot. Exposuretimes range from 30 seconds to 10 minutes. It isbest to do a quick exposure of 10 to 30 seconds to determine what exposure time is needed. If thesignal is too intense even at the short exposuretimes, let the signal decay from 1 to 8 hours andthen re-expose the film. Related ProductsProduct Name Package Size Product Code TBS 10 packets T 6664 PBS 10 packets P 3813 Western BlockerSolution400 ml W 0138 TBS + 3% milk 10 packets T 8793 PBS + 3% milk 10 packets P 2194 PBS + 5% milk 10 packets P 4739 TBS + TWEEN 20 10 packets T 9039 PBS + TWEEN 20 10 packets P 3563Anti-Mouse HRPAntibody2 ml A 9044 TWEEN is a registered trademark of the ICI Group.RBG/MKS/MAM 1/043Troubleshooting Guide Problem TypeCause SolutionNot enough wash steps were performed at the end of the blotting.Double the number of washing steps. Too much primary antibody used. Lower the amount of primary antibody usedand wash with TBST for 5 minutes instead of 1 minute after the primary antibody incubation.Too much background signal observed.Too much secondary antibody used. Lower the amount of secondary antibodyused.Image is reversed on film (dark background and light bands).Too much secondary antibody used. Lower the amount of secondary antibody used.Bands on membrane have brown or yellow tone.Too much secondary antibody used. Lower the amount of secondary antibody used.Too much primary antibody used. Lower the amount of primary antibody used and wash with TBST for 5 minutes instead of 1 minute after the primary antibody incubation.Nonspecific bands show up on membrane.Too much secondary antibody used. Lower the amount of secondary antibodyused.Membrane is stippled. Secondary antibody has some aggregate formation.Filter secondary antibody.Protein levels are too low for detection. Increase exposure time of film and increase level of protein loads. Not enough primary antibody used. Use more primary antibody.No signal is seen with chemiluminescent reaction on membrane. Not enough secondary antibody used. Use more secondary antibody.Sigma brand products are sold through Sigma-Aldrich, Inc.Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side ofthe invoice or packing slip.。
精益西格玛英文缩略语
Lean Sigma Glossary / Abbreviation ListAcronym Definition Descriptionα Alpha The maximum risk or probability ofmaking a Type I Error. This probabilityis always greater than zero and is usuallyestablished at 5%. The researcher makesthe decisions to the greatest level of riskthat is acceptable for a rejection of Ho. β Beta The risk or probability of making a TypeII Error, or overlooking an effectivetreatment or solution to the problem.μ Population mean Symbol used to describe the average foran entire populationσ Population standard deviation Symbol used for standard deviation ofentire population. Measure of thespread of the process (width of thedistribution).% R & R Gage % Repeatability and Reproducibility Addresses what percent of the TotalVariation is taken up by measurementerror.Affinity Diagram A management tool that assists withgeneral planning. It makes disparatelanguage information understandable byplacing it on cards and grouping the cardstogether in a creative manner. "Header"cards are used to summarize each group. ANOVA Analysis of Variance Hypothesis test used to analyze thedifference in means between two or moresamples.Assessment A systematic process of collecting andanalyzing data to determine the current,historical or projected status of anorganization.Assignable Cause The name for the source of variation in aprocess that is not due to chance andtherefore can be identified andeliminated.Audit "The inspection and examination of aprocess or quality system to ensurecompliance to requirements. Audit canapply to an entire organization or bespecific to a function or production step."(Dr. Joseph M. Juran)Baseline Measurement A beginning point based on an evaluationof the output over a period of time todetermine how the process performsprior to any improvement effort.Benchmarking The process of finding and adapting bestperformance.Best Practice A superior method or innovative practicethat contributes to improvedperformance.BB Black Belt A process improvement project teamleader who is trained and certified in theSix Sigma breakthrough methodologyand tools and who is responsible forproject execution.Boxplot "A graphic summary of a distributionwhere the overall dispersion and thecentral tendency or mean of the data arehighlighted." (Arturo Onnias)Brainstorming An idea-generating technique that usesgroup interaction to generate many ideasin a short time period. Ideas are solicitedin a non-judgmental, unrestrictedmanner from all members of a group.Designates a process step that thebusiness requires to stay in business.Capability The total range of inherent variation in astable process. It is determined usingdata from control charts.Capability index A calculated value used to compareprocess variation to a specification.Examples are Cp, Cpk. Can also be usedto compare processes to each other.Cause An established reason for the existence ofa defect or a problem.C/E or C&E Cause and Effect Matrix Prioritization matrix used to assist infocusing in on the root causes of processproblems.Central Tendency The tendency of data gathered from aprocess to cluster toward a middle value,somewhere between the high and lowvalues of measurement.Champion An upper level business leader whofacilitates the leadership,implementation, and deployment of theprocess quality initiative andbreakthrough philosophies.Charter A written commitment by managementstating the scope of authority for animprovement group. Resources,including time and money, are specificallyaddressed.Checksheet A form for recording data on which thenumber of occurrences of an event canbe recorded as ticks or checks.Code of Conduct Expectations of behavior mutually agreedupon by a team. (Also called "norms" or"rules of engagement.")Common Cause A source of process variation that isinherent to the process and is common toall the data.CI Confidence Interval An interval that can be said, with X%certainty, to contain the true processmean or standard deviation. “We are95% confident that the true processmean is in this interval.”Conformance The state of meeting and/or exceedingcustomer requirements and expectations.Consensus A state where everyone in the groupsupports an action or decision, even ifsome of them don't fully agree with it.Consensus Decision A decision made after all aspects of anissue, both positive and negative, havebeen reviewed or discussed to the extentthat everyone openly understands,supports and participates in the decision.Control Keeping a process within boundaries;minimizing the variation of a process.Control Chart A problem solving statistical tool thatindicates whether the system is in or outof control, as determined by computedcontrol limits.Control Limits "Defines natural boundaries of a processwithin specified confidence levels" [uppercontrol limit (UCL), and lower control limit(LCL) defined on a control chart]. (J. R.Russell)Cost-benefit Analysis A way to compare the costs and benefitsof plans. Can be used for comparing thefinancial outcomes of different actionsand determining if a particular actionmakes sense financially.COPQ Cost of Poor Quality Cost associated with poor qualityproducts or services. Examples:Product inspection, Sorting, Scrap,Rework, and Field Complaints.Cp Process Capability Index A calculated value used to compareprocess variation to a specification. Canalso be used to compare processes toeach other.CPD Customer Percent Defective The percentage of products or servicesthat are successfully completed on thefirst attempt without requiring remedialaction or rework.process variation to a specification. Canalso be used to compare processes toeach other.Countermeasure Action taken to counter the verified rootcause of a problem.Critical Characteristic A characteristic dependent on thefunctioning of budget constraints,competitive edge and/or customersatisfaction of the product.Critical Dependencies The interrelationships existing within oramong processes that are primary driversof defects or errors in a product orservice.CPIV Critical Process Input Variable The vital few process input variables thathave the greatest effect on the outputvariable(s) of interest. They are called“X’s”, normally 2 – 6 true criticalvariables.different organizational units or functionsthat are part of a team to solve problems,plan, and develop solutions affecting theorganization as a system.CVA Customer Value Added Term used in Value Analysis of a process.Designates a process step that thecustomer is willing to pay for.CT Cycle Time The average time from a unit enteringthe process until it is completed as afinished good.CTQ Critical to Quality A requirement that a customer values isconsidered Critical to Quality. What thecustomer pays for.Defect Any instance or occurrence where theproduct or service fails to meet customerrequirementsDefect Opportunity A type of potential defect on a unit ofthroughput (output) that is important tothe customer (i.e. Specific fields on aform that creates and opportunity forerror that would be important to thecustomer).Defective Any unit with one or more defects. Seealso DefectsDMAIC Define, Measure, Analyze, Improve, Control 5 steps during a Six Sigma projectDMADV or DMADVI Define, Measure, Analyze, Design, Validateor Define, Measure, Analyze, Design, Verify,ImplementNew process design tool. Commonlyused acronym for Design for Six Sigmamethods.DOTWIMP Defects, Overproduction, Transportation, Seven wastesWaiting, Inventory, Motion, ProcessingDPMO Defects Per Million Opportunities The number of defects in every millionopportunities. By taking into accountopportunities for defects, and not justdefects, we can compare processes ofdiffering complexity.DPU Defects Per Unit The number of defects in a unit orproduct. This measure does not takeinto account the number of opportunitiesfor defects, just the number of defects. DOE Design of Experiments An efficient method of experimentationwhich identifies, with minimum testing,factors (key process input variables) andtheir optimum settings that affect themean and variation.Empowerment Act of placing accountability, authority,and responsibility for processes andproducts at the lowest possible level.Whether or not a person is trulyempowered depends on their acceptanceof responsibility and accountability, theircapabilities, and the seriousness of theconsequences.ENT Entitlement The best a process measure could be.External Failure Nonconformance identified by externalcustomers.Y = F(x1, x2, x3, ……)F Function relating process inputs to processoutputsFMEA Failure Modes and Effects Analysis Method used in measure to understandrisks of failure in a process.quality troubles and restoring the statusquo." (Dr. Joseph M. Juran)FPY First Pass Yield The percentage of products or servicesthat are successfully completed on thefirst attempt without requiring remedialaction or rework.Five “whys” A technique for discovering the rootcause(s) of a problem and showing therelationship of causes by repeatedlyasking the question "Why?"Gage bias (Accuracy) The difference between the true orreference value and the observedaverage of multiple measurements of theidentical characteristic on the same part.Gage repeatability The variation in measurements obtainedwith one measurement instrument whenmeasuring the identical characteristic onthe same part.Gage reproducibility The variation in the average of themeasurements made by differentappraisers using the same measuringinstrument when measuring the identicalcharacteristic on the same part.Gap Analysis The comparison of a current condition tothe desired state.Goal "A broad statement describing a desiredfuture condition or achievement withoutbeing specific about how much andwhen." (Government Performance andResults Act of 1993)GB Green Belt A part-time process improvement projectteam leader who is trained and certifiedin the Six Sigma breakthroughmethodology and tools and who isresponsible for project execution.Typically works on smaller scopedprojects than B B’s.H o Null Hypothesis A statement of no change or difference.This statement is assumed true untilsufficient evidence is presented to rejectit. “If p is low, Ho has to go”aThis statement is considered true if Ho isrejected.Hawthorne effect Every change results (initially, at least) inincreased productivity. Often occursearly in a project, where processparticipants realize the process is understudy.Histogram A graphic way of summarizing data byplotting possible values on one axis andthe observed frequencies for those valueson the other axis. It helps one visualizethe central tendency and dispersion ofthe data.House of Quality A product-planning matrix developedduring quality function deployment thatshows the relationship of customerrequirements to the means of achievingthese requirements. The matrixindicates the impact each of the meanshas on one another.Implementation A structured approach that addresses alland how) of incorporating improvementsinto the process or system.Improvement The organized creation of beneficialchange; the attainment of unprecedentedlevels of performance. Levels ofimprovement range from incremental tomajor; e.g., "breakthrough"improvement.I/O Input Output Type of process map that includesprocess steps and their inputs andoutputs.Inspection Process of measuring, examining ortesting a product service against somerequirement to identify nonconformancebefore it reaches a customer.Internal failures "Product failures that occur before theproduct is delivered to externalcustomers." (Dr. Joseph M. Juran)documentation of standardizedprocesses.JIT Just In Time A concept where an item is delivered,just-in-time, where and when it isneeded.Kaizen In the business world, The word Kaizenhas been utilized by the Japanese tocharacterize a business strategy involveseveryone in an organization workingtogether to make improvements 'withoutlarge capital investments'Kanban "A communications tool in the'just-in-time' production and controlsystem. A kanban, or signboard, isattached to specific parts in a productionline signifying the delivery of a givenquantity. When all parts have been used,the same sign is returned to its originwhere it becomes an order for more."(Kaizen Institute)KJ Jiro Kawakita Name of a noted Japaneseanthropologist. Created method forsummarizing and characterizing largequantities of anthropological datagathered during his expeditions.KPIV Key Process Input Variable The vital few process input variables thathave the greatest effect on the outputvariable(s) of interest. They are called“X’s”, (normally 2 – 6)are called the “Y’s”, (usually 1). May beprocess performance measures orproduct characteristics.LCL Lower Control Limit Demonstrates lower limit of processexpected variability. Points outside ofcontrol limit should be investigated todetermine assignable cause. Formula is(process sample mean – 3 x samplestandard deviation). 99% of theprocess variation falls within the lowerand upper control limits.Little’s Law CT = CT * THLSL Lower Specification Limit Lowest acceptable value or measurementcustomer is willing to accept. Lowerlimit of customer requirement.MBB Master Black Belt A person who is “expert” in S ix Sigmabreakthrough techniques and projectimplementation. MBBs play a key role intraining and coaching Black Belts.Measurement System The complete process used to obtainmeasurements. It consists of thecollection of operations, procedures,gages and other equipment, software,and personnel used to assign a numberor value to the characteristic beingmeasured.MSA Measurement System Analysis Method to understand the capability ofthe measurement system.time that communicates vital informationabout a process or activity. A metricshould drive appropriate leadership ormanagement action. Physically, a metricpackage consists of an operationaldefinition, measurement over time andpresentation.assist in data analysis.Multi-Vari Type of multiple variable process study Assists in identifying the primary x’s thatimpact your Y’s. A graphic way ofdepicting variation within a single entity,machine or process, or between entities(produced at the same time or overtime). Allows the study of processinputs and outputs in a passive mode(natural day-to-day process).Multi-vari Chart A graphic way of depicting variationwithin a single part, machine or process,or between parts (produced at the sameprocess inputs and outputs in a passivemode (natural day-to-day process).Multivoting A structured voting process used toreduce a large number of items, usuallyideas, to a more manageable number forfurther processing or analysis.NGT Nominal Group Technique A tool for generating a list of ideas oropportunities (allows individuals toexpress their opinion). Voting andranking determine priorities.NVA Non-Value Added Term used in Value Analysis of a process.Designates a process step that is notrequired by the business or the customer.Normal distribution A continuous, symmetrical, bell shapedfrequency distribution for variable data.Out of control Describes a process that has variationsthat fluctuate outside the computedcontrol limits. This condition normallyindicates the process is not operating asdesired or that external factors have beenintroduced. A process "out of control" isnot stable and therefore is notpredictable.p p-value Test statistic used in Hypothesis testing.“If p is low, Ho has to go.”P/T Precision to customer tolerance ratio Addresses what percent of the toleranceis taken up by measurement error.Includes both repeatability andreproducibility.Paradigm A set of rules and regulations that definesboundaries and tells what to do to besuccessful within these boundaries.Pareto Chart A statistical method of measurement toidentify the most important problemsthrough different measurement scales;e.g., frequency, cost, etc. It directsattention and efforts to the mostsignificant problems.PPM Parts Per Million Same as DPMO.PDSA Plan Do Study Act Plan-Do-Study-Act: a structured, cyclicalmethodology for developing andimplementing actions of any type: Planfor the action by collecting and analyzingdata and developing alternatives; Do,implement the selected alternative(preferably on a small scale); Study,evaluate results and compare expectedvalues; Act, standardize action and/orWheel)Prevention A quality assurance strategy thatattempts to identify and correctunacceptable service or productcharacteristics during the design,development or production phases.Probability The chance of an event happening or acondition occurring in a random trial.Process The combination of people, equipment,materials, methods, and environmentthat produce output – a given product orservice. It is the particular way of doingsomething.Process map A step-by-step pictorial sequence of aprocess showing process inputs, processoutputs, cycle time rework operations,and inspection points.referred to as the customerrequirem ents. They are called the “Y’s”,(usually 1). May be processperformance measures or productcharacteristics.Process Spread The extent to which the distribution ofindividual values of the processcharacteristic (input or output variable)vary; often shown as the process averageplus and minus some number of standarddeviations. Other related measures ofspread include the range, and variance. QA Quality Assurance Used to describe either a company’squality methods or quality department.Assurance is typically more proactive.Quality Audit "A systematic, independent examinationand review to determine whether qualityactivities and related results comply withplanned arrangements and whether thearrangements are implementedeffectively and are suitable to achieve theobjectives." (ASQC Quality Progress, Feb92)QC Quality Control Used to describe either a company’squality methods or quality department.Control is typically more reactive.QFD Quality Function Deployment A system that translates customerrequirements (voice of the customer) intotechnical requirements for each stage ofdevelopment and/or production.and minimum data points in a sample.R & R Repeatability and Reproducibility Repeatability: The variation inmeasurements obtained with onemeasurement instrument when usedseveral times by one appraiser whilemeasuring the identical characteristic onthe same part.Reproducibility: The variation in theaverage of the measurements made bydifferent appraisers using the samemeasuring instrument when measuringthe identical characteristic on the samepart.RSM Response Surface Methods Method for using DOE to optimizeprocess parameter settings. Allows forfine process adjustment for ultimateoptimization.r b Rate of Bottleneck Process bottleneck rate or rate of theworkstation having the highest long termutilization in that process.RPN Risk Priority Number Metric used in FMEA to quantify risk.Calculated by multiplying the Severity ofthe Effect, the probability of Occurrenceof the Failure and the ability to Detect theFailure. RPN = S x O x DRTY Rolled Throughput Yield The multiplication of all the individual firstpass yields of each step of the entireprocess.s Sample Standard Deviation A measure of the average variation of asample (width of the distribution).SBTI Sigma Breakthrough Technologies, Inc. A consulting firm that specializes in SixSigma implementation.SMED Single Minute Exchange of DieSIPOC SIPOC Map - Suppliers Inputs ProcessOutputs Customers Single page process map that shows the scope of the process your project is examining.SD Standard Deviation A measure of the spread of the process(width of the distribution).SOP Standard Operating Procedure Standardized procedure that isdocumented and used by all processparticipants.SPACER Safety, Purpose, Agenda, Code of Conduct,Expectations and Roles Meeting effectiveness technique. Every meeting should begin with review of safety procedures, meeting purpose, agenda, meeting code of conduct, meeting expectations and meeting roles.Spec Specification The customer requirement for judgingacceptability of a particular characteristic.or s Standard deviation A measure of the spread of the process(width of the distribution).Statistical control The condition describing a process fromwhich all special/assignable causes ofvariation have been eliminated and onlycommon/random causes remain.Applies to both the mean (location) andstandard deviation (spread).SPC Statistical Process Control Method that uses control charting tomanage a process as it occurs.Storyboard Technique to graphically display themethodology used and progress made bya process action team; a board,specifically designated to displayinformationTakt time The pace of the process required to meetcustomer requirements. (Average worktime / Average Demand).Tampering The process of adjusting a stable processto try to compensate for a result that isundesirable or for a result that is extragood, the output that follows will beworse than if the tamperer had left theprocess alone.TOC Theory of Constraints A management philosophy that focusesthe organizations scarce resources onimproving the performance of the trueconstraint, and therefore the bottom lineof the organization.TCS Total Customer SatisfactionT, TH T o Throughput The average output of a productionprocess per unit of time.Average time it takes a single unit totraverse an empty production processprocess variability. Points outside ofcontrol limit should be investigated todetermine assignable cause. Formula is(process sample mean + 3 *s). 99% ofthe process variation falls within thelower and upper control limits.measurement customer is willing toaccept. Upper limit of customerrequirement.U Utilization Utilization of any workstation, capital,process or line is usually defined aspercent of time running divided byVA Value Added Term used in Value Analysis of a process.Designates a process step that is deemedvaluable either by the business or thecustomer.VSM Value Stream Map Type of process map that shows productand information flow. Also showsprocess data.Variation Difference between individualmeasurements. Differences areattributed to common and/or specialcauses.VOC Voice of the Customer Customer requirementsWIP W o Work In Process The inventory between the start and endpoints of a production process or series ofprocesses necessary to produce afinished good.WIP level for which the process achievesmaximum throughput.X Process Input variable In a Six Sigma project, we determine theinputs (X’s) that impact our processoutputs (Y’s).or X-bar Sample mean Symbol used to describe the average of apopulation sample.Y Process Output variable In a Six Sigma project, we determine theinputs (X’s) that impact our processoutputs (Y’s).。
火焰原子吸收光谱法测定土壤中速效钾
火焰原子吸收光谱法测定土壤中速效钾发表时间:2017-12-11T16:08:17.823Z 来源:《基层建设》2017年第24期作者:吴新华[导读] 对提取液进行稀释50倍后,钾在0~2mg/L范围内呈良好的线性关系,相关系数为0.9991,回收率在94.8%~104.6%范围内,相对标准偏差为2.8%,具有很好的精密度和准确性,该方法可满足现代农业工作中土壤中速效钾的检测工作。
惠东县农产品质量安全监督检测中心广东惠州 516300摘要:使用火焰原子吸收光谱法测定土壤中速效钾的含量。
为降低土壤基质对检测结果的影响,通过对乙酸铵提取液进行稀释后检测,结果表明:对提取液进行稀释50倍后,钾在0~2mg/L范围内呈良好的线性关系,相关系数为0.9991,回收率在94.8%~104.6%范围内,相对标准偏差为2.8%,具有很好的精密度和准确性,该方法可满足现代农业工作中土壤中速效钾的检测工作。
关键词:速效钾;土壤Determination Of Soil Exchangeable Potassium By Flame Atomic Absorption Spectrometry(FAAS) Wu Xin-hua1,Feng He-song2(Agricultural Products Quality-safety Supervison and Inspection Center of Huidong;Agro-Technology Extension Center of Huidong,Guangdong Huizhou,516300)Abstract:The contents of available K in soil were determined by flame atomic absorption spectrometry.in order to eliminate the ionization interference of potassium,The ammonium acetate extract was diluted and tested,The results showed that after dilution of the extract 50 times,the method has good precision and accuracy. The linear range was 0~2mg/L,the correlation coefficient was 0.9991. The recoveries were between 94.8%~104.6%,and the relative standard deviation(RSD)was 2.8%. The test results demonstrated that this analytical method can meet the requirement for determination of exchangeable potassium in soil. Keywords:potassium;soil引言钾是农作物生长的主要营养元素,具有增强光合作用和光合产物的运输、激活酶的活性、促进糖和脂的代谢、促进蛋白质合成等作用[1]。
The Refractive Index of Curved Spacetime the Fate of Causality in QED
a r X i v :0707.2303v 2 [h e p -t h ] 29 O c t 2007Preprint typeset in JHEP style -HYPER VERSIONTimothy J.Hollowood and Graham M.Shore Department of Physics,University of Wales Swansea,Swansea,SA28PP,UK.E-mail:t.hollowood@,g.m.shore@ Abstract:It has been known for a long time that vacuum polarization in QED leads to a superluminal low-frequency phase velocity for light propagating in curved spacetime.Assuming the validity of the Kramers-Kronig dispersion relation,this would imply a superluminal wavefront velocity and the violation of causality.Here,we calculate for the first time the full frequency dependence of the refractive index using world-line sigma model techniques together with the Penrose plane wave limit of spacetime in the neighbourhood of a null geodesic.We find that the high-frequency limit of the phase velocity (i.e.the wavefront velocity)is always equal to c andcausality is assured.However,the Kramers-Kronig dispersion relation is violated due to a non-analyticity of the refractive index in the upper-half complex plane,whose origin may be traced to the generic focusing property of null geodesic congruences and the existence of conjugate points.This puts into question the issue of micro-causality,i.e.the vanishing of commutators of field operators at spacelike separated points,in local quantum field theory in curved spacetime.1.IntroductionQuantumfield theory in curved spacetime is by now a well-understood subject.How-ever,there remain a number of intriguing puzzles which hint at deeper conceptual implications for quantum gravity itself.The best known is of course Hawking radia-tion and the issue of entropy and holography in quantum black hole physics.A less well-known effect is the discovery by Drummond and Hathrell[1]that vacuum po-larization in QED can induce a superluminal phase velocity for photons propagating in a non-dynamical,curved spacetime.The essential idea is illustrated in Figure1. Due to vacuum polarization,the photon may be pictured as an electron-positron pair, characterized by a length scaleλc=m−1,the Compton wavelength of the electron. When the curvature scale becomes comparable toλc,the photon dispersion relation is modified.The remarkable feature,however,is that this modification can induce a superluminal1low-frequency phase velocity,i.e.the photon momentum becomes spacelike.Figure1:Photons propagating in curved spacetime feel the curvature in the neighbourhood of their geodesic because they can become virtual e+e−pairs.Atfirst,it appears that this must be incompatible with causality.However, as discussed in refs.[2–4],the relation of causality with the“speed of light”is far more subtle.For our purposes,we may provisionally consider causality to be the requirement that no signal may travel faster than the fundamental constant c defining local Lorentz invariance.More precisely,we require that the wavefront velocity v wf, defined as the speed of propagation of a sharp-fronted wave pulse,should be less than,or equal to,c.Importantly,it may be shown[2,4,5]that v wf=v ph(∞),the high-frequency limit of the phase velocity.In other words,causality is safe even if the low-frequency2phase velocity v ph(0)is superluminal provided the high-frequency limit does not exceed c.This appears to remove the potential paradox associated with a superluminal v ph(0).However,a crucial constraint is imposed by the Kramers-Kronig dispersion relation3(see,e.g.ref.[6],chpt.10.8)for the refractive index,viz.Re n(∞)−Re n(0)=−2ωIm n(ω).(1.1)where Re n(ω)=1/v ph(ω).The positivity of Im n(ω),which is true for an absorptive medium and is more generally a consequence of unitarity in QFT,then implies that Re n(∞)<Re n(0),i.e.v ph(∞)>v ph(0).So,given the validity of the KK dispersion relation,a superluminal v ph(0)would imply a superluminal wavefront velocity v wf= v ph(∞)with the consequent violation of causality.We are therefore left with three main options[4],each of which would have dramatic consequences for our established ideas about quantumfield theory: Option(1)The wavefront speed of light v wf>1and the physical lightcones lie outside the geometric null cones of the curved spacetime,inapparent violation of causality.It should be noted,however,that while this would certainly violate causality for theories in Minkowski spacetime,it could still be possible for causality to be preserved in curved spacetime if the effective metric characterizing the physical light cones defined by v wf nevertheless allow the existence of a global timelike Killing vectorfield. This possible loophole exploits the general relativity notion of“stable causality”[8,9] and is discussed further in ref.[2].Option(2)Curved spacetime may behave as an optical medium ex-hibiting gain,i.e.Im n(ω)<0.This possibility was explored in the context ofΛ-systems in atomic physics in ref.[4], where laser-atom interactions can induce gain,giving rise to a negative Im n(ω)and superluminal low-frequency phase velocities while preserving v wf=1and the KKdispersion relation.However,the problem in extending this idea to QFT is that the optical theorem,itself a consequence of unitarity,identifies the imaginary part of forward scattering amplitudes with the total cross section.Here,Im n(ω)should be proportional to the cross section for e+e−pair creation and therefore positive.A negative Im n(ω)would appear to violate unitarity.Option(3)The Kramers-Kronig dispersion relation(1.1)is itself vio-lated.Note,however,that this relation only relies on the analyticity ofn(ω)in the upper-half plane,which is usually considered to be a directconsequence of an apparently fundamental axiom of local quantumfieldtheory,viz.micro-causality.Micro-causality in QFT is the requirement that the expectation value of the com-mutator offield operators 0|[A(x),A(y)]|0 vanishes when x and y are spacelike separated.While this appears to be a clear statement of what we would understand by causality at the quantum level,in fact its primary rˆo le in conventional QFT is as a necessary condition for Lorentz invariance of the S-matrix(see e.g.ref.[6], chpts.5.1,3.5).Since QFT in curved spacetime is only locally,and not globally, Lorentz invariant,it is just possible there is a loophole here allowing violation of micro-causality in curved spacetime QFT.Despite these various caveats,unitarity,micro-causality,the identification of light cones with geometric null cones and causality itself are all such fundamental elements of local relativistic QFT that any one of these options would represent a major surprise and pose a severe challenge to established wisdom.Nonetheless,it appears that at least one has to be true.To understand how QED in curved spacetime is reconciled with causality,it is therefore necessary to perform an explicit calculation to determine the full frequency dependence of the refractive index n(ω)in curved spacetime.This is the technical problem which we solve in this paper.The remarkable result is that QED chooses option(3),viz.analyticity is violated in curved spacetime.Wefind that in the high-frequency limit,the phase velocity always approaches c,so we determine v wf= 1.Moreover,we are able to confirm that where the background gravitationalfield induces pair creation,γ→e+e−,Im n(ω)is indeed positive as required by unitarity. However,the refractive index n(ω)is not analytic in the upper half-plane,and the KK dispersion relation is modified accordingly.One might think that this implies a violation of microcausality,however,there is a caveat in this line of argument which requires a more ambitious off-shell calculation to settle definitively[7].–3–In order to establish this result,we have had to apply radically new techniques to the analysis of the vacuum polarization for QED in curved spacetime.The original Drummond-Hathrell analysis was based on the low-energy,O(R/m2)effective action for QED in a curved background,L=−1m2 aRFµνFµν+bRµνFµλFνλ+cRµνλρFµνFλρ +···.(1.2) derived using conventional heat-kernel or proper-time techniques(see,for example, [10–14].A geometric optics,or eikonal,analysis applied to this action determines the low-frequency limit of the phase velocity.Depending on the spacetime,the photon trajectory and its polarization,v ph(0)may be superluminal[1,15,16].In subsequent work,the expansion of the effective action to all orders in derivatives,but still at O(R/m2),was evaluated and applied to the photon dispersion relation[11,12,17, 18].However,as emphasized already in refs.[2,3,18],the derivative expansion is inadequate tofind the high-frequency behaviour of the phase velocity.The reason is that the frequencyωappears in the on-shell vacuum polarization tensor only in the dimensionless ratioω2R/m4.The high-frequency limit depends non-perturbatively on this parameter4and so is not accessible to an expansion truncated atfirst order in R/m2.In this paper,we instead use the world-line formalism which can be traced back to Feynman and Schwinger[19,20],and which has been extensively developed in recent years into a powerful tool for computing Green functions in QFT via path integrals for an appropriate1-dim world-line sigma model.(For a review,see e.g.ref.[21].) The power of this technique in the present context is that it enables us to calculate the QED vacuum polarization non-perturbatively in the frequency parameterω2R/m4 using saddle-point techniques.Moreover,the world-line sigma model provides an extremely geometric interpretation of the calculation of the quantum corrections to the vacuum polarization.In particular,we are able to give a very direct interpretation of the origin of the Kramers-Kronig violating poles in n(ω)in terms of the general relativistic theory of null congruences and the relation of geodesic focusing to the Weyl and Ricci curvatures via the Raychoudhuri equations.A further key insight is that to leading order in R/m2,but still exact inω2R/m4, the relevant tidal effects of the curvature on photon propagation are encoded in thef(ωm2Penrose plane-wave limit[22,23]of the spacetime expanded about the original null geodesic traced by the photon.This is a huge simplification,since it reduces the problem of studying photon propagation in an arbitrary background to the much more tractable case of a plane wave.In fact,the Penrose limit is ideally suited to this physical problem.As shown in ref.[24],where the relation with null Fermi normal coordinates is explained,it can be extended into a systematic expansion in a scaling parameter which for our problem is identified as R/m2.The Penrose expansion therefore provides us with a systematic way to go beyond leading order in curvature.The paper is organized as follows.In Section2,we introduce the world-line formalism and set up the geometric sigma model and eikonal approximation.The relation of the Penrose limit to the R/m2expansion is then explained in detail, complemented by a power-counting analysis in the appendix.The geometry of null congruences is introduced in Section3,together with the simplified symmetric plane wave background in which we perform our detailed calculation of the refractive index. This calculation,which is the heart of the paper,is presented in Section4.The interpretation of the result for the refractive index is given in Section5,where we plot the frequency dependence of n(ω)and prove that asymptotically v ph(ω)→1. We also explain exactly how the existence of conjugate points in a null congruence leads to zero modes in the sigma model partition function,which in turn produces the KK-violating poles in n(ω)in the upper half-plane.The implications for micro-causality are described in Section6.Finally,in Section7we make some further remarks on the generality of our results for arbitrary background spacetimes before summarizing our conclusions in Section8.2.The World-Line FormalismFigure2:The loop xµ(τ)with insertions of photon vertex operators atτ1andτ2.–5–In the world-line formalism for scalar QED5the1-loop vacuum polarization is given byΠ1-loop=αT3 T0dτ1dτ2Z V∗ω,ε1[x(τ1)]Vω,ε2[x(τ2)] .(2.1)The loop with the photon insertions is illustrated in Figure(2).The expectation value is calculated in the one-dimensional world-line sigma model involving periodic fields xµ(τ)=xµ(τ+T)with an actionS= T0dτ 15Since all the conceptual issues we address are the same for scalars and spinors,for simplicity we perform explicit calculations for scalar QED in this paper.The generalization of the world-line formalism to spinor QED is straightforward and involves the addition of a further,Grassmann,field in the path integral.For ease of language,we still use the terms electron and positron to describe the scalar particles.6This will require some appropriate iǫprescription.In particular,the T integration contour should lie just below the real axis to ensure that the integral converges at infinity.7In general,one has to introduce ghostfields to take account of the non-trivial measure for the fields, [dxµ(τ)of geometric optics where Aµ(x)is approximated by a rapidly varying exponential times a much more slowly varying polarization.Systematically,we haveAµ(x)= εµ(x)+ω−1Bµ(x)+··· e iωΘ(x).(2.4) We will need the expressions for the leading order piecesΘandε.This will necessitate solving the on-shell conditions to thefirst two non-trivial orders in the expansion in R1/2/ω.To leading order,the wave-vector kµ=ωℓµ,whereℓµ=∂µΘis a null vector (or more properly a null1-form)satisfying the eikonal equation,ℓ·ℓ≡gµν∂µΘ∂νΘ=0.(2.5) A solution of the eikonal equation determines a family or congruence of null geodesics in the following way.9The contravariant vectorfieldℓµ(x)=∂µΘ(x),(2.6) is the tangent vector to the null geodesic in the congruence passing through the point xµ.In the particle interpretation,kµ=ωℓµis the momentum of a photon travelling along the geodesic through that particular point.It will turn out that the behaviour of the congruence will have a crucial rˆo le to play in the resulting behaviour of the refractive index.The general relativistic theory of null congruences is considered in detail in Section3.Now we turn to the polarization vector.To leading order in the WKB approxima-tion,this is simply orthogonal toℓ,i.e.ε·ℓ=0.Notice that this does not determine the overall normalization ofε,the scalar amplitude,which will be a space-dependent function in general.It is useful to splitεµ=Aˆεµ,whereˆεµis unit normalized.At the next order,the WKB approximation requires thatˆεµis parallel transported along the geodesics:ℓ·Dˆεµ=0.(2.7) The remaining part,the scalar amplitude A,satisfies1ℓ·D log A=−εµD·ℓ.(2.9)2Since the polarization vector is defined up to an additive amount of k,there are two linearly independent polarizationsεi(x),i=1,2.Since there are two polarization states,the one-loop vacuum polarization is ac-tually a2×2matrixΠ1-loop ij =αT3 T0dτ1dτ2Z× εi[x(τ1)]·˙x(τ1)e−iωΘ[x(τ1)]εj[x(τ2)]·˙x(τ2)e iωΘ[x(τ2)] .(2.10)In order for this to be properly defined we must specify how to deal with the zero mode of xµ(τ)in the world-line sigma model.Two distinct–but ultimately equiv-alent–methods for dealing with the zero mode have been proposed in the litera-ture[25–29].In thefirst,the position of one particular point on the loop is defined as the zero mode,while in the other,the“string inspired”definition,the zero mode is defined as the average position of the loop:xµ0=1Now notice that the exponential pieces of the vertex operators in(2.1)act as source terms and so the complete action including these ism2S=−T+can always be brought into the formds2=2du dΘ−C(u,Θ,Y a)dΘ2−2C a(u,Θ,Y b)dY a dΘ−C ab(u,Θ,Y c)dY a dY b.(2.14) It is manifest that dΘis a null1-form.The null congruence has a simple description as the curves(u,Θ0,Y a0)forfixed values of the transverse coordinates(Θ0,Y a0).The geodesicγis the particular member(u,0,0,0).It should not be surprising that the Rosen coordinates are singular at the caustics of the congruence.These are points where members of the congruence intersect and will be described in detail in the next section.With the form(2.14)of the metric,onefinds that the classical equations of motion of the sigma model action(2.13)have a solution with Y a=Θ=0whereu(τ)satisfies¨u=−2ωTm2δ(τ).(2.15)More general solutions with constant but non-vanishing(Θ,Y a)are ruled out by the constraint(2.12).The solution of(2.15)is˜u(τ)=−u0+ 2ωT(1−ξ)τ/m20≤τ≤ξ2ωTξ(1−τ)/m2ξ≤τ≤1.(2.16)where the constantu0=ωTξ(1−ξ)/m2(2.17) ensures that the constraint(2.12)is satisfied.The solution describes a loop which is squashed down onto the geodesicγas illustrated in Figure(3).The electron and positron have to move with different world-line velocities in order to accommodate the fact that in generalξis not equal to1Now that we have defined the Rosen coordinates and found the classical saddle-point solution,we are in a position to set up the perturbative expansion.The idea is to scale the transverse coordinatesΘand Y i in order to remove the factor of m2/T in front of the action.The affine coordinate u,on the other hand,will be left alone since the classical solution˜u(τ)is by definition of zeroth order in perturbation theory. The appropriate scalings are precisely those needed to define the Penrose limit[22]–in particular we closely follow the discussion in[23].The Penrose limit involvesfirst a boost(u,Θ,Y a)−→(λ−1u,λΘ,Y a),(2.18) whereλ=T1/2/m,and then a uniform re-scaling of the coordinates(u,Θ,Y a)−→(λu,λΘ,λY a).(2.19) As argued above,it is important that the null coordinate along the geodesic u is not affected by the combination of the boost and re-scaling;indeed,overall(u,Θ,Y a)−→(u,λ2Θ,λY a).(2.20) After these re-scalings,the sigma model action(2.13)becomesS=−T+1m2Θ(ξ)+ωT4 10dτ 2˙u˙Θ−C ab(u,0,0)˙Y a˙Y b −ωT m2Θ(0)+···.(2.22) The leading order piece is precisely the Penrose limit of the original metric in Rosen coordinates.Notice that we must keep the source terms because the combination ωT/m2,or more precisely the dimensionless ratioωR1/2/m2,can be large.However, there is a further simplifying feature:once we have shifted the“field”about the clas-sical solution u(τ)→˜u(τ)+u(τ),it is clear that there are no Feynman graphs with-out externalΘlines that involve the vertices∂n u C ab(˜u,0,0)u n˙Y a˙Y b,n≥1;hence, we can simply replace C ab(˜u+u,0,0)consistently with the background expression C ab(˜u,0,0).This means that the resulting sigma model is Gaussian to leading order in R/m2:S(2)=14 10dτC ab(˜u,0,0)˙Y a˙Y b,(2.23)wherefinally we have dropped the˙u˙Θpiece since it is just the same as inflat space and the functional integral is normalized relative toflat space.This means that all the non-trivial curvature dependence lies in the Y a subspace transverse to the geodesic.10It turns out that the Rosen coordinates are actually not the most convenient co-ordinates with which to perform explicit calculations.For this,we prefer Brinkmann coordinates(u,v,y i).To define these,wefirst introduce a“zweibein”in the subspace of the Y a:C ab(u)=δij E i a(u)E j b(u),(2.24) with inverse E a i.This quantity is subject to the condition thatΩij≡dE iadE ia2E a j.(2.29)du2We have introduced these coordinates at the level of the Penrose limit.However, they have a more general definition for an arbitrary metric and geodesic.They are in fact Fermi normal coordinates.These are“normal”in the same sense as the more common Riemann normal coordinates,but in this case they are associated to the geodesic curveγrather than to a single point.This description of Brinkmann coordinates as Fermi normal coordinates and their relation to Rosen coordinates and the Penrose limit is described in detail in ref.[24].In particular,this reference givestheλexpansion of the metric in null Fermi normal coordinates to O(λ2).To O(λ) this isds2=2du dv−R iuju y i y j du2−dy i2+λ −2R uiuv y i v du2−43R uiuj;k y i y j y k du2 +O(λ2),(2.30)which is consistent with(2.28)since R iuju=−h ij for a plane wave.It is worth pointing out that Brinkmann coordinates,unlike Rosen coordinates,are not singular at the caustics of the null congruence.One can say that Fermi normal coordinates (Brinkmann coordinates)are naturally associated to a single geodesicγwhereas Rosen coordinates are naturally associated to a congruence containingγ.In Brinkmann coordinates,the Gaussian action(2.23)for the transverse coordi-nates becomesS(2)=−12m2Ωij y i y j τ=ξ−ωTexplicitly.In doing so,we discover many surprising features of the dispersion relation that will hold in general.The symmetric plane wave metric is given in Brinkmann coordinates by(2.28), with the restriction that h ij is independent of u.This metric is locally symmetric in the sense that the Riemann tensor is covariantly constant,DλRµνρσ=0,and can be realized as a homogeneous space G/H with isometry group G.12With no loss of generality,we can choose a basis for the transverse coordinates in which h ij is diagonal:h ij y i y j=σ21(y1)2+σ22(y2)2.(3.1) The sign of these coefficients plays a crucial role,so we allow theσi themselves to be purely real or purely imaginary.For a general plane-wave metric,the only non-vanishing components of the Rie-mann tensor(up to symmetries)areR uiuj=−h ij(u).(3.2) So for the symmetric plane wave,we have simplyR uu=σ21+σ22,(3.3)R uiui=−σ2iand for the Weyl tensor,1C uiui=−σ2i+12Notice that,contrary to the implication in ref.[4,18],the condition that the Riemann tensor is covariantly constant only implies that the spacetime is locally symmetric,and not necessarily maximally symmetric[13,23].A maximally symmetric space has Rµνρσ=1plane wave background,then explain how the key features are described in the gen-eral theory of null congruences.The geodesic equations for the symmetric plane wave(2.28),(3.1)are:¨u=0,¨v+2˙u2i=1σ2i y i˙y i=0,¨y i+˙u2σ2i y i=0.(3.5)We can therefore take u itself to be the affine parameter and,with the appropriate choice of boundary conditions,define the null congruence in the neighbourhood of, and including,γas:v=Θ−122i=1σi tan(σi u+a i)y i2.(3.9)The tangent vector to the congruence,defined asℓµ=gµν∂νΘ,is therefore ℓ=∂u+1The polarization vectors are orthogonal to this tangent vector,ℓ·εi=0,and are further constrained by(2.9).Solving(2.7)for the normalized polarization(one-form) yields13ˆεi=dy i+σi tan(σi u+a i)y i du.(3.11) The scalar amplitude A is determined by the parallel transport equation(2.8),from which we readilyfind(normalizing so that A(0)=1)A=2i=1 cos(σi u+a i)(3.12)The null congruence in the symmetric plane wave background displays a number of features which play a crucial role in the analysis of the refractive index.They are best exhibited by considering the Raychoudhuri equation,which expresses the behaviour of the congruence in terms of the optical scalars,viz.the expansionˆθ, shearˆσand twistˆω.These are defined in terms of the covariant derivative of the tangent vector as[30]:ˆθ=1112Rµνℓµℓν,Ψ0=Cµρνσℓµℓνmρmσ.14As demonstrated in refs.[31],the effectof vacuum polarization on low-frequency photon propagation is also governed by the two curvature scalarsΦ00andΨ0.Indeed,many interesting results such as the polarization sum rule and horizon theorem[31,32]are due directly to special properties ofΦ00andΨ0.As we now show,they also play a key rˆo le in the world-line formalism in determining the nature of the full dispersion relation.√2R uu=1 2(C u1u1−C u2u2)=1By its definition as a gradientfield,it is clear that D[µℓν]=0so the null con-gruence is twist-freeˆω=0.The remaining Raychoudhuri equations can then be rewritten as∂u(ˆθ+ˆσ)=−(ˆθ+ˆσ)2−Φ00−|Ψ0|,∂u(ˆθ−ˆσ)=−(ˆθ−ˆσ)2−Φ00+|Ψ0|.(3.15) The effect of expansion and shear is easily visualized by the effect on a circular cross-section of the null congruence as the affine parameter u is varied:the expansionˆθgives a uniform expansion whereas the shearˆσproduces a squashing with expansion along one transverse axis and compression along the other.The combinationsˆθ±ˆσtherefore describe the focusing or defocusing of the null rays in the two orthogonal transverse axes.We can therefore divide the symmetric plane wave spacetimes into two classes, depending on the signs ofΦ00±|Ψ0|.A Type I spacetime,whereΦ00±|Ψ0|are both positive,has focusing in both directions,whereas Type II,whereΦ00±Ψ0 have opposite signs,has one focusing and one defocusing direction.Note,however, that there is no“Type III”with both directions defocusing,since the null-energy condition requiresΦ00≥0.For the symmetric plane wave,the focusing or defocusing of the geodesics is controlled byeq.(3.6),y i=Y i cos(σi u+a i).Type I therefore corresponds toσ1and σ2both real,whereas in Type II,σ1is real andσ2is pure imaginary.The behaviour of the congruence in these two cases is illustrated in Figure(4).1y21y2Figure4:(a)Type I null congruence with the special choiceσ1=σ2and a1=a2so that the caustics in both directions coincide as focal points.(b)Type II null congruence showing one focusing and one defocusing direction.To see this explicitly in terms of the Raychoudhuri equations,notefirst that the curvature scalarsΦ00−|Ψ0|=σ21,Φ00+|Ψ0|=σ22are simply the eigenvalues of h ij.The optical scalars areˆθ=−12 σ1tan(σ1u+a1)−σ2tan(σ2u+a2) (3.16)and we easily verify∂uˆθ=ˆθ2−ˆσ2−12(σ21−σ22).(3.17)It is clear that provided the geodesics are complete,those in a focusing direction will eventually cross.In the symmetric plane wave example,with y i=Y i cos(σi u+ a i),these“caustics”occur when the affine parameterσi u=π(n+115This does not necessarily mean that the conjugate points are joined by more than one actual geodesic,only that an infinitesimal deformation ofγter we shall see that the existence of conjugate points relies on the existence of zero modes of a linear problem.Conversely,the existence of a geodesic other thanγjoining p and q does not necessarily mean that p and q are conjugate[8,33].16Whether these deformed geodesics become actual geodesics is the question as to whether they lift from the Penrose limit to the full metric.4.World-line Calculation of the Refractive IndexIn this section,we calculate the vacuum polarization and refractive index explicitly for a symmetric plane wave.As we mentioned at the end of Section 2,the explicit calculations are best performed in Brinkmann coordinates.We will need the expres-sions for Θand εi for the symmetric plane wave background:these are in eqs.(3.9),(3.11)and (3.12).From these,we have the following explicit expression for the vertex operator 17V ω,εi [x µ(τ)]= ˙y i +σi tan(σi ˜u +a i )˙˜u y i 2 j =1 cos(σj ˜u +a j )×exp iω v +1410dτ ˙y i 2−˙˜u 2σ2i yi 2 −ωT σi −det g [x (τ)]which can be exponenti-ated by introducing appropriate ghosts [25–29].However,in Brinkmann coordinatesafter the re-scaling (2.27),det g =−1+O (λ)and so to leading order in R/m 2the determinant factor is simply 1and so plays no rˆo le.The same conclusion would not be true in Rosen coordinates.The y i fluctuations satisfy the eigenvalue equation¨y i+˙˜u 2σ2i y i −2ωT σi 17Notice that at leading order in R/m 2we are at liberty to replace u (τ)by its classical value ˜u (τ).The argument is identical to the one given in Section 2.。
arXiv0712.0473v1[hep-ph]4Dec2007
a r X i v :0712.0473v 1 [h e p -p h ] 4 D e c 2007Frascati Physics Series Vol.XL VI (2007),pp.000-000HADRON07:XII Int.Conf.on Hadron Spectroscopy –Frascati,October 8-13,2007Plenary/Parallel Session pick one THE EFFECT OF ISOSPIN VIOLATION ON SCALAR MESON PRODUCTION C.Hanhart 1,B.Kubis 2,and J.R.Pel´a ez 31Instit¨u t f¨u r Kernphysik (Theorie),Forschungzentrum J¨u lich,D-52425J¨u lich,Germany 2HISKP (Theorie),Universit¨a t Bonn,Nussallee 14-16,D-53115Bonn,Germany.3Departamento de F´ısica Te´o rica II,Universidad Complutense,E-28040Madrid.Spain Abstract We investigate the isospin-violating mixing of the light scalar mesons a 0(980)and f 0(980)within the unitarized chiral approach.Isospin-violating effects are considered to leading order in the quark mass difference and electromag-netism.In this approach both resonances are generated through meson-meson dynamics.Our results provide a description of the mixing phenomenon within a framework consistent with chiral symmetry and unitarity,where these res-onances are not predominantly q ¯q states.We discuss in detail the reactionsJ/Ψ→φS ,where S denotes a suitable pair of pseudo–scalar mesons in the scalar channel,namely π0η,K +K −,and K 0¯K0.In this work predictions for the cross section in the kaon channels are given for the first time with isospin violating effects included.1IntroductionAlthough the light scalar mesons a0(980)and f0(980)have been established as resonances long ago,there is still a heated debate going on in the literature regarding the very nature of these states.Naively one might assign them a conventional q¯q structure,however,at present no quark model is capable of describing both states simultaneously as q¯q states—see,e.g.,Ref.1).On the other hand,as early as1977it was stressed that especially in the scalar channel the interaction of four-quark systems(two quarks,two antiquarks)is attractive2).Some authors have found indications for the existence of compact four-quark states3−5).However,the same short-ranged interaction can also be the kernel to the scattering of pseudoscalars,giving rise to extended four-quark states that one might call hadronic molecules or extraordinary hadrons6−9). Independently,a similar conclusion was found in different approaches10−13).In Refs.14,15)it is stressed that the effective coupling constants of the scalar mesons to the K¯K channel contain the essential structure information. Especially,the larger the molecular component,the larger the residue at the resonance pole,which acquires its maximum value in case of a pure molecule. It should be stressed,however,that this connection can be made rigorous only for stable bound states and if the bound state pole is on thefirst sheet very close to the elastic threshold14).However,if the state of interest is narrow and the inelastic threshold is sufficiently far away,the argument should still hold15).Note that both conditions apply for a0(980)and f0(980).Therefore one should aim at observables that are very sensitive to the effective coupling constants.The resonance signal,as seen in production experiments for both states,is not very useful to determine those couplings,for it turned out to be mainly sensitive to ratios of couplings16).It is therefore important to investigate other observables.When this formalism was applied to the scalar mesons a0(980)and f0(980) it was found from an analysis of a series of reactions17)that the latter is indeed predominantly a K¯K molecule,in line with the results of Refs.7,10,11,13), while the results for the former did not lead to an unambiguous interpretation. This might either point at a prominent non–molecular contribution to the a0 structure,or the a0is not a bound state,but a virtual state.To decide on this issue it is important to collect more information especially on the a0.In Ref.18)it was stressed that the amplitude for the isospin violating a0−f0Figure1:Graphical illustration of the leading contribution to the a0−f0mixing matrix element,driven by the kaon mass differences.transition should be very sensitive to the product of the effective coupling to the two–kaon channels of the f0and the a0.If we assume that the f0is a molecule,its coupling to the K¯K channel isfixed,as discussed above.Then the value for the a0−f0mixing amplitude should be very sensitive to the structure of the a0.The reason why the mixing amplitude of a0and f0is sensitive to the effective couplings is that it gets a prominent contribution from the kaon–loops (see Fig.1).Their isospin violating part is driven by the kaon mass differences giving rise to an effect that can be shown to scale as1This decay was identified as very useful to study isospin violation for scalar mesons in Refs.21,22).Figure2:Graphical illustration of the subleading contribution to the a0−f0 mixing matrix element,driven by isospin-violating vertices,denoted by the crosses.coupling of the f0to kaons and should therefore provide an independent cross check for the size of corrections of order(m d−m u)/m s.In the next section the formalism is briefly reviewed and the results are given.We close with a short summary.2Formalism and ResultsDetails on the formalism are given in Ref.19).Thus,here we will only repeat the essential physics that went into the calculations.In the Standard Model there are two sources of isospin violation present,namely the up–down quark mass difference as well as electromagnetism.Both appear in a well defined form in the corresponding Lagrangian density formulated in terms of the fundamen-tal degrees of freedom,here photons,gluons,and quarks.Chiral perturbation theory23,24)allows for a consistent representation of those terms for a theory describing the interaction of the(pseudo)Goldstone bosons with each other. At leading order in the chiral Lagrangian the only parameter that appears, in addition to the various particle masses,is the pion decay constant.Thus, also the leading effect of isospin violation is predicted.It should be stressed that already at leading order both isospin violating mass differences as well as isospin violating interactions are present.Already in isospin symmetric calculations of meson–meson scattering am-plitudes,when unitarizing the leading chiral Lagrangian,an a priori unknown constant needs to be adjusted to the data.In addition,a few more parameters need to befitted to the production data—in our case they werefixed from a fit to the data on J/Ψ→φπ+π−,K+K−as well as J/Ψ→ωπ+π−25).Once this is done,the isospin violating signals emerge as predictions.Our resultsFigure3:Left:predicted signal for J/Ψ→φπ0η.The solid line shows the result without isospin violation in the production operator(IVPO),while the band shows the effect of its inclusion.Right:predicted signal for J/Ψ→φK¯K. For both kaon channels the solid band,which includes the uncertainties,is the full result,while for the dashed line the same decay amplitude was used in both channels(see text).for the various channels are shown in Fig.3.The predicted signal in theπ0ηchannel was already published in Ref.19).Note that in an isospin symmetric world this reaction would not be allowed,thus any signal is directly propor-tional to the square of an isospin violating amplitude.Based on very general scale arguments one can show that in addition this amplitude is dominated by the isospin violation that emerges from a0−f0mixing26).This was confirmed on the grounds of the current model:thefilled band in the left panel of Fig.3 shows the effect of possible isospin violation in the production operator.The right panel of the samefigure shows the J/Ψdecay with a pair of kaons in the final state.Please note that for these two channels there would be a decay rate also in an isospin symmetric world,however,then both signals would agree. The solid lines show our results for the two channels including the effects of isospin violation as described in Ref.19).In addition,the presence of charged particles in thefinal state called for an additional treatment of soft photons. Here we followed Ref.27).The decay spectrum for J/Ψ→φK+K−,based on an isospin symmetric calculation,is shown in Ref.25).To see how much of the difference in the two kaon channels originates from the different phase space thresholds alone(2m K+−2m K0=8MeV),in thefigure we also show the signals that emerge when the same decay amplitude is used for both channels(dashed lines).For this calculation we took the average of the charged and the neutral kaon amplitudes,corresponding to a formal isospin0combination.Obviously the by far most important difference between the channels is driven by the displacement of the thresholds,the effect of which is further enhanced by the strongly varying amplitudes precisely in this threshold region.The original idea was to extract information on the charge dependence of the couplings of the f0to kaons from a comparison of J/Ψ→φK+K−and J/Ψ→φK0¯K0.However,as can be seen from the figure,the differences between the solid and the dashed lines are too small to be accessible experimentally.Therefore we do not expect data for the kaon channels to be sufficiently sensitive to extract isospin violating effects in the decay amplitudes.3SummaryIn this work we calculated the reactions J/Ψ→φS,where S denotes a suitable pair of pseudo–scalar mesons in the scalar channel,namelyπ0η,K+K−,and K0¯K0.The goal of this study was to gain a better quantitative understanding of the phenomenon of a0−f0mixing,which should eventually reveal important information on the structure of the a0(980).In addition to theπ0ηchannel,the kaon channels including isospin violation were discussed here for thefirst time. We found that,at least within the current model,the impact of the charge dependence of the coupling of the f0to kaons is too small to be deduced from a comparison of the rates for K+K−,and K0¯K0.On the other hand theπ0ηdecay channel appears to be not only very sensitive to the effective coupling constants that encode the structure information but also theoretically under control19).For a discussion of additional background effects see Ref.22).The corresponding measurements can be performed,once BES III is in operation.4AcknowledgmentsThe authors acknowledge partialfinancial support by the EU integrated in-frastructure initiative HADRONPHYSICS PROJECT,under contract RII3-CT-2004-506078. B.K.is supported by the DFG(SFB/TR16).The re-search of J.R.P.is partially funded by Banco Santander/Complutense con-tract PR27/05-13955-BSCH and Spanish CICYT contracts FPA2007-29115-E, FPA2005-02327,BFM2003-00856.References1.C.R.M¨u nz,J.Resag,B.C.Metsch and H.R.Petry,Nucl.Phys.A578,418(1994)[arXiv:nucl-th/9307027].2.R.L.Jaffe,Phys.Rev.D15,267(1977).3.N.N.Achasov,Phys.Atom.Nucl.67,1529(2004)[Yad.Fiz.67,1552(2004)][arXiv:hep-ph/0309118].4.F.Giacosa,Phys.Rev.D74,014028(2006)[arXiv:hep-ph/0605191].5.J.Vijande,F.Fern´a ndez,A.Valcarce and B.Silvestre-Brac,arXiv:hep-ph/0206263.6.R.L.Jaffe and F.E.Low,Phys.Rev.D19,2105(1979).7.J.R.Pel´a ez,Phys.Rev.Lett.92,102001(2004)[arXiv:hep-ph/0309292].8.J.R.Pel´a ez and G.R´ıos,Phys.Rev.Lett.97,242002(2006).[arXiv:hep-ph/0610397].9.R.L.Jaffe,arXiv:hep-ph/0701038.10.J.D.Weinstein and N.Isgur,Phys.Rev.Lett.48,659(1982);Phys.Rev.D27,588(1983);Phys.Rev.D41,2236(1990).11.G.Janssen,B.C.Pearce,K.Holinde and J.Speth,Phys.Rev.D52,2690(1995)[arXiv:nucl-th/9411021].12.E.van Beveren and G.Rupp,Eur.Phys.J.C22,493(2001)[arXiv:hep-ex/0106077].13.J.A.Oller and E.Oset,Nucl.Phys.A620,438(1997)[Erratum-ibid.A652,407(1999)][arXiv:hep-ph/9702314].14.S.Weinberg,Phys.Rev.130,776(1963);Phys.Rev.131,440(1963);Phys.Rev.137,B672(1965).15.V.Baru,J.Haidenbauer,C.Hanhart,Y.Kalashnikova and A.Kudryavt-sev,Phys.Lett.B586,53(2004)[arXiv:hep-ph/0308129].16.V.Baru,J.Haidenbauer, C.Hanhart, A. 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a rXiv:h ep-ph/8225v122Aug2YITP-00-49Contribution of sigma meson pole to K L -K S mass difference ∗K.Terasaki Yukawa Institute for Theoretical Physics,Kyoto University,Kyoto 606-8502,Japan Abstract The hypothesis of σmeson pole dominance in the |∆I |=12rule in these decays [1],and recently revived in connection with the direct CP violation in the K →ππdecays [2].If it is the case,however,the matrix elements, σ|H w |K ,should survive and give a significant contribution to the K L -K S mass difference,∆m K ,where H w is the strangeness changing (|∆S |=1)effective weak Hamiltonian.Dynamical contributions of various hadron states to hadronic processes in which pion(s)take part can be estimated by using a hard pion technique (with PCAC)in the infinite momentum frame (IMF)[3].For later convenience,we review briefly it below.As an example,we consider a decay,B (p )→π1(q )π2(p ′),in the IMF,i.e.,p →∞,and assume that its amplitude M (B →π1π2)can be approximately evaluated at a slightly unphysical point,q →0,i.e.,q 2→0but (p ·q )is finite:M (B →π1π2)≃lim p →∞,q →0M (B →π1π2).(1)In this approximation,the σ→π+π−amplitude is described in terms of the asymptotic matrix element, π−|A π−|σ ,(matrix element of A π−taken between π−and σwith infinite momentum)as M (σ→π+π−)≃√f π π−|A π−|σ ,(2)which has been symmetrized with respect to exchange of π+and π−in the final state since isospin symmetry is always assumed in this note.The asymptotic matrix element, π−|A π−|σ ,is given bylimp→∞,q→0 π−(p′)|Aπ−|σ(p)=(2π)3δ(3)(p−p′) π−|Aπ−|σ√√√m2n−m2K π2|A¯π1|n n|H w|K +(π1↔π2),(9)M(l)S(K→π1π2)=i2fπm2π−m2Kfπm2π−m2K|M (σ)|≫|M ETC |,|M (n =σ)S |,|M (l )S |,|M fact |,(12)unless the amplitudes in the right-hand-side cancel accidentally each other.However,if the σpole contribution dominates K S →ππamplitudes,it may be worried about that its strange partner,κ,also plays a role in the same amplitudes.The κpole amplitude can be obtained in the same way as M (σ)and its ratio to M (σ)is approximately given byM (κ)(K S →π+π−)m 2κ π|H w |κ4πf 2πm 2σ(m 2σ−m 2π)2| π−|A π−|σ |2,(14)andΓ(σ)(K S →π+π−)≃q Km 2K −m 2σ 2| π−|A π−|σ σ|H w |K 0 |2,(15)respectively,where q σand q K are the center-of-mass momenta of the final pions in the corresponding decays.Since the K S →ππmode dominates the decays of K S ,its total width,ΓK S ,is approximately given by ΓK S ≃32Γ(σ)(K S →π+π−)under the σpole dominance hypothesis.The σmeson pole dominance in the K S →ππmeans that the matrix element, σ|H w |K ,exists and its magnitude should be sizable.Therefore,under this hypothesis,the σmeson pole may give a substantial contribution to ∆m K .The formula describing dynamical con-tributions of hadrons to ∆m K has been given in the IMF long time ago [5].Using it,we obtain the following pole contribution of σmeson,∆m (σ)K =−| K L |H w |σ |2ΓK S ≃1q K (m 2σ−m 2K )m 2K −m 2π 2m K2Γ(σ→π+π−)for m σless than the K ¯Kthreshold (≃1GeV).Now we study whether the above σpole dominance in the K S →ππdecays can be realized in consistency with ∆m K .σ(GeV)mσ(GeV) 1.0- 0.130.50Γσ(GeV)Γm 1.0- 0.130.25RR(σ)(σ)0.50.30.40.50.30.4σ(GeV)(a)(b)Fig.I.R (σ)=∆m (σ)/ΓK S for 0.4<m σ<1.0GeV and 0.3<Γσ<0.5GeV.R (σ)is cut at 0.25in (a)and at 0.50in (b)in order not to exceed the esti-mated ππcontinuum contribution R (ππ)and the measured R exp ,respectively,as discussed in the text.It has been known that contribution of S -wave ππintermediate states to ∆m K can occupy about a half [6]of the observed value [7],i.e.,R (ππ)≡∆m (ππ)K ΓK S exp =0.477±0.022.(18)The above ∆m (ππ)K was estimated by using the Muskhelishvili-Omn`e s equation and the mea-sured ππphase shifts,etc.,in which any indication of σmeson was not obviously seen.Therefore,if σexists,its contribution should be included in the above ∆m (ππ)K ,so that we may put loosely the upper limit of the σpole contribution to ∆m K around the above esti-mate of ∆m (ππ)K ,i.e.,∆m (σ)K /ΓK S <0.25,and look for values of m σand Γσto satisfy it since σmeson is still hypothetical,i.e.,its mass and width are still not confirmed.At energieslower than 900MeV,the ππphase shift analyses have excluded any narrow I =0scalar state but a broad one (Γσ∼500MeV)may have a room in the region [7],0.4<m σ<1.2GeV.In fact,various broad candidates of σmeson with different masses (∼500−700MeV),different widths (∼300−600MeV)and different structures have been studied at this workshop [8].R (σ)in Eq.(17)increases rapidly as m σincreases.It is beyond not only the estimated R (ππ)for m σ>0.55GeV but also the measured R exp in Eq.(18)for m σ>0.57GeV andis much larger than the above cuts in the region m 2σ≫m 2K .Therefore,even if κpolecontribution to the K →ππdecays is taken into account,the result,R (σ)≫R exp form 2σ≫m 2K ,is not changed as discussed before.In this way,it is seen that the σmeson pole dominance in the K →ππamplitudes is not compatible with ∆m K if m σ>0.57GeV and 0.3<Γσ<0.5GeV,unless any other contribution cancels ∆m (σ)K .However,the above does not necessarily imply that the σmeson pole dominance is compatible with the K L -K S mass difference if m σ<0.55GeV,since we have so far con-sidered only the long distance effects on the K L -K S mass difference.The short distance contribution from the box diagram [9]which is estimated by using the factorization may saturate the observed (∆m K )exp although it is still ambiguous because of uncertainty of the so-called B K parameter.If it is the case,however,we need some other contribution to cancel the ππcontinuum contribution (including σmeson pole).Possible candidates are pseudo-scalar(PS)-meson poles since the other contributions of multi hadron intermediate states will be small because of their small phase space volumes.The above implies that the matrix elements, P |H w |K ,P =π0,η,η′,···,survive and their sizes are large enough tocancel ∆m (ππ)K .In this case,however, π|H w |K ’s can give large effects on the K →ππamplitudes [10]through Eq.(4)with Eq.(5)and break the σmeson pole dominance.For the K L →γγdecay,it is known that short distance contribution is small [9].To reproduce the observed rate for this decay,we again need contributions of PS-meson poles given by the matrix elements, P |H w |K ’s,with sufficient magnitude,although their con-tributions are sensitive to the η-η′mixing and are not always sufficient.In fact,the above PS-meson matrix elements can approximately reproduce Γ(K L →γγ)exp ,Γ(K →ππ)exp ’s and (∆m K )exp ,simultaneously,with the help of some other contributions (non-factorizable amplitudes with PS-and K ∗-meson poles for the K L →γγdecay,factorized ones for the K →ππdecays and the short distance contribution to the K 0-¯K0mixing,etc.)but without any contribution of σpole [10].Namely,we do not necessarily need the σpole contribution in the K S →ππdecays.As was seen above,it is unlikely that the σmeson pole amplitude dominates the K S →ππ.It will be seen directly by comparing M (σ)(K S →π+π−)with M ETC (K S →π+π−).If the asymptotic matrix elements, π|H w |K ’s,with sufficient magnitude exist and satisfy the |∆I |=1M ETC (K 0S →π+π−)≃2 m 2K −m 2π π+|H w |K + .(19)The mass dependent factor |(m 2K −m 2π)/(m 2σ−m 2K )|from M(σ)can be enhanced only if m σis very close to m K and σis narrow.However,if Γσwere small,| π−|A π−|σ |also would be small.When we smear out the singularity at m σ=m K using the Breit-Wigner form,the size of |(m 2K −m 2π)/(m 2σ−m 2K ) π−|A π−|σ |is at most ≃2for 0.4<m σ<1.0GeV and0.3<Γσ<0.5GeV.However,any narrowσstate around m K is not allowed[7]as mentioned before.Moreover,σdoes not belong to the same ground state asπand K(for example, 3P0of{q¯q}state in the quark model,etc.),so that the matrix elements,| σ|H w|K |,will be much smaller than| π|H w|K |since wave function overlapping betweenσand K mesonstates will be much smaller than that betweenπand K which belong to the same1S0state of{q¯q}.Therefore,it is unlikely that theσmeson pole amplitude dominates the K→ππamplitudes.An amplitude for dynamical hadronic process can be decomposed into(continuum con-tribution)+(Born term).Since M S has been given by a sum of pole amplitudes,M ETC corresponds to the continuum contribution[11].In the present case,M ETC(K S→ππ)will be dominated by contributions of isoscalar S-waveππintermediate states and develop a phase(≃isoscalar S-waveππphase shift at m K)relative to the Born term which is usually taken to be real in the narrow width limit.The estimated phase difference between|∆I|=12amplitudes for the K→ππdecays is close to the measured isoscalar S-waveππphaseshift at m K[12].It suggests that the isoscalar S-waveππcontinuum contribution will be dominant in the K S→ππamplitudes.In summary,we have studied contribution of theσmeson pole to∆m K under the hypothesis thatσmeson pole dominates the K S→ππamplitudes,and have seen that it provides too large contributions to∆m K and that,to cancel out such effects,contributions of pseudo-scalar-meson poles will be needed.We also have discussed,comparing theσmeson pole amplitude with M ETC in the K S→ππamplitudes,that enhancement of theσmeson pole contribution is not sufficient if it is broad.Additionally,a recent analysis in the K→ππdecays within the theoretical framework of non-linearσmodel suggests that theσmeson pole contribution can occupy,at most,about a half of the|∆I|=12amplitude for the K→ππdecays is very unlikely.The author thanks Dr.D.-X.Zhang and Dr.Y.Y.Keum for discussions and comments. 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