Science Verification Results from PMAS

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香精油中农药残留

香精油中农药残留

Determination of Pesticide Minimum Residue Limits in Essential OilsReport No 3A report for the Rural Industries Research andDevelopment CorporationBy Professor R. C. Menary & Ms S. M. GarlandJune 2004RIRDC Publication No 04/023RIRDC Project No UT-23A© 2004 Rural Industries Research and Development Corporation.All rights reserved.ISBN 0642 58733 7ISSN 1440-6845‘Determination of pesticide minimum residue limits in essential oils’, Report No 3Publication No 04/023Project no.UT-23AThe views expressed and the conclusions reached in this publication are those of the author and not necessarily those of persons consulted. RIRDC shall not be responsible in any way whatsoever to any person who relies in whole or in part on the contents of this report.This publication is copyright. However, RIRDC encourages wide dissemination of its research, providing the Corporation is clearly acknowledged. For any other enquiries concerning reproduction, contact the Publications Manager on phone 02 6272 3186.Researcher Contact DetailsProfessor R. C. Menary & Ms S. M. GarlandSchool of Agricultural ScienceUniversity of TasmaniaGPO Box 252-54HobartTasmania 7001AustraliaPhone: (03) 6226 2723Fax: (03) 6226 7609Email: r.menary@.auIn submitting this report, the researcher has agreed to RIRDC publishing this material in its edited form.RIRDC Contact DetailsRural Industries Research and Development CorporationLevel 1, AMA House42 Macquarie StreetBARTON ACT 2600PO Box 4776KINGSTON ACT 2604Phone: 02 6272 4819Fax: 02 6272 5877Email: rirdc@.auWebsite: .auPublished in June 2004Printed on environmentally friendly paper by Canprint.FOREWORDInternational regulatory authorities are standardising the levels of pesticide residues present in products on the world market which are considered acceptable. The analytical methods to be used to confirm residue levels are also being standardised. To constructively participate in these processes, Australia must have a research base capable of constructively contributing to the establishment of methodologies and must be in a position to assess the levels of contamination within our own products.Methods for the analysis for pesticide residues rarely deal with their detection in the matrix of essential oils. This project is designed to develop and validate analytical methods and apply that methodology to monitor pesticide levels in oils produced from commercial harvests. This will provide an overview of the levels of pesticide residues we can expect in our produce when normal pesticide management programs are adhered to.The proposal to produce a manual which deals with the specific problems associated with detection of pesticide residues in essential oils is intended to benefit the essential oil industry throughout Australia and may prove useful to other horticultural products.This report is the third in a series of four project reports presented to RIRDC on this subject. It is accompanied by a technical manual detailing methodologies appropriate to the analysis for pesticide residues in essential oils.This project was part funded from RIRDC Core Funds which are provided by the Australian Government. Funding was also provided by Essential Oils of Tasmania and Natural Plant Extracts Cooperative Society Ltd.This report, an addition to RIRDC’s diverse range of over 1000 research publications, forms part of our Essential Oils and Plant Extracts R&D program, which aims for an Australian essential oils and plant extracts industry that has established international leadership in production, value adding and marketing.Most of our publications are available for viewing, downloading or purchasing online through our website:•downloads at .au/fullreports/index.html•purchases at .au/eshopSimon HearnManaging DirectorRural Industries Research and Development CorporationAcknowledgementsOur gratitude and recognition is extended to Dr. Noel Davies (Central Science Laboratories, University of Tasmania) who provided considerable expertise in establishing procedures for chromatography mass spectrometry.The contribution to extraction methodologies and experimental work-up of Mr Garth Oliver, Research Assistant, cannot be underestimated and we gratefully acknowledge his enthusiasm and novel approaches.Financial and ‘in kind’ support was provided by Essential Oils Industry of Tasmania, (EOT).AbbreviationsADI Average Daily IntakeAGAL Australian Government Analytical Laboratoriesingredientai activeAPCI Atmospheric Pressure Chemical IonisationBAP Best Agricultural PracticesenergyCE collisionDETA DiethylenetriamineECD Electron Capture DetectorionisationESI ElectrosprayFPD Flame Photometric DetectionChromatographyGC GasResolutionHR HighChromatographyLC LiquidLC MSMS Liquid Chromatography with detection monitoring the fragments of Mass Selected ionsMRL Maximum Residue LimitSpectrometryMS MassNRA National Registration AuthorityR.S.D. Relative Standard DeviationSFE Supercritical Fluid ExtractionSIM Single Ion MonitoringSPE Solid Phase ExtractionTIC Total Ion ChromatogramContents FOREWORD (III)ACKNOWLEDGEMENTS (IV)ABBREVIATIONS (V)CONTENTS (VI)EXECUTIVE SUMMARY (VII)1. INTRODUCTION (1)1.1B ACKGROUND TO THE P ROJECT (1)1.2O BJECTIVES (2)1.3M ETHODOLOGY (2)2. EXPERIMENTAL PROTOCOLS & DETAILED RESULTS (3)2.1M ETHOD D EVELOPMENT (3)2.2M ONITORING OF H ARVESTS (42)2.3P RODUCTION OF M ANUAL (46)3. CONCLUSIONS (47)IMPLICATIONS & RECOMMENDATIONS (50)BIBLIOGRAPHY (50)Executive SummaryThe main objective of this project was to continue method development for the detection of pesticide residues in essential oils, to apply those methodologies to screen oils produced by major growers in the industry and to produce a manual to consolidate and coordinate the results of the research. Method development focussed on the effectiveness of clean-up techniques, validation of existing techniques, the assessment of the application of gas chromatography (GC) with detection using electron capture detectors (ECD), flame photometric detectors (FPD) and high pressure liquid chromatography (HPLC) with ion trap mass selective (MS) detection.The capacity of disposable C18 cartridges to separate components of boronia oil was found to be limited with the majority of boronia components being eluted on the solvent front, with little to no separation achieved. The cartridges were useful, however, in establishing the likely interaction of reverse phases (RP) C18 columns with components of essential oils, using polar mobile phases . The loading of large amounts of oil onto RP HPLC columns presents the risk of permanently contaminating the bonded phases. The lack of retention of components on disposable SPE C18 cartridges, despite the highly polar mobile phase, presented a good indication that essential oils would not accumulate on HPLC RP columns.The removal of non-polar essential oil components by solvent partitioning of distilled oils was minimal, with the recovery of pesticides equivalent to that recorded for the essential oil components. However application of this technique was of advantage in the analysis of solvent extracted essential oils such as those produced from boronia and blackcurrant.ECD was found to be successful in the detection of terbacil, bromacil, haloxyfop ester, propiconazole, tebuconazole and difenaconzole. However, analysis of pesticide residues in essential oils by application of GC ECD is not sufficiently sensitive to allow for a definitive identification of any contaminant. As a screen, ECD will only be effective in establishing that, in the absence of a peak eluting with the correct retention time, no gross contamination of pesticide residues in an essential oil has occurred . In the situation where a peak is recorded with the correct elution characteristics, and which is enhanced when the sample is fortified with the target analyte, a second means of contaminant identification would be required. ECD, then, can only be used to rule out significant contamination and could not in itself be adequate for a positive identification of pesticide contamination.Benchtop GC daughter, daughter mass spectrometry (MSMS) was assessed and was not considered practical for the detection of pesticide residues within the matrix of essential oils without comprehensive clean-up methodologies. The elution of all components into the mass spectrometer would quickly lead to detector contamination.Method validation for the detection of 6 common pesticides in boronia oil using GC high resolution mass spectrometry was completed. An analytical technique for the detection of monocrotophos in essential oils was developed using LC with detection by MSMS. The methodology included an aqueous extraction step which removed many essential oil components from the sample.Further method development of LC MSMS included the assessment of electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI. For the chemicals trialed, ESI has limited application. No response was recorded for some of the most commonly used pesticides in the essential oil industry, such as linuron, oxyflurofen, and bromacil. Overall, there was very little difference between the sensitivity for ESI and APCI. However, APCI was slightly more sensitive for the commonly used pesticides, tebuconazole and propiconazole, and showed a response, though poor, to linuron and oxyflurofen. In addition, APCI was the preferred ionisation method for the following reasons,♦APCI uses less nitrogen gas compared to ESI, making overnight runs less costly;♦APCI does not have the high back pressure associated with ionisation by ESI such that APCI can be run in conjunction with UV-VIS without risk of fracturing the cell, which is pressure sensitive. Analytes that ionised in the negative APCI mode were incorporated into a separate screen which included bromacil, terbacil, and the esters of the fluazifop and haloxyfop acids. Further work using APCI in the positive mode formed the basis for the inclusion of monocrotophos, pirimicarb, propazine and difenaconazole into the standard screen already established. Acephate, carbaryl, dimethoate, ethofumesate and pendimethalin all required further work for enhanced ionisation and / or improved elution profiles. Negative ionisation mode for APCI gave improved characteristics for dicamba, procymidone, MCPA and mecoprop.The thirteen pesticides included in this general screen were monocrotophos, simazine, cyanazine, pirimicarb, propazine, sethoxydim, prometryb, tebuconazole, propiconazole, , difenoconazole and the esters of fluroxypyr, fluazifop and haloxyfop.. Bromacil and terbacil were not included as both require negative ionisation and elute within the same time window as simazine, which requires positive ionisation. Cycling the MS between the two modes was not practical.The method validation was tested against three oils, peppermint, parsley and fennel.Detection limits ranged from 0.1 to 0.5 mgkg-1 within the matrix of the essential oils, with a linear relationship established between pesticide concentration and peak height (r2 greater than 0.997) and repeatabilities, as described by the relative standard deviation (r.s.d), ranging from 3 to 19%. The type of oil analysed had minimal effect on the response function as expressed by slope of the standard curve.The pesticides which have an carboxylic acid moiety such as fluazifop, haloxyfop and fluroxypyr, present several complications in any analytical method development. The commercial preparations usually have the carboxylic acid in the ester form, which is hydrolysed to the active acidic form on contact with soil and vegetation. In addition, the esters may be present in several forms, such as the ethoxy ethyl or butyl esters. Detection using ESI was tested. Preliminary results indicate that ESI is unsuitable for haloxyfop and fluroxypyr ester. Fluazifop possessed good ionisation characteristics using ESI, with responses approximately thirty times that recorded for haxloyfop. Poor chromatography and response necessitated improved mobile phase and the effect of pH on elution characteristics was considered the most critical parameter. The inclusion of acetic acid improved peak resolution.The LC MSMS method for the detection of dicamba, fluroxypyr, MCPA, mecoprop and haloxyfop in peppermint and fennel distilled oils underwent the validation process. Detection limits ranged from 0.01 to 0.1 mgkg-1Extraction protocols and LC MSMS methods for the detection of paraquat and diquat were developed. ESI produced excellent responses for both paraquat and diquat, after some modifications of the mobile phase. Extraction methodology using aqueous phases were developed. Extraction with carbonate buffer proved to be the most effective in terms of recovery and robustness. A total ion chromatogram of the LC run of an aqueous extract of essential oil was recorded and detection using a photodiode array detector confirmed that very little essential oil matrix was co-extracted. The low background noise indicated that samples could be introduced directly into the MS. This presented a most efficient and rapid way for analysis of paraquat and diquat, avoiding the need for specialised columns or modifiers to be included in the mobile phase to instigate ion exchange.The adsorbtion of paraquat and diquat onto glass and other surfaces was reduced by the inclusion of diethylenetriamine (DETA). DETA preferentially accumulates on the surfaces of sample containers, competitively binding to the adsorption sites. All glassware used in the paraquat diquat analysis were washed in a 5% solution of 0.1M DETA, DETA was included in all standard curve preparations, oils were extracted with aqueous DETA and the mobile phase was changed to 50:50 DETA / methanol. The stainless steel tubing on the switching valve was replaced with teflon, further improvingreproducibility. Method validation was undertaken of the analysis of paraquat and diquat using the protocols established. The relationship between analyte concentration and peak area was not linear at low concentrations, with adsorption more pronounced for paraquat, such that the response for this analyte was half that seen for diquat and the 0.1 mgkg-1 level.The development of a method for the detection of the dithiocarbamate, mancozeb was commenced. Disodium N, N'-ethylenebis(dithiocarbamate) was synthesised as a standard for the derivatised final analytical product. An LC method, with detection using MSMS, was successfully completed. The inclusion of a phase transfer reagent, tetrabutylammonium hyrdrogen sulfate, required in the derivatisation step, contaminated the LC MSMS system, such that any signal from the target analyte was masked. Alternatives to the phase transfer reagent are now being investigated.Monitoring of harvests were undertaken for the years spanning 1998 to 2001. Screens were conducted covering a range of solvent extracted and distilled oils. Residues tested for included tebuconazole, simazine, terbacil, bromacil, sethoxydim, prometryn, oxyflurofen, pirimicarb, difenaconazole, the herbicides with acidic moieties and paraquat and diquat. Problems continued for residues of propiconazole in boronia in the 1998 / 1999 year with levels to 1 mgkg-1 still being detected. Prometryn residues were detected in a large number of samples of parsley oil.Finally the information gleaned over years of research was collated into a manual designed to allow intending analysts to determine methodologies and equipment most suited to the type of the pesticide of interest and the applicability of analytical equipment generally available.1. Introduction1.1 Background to the ProjectResearch undertaken by the Horticultural Research Group at the University of Tasmania, into pesticide residues in essential oils has been ongoing for several years and has dealt with the problems specific to the analysis of residues within the matrix of essential oils. Analytical methods for pesticides have been developed exploiting the high degree of specificity and selectivity afforded by high resolution gas chromatography mass spectrometry. Standard curves, reproducibility and detection limits were established for each. Chemicals, otherwise not amenable to gas chromatography, were derivatised and incorporated into a separate screen to cover pesticides with acidic moieties.Research has been conducted into low resolution GC mass selective detectors (MSD and GC ECD. Low resolution GC MSD achieved detection to levels of 1 mgkg-1 in boronia oil, whilst analysis using GC ECD require a clean-up step to effectively detect halogenated chemicals below 1mgkg-1.Dithane (mancozeb) residues were digested using acidified stannous chloride and the carbon disulphide generated from this reaction analysed by GC coupled to FPD in the sulphur mode.Field trials in peppermint crops were established in accordance with the guidelines published by the National Registration Authority (NRA), monitoring the dissipation of Tilt and Folicur residues in peppermint leaves and the co-distillation of these residues with hydro-distilled peppermint oils were assessed.Development of extraction protocols, analytical methods, harvest monitoring and field trials were continued and were detailed in a subsequent report. Solvent-based extractions and supercritical fluid extraction (SFE) was found to have limited application in the clean-up of essential oilsIn conjunction with Essential Oils of Tasmania (EOT), the contamination risk, associated with the introduction of a range of herbicides, was assessed through a series of field trials. This required analytical method development to detect residues in boronia flowers, leaf and oil. The methodology for a further nine pesticides was successful applied. Detection limits for these chemicals ranged from 0.002 mgkg-1 to 0.1 mgkg-1. In addition, methods were developed to analyse for herbicides with active ingredients (ai) whose structure contained acidic functional groups. Two methods of pesticide application were trialed. Directed sprays refer to those directed on the stems and leaves of weeds at the base of boronia trees throughout the trial plot. Cover sprays were applied over the entire canopy. For all herbicides for which significant residues were detected, it was evident that cover sprays resulted in contamination levels ten times those occurring as a result of directed spraying in some instances. Chloropropham, terbacil and simazine presented potentially serious residue problems, with translocation of the chemical from vegetative material to the flower clearly evident.Directed spray applications of diuron and dimethenamid presented only low residue levels in extracted flowers with adequate control of weeds. Oxyflurofen and the mixture of bromacil and diuron (Krovar) presented only low levels of residues when used as a directed spray and were effective as both post and pre-emergent herbicides. Only very low levels of residues of both sethoxydim and norflurazon were detected in boronia oil produced in crops treated with directed spray applications. Sethoxydim was effective as a cover spray for grasses whilst norflurazon showed potential as herbicide to be used in combination with other chemicals such as diuron, paraquat and diquat. Little contamination of boronia oils by herbicides with acidic moieties was found. This advantage, however, appears to be offset by the relatively poor weed control. Both pendimethalin and haloxyfop showed good weed control. Both, however, present problems with chemical residues in boronia oil and should only be used as a directed sprayThe stability of tebuconazole, monocrotophos and propiconazole in boronia under standard storage conditions was investigated. Field trials of tebuconazole and propiconazole were established in commercial boronia crops and the dissipation of both were monitored over time. The amount of pesticide detected in the oils was related to that originally present in the flowers from which the oils were produced.Experiments were conducted to determine whether the accumulation of terbacil residues in peppermint was retarding plant vigour. The level recorded in the peppermint leaves were comparatively low. Itis unlikely that terbacil carry over is the cause for the lack of vigour in young peppermint plants.Boronia oils produced in 1996, 1997 and 1998 were screened for pesticides using the analytical methods developed. High levels of residues of propiconazole were shown to persist in crops harvested up until 1998. Field trials have shown that propiconazole residues should not present problems if the fungicide is used as recommended by the manufacturers.1.2 Objectives♦Provide the industry, including the Standards Association of Australia Committee CH21, with a concise practical reference, immediately relevant to the Australian essential oil industry♦Facilitate the transfer of technology from a research base to practical application in routine monitoring programs♦Continue the development of analytical methods for the detection of metabolites of the active ingredients of pesticide in essential oils.♦Validate the methods developed.♦Provide industry with data supporting assurances of quality for all exported products.♦Provide a benchmark from which Australia may negotiate the setting of a realistic maximum residue limit (MRL)♦Determine whether the rate of uptake is relative to the concentration of active ingredient on the leaf surface may establish the minimum application rates for effective pest control.1.3 MethodologyThree approaches were used to achieve the objectives set out above.♦Continue the development and validation of analytical methods for the detection of pesticide residues in essential oils. Analytical methods were developed using gas chromatography high resolution mass spectrometry (GC HR MS), GC ECD, GC FPD and high pressure liquid chromatography with detection using MSMS.♦Provide industry with data supporting assurances of quality for all exported products.♦Coordinate research results into a comprehensive manual outlining practical approaches to the development of analytical proceduresOne aspect of the commissioning of this project was to provide a cost effective analytical resource to assess the degree of the pesticide contamination already occurring in the essential oils industry using standard pesticide regimens. Oil samples from annual harvests were analysed for the presence of pesticide residues. Data from preceding years were collated to determine the progress or otherwise, in the application of best agricultural practice (BAP).2. Experimental Protocols & Detailed ResultsThe experimental conditions and results are presented under the following headings:♦Method Development♦Monitoring of Commercial Harvests♦Production of a Manual2.1 Method DevelopmentMethod development focussed on the effectiveness of clean-up techniques, validation of existing techniques, the assessment of the application of GC ECD and FPD and high pressure liquid chromatography with ion trap MS, MS detection.2.1.1 Clean-up Methodologies2.1.1.i. Application of Disposable SPE cartridges in the clean-up of pesticide residues in essentialoilsLiterature reviews provided limited information with regards to the separation of contaminants within essential oils. The retention characteristics of disposable C18 cartridges were trialed.Experiment 1;Aim : To assess the capacity of disposable C18 cartridges to the separation of boronia oil components. Experimental : Boronia concrete (49.8 mg) was dissolved in 0.5 mL of acetone and 0.4 mL of chloroform was added. 1mg of octadecane was added as an internal standard. A C18 Sep-Pak Classic cartridge (short body) was pre- conditioned with 1.25 mL of methanol, which was passed through the column at 7.5 mLmin-1, followed by 1.25 mL of acetone, at the same flow rate. The boronia samplewas then applied to the column at 2 mLmin-1 flow and eluted with 1.25 mL of acetone / chloroform (5/ 4) and then eluted with a further 2.5 mL of chloroform. 5 fractions of 25 drops each were collected. The fractions were analysed by GC FID using the following parametersAnalytical parameters6890PackardHewlettGCcolumn: Hewlett Packard 5MS 30m, i.d 0.32µmcarrier gas instrument grade nitrogeninjection volume: 1µL (split)injector temp: 250°Cdetector temp: 280°Cinital temp: 50°C (3 min), 10°Cmin-1 to 270°C (7 mins)head pressure : 10psi.Results : Table 1 record the percentage volatiles detected in the fractions collectedFraction 1 2 3 4 5 % components eluting 18 67 13 2636%monoterpenes 15%sesquiquiterpenes 33 65 2%high M.W components 1 43 47 9Table 1. Percentage volatiles eluting from SPE C18 cartridgesDiscussion : The majority of boronia components eluted on the solvent front, effecting minimal separation. This area of SPE clean-up of essential oils requires a wide ranging investigation, varying parameters such as cartridge type and polarity of mobile phase.Experiment 2.Aim : For the development of methods using LC MSMS without clean-up steps, the potential for oil components to accumulate on the reverse phase (RP) column must be assessed. The retention of essential oil components on SPE C18 cartridges, using the same mobile phase as that to be used in theLC system, would provide a good indication as to the risk of contamination of the LC columns withoil components.Experimental: Parsley oil (20-30 mg) was weighed into a GC vial. 200 µL of a 10 µgmL-1 solution (equivalent to 100mgkg-1 in oil) of each of sethoxydim, simazine, terbacil, prometryn, tebuconazoleand propiconazole were used to spike the oil, which was then dissolved in 1.0 mL of acetonitrile. The solution was then slowly introduced to the C18 cartridge (Waters Sep Pac 'classic' C18 #51910) using a disposable luer lock, 10 mL syringe, under constant manual pressure, and eluted with 9 mLs of acetonitrile. Ten, 1 mL fractions were collected and transferred to GC vials. 1mg of octadecane was added to each vial and the samples were analysed by GC FID under the conditions described in experiment 1.The experiment was repeated using C18 cartridges which had been pre-conditioned with distilled waterfor 15 mins. Again, parsley oil, spiked with pesticides was eluted with acetonitrile and 5 x 1 mL fractions collected.Results: The majority of oil components and pesticides were eluted from the C18 cartridge in the firsttwo fractions. Little to no separation of the target pesticides from the oil matrix was achieved. Table2 lists the distribution of essential oil components in the fractions collected.Fraction 1 2 3 4 5 % components eluting 18 67 13 2663%monoterpenes 15%sesquiquiterpenes 33 65 2%high M.W components 1 43 47 9water conditioned% components eluting 35 56 8 12%monoterpenes 3068%sesquiquiterpenes 60 39 1 0%high M.W components 0 50 42 7Table 2. Percentage volatiles eluting for SPE C18 cartridgesFigure 1 shows a histogram of the percentage distribution of components from the oil in each of the four fractions.Figure 1. Histogram of the percentage of volatiles of distilled oils in each of four fraction elutedon SPE C18 cartridges (non-preconditioned)Figure 2. Histogram of the percentage of volatiles of distilled oils in each of four fraction elutedon SPE C18 cartridges (preconditioned)Discussion : The chemical properties of many of the target pesticides, including polarity, solubility in organic solvents and chromatographic behaviour, are similar to the majority of essential oil components. This precludes the effective separation of analytes from such matrices through the use of standard techniques, where the major focus is pre-concentration of pesticide residues from water or water based vegetative material. However, this experiment served to provide a good indication that under HPLC conditions, where a reverse phase C18 column is used in conjunction with acetonitrile / water based mobile phases, essential oil components do not remain on the column.。

Appendix H - The Nature of Science in the Next Generation Science Standards 4.15.13

Appendix H - The Nature of Science in the Next Generation Science Standards 4.15.13

APPENDIX H – Understanding the Scientific Enterprise: The Nature of Science inthe Next Generation Science StandardsScientists and science teachers agree that science is a way of explaining the natural world. In common parlance, science is both a set of practices and the historical accumulation of knowledge. An essential part of science education is learning science and engineering practices and developing knowledge of the concepts that are foundational to science disciplines. Further, students should develop an understanding of the enterprise of science as a whole—the wondering, investigating, questioning, data collecting and analyzing. This final statement establishes a connection between the Next Generation Science Standards (NGSS) and the nature of science. Public comments on previous drafts of the NGSS called for more explicit discussion of how students can learn about the nature of science.This chapter presents perspectives, a rationale and research supporting an emphasis on the nature of science in the context of the NGSS. Additionally, eight understandings with appropriate grade-level outcomes are included as extensions of the science and engineering practices and crosscutting concepts, not as a fourth dimension of standards. Finally, we discuss how to emphasize the nature of science in school programs.The Framework for K-12 Science EducationA Framework for K-12 Science Education: Practices, Crosscutting Concepts, and Core Ideas (NRC, 2012) acknowledged the importance of the nature of science in the statement “…there is a strong consensus about characteristics of the scientific enterprise that should be under stood by an educated citizen” (NRC, 2012, page 78). The Framework reflected on the practices of science and returned to the nature of science in the following statement: “Epistemic knowledge is knowledge of the constructs and values that are intrinsic to science. Students need to understand what is meant, for example, by an observation, a hypothesis, an inference, a model, a theory, or a claim and be able to distinguish among them” (NRC, 2012, page 79). This quotation presents a series ofconcepts and activities important to understanding the nature of science as a complement to the practices imbedded in investigations, field studies, and experiments.Nature of Science: A Perspective for the NGSSThe integration of scientific and engineering practices, disciplinary core ideas, and crosscutting concepts sets the stage for teaching and learning about the nature of science. This said, learning about the nature of science requires more than engaging in activities and conducting investigations.When the three dimensions of the science standards are combined, one can ask what is central to the intersection of the scientific and engineering practices, disciplinary core ideas, and crosscutting concepts? Or, what is the relationship among the three basic elements of A Framework for K-12 Science Education? Humans have a need to know and understand the world around them. And they have the need to change their environment using technology in order to accommodate what they understand or desire. In some cases, the need to know originates in satisfying basic needs in the face of potential dangers. Sometimes it is a natural curiosity and, in other cases, the promise of a better, more comfortable life. Science is the pursuit of explanations of the natural world, and technology and engineering are means of accommodating human needs, intellectual curiosity and aspirations.One fundamental goal for K-12 science education is a scientifically literate person who can understand the nature of scientific knowledge. Indeed, the only consistent characteristic of scientific knowledge across the disciplines is that scientific knowledge itself is open to revision in light of new evidence.In K-12 classrooms, the issue is how to explain both the natural world and what constitutes the formation of adequate, evidence-based scientific explanations. To be clear, this perspective complements but is distinct from students engaging in scientific and engineering practices in order to enhance their knowledge and understanding of the natural world.A Rationale and ResearchAddressing the need for students to understand both the concepts and practices of science and the nature of science is not new in American education. The writings of James B. Conant in the 1940s and 50s, for example, argue for a greater understanding of science by citizens (Conant, 1947). In Science and Common Senses (1951), Conant discusses the “bewilderment of laymen” when it comes to understanding what science can and cannot accomplish, both in the detailed context of investigations and larger perspective of understanding science. Conant says: “…The remedy does not lie in a greater dissemination of scientific information among nonscientists. Being well informed about science is not the same thing as understanding science, though the two propositions are not antithetical. What is needed is methods for importing some knowledge of the tactics and strategy of science to those who are not scientists” (Conant, 1951, page 4). In the context of the discussion here, tactics are analogous to science and engineering practices, as well as to the nature of scientific explanations.The present discussion recommends the aforementioned “tactics of science and engineering practices and crosscutting concepts” to develop students’ understanding of the larger strategies of the scientific enterprise—the nature of scientific explanations. One should note that Conant and colleagues went on to develop Harvard Cases in History of Science, a historical approach to understanding science. An extension of the nature of science as a learning goal for education soon followed the original work at Harvard. In the late 1950s, Leo Klopfer adapted the Harvard Cases for use in high schools (Klopfer & Cooley, 1963). Work on the nature of science has continued with lines of research by Lederman (1992), Lederman and colleagues (Lederman et al., 2002), and Duschl (1990; 2000; 2008). One should note that one aspect of this research base addresses the teaching of the nature of science (see, e.g., Lederman & Lederman, 2004; Flick & Lederman, 2004; Duschl, 1990; McComas, 1998; Osborne et al., 2003; Duschl & Grandy, 2008).Further support for teaching about the nature of science can be seen in 40 years of Position Statements from the National Science Teachers Association (NSTA). In the late 1980s, Science for All Americans (Rutherford & Ahlgren, 1989), the 1990s policy statement Benchmarks for Science Literacy (AAAS, 1993), and National ScienceEducation Standards (NRC, 1996) clearly set the understanding of the nature of science as a learning outcome in science education.Recently, discussions of A Framework for K-12 Science Education (NRC, 2012) and implications for teaching science have provided background for instructional strategies that connect specific practices and the nature of scientific explanations (Duschl, 2012; Krajcik & Merritt, 2012; Reiser, Berland, & Kenyon, 2012).The Nature of Science and NGSSThe nature of science is included in the Next Generation Science Standards. Here we present the NOS Matrix. The basic understandings about the nature of science are:▪Scientific Investigations Use a Variety of Methods▪Scientific Knowledge is Based on Empirical Evidence▪Scientific Knowledge is Open to Revision in Light of New Evidence▪Scientific Models, Laws, Mechanisms, and Theories Explain Natural Phenomena ▪Science is a Way of Knowing▪Scientific Knowledge Assumes an Order and Consistency in Natural Systems▪Science is a Human Endeavor▪Science Addresses Questions About the Natural and Material WorldThe first four of these understandings are closely associated with practices and the second four with crosscutting concepts. The NOS Matrix presents specific content for K-2, 3-5, middle school and high school. Appropriate learning outcomes for the nature of science are expressed in the performance expectations, and presented in either the foundations column for practices or crosscutting concepts of the DCI standard pages.Again, one should note that the inclusion of nature of science in NGSS does not constitute a fourth dimension of standards. Rather, the grade level representations of the eight understandings have been incorporated in the practices and crosscutting concepts, as seen in the performance expectations and represented in the foundation boxes.Implementing Instruction to Facilitate Understanding of the Nature of Science Now, the science teacher’s question: How do I put the elements of practices and crosscutting concepts together to help students understand the nature of science? Suppose students observe the moon’s movements in the sky, changes in seasons, phase changes in water, or life cycles of organisms. One can have them observe patterns and propose explanations of cause-effect. Then, the students can develop a model of the system based on their proposed explanation. Next, they design an investigation to test the model. In designing the investigation, they have to gather data and analyze data. Next, they construct an explanation using an evidence-based argument. These experiences allow students to use their knowledge of the practices and crosscutting concepts to understand the nature of science. This is possible when students have instruction that emphasizes why explanations are based on evidence, that the phenomena they observe are consistent with the way the entire universe continues to operate, and that we can use multiple ways to investigate these phenomena.The Framework emphasizes that students must have the opportunity to stand back and reflect on how the practices contribute to the accumulation of scientific knowledge. This means, for example, that when students carry out an investigation, develop models, articulate questions, or engage in arguments, they should have opportunities to think about what they have done and why. They should be given opportunities to compare their own approaches to those of other students or professional scientists. Through this kind of reflection they can come to understand the importance of each practice and develop a nuanced appreciation of the nature of science.Using examples from the history of science is another method for presenting the nature of science. It is one thing to develop the practices and crosscutting concepts in the context of core disciplinary ideas; it is another aim to develop an understanding of the nature of science within those contexts. The use of case studies from the history of science provides contexts in which to develop students’ understanding of the nature of science. In the middle and high school grades, for example, case studies on the following topics might be used to broaden and deepen understanding about the nature of science.▪Copernican Resolution▪Newtonian Mechanics▪Lyell’s Study of Patterns of Rocks and Fossils▪Progression from Continental Drift to Plate Tectonics▪Lavoisier/Dalton and Atomic Structure▪Darwin Theory of Biological Evolution and the Modern Synthesis▪Pasteur and the Germ Theory of Disease▪James Watson and Francis Crick and the Molecular Model of GeneticsThese explanations could be supplemented with other cases from history. The point is to provide an instructional context that bridges tactics and strategies with practices and the nature of science, through understanding the role of systems, models, patterns, cause and effect, the analysis and interpretations of data, the importance of evidence with scientific arguments, and the construction of scientific explanations of the natural world. Through the use of historical and contemporary case studies, students can understand the nature of explanations in the larger context of scientific models, laws, mechanisms, and theories.In designing instruction, deliberate choices will need to be made about when it is sufficient to build students’ understanding of the scientific enterprise through reflection on their own investigations, and when it is necessary and productive to have students analyze historical case studies.ConclusionThis discussion addressed how to support the development of an understanding of the nature of science in the context of the Next Generation Science Standards. The approach centered on eight understandings for the nature of science and the intersection of those understandings with science and engineering practices, disciplinary core ideas, and crosscutting concepts. The nature of the scientific explanations is an idea central to standards-based science programs. Beginning with the practices, core ideas, and crosscutting concepts, science teachers can progress to the regularities of laws, the importance of evidence, and the formulation of theories in science. With the addition of historical examples, the nature of scientific explanations assumes a human face and is recognized as an ever-changing enterprise.ReferencesAmerican Association for the Advancement of Science (1993). Benchmarks for Science Literacy.New York: Oxford University Press.Conant, J. (1947). On Understanding Science: A Historical Approach. Cambridge, MA: Harvard University Press.Conant, J.B. (1951). Science and Common Sense. New Haven: Yale University Press. Duschl, R. (2012). The Second Dimension – Crosscutting Concepts: Understanding A Framework for K-12 Science Education.The Science Teacher, 79, 34-38.Duschl, R. (2008). Science Education in 3-part Harmony: Balancing Conceptual, Epistemic, and Social Learning Goals. In J. Green, A. Luke, & G. Kelly, Eds., Review of Research inEducation, 32 (pp. 268-291), Washington, DC: AERA.Duschl, R. (2000). Making the Nature of Science Explicit. In R. Millar, J. Leech & J. Osborne (Eds.), Improving Science Education: The Contribution of Research. Philadelphia, PA:Open University Press.Duschl, R. (1990). Restructuring Science Education: The Role of Theories and Their Importance. New York: Teachers College Press,Duschl, R., & Grandy, R. (Eds.). (2008). Teaching Scientific Inquiry: Recommendations for Research and Implementation. Rotterdam, Netherlands: Sense Publishers.Flick, L., & Lederman, M. (2004). Scientific Inquiry and Nature of Science. Boston, MA: Kluwer Academic Publishers.Klopfer, L., & Cooley, W. (1963). The History of Science Cases for High Schools in the Development of Student Understanding of Science and Scientists. Journal of Research in Science Teaching, 1(1), 33-47.Krajcik, J., & Merritt,J. (2012). Engaging Students in Scientific Practices: What Does Constructing and Revising Models Look Like in the Science Classroom? UnderstandingA Framework for K-12 Science Education. The Science Teacher, 79, 38-41. Lederman, N.G. (1992). Students’ and Teachers’ Conceptions of the Nature of Scien ce: A Review of the Research. Journal of Research in Science Teaching, 29(4), 331-359. Lederman, N., Abd-El-Khalick, F., Bell, R.L., & Schwartz, R.S. (2002). View of Nature of Science Questionnaire: Toward Valid and Meaningful Assessment of Learners’Conceptions of Nature of Science. Journal of Research in Science Teaching, 39(6): 497-521.Lederman, N., & Lederman, J. (2004). Revising Instruction to Teach Nature of Science: Modifying Activities to Enhance Students’ Understanding of Science. The ScienceTeacher, 71(9), 36-39.McComas, W. (Ed.). (1998). The Nature of Science in Science Education: Rationales and Strategies. Dordrecht: Kluwer Academic Publishers..National Research Council (2012). A Framework for K-12 Science Education: Practices, Crosscutting Concepts, and Core Ideas. Washington, DC: The National Academy Press.National Research Council (1996). National Science Education Standards. Washington, DC: The National Academy Press.Osborne, J.F., Ratcliffe, M., Collins, S., Millar, R., & Dusch l, R. (2003). What ‘Ideas about Science’ Should Be Taught in School Science? A Delphi Study of the ‘Expert’Community. Journal of Research in Science Teaching, 40(7), 692-720.Reiser, B., Berland, L, & Kenyon, L. (2012). Engaging Students in the Scientific Practices of Explanation and Argumentation: Understanding A Framework for K-12 ScienceEducation. The Science Teacher, 79(4), 8-13.Rutherford, F.J., & Ahlgren, A. (1989). Science for All Americans. New York: Oxford University Press, Inc.。

瑶药紫九牛中4种化学成分的提取分离、鉴定及含量测定

瑶药紫九牛中4种化学成分的提取分离、鉴定及含量测定

瑶药紫九牛中4种化学成分的提取分离、鉴定及含量测定Δ閤雪晴 1*,黄建猷 2, 3 #,黄周锋 2,高美美 1,黄宏妙 1,陆国寿 2(1.广西中医药大学药学院,南宁 530200;2.广西壮族自治区中医药研究院,南宁 530022;3.广西中药质量标准研究重点实验室,南宁 530022)中图分类号 R 917;R 284.1 文献标志码 A 文章编号 1001-0408(2024)05-0560-06DOI 10.6039/j.issn.1001-0408.2024.05.09摘要 目的 提取分离瑶药紫九牛中的4种化学成分并对其进行鉴定和含量测定。

方法 采用溶剂提取、萃取和硅胶柱色谱分离法、制备液相色谱技术对紫九牛中的化学成分进行分离、纯化,根据化合物的波谱数据对分离得到的4种化学成分进行结构鉴定。

采用高效液相色谱(HPLC )-一测多评(QAMS )法同时测定紫九牛中4种化学成分的含量,色谱条件为:以Echway Gowon TM C 18(250 mm ×4.6 mm ,5 μm )为色谱柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,检测波长为269 nm ,柱温为25 ℃;以大黄素为内参物,建立该成分与其他3种成分的相对校正因子,利用相对校正因子计算含量;同时采用外标法计算各成分的含量,并比较2种方法所得结果的差异。

结果 从紫九牛中分离得到的4种化学成分经鉴定分别为大黄素、欧鼠李苷A 、pleuropyrone A 、大黄素-8-O -β-D-葡萄糖苷。

HPLC-QAMS 法结果表明,pleuropyrone A 、大黄素-8-O -β-D-葡萄糖苷、欧鼠李苷A 的相对校正因子分别为1.147 2、0.874 7、0.644 4。

上述4种成分在各自检测范围内线性关系良好(r ≥0.999 6),精密度、稳定性、重复性试验的RSD 均小于2.00%,平均加样回收率为99.41%~100.46%(RSD ≤2.05%)。

基于GC-O-MS技术的植物肉风味特征及像真性分析

基于GC-O-MS技术的植物肉风味特征及像真性分析

李涵,李佳颖,刘娜,等. 基于GC-O-MS 技术的植物肉风味特征及像真性分析[J]. 食品工业科技,2023,44(24):247−258. doi:10.13386/j.issn1002-0306.2023020092LI Han, LI Jiaying, LIU Na, et al. Analysis of the Similarity of Flavoring Characteristic of Plant-based Meat Alternatives with Meat Products Based onGC-O-MS Technology[J]. Science and Technology of Food Industry, 2023, 44(24): 247−258. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020092· 分析检测 ·基于GC-O-MS 技术的植物肉风味特征及像真性分析李 涵1,李佳颖1,刘 娜2,董 婷2,孙媛霞3,邢竹青1,*(1.天津中医药大学公共卫生与健康科学学院,天津 301617;2.天津春发生物科技集团有限公司,天津 300300;3.国家合成生物技术创新中心,天津 300350)摘 要:为探究市售植物肉的风味特征及像真性,本实验采用固相微萃取法和气相色谱-嗅闻-质谱联用技术,对3类国产植物肉产品及对应种类动物肉产品的挥发性风味物质进行识别和差异分析。

在植物鸡肉、植物牛肉、植物猪肉中分别定性检出30,35和30种风味活性物质。

仪器测定结合相对气味活度值分析,明确构成三类植物肉的关键风味物质均以醛类物质为主,分别为庚醛(橡胶味、肉脂香)、(E )-2-辛烯醛(清香、肉脂香)、(E )-2-壬烯醛(肉脂香、咸味、酱香)、(E,E )-2,4-癸二烯醛(肉脂香)。

DERSIMELAGON 产品说明书

DERSIMELAGON 产品说明书

488 Scientific AbstractsSystemic sclerosis, myositis and related syndromes - aetiology, pathogenesis and animal modelsPOS0467 DERSIMELAGON, A NOVEL ORAL MELANOCORTIN1 RECEPTOR AGONIST, DEMONSTRATES DISEASE-MODIFYING EFFECTS IN PRECLINICAL MODELS OFSYSTEMIC SCLEROSISM. Kondo1, T. Suzuki1, Y. Kawano1, S. Kojima2, M. Miyashiro1, A. Matsumoto1, G. Kania3, P. Blyszczuk3, R. Ross4, P. Mulipa4, F. Del Galdo4, Y. Zhang5, J. H. W. Distler5. 1Mitsubishi T anabe Pharma Corporation, Research Unit/Immunology & Inflammation, Souyaku Innovative Research Division, Y okohama, Japan;2Mitsubishi T anabe Pharma Corporation, Discovery T echnology Laboratories, Souyaku Innovative Research Division, Y okohama, Japan;3University Hospital Zurich, University of Zurich, Center of Experimental Rheumatology, Department of Rheumatology, Schlieren, Switzerland;4University of Leeds, Leeds Instituteof Rheumatic and Musculoskeletal Medicine, Faculty of Medicine and Health, Leeds, United Kingdom;5Friedrich-Alexander-University Erlangen-Nürnberg (FAU) and University Hospital Erlangen, Department of Internal Medicine 3—Rheumatology and Immunology, Erlangen, GermanyBackground: Activation of melanocortin 1 receptor (MC1R) is known to have broad anti-inflammatory and anti-fibrotic effects. The bleomycin (BLM)-induced skin fibrosis murine model is well-established for systemic sclerosis (SSc). α-mel-anocyte-stimulating hormone, an endogenous ligand of MC1R, inhibits skin fibro-sis and MC1R knock-out enhances skin fibrosis in this model. These pieces of evidence suggest that MC1R agonism has potential in the treatment of SSc. Objectives: Dersimelagon phosphate (MT-7117) is an investigational small molecule that is an orally administered, selective agonist for MC1R. The purpose of this study is to investigate the potential of MT-7117 as a therapeutic agent for SSc by evaluat-ing its efficacy and mechanism of action in complementary preclinical models. The expression and distribution of MC1R in the skin of SSc patients was investigated. Methods: The effects of MT-7117 on skin fibrosis and lung inflammation were eval-uated in BLM-induced SSc murine models that were optimized for prophylactic and therapeutic evaluation. Microarray-based gene expression analysis and serum pro-tein profiling were performed to investigate the mechanism of action of MT-7117 in the BLM-induced SSc models. The effect of MT-7117 on TGF-β-induced activation of human dermal fibroblasts was evaluated in vitro. Immunohistochemical analyses of MC1R expression in skin samples from SSc patients were performed. Results: Prophylactic treatment with MT-7117 (≥0.3 mg/kg/day p.o.) significantly inhibited the increase in collagen content of the skin, the serum level of sur-factant protein D, and the weight of the lungs from BLM-induced skin fibrosis and lung inflammation model. Therapeutic treatment with MT-7117 (≥3 mg/kg/ day p.o.) significantly suppressed skin thickening and the numbers of myofi-broblasts in pre-established BLM-induced skin fibrosis model. Gene array anal-ysis using the BLM-induced SSc model demonstrated changes in numerous categories related to macrophages, monocytes, and neutrophils, followed by endothelial cell-related categories after treatment with MT-7117. In the analy-sis that focused on biological functions, categories of inflammatory response, activation of antigen-presenting cells, angiogenesis, atherosclerosis, vascu-logenesis, and vaso-occlusion were suppressed by MT-7117. In the analysis that focused on molecular signaling pathways, triggering receptor expressed on myeloid cells-1, IL-6, and oncostatin M involved in inflammation, and perox-isome proliferator-activated receptor that is related to fibrosis were all affected by MT-7117. Serum protein profiling using BLM-induced SSc model revealed that multiple SSc-related biomarkers including P-selectin, osteoprotegerin, cys-tatin C, growth and differentiation factor-15 and S100A9 were suppressed by MT-7117. MT-7117 inhibited the activation of human dermal fibroblasts by sup-pressing TGF-β-induced ACTA2 (encoding α-smooth muscle actin) mRNA ele-vation in vitro. Immunohistochemical analyses showed that MC1R positivity was observed in 40 of 50 diffuse cutaneous SSc patients. MC1R was expressed by monocytes/macrophages, neutrophils, blood vessels (endothelial cells), fibro-blasts, and epidermis (keratinocytes) in the skin of SSc patients. Conclusion: MT-7117 demonstrates disease-modifying effects in preclinical mod-els of SSc. Investigations of its mechanism of action and target expression anal-yses indicate that MT-7117 exerts its positive effects by affecting the pathologies of inflammation, vascular dysfunction, and fibrosis through inflammatory cells, endothelial cells, and fibroblasts. In view of its potent beneficial impact on all these three main pathologies of SSc, MT-7117 is a potential therapeutic agent for the treatment of clinically challenging SSc, which has diverse and difficult to treat symp-toms. A phase 2 clinical trial investigating the efficacy and tolerability of MT-7117 in patients with early, progressive diffuse cutaneous SSc is currently in progress. Disclosure of Interests: Masahiro Kondo Employee of: Mitsubishi Tanabe Pharma Corporation, Tsuyoshi Suzuki Employee of: Mitsubishi Tanabe Pharma Corporation, Yuko Kawano Employee of: Mitsubishi Tanabe Pharma Corpora-tion, Shinji Kojima Employee of: Mitsubishi Tanabe Pharma Corporation, Masa-hiko Miyashiro Employee of: Mitsubishi Tanabe Pharma Corporation, Atsuhiro Matsumoto Employee of: Mitsubishi Tanabe Pharma Corporation, Gabriela Kania: None declared, Przemyslaw Blyszczuk: None declared, rebecca ross:None declared, Panji Mulipa: None declared, Francesco Del Galdo Grant/ research support from: Prof. F. Del Galdo received fees and research supportfrom Abbvie, AstraZeneca, Boehringer-Ingelheim, Capella, Chemomab, Kymab, Janssen and Mitsubishi-Tanabe., Yun Zhang: None declared, Jörg H.W. DistlerGrant/research support from: Prof. J.H.W. Distler received consulting fees, lec-ture fees, and/or honoraria from Actelion, Active Biotech, Anamar, ARXX, aTyr,Bayer Pharma, Boehringer Ingelheim, Celgene, Galapagos, GSK, Inventiva, JB Therapeutics, Medac, Pfizer, Sanofi-Aventis, RedX, RuiYi and UCB. J. H. W.Distler is stock owner of 4D Science and Scientific head of FibroCure.DOI: 10.1136/annrheumdis-2022-eular.29POS0468 EXTRACELLULAR VESICLES FROM SERUM OFMYOSITIS PATIENTS AS CIRCULATING BIOMARKERSAND DISEASE MEDIATORSS. Kivity1,2, H. Kravitz3, C. Cohen3, D. Margoulis3, M. Amar3, G. Kazimirsky3,D. Ozeri4, A. Dori5, C. Brodie3. 1Meir Medical Center, Rheumatology Unit, KefarSava, Israel;2T el Aviv University, Sackler faculty of Medicine, T el Aviv-Y afo, Israel;3Bar-Ilan University, The Mina and Everard Goodman Faculty of Life Sciences,Ramat Gan, Israel;4T el-HaShomer The Sheba Medical Center, ZabludowiczCenter for Autoimmune Disease, Ramat Gan, Israel;5T el-HaShomer The ShebaMedical Center, Department of Neurology, T alpiot Medical Leadership Program,Sackler Faculty of Medicine, T el Aviv University, Ramat Gan, IsraelBackground: Inflammatory myopathies (IM) are a heterogeneous group of disor-ders characterized by autoimmune inflammatory destruction of skeletal muscles.It is many times associated with lung, skin and joint involvement. Identifying bio-markers that can differentiate IM from other muscle disorders may elucidate the pathophysiology of IM, guide novel therapies, monitor disease activity/responseto treatments and predict prognosis. Exosomes are membrane-bound nanove-sicles with diameters of 30-150 nm that contain multiple proteins, nucleic acid,lipids and other molecules in a tissue- and cell-specific manner. Exosomes are secreted by a large variety of cells, play major roles in cell-cell interactions, andhave recently emerged as circulating biomarkers in a variety of pathological con-ditions, including several autoimmune diseases.Objectives: To characterize exosomes from serum of IM patients, analyze pro-tein expression and study their potential mediators of disease pathologies.Methods: Serum was collected from patients suffering from IM(n=5) and from patients suffering from Becker (BMD) and Duchenne (DMD) muscular dystro-phies (n=6). Exosomes were isolated by Exoquick precipitation and analyzedfor size distribution and by nanoparticle tracking analysis (NTA) and by Westernblot for exosome markers. The effects of the isolated EVs on human satellitecell proliferation and differentiation and macrophage activation were examined. Results: Exosomes from IM patients decreased human satellite cell proliferation (51%, P<0.01) and inhibited their myogenic differentiation as indicated by lower fusionindex (24% inhibition, P<0.01) and expression of myosin heavy chain (72% inhibi-tion, P<0.001). Similar results were obtained also with exosomes derived from DMDand BMD patients; however, their inhibitory effect were more pronounced on MyoG expression. T reatment of macrophages with exosomes from IM patients significantly increased the expression of IL-10 (3-fold, P<0.001), compared to exosomes of healthy controls and DMD patients. Another significant difference was in the expression of sig-naling molecules: Thus, exosomes from BMD patients increased the phosphorylationof Erk and p38, whereas a smaller effect was induced by IM exosomes.Conclusion: Exosomes from IM patients decrease satellite cell proliferationand myogenic differentiation compared to healthy exosomes. In addition, these exosomes increased the expression of IL-10 in macrophages. These effects areunique to exosomes of IM patients compared to muscular dystrophies. These promising results suggest that serum exosomes should be further investigatedas a novel biomarker with potential therapeutic implications.Disclosure of Interests: Shaye Kivity Speakers bureau: BI, Abbvie, Lilly, Pfizer, Janssen, Neopharm, Grant/research support from: Sobi, Haya Kravitz: None declared, Coral Cohen: None declared, Darya Margoulis: None declared, MosheAmar: None declared, Gila Kazimirsky: None declared, David Ozeri Speakers bureau: Neopharm, Consultant of: Abbvie, Amir Dori Grant/research supportfrom: Biogen, Chaya Brodie Grant/research support from: Biogen.DOI: 10.1136/annrheumdis-2022-eular.63POS0469 ENDOTHELIAL TO MESENCHYMAL TRANSITIONAND SENESCENCE ARE PART OF THE FIBROTICPATHOGENESIS IN SYSTEMIC SCLEROSISY. H. Chiu1,2, J. Spierings1, J. M. Van Laar1, J. De Vries-Bouwstra3, M. VanDijk4, R. Goldschmeding4. 1University Medical Center Utrecht, Departmentof Rheumatology and Clinical Immunology, Utrecht, Netherlands;2T ri-ServiceGeneral Hospital, Division of Rheumatology/Immunology/Allergy, T aipei, T aiwan, Republic of China;3Leiden University Medical Center, The Department of on December 24, 2023 by guest. Protected by copyright./ Ann Rheum Dis: first published as 10.1136/annrheumdis-2022-eular.29 on 23 May 2022. Downloaded from。

备战高考英语名校模拟真题速递(江苏专用)专题06 阅读理解之说明文10篇(第六期)(含解析)

备战高考英语名校模拟真题速递(江苏专用)专题06 阅读理解之说明文10篇(第六期)(含解析)

备战高考英语名校模拟真题速递(江苏专用)第六期专题06 阅读理解之说明文10篇(2024·江苏南通·模拟预测)Mark Temple, a medical molecular (分子的) biologist, used to spend a lot of time in his lab researching new drugs for cancer treatments. He would extract DNA from cells and then add a drug to see where it was binding (结合) along the chemical sequence(序列). Before he introduced the drug, he’d look at DNA combination on a screen to see what might work best for the experiment, but the visual readout of the sequences was often unimaginably large.So Temple wondered if there was an easier way to detect favorable patterns. I realized I wanted to hear the sequence,” says Temple, who is also a musician. He started his own system of assigning notes to the different elements of DNA — human DNA is made of four distinct bases, so it was easy to start off with four notes — and made a little tune out of his materials. This trick indeed helped him better spot patterns in the sequences, which allowed him to make better choices about which DNA combinations to use.Temple isn’t the first person to turn scientific data into sound. In the past 40 years, researchers have gone from exploring this trick as a fun way to spot patterns in their studies tousing it as a guide to discovery. And the scientific community has come to realize that there’s some long-term value in this type of work. Temple, who from that first experiment has created his own algorithmic software to turn data into sound, believes the resulting music can be used to improve research and science communication.So Temple decided to add layers of sound to make the sonification (可听化) into songs. He sees a clear difference between “sonification” and “musification”. Using sound to represent data is scientific, but very different from using creative input to make songs. The musical notes from DNA may be melodic to the human ear, but they don’t sound like a song you’d listen to on the radio. So when he tried to sonify the virus, he added layers of drums and guitar, and had some musician friends add their own music to turn the virus into a full-blown post-rock song.Temple sees this work as an effective communication tool that will help a general audience understand complex systems in biology. He has performed his songs in public at concert halls in Australia.1.What is Mark Temple’s purpose in turning DNA data into sound?A.To help him fight boredom.B.To develop his creative ability.C.To make his drug more powerful.D.To aid the process of his experiments.2.What can we learn about Temple’s system?A.Its effect remains to be seen.B.It failed to work as expected.C.It is too complicated to operate.D.It has produced satisfying results.3.Why did Temple try to make the virus sound like real music when sonifying it?A.To get rid of public fear of the virus.B.To show h1s talent in producing music.C.To facilitate people’s understanding of science.D.To remind people or the roe or Science in art creation.4.What does the text mainly talk about?A.Why scientists are turning molecules into music.B.How scientists help the public understand science.C.Why music can be the best way to present science.D.How music helps scientists conduct their research.(2024·江苏南通·模拟预测)Phonics, which involves sounding out words syllable (音节) by syllable, is the best way to teach children to read. But in many classrooms, this can be a dirty word. So much so that some teachers have had to take phonics teaching materials secretly into the classroom. Most American children are taught to read in a way that study after study has found to be wrong.The consequences of this are striking. Less than half of all American adults were efficient readers in 2017. American fourth graders rank 15th on the Progress in International Literacy Study, an international exam.America is stuck in a debate about teaching children to read that has been going on for decades. Some advocate teaching symbol sound relationships (the sound k can be spelled as c, k, ck, or ch) known as phonics Others support an immersive approach (using pictures of cat to learn the word cat), known as “whole language”. Most teachers today, almost three out of four according to a survey by EdWeek Research Centre in 2019, use a mix of the two methods called “balanced literacy”.“A little phonics is far from enough.” says Tenette Smith, executive director of elementary education and reding at Mississippi’s education department. “It has to be systematic and explicitly taught.”Mississippi, often behind in social policy, has set an example here. In a state once blamed for its low reading scores, the Mississippi state legislature passed new literacy standards in 2013.Since then Mississippi has seen remarkable gains., Its fourth graders have moved from 49th (out of 50 states) to 20th on the National assessment of Educational Progress, a nationwide exam.Mississippi’s success is attributed to application of reading methods supported by a body of research known as the science of reading. In 1997 experts from the Department of Education ended the “reading war” and summed up the evidence. They found that phonics, along with explicit instruction in phonemic (音位的) awareness,fluency and comprehension, worked best.Yet over two decades on, “balanced literacy” is still being taught in classrooms. But advances in statistics and brain imaging have disproved the whole-language method. To the teacher who is an efficient reader, literacy seem like a natural process that requires educated guessing, rather than the deliberate process emphasized by phonics. Teachers can imagine that they learned to read through osmosis(潜移默化) when they were children. Without proper training, they bring this to classrooms.5.What do we learn about phonics in many American classrooms?A.It is ill reputed.B.It is mostly misapplied.C.It is totally ignored.D.It is seemingly contradictory.6.What has America been witnessing?A.A burning passion for improving teaching methods.B.A lasting debate over how to teach children to read.C.An increasing concern with children’s inadequacy in literacy.D.A forceful advocacy of a combined method for teaching reading.7.What’s Tenette Smith’s attitude towards “balanced literacy”?A.Tolerant.B.Enthusiastic.C.Unclear.D.Disapproving.8.According to the author what contributed to Mississippi’s success?A.Focusing on the natural process rather than deliberate training.B.Obtaining support from other states to upgrade teaching methods.C.Adopting scientifically grounded approaches to teaching reading.D.Placing sufficient emphasis upon both fluency and comprehension.(2024·江苏泰州·一模)A satellite is an object in space that orbits around another. It has two kinds — natural satellites and artificial satellites. The moon is a natural satellite that moves around the earth while artificial satellites are those made by man.Despite their widespread impact on daily life, artificial satellites mainly depend on different complicated makeups. On the outside, they may look like a wheel, equipped with solar panels or sails. Inside, the satellites contain mission-specific scientific instruments, which include whatever tools the satellites need to perform their work. Among them, high-resolution cameras and communication electronics are typical ones. Besides, the part that carries the load and holds all the parts together is called the bus.Artificial satellites operate in a systematic way just like humans. Computers function as the satellite’s brain, which receive information, interpret it, and send messages back to the earth. Advanced digital cameras serve asthe satellite’s eyes. Sensors are other important parts that not only recognize light, heat, and gases, but also record changes in what is being observed. Radios on the satellite send information back to the earth. Solar panels provide electrical power for the computers and other equipment, as well as the power to move the satellite forward.Artificial satellites use gravity to stay in their orbits. Earth’s gravity pulls everything toward the center of the planet. To stay in the earth’s orbit, the speed of a satellite must adjust to the tiniest changes in the pull of gravity. The satellite’s speed works against earth’s gravity just enough so that it doesn’t go speeding into space or falling back to the earth.Rockets carry satellites to different types and heights of orbits, based on the tasks they need to perform. Satellites closer to the earth are in low-earth orbit, which can be 200-500 miles high. The closer to the earth, the stronger the gravity is. Therefore, these satellites must travel at about 17,000 miles per hour to keep from falling back to the earth, while higher-orbiting satellites can travel more slowly.9.What is Paragraph 2 of the text mainly about?A.The appearance of artificial satellites.B.The components of artificial satellites.C.The basic function of artificial satellites.D.The specific mission of artificial satellites.10.What is the role of computers in artificial satellites?A.Providing electrical power.B.Recording changes observed.C.Monitoring space environment.D.Processing information received.11.How do artificial satellites stay in their orbits?A.By relying on powerful rockets to get out of gravity.B.By orbiting at a fixed speed regardless of gravity’s pull.C.By changing speed constantly based on the pull of gravity.D.By resisting the pull of gravity with advanced technologies.12.Why do satellites in higher-earth orbit travel more slowly?A.They are more affected by earth’s gravity.B.They take advantage of rockets more effectively.C.They have weaker pull of gravity in higher orbits.D.They are equipped with more advanced instruments.(2024·江苏泰州·一模)The human body possesses an efficient defense system to battle with flu viruses. The immune system protects against the attack of harmful microbes (微生物) by producing chemicals called antibodies, which are programmed to destroy a specific type of microbe. They travel in the blood and search the body for invaders (入侵者). When they find an invasive microbe, antibodies attack and destroy any cell thatcontains the virus. However, flu viruses can be a terrible enemy. Even if your body successfully fights against the viruses, with their ability to evolve rapidly, your body may have no protection or immunity from the new ones.Your body produces white blood cells to protect you against infectious diseases. Your body can detect invading microbes in your bloodstream because they carry antigens in their proteins. White blood cells in your immune system, such as T cells, can sense antigens in the viruses in your cells. Once your body finds an antigen, it takes immediate action in many different ways. For example, T cells produce more antibodies, call in cells that eat microbes, and destroy cells that are infected with a virus.One of the best things about the immune system is that it will always remember a microbe it has fought before and know just how to fight it again in the future. Your body can learn to fight so well that your immune system can completely destroy a virus before you feel sick at all.However, even the most cautious people can become infected. Fortunately, medical scientists have developed vaccines (疫苗), which are weakened or dead flu viruses that enter a person’s body before the person gets sick. These viruses cause the body to produce antibodies to attack and destroy the strong viruses that may invade during flu season.13.Why does flu pose a threat to the immune system?A.Microbes contain large quantities of viruses.B.Antibodies are too weak to attack flu viruses.C.The body has few effective ways to tackle flu.D.It’s hard to keep pace with the evolution of viruses.14.What does the underlined word “antigens” refer to in Paragraph 2?A.The cell protecting your body from viruses.B.The matter serving as the indicator of viruses.C.The antibodies helping to fight against viruses.D.The substance destroying cells infected with viruses.15.How do vaccines defend the body against the flu viruses?A.They strengthen the body’s immune system.B.They battle against weakened or dead viruses.C.They help produce antibodies to wipe out viruses.D.They expose the body to viruses during flu season.16.Which of the following is a suitable title for the text?A.Antibodies Save Our Health.B.Vaccines Are Of Great Necessity.C.Infectious Flu Viruses Are Around.D.Human Body Fights Against Flu Viruses.(23-24高三下·江苏扬州·开学考试)A recent study, led by Professor Andrew Barron, Dr. HaDi MaBouDi, and Professor James Marshall, illustrates how evolution has fine-tuned honey bees to make quick judgments while minimizing danger.“Animal lives are full of decisions,” says Professor Barron. “A honey bee has a brain smaller than a sesame (芝麻) seed. And yet it can make decisions faster and more accurately than’ we can. A robot programmed to do a bee’s job would need the backup of a supercomputer.”Bees need to work quickly and efficiently. They need to make decisions. Which flower will have a sweet liquid? While they’re flying, they face threats from the air. While landing, they’re vulnerable to potential hunter, some of which pretend to look like flowers.Researchers trained 20 bees to associate each of the five different colored “flower disks” with their visit history of reward and punishment. Blue flowers always had sugar juice. Green flowers always had a type of liquid with a bitter taste for bees. Other colors sometimes had glucose (葡萄糖). “Then we introduced each bee to a ‘garden’ with artificial ‘flowers’. We filmed each bee and timed their decision-making process,” says Dr. MaBouDi. “If the bees were confident that a flower would have food, they quickly decided to land on it, taking an average of 0.6 seconds. If they were confident that a flower wouldn’t have food, they made a decision just as quickly. If unsure, they took on average 1.4 seconds, and the time reflected the probability that a flower had food.”The team then built a computer model mirroring the bees’ decision-making process. They found the structure of the model looked very similar to the physical layout of a bee brain. “AI researchers can learn much from bees and other ‘simple’ animals. Millions of years of evolution has led to incredibly efficient brains with very low power requirements,” says Professor Marshall who co-founded a company that uses insect brain patterns to enable machines to move autonomously, like nature.17.Why does Professor Andrew Barron mention “a supercomputer”?A.To illustrate how a honey bee’s brain resemble each other.B.To explain how animals arrive at informed decisions fast.C.To demonstrate how a robot could finish a honey bee’s job.D.To emphasize how honey bees make decisions remarkably.18.Which of the following can best replace “vulnerable to” underlined in paragraph 3?A.Easily harmed by.B.Highly sensitive to.C.Deeply critical to.D.Closely followed by.19.What influenced the speed of trained bees in making decisions?A.Their judgments about reward and punishment.B.Their preference for the colors of flower disks.C.Their confirmation of food’s presence and absence.D.Their ability to tell real flowers from artificial ones.20.What message does Professor James Marshall want to give us?A.The power of bee brains is underestimated.B.Biology can inspire future AI.C.Autonomous machines are changing nature.D.AI should be far more efficient.(23-24高三下·江苏扬州·开学考试)Are you frequently overwhelmed by the feeling that life is leaving you behind, particularly when you look through social media sites and see all the exciting things your friends are up to? If so, you are not alone.FOMO, or Fear of Missing Out, refers to the perception that other people’s lives are superior to our own, whether this concerns socializing, accomplishing professional goals or generally having a more deeply fulfilling life. It shows itself as a deep sense of envy, and constant exposure to it can have a weakening effect on our self-respect. The feeling that we are always being left out of fundamentally important events, or that our lives are not living up to the image pictured by others, can have long-term damaging psychological consequences.While feelings of envy and inadequacy seem to be naturally human, social media seems to have added fuel to the fire in several ways. The reason why social media has such a triggering effect is tied to the appeal of social media in the first place: these are platforms which allow us to share only the most glowing presentations of our accomplishments, while leaving out the boring aspects of life. While this kind of misrepresentation could be characterized as dishonest, it is what the polished atmosphere of social media seems to demand.So how do we avoid falling into the trap of our own insecurities? Firstly, consider your own social media posts. Have you ever chosen photos or quotes which lead others to the rosiest conclusions about your life? Well, so have others and what they’ve left hidden is the fact that loneliness and boredom are unavoidably a part of everyone’s day-to-day life, and you are not the only one feeling left out. Secondly, learn to appreciate the positives. You may not be a regular at exciting parties or a climber of dizzying peaks, but you have your health, a place to live, and real friends who appreciate your presence in their lives. Last of all, learn to shake things off. We are all bombarded daily with images of other people’s perfection, but really, what does it matter? They are probably no more real than the most ridiculous reality TV shows.21.What can frequently experiencing FOMO lead to?A.Harm to one’s feeling of self-value.B.A more satisfying and fulfilling social life.C.Damage to one’s work productivity.D.Less likelihood of professional success.22.What does the author suggest in the third paragraph?A.The primary reason for FOMO is deeply rooted in social media.B.Our own social media posts help us feel much more confident.C.People who don’t share posts on social media are more bored.D.Social media’s nature enhances envious feelings and self-doubt.23.Why does the author mention reality TV shows in the last paragraph?A.To emphasize how false what we see on social media can be.B.To indicate how complicated social media has turned to.C.To figure out how popular and useful social media has been.D.To point out how educational value reality TV shows reflect.24.Which is the best title for the text?A.Myths and misconceptions about FOMO B.FOMO: what it is and how to overcome itC.How FOMO is changing human relationships D.We’re now all in the power of “FOMO addiction”(23-24高三上·江苏泰州·阶段练习)While Huawei’s official website does not call Mate 60 Pro a 5G smartphone, the phone’s wideband capabilities are on par with other 5G smartphones, raising a related question: As a leader in 5G technology, has Huawei managed to develop a 5G smartphone on its own?The answer is not simple. Huawei, as a pioneer in global 5G communication equipment, has played a leading role in the commercialization of 5G technology, with its strong system design and fields such as baseband chips (基带芯片), baseband processors and 5G modems.However, basebands and modems are not the only aspects that define 5G wireless communication. The stability and high-quality signals of a 5G smartphone also depend on other critical components such as RF transceivers (射频收发器) and RF front ends and antennas (天线) . These components are largely dominated by four US high-tech giants—Qualcomm, Avago Technologies, Ansem and Qorvo—which account for a surprising global market share.Huawei has faced significant challenges in getting critical components because of the sanctions imposed by the United States which are primarily responsible for the inability of the Chinese company to launch 5G smartphones in the past three years. However, Mate 60 Pro, despite not being labeled a 5G device, exhibits mobile network speeds comparable to Apple’s latest 5G-enabled devices, offering a stable communication experience. This suggests Huawei has, over the past three years, overcome the 5G development and production limits due to the US sanctions by cooperating with domestic partners, and establishing an independent and controllable stable supply chain.Considering that Huawei has not explicitly marketed this device as a 5G smartphone, it is possible that it isyet to fully overcome some key core technological and componential shortcomings. For the time being, we can consider Huawei’s Mate 60 Pro as 4.99G. But when combined with the satellite communication capabilities of Mate 60 Pro, it is clear Huawei has been trying to find more advanced wireless communication solutions for smartphones and making significant progress in this attempt. This should be recognized as a remarkable endeavor, even a breakthrough.25.What do the underlined words “on par with” mean in Paragraph 1?A.as poor as.B.as good as.C.worse than.D.better than.26.Why was it tough for Huawei to develop a 5G smartphone three years ago?A.Its system design and fields needed to be updated.B.It only focused on the commercialization of 5G technology.C.It was unwilling to cooperate with high-tech giants in America.D.It lacked critical components mainly controlled by US high-tech giants.27.What does Paragraph 4 centre on?A.The US sanctions.B.Critical components.C.Apple’s latest 5G-enabled devices.D.Progress in Mate 60 Pro.28.What is the text mainly about?A.Huawei faced with significant challengesB.Huawei’s Mate 60 Pro—a 5G smartphoneC.Huawei’s Mate 60 Pro—a remarkable breakthroughD.Huawei leading in global 5G communication equipment(23-24高三上·江苏无锡·期末)Blue-light-filtering glasses (滤蓝光眼镜) have become an increasingly popular solution for protecting our eyes from electronic screens’ near-inescapable glow — light that is commonly associated with eyestrain (眼疲劳). In recent years they’ve even become fashion statements that are recognized by celebrities and ranked in style guides. But a recent review paper shows such glasses might not be as effective as people think.The paper, published last week in Cochrane Database of Systematic Reviews, analyzed data from previous trials that studied how blue-light-filtering glasses affect vision tiredness and eye health. The study’s authors found that wearing blue-light-filtering glasses does not reduce the eyestrain people feel after using computers.“It’s an excellent review,” says Mark Rosenfield, a professor at the State University of New York College of Optometry, who was not involved in the study. “The conclusions are no surprise at all. There have been a number of studies that have found exactly the same thing, that there’s just no evidence that blue-blocking glasses have anyeffect on eyestrain.” He adds that the new review reinforces the fact that there is virtually no evidence that blue-blocking glasses affect eyestrain despite them being specifically marketed for that purpose. As for using blue-light-filtering eyeglasses for eye health, for now, Rosenfield says, “there’s nothing to support people buying them”.The strain we may feel while staring at our phone or computer screen too long is likely to be caused by multiple factors, such as bad habits or underlying conditions, an associate professor of vision science at the University of Melbourne, Downie says. She argues that how we interact with digital devices contributes more to eyestrain than screens’ blue light does. Changing the frequency and duration of screen usage and distancing one’s eyes from the screens might be more important in reducing discomfort, Downie says. She adds that people who experience eyestrain should see a doctor to assess whether they have an underlying health issue such as far-sightedness or dry eye disease.29.What can we know about blue-light-filtering glasses from the text?A.They can improve eyesight.B.They may not reduce eyestrain.C.They can promote eye health.D.They can help to cure eye diseases.30.What can we infer from paragraph 2?A.A great many professors were involved in the study.B.Blue-blocking glasses on the market are harmful to eyes.C.The finding of the study comes as a surprise to the public.D.Data from previous trials help the study a lot.31.What does the underlined word “reinforces” mean in paragraph 3?A.Denies.B.Opposes.C.Strengthens.D.Evaluates.32.What should we do if we suffer from eyestrain according to Downie?A.Wear blue-light-filtering glasses.B.Have an examination in the hospital.C.Stop staring at the screen for ever.D.Focus on the frequency of phone usage.(2024·江苏连云港·一模)Not all birds sing, but several thousand species do. They sing to defend their territory and croon (柔声唱) to impress potential mates. “Why birds sing is relatively well-answered,” says Iris Adam, a behavioral neuroscientist. However, the big question for her was why birds sing so much.“As soon as you sing, you reveal yourself,” Adam says. “Like, where you are and where your territory is.” In a new study published in the journal Nature Communications, Adam and her co-workers offer a new explanation for why birds take that risk. They may have to sing a lot every day to give their vocal (发声的) muscles the regular exercise they need to produce top-quality songs. To figure out whether the muscles that produce birdsongsrequire daily exercise, Adam designed an experiment on zebra finches-the little Australian songbirds.She prevented them from singing for a week by keeping them in the dark cage almost around the clock. Light is what galvanizes the birds to sing, so she had to work to keep them from warbling (鸣叫). “The first two or three days, it’s quite easy,” she says. “But the longer the experiment goes, the more they are like, ‘I need to sing.’” At that point, she’d tap the cage and tell them to stop singing.After a week, the birds’ singing muscles lost half their strength. But Adam wondered whether that impacted the quality of songs. When she played a male’s song before and after the seven days of darkness, she couldn’t hear a difference. But when Adam played it to a group of female birds, six out of nine preferred the song that came from a male who’d been using his singing muscles daily.Adam’s conclusion shows that “songbirds need to exercise their vocal muscles to produce top-performance songs. If they don’t sing, they lose performance, and their songs get less attractive to females.” This may help explain songbirds’ continuous singing.It’s a good rule to live by, whether you’re a bird or a human-practice makes perfect, at least when it comes to singing one’s heart out.33.According to Iris Adam, birds sing so much to ______.A.warn other birds of risks B.produce more songsC.perform perfectly in singing D.defend their territory34.What does the underlined word “galvanizes” in Paragraph 3 mean?A.Prepares.B.Stimulates.C.Forbids.D.Frightens.35.What do we know about the caged birds in the experiment?A.They lost the ability to sing.B.They strengthened their muscles.C.Their songs showed no difference.D.Their songs became less appealing.36.What may Iris Adam agree with?A.The songbirds live on music.B.The songbirds are born singers.C.Daily exercise keeps birds healthy.D.Practice makes birds perfect singers.(23-24高三上·江苏扬州·期末)Sometimes called “Earth’s twin,” Venus is similar to our world in size and composition. The two rocky planets are also roughly the same distance from the sun, and both have an atmosphere. While Venus’s cold and unpleasant landscape does make it seem far less like Earth, scientists recently detected another striking similarity between the two, the presence of active volcanoes.When NASA’s Magellan mission mapped much of the planet with radar in the 1990sit revealed an。

对细胞实验的理解 英语作文

对细胞实验的理解 英语作文

对细胞实验的理解英语作文Comprehending Cellular ExperimentationCellular experimentation is a fundamental aspect of scientific research, providing invaluable insights into the intricate workways of living organisms. Through the systematic investigation of cellular structures, functions, and interactions, researchers are able to unravel the mysteries of life at the most fundamental level. This essay aims to delve into the significance and complexities of cellular experimentation, exploring its methodologies, applications, and the crucial role it plays in advancing our understanding of the biological world.At the heart of cellular experimentation lies the desire to understand the building blocks of life – the cells. Cells are the basic units of all living organisms, responsible for carrying out the essential processes that sustain life. From the simplest unicellular organisms to the most complex multicellular systems, cells are the fundamental units that drive the biological machinery. Cellular experimentation, therefore, serves as a powerful tool to elucidate the inner workings of these microscopic entities, revealing the mechanisms that govern their structure, metabolism, signaling, and ultimately, their role in thelarger context of organismal function.One of the primary goals of cellular experimentation is to study the various components and organelles that make up a cell. Through techniques such as microscopy, biochemical analysis, and genetic manipulation, researchers can delve into the intricate details of cellular architecture, examining the functions and interactions of the nucleus, mitochondria, ribosomes, and other specialized structures. This knowledge not only enhances our understanding of cellular biology but also provides insights into the ways in which cells respond to environmental cues, undergo division and differentiation, and maintain homeostasis.Another crucial aspect of cellular experimentation is the investigation of cellular processes, including metabolism, signaling, and gene expression. By monitoring the dynamic changes in cellular activities, researchers can uncover the complex regulatory mechanisms that govern these fundamental processes. This information is invaluable in understanding the underlying causes of various diseases, as well as developing targeted therapies and interventions to address them.One of the most exciting frontiers in cellular experimentation is the field of stem cell research. Stem cells, with their unique ability to differentiate into a wide range of cell types, offer immense potential for regenerative medicine, tissue engineering, and the study ofdevelopmental biology. Through cellular experimentation, scientists are able to manipulate and direct the differentiation of stem cells, paving the way for groundbreaking advancements in the treatment of degenerative diseases, organ repair, and the development of personalized therapies.Moreover, cellular experimentation plays a crucial role in the development of new drugs and therapeutic strategies. By testing the effects of various compounds on cellular function, researchers can identify potential drug targets, assess the efficacy and safety of new treatments, and optimize drug delivery systems. This process is essential in the pharmaceutical industry, where cellular experiments serve as a critical step in the drug discovery and development pipeline.The methodologies employed in cellular experimentation are diverse and constantly evolving. From traditional techniques like cell culture and microscopy to more advanced approaches such as high-throughput screening, single-cell analysis, and genome editing, researchers have an expansive toolbox at their disposal. The integration of these cutting-edge technologies, coupled with computational and bioinformatic capabilities, has revolutionized the field of cellular experimentation, enabling researchers to generate and analyze vast amounts of data, uncover intricate cellular networks, and make unprecedented discoveries.However, the complexity of cellular systems also presents unique challenges in experimental design and data interpretation. Factors such as cellular heterogeneity, environmental influences, and the inherent variability of biological systems can complicate the interpretation of experimental results. Addressing these challenges requires a multidisciplinary approach, drawing on expertise from fields like systems biology, computational modeling, and advanced statistical analysis.Despite these challenges, the importance of cellular experimentation cannot be overstated. The insights gained from these investigations have far-reaching implications, informing our understanding of fundamental biological processes, disease mechanisms, and the development of innovative therapies. Moreover, cellular experimentation serves as a foundation for the broader field of life sciences, providing the necessary knowledge and tools to tackle complex problems in areas such as ecology, evolutionary biology, and biotechnology.In conclusion, cellular experimentation is a cornerstone of scientific research, offering a window into the intricate world of life at the most fundamental level. Through the systematic investigation of cellular structures, functions, and interactions, researchers are able to unravel the mysteries of living organisms, paving the way forgroundbreaking advancements in fields as diverse as medicine, genetics, and environmental science. As our understanding of cellular biology continues to evolve, the potential for cellular experimentation to drive scientific progress and improve the human condition remains immense and inspiring.。

最新理论试题及答案英语

最新理论试题及答案英语

最新理论试题及答案英语一、选择题(每题1分,共10分)1. The word "phenomenon" is most closely related to which of the following concepts?A. EventB. FactC. TheoryD. Hypothesis答案:C2. In the context of scientific research, what does the term "hypothesis" refer to?A. A proven factB. A testable statementC. A final conclusionD. An unverifiable assumption答案:B3. Which of the following is NOT a characteristic of scientific theories?A. They are based on empirical evidence.B. They are subject to change.C. They are always universally applicable.D. They are supported by a body of evidence.答案:C4. The scientific method typically involves which of the following steps?A. Observation, hypothesis, experimentation, conclusionB. Hypothesis, observation, conclusion, experimentationC. Experimentation, hypothesis, observation, conclusionD. Conclusion, hypothesis, observation, experimentation答案:A5. What is the role of experimentation in the scientific process?A. To confirm a hypothesisB. To disprove a hypothesisC. To provide evidence for or against a hypothesisD. To replace the need for a hypothesis答案:C6. The term "paradigm shift" in the philosophy of science refers to:A. A minor change in scientific theoryB. A significant change in the dominant scientific viewC. The process of scientific discoveryD. The end of scientific inquiry答案:B7. Which of the following is an example of inductive reasoning?A. Observing a pattern and making a general ruleB. Drawing a specific conclusion from a general ruleC. Making a prediction based on a hypothesisD. Testing a hypothesis through experimentation答案:A8. Deductive reasoning is characterized by:A. Starting with a specific observation and drawing a general conclusionB. Starting with a general rule and applying it to a specific caseC. Making assumptions without evidenceD. Relying on intuition rather than logic答案:B9. In scientific research, what is the purpose of a control group?A. To provide a baseline for comparisonB. To test an alternative hypothesisC. To increase the number of participantsD. To confirm the results of previous studies答案:A10. The principle of falsifiability, introduced by Karl Popper, suggests that:A. Scientific theories must be proven trueB. Scientific theories must be able to withstand attempts at being disprovenC. Scientific theories are never wrongD. Scientific theories are always based on personal beliefs答案:B二、填空题(每题1分,共5分)1. The scientific method is a systematic approach to__________ knowledge through observation, experimentation, and __________.答案:gaining; logical reasoning2. A scientific law is a statement that describes a__________ pattern observed in nature, while a scientific theory explains the __________ behind these patterns.答案:recurring; underlying principles3. The process of peer review in scientific publishing is important because it helps to ensure the __________ and__________ of research findings.答案:validity; reliability4. In the context of scientific inquiry, an __________ is a tentative explanation for an aspect of the natural world that is based on a limited range of __________.答案:hypothesis; observations5. The term "empirical" refers to knowledge that is based on __________ and observation, rather than on theory or__________.答案:experimentation; speculation三、简答题(每题5分,共10分)1. Explain the difference between a scientific theory and a scientific law.答案:A scientific theory is a well-substantiated explanation of some aspect of the natural world, based on a body of facts that have been repeatedly confirmed through observation and experimentation. It is a broad framework that can encompass multiple laws and observations. A scientific law, on the other hand, is a concise verbal or mathematical statement that describes a general pattern observed in nature. Laws summarize specific phenomena, while theories explain the broader principles behind those phenomena.2. What is the significance of the falsifiability criterionin the philosophy of science?答案:The falsifiability criterion, proposed byphilosopher of science Karl Popper, is significant because it provides a way to distinguish between scientific and non-scientific theories. For a theory to be considered scientific, it must be testable and potentially refutable by empirical evidence. This criterion ensures that scientific theories are open。

基于pyrF的乳酸乳球菌食品级表达载体的构建

基于pyrF的乳酸乳球菌食品级表达载体的构建

王帅,邓木兰,梁志成,等. 基于pyrF 的乳酸乳球菌食品级表达载体的构建[J]. 食品工业科技,2024,45(9):124−130. doi:10.13386/j.issn1002-0306.2023060149WANG Shuai, DENG Mulan, LIANG Zhicheng, et al. Construction of a Food-grade Expression Vector Based on pyrF Gene in Lactococcus lactis [J]. Science and Technology of Food Industry, 2024, 45(9): 124−130. (in Chinese with English abstract). doi:10.13386/j.issn1002-0306.2023060149· 生物工程 ·基于pyrF 的乳酸乳球菌食品级表达载体的构建王 帅1,邓木兰1, +,梁志成2,周泓宇1,何少媚1,张 智3,穆云萍1,李芳红1, *,赵子建1,*(1.广东工业大学生物医药研究院,广东广州 510006;2.华南理工大学医学院,广东广州 510006;3.深圳大学生命与海洋科学学院,广东深圳 518060)摘 要:基于pyrF 筛选标记和来源于乳酸乳球菌(Lactococcus lactis ,L. lactis )的基因组DNA 为表达元件,构建L. lactis 食品级表达载体,用于食品和药用多肽的表达和生产。

首先,利用NZ3900 pyrF 基因列构建同源重组突变盒,构建NZ3900 ΔpyrF 突变株;然后,分别以来源于L. lactis 的repA 和repC 基因为复制元件、pyrF 基因为筛选标记、P 32和P 8为启动子、以及T usp 45和T pepN 为终止子,构建食品级表达质粒pLD ;最后以绿色荧光蛋白ZsGreen 为报告基因,验证ZsGreen 在NZ3900 ΔpyrF 突变株的表达及pLD-ZsG 的遗传稳定性。

DMF模板

DMF模板

MODULE 3: QUALITY3.1 TABLE OF CONTENTS OF MODULE 3MODULE 3: QUALITY -----------------------------------------------------------------------------1 3.1 TABLE OF CONTENTS OF MODULE ------------------------------------------------------1 3.2BODY OF DATA----------------------------------------------------------------------------------3 3.2.S DRUG SUBSTANCE---------------------------------------------------------------------------3 3.2.S.1 General Information---------------------------------------------------------------------------3一般信息3.2.S.1.1 Nomenclature --------------------------------------------------------------------------------3命名3.2.S.1.2 Structure--------------------------------------------------------------------------------------4结构3.2.S.1.3 General Properties--------------------------------------------------------------------------5一般性质3.2.S.2 Manufacture------------------------------------------------------------------------------------6生产3.2.S.2.1 Manufacturer---------------------------------------------------------------------------------6生产商3.2.S.2.2 Description of Manufacturing Process and Process Controls------------------------16生产过程描述和过程控制描述3.2.S.2.3 Control of Materials------------------------------------------------------------------------27物料控制3.2.S.2.4 Controls of Critical Steps and Intermediates-------------------------------------------38关键步骤控制和关键中间体控制3.2.S.2.5 Process Validation and Evaluation-------------------------------------------------------51工艺验证和评估3.2.S.3 Characterization-------------------------------------------------------------------------------61定性3.2.S.3.1 Elucidation of Structure and Other Characteristics-----------------------------------61结构和其他特性阐述3.2.S.3.2 Impurities------------------------------------------------------------------------------------63杂质3.2.S.4 Control of Drug Substance------------------------------------------------------------------73原料药控制3.2.S.4.1 Specification and Routine Test Method -----------------------------------------------73质量标准和常规检测方法3.2.S.4.2 Analytical Procedures--------------------------------------------------------------------75检测操作规程3.2.S.4.3 Validation of Analytical Procedures----------------------------------------------------78检测操作规程验证3.2.S.4.4 Batch Analyses ---------------------------------------------------------------------------102批分析3.2.S.4.5 Justification of Specification------------------------------------------------------------103质量标准的论证3.2.S.5 Reference Standards or Materials--------------------------------------------------------104参照标准品或参照物质3.2.S.5.1 Classification and control---------------------------------------------------------------104类别和控制3.5.S.5.2 Standard Materials List------------------------------------------------------------------105标准品一览表3.2.S.6 Container Closure System-----------------------------------------------------------------106包装密封系统3.2.S.6.1 Packaging Materials Specification-----------------------------------------------------106包装材料质量标准3.2.S.6.2 Packaging Type---------------------------------------------------------------------------109包装类型3.2.S.7 Stability--------------------------------------------------------------------------------------110稳定性3.2.S.7.1 Stability Summary------------------------------------------------------------------------110稳定性总结3.2.S.7.2 Post-approval Stability Protocol--------------------------------------------------------110批准后的稳定性方案3.2.S.7.3 Stability Data------------------------------------------------------------------------------113稳定性数据3.2.A APPENDICES---------------------------------------------------------------------------------119附录3.2.A.1 Facilities and Equipment-------------------------------------------------------------------119设施和设备3.2A.2 Material Safe Data Sheet-------------------------------------------------------------------122原料安全数据表3.3 LITERATURE REFERENCES----------------------------------------------------------------126文献参考3.2 BODY OF DATA3.2.S DRUG SUBSTANCE (3.2.S.1 General Information3.2.S.1一般信息3.2.S.1.1 Nomenclature3.2.S.1.1 命名♦United States Adopted Name美国名称♦Pharmacopoeia Name of Product (Compendial Name)药典名称Ph.Eur.4th EditionBP2002USP26♦Active Component Chemical Name of Product (CAS,IUPAC)产品活性成份的化学名(CAS,IUPAC的命名)♦Company Name or laboratory codes公司名称或实验室代号♦Other nonproprietary names(e.g., International Nonproprietary Name(INN), British Approved Name(BAN), Japanese Accepted Name(JAN)其他非专用名称(INN名称,英国名称,日本名称)♦Chemical Abstracts Service(CAS) Registry Number化学文摘号3.2.S.1.2 Structure3.2.S.1.2 结构♦Structural Formula结构式♦Molecular Formula分子式♦Molecular Mass分子量3.2.S.1.3 General Properties3.2.S.1.3一般性质♦Physical —chemical properties理化性质A general description ( appearance, color, physical state) 综述(外观、颜色、物理状态)Melting or boiling points 熔点或沸点Solubility profile(aqueous and nonaqueous)溶解度(水溶液或非水溶液)UV maximum absorption最大紫外吸收Particle size颗粒度Density密度Tap density堆密度,Bulk density松密度Solution PH 溶液PH3.2.S.2 Manufacture3.2.S.2 生产3.2.S.2.1 Manufacturer3.2.S.2.1生产商♦Manufacturer Name and Address生产商名称和地址♦Production Site Address生产场地地址♦Name, telephone number, fax number and e-mail address of a contact person 联系人姓名、电话号码、传真号码、E-mail 地址♦Manufacturing responsibility生产商职责♦Commitment承诺♦Organizational Chart and Key Person组织机构图及关键人员♦Buildings and Facility建筑和设施A. Buildings OverviewA. 建筑综述说明厂房占地面积,建筑物数量及建筑面积。

高一科学探索英语阅读理解25题

高一科学探索英语阅读理解25题

高一科学探索英语阅读理解25题1<背景文章>The Big Bang Theory is one of the most important scientific theories in modern cosmology. It attempts to explain the origin and evolution of the universe. According to the Big Bang theory, the universe began as an extremely hot and dense singularity. Then, a tremendous explosion occurred, releasing an enormous amount of energy and matter. This event marked the beginning of time and space.In the early moments after the Big Bang, the universe was filled with a hot, dense plasma of subatomic particles. As the universe expanded and cooled, these particles began to combine and form atoms. The first atoms to form were hydrogen and helium. Over time, gravity caused these atoms to clump together to form stars and galaxies.The discovery of the cosmic microwave background radiation in 1964 provided strong evidence for the Big Bang theory. This radiation is thought to be the residual heat from the Big Bang and is uniformly distributed throughout the universe.The Big Bang theory has had a profound impact on modern science. It has helped us understand the origin and evolution of the universe, as well as the formation of stars and galaxies. It has also led to the development ofnew technologies, such as telescopes and satellites, that have allowed us to study the universe in greater detail.1. According to the Big Bang theory, the universe began as ___.A. a cold and empty spaceB. an extremely hot and dense singularityC. a collection of stars and galaxiesD. a large cloud of gas and dust答案:B。

高效液相色谱法同时测定饮用水中7种酰胺类农药

高效液相色谱法同时测定饮用水中7种酰胺类农药

高效液相色谱法同时测定饮用水中7种酰胺类农药尹明明,戴 维,杨 超,韩 倩,刘景龙(江苏省南京环境监测中心,江苏南京 210000)摘 要:目的:建立高效液相色谱法同时测定饮用水中7种酰胺类农药的方法。

方法:用二氯甲烷萃取水中的7种酰胺类农药,40 ℃水浴下氮吹至干,用乙腈复溶。

以水-乙腈为流动相,梯度洗脱,高效液相色谱法测定。

结果:7种酰胺类农药在0.5~100.0 mg·L-1线性关系良好,相关系数均大于0.999,检出限为0.002~0.008 μg·L-1,加标回收率在80.9%~97.3%,相对标准偏差在2.0%~6.9%。

结论:该方法的准确度高、精密度好,适用于饮用水中7种酰胺类农药的同时测定。

关键词:酰胺类农药;高效液相色谱法;饮用水Determination of 7 Amide Compounds in Drinking Water by High Performance Liquid ChromatographyYIN Mingming, DAI Wei, YANG Chao, HAN Qian, LIU Jinglong(Nanjing Environmental Monitoring Central of Jiangsu Province, Nanjing 210000, China) Abstract: Objective: To establish a HPLC method for simultaneous determination of 7 amide pesticides in drinking water. Method: Dichloromethane was used to extract 7 amide pesticides from drinking water. Nitrogen was blown to dry in water bath at 40 ℃, and then filtered by acetonitrile. Using water acetonitrile as mobile phase, gradient elution was performed and determined by high-performance liquid chromatography. Result: The linear relationship between 7 amide pesticides is good at 0.5~100.0 mg·L-1, and the correlation coefficients are all greater than 0.999. The detection limits were 0.002~0.008 μg·L-1. The recoveries were 80.9%~97.3% and the relative standard deviations were 2.0%~6.9%. Conclusion: The method has high accuracy and good precision, and is suitable for the determination of 7 amide pesticides in drinking water.Keywords: amide compounds; high performance liquid chromatography; drinking water酰胺类农药主要用于玉米、大豆、花生、棉花和甘蔗等禾本科杂草和小粒种子杂草等。

高中英语说明文单选题50题

高中英语说明文单选题50题

高中英语说明文单选题50题1. The doctor suggested that he should stay in bed for a few days to ______ his illness.A. get overB. go overC. turn overD. come over答案:A。

“get over”有“克服;恢复;熬过”的意思,在此表示恢复健康,符合语境。

“go over”是“复习;仔细检查”,“turn over”是“翻转;移交”,“come over”是“顺便来访;突然感到”,都不符合“从疾病中恢复”的意思。

2. She is very busy these days. She has a lot of work to ______.A. take upB. come upC. put upD. make up答案:A。

“take up”有“从事;占据”的意思,此处指有很多工作要做。

“come up”是“走近;发生;被提出”,“put up”是“张贴;搭建;举起”,“make up”是“组成;化妆;编造”,都不符合“有工作要做”的语境。

3. The old man was too weak to ______ the heavy box.A. carry outB. carry offC. carry onD. carry答案:D。

“carry”表示“搬运”,“carry out”是“执行;实行”,“carry off”是“夺去;抢走”,“carry on”是“继续;坚持”,这里指老人太虚弱搬不动重箱子,用“carry”最合适。

4. We should ______ our best to help those in need.A. tryB. doC. makeD. take答案:B。

“do one's best”是固定短语,表示“尽力”,A 选项“try”通常与“try one's best”搭配,C 选项“make”没有“make one's best”的用法,D 选项“take”也不符合,所以选B。

2024年高考英语试卷

2024年高考英语试卷

2024年高考英语真题试卷(新高考Ⅰ卷)第二部分一、阅读(共两节,满分50分)第一节(共15小题;每小题2.5分,满分37.5分)阅读下列短文,从每题所给的A、B、C、D四个选项中选出最佳选项。

HABITAT RESTORATIONTEAMHelp restore and protect Marin's natural areas from the Marin Headlands to Bolinas Ridge. We'll explore beautiful park sites while conducting invasive(侵入的)plant removal, winter planting, and seed collection. Habitat Restoration Team volunteers play a vital role in restoring sensitive resources and protecting endangered species across the ridges and valleys.GROUPSGroups of five or more require special arrangements and must be confirmed in advance. Please review the List of Available Projects and fill out the Group Project Request Form.AGE, SKILLS, WHAT TO BRINGV olunteers aged 10 and over are welcome. Read our Youth Policy Guidelines for youth under the age of 15.Bring your completed V olunteer Agreement Form. V olunteers under the age of18 must have the parent /guardian approval section signed.We'll be working rain or shine. Wear clothes that can get dirty. Bring layers for changing weather and a raincoat if necessary.Bring a personal water bottle, sunscreen, and lunch.No experience necessary. Training and tools will be provided. Fulfills(满足)community service requirements.UPCOMING EVENTS1.What is the aim of the Habitat Restoration Team?A.To discover mineral resources.B.To develop new wildlife parks.C.To protect the local ecosystemD.To conduct biological research.2.What is the lower age limit for joining the Habitat Restoration Team?A.5.B.10.C.15.D.18.3.What are the volunteers expected to do?A.Bring their own tools.B.Work even in bad weather.C.Wear a team uniform D.Do at least three projects."I am not crazy, "says Dr. William Farber, shortly after performing acupuncture (针灸) on a rabbit. "I am ahead of my time. "If he seems a little defensive, it might be because even some of his coworkers occasionally laugh at his unusual methods, But Farber is certain he'll have the last laugh. He's one of a small but growing number of American veterinarians(兽医)now practicing "holistic" medicine-combining traditional Western treatments with acupuncture, chiropractic(按摩疗法)and herbal medicine Farber, a graduate of Colorado State University, started out as a more conventional veterinarian. He became interested in alternative treatments 20 years ago when he suffered from terrible back pain. He tried muscle-relaxing drugs but found little relief. Then he tried acupuncture, an ancient Chinese practice, and was amazed that he improved after two or three treatments. What worked on a veterinarian seemed likely to work on his patients. So, after studying the techniques for a couple of years, he began offering them to pets Leigh Tindale's dog Charlie had a serious heart condition. After Charlie had a heart attack, Tindale says, she was prepared to put him to sleep, but Farber's treatments eased her dog's suffering so much that she was able to keep him alive for an additional five months And Priscilla Dewing reports that her horse, Nappy, "moves more easily and rides more comfortably" after a chiropractic adjustment.Farber is certain that the holistic approach will grow more popular with time, and if the past is any indication, he may be right: Since 1982, membership in the American Holistic Veterinary Medical Association has grown from 30 to over 700. "Sometimes it surprises me that it works so well, "he says. "I will do anything to help an animal. That's my job. "4.What do some of Farber's coworkers think of him?A.He's odd.B.He's strict C.He's brave.D.He's rude5.Why did Farber decide to try acupuncture on pets?A.He was trained in it at university.B.He was inspired by another veterinarian.C.He benefited from it as a patient.D.He wanted to save money for pet owners.6.What does paragraph 3 mainly talk about?A.Steps of a chiropractic treatment.B.The complexity of veterinarians' work.C.Examples of rare animal diseases.D.The effectiveness of holistic medicine.7.Why does the author mention the American Holistic Veterinary Medical Association?A.To prove Farber's point B.To emphasize its importance.C.To praise veterinarians.D.To advocate animal protection.Is comprehension the same whether a person reads a text onscreen or on paper? And are listening to and viewing content as effective as reading the written word when covering the same material? The answers to both questions are often "no. " The reasons relate to a variety of factors, including reduced concentration, an entertainment mindset(心态)and a tendency to multitask while consuming digital content.When reading texts of several hundred words or more, learning is generally more successful when it's on paper than onscreen. A large amount of research confirms this finding. The benefits of print reading particularly shine through when experimenters move from posing simple tasks-like identifying the main idea in a reading passage-to ones that require mental abstraction-such as drawing inferences from a text.The differences between print and digital reading results are partly related to paper's physical properties. With paper, there is a literal laying on of hands, along with the visual geography of distinct pages. People often link their memory of what they've read to how far into the book it was or where it was on the page.But equally important is the mental aspect. Reading researchers have proposed a theory called "shallowing hypothesis(假说). " According to this theory, people approach digital texts with a mindset suited to social media, which are often not so serious, and devote less mental effort than when they are reading print Audio(音频)and video can feel more engaging than text, and so university teachers increasingly tum to these technologies -say, assigning an online talk instead of an article by the same person. However, psychologists have demonstrated that when adults read news stories, they remember more of the content than if they listen to or view identical piecesDigital texts, audio and video all have educational roles, especially when providing resources not available in print. However, for maximizing leaning where mental focus and reflection are called for, educators shouldn't assume all media are the same, even when they contain identical words.8.What does the underlined phrase "shine through" in paragraph 2 mean?A.Seem unlikely to last.B.Seem hard to explain.C.Become ready to use.D.Become easy to notice.9.What does the shallowing hypothesis assume?A.Readers treat digital texts lightly.B.Digital texts are simpler to understand.C.People select digital texts randomly.D.Digital texts are suitable for social media.10.Why are audio and video increasingly used by university teachers?A.They can hold students' attentionB.They are more convenient to prepare.C.They help develop advanced skills.D.They are more informative than text.11.What does the author imply in the last paragraph?A.Students should apply multiple learning techniques.B.Teachers should produce their own teaching material.C.Print texts cannot be entirely replaced in education.D.Education outside the classroom cannot be ignored.In the race to document the species on Earth before they go extinct, researchers and citizen scientists have collected billions of records. Today, most records of biodiversity are often in the form of photos, videos, and other digital records. Though they are useful for detecting shifts in the number and variety of species in an area, a new Stanford study has found that this type of record is not perfect."With the rise of technology it is easy for people to make observations of different species with the aid of a mobile application, "said Barnabas Daru, who is lead author of the study and assistant professor of biology in the Stanford School of Humanities and Sciences. "These observations now outnumber the primary data that comes from physical specimens(标本), and since we are increasingly using observational data to investigate how species are responding to global change, I wanted to know: Are they usable?"Using a global dataset of 1. 9 billion records of plants, insects, birds, and animals, Daru and his team tested how well these data represent actual global biodiversity patterns."We were particularly interested in exploring the aspects of sampling that tend to bias(使有偏差)data, like the greater likelihood of a citizen scientist to take a picture of af lowering plant instead of the grass rightnext to it, "said Daru.Their study revealed that the large number of observation-only records did not lead to better global coverage. Moreover, these data are biased and favor certain regions, time periods, and species. This makes sense because the people who get observational biodiversity data on mobile devices are often citizen scientists recording their encounters with species in areas nearby. These data are also biased toward certain species with attractive or eye-catching features.What can we do with the imperfect datasets of biodiversity?"Quite a lot, "Daru explained." Biodiversity apps can use our study results to inform users of oversampled areas and lead them to places -and even species -that are not well-sampled. To improve the quality of observational data, biodiversity apps can also encourage users to have an expert confirm the identification of their uploaded image. "12.What do we know about the records of species collected now?A.They are becoming outdated.B.They are mostly in electronic formC.They are limited in numberD.They are used for public exhibition.13.What does Daru's study focus on?A.Threatened species.B.Physical specimens.C.Observational data D.Mobile applications14.What has led to the biases according to the study?A.Mistakes in data analysis.B.Poor quality of uploaded picturesC.Improper way of sampling.D.Unreliable data collection devices.15.What is Daru's suggestion for biodiversity apps?A.Review data from certain areas.B.Hire experts to check the records.C.Confirm the identity of the users.D.Give guidance to citizen scientists.二、第二节(共5小题;每小题2.5分,满分12.5分)(2024·新高考Ⅰ卷)阅读下面短文,从短文后的选项中选出可以填入空白处的最佳选项。

anhydrous for analysis emsure -回复

anhydrous for analysis emsure -回复

anhydrous for analysis emsure -回复Anhydrous for Analysis EMSURE: Understanding Its Importance and ApplicationsIntroduction:Anhydrous for Analysis EMSURE is a high-quality reagent widely used in various scientific disciplines and industries. It plays a crucial role in ensuring accurate and reliable analytical results. In this article, we will explore in detail the significance, properties, and applications of Anhydrous for Analysis EMSURE, thereby providing a comprehensive understanding of this essential reagent.1. What is Anhydrous for Analysis EMSURE?Anhydrous for Analysis EMSURE is a term used to describe a broad range of reagents that are completely free from water molecules. These reagents are produced using advanced techniques to remove any moisture content, ensuring maximum stability and purity. Anhydrous for Analysis EMSURE is typically available in ultra-pure forms, meeting the highest quality standards demanded by analytical laboratories.2. Importance of Anhydrous for Analysis EMSURE:2.1. Eliminating Water Interference:Water is a common impurity in many chemicals used in analytical processes. However, the presence of water can interfere with various reactions and measurements, leading to inaccurate results. Anhydrous for Analysis EMSURE eliminates this interference, allowing for precise and reliable analysis.2.2. Enhanced Stability:Water can initiate degradation processes in certain substances, affecting their stability over time. Anhydrous for Analysis EMSURE, being entirely free from water, exhibits superior stability and prolonged shelf life. This property is especially critical for long-term storage of reagents and standards.2.3. Prevention of Hydrate Formation:Certain compounds readily react with water, forming hydrates—a chemically combined form where water molecules are incorporated into the substance's crystal lattice. Anhydrous for Analysis EMSURE prevents hydrate formation, maintaining the integrity of thecompound and ensuring accurate analysis.3. Properties of Anhydrous for Analysis EMSURE:3.1. Low Water Content:Anhydrous for Analysis EMSURE reagents typically have an extremely low moisture content, often in the range of parts per million (ppm) or below. This ensures minimal water-related interference during analytical procedures.3.2. High Purity:To meet the stringent requirements of analytical applications, Anhydrous for Analysis EMSURE reagents are manufactured to possess high purity levels. They undergo rigorous quality control measures, including multiple purification steps, to eliminate impurities that could affect the accuracy of analytical results.3.3. Traceable Certification:Anhydrous for Analysis EMSURE reagents are accompanied by comprehensive certificates of analysis, detailing the quality, purity, and conformity of the product. These certificates provide traceability and help maintain consistency in analytical procedures.4. Applications of Anhydrous for Analysis EMSURE:4.1. Chemical Analysis:Anhydrous for Analysis EMSURE reagents are widely used in various chemical analyses, including titrations, spectrophotometry, chromatography, and atomic absorption spectroscopy. Their water-free nature ensures accurate measurements and consistent results.4.2. Pharmaceutical Industry:In the pharmaceutical industry, Anhydrous for Analysis EMSURE is invaluable for conducting quality control tests, formulation development, and stability studies. It helps ensure the purity and stability of drug substances and excipients, thus contributing to the production of safe and effective medications.4.3. Food and Beverage Industry:Anhydrous for Analysis EMSURE reagents find extensive utility in the food and beverage industry. They are employed for the analysis of food components, additives, and contaminants, ensuring compliance with regulatory standards and ensuring consumersafety.4.4. Environmental Analysis:In environmental analysis, Anhydrous for Analysis EMSURE reagents aid in monitoring pollution levels, assessing the quality of water and air, and investigating the impact of pollutants on the environment. The absence of water interference allows for precise measurements and reliable data.5. Conclusion:Anhydrous for Analysis EMSURE is an indispensable reagent that plays a vital role in ensuring accurate and reliable analysis across various scientific disciplines and industries. Its ability to eliminate water interference, enhance stability, and prevent hydrate formation makes it a preferred choice for a wide range of applications. By understanding the significance and properties of Anhydrous for Analysis EMSURE, researchers and analysts can confidently employ this high-quality reagent to obtain precise and consistent results.。

高考英语:重难点3“阅读理解之说明文”真题分析

高考英语:重难点3“阅读理解之说明文”真题分析

重难点03 阅读理解之说明文【命题趋势】最近五年,说明文的出现变化不大,一直很稳定,选材通常是各学科的前沿问题;高科技领域的科研成果;人们比较关心的社会问题;人文方面的经典。

由于阅读理解题的设置采用渐进式,即由简到难的方式,因此说明文是高考试卷中阅读理解题中相对比较难的,通常后置。

说明文的词汇和句式的运用较别的体裁的文章难度更大。

词汇运用灵活,同一词的不同词性的用法交替出现,未列入考纲的生词较多,通常达到了4-5%。

不过考生可以通过说明文的语言特点来帮助理解语篇,例如,下定义、解释、举例、同义词、反义词、上下义词以及标点符号(如破折号、冒号都有表示解释和说明)等。

【满分技巧】高考阅读理解中,说明文为主要体裁之一。

高考阅读理解题的设问主要围绕以下四方面:细节事实题、主旨大意题、推理判断题、猜测词义题。

其中,说明文主要以细节事实、主旨大意和猜测词义三方面问题为主。

一、词义猜测类题型阅读理解题中常要求学生猜测某些单词或短语的意思。

词义的猜测还是贯穿在文章的阅读理解之中。

解这类题目一般是通过上下文去理解或根据构词法去猜测。

判断一个单词的意思不但离不开句子,而且还需要把句子放在上下文中,根据上下文提供的线索加以猜测。

运用构词法,语境等推测关键词义,可以根据以下几种方法猜测:(一)内在逻辑关系根据内在逻辑关系推测词义是指运用语言知识分析和判断相关信息之间存在的逻辑关系,然后根据逻辑关系推断生词词义。

1.通过同义词和反义词的关系猜词2.根据因果关系猜测词义3.通过定义或释义关系来推测词义4.通过句法功能来推测词义5.通过描述猜词(二)外部相关因素外部相关因素是指篇章(句子或段落)以外的其他知识,有时仅靠分析篇章内在逻辑关系无法猜出词义。

这时,就需要运用生活经验和普通常识确定词义。

例如:The snakes lithered through the grass.根据有关蛇的生活习性的知识,我们可以推断出slither词义为"爬行"。

中国诺奖级别新科技—量子反常霍尔效应英语

中国诺奖级别新科技—量子反常霍尔效应英语

中国诺奖级别新科技—量子反常霍尔效应英语全文共6篇示例,供读者参考篇1The Magical World of Quantum PhysicsHave you ever heard of something called quantum physics? It's a fancy word that describes the weird and wonderful world of tiny, tiny particles called atoms and electrons. These particles are so small that they behave in ways that seem almost magical!One of the most important discoveries in quantum physics is something called the Quantum Anomalous Hall Effect. It's a mouthful, I know, but let me try to explain it to you in a way that's easy to understand.Imagine a road, but instead of cars driving on it, you have electrons zipping along. Now, normally, these electrons would bump into each other and get all mixed up, just like cars in a traffic jam. But with the Quantum Anomalous Hall Effect, something special happens.Picture a big, strong police officer standing in the middle of the road. This police officer has a magical power – he can makeall the electrons go in the same direction, without any bumping or mixing up! It's like he's directing traffic, but for tiny particles instead of cars.Now, you might be wondering, "Why is this so important?" Well, let me tell you! Having all the electrons moving in the same direction without any resistance means that we can send information and electricity much more efficiently. It's like having a super-smooth highway for the electrons to travel on, without any potholes or roadblocks.This discovery was made by a team of brilliant Chinese scientists, and it's so important that they might even win a Nobel Prize for it! The Nobel Prize is like the Olympic gold medal of science – it's the highest honor a scientist can receive.But the Quantum Anomalous Hall Effect isn't just about winning awards; it has the potential to change the world! With this technology, we could create faster and more powerful computers, better ways to store and transfer information, and even new types of energy篇2China's Super Cool New Science Discovery - The Quantum Anomalous Hall EffectHey there, kids! Have you ever heard of something called the "Quantum Anomalous Hall Effect"? It's a really cool andmind-boggling scientific discovery that scientists in China have recently made. Get ready to have your mind blown!Imagine a world where electricity flows without any resistance, like a river without any rocks or obstacles in its way. That's basically what the Quantum Anomalous Hall Effect is all about! It's a phenomenon where electrons (the tiny particles that carry electricity) can flow through a material without any resistance or energy loss. Isn't that amazing?Now, you might be wondering, "Why is this such a big deal?" Well, let me tell you! In our regular everyday world, when electricity flows through materials like wires or circuits, there's always some resistance. This resistance causes energy to be lost as heat, which is why your phone or computer gets warm when you use them for a long time.But with the Quantum Anomalous Hall Effect, the electrons can flow without any resistance at all! It's like they're gliding effortlessly through the material, without any obstacles or bumps in their way. This means that we could potentially have electronic devices and circuits that don't generate any heat or waste any energy. How cool is that?The scientists in China who discovered this effect were studying a special kind of material called a "topological insulator." These materials are like a secret passageway for electrons, allowing them to flow along the surface without any resistance, while preventing them from passing through the inside.Imagine a river flowing on top of a giant sheet of ice. The water can flow freely on the surface, but it can't pass through the solid ice underneath. That's kind of how these topological insulators work, except with electrons instead of water.The Quantum Anomalous Hall Effect happens when these topological insulators are exposed to a powerful magnetic field. This magnetic field creates a special condition where the electrons can flow along the surface without any resistance at all, even at room temperature!Now, you might be thinking, "That's all well and good, but what does this mean for me?" Well, this discovery could lead to some pretty amazing things! Imagine having computers and electronic devices that never overheat or waste energy. You could play video games or watch movies for hours and hours without your devices getting hot or draining their batteries.But that's not all! The Quantum Anomalous Hall Effect could also lead to new and improved ways of generating, storing, and transmitting energy. We could have more efficient solar panels, better batteries, and even a way to transmit electricity over long distances without any energy loss.Scientists all around the world are really excited about this discovery because it opens up a whole new world of possibilities for technology and innovation. Who knows what kind of cool gadgets and devices we might see in the future thanks to the Quantum Anomalous Hall Effect?So, there you have it, kids! The Quantum Anomalous Hall Effect is a super cool and groundbreaking scientific discovery that could change the way we think about electronics, energy, and technology. It's like something straight out of a science fiction movie, but it's real and happening right here in China!Who knows, maybe one day you'll grow up to be a scientist and help us unlock even more amazing secrets of the quantum world. Until then, keep learning, keep exploring, and keep being curious about the incredible wonders of science!篇3The Wonderful World of Quantum Physics: A Journey into the Quantum Anomalous Hall EffectHave you ever heard of something called quantum physics? It's a fascinating field that explores the strange and mysterious world of tiny particles called atoms and even smaller things called subatomic particles. Imagine a world where the rules we're used to in our everyday lives don't quite apply! That's the world of quantum physics, and it's full of mind-boggling discoveries and incredible phenomena.One of the most exciting and recent breakthroughs in quantum physics comes from a team of brilliant Chinese scientists. They've discovered something called the Quantum Anomalous Hall Effect, and it's like a magic trick that could change the way we think about technology!Let me start by telling you a bit about electricity. You know how when you turn on a light switch, the bulb lights up? That's because electricity is flowing through the wires and into the bulb. But did you know that electricity is actually made up of tiny particles called electrons? These electrons flow through materials like metals and give us the electricity we use every day.Now, imagine if we could control the flow of these electrons in a very precise way, like directing them to move in a specificdirection without any external forces like magnets or electric fields. That's exactly what the Quantum Anomalous Hall Effect allows us to do!You see, in most materials, electrons can move in any direction, like a group of kids running around a playground. But in materials that exhibit the Quantum Anomalous Hall Effect, the electrons are forced to move in a specific direction, like a group of kids all running in a straight line without any adults telling them where to go!This might not seem like a big deal, but it's actually a huge deal in the world of quantum physics and technology. By controlling the flow of electrons so precisely, we can create incredibly efficient electronic devices and even build powerful quantum computers that can solve problems much faster than regular computers.The Chinese scientists who discovered the Quantum Anomalous Hall Effect used a special material called a topological insulator. This material is like a magician's hat – it looks ordinary on the outside, but it has some really weird and wonderful properties on the inside.Inside a topological insulator, the electrons behave in a very strange way. They can move freely on the surface of the material, but they can't move through the inside. It's like having篇4The Coolest New Science from China: Quantum Anomalous Hall EffectHey kids! Have you ever heard of something called the Quantum Anomalous Hall Effect? It's one of the most amazing new scientific discoveries to come out of China. And get this - some scientists think it could lead to a Nobel Prize! How cool is that?I know, I know, the name sounds kind of weird and complicated. But trust me, once you understand what it is, you'll think it's just as awesome as I do. It's all about controlling the movement of tiny, tiny particles called electrons using quantum physics and powerful magnetic fields.What's Quantum Physics?Before we dive into the Anomalous Hall Effect itself, we need to talk about quantum physics for a second. Quantum physics is sort of like the secret rules that govern how the smallest things inthe universe behave - things too tiny for us to even see with our eyes!You know how sometimes grown-ups say things like "You can't be in two places at once"? Well, in the quantum world, particles actually can be in multiple places at the same time! They behave in ways that just seem totally bizarre and counterintuitive to us. That's quantum physics for you.And get this - not only can quantum particles be in multiple places at once, but they also spin around like tops! Electrons, which are one type of quantum particle, have this crazy quantum spin that makes them act sort of like tiny magnets. Mind-blowing, right?The Weirder Than Weird Hall EffectOkay, so now that we've covered some quantum basics, we can talk about the Hall Effect. The regular old Hall Effect was discovered way back in 1879 by this dude named Edwin Hall (hence the name).Here's how it works: if you take a metal and apply a magnetic field to it while also running an electrical current through it, the magnetic field will actually deflect the flow of electrons in the metal to one side. Weird, huh?Scientists use the Hall Effect in all kinds of handy devices like sensors, computer chips, and even machines that can shoot out a deadly beam of radiation (just kidding on that last one...I think). But the regular Hall Effect has one big downside - it only works at incredibly cold temperatures near absolute zero. Not very practical!The Anomalous Hall EffectThis is where the new Quantum Anomalous Hall Effect discovered by scientists in China comes into play. They found a way to get the same cool electron-deflecting properties of the Hall Effect, but at much higher, more realistic temperatures. And they did it using some crazy quantum physics tricks.You see, the researchers used special materials called topological insulators that have insulating interiors but highly conductive surfaces. By sandwiching these topological insulators between two layers of magnets, they were able to produce a strange quantum phenomenon.Electrons on the surface of the materials started moving in one direction without any external energy needed to keep them going! It's like they created a perpetual motion machine for electrons on a quantum scale. The spinning quantum particlesget deflected by the magnetic layers and start flowing in weird looping patterns without any resistance.Why It's So AwesomeSo why is this Quantum Anomalous Hall Effect such a big deal? A few reasons:It could lead to way more efficient electronics that don't waste energy through heat and resistance like current devices do. Just imagine a computer chip that works with virtually no power at all!The effect allows for extremely precise control over the movement of electrons, which could unlock all kinds of crazy quantum computing applications we can barely even imagine yet.It gives scientists a totally new window into understanding the bizarre quantum realm and the funky behavior of particles at that scale.The materials used are relatively inexpensive and common compared to other cutting-edge quantum materials. So this isn't just a cool novelty - it could actually be commercialized one day.Some Science Celebrities Think It's Nobel-WorthyLots of big-shot scientists around the world are going gaga over this Quantum Anomalous Hall Effect discovered by the researchers in China. A few have even said they think it deserves a Nobel Prize!Now, as cool as that would be, we have to remember that not everyone agrees it's Nobel-level just yet. Science moves slow and there's always a ton of debate over what discoveries are truly groundbreaking enough to earn that high honor.But one thing's for sure - this effect is yet another example of how China is becoming a global powerhouse when it comes to cutting-edge physics and scientific research. Those Chinese scientists are really giving their counterparts in the US, Europe, and elsewhere a run for their money!The Future is QuantumWhether the Quantum Anomalous Hall Effect leads to a Nobel or not, one thing is certain - we're entering an age where quantum physics is going to transform technology in ways we can barely fathom right now.From quantum computers that could solve problems millions of times faster than today's machines, to quantum sensors that could detect even the faintest subatomic particles,to quantum encryption that would make data unhackable, this strange realm of quantum physics is going to change everything.So pay attention, kids! Quantum physics may seem like some weird, headache-inducing mumbo-jumbo now. But understanding these bizarre quantum phenomena could be the key to unlocking all the super-cool technologies of the future. Who knows, maybe one of you reading this could even grow up to be a famous quantum physicist yourselves!Either way, keep your eyes peeled for more wild quantum discoveries emerging from China and other science hotspots around the globe. The quantum revolution is coming, and based on amazing feats like the Anomalous Hall Effect, it's going to be one heckuva ride!篇5Whoa, Dudes! You'll Never Believe the Insanely Cool Quantum Tech from China!Hey there, kids! Get ready to have your minds totally blown by the most awesome scientific discovery ever - the quantum anomalous Hall effect! I know, I know, it sounds like a bunch of big, boring words, but trust me, this stuff is straight-upmind-blowing.First things first, let's talk about what "quantum" means. You know how everything in the universe is made up of tiny, tiny particles, right? Well, quantum is all about studying those teeny-weeny particles and how they behave. It's like a whole secret world that's too small for us to see with our eyes, but scientists can still figure it out with their mega-smart brains and super-powerful microscopes.Now, let's move on to the "anomalous Hall effect" part. Imagine you're a little electron (that's one of those tiny particles I was telling you about) and you're trying to cross a busy street. But instead of just going straight across, you get pushed to the side by some invisible force. That's kind of what the Hall effect is all about - electrons getting pushed sideways instead of going straight.But here's where it gets really cool: the "anomalous" part means that these electrons are getting pushed sideways even when there's no magnetic field around! Normally, you'd need a powerful magnet to make electrons move like that, but with this new quantum technology, they're doing it all by themselves. It's like they've got their own secret superpowers or something!Now, you might be wondering, "Why should I care about some silly electrons moving around?" Well, let me tell you, thisdiscovery is a huge deal! You see, scientists have been trying to figure out how to control the flow of electrons for ages. It's kind of like trying to herd a bunch of rowdy puppies - those little guys just want to go wherever they want!But with this new quantum anomalous Hall effect, scientists in China have finally cracked the code. They've found a way to make electrons move in a specific direction without any external forces. That means they can control the flow of electricity like never before!Imagine having a computer that never overheats, or a smartphone that never runs out of battery. With this new technology, we could create super-efficient electronic devices that waste way less energy. It's like having a magical power switch that can turn on and off the flow of electrons with just a flick of a wrist!And that's not even the coolest part! You know how sometimes your electronics get all glitchy and stop working properly? Well, with this quantum tech, those problems could be a thing of the past. See, the anomalous Hall effect happens in special materials called "topological insulators," which are like super-highways for electrons. No matter how many twists andturns they take, those little guys can't get lost or stuck in traffic jams.It's like having a navigation system that's so good, you could close your eyes and still end up at the right destination every single time. Pretty neat, huh?But wait, there's more! Scientists are also exploring the possibility of using this new technology for quantum computing. Now, I know you're probably thinking, "What the heck is quantum computing?" Well, let me break it down for you.You know how regular computers use ones and zeros to process information, right? Well, quantum computers use something called "qubits," which can exist as both one and zero at the same time. It's like having a coin that's heads and tails at the same exact moment - totally mind-boggling, I know!With this quantum anomalous Hall effect, scientists might be able to create super-stable qubits that can perform insanely complex calculations in the blink of an eye. We're talking about solving problems that would take regular computers millions of years to figure out. Imagine being able to predict the weather with 100% accuracy, or finding the cure for every disease known to humankind!So, what do you say, kids? Are you as pumped about this as I am? I know it might seem like a lot of mumbo-jumbo right now, but trust me, this is the kind of stuff that's going to change the world as we know it. Who knows, maybe one day you'll be the one working on the next big quantum breakthrough!In the meantime, keep your eyes peeled for more news about this amazing discovery from China. And remember, even though science can be super complicated sometimes, it's always worth paying attention to. After all, you never know when the next mind-blowing quantum secret might be revealed!篇6Title: A Magical Discovery in the World of Tiny Particles!Have you ever heard of something called the "Quantum Anomalous Hall Effect"? It might sound like a tongue twister, but it's actually a super cool new technology that was recently discovered by scientists in China!Imagine a world where everything is made up of tiny, tiny particles called atoms. These atoms are so small that you can't see them with your bare eyes, but they're the building blocks that make up everything around us – from the chair you're sitting on to the air you breathe.Now, these atoms can do some pretty amazing things when they're arranged in certain ways. Scientists have found that if they create special materials where the atoms are arranged just right, they can make something called an "electrical current" flow through the material without any resistance!You might be wondering, "What's so special about that?" Well, let me explain! Usually, when electricity flows through a material like a metal wire, it faces something called "resistance." This resistance makes it harder for the electricity to flow, kind of like trying to run through a thick forest – it's tough and you get slowed down.But with this new Quantum Anomalous Hall Effect, the electricity can flow through the special material without any resistance at all! It's like having a wide-open road with no obstacles, allowing the electricity to zoom through without any trouble.So, how does this magical effect work? It all comes down to the behavior of those tiny atoms and the way they interact with each other. You see, in these special materials, the atoms are arranged in a way that creates a kind of "force field" that protects the flow of electricity from any resistance.Imagine you're a tiny particle of electricity, and you're trying to move through this material. As you move, you encounter these force fields created by the atoms. Instead of slowing you down, these force fields actually guide you along a specific path, almost like having a team of tiny helpers clearing the way for you!This effect was discovered by a group of brilliant scientists in China, and it's considered a huge breakthrough in the field of quantum physics (the study of really, really small things). It could lead to all sorts of amazing technologies, like super-fast computers and more efficient ways to transmit electricity.But that's not all! This discovery is also important because it proves that China is at the forefront of cutting-edge scientific research. The scientists who made this discovery are being hailed as potential Nobel Prize winners, which is one of the highest honors a scientist can receive.Isn't it amazing how these tiny, invisible particles can do such incredible things? The world of science is full ofmind-blowing discoveries, and the Quantum Anomalous Hall Effect is just one example of the amazing things that can happen when brilliant minds come together to explore the mysteries of the universe.So, the next time you hear someone mention the "Quantum Anomalous Hall Effect," you can proudly say, "Oh, I know all about that! It's a magical discovery that allows electricity to flow without any resistance, and it was made by amazing Chinese scientists!" Who knows, maybe one day you'll be the one making groundbreaking discoveries like this!。

松材线虫效应蛋白Bx-FAR-1的原核表达与活性测定

松材线虫效应蛋白Bx-FAR-1的原核表达与活性测定

·2731·收稿日期:2020-05-12基金项目:国家重点研发计划项目(2018YFD0600203);江苏高校优势学科建设工程项目(PAPD )作者简介:*为通讯作者,吴小芹(1957-),教授,主要从事森林微生物与森林病理研究工作,E-mail :*************.cn 。

李煜(1995-),研究方向为森林病理,E-mail :******************松材线虫效应蛋白Bx-FAR-1的原核表达与活性测定李煜1,2,吴小芹1,2*,胡龙娇1,2(1南京林业大学南方现代林业协同中心/南京林业大学林学院,南京210037;2江苏省有害生物入侵预防与控制重点实验室,南京210037)摘要:【目的】纯化获得松材线虫效应蛋白Bx-FAR-1并验证其体外活性,为后续研究效应蛋白Bx-FAR-1参与松材线虫侵染松树的分子机制打下基础。

【方法】利用PCR 技术从松材线虫cDNA 中扩增出效应基因Bx-F AR-1,用同源重组的方式将目的片段连接至原核表达载体pET-32a (+)。

通过原核表达系统诱导并纯化获得大量效应蛋白Bx-FAR-1,利用SDS-聚丙烯酰氨凝胶电泳(SDS-PAGE )和Western blotting 对纯化获得的蛋白进行鉴定,同时通过本氏烟瞬时表达系统验证蛋白活性。

【结果】从松材线虫cDNA 中成功扩增出Bx-F AR-1基因(BXY_1685000.1);通过同源重组成功获得重组表达载体pET32a-BxFAR ,并转化至大肠杆菌BL21感受态细胞。

当诱导条件为15℃下150r/min 诱导16h 时重组蛋白以可溶性形式表达,且蛋白表达量高。

效应蛋白Bx-FAR-1活性测定结果显示,当蛋白浓度达100nmol/L 时,可抑制致病疫霉的病原相关分子模式(PAMP )INF1引起的烟草细胞坏死。

【结论】松材线虫效应蛋白Bx-FAR-1可抑制寄主植物的免疫反应。

219401825_UHPLC-Q-TOF-MS

219401825_UHPLC-Q-TOF-MS

马博稷,肖岩,陈祖德,等. UHPLC-Q-TOF-MS/MS 分析青钱柳嫩叶渗漉提取液化学成分[J]. 食品工业科技,2023,44(13):281−291. doi: 10.13386/j.issn1002-0306.2022070294MA Boji, XIAO Yan, CHEN Zude, et al. Analysis of Chemical Constituents in Percolate the Extract of Cyclocarya paliurus Tender Leaves by UHPLC-Q-TOF-MS/MS[J]. Science and Technology of Food Industry, 2023, 44(13): 281−291. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070294· 分析检测 ·UHPLC-Q-TOF-MS/MS 分析青钱柳嫩叶渗漉提取液化学成分马博稷1,肖 岩1,陈祖德1,舒任庚2,李冰涛1,3,姜 丽1,3,4,徐国良1,3,4, *,张启云1,3,*(1.江西中医药大学中医基础理论分化发展研究中心,江西南昌 330004;2.江西中医药大学药学院,江西南昌 330004;3.江西省中医病因生物学重点实验室,江西南昌 330004;4.江西省中药药理学重点实验室,江西南昌 330004)摘 要:为分析青钱柳嫩叶的化学成分,本研究采取高效液相色谱-四级杆飞行时间串联质谱法(UHPLC-Q-TOF-MS/MS )对青钱柳嫩叶渗漉提取液的化学成分进行快速定性分析。

在正、负离子模式下全扫描和对母离子电子轰击,根据各化合物的精确分子量及元素组成、主要碎片信息、保留时间以及结合青钱柳相关文献和数据库检索,从青钱柳中共鉴定出94种化合物,包括29种黄酮类、16种三萜类、25种有机酸类、24种其他类化合物。

scientifically-documented results 科学

scientifically-documented results 科学

Scientifically-documented results 科学,即科学文献中的科学成果。

这些成果是通过科学方法得出的,经过严格的实验和验证,并被记录在科学文献中。

这些科学成果对于推动科学研究和理解自然现象具有重要意义。

在科学研究中,科学家们通常会通过实验、观察、测量和分析等方法来收集数据,并使用这些数据来得出结论。

这些结论需要经过同行评审和验证,以确保其准确性和可靠性。

一旦这些结论被确认,它们就会被记录在科学文献中,成为科学知识的一部分。

科学文献中的科学成果对于推动科学研究和理解自然现象具有重要意义。

它们可以帮助科学家们验证和证实自己的假设和理论,也可以帮助科学家们发现新的现象和规律。

此外,科学文献还可以为政府、企业和个人提供决策和行动的依据,以促进科技进步和社会发展。

总之,Scientifically-documented results 科学是科学研究中得出的经过严格验证和记录的成果,对于推动科学研究和理解自然现象具有重要意义。

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Fig. 2 shows the comparison of a direct HST WFPC2 image with several monochromatic maps and the continuum. The spectrum in Fig. 3 was created from the combined datacube by defining a digital aperture and co-adding a total of 62 spatial elements (”spaxels”) within this aperture, each measuring 0.5 x 0.5 arcsec squared. The roughly elliptical aperture was adapted to the elongated region of significant surface brightness along the NW direction. The spectrum shows a rich emission line spectrum, including the Balmer series, the forbidden lines of [O III] 5007,4959 and [O III] 4363, [O II] 3727,3729, He I 3889, and He II 4686, in good agreement with the spectra published by Melnick et al. (1992).
1. Introduction
PMAS1 is a dedicated 3D instrument with a 16×16 square element IFU (0.5 arcsec pitch), fiber-coupled to a fully refractive fiber spectrograph, which is based on CaF2 lenses and has good response in the blue. It is currently equipped with a 2K×4K thinned CCD (SITe ST002A), providing 2048 spectral bins. A 2×2K×4K mosaic CCD, which was commissioned 2003, increases the free spectral range to 4096 spectral bins. The present fiber bundle has been conservatively manufactured with 100µm diameter, high OH− doped fibers for good UV transmission. A future upgrade with 5060µm diameter fibers is intended to replace the existing IFU with a 32×32 element array. A unique feature of PMAS is the internal A&G camera, equipped with a LN2 -cooled, bluesensitive SITe TK1024 CCD, giving images with a scale of 0.2 arcsec/pixel and a FOV of 3.4×3.4 arcmin2. The camera can be used with various broad-band and narrow-band filters. For a more detailed description, see Roth et al. 2000a and Kelz et al. 2003. After First Light in May 2001, a Science Verification run was conducted at the Calar Alto 3.5m Telescope in October 2001. Since then the instrument is available at this telescope as a common user instrument. In this paper, we describe our first results from the Science Verification observations. We selected targets with well-known properties from the literature in order to assess whether PMAS is capable of reproducing these data.
Fig. 3. Co-added spectrum of SBS0335-052 from spaxels within the roughly elliptical region of high surface brightness in Fig. 2. ˚ ing, which provided a spectral resolution of 1.4 A FWHM and ˚ a wavelength coverage of 4450–5150 A. In accord with the results of Pakull & Mirioni (2002), we obtain maps in [O III] ˚ ˚ 5007 A, [O III] 4959 A, and Hβ , and the spectrum is shown in Fig. 4. The He II emission is constrained to a region to the left of the insert map, coinciding with a Chandra detection of Holmberg II X-1 within the error circle . The total flux in this emission line is 6×10−17erg/cm2 /sec. There is also a hint of a faint continuum, increasing towards the blue. A more detailed analysis is presented by Lehmann et al. (in prep.).
Fig. 1. PMAS at the Cassegrain focus of the 3.5m Telescope at Calar Alto Observatory, Spain.
2. SBS0335-052
The blue compact dwarf galaxy SBS0335-052 is the second most metal-poor known galaxy after I Zw18, and thus an interesting target for spectrophotometric observations. Its oxygen abundance is 41 times lower than solar. It is thought to contain 6 embedded star clusters with a significant number of supermassive stars of around 100 solar masses (Thuan et al. 1997). The intense far UV radiation of those stars leads to high excitation ionization of the associated H II regions, showing electron temperatures as high as 25000 K. The emission line spectrum of this galaxy has been studied sufficiently
Correspondence to: mmroth@aip.de 1 http://www.aip.de/groups/opti/pmas/OptI pmas.html
A.N. Author: Title
1
well in the literature, providing a good test case for the PMAS science verification observations. We observed SBS0335-052 on October 25, 2001, using the V600 grating, which yields a reciprocal dispersion of ˚ 0.8 A/pixel. In the 2×2 binned readout mode of the spectrograph CCD, which was used throughout this campaign, ˚ the dispersion was thus 1.6 A/bin. The spectral resolution ˚ was 3.3 A FWHM. The grating angle was set to cover a ˚ wavelength range of 3600–5200 A. We took 7 exposures of 900sec each over a range in airmass of 1.69. . .1.35. The conditions were non-photometric with a seeing of about 1.5arcsec FWHM.
A00X) X, XXX–XXX
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