Interaction-induced delocalization of two particles in a random potential Scaling propertie

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分析三联、四联药物方案治疗胃溃疡的临床效果

分析三联、四联药物方案治疗胃溃疡的临床效果

系统医学 2023 年 12 月第 8 卷第 24期分析三联、四联药物方案治疗胃溃疡的临床效果王昌盛1,陈兰2,廖小红21.广东药科大学附属第一医院药学部,广东广州510062;2.广东三九脑科医院药剂科,广东广州510510[摘要]目的探讨胃溃疡患者选择四联药物治疗后的临床效果。

方法选取2022年1月—2023年8月广东药科大学附属第一医院收治的76例胃溃疡患者为研究对象,依据投掷硬币法分组,参照组(38例)选择三联药物治疗,研究组(38例)选择四联药物治疗,比较两组治疗总有效率、胃灼痛评分、胃溃疡面积、上腹疼痛评分、临床症状改善时间。

结果研究组治疗总有效率为97.37%,明显高于参照组,差异有统计学意义(χ2= 6.176,P<0.05)。

治疗后,研究组胃灼痛评分、胃溃疡面积、上腹疼痛评分、临床症状改善时间均低于参照组,差异有统计学意义(P均<0.05)。

结论同三联药物比较,胃溃疡患者接受四联药物治疗,可显著提升临床效果,有效改善疾病症状,可促进胃溃疡患者的良好预后。

[关键词]胃溃疡;三联药物;四联药物;疗效[中图分类号]R573 [文献标识码]A [文章编号]2096-1782(2023)12(b)-0175-03 Clinical Effect of Triple and Quadruple Drug Regimens in the Treatment of Gastric UlcerWANG Changsheng1, CHEN Lan2, LIAO Xiaohong21.Department of Pharmacy, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guang⁃dong Province, 510062 China;2.Department of Pharmacy, Guangdong Sanjiu Brain Hospital, Guangzhou, Guangdong Province, 510510 China[Abstract] Objective To investigate the clinical effect of quadruple drug therapy in patients with gastric ulcer. Methods Seventy-six patients with gastric ulcer admitted to the First Affiliated Hospital of Guangdong Pharmaceuti⁃cal University from January 2022 to August 2023 were selected as the research object and divided into groups ac⁃cording to coin tossing method. The reference group (38 cases) received triple drug therapy, and the study group (38 cases) received quadruple drug therapy. The total effective rate, the score of heartburn pain, the area of gastric ulcer, the score of upper abdominal pain and the improvement time of clinical symptoms were compared between the two groups. Results The total effective rate of the study group was 97.37%, which was significantly higher than that of the reference group, and the difference was statistically significant (χ2=6.176, P<0.05). After treatment, the score of heartburn pain, the area of gastric ulcer, the score of upper abdominal pain and the improvement time of clinical symptoms in the study group were lower than those in the reference group, and the differences were statistically sig⁃nificant (all P<0.05). Conclusion Compared with triple drug, quadruple drug treatment for gastric ulcer patients can significantly improve the clinical effect, effectively improve the disease symptoms, and promote the good prognosis of patients with gastric ulcer.[Key words] Gastric ulcer; Triple drug; Quadruple drugs; Curative effect对于胃溃疡疾病而言,其属于一种胃肠道高发病[1-2]。

【盘点】Label-free近期高分文献汇总

【盘点】Label-free近期高分文献汇总

【盘点】Label-free近期高分文献汇总非标定量法(Label-Free)是一种非常重要的质谱定量方法,通过比较质谱分析次数或质谱峰强度,分析不同来源样品蛋白的数量变化。

该技术操作简单,无需昂贵的同位素标签做标记,可用于大规模数量样本检测,从而广受科研工作者的推崇。

近年来,应用Label-free技术的文献数量一直呈上升趋势,出现在Nature、Cell等顶级刊物上的文章也层出不穷,且表现出大规模样品蛋白质组学多用Label-free技术的态势。

这里,小编为大家盘点了几篇近期发表使用Label-free定量蛋白质组学方法的高分文献,供大家参考。

Proteomics identifies new therapeutic targets of early-stage hepatocellular carcinoma. Transplantation.2019 May.该研究通过对中山医院手术切除的肝细胞癌及配对癌旁组织进行蛋白组学和磷酸组学分析,提出早期肝细胞癌的蛋白质组学分层方法,并发现一种称作甾醇O-酰基转移酶1(SOAT1)其表达水平与肝癌患者较差的预后密切相关。

研究从蛋白组学角度发现可疑肿瘤相关蛋白,找到了肝细胞癌精准治疗的潜在新靶点,为发展新型抗癌药物提供了重要基础。

NUFIP1 is a ribosome receptor for starvation-induced ribophagy. Science. 2018 May.mTORC1和AMPK作为主要代表的能量感受调节器,和溶酶体具有非常亲密的联系。

本研究对快速分离的溶酶体进行定量蛋白质组分析,发现营养水平和mTOR可对溶酶体蛋白质组进行动态调节。

抑制mTORC1后,NUFIP1重分布到自噬体和溶酶体,并与核糖体相互作用,通过与微管相关蛋白1A/1B轻链3的直接结合将其转运到自噬体从而使其降解,提示NUFIP1是选择性核糖体自噬的受体。

芳香性及其判断规则

芳香性及其判断规则

4
Recommendation
Aromaticity
Michael Faraday discovered benzene in 1825

Chemists in the early 19th century first just used the term “aromatic”
Aromatic compounds are more stables often far
currents or “aromaticity”, whereas positive values (i.e., deshielded) at each
point denote paratropic ring currents and “antiaromaticity”.
Negative
Interior positions
P. v. R. Schleyer, and F. Puhlhofer, Org. Lett. 4, 2873 (2002)
Magnetic criteria of aromaticity
Ring currents
Nucleus-Independent Chemical Shifts(NICS)
Ring Currents
NICS
P. v. R. Schleyer et al., J. Am. Chem. Soc. 118, 6317 (1996). 200 180 160 140 120 100 80 60 40 20 0
Record Count
The ability to sustain a diatropic ring current is the defining characteristic of aromatic species. Cyclic electron delocalization results in enhanced stability, bond length equalization,and special magnetic as well as chemical and physical properties.

分子生物学名词解释大全

分子生物学名词解释大全

分子生物学名词解释大全AAbundance (mRNA 丰度):指每个细胞中mRNA 分子的数目。

Abundant mRNA(高丰度mRNA):由少量不同种类mRNA组成,每一种在细胞中出现大量拷贝。

Acceptor splicing site (受体剪切位点):内含子右末端和相邻外显子左末端的边界。

Acentric fragment(无着丝粒片段):(由打断产生的)染色体无着丝粒片段缺少中心粒,从而在细胞分化中被丢失。

Active site(活性位点):蛋白质上一个底物结合的有限区域。

Allele(等位基因):在染色体上占据给定位点基因的不同形式。

Allelic exclusion(等位基因排斥):形容在特殊淋巴细胞中只有一个等位基因来表达编码的免疫球蛋白质。

Allosteric control(别构调控):指蛋白质一个位点上的反应能够影响另一个位点活性的能力。

Alu-equivalent family(Alu 相当序列基因):哺乳动物基因组上一组序列,它们与人类Alu家族相关。

Alu family (Alu家族):人类基因组中一系列分散的相关序列,每个约300bp长。

每个成员其两端有Alu 切割位点(名字的由来)。

α-Amanitin(鹅膏覃碱):是来自毒蘑菇Amanita phalloides 二环八肽,能抑制真核RNA聚合酶,特别是聚合酶II 转录。

Amber codon (琥珀MM子):核苷酸三联体UAG,引起蛋白质合成终止的三个MM子之一。

Amber mutation (琥珀突变):指代表蛋白质中氨基酸MM子占据的位点上突变成琥珀MM子的任何DNA 改变。

Amber suppressors (琥珀抑制子):编码tRNA的基因突变使其反MM子被改变,从而能识别UAG MM子和之前的MM子。

Aminoacyl-tRNA (氨酰-tRNA):是携带氨基酸的转运RNA,共价连接位在氨基酸的NH2基团和tRNA 终止碱基的3¢或者2¢-OH 基团上。

物理化学-专业词汇中英文对照表

物理化学-专业词汇中英文对照表

非自发过程 non-spontaneous
平衡 equilibrium 热机 heat engine
不可逆 irreversible 可逆 reversible
3.2 卡诺循环和卡诺定理 Carnot cycles and Carnot’Law
热机效率 efficiency of the heat engine
8.4 可逆电池和可逆电极 Reversible cell and electrode
国际理论和应用化学联合会 IUPAC(International Union of Pure and Applied Chemistry)
化学电源 electrochemical cell
8.5 可逆电池热力学 reversible cell thermodynamics
Carnot 定理 Carnot law
可逆热机 revisable engine
不可逆热机 irreversible engine
冷冻系数 freezing coefficient
3.3 熵的概念 the concept of entropy
熵 entropy
克劳修斯不等式 Clausius inequality
Ideal dilute solution
4.7 相对活度的概念 relative activity
4.8 稀溶液的依数性 colligative properties of the dilute solution
依数性 colligative properties
渗透压 osmotic pressure
第 7 章 化学反应动力学 Chemical Reaction kinetics
7.1 动力学的基本概念 basic concepts of kinetics

酚类有机物光催化氧化机理

酚类有机物光催化氧化机理

酚类有机物光催化氧化机理Phenolic compounds are a class of organic compounds that contain a hydroxyl (-OH) group bonded directly to an aromatic ring. These compounds have attracted considerable attention in recent years due to their diverse chemical structures and wide range of applications, including as antioxidants, pharmaceuticals, and UV blockers. Additionally, phenolic compounds have also shown potential for use in various environmental applications, such as wastewater treatment and pollutant degradation.酚类有机物是一类含有羟基(-OH)与芳香环直接相连的有机化合物。

由于其多样的化学结构和广泛的应用领域,这些化合物近年来引起了人们的极大关注。

它们可以作为抗氧化剂、药物和紫外线阻挡剂,并在废水处理和污染物降解等各种环境应用中显示出潜力。

One interesting aspect of phenolic compounds is their photochemical reactivity under light irradiation. Phenolic compounds can undergo light-induced oxidation reactions through the mechanism of photocatalysis. Photocatalysis refers to a process in which a catalyst is activated bylight energy to initiate a chemical reaction. In the caseof phenolic compounds, these reactions typically involvethe transfer of electrons from the phenolic compound to an electron acceptor, leading to the formation of highly reactive radicals.酚类化合物的一个有趣之处在于它们在光照下发生光催化氧化反应的光化学反应性。

Plant-Microbe Interactions in the Rhizosphere

Plant-Microbe Interactions in the Rhizosphere

Plant-Microbe Interactions in theRhizospherePlant-microbe interactions in the rhizosphere are a crucial aspect of the soil ecosystem, playing a significant role in plant growth, nutrient uptake, andoverall soil health. The rhizosphere is the narrow region of soil that is directly influenced by the roots of plants, where a complex network of interactions occurs between the plant, soil, and various microorganisms. These interactions can beboth beneficial and detrimental, depending on the specific microorganisms involved and the environmental conditions. Understanding the dynamics of plant-microbe interactions in the rhizosphere is essential for developing sustainableagricultural practices and improving crop productivity. One of the most important aspects of plant-microbe interactions in the rhizosphere is the exchange of nutrients between the plant and the microorganisms. Plants release a variety of compounds, such as sugars, amino acids, and organic acids, into the rhizosphere through their roots. These compounds serve as an energy source for the diverse microbial community in the soil, including bacteria, fungi, and archaea. In return, the microorganisms help the plant acquire essential nutrients, such as nitrogen, phosphorus, and iron, by solubilizing and mineralizing soil nutrients, making them more available for plant uptake. This mutualistic relationship between plants and microorganisms is crucial for the overall health and productivity of the plant.In addition to nutrient exchange, plant-microbe interactions in the rhizosphere also play a vital role in plant defense against pathogens. Certain microorganismsin the rhizosphere, known as plant growth-promoting rhizobacteria (PGPR), have been shown to stimulate plant growth and enhance resistance to diseases. These beneficial microorganisms can directly inhibit the growth of plant pathogens by producing antimicrobial compounds or competing for space and resources in the rhizosphere. Furthermore, PGPR can also induce systemic resistance in plants, activating their defense mechanisms against a wide range of pathogens. Understanding the mechanisms by which PGPR confer disease resistance to plants can have significant implications for reducing the reliance on chemical pesticides in agriculture. However, not all plant-microbe interactions in the rhizosphere arebeneficial. Some microorganisms can have detrimental effects on plant health, causing diseases and reducing crop yields. For example, soil-borne pathogens, such as Fusarium and Phytophthora species, can infect plant roots and cause root rot, leading to stunted growth and wilting of the plant. These pathogenic microorganisms can outcompete beneficial microbes in the rhizosphere, disrupting the delicate balance of the soil ecosystem. Understanding the factors that contribute to the proliferation of pathogenic microorganisms in the rhizosphere is essential for developing effective strategies to manage plant diseases and maintain soil health. Moreover, the composition and diversity of the microbial community in the rhizosphere are influenced by various factors, including soil type, plant species, and environmental conditions. Different plants release different types and amounts of root exudates, which can selectively promote the growth of specific groups of microorganisms in the rhizosphere. Furthermore, the physical and chemical properties of the soil, such as pH, moisture, and organic matter content, can also have a significant impact on the structure and function of the rhizosphere microbial community. Understanding the complex interplay between these factors and their effects on plant-microbe interactions is crucial for optimizing soil management practices and promoting sustainable agriculture. In conclusion, plant-microbe interactions in the rhizosphere are a dynamic and intricate network of relationships that profoundly impact plant growth, nutrient cycling, and soil health. The exchange of nutrients, the promotion of plant defense mechanisms, and the influence of environmental factors all contribute to the complexity of these interactions. By gaining a deeper understanding of the mechanisms underlying plant-microbe interactions in the rhizosphere, we can develop innovative strategies to enhance crop productivity, reduce the reliance on chemical inputs, and promote sustainable agricultural practices. Ultimately, this knowledge can contribute to the development of a more resilient and environmentally friendly agricultural system, benefiting both farmers and the broader ecosystem.。

一种新型混合模式固定相用于指甲油中邻苯二甲酸酯类物质的检测

一种新型混合模式固定相用于指甲油中邻苯二甲酸酯类物质的检测

基金项目:国家自然科学基金青年项目(21804112)通信作者:王路军,E-mail:************.cn 引用本文:李志敏,阮乐平,周静秋,等.一种新型混合模式固定相用于指甲油中邻苯二甲酸酯类物质的检测[J].西南医科大学学报。

2023,46(5):404-407.DOI:10.3969/j.issn.2096-3351.2023.05.007高效液相色谱(high performance liquid chromatog⁃raphy ,HPLC )是最常用的化合物分离和纯化技术之一[1]。

在传统色谱分离过程中,分析物和固定相只有一种形式的作用存在,这种单一模式色谱柱往往在分离时还具有一定的局限性[2]。

混合模式高效液相色谱(mixed-mode high-performance liquid chromatography ,MHPLC )是将多功能的固定相填料填充色谱柱,在固定相和流动相之间具有不止一种形式的相互作用,可在单个色谱柱中同时高效分离和纯化不同类型化合物[3-8]。

MHPLC 具有高选择性、高分辨率和高负载能力等特点,这对分离复杂体系样品具有重要意义[1]。

点击化学反应是合成混合模式固定相材料的一种新方法,具有高效、无副反应的优点,因此基于点击化学反应合成新型的色谱固定相已经成为研究的热点之一[9-12]。

指甲油中常见的毒性物质主要有邻苯二甲酸酯类(phthalic acid esters ,PAEs )、甲苯和甲醛等,但关于指甲油中PAEs 的含量测定,目前还很少有人研究[13-14]。

一种新型混合模式固定相用于指甲油中邻苯二甲酸酯类物质的检测李志敏,阮乐平,周静秋,代雪梅,王路军西南医科大学药学院(泸州646000)【摘要】目的利用合成的新型固定相填料(命名为Sil-styrene )填充色谱柱,建立混合模式高效液相色谱分析方法,将其用于指甲油中邻苯二甲酸酯类(phthalic acid esters,PAEs )物质的检测。

alevel bonding指化学键和分子间作用力

alevel bonding指化学键和分子间作用力

alevel bonding指化学键和分子间作用力A-level bonding refers to the various types of chemical bonds and intermolecular forces that are studied at the A-level (advanced level) of chemistry education. These include:1. Ionic bonding: This occurs when there is a transfer of electrons between atoms, resulting in the formation of positive and negative ions. The attraction between these ions is what holds the bond together. Ionic bonds are typically found in compounds that involve metals andnon-metals.2. Covalent bonding: In covalent bonding, atoms share electrons to forma bond. This can occur between atoms of the same element or different elements. Covalent bonds are typically found in molecules and can be classified as either polar or non-polar, depending on the electronegativity difference between the atoms involved.3. Metallic bonding: This type of bonding is found in metals. It involves the delocalization of valence electrons, which creates a "sea" of electrons that hold the metal ions together. Metallic bonding is responsible for the characteristic properties of metals, such as their high electrical and thermal conductivity and malleability.In addition to these types of chemical bonding, A-level chemistry also covers intermolecular forces, which are the forces of attraction between molecules. These forces include:1. London dispersion forces: These are the weakest intermolecular forces and are caused by temporary fluctuations in electron distribution within molecules. London dispersion forces are present in all molecules, but they become stronger with increasing molecular size and shape.2. Dipole-dipole interactions: These occur between polar molecules and are caused by the attraction between the positive end of one molecule and the negative end of another molecule. Dipole-dipole interactions are stronger than London dispersion forces.3. Hydrogen bonding: This is a special type of dipole-dipole interaction that occurs when a hydrogen atom is bonded to a highly electronegative atom (such as oxygen, nitrogen, or fluorine) and is attracted to another electronegative atom. Hydrogen bonding is responsible for many of the unique properties of substances like water.Overall, A-level bonding encompasses the study of chemical bondswithin molecules and the intermolecular forces that operate between molecules. Understanding these bonding forces is crucial for understanding the properties and behavior of substances in chemistry.。

有机无机分子的芳香性

有机无机分子的芳香性
• Reactivity – lower reactivity, electrophilic aromatic substitution (neither direct nor reliable!)
• Spectroscopic– UV, proton chemical shifts, magnetic susceptibility exaltation (indirect, mostly reliable, but sometimes impractical!)
2) Aromaticity is not a physical observable, having no precise experimental definition.
3) Aromaticity is just like to define beauty in our daily life!
有机无机分子的芳香性
Outline
❖ Overview ❖ Practical Criteria of Aromaticity ❖ p-aromaticity (2D) ❖ Möbius aromaticity ❖ Homoaromaticity ❖ s-aromaticity ❖ Spherical aromaticity and 3D aromaticity
CunHn (n=4,5,6)
M4Li2 (M=Cu,Ag, Au)
1) Tsipis et al. J. Am. Chem. Soc. 2003, 125, 1136. 2) Schleyer et al. J. Am. Chem. Soc. 2005, 127, 5701.
1.2 Main developments about aromaticity

镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响

镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响

镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响胡君茹;姜华【摘要】Objective: To explore the influence of flavonoid glycosides extracted from Tibetan medicine LianXing JiDou (Oxytropis falcata Bunge) on the cytokines of the immunosuppressive mice. Methods: Sixty mice were randomized into six groups: the blank group, the model group, high, moderate and low dose groups of flavonoid glycosides extracted from LianXing JiDou (336, 168, 84 mg/kg) as well as levamisole hydrochloride group (25 mg/kg), ten mice each group. High, moderate and low dose groups of flavonoid glycosides extracted from LianXing JiDou accepted intragastric administration of flavonoid glycosides in different doses respectively; the blank group and the model group were drenched with the same amount of 5% tween-80 solutions, for ten days. The immunosuppressive mice models were prepared by injecting cyclophosphamide since the eighth day, except the blank group received peritoneal injection of physiological saline, other groups accepted peritoneal injection of cyclophosphamide for three days. The contents of IL-1, IL-2, IL-4, IL-8, IL-10, TNF-α and IFN-γ in the serum and the contents of IL-2, IL-4, IL-10, TNF-α and IFN-γ in the supernatant of cultured splenic lymphocytes were detected by using ELISA method, to observe the influence of flavonoid glycosides extracted from Tibetan medicine LianXing JiDou on the cytokines of the immunosuppressive mice. Results: Compared with the model group, different doses groups of flavonoid glycosidesextracted from LianXing JiDou could effectively raise the contents of cytokines in blood serum and the supernatant of cultured splenic lymphocytes of the immunosuppressive mice, and the difference had statistical meaning (P<0.05). Conclusion: Flavonoid glycosides extracted from LianXing JiDou could resist cyclophosphamide-induced immunosuppressive action of the mice, and improve the immunologic function of the mice.%目的:研究藏药镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响.方法:将60只昆明种小鼠随机分为6组:空白组,模型组,镰形棘豆黄酮苷元高、中、低剂量组(336、168、84 mg/kg)和盐酸左旋咪唑组(25 mg/kg),每组10只.镰形棘豆黄酮苷元高、中、低剂量组分别灌胃不同剂量的黄酮苷元;盐酸左旋咪唑组灌胃左旋咪唑25 mg/kg;空白组和模型组灌服等体积5%吐温-80溶液,共10天.第8天开始注射环磷酰胺制备免疫低下小鼠模型,除空白组腹腔注射生理盐水外,其他各组分别腹腔注射环磷酰胺,共3天.采用酶免仪分别测定血清中白细胞介素1、2、4、8、10(IL-1、IL-2、IL-4、IL-8、IL-10),肿瘤坏死因子α(TNF-α),干扰素γ(IFN-γ)的含量及脾淋巴细胞培养上清液中IL-2、IL-4、IL-10、TNF-α、IFN-γ的含量,观察镰形棘豆黄酮苷元对免疫抑制小鼠细胞因子的影响.结果:与模型组比较,镰形棘豆黄酮苷元各剂量组能有效升高免疫抑制小鼠血清和脾淋巴细胞培养液中各细胞因子的含量,差异有统计学意义(P<0.05).结论:镰形棘豆黄酮苷元能够拮抗环磷酰胺所致小鼠的免疫抑制作用,增强小鼠的免疫功能.【期刊名称】《西部中医药》【年(卷),期】2017(030)011【总页数】3页(P12-14)【关键词】镰形棘豆黄酮苷元;细胞因子;免疫抑制;环磷酰胺【作者】胡君茹;姜华【作者单位】甘肃省中医药研究院,甘肃兰州 730050;甘肃省中医药研究院,甘肃兰州 730050【正文语种】中文【中图分类】R285.5镰形棘豆(Oxytropis falcata Bunge)为豆科棘豆属植物,主要产于青海、甘肃南部、四川西部等[1]。

会议通知|2022年亚太肝病学会肝纤维化专题会

会议通知|2022年亚太肝病学会肝纤维化专题会

[J].BestPractResClinGastroenterol,2011,25(2):195-206.DOI:10.1016/j.bpg.2011.02.005.[2]MEDERACKEI,HSUCC,TROEGERJS,etal.Fatetracingrevealshepaticstellatecellsasdominantcontributorstoliverfi brosisindependentofitsaetiology[J].NatCommun,2013,4:2823.DOI:10.1038/ncomms3823.[3]RAYK.Liver:hepaticstellatecellsholdthekeytoliverfibrosis[J].NatRevGastroenterolHepatol,2014,11(2):74.DOI:10.1038/nrgastro.2013.244.[4]PIERA-VELAZQUEZS,MENDOZAFA,JIMENEZSA.Endo thelialtomesenchymaltransition(EndoMT)inthepathogene sisofhumanfibroticdiseases[J].JClinMed,2016,5(4):45.DOI:10.3390/jcm5040045.[5]SUNX,NKENNORB,MASTIKHINAO,etal.Endothelium-mediatedcontributionstofibrosis[J].SeminCellDevBiol,2020,101:78-86.DOI:10.1016/j.semcdb.2019.10.015.[6]RUANB,DUANJL,XUH,etal.Capillarizedliversinusoidalendothelialcellsundergopartialendothelial-mesenchymaltransitiontoactivelydepositsinusoidalECMinliverfibrosis[J].FrontCellDevBiol,2021,9:671081.DOI:10.3389/fcell.2021.671081.[7]THOMSONJG,RUCKEREB3rd,PIEDRAHITAJA.MutationalanalysisofloxPsitesforefficientCre-mediatedinsertionintogenomicDNA[J].Genesis,2003,36(3):162-167.DOI:10.1002/gene.10211.[8]KOSCH.Cre/loxPsystemforgeneratingtissue-specificknockoutmousemodels[J].NutrRev,2004,62(6Pt1):243-246.DOI:10.1301/nr2004.jun243-246.[9]JIANGZJ,YUEZS,YANGYC,etal.ImprovementmethodofisolationofmouseliverSinusoidalendothelialcell[J].ProgModBiomed,2018,18(6):1034-1039.DOI:10.13241/j.cnki.pmb.2018.06.007.蒋子剑,岳振生,杨毅聪,等.小鼠肝血窦内皮细胞分离与鉴定新方法[J].现代生物医学进展,2018,18(6):1034-1039.DOI:10.13241/j.cnki.pmb.2018.06.007.[10]WANGS,FRIEDMANSL.Hepaticfibrosis:Aconvergentre sponsetoliverinjurythatisreversible[J].JHepatol,2020,73(1):210-211.DOI:10.1016/j.jhep.2020.03.011.[11]TERKELSENMK,BENDIXENSM,HANSEND,etal.Tran scriptionaldynamicsofhepaticsinusoid-associatedcellsafterliverinjury[J].Hepatology,2020,72(6):2119-2133.DOI:10.1002/hep.31215.[12]TRAUTWEINC,FRIEDMANSL,SCHUPPAND,etal.Hepaticfibrosis:Concepttotreatment[J].JHepatol,2015,62(1Suppl):S15-S24.DOI:10.1016/j.jhep.2015.02.039.[13]ZEISBERGEM,TARNAVSKIO,ZEISBERGM,etal.Endothe lial-to-mesenchymaltransitioncontributestocardiacfibrosis[J].NatMed,2007,13(8):952-961.DOI:10.1038/nm1613.[14]ZEISBERGEM,POTENTASE,SUGIMOTOH,etal.Fibro blastsinkidneyfibrosisemergeviaendothelial-to-mesenchymaltransition[J].JAmSocNephrol,2008,19(12):2282-2287.DOI:10.1681/ASN.2008050513.[15]HASHIMOTON,PHANSH,IMAIZUMIK,etal.Endothelial-mesenchymaltransitioninbleomycin-inducedpulmonaryfibrosis[J].AmJRespirCellMolBiol,2010,43(2):161-172.DOI:10.1165/rcmb.2009-0031OC.引证本文:XUH,RUANB,LIZW,etal.Establishmentofamousemodelofvascularendothelial-mesenchymaltransdifferentiationgenetictracinganditsroleinliverfibrosisstudies[J].JClinHepatol,2022,38(4):832-836.许皓,阮柏,历志文,等.血管内皮间质样转分化遗传示踪小鼠模型的构建及其在肝纤维化研究中的应用[J].临床肝胆病杂志,2022,38(4):832-836.(本文编辑:刘晓红)·国内外会议动态·会议通知|2022年亚太肝病学会肝纤维化专题会APASLSTC2022:Liverfibrosisintheeraoftranslationalmedicine 会议时间:2022年6月3-5日会议地点:中国北京会议网站:http://www.apaslstc.com会议主席:尤红(首都医科大学附属北京友谊医院),徐小元(北京大学第一医院),贾继东(首都医科大学附属北京友谊医院)本次会议主题为“肝纤维化转化研究”,将邀请相关领域国际学术权威和专家从肝纤维化发病机制、最新诊疗技术以及慢性肝病病因治疗、抗纤维化新药临床试验进展等方面开展探讨,分享国内外学者关于肝纤维化和肝硬化的新药研发以及最新诊治管理策略的专业理念和创新技术。

ADAMTSL5与银屑病

ADAMTSL5与银屑病

ADAMTSL5与银屑病发表时间:2018-04-19T13:10:31.387Z 来源:《医药前沿》2018年4月第12期作者:袁育林杨霞芳[导读] 可以成为银屑病中产生IL-17的CD8+ T细胞的活化抗原。

对ADAMTSL5的深入研究为阐明银屑病发病机制及靶向治疗带来了新希望。

(南宁市广西壮族自治区人民医院检验科广西南宁 530021)【中图分类号】R758.63 【文献标识码】A 【文章编号】2095-1752(2018)12-0014-03银屑病是一种常见的慢性复发性炎症性皮肤病。

其发病机制非常复杂,包括遗传、环境、免疫等多种因素参与其中。

虽然基于广泛的遗传,免疫和药理学证据,T细胞在银屑病发病机制中的作用已被广泛接受,但免疫系统在银屑病中被触发的机制仍然是一个迷。

银屑病易感基因座PSORS1上的HLA-C*06:02(6p21.33)是银屑病主要风险等位基因。

最近的研究显示ADAMTS样蛋白5(ADAMTSL5)作为Vα3S1/Vβ13S1TCR的HLA-C*06:02呈递的黑素细胞自身抗原,可导致产生IL-17的T细胞的活化,从而引起银屑病发病。

本文将对这一新鉴定的银屑病的自身抗原作简要综述。

1.ADAMTSL5 的结构与功能1.1 ADAMTSL5结构含凝血酶敏感蛋白-1(TSP-1)基序的解聚蛋白样金属蛋白酶(a disintegrin-like and metalloproteinase with thrombospondin motifs,ADAMTS)超家族是一类整合于细胞外基质或游离于血浆中的基质金属蛋白酶亚家族,包括19种不同的ADAMTS蛋白[1]和至少7种ADAMTS-like(ADAMTSL)蛋白(ADAMTSL1-6和papilin)[2-3]。

ADAMTSL5是具有独特结构域的分泌型蛋白质,其包含N-末端TSR,富含半胱氨酸的模块,间隔基模块和C末端NTR模块,其通过富含脯氨酸的片段连接到间隔区(见图1)。

凝聚态物理实验第三章第二节

凝聚态物理实验第三章第二节

Related readings:
“More is Different – One more time” in “More is different” edited by Ong and Bhatt “A different universe – reinventing physics from the bottom DOWN” by Laughlin
Reference: The Nobel Prize lecture by Phillip W. Anderson
“Disordered electronic systems”, Patrick A. Lee, Rev. Mod. Phys. 57, 287 (1985) “Localization Yesterday, Today and Tomorrow” by Ramakrishnan in “More is Different”
Born 1923
According to ’s analysis of the influence of scientific research papers, Anderson is the most creative physicist in the world, followed by Steve Weinberg and Ed Witten.
extended state with mean free path l
localized state with localization length
Question:
• So far it seems the only role of disorder is to cause scattering of the Bloch waves without modifying the behavior of electrons qualitatively. What if we put in lots of impurities with strongly different impurity potential? Can the disorders cause fundamental change of the electronic

FULL_FORCE_RCEP

FULL_FORCE_RCEP

58OPINIONBy Hu YukunFULL FORCE RCEPith the Regional ComprehensiveEconomic Partnership (RCEP) entering into full force in early June, ASEAN countries will further benefit from enhanced economic integration in theAsia-Pacific region with better leverage to tackle unprecedented global challenges.Opportunities & Benefits from IntegrationOn June 2, the RCEP Agreement entered into force for thePhilippines, the last signatory to ratify the agreement. It marked full implementation of the mega free trade pact, under which more than 90 percent of trade in goods among approved member economies will gradually become tariff-free.ASEAN countries have already been benefiting from the regional economic integration since the ASEAN Free Trade Area agreement was signed in 1992, and under the new ASEAN + 1 framework, all 10 Southeast Asian countries have free trade agreements (FTAs) with Australia, China, Japan, New Zealand and South Korea, the five non-ASEAN signatories of the RCEP.The fully implemented RCEP pact seems to contain features of a typical FTA including removal of investment and trade barriers. But by addressing new emerging topics and balancing the differing economic positions of signatories, it could still provide more opportunities to continue boosting trade, enhancing supply chains, and improving the businessWHu Yukunenvironment of the region.For ASEAN countries, the RCEP will simplify rules and procedures of each FTA within a single arrangement and therefore reduce existing trade barriers. The so-called “noodle bowl effect,” coined for the counter-productive phenomenon of crisscrossing FTAs, will be avoided. A 2009 survey by Asian Development Bank showed that back then, only 45.1 percent of Chinese firms, 29 percent of Japanese firms, and 20.8 percent of South Korean firms used FTAs effectively, while 27 percent of responding firms across Asia believed multiple rules of origin (ROO) significantly added to business costs.Lower trade barriers and improved market access for goods and services make marketsmore attractive to companies from other participating countries as well as those from non-RCEP signatoriesinterested in entering a more integrated ASEAN market. The RCEP accounts for around a third of the world population, 30 percent of global GDP, over one fourth of global foreign direct investment (FDI) flows, and total trade of about US$10 trillion. Under a single consolidated rulebook, the potential of all 15 member states could be fully tapped with greater efficiency and convenient business as well as increased consistency in customs and trade.ASEAN countries are benefiting from a growing electronics sector, and thanks to well-established supply chains, they now have one of the highest intra-industry trade. With the RCEP set to reduce tariffs and other obstacles, Southeast Asiacould boost technology cooperation with advanced industrialized countries such as Japan, South Korea, Australia, and New Zealand, promoting the inclusion of local small and medium-sized enterprises into regional and global supply chains and helping them develop more competitive and value-added products. Through cooperation and competition, regional industries like telecommunications and agriculture will enjoygreater opportunities toflourish.As Chicago-based international lawfirm Baker McKenzie estimated, the RCEP will reduce or eliminate customs duties by around 92 percent,eliminate non-tariff measures, stipulate trade transparency and facilitation measures, and outline a detailed set of ROOs. All of these developments will enhance the business environment by increasing flexibility, protecting intellectual property, facilitating e-commerce, and regulating government procurement practices.This is why implementing the RCEP in a high-quality way is so important. Many officials and experts from ASEAN countries are confident that the framework provides a vision of shared prosperity and equitable distribution of economicComplete implementation of the RCEP is invigorating a more integrated ASEANThe RCEP will simplify rules and procedures of each FTA within a single arrangement and therefore reduce existing trade barriers.59OPINIONdevelopment across the Asia-Pacific region, which is exactly what Southeast Asia has long been seeking.Leverage Against UncertaintyASEAN countries are undoubtedly trade-dependent economies: Exports account for 70 percent of Thailand’s GDP, Singapore imports 90 percent of its food from over 170 countries and regions, RCEP members provide a market for 92 percent of products from the Philippines, and Malaysia’s trade is largely with other RCEPmembers. Such heavy dependence on world trade imposes potential risks on ASEAN countries while producing significant economic outcomes.Prior to the COVID-19 pandemic, business confidence and the global trade environment were already hampered by the US-China trade war and in a broad sense, growing tension between the two major powers. ASEAN countries were by no means coming out on top amid the major power rivalry. Vulnerable to external shocks, Singapore’s GDP contracted by 3.4 percent year-on-year in the second quarter of 2019. The same year, Malaysia,Singapore, and Vietnam were added to the watchlist of the U.S. Treasury Department for potential currency manipulation after the Trump administration labeled China a currency manipulator.Inward-looking policies induced by the pandemic and the Ukraine crisis further contribute to global uncertainty on economic integration. Sanctions and newly imposed trade barriers have been directly blocking cross-border business, and Southeast Asian countries have been directly impacted in sectors of tourism, food supply, and fuel prices. More importantly, a possible return to bloc politics is a fear for all ASEAN countries because it would shake the foundation of their economic engines.As the online news magazine The Diplomat pointed out, Southeast Asia needs to stay committed to multilateralism and continueworking towards economic reform and regional economic integration to bolster its resilience despite such economic uncertainty. In this sense, the RCEP could serve as leverage forASEAN countries to navigate various major global economies.The RCEP is now the world’slargest trade pact, covering some of the largest economies in the Asia-Pacific region. Accounting for around 30 percent of global trade in goods, its member states are presenting a powerful signal to the international community on maintainingeconomic globalization and regional integration. Since ASEAN alone is now the fifth largest economy and is on course to become the world’s largest single market by 2030, its participation in the RCEP has given the pact significant weight, which is in turn providing ASEAN countries an unprecedented opportunity to reshape the rules of global trade to better serve the region’s interests.Notably, ASEAN’s economiccooperation with China is rising to an even higher level, which could prove to be the greatest certainty among so much uncertainty in today’s global market. As the largest economy in the pact, China will certainly play a leading role in its implementation, especially its contribution to the global and regional supply chain, the vital part of the RCEP.Under the framework set by the pact, China’s integrated economic relations with ASEAN will not only stabilize the supply chain in the region, but also promote long-term industrial reform, support Southeast Asian tech development, infrastructure, and e-commerce, and improve the broader economic competitiveness of the region against possible cross-border risks.Over the past three decades, the thriving economy of theASEAN region has testified to the importance of open economy and regional integration. As the RCEP enters into full force, 10 ASEAN countries and other five member states are jointly tapping theireconomic potentials for even greater economic outcomes, and unleashing unprecedented power to contribute to global economic growth despite multiple challenges and concernsabout delocalization.Station staff prepare for the departure of the first train of a new international freight route connecting RCEP member countries and Europe through Shenyang, northeast China’s Liaoning Province, on March 18, 2023.(ZHAO GUIHUA)。

nucleophilicsubstitutionreactions

nucleophilicsubstitutionreactions
Solubility of nucleophile is a major problem in substitution reactions,
particularly in less polar aprotic solvents.
Crown ether is added to solvate the counter-cation which induce the solubility
of corresponding anionic nucleopon Correlation with Nucleophile
In SN2 reaction stronger the nucleophile faster would be the reaction. Strength of a nucleophile can be determined by the following general guidelines1. A nucleophile with negative charge is more powerful than its conjugate acid. Example: NH2─ >NH3, OH─ >H2O, 2. Nucleophilicity generally follows similar order as basicity Example: R3C ─ > R2N ─ > RO ─ > F ─
rate α [elec][nucl] In the presence of large excess of nucleophile, the kinetics tends to follow first
order even though the mechanism is bimolecular.

第四章π和σ电子的离域是失稳定的

第四章π和σ电子的离域是失稳定的

第四章π和σ电子的离域是失稳定的Chapter 4:π- and σ-Electron Delocalization Being Destabilizing(本次上传的是本章的第五部分)目录4. π-σ轨道作用是失稳定的 (1)5.σ-σ分子轨道作用 (7)5.1. FUL电子态的建立 (7)5.2. STO-3G是能量分解的合理基组 (13)5.3. LDSI电子态的建立 (14)5-4非成键σ-σ轨道作用是失稳定的 (20)5-5 σ-σ轨道作用是分子扭曲的驱动力 (22)6. 总结 (27)4. π-σ轨道作用是失稳定的E (θ) (h a r t r e e )Twist angle θοdegreeM o l e c u l a r E n e r g y E (θ) (h a r t r e e )Twist Angle θo(a)(b) (Figure 4-5-c)Figure 4-14. the molecular energies, E FUD(θ) , EDSI(θ) 和E (θ),of the FUD, DSI and ground state of NBA molecule,and their changes with the twist angle θ.在前面,已经讨论了π 电子的离域是失稳定的(图4-14),它是分子扭曲的驱动了. 在本节中,将讨论π-σ分子轨道作用的特性和它们对分子构象的影响. 在本章中,除了特别的说明,所有的电子态的计算都是以LFMO 为基组组的.以LFMO 为基,对分子构象做无条件的单点能运算, 可以得到一个构象的基态(G) (构象的基态当然也可以用标准的软件包算得. 但是,以LFMO 为基组做单点能计算,计算结果可以用于σ-π能量分解,也可以用于计算片断之间的电子转移能和交换能, 这将在能量分解的章节中详细地介绍和讨论). 基态也可以称为全离域的电子态. 分子构象的基态也可以认为是,FUD 电子态的π 和σ 分子轨道相互作用的结果. 因此,G 和FUD 态之间的分子能量(或电子总能量)差值,ΔE πσ(θ) = E (θ) - E FUD (θ),可以定义为π-σ轨道作用能(其中包含了n-π效应). 但是,这个轨道作用不仅仅是片断之间的作用,它还包括片断内的π-σ作用. 所以,在讨论π-σ之间的轨道作用对分子构象的影响时,应该分析片断之间和片断内的π-σ作用对总作用能ΔE πσ(θ)的贡献,和他们贡献的相对大小. 这些问题将在第五章《π-σ能量分解》中详细地讨论. 在FUD 和G 电子态中,σ和π轨道都离域在整个分子构架上,不存在片断分割数的差别. 所以, ΔE πσ(θ)的数值只与理论计算的方法和基组大小有关.Twist Angle θoΔEπσ(θ) (h a r t r e e )(a)(b)ΔE πσ(h a r t r e e )Twist angle θoFigure 4-15. The energy effect ∆E πσ(θ) associated with the π-σ interaction in NBA molecule, and its change with the twist angle θ.根据图4-15和表4-11, 在NBA分子中,π-σ轨道作用能∆Eπσ(θ)总是失稳定,这个结果与理论计算方法(DFT,RHF和MPn)和基组大小的选择无关. 在表4-11中,每一列的值都可以拟合成二次函数:∆Eπσ(θ) = a + bθ + cθ2(根据拟合方程,当θ = 0o时,∆Eπσ(θ) = a≠ 0, 这是拟合的误差. 所以在讨论时,可以不考虑拟合方程中截距a的因素). 根据图4-15-a和表4-11,在B3LYP水平下,拟合方程的104 b和106 c分别是:3.50592 和4.91272 (6-31Gd), 3.89744和5.71153 (6-311Gd), 4.4901和5.02887 (6-31G2d), 4.73066和6.00562 (6-311G2d). 这表明,d∆Eπσ/dθ = (b + 2c θ) > 0, d2∆Eπσ(θ)/dθ2 = 2c > 0, 而且基组越大,一阶导数d∆Eπσ(θ)/dθ和二阶导数d2∆Eπσ/dθ2就越大;根据图4-15-b,MPn法的一阶和二阶导数大于RHF法的, 在MP法中,MP2法的∆Eπσ(θ)最大,MP3的最小. 在RHF法中,基组越大,∆Eπσ(θ)的值越大. 结果,在NBA分子θ = 47o的构象中,∆Eπσ(θ)的大小顺序是:0.19195 (MP2/6-31Gd) > 0.16533 (MP4/6-31Gd) > 0.13774 (MP3/6-31Gd) > 0.061073 (RHF/6-31G) > 0.05914 (RHF/6-31Gd) > 0.057398 (RHF/4-31G) > 0.051980 (RHF/3-21G) > 0.02812 (RHF/STO-3G).因此,可以断言,π-σ电子离域是失稳定的,是NBA分子扭曲的阻力.Table 4-11.The molecular energy difference, ∆Eπσ(θ) = E(θ) – E FUD(θ), between the ground and FUD electron states for the rotational geometry of NBA molecule, and its change with the twist angle θ (energy unit in hartree) , RHF MP2 MP3 MP4(SDQ)B3LYPθo6-31Gd θ6-31Gd 6-311Gd 6-31G2d 6-311G2d0 0.0 0.0 0.0 0.0 0 0 0 0 017 0.00775 0.03656 0.02806 0.03018 170.00323 0.00329 0.00397 0.0039632 0.02853 0.11000 0.08185 0.09260 270.00867 0.00940 0.01058 0.0111047 0.05914 0.19195 0.13774 0.16533 370.0168 0.01878 0.02015 0.0218162 0.08966 0.25960 0.18245 0.22782 470.02677 0.03030 0.03158 0.0347877 0.11018 0.30016 0.20865 0.26628 570.03678 0.04176 0.04279 0.04755670.04528 0.05137 0.05217 0.05812770.05119 0.05799 0.05859 0.06532a -0.00376 -0.01178 -0.00825 -0.01014 -0.00204 -0.00246 -0.00247 -0.0028339.1 29.6 32.1 3.50592 3.89744 4.49014 4.73066B E+48.35196c E+69.14344 3.81598 -0.59839 6.69107 4.91272 5.71153 5.02887 6.00562 a, b, and c are the coefficients of the second order polynomial function ∆Eπσ(θ) = a + b θ + c θ2.为了探索π-σ作用对分子构象的影响(由于受计算软件的限制,我们早期的计算大多采用是RHF/STO-3G),计算了23个分子π-σ轨道作用能∆Eπ-σ(θ)(图4-16). 为便于阅读和比较,将图4-8-d并入到图4-16中. 比较4-16-c和4-16-d,在203个取代的二苯乙烯类化合物中,[∆Eπ-σ(90o)- ∆Eπ-σ(0o)] 约是0.06 hartree , [∆E V(90o) - ∆E V(0o)] 约是0.009 hartree,前者的值约是后者的6倍. 在表4-12 ~ 4-14中,列出了其中15个典型分子的π-σ分子轨道能∆Eπ-σ(θ). 所有的数据表明,在二苯乙烯类化合物中,∆Eπ-σ(θ) > 0.0始终成立,其一阶导数d[∆Eπ-σ(θ)]/dθ > 0 (表4-12和4-13),二阶导数d2[∆Eπ-σ(θ)]/dθ2 > 0. 所以,在二苯乙烯类分子中,π-σ作用也总是失稳定的,是分子扭曲的阻力.根据函数曲线的分布特征(图4-16),23个化合物可以分成两类. 第一类化合物含有−OH、−NO 2、−Cl 、−COOH 等取代基, 第二类是含−NH 2取代基. 在第一类化合物中, 根B3LYP/6-311G**优化构象的结构数据,在扭曲过程中,-OH 、-NO 2、-Cl 、-COOH 等取代基与母体苯环始终保持共平面. 它们的二次函数∆E π-σ(θ) = f (θ)的曲线几乎完全重叠, 对应的∆E π-σ(θ)当然非常接近(表4-12). 这表明, 当取代基与母体苯环共平面时,取代基对π-σ电子离域能的影响很小.ΔEπ−σ(h a r t r e e )Twist angle θoΔEπ−σ(h a r t r e e )Twist angle θo(a)(b)(c)Twist angle θοΔE π−σ (h a r t r e e )E v(h a r t r e e )Twist angle θο degree(d)6RFigure 4-16. (a) and (b) The energy effects ∆E π-σ(θ), associated with the π-σ interaction, for the substituted NBA molecules without NH 2 group and with a NH 2 group; (c) 23 Stilebene-like species; (d) the vertical resonance energies for 21 Stilebene-like species (Figure 4-8-d), and their changes with θ.但是表4-13-A 显示,−NH 2与它母体的两面β较大,约在10 - 28o 的范围内. 尤其是,随着分子构象的扭曲,β的角度也同时发生变化, 导致了分子5 (R 2 = −NH 2)、10 (R 2 = −NO 2,R 6 = −NH 2)、11 (R 2 = −NH 2,R 6 = −NO 2)、12 (R 2 = −NH 2,R 5 = −NO 2)、18 (R 3 = −NH 2,R 7 = −OH)和21 (R 2 = −NO 2,R 4 = −NH 2)的π-σ电子离域能∆E π-σ(θ)比其它分子更失稳定(图4-16-c 上部). 这5个化合物还可以分成两组,第一组包括化合物5、10、11和12,第二组由化合物18和21组成. 在第一组化合物中,随着扭角θ的增大,β角也单调增大;第二组化合物则相反,随着θ的增更大,β单调减小. 例如,在化合物5、11和12中,当扭角θ从0o 扭转到90o 时,取代基–NH 2 (R2)与其母体苯环的二面角β都从22o 左右增加到26o 左右. 与之对应的,它们的截距(a )、一阶导数(b + 2c θ)和二阶导数(2b )的值非常接近, 分别是:0.06512, 5.54485 x 10-4和 3.7434 x 10-6(5); 0.05637, 5.83322 x 10-4和4.39136 x 10-6(11); 0.05909, 5.87904 x 10-4和4.0416 x 10-6(12),都比其他化合物大. 所以,这三个化合物的函数曲线几乎重. 因此,在分子11、12、5中,-NH2和母体苯环之间的π-σ轨道失稳定作用比较大,对分子扭曲的阻力也比较大,从而导致较小的扭角θmin (分别为15.8o,-22.3o,29.2o). 在分子10中,当扭角θ从0o扭转到90o时,片断Q中的取代基–NH2 (R6)与其母体苯环的二面角β仅从19.8o增加到20.4o. 对应地,在图4-16-b中,分子10的函数曲线的一阶和二阶导数与其他无-NH2取代的分子非常接近(图4-16-c),因此它的θmin(45.12o)与其它无-NH2取代的分子的非常接近. 相反地,对于分子21,在扭角θ从0o扭转到67o过程中,取代基–NH2与桥键-N=C-的二面角β以及桥键-N=C-与片断Q中苯环的二面角θC分别从16.4o、25.6o降低到9.8o、22.8o。

p53对核仁应激诱导卵巢癌细胞凋亡的影响

p53对核仁应激诱导卵巢癌细胞凋亡的影响

p53对核仁应激诱导卵巢癌细胞凋亡的影响*陈松斌1,3, 徐可欣1, 潘炫豪3, 胡锴克3, 高维浩3, 李松岩1, 徐冶1,2△(1吉林医药学院肿瘤靶向治疗与转化医学实验室,吉林 吉林 132013;2吉林医药学院生殖功能损伤中药干预技术重点研究室,吉林 吉林 132013;3吉林医药学院临床医学院,吉林 吉林 132013)[摘要] 目的:探究p53对核仁应激诱导人卵巢癌SKOV3细胞凋亡的影响。

方法:体外培养SKOV3细胞,根据实验药物不同分成4个实验组:control 组、BMH -21(核仁应激诱导剂)组、pifithrin -α(PFT -α;p53抑制剂)+BMH -21组和RG7112(抑制p53降解并激活p53通路)+BMH -21组。

MTT 法检测细胞活力;免疫荧光染色检测细胞中p53蛋白定位和表达水平,以及BMH -21诱导的核仁应激的发生;Western blot 法检测蛋白表达水平;流式细胞术检测细胞凋亡率。

结果:MTT 结果显示,BMH -21组细胞活力比control 组显著下降(P <0.01),PFT -α+BMH -21组细胞活力与BMH -21组相比无显著变化(P >0.05),而RG7112+BMH -21组比BMH -21组显著下降(P <0.01);荧光显微镜观察发现,BMH -21组p53蛋白表达水平比control 组显著升高,PFT -α+BMH -21组p53蛋白表达水平比BMH -21组显著降低,而RG7112+BMH -21组比BMH -21组显著升高,且大部分入核堆积;Western blot 结果显示,BMH -21组p53蛋白及线粒体途径凋亡相关蛋白表达水平比control 组显著升高(P <0.01),PFT -α+BMH -21组p53蛋白表及线粒体途径凋亡相关蛋白表达水平比BMH -21组显著降低(P <0.05),而RG7112+BMH -21组比BMH -21组显著升高(P <0.01);流式细胞术结果显示,BMH -21组细胞凋亡率比control 组显著升高(P <0.01),PFT -α+BMH -21组细胞凋亡率与BMH -21组相比无显著变化(P >0.05),而RG7112+BMH -21组比BMH -21组显著升高(P <0.01)。

resonant 蟺-backbonding structure

resonant 蟺-backbonding structure

resonant 蟺-backbonding structureResonance refers to the delocalization of electrons in molecules or ions, resulting in the possibility of multiple different structures. This concept is crucial in understanding the stability and reactivity of organic and inorganic compounds. One example of resonance is the formation of a resonant π-backbonding structure.π-backbonding refers to the donation of electrons from a π-orbital of a donor atom to an empty d-orbital of an acceptor atom. This interaction is commonly observed in transition metal complexes where the transition metal acts as the acceptor atom. The presence of the π-backbonding structure can greatly influence the properties and reactivity of the compound.One classic example of a molecule exhibiting re sonance through π-backbonding is carbon monoxide (CO) interacting with transition metal complexes. Carbon monoxide is a strong π-acceptor ligand due to the presence of a low-lying π* antibonding orbital. The empty d-orbital of the metal atom acts as the donor orbital, forming a bonding interaction between the metal and CO.The general structure of a metal carbonyl complex can be represented as [M(CO)n]x, where M represents a metal atom, CO represents a carbon monoxide ligand, n represents the number of CO ligands, and x represents the charge on the complex. The CO ligands form a linear arrangement around the metal atom.One possible resonance structure of the metal carbonyl complex involves the donation of electrons from the filled d-orbital of the metal t o the π* antibonding orbital of CO, resulting in theformation of a π-backbonding interaction. As a result, a partial double bond character is formed between the metal and the carbon of CO. This resonance structure represents the delocalization of electrons and accounts for the stability and unique properties of metal carbonyl complexes.In addition to carbon monoxide, other ligands such as phosphines and alkyl isocyanides can also form π-backbonding structures with transition metal complexes. These ligand s possess π-acceptor character, making them suitable for interacting with metal d-orbitals and forming π-backbonding interactions.The presence of π-backbonding in transition metal complexes can affect various properties including bond lengths, bond strengths, and reactivity. For example, the formation of a π-backbonding structure can result in shorter metal-carbon bond lengths compared to similar compounds without π-backbonding. It can also influence the reactivity of the complex towards other ligands or substrates. In conclusion, resonance through π-backbonding is an important concept in understanding the stability and reactivity of transition metal complexes. The delocalization of electrons from π-acceptor ligands to the empty d-orbitals of the acceptor metal atom strengthens the metal-ligand bond and influences various properties. This concept is crucial in the field of coordination chemistry and the design of transition metal catalysts.。

π-σ轨道作用能分解法和限制π轨道作用的几何优化

π-σ轨道作用能分解法和限制π轨道作用的几何优化
cn2轨道作用能分解法和限制轨道作用的几何优化3虞忠衡33包鹏北京分子科学国家实验室中国科学院化学研究所分子动态稳态国家重点实验室北京100080摘要简要地介绍能量分解法的发展历史强调能量分解的特点是给量子化学的计算提供明确的化学意义介绍本实验室建立的能量分解法和限制轨道作用的几何优化法
第 19 卷 第 9 期 2007 年 9 月
化 学 进 展
PROGRESS IN CHEMISTRY
Vol . 19 No. 9 Sep . , 2007
π2σ轨道作用能分解法和限制π轨道作用的几何优化 3
虞忠衡 3 3 包 鹏
(北京分子科学国家实验室 中国科学院化学研究所 分子动态稳态国家重点实验室 北京 100080)
摘 要 简要地介绍能量分解法的发展历史 ,强调能量分解的特点是给量子化学的计算提供明确的化 学意义 ,介绍本实验室建立的能量分解法和限制轨道作用的几何优化法 。在这两个方法的基础上 ,论证了π2 电子离域是失稳定的 ;颠倒了经典有机结构理论中基本的因果关系 ———共轭效应和构象之间的因果关系 ;为 芳香能的计算提供了一个新的模型和新的方法 ,表明芳香能的计算不再需要参考分子 ;定量地区别静电作用 和电子离域在化学键形成中的作用 ;定量地讨论取代基效应和张力芳环的扭曲驱动力 。
Key words energy partition ; restrictions of orbital interactions ; geometry optimization ; electron delocalization ; aromaticity ; substituent effect ; cyclic strain
生于单键 C1 —N7 和 C8 —C9 的割断 。在 NBA 分子
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In this paper, we present a novel and efficient numerical technique to compute the two-particle localization length ξ2 directly from a microscopic model. This method allows us to obtain accurate results over a wide range of parameters for both bosons and fermions. Our main result is that ξ2 obeys the scaling relation ξ2/ξ1 = f (u/∆ξ), where ∆ξ is the single-particle level spacing of a wire of length ξ1. We conjecture, based on our numerical results, that the exact scaling function f is linear at the center of the band. While our results qualitatively confirm the prediction that a short-range interaction can lead to coherent propagation of the pair over distances much larger than the one-particle localization length, this scaling relation is inconsistent with the original prediction, Eq. (1). It is an important consequence of our results that the enhancement of ξ2 sets in for weaker interactions than previously predicted. We also derive and study a novel mapping of the problem to a bandedrandom-matrix model. A combination of scaling with this banded-random-matrix model suggests the possibility that the validity of our principal results extends to quasi-one-dimensional wires.
H = t {|n, m n+1, m| + |n, m n, m+1| + Vn + Vm) n, m| + U.
(2)
n,m
The random site energies Vn are drawn uniformly from the interval [−W/2, W/2]. The hopping matrix element t will be set to unity in the following. We parameterize the disorder by the one-particle localization length [1]
Shepelyansky considered the motion of two particles interacting by a short-range interaction in a random potential. Whenever the two particles are localized far apart compared to the one-particle localization length, the effect of the interaction is only exponentially small. However, an interesting effect occurs when the two particles are localized within about one one-particle localization length of each other. In this case, Shepelyansky constructed an approximate mapping of the problem to a banded-random-matrix model. Studying this model numerically, he predicted that independently of the statistics of the particles and the sign of the interaction there is the possibility of coherent propagation of the two particles over distances ξ2 much larger than the one-particle localization length ξ1 [4]. He found that
1
ξ1 = 105(t/W )2 in the absence of the interaction. For
bosons, we choose an on-site interaction with matrix elements n, m|U |n′, m′ = u δn,n′ δm,m′ δn,m, for spinless fermions a nearest-neighbor interaction with matrix elements n, m|U |n′, m′ = u[δn,m+1 + δn,m−1]δn,n′ δm,m′ . In the following our numerical method is described for
PACS numbers: 72.15.R, 71.30.
arXiv:cond-mat/9504016v2 7 Nov 1995
While much is known about the localization properties of one particle moving in a random potential [1], there are few secured results about localization in the presence of interactions between the particles [2]. In view of the complexity of the interplay of disorder and interaction, Dorokhov [3] and, very recently, Shepelyansky [4] approached this problem by studying a simple special case — two interacting particles in a random onedimensional potential — and predicted that the interaction can lead to a significant delocalization of the pair. A possible realization of this system are excitons in a disordered semiconductor [3]. Furthermore, understanding the localization properties of two particles in a random potential may lead to new insights into the role of interactions in the Anderson insulator.
Interaction-induced delocalization of two particles in a random potential: Scaling properties
Felix von Oppen, Tilo Wettig, and Jochen Mu¨ller Max-Planck-Institut fu¨r Kernphysik, 69117 Heidelberg, Germany
(November 7, 1995)
The localization length ξ2 for coherent propagation of two interacting particles in a random potential is studied using a novel and efficient numerical method. We find that the enhancement of ξ2 over the one-particle localization length ξ1 satisfies the scaling relation ξ2/ξ1 = f (u/∆ξ), where u is the interaction strength and ∆ξ the level spacing of a wire of length ξ1. The scaling function f is linear over the investigated parameter range. This implies that ξ2 increases faster with u than previously predicted. We also study a novel mapping of the problem to a banded-random-matrix model.
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