The millimetre variability of M81 -- Multi-epoch dual frequency mm-observations of the nucl
翻译 1
Mg-Al-Zn合金组织的晶粒尺在摩擦搅拌的依赖性弱搅拌处理的Y.N.王,a,b C.I.张,a C.J.李,a H.K.林a,c和黄祚芊a,*a材料科学与工程学院;纳米科学中心和纳米技术,国立中山大学圣大学,高雄804,台湾,中华民国b材料科学与工程学院,大连理工大学,大连116024,中国研究所c工研院南,工业技术研究院,台南县734,台湾,中华民国收到2006年4月25日;经修订的2006年5月18日;接受二零零六年六月七日可在网上二零零六年七月五日摘要在Mg-Zn-Al系合金热挤压加工通常表现出较强的粒度屈服应力的依赖。
然而,相同的摩擦搅拌处理的Mg-Zn-Al系合金的样品表现出弱得多的晶粒尺寸的依赖。
高施密特因子摩擦搅拌处理的样品在0.3左右,负责在的Hall-Petch关系的低参数。
关键词:镁合金;搅拌摩擦加工;纹理;晶粒尺寸的依赖镁合金已经吸引了运输车辆制造越来越大的兴趣,因为它们可以提供一个相当大的重量减少的结构。
然而,他们的延展性较差,由于在室温下的六边形结构可滑移系的数量有限,可能会限制其广泛应用。
另一方面,在镁合金的晶粒尺寸强化效率比以Al和其它合金高得多[1],这意味着晶粒细化镁合金是更有利。
大量的研究集中在镁合金的微结构上的修改已经进行,以提高和控制的机械性能[2-12]。
在镁合金中,存在基础和非基础滑移系之间的临界剪切应力(CROSS)有很大差异[13],这引起了严重的各向异性的机械性能。
其结果是,当变形镁合金具有强的晶体学织构在其微观结构,它们的机械性能显著由质地除了晶粒尺寸的影响[4-7,12,14]。
最近,研究搅拌摩擦加工(FSP)已经证明,有效的微观组织均匀化和细化可在镁基合金可以实现为严重的塑性变形和动态再结晶的结果。
已经发现该纹理具有强烈的不均匀分布沿着焊接工具的销柱表面基面的积累也带出,在搅拌区[8,15]。
有大量的报道[6,16-18]对晶粒尺寸和镁合金的基础上的Hall-Petch关系机械性能的关系之间的相关性。
人教版四年级语文下册《读课文 3 新奇的纳米技术(2018年1月安徽第1次印刷)》导读课_1
1 m
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红血球:200-300nm
细菌: 200-600nm
胃幽门 螺杆菌
病毒: 30-100nm
纳米粒子与病毒大小相当
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2、什么是纳米科技?
最早提出纳米尺度上科学和技术问题的 是美国著名物理学家、诺贝尔奖金获得者 理查德·费曼(Richard P Feynman)。
• 1959年费曼在一次题为《在底部还有很大空间》(“There is Plenty of Room at the Bottom. ”)著名的演讲中提出 “如果有 一天能按人的意志安排一个个原子和分子,将会产生什么样的奇 迹呢?”并预言,说人类可以用新型的微型化仪器制造出更小的 机器,最后人们可以按照自己的意愿从单个分子甚至单个原子开 始组装,制造出最小的人工机器来。可以说这些都是纳米技术的 最早的动意/梦想。
“纳”(nano)来自于希腊文,本意 是“矮子”或”侏儒”(dwarf)的意思。
纳米结构: 通常是指尺寸在100纳米以下(1-100nm) 的微小结构。
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一纳米有多小?
Human Hair
100 m
Take 1 slice
Human Hair
100 slices
Take 1 slice
1nm 1000 slices
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纳米科技的最终目的:是按人的意愿,操纵单个原子、分子, 构建纳米级的具有一定功能的器件或产品。
纳米科技研究的技术路线:可分为“自上而下”和“自下而上” 两种方式。
“自上而下(top-down)” —— 是指通过微加工或固态技术, 不断在尺寸上将人类创造的功能产品微型化 (传统技术);
传统的生产模式,是从大到小,从上到下的,比如造一个橱,要从粗木 到木材到成品;如造电脑,从半导体硅片,经过切、割、刻、蚀、渗杂 等许多步骤,变成电子元器件,这个过程,原材料浪费大、能耗大。
+Development of an intracavity-sumed multiple-wavelength Nd YAG laser for a rugged, solid-state sodi
Development of an intracavity-summedmultiple-wavelength Nd:YAG laser for a rugged,solid-state sodium lidar systemR.W.Farley and P.D.DaoA single-cavity solid-state laser that is resonant with sodium D2absorption is reported.Simultaneous1.06-and1.32-µm emission from a Q-switched Nd:YAG laser is summed with an intracavity type II KTPcrystal.A single-intracavity e´talon is sufficient to provide a time-averaged linewidth of1.7GHz at589nm.Key words:Sodium lidar.1.IntroductionSimultaneous lasing at multiple wavelengths with a common active medium has been reported by numer-ous investigators,using both solid-state and dye laser materials.1–3Bethea reported4summing the1.32-and1.06-µm emissions of a single Nd:YAG crystal that was simultaneously lasing at these wavelengths, producing589-nm output.His arrangement used overlapping,collinear cavities for the two wave-lengths,a common Q switch,and an extracavity KD*P crystal to mix the outputs.Jeys5subse-quently reported a summed Nd:YAG laser capable of resonantly exciting the atomic sodium D2transition. This laser used separate cavities and gain media for the production of synchronously mode-locked beams, which were externally summed.Yellow output was obtained from an intracavity-summed Nd:YAG by researchers at Lincoln labs,6but details were unpub-lished.In this paper we present results of frequency-summed emission obtained with a single cavity and gain medium.Development of a Nd:YAG laser with a single crystal,cavity,and Q switch that could provide a rugged,compact solid-state device capable of reso-nantly exciting atomic sodium appears promising.A reliable,easily aligned,transportable solid-state laser for sodium lidar is desired to facilitate investiga-tions of high-altitude atmospheric dynamics for im-proved neutral atmospheric modeling.Frequency mixing with a nonlinear optical crystal inside the oscillator exploits higher intracavity intensities and ensures a high degree of spatial overlap between the beams.The high damage threshold,acceptance angles,and nonlinear coefficients of KTP,which make it the material of choice for intracavity-doubled,Q-switched Nd:YAG lasers,suggested its use for this application.2.ExperimentAflash-lamp-pumped Nd:YAG crystal in a Q-switched oscillator that incorporates an intracavity KTP crys-tal produces589-nm output that is resonant with the absorption of atomic sodium.The oscillator is tuned by means of a thick13-mm2fused silica intracavity e´talon and through control of the cooling-water tem-perature,7yielding a stable,time-averaged linewidth of1.7GHz.Resonance with the sodium transition is established by observation of the optogalvanic output of a sodium hollow-cathode lamp.The0.635cm310.16cm Nd:YAG crystal is pumped by means of aflooded,close-coupled,diffuse-reflecting cavity provided by Big Sky Laser1Bozeman, Mont.2.The pump cavity is water cooled by a recircu-lating chiller.A heating coil spliced into the coolant return line shifts the summed emission into reso-nance with sodium.The optimum coolant tempera-ture is approximately50°C,although this value is not critical.The dual xenonflash lamps are simmered, and the main discharge is provided by a single-mesh pulse-forming network charged with an Analog Mod-ules controller.R.W.Farley is with PhotoMetrics Inc.,4Arrow Drive,Woburn, Massachusetts01801-2040.P.D.Dao is with Phillips Laboratory, OL-AA@GPIM,29Randolph Road,Bedford,Massachusetts 01731-3010.Received10June1994;revised manuscript received11October 1994.0003-6935@95@214269-05$06.00@0.r1995Optical Society of America.An 8mm 38mm 35mm 1height 3width 3length 2hydrothermally grown KTP crystal cut at u 578°,f 50°was provided by Philips Components 1Saugerties,N.Y.2.This device permits type II critical phase matching for 1.064µm 1o 2and 1.319µm 1e 2,producing 589nm 1o 2.A high effective nonlinear coefficient 8of d eff 53.5pm @V ,and relatively small 1.1°walkoff angle of the 1.319-µm beam from the phase-matching direction are expected.A single electro-optic Q switch suffices to hold off lasing during the flash-lamp pump pulse.The switch subsequently opens,providing low losses at all operat-ing sing is first obtained at 1.06µm,with subsequent emission at 1.32µm,in 25-ns and 45–65-ns FWHM pulses,respectively.An 18-ns pulse at 589nm is obtained during the overlap of the IR emissions.At the summed frequency,only forward-propagating emission is observed.In addition to 1.32µm,lasing is simultaneously obtained at the higher-gain 1.34-µm line.As the corresponding phase match angle is only 0.2°less,this line may also be summed with 1.06µm if the KTP crystal is slightly tilted,resulting in 592-nm output that peaks 230ns after the Q switch fires.This is 20ns earlier than the 589-nm output under identical pumping condi-tions.The optical configuration is shown in Fig.1.The rear mirror is a 18-m radius-of-curvature high reflec-tor at both 1.32and 1.06µm.The Q switch consists of a Glan–Taylor polarizer and a KD*P Pockels cell,which is maintained at the quarter-wave voltage for maximum hold off at 1.06µm.A high-voltage field-effect transistor switch grounds the Pockels cell in less than 10ns to open the Q switch.Without the high voltage,the laser operates in long-pulse mode at 1.34µm only,and rough alignment of the cavity isaccomplished by minimization of the buildup time for the first relaxation oscillation spike.Temporal per-formance at 1.3µm was monitored with a germanium photodiode,and a silicon photodiode was used for all other wavelengths.The KTP crystal is located as close to the plano 1r 5`2output coupler and,consequently,as close to the beam waist as possible.The z –x plane is tilted 45°from vertical so the fundamentals propagate as both o and e components,thus only half of each fundamental may contribute to the type IIsummedFig.1.Optical layout of the simultaneous multiple-wavelength,intracavity-summed Nd:YAG laser.The stable resonator oscillator is composed of a 18-m rear reflector and a flat output coupler that reflects IR and transmits the summed emission.A dichroic reduces feedback at 1.06µm to make oscillation at 1.32µm possible.The locations of both alternative wave plates are indicated in the schematic,although only one was used at a time,and only data collected with the quarter-wave plate are presented.HR,high reflector;PH,pinhole.Fig.2.Normalized,integrated optogalvanic response of the sodi-um–neon hollow-cathode lamp as a function of e ´talon tilt.The squares represent lamp output for a given e ´talon position averaged over 16laser shots.The solid curve represents the Doppler profile of 300°K sodium atoms.Despite considerable scatter,the data indicate resonance with and a laser linewidth comparable with or narrower than the Doppler-broadened absorption.output.To compensate for depolarization by KTP when it is used in this orientation,the optic axis of a quartz wave plate,l@4at both1.064and1.319µm,is subsequently aligned parallel to the plane of polariza-tion of the fundamentals.9This effectively inter-changes the o and e components so that the depolariza-tion by the KTP is precisely undone on the reverse pass through the crystal.A45°dichroic mirror rejects approximately90%of the1.06-µm beams and less than2%of the1.3-µm beams per pass.The cavity is terminated by a 1.32-µm high reflector, which reflects approximately35%of the 1.06-µm beam and serves as the589-nm output coupler.An aperture just before this mirror provides a degree of transverse-mode selection.The589-nm output crosses a45° 1.06-µm high reflector and an80-Åinterferencefilter before it is analyzed by the hollow-cathode lamp,Burleigh pulsed laser spectrum analyzer,or monitor e´talon.The final pulse energy after the interferencefilter1nomi-nal50%transmission2is30–80µJ,depending primar-ily on lamp energy and timing of the Q-switch open-ing.To suppress feedback at1.06µm and unintentional e´talon effects,all intracavity surfaces were antireflec-tion coated for both1.06and1.32µm,except forthe Fig.3.Spectral analysis of laser performance for each of the wavelengths produced in this study,measured with a200-MHz resolution Fabry–Perot e´talon.The589-nm and592-nm signals were produced intracavity with KTP,and the others were externally doubled with KD*P.For each wavelength,a single-shot and a smoother200-shot average are superimposed.e´talon,mirrors,and Pockels cell,which is coated for only1.06µm.Once the operating temperature is attained,the frequency of the summed output is adjusted by one’s angle tuning3-mm fused silica e´talon with coatings that provide low reflectivity at 1.3µm and70%reflectivity at1.06µm.To facilitate analysis,an external type II KD*P crystal doubled each IR wavelength when it was tuned to the appropri-ate angle,producing more readily analyzed532-, 660-,and669-nm outputs.The doubler accepted reflections off the dichroic,which attenuated the 1.06-µm transition.3.ResultsAlthough the laser may typically be tuned several gigahertz by one’s tilting the e´talon,this process significantly varies walkoff losses,effective gain,and consequently the buildup time for the1.06-µm oscilla-tion.No such temporal variation is observed for the 1.3-µm emissions,although both doubled outputs at 660and669nm are spectrally shifted with the e´talon position.Thus tilting the e´talon not only tunes the emissions but also varies the relative timing between them.Ultimately no overlap between1.06and1.32µm is obtained,and no summed output is observed. This result may be compensated by slight variation of theflash-lamp energy162%2or cavity losses at1.06µm.The latter approach may be readily effected by the use of a wave plate that is l@1at1.32µm and l@2 at1.06µm and placed between the Nd:YAG and KTP crystals.When the polarization of the1.06-µm fun-damental is rotated nearly90°,the KTP crystal can be mounted normally,instead of at45°,as is required for type II interactions when the input polarizations are parallel.This arrangement allows the full intensi-ties of the fundamentals to contribute to the summed output.It also eliminates depolarization losses,and thus the need for the quarter-wave plate compensator. Slight rotation of the half-wave plate has a negligible effect on the1.32-µm radiation but greatly affects losses at 1.06µm,readily compensating for those induced when the e´talon is tilted.Although slightly lower lamp energies are required,no other significant differences were detected as a result of which method was used.The data presented in this paper were obtained with the quarter-wave plate compensator, with the KTP crystal mounted at45°.The optogalvanic response of a hollow-cathode lamp demonstrates resonance with the sodium D2 absorption.A plot of lamp output versus e´talon tilt is presented in Fig.2.The squares in Fig.2repre-sent normalized integrated lamp output averaged over16laser shots for each e´talon angle.The theoretical curve corresponds to the Doppler line-width for300°K sodium,which is considered a lower limit for the actual temperature of sodium atoms in the lamp.For each e´talon tilt,theflash-lamp energy was adjusted for maximum output at589nm.The data show reasonable agreement with the Doppler profile,which suggests only a relatively small contri-bution from the laser linewidth.Errors are primar-ily shot-to-shot intensityfluctuations at589nm and uncertainties in e´talon position that are due to the limited resolution of its mount.A more accurate relative measurement of laser spectral performance is provided by a wavemeter 1Burleigh PLSA-35002that incorporates230-GHz Fizeau and10-GHz free-spectral-range Fabry–Perot e´talons with enhanced silver coatings and CCD detec-tors.The latter e´talon provides200-MHz resolution, which is adequate for all laser outputs reported in this study.Wavelengths were computed with the Fizeau wedge,and linewidths and line shapes were mea-sured from the Fabry–Perot fringe patterns.Results of spectral analyses are presented in Fig.3for both single-shot and200-shot averages.The intracavity e´talon has a35-GHz free-spectral range and a reflectivefinesse of9at1.06µm,provid-ing a single-pass resolution of3.9GHz.This results in a360-MHz linewidth at532nm.By contrast the e´talon has a reflectivefinesse less than1at1.3µm. Compared with532nm,the660-nm and669-nm emissions are successively broader,resulting in summed linewidths in the same order,with589-nm output narrower than the output at592nm.Clearly the broad 1.3-µm emission poses the more severe limitation on the spectral performance of this system. Also,despite the antireflective coatings,strong modu-lation of the1.3-µm emissions is observed because of slight reflections from the ends of the Nd:YAG rod. 4.ConclusionsThe wavemeter and hollow-cathode lamp analyses demonstrate resonance with and a linewidth compa-rable with the Doppler-broadened sodium absorption. The laser provides a compact,stable,solid-state source for resonant excitation of sodium,which is tuned by one knob.The only other required adjustments are optimization of summing crystal orientation andflash-lamp power.Currently the energy obtained at589 nm represents only0.1–0.2%of the total output. The low summing efficiency could be greatly en-hanced by improvement of temporal overlap between the summed transitions and by better spectral control of the1.3-µm emission.For this laser to be part of a practical sodium lidar system,kilohertz repetition rates or at least an order of magnitude higher pulse energies are required.The summed linewidth is comparable with and limited by the1.32-µm emission.Seeding this tran-sition would greatly reduce the589-nm linewidth, discriminate against1.338-µm emission,and reduce the Q-switch buildup time for better overlap with 1.06-µm emission.Addition of a thin e´talon instead may also significantly improve performance,with much less additional bulk and complexity.The sim-plest modification,the use of mirrors with lower reflectivity at1.338µm,could suppress this parasitic transition and increase efficiency but would provide no improvement in linewidth at589nm.Varying the Pockels cell voltage to open the Q switch more slowly may also improve spectral quality and reduce intensityfluctuations of the589-nm emis-sion.Although the16-shot averages used for spec-tral analysis were relatively stable,shot-to-shot en-ergyfluctuations of630%were typical.This largely results from jitter in the timing of the 1.06-µm emission,which affects the overlap between the funda-mentals and thus the summed intensity.Both the temporal profile and intensity at1.3µm are very stable,essentially insensitive to e´talon position,and only slowly varying withflash-lamp energy. Although more sophisticated control of the Q switch requires only modest additional electronic complex-ity,it may prove a dubious modification unless addi-tional discrimination is provided for the1.338-µm transition.Although the mirrors are highly reflec-tive for1.32µm,the Q switch favors longer wave-lengths.As the Pockels cell is maintained at the quarter-wave potential for1.06µm,discrimination is highest for this wavelength and progressively less for 1.32and 1.34µm.Were the Pockels cell to be opened slowly,this could further favor the higher-gain,longer-wavelength emission,enhancing592-nm instead of589-nm production.This work was supported under U.S.Air Force contract F19628-92-C-0160.References1.R. A.Morgan, F. A.Hopf,and N.Peyghambarian,‘‘Dual-frequency Nd:YAG laser for the study and application of nonlin-ear optical crystals,’’Opt.Eng.26,1240–1244119872.2.H.Y.Shen,Y.P.Zhou,W.X.Lin,Z.D.Zeng,R.R.Zeng,G.F.Yu,C.H.Huang,A.D.Jiang,S.Q.Jia,and D.Z.Shen,‘‘Secondharmonic generation and sum frequency mixing of dual wave-length Nd:YALO3laser influx grown KTiOPO4crystal,’’IEEE J.Quantum Electron.28,48–51119922.3.Y.Saito,T.Teramura,A.Nomura,and T.Kano,‘‘Simulta-neously tunable three-wavelength dye laser,’’Appl.Opt.24, 2477–2478119852.4. C.G.Bethea,‘‘Megawatt power at1.318µin Nd31:YAG andsimultaneous oscillation at both1.06and1.318µ,’’IEEE J.Quantum Electron.QE-9,254119732.5.T.H.Jeys,‘‘Development of a mesospheric sodium laser beaconfor atmospheric adaptive optics,’’Lincoln Lab.J.4,133–149 119912.6.P.Schultz,MIT Lincoln Laboratories,Lexington,Mass.021731personal communication,19932.7.J.Marling,‘‘1.05–1.44µm tunability and performance of the cwNd31:YAG laser,’’IEEE J.Quantum Electron.QE-14,56–62 119782.8.H.Vanherzeele and J.D.Bierlein,‘‘Magnitude of the nonlinear-optical coefficients of KTiOPO4,’’Opt.Lett.17,982–984119922.9.K.C.Liu and M.G.Cohen,‘‘High-power Nd:YAG laser at532nm using intracavity type11KTP,’’in Digest of Conference on Lasers and Electro-Optics1Optical Society of America,Washing-ton,D.C.,19862,p.110.。
(3)--2010级细胞生物学期末试卷-参考答案-20130116
中山大学生科院细胞生物学期末试卷(A卷)(2010级生物科学、生物技术、生技应用、生态学、逸仙班等共280人)任课教师:王金发王宏斌考试时间:2013年1月16日上午9:30~11:30考场:东校区E101,E201课室参考答案一、填空题(每空2分,共10分)1、在M期可能有一种是染色体压缩包装的“打包工”的存在。
2、透明、细胞数量少,便于绘制细胞谱系图。
3、条件致死突变,在非致死条件下得到的突变体只影响分裂而不致死。
4、志愿者。
5、“心中有数”。
6、没有提升到假说及理论高度。
7、Truth is not a citadel of certainty to be defended against error; it is a shady spotwhere one eats lunch before tramping on.8、“天网恢恢疏而不漏”。
9、排球队中的一流二传手!10、“疏导有方”。
二、判断以下各题是否正确, 若正确, 用T表示, 不正确用F表示,并给出简要说明,每题1分,共5分1. Ans:True.The barriers formed by tight junction proteins restrict the flow ofmolecules between cells and the diffusion of proteins(and lipids)from the apical to the basolateral domain and vice versa.2. Ans:False.During transcytosis,vesicles that form from either the apical or basolateralsurface first fuse with early endosomes, then move to recycling endosomes,where they are sorted into transport vesicles bound for the opposite surface.3. Ans: True. All the core histones are rich in lysine and arginine, whichhave basic—positively charged—side chains that can neutralize thenegatively charged DNA backbone.4. Ans: True. When ATP in actin filaments (or GTP in microtubules) is hydrolyzed,much of the free energy released by cleavage of the high-energy bond is storedin the polymer lattice, making the free energy of the ADP-containing polymerhigher than that of the ATP-containing polymer. This shifts the equilibriumtoward depolymerization so that ADP-containing actin filaments disassemblemore readily than ATP-containing actin filaments.5. Ans: False. Although cyclin–Cdk complexes are indeed regulated byphosphorylation and dephosphorylation, they can also be regulatedby the binding of Cdk inhibitor (CKI) proteins. Moreover, the ratesof synthesis and proteolysis of the cyclin subunits are extremelyimportant for regulating Cdk activity.三、选择题(请将所选答案字母填入括号,简要说明选择理由,每题1分,共5 分)1、答:B2、答: B3、答: A4、答:B5、答:C四、简答题(每题5 分,共25分)1.Ans: The specificity for both the transport pathway and the transported cargo come notfrom the clathrin coat but from the adaptor proteins that link the clathrin to the transmembrane receptors for specific cargo proteins.The several varieties of adaptor proteins allow different cargo receptors,hence different cargo proteins,to be transported along specific transport pathways.Incidentally, humans are different from most other organisms in that they have two heavy-chain genes.Like other mammals,they also have two lightchain genes. In addition,in the neurons of mammals the light-chain transcripts are alternativelyspliced.Thus,there exists the potential in humans for additional complexity ofclathrin coats;the functional consequences of this potential variability are notclear.2.Ans: The building blocks—soluble subunits—of the three types of filaments are the basis for their polarity differences. The building blocks for actin filamentsB (an actinmonomer) and microtubules (ab-tubulin) have polarity—distinct ends—and thusform a polymer with distinct ends when they are linked together. By contrast, thebuilding block of intermediate filaments is a symmetric tetramer with identical ends.Thus, when these subunits are linked together, the ends of the resulting filament are also identical.3.Ans: Soluble ER proteins that are destined to reside in other membrane organelles or to be secreted are bound by transmembrane cargo receptors.The cytosolic domains ofthese cargo receptors bind to the COPII coats on the vesicles that form on the ERmembrane, incorporating the cargo receptors, along with their cargo, intoCOPII-coated vesicles.4.Ans: Because programmed cell death occurs on a large scale in both developing and adult tissues, it is important that it does not trigger the alarm reactions normallyassociated with cell injury. In tissue injury, for example, signals are released thatcan cause a destructive inflammatory reaction. Moreover, the release of intracellularcontents could elicit an immune response against molecules that are normally notencountered by the immune system. In normal development, such reactions would be self-defeating, even dangerous, if they occurred in response to programmed cell death.5、答:不能,如①核定位信号是永久性信号,需要反复使用,而通过ER的信号肽通常是临时性信号,如果核定位信号被切除,不利于有丝分裂后核的重建。
果蝇体内SOD和MDA随增龄变化及其与寿命的关系_张欣文
果蝇体内SOD和MDA随增龄变化及其与寿命的关系上海铁道大学医学院预防医学教研室(200070)张欣文徐思红厉曙光提要每日观察记录果蝇生存数和死亡数;将不同天龄雌雄果蝇分别制成匀浆,测定匀浆中SOD活力和M DA含量。
结果显示果蝇体内SO D活力在40、50天龄以前是随增龄而上升,进入老龄后开始下降;M DA含量随增龄而增加;各天龄雄蝇的SOD均高于雌蝇(P<0101);40天龄后雄蝇的存活率明显低于雌蝇(P<0.05或P<0101)。
表明果蝇体内SOD活力与M DA 含量的平衡失调可能是引起衰老和死亡的原因之一。
关键词黑腹果蝇SO D M DA寿命Eff ect of Age-dependent Variation of Superoxide Dismutse Activ-ity and Malonaldehyde Contents on Lif e-span in Drosophila Zhang X inwen,et al.D ep t.of Pr eventive M edicine,M edical College,Shanghai T iedao Univer sity(Shanghai200070) T o study the age-dependent var iation of sup er ox ide dis-mutse(SOD)activity and malonaldehyde(MDA)contents in Dr osop hila melanogaster and the corr elationship between the v ar i-ation and lif e-sp an.T he number s of liv ing and died f lies w er e recor ded daily.Flies of diff er ent ages(days)w er e homogenated sep arately.T he ex amining the SOD activ ity and M DA contents in the homogenates have been made.A ges of D rosop hila being y ounger than40、50days,the S OD activ ity increased with ad-vancing age,w hile that decr eased in aged Dr osop hila.T he levels of M DA incr eased signif icantly w ith a ging.T he S OD activity of male f lies is signif icant higher than that of f emale f lies in ev-ery group.T he p er cent sur v iv al of male f lies is signif icant low er than that of f emale f lies af ter40days.T he unbalance betw een SOD and MD A could be one of the f actor s causing aging and death.Key words Dr osophila melanogaster Superox ide dismutse Malonaldebyde Life-span果蝇虽属昆虫,但其许多代谢途径、生理学功能和发育阶段同哺乳动物基本相似,故常被用于衰老实验研究。
微卫星单碱基的英语
微卫星单碱基的英语英文回答:Microsatellites are short, repetitive sequences of DNA that are composed of a single nucleotide, such as A, C, G, or T. These sequences are found throughout the genome and are often located in non-coding regions. Microsatellites are highly polymorphic, meaning that they vary in length between individuals. This polymorphism makes them usefulfor a variety of genetic applications, such as population genetics, forensic science, and paternity testing.The most common type of microsatellite is the dinucleotide repeat, which consists of two nucleotides repeated in tandem. Dinucleotide repeats are typically named after the two nucleotides that they are composed of, such as AC, AG, or AT. Other types of microsatellites include trinucleotide repeats, tetranucleotide repeats, and pentanucleotide repeats.Microsatellites are thought to arise through a process called replication slippage. This process occurs when DNA polymerase pauses during replication and repeats the same nucleotide several times. Microsatellites can also be created or lengthened through a process called unequal crossing-over. This process occurs when two homologous chromosomes misalign during meiosis and exchange genetic material.Microsatellites are highly polymorphic because they are prone to mutations. These mutations can occur through a variety of mechanisms, including replication slippage, unequal crossing-over, and gene conversion. Microsatellite mutations can have a number of different effects, including changes in gene expression, changes in protein structure, and changes in disease susceptibility.Microsatellites are a valuable tool for a variety of genetic applications. They are used in population genetics to study the genetic diversity of populations and to track the flow of genes between populations. Microsatellites are also used in forensic science to identify individuals andto link suspects to crime scenes. Microsatellites are also used in paternity testing to determine the biologicalfather of a child.中文回答:微卫星是长度较短的重复性 DNA 序列,由单个核苷酸组成,如A、C、G 或 T。
常用酿酒酵母菌株基因型
常⽤酿酒酵母菌株基因型Commonly used strainsinformation include:used lab strainsidentity between common lab strainsS288CGenotype:MATαSUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.The S288C genome was recently resequenced at the Sanger Institute.References:Mortimer and Johnston (1986) Genetics 113:35-43.BY4743Genotype:MAT a/αhis3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite frequency. See Brachmann et al. reference for details.References:Brachmann et al. (1998) Yeast 14:115-32.FY4Genotype:MAT aNotes: Derived from S288C.References:Winston et al. (1995) Yeast 11:53-55.FY1679Genotype:MAT a/αura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GALNotes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Winston et al. (1995) Yeast 11:53-55.AB972Genotype:MATα X2180-1B trp10 [rho 0]Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.References:Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.A364AGenotype:MAT a ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONotes: Used in the systematic sequencing project, the sequence stored in SGD.References:Hartwell (1967) J. Bacteriol. 93:1662-1670.XJ24-24aGenotype:MAT a ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2Notes: Derived from, but not isogenic to, S288CReferences:Strathern et al. (1979) Cell 18:309-319DC5Genotype:MAT a leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References:Broach et al. (1979) Gene 8:121-133X2180-1AGenotype:MAT a SUC2 mal mel gal2 CUP1Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180YNN216Genotype:MAT a/αura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochreNotes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin). References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH499Genotype:MAT a ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Notethat trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH500Genotype:MATαura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.YPH501Genotype:MAT a/MATαura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochretrp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1Notes:a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References:Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sigma 1278BNotes: Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology.Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found here. SK1 Genotype:MAT a/α HO gal2 cup S can1R BIONotes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative.The SK1 genome was sequenced at the Sanger Institute.References:Kane SM and Roth J. (1974) Bacteriol. 118: 8-14CEN.PK (aka CEN.PK2)Genotype:MAT a/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8CSUC2/SUC2Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delaysReferences:van Dijken et al. (2000) Enzyme Microb Technol 26:706-714W303Genotype:MAT a/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variantof MIP1 which increases petite frequency.The W303 genome was sequenced at the Sanger Institute.References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).bud4 info: Voth et al. (2005) Eukaryotic Cell, 4:1018-28.rad5-535 info: Fan et al. (1996) Genetics 142:749W303-1AGenotype:MAT a {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.W303-1BGenotype:MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}References: W303 constructed by Rodney Rothstein (see detailed notes from RR and Stephan Bartsch).W303-K6001Genotype:MAT a; {ade2-1, trp1-1, can1-100, leu2-3,112, his3-11,15, GAL, psi+, ho::HO::CDC6 (at HO), cdc6::hisG,ura3::URA3 GAL-ubiR-CDC6 (at URA3)}References: K6001 was developed by Bobola et al in Kim Nasmyth's lab (PMID: 8625408), and has become a common model in yeast aging research (PMID: 15489200). Its genome has been sequenced by Timmermann et al (PMID: 20729566) D273-10BGenotype:MATαmalNotes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.References:Sherman, F. (1963) Genetics 48:375-385.FL100Genotype:MAT aReferences:Lacroute, F. (1968) J. Bacteriol. 95:824-832.Sources: ATCC: 28383SEY6210/SEY6211Genotype:MAT a/MATαleu2-3,112/leu2-3,112 ura3-52/ura3-52 his3-Δ200/his3-Δ200 trp1-Δ901/trp1-Δ901ade2/ADE2 suc2-Δ9/suc2-Δ9 GAL/GAL LYS2/lys2-801Notes: SEY6210/SEY6211, also known as SEY6210.5, was constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers, good growth properties and good sporulation.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48SEY6210Genotype:MATαleu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 suc2-Δ9 lys2-801; GALNotes: SEY6210 is a MATalpha haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48SEY6211Genotype:MAT a leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 ade2-101 suc2-Δ9; GALNotes: SEY6211 is a MATa haploid constructed by Scott Emr and has been used in studies of autophagy, protein sorting etc. It is the product of crossing with strains from 5 different labs (Gerry Fink, Ron Davis, David Botstein, Fred Sherman, Randy Schekman). It has several selectable markers and good growth properties.References:Robinson et al. (1988) Mol Cell Biol 8(11):4936-48JK9-3dThere are a, alpha and a/alpha diploids of JK9-3d with the following genotypes:Genotypes: JK9-3da MAT a leu2-3,112 ura3-52 rme1 trp1 his4JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locusJK9-3da/α is homozygous for all markers except mating typeNotes: JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.88(5):1948-52RM11-1aGenotype:MAT a leu2Δ ura3Δ ho::KanNotes: RM11-1a is a haploid derivative of Bb32(3), a natural isolate collected by Robert Mortimer from a California vineyard, as in Mortimer et al., 1994. It has high spore viability (80–90%) and has been extensively characterized phenotypically under a wide range of conditions. It has a significantly longer life span than typical lab yeast strains and accumulates age-associated abnormalities at a lower rate. It displays approximately 0.5–1% sequence divergence relative to S288c. More information is available at the Broad Institute website.References:Brem et al. (2002) Science 296(5568):752-5Y55Genotype:MAT a /MAT alpha HO/HONotes: Y55 is a prototrophic, homothallic diploid strain that was originally isolated by Dennis Winge. Many auxotrophic mutant derivatives have been created by John McCusker by using ethidium bromide treatment to eliminate。
薛定谔—麦克斯韦尔方程径向解的存在性和多重性(英文)
In 1887, the German physicist Erwin Schrödinger proposed a radial solution to the Maxwell-Schrödinger equation. This equation describes the behavior of an electron in an atom and is used to calculate its energy levels. The radial solution was found to be valid for all values of angular momentum quantum number l, which means that it can describe any type of atomic orbital.The existence and multiplicity of this radial solution has been studied extensively since then. It has been shown that there are infinitely many solutions for each value of l, with each one corresponding to a different energy level. Furthermore, these solutions can be divided into two categories: bound states and scattering states. Bound states have negative energies and correspond to electrons that are trapped within the atom; scattering states have positive energies and correspond to electrons that escape from the atom after being excited by external radiation or collisions with other particles.The existence and multiplicity of these solutions is important because they provide insight into how atoms interact with their environment through electromagnetic radiation or collisions with other particles. They also help us understand why certain elements form molecules when combined together, as well as why some elements remain stable while others decay over time due to radioactive processes such as alpha decay or beta decay.。
异构催化剂生产生物柴油1111
Heterogeneous Catalysts for Biodiesel ProductionMartino Di Serio,†Riccardo Tesser,†Lu Pengmei,‡and Elio Santacesaria*,†Dipartimento di Chimica,Uni V ersitàdi Napoli “Federico II”,V ia Cintia 80126Napoli,Italy,andGuangzhou Institute of Energy Con V ersion,Academy of Science,ChinaRecei V ed May 17,2007.Re V ised Manuscript Recei V ed September 7,2007The production of biodiesel is greatly increasing due to its enviromental benefits.However,production costs are still rather high,compared to petroleum-based diesel fuel.The introduction of a solid heterogeneous catalyst in biodiesel production could reduce its price,becoming competitive with diesel also from a financial point of view.Therefore,great research efforts have been underway recently to find the right catalysts.This paper will be concerned with reviewing acid and basic heterogeneous catalyst performances for biodiesel production,examining both scientific and patent literature.IntroductionNowadays,biodiesel (a mixture of fatty acid methyl esthers,FAMEs)has become very attractive as a biofuel because of its environmental benefits s it has less air pollutants per net energy than diesel and is nontoxic and biodegradable s and because it is produced from renewable sources with high energetic efficiency:biodiesel yields from an estimated 90%1to 40%2more energy than the energy invested in producing it.Most biodiesel is produced today by the transesterification of triglycerides of refined/edible type oils using methanol and an alkaline catalyst (NaOH,NaOMe):3–5The reaction isnormally performed at 60–80°C.The glycerol and FAME are separated by settling after catalyst neutralization.The crude glycerol and biodiesel obtained are then purified.However,production costs are still rather high,compared to petroleum-based diesel fuel.3There are two main factors that affect the cost of biodiesel:the cost of raw materials and the cost of processing.3Processing costs could be reduced through simplified opera-tions and eliminating waste streams.6–9A solution to thisproblem could be transesterification in supercritical methanol without using any catalyst.6,7As a matter of fact,in this case,the reaction is very fast (less than 5min)and the absence of catalyst decreases downstream purification costs.6,7Even if some production plants use this technology in Europe,6the reaction requires very high temperatures (350–400°C)and pressures (100–250bar)and thus high capital costs.6,7The use of heterogeneous catalysts could be an attractive solution.8,9As a matter of fact,heterogeneous catalysts can be separated more easily from reaction products and the reaction conditions could be less drastic than the methanol supercritical process.In 2006a 160000t/y commercial plant started up using a heterogeneous catalyst.8The plant is based on the Hesterfip-H technology developed by the Institute Français du Petrole (IFP).8,9The catalyst employed in the Hesterfip-H technology is a mixed oxide of zinc and aluminum.9The Hesterfip-H technology operates at 200–250°C but does not require catalyst recovery and aqueous biodiesel treatment steps:the purification steps of products are therefore much more simplified and very high yields of methyl esters s close to theoretical values s are obtained.6,9Glycerol is directly produced with high purity levels (at least 98%)and is free from any salt contaminants.9This aspect is very important from the economical point of view because it reduces the cost of obtaining high-grade glycerol,thus increasing the profitability of the process.Moreover,in order to lower the costs and make biodiesel competitive with petroleum-based diesel,less-expensive feed-stocks such as waste fats or nonedible type oils,could be used.3–7,10However homogeneous alkaline catalysts in the transesterification of such fats and oils cannot directly be used due to the presence of large amounts of free fatty acids (FFAs);3–5for the use of these catalysts,the FFA concentration should be less than 0.5%(w/w)to avoid the formation of high*Corresponding author.Fax:0039081674026.E-mail:elio.santacesaria@unina.it.†Universitàdi Napoli “Federico II”.‡Academy of Science.(1)Hill,J.;Nelson,E.;Tilman,D.;Polasky,S.;Tiffany,D.Proc.Natl.Acad.Sci.2006,103,11206–11210.(2)Wesseler,J.Energy Policy 2007,35,1414–1416.(3)Ma,F.;Hanna,M.A.Bioresour.Technol.1999,70,1–15.(4)Pinto,A.C.;Guarieiro,L.L.N.;Rezende,M.J.C.;Ribeiro,N.M.;Torres,E.A.;Lopes,W.A.;de P.Pereira,P.A.;de Andrade,J.B.J.Braz.Chem.Soc.2005,16,1313–1330.(5)Lotero,E.;Liu,Y.;Lopez,D.E.;Suwannakaran,K.;Bruce,D.A.;Goodwin,J.G.,Jr.Ind.Eng.Chem.Res.2005,44,5353–5363.(6)Huber,G.W.;Iborra,S.;Corma,A.Chem.Re V .2006,106,4044–4098.(7)Demirbas,A.Prog.Energy Combust.Sci.2007,33,1–18.(8)Dupraz,C.BIO-Energy -Prospect for India-France Partnership -18th April 2007-New Delhi,/events/4001/Mr_Dupraz.pdf (accessed Aug 2007).(9)Bournay,L.;Casanave,D.;Delfort,B.;Hillion,G.;Chodorge,J.A.Catal.Today 2005,106,190–192.(10)Kulkarni,M.G.;Dalai,A.K.Ind.Eng.Chem.Res.2006,45,2901–2913.Energy &Fuels 2008,22,207–21720710.1021/ef700250g CCC:$40.75 2008American Chemical SocietyPublished on Web 12/04/2007soap concentrations as a consequence of the reaction of FFAs with the basic catalyst:R-COOH +NaOH f R-COOHNa +H 2OThe soap causes downstream processing problems in product separation because of emulsion formation.3,6Several methods have been proposed to solve these prob-lems,11but the most useful seem to be the following:5,10,12–15(a)use of enzymes.A Lypase enzyme catalyzes both transesterification of triglycerides and esterification of FFAR-COOH +MeOH a R-COOMe +H 2Oin one step.9,11–13(b)use of acid catalysts.Acid catalyst can also promote esterification and transesterification.5,10,15(c)pre-esterification method.FFAs are first esterified to FAMEs using an acid catalyst,and then,transesterification is performed,as usual,by using an alkaline catalyst.5,10Enzyme-based transesterification is carried out at moderate temperatures with high yields,but this method cannot be used in industry today due to high enzyme costs,and the problems related to its deactivation caused by feed impurities.10,12–14The enzyme can be immobilized on a support to obtain a heterogeous catalyst,but again,the use will only be possible if the enzyme costs are reduced s as in the case of enzyme use in detergents,dairy products,textile,and leather processing.12However,the use of enzymes is not explored in this review which will be devoted to the use of heterogeneous acid and basic catalysts.More information on the use of enzymes in biodiesel production can be found in recent papers.10,12–14Methods b and c seem to be more attractive.Concerning method b s use of an acid catalyst for both esterification and transesterification reactions s Zhang et al.16,17recently showed,by a technological assessment of different continuous processes,that the homogeneous H 2SO 4-catalyzed process using waste oil is technically feasible and less complex than a two-step process (pre-esterification with homogeneous acid catalyst and alkali-catalyzed steps).However,this process gives rise to problems linked with the corrosive action of the liquid acid catalyst and to the high quantity of byproduct obtained.5,15Basu and Norris 18and more recently Di Serio et al.15and Siano et al.19showed the possibility to perform triglyceride (TG)transesterification and FFA esterification in a single step,using low concentrations of Lewis acid homoge-neous catalysts (carboxylic acid of particular metals).However,this process also gives rise to problems linked with the need to remove catalysts from products by downstream purification.15A solid acid catalyst could eliminate these problems.5,8The homogeneous acid-catalyzed pre-esterification of FFA s method c s is a common practice in reducing FFA levels in high FFA feedstock,before performing the base-catalyzed transesteri-fication.4,5,10The main drawback of the pre-esterification method c consists again in the necessity to remove the homogeneous acid catalyst from the oil after pre-esterification.So for improving the process,the solution is again the use of a heterogeneous esterification catalyst.5From the above discussion,it is clear that the introduction of a solid catalyst in biodiesel production could reduce its price,so biodiesel could become competitive with diesel also from an economic point of view.Therefore,great research efforts have been underway recently to find the right catalysts.Several general reviews on biofuels and biodiesel production have been published:3–7,10one concerns both homogeneous and heterogeneous acid catalysts.5In the second part of a general review on biodiesel production,Lotero et al.20also included an in-depth review on heterogeneous catalysts used in transesteri-fication reactions.Together with the scientific and patent literature concerning biodiesel production,in some cases,we have also considered results reported on the transesterification or esterification of model molecules like triacetin or acetic acid to explain the reaction mechanisms and catalytic behavior of heterogeneous catalysts.However,it must be pointed out that in some cases the data obtained with model molecules cannot be used to predict the behavior of oils/fats and fatty acids because the polar and steric effects of the alpha-substituent group can greatly influence the reactivity.21Heterogenous CatalystsReaction Mechanisms:A General Overview.Heteroge-neous acid and basic catalysts could be classified as Brönsted or Lewis catalysts,though in many cases both types of sites could be present and it is not easy to evaluate the relative importance of the two types of sites in the reaction.A detailed description of some reaction mechanisms can be found in the paper of Lotero et al.20Here for brevity’s sake,we will report only a general overview on reaction mechanisms of the different catalyst types.When homogeneous Brönsted basic catalysts,i.e.,NaOH,KOH,Na 2CO 3,were mixed with alcohol,the actual catalyst is formed.This is the alkoxide group:Na +OH -+CH 3OH f H 2O +CH 3O -Na +which attacks the carbonyl carbon atom of the triglyceride molecule.3,20Often an alkoxide (NaOCH 3,KOCH 3)is directly used as catalyst.A similar mechanism is operative in the case of a heteroge-neous basic Bronsted catalyst such as basic zeolite:20Also inthis case,the formed catalytic specie is a homogeneous alkoxide.In the case of heterogeneous basic Bronsted catalyst such as resin with quaternary ammonium functionality (QN +OH -),the positive counterions (organic ammonium groups),being bonded(11)Ono,T.,Yoshiharu,K.Process for producing lower alcohol esters or fatty acids.U.S.Patent 4,164,506,Aug 14,1979.(12)Fukuda,H.;Kondo,A.;Noda,H.J.Biosci.Bioeng.2001,92,405–416.(13)Haas,M.J.;Piazza,G.J.;Foglia,T.A.Lipid Biotechol.2002,587–598.(14)Kumari,V.;Shah,S.;Gupta,M.N.Energy Fuels 2007,21,368–372.(15)Di Serio,M.;Tesser,R.;Dimiccoli,M.;Cammarota,F.;Nastasi,M.;Santacesaria,E.J.Mol.Catal.A:Chem.2005,239,111–115.(16)Zhang,Y.;Dubè,M.A.;McLean,D.D.;Kates,M.Bioresour.Technol.2003,89,1–16.(17)Zhang,Y.;Dubè,M.A.;McLean,D.D.;Kates,M.Bioresour.Technol.2003,90,229–240.(18)Basu,H.N.;Norris,M.E.U.S.Patent 5,525,126,June 11,1996.(19)Siano,D.;Di Serio,M.;Tesser,R.;Dimmicoli,M.;Cammarota,F.;Santacesaria,E.;Siano,L.;Nastasi,M.Process for the production of esters from V egetables oils or animal fats.PCT No.WO2006/006033,Jan 19,2006.(20)Lotero,E.;Goodwin,J.G.,Jr.;Bruce,D.A.;Suwannakarn,K.;Liu,Y.;Lopez,D.E.Catalysis 2006,19,41–84.(21)Liu,Y.;Lotero,E.;Goodwin,J.G.,Jr.J.Catal.2006,243,221–228.208Energy &Fuels,Vol.22,No.1,2008Di Serio et al.directly to the support surface,electronically retain the catalytic anions on the solid surface:The reaction occurs betweenmethanol adsorbed on the cation and ester from the liquid (Eley–Rideal mechanism).The formation of alkoxide groups is also a fundamental step for heterogeneous basic Lewis catalyst.For example,in the case of ethylacetate transesterification,catalyzed by MgO,the reaction occurs between the methanol molecules adsorbed on a magnesium oxide free basic sites and the ethyl acetate moleculesfrom the liquid phase (Eley–Rideal mechanism).22,23Both homogeneous Brönsted (H 2SO 4,p -toluensolfonic acid 5,20)and Lewis (metal acetate,10,15,18–20metal complexes 24)acid catalysts have been used in biodiesel synthesis,and both catalyze either transesterification and esterification reactions.25Brönsted acid catalysts are active mainly in esterification reactions while Lewis acid catalysts are more active in transesterification reactions (see,for example,Table 126).Table 1reports data of runs performed by using a series of small stainless steel vial reactors.Both the reagents (oil (FFA )0.2%w/w))2.0g,methanol )0.88g)and a specified amount of the catalyst were introduced into each reactor.All the reactors were then heated in a ventilated oven.The temperature of the oven was initially fixed at 50°C for 14min and then increased at a rate of 20°C/min until the reaction temperature was reached,where the samples were kept for the fixed reaction time.At the end of the reaction,the temperature was quenched by putting the vials in a cold bath.Experimental runs were also performed by adding oleic acid to the reaction mixture.Oleic acid has been chosen as a test molecule for simulating the behavior of FFA.The lead acetate (Lewis acid)has greater transesterification activity than p -toluenesulfonic (Brønsted acid)acid,while on the contrary p -toluenesulfonic acid is more active than lead acetate in esterification reactions.The lower yield obtained with lead acetate using an acid oil is justified by the strong deactivation of the Lewis catalyst due to the water formed in the esterification reaction.15In both homogeneous and heterogeneous Brønsted acid catalysis,the mechanism pathways proceed through the protonation of the carbonyl group:increasing its electrophi-licity and rendering it more susceptible to alcohol nucleo-philic attack.21In the case of Nafion supported on silica (Nafion SAC-13),Lopez et al.27proposed a mechanistic pathway for triacetin transesterification s a mechanism similar to that accepted for a homogeneous Brønsted acid catalyst.They found activation energy and reaction orders similar to the ones showed by sulfuric acid.27The reaction mechanism in esterification reactions promoted by solid acid Brønsted catalysts is also similar to the homoge-neous one.28Liu et al.,28found that Nafion supported on silica has comparable turnover frequencies (TOF)to H 2SO 4and a similar reaction mechanism in esterification of the liquid acetic acid with methanol.The reaction occurs via a single-site Eley–Rideal mechanism involving a nucleophilic attack between adsorbed carboxylic acid and unadsorbed alchohol as the rate-determining step.28The formation of a more electrophilic species also occurs with homogeneous and heterogeneous Lewis acid catalysts as the first step in the reaction mechanism:15,29–31In this case,therate-determining step depends on the Lewis catalyst’s acid strength.After the Lewis complex formation (stage 1),the alcohol nucleophilic bonding (stage 2),and the new ester formation (stage 3),the new ester desorbs from the Lewis site (stage 4)and the cycle is repeated.If the strength of acidic sites is too high,the desorption of the product is not favored,(22)Dossin,T.F.;Reyniers,M.F.;Marin,G.B.Appl.Cat.B.2006,61,35–45.(23)Dossin,T.F.;Reyniers,M.F.;Berger,R.J.;Marin,G.B.Appl.Cat.B.En V iron.2006,67,136–148.(24)Abreu,F.R.;Lima,D.G.;Hamù,D.G.;Einloft,S.;Rubim,J.C.;Suarez,P.A.Z.J.Am.Oil Chem.Soc.2003,80,601–604.(25)López,D.E.;Suwannakarn,K.;Bruce,D.A.;Goodwin,J.G.,Jr.J.Cat.2007,247,43–50.(26)DiSerio,M.;Tesser,R.;Mandato,N.;Santacesaria,E.Unpublished data.(27)López,D.E.;Goodwin,J.G.,Jr.;Bruce,D.A.J.Cat.2007,245,381–391.(28)Liu,Y.;Lotero,E.;Goodwin,J.G.,Jr.J.Cat.2006,242,278–286.(29)Parshall,G.W.Ittel SI Homogeneous Catalysis:The Applications and Chemistry of Catalysis by Soluble Transition Metal ,2nd ed.;Wiley-Interscience:New York,2005.(30)Di Serio,M.;Apicella,B.;Grieco,G.;Iengo,P.;Fiocca,L.;Po,R.;Santacesaria,E.J.Mol.Cat.A.Chem.1998,130,233–240.(31)Bonelli,B.;Cozzolino,M.;Tesser,R.;Di Serio,M.;Piumetti,M.;Garrone,E.;Santacesaria,E.J.Cat.2007,246,293–300.Table 1.Oil )Soybean,8g;Methanol/Oil Molar Ratio )12:126catalyst initial FFA conc (%wt)reaction temp (°C)reaction time (min)cat (mol)FAME yield (%)final FFA conc (%wt)uncatalyzed 180402uncatalyzed 20.5180602210.7Pb(Ac)218040 5.4710-592p -toluenesulfonic acid 18040 5.3510-529Pb(Ac)220.518060 5.3810-557 6.8p -toluenesulfonic acid20.5180606.4010-5481.1Catalysts for Biodiesel Production Energy &Fuels,Vol.22,No.1,2008209determining a slow reaction rate.15,29–31This mechanism was confirmed for both homogeneous15,29,30and heterogeneous catalysts31by the observation that an optimal range of strength for Lewis acidic sites exists and that very strong Lewis acidic catalysts are less active in transesterification reactions.15,29–31 Basic Catalysts.Gryglewicz32investigated the possibility of using alkaline-earth metal hydroxides,oxides,and alkoxides to catalyze the transesterifiction of rapeseed oil at methanol reflux temperature.He found that sodium hydroxide was the most active,barium hydroxide was slightly less active,and that calcium methoxide showed medium activity.The reaction rate was lowest when CaO powder was used as catalyst while magnesium oxide and calcium hydroxide showed no catalytic activity.32The high activity of barium hydroxide is due to its higher solubility in methanol with respect to other compounds. The order of reactivity Ca(OH)2<CaO<Ca(CH3O)2agrees with the Lewis basic theory:the methoxides of alkaline-earth metals are more basic than their oxides which are more basic than their hydroxides.32Table2reports some data about CaO catalytic performance.32,33The yield of biodiesel using CaO as a catalyst increases with increase in the temperature and methanol/oil molar ratio,especially in the case of the methanol supercritical state33,34s see Table3entries1–3.In the methanol supercritical state,good performances were obtained also with Ca(OH)2and CaCO334s see Table3entries4–6.Increases in CaO performances can be obtained using nanocrystalline calcium oxides.35The nanocrystalline calcium oxides(crystal size)20nm;specific surface area)90m2/g) give100%conversion of soybean oil at room temperature after 12h while the conversion obtained with commercial CaO (crystal size)43nm;specific surface area)1m2/g)is only 2%.35López Granados et al.36studied the activity of activated CaO as a catalyst in the production of biodiesel by the transesteri-fication of triglycerides with methanol.The active surface sites of CaO are poisoned by the atmospheric H2O and CO2.The catalytic activity of CaO can be improved if CaO is subjected to an activation treatment at high temperature(g700°C)before the reaction s to remove the main poisoning species(the carbonate groups)from the surface s and if the contact with atmospheric air is prevented after this treatment.36Even if the catalyst can be reused for several runs without significant deactivation,dissolution of CaO does occur.The catalytic reaction is the result of the contribution of both heterogeneous and homogeneous catalysis for the formation of leached active species and further investigation is necessary to quantify this aspect.36The transesterification rate can be increased using microwave energy,37,38because the microwave energy selectively energizes the catalyst’s interaction with the reactants.38Table4(entries1and2)reports results obtained with Ca(OH)238and Ba(OH)2.37The data of Mazzochia et al.37 confirm that Ba(OH)2is not a completely heterogeneous catalyst. As a matter of fact,when the product obtained after reaction is not washed several times with distilled water,the resulting FAME and glycerine contain ca.0.06%and0.25%of barium, respectively.Good results in transesterification of soybean oil were obtained using ZnO loaded Sr(NO3)2followed by calcination at873K for5h.39When the transesterification reaction was carried out at reflux of methanol(65°C),with a12:1molar ratio of methanol to soybean oil and a catalyst amount of5wt %,the conversion of soybean oil was94.7%.39The SrO derived from thermal decomposition of Sr(NO3)2at high calcination temperatures is probably the main catalytically active specie.39 However,the used catalyst was significantly deactivated and could not be directly reused for transesterification.39Yang and Xie39explained the deactivation by the deposition of reactants and products on the active sites and/or by a transformation of the active sites and their interactions during the reaction. However,the leaching of SrO was not examined;notwithstand-ing,its high solubility in the reaction environment36is known. Sodium silicate catalyzes the transesterification of oils with high rates at moderate temperatures(60–120°C)s even if no data on catalyst reusability was reported.38Also in this case, the use of microwave energy and high methanol/oil ratio greatly increases the performances s see Table4entries3and4. Corma et al.,40in a patent mainly devoted to the transesteri-fication of triglycerides with glycerol to prepare monoglycerides, claimed the possibility to use calcined hydrotalcites and magnesium oxides in promoting the transesterification of triglycerides with monoalcohols,even if no examples or experimental data for this reaction are reported in the mentioned patent.Leclercq et al.41tested the use of commercial MgO/Al2O3 hydrotalcites and MgO(300m2/g)in the transesterification of rapeseed oil.They found that MgO was more active than hydrotalcite s see Tables5and6entry1.On the other hand, Cantrell et al.,42successfully used calcined hydrotalcites in promoting the transesterification of glycerol tributyrate with methanol at60°C.The rate increases steadily with Mg content, and the most active catalyst Al/(Mg+Al))0.25was10times more active than MgO.42Xie et al.,43for soybean oil transes-terification at methanol reflux,found that the most active calcined hydrotalcite has again an atomic ratio Al/(Mg+Al) )0.25s see Table6entries2–4s and that a higher active solid is obtained by calcining it at500°C;see Table6entries3,5, and6.Di Serio et al.44and Siano et al.45showed the possibility(32)Gryglewicz,S.Bioresour.Technol.1999,70,249–253.(33)Demirbas,A.Energy Con V ers.Manage.2007,48,937–941.(34)Tateno T.,Sasaki T.Process for producing fatty acid fuels comprising fatty acids esters.U.S.Patent6,818,026,Nov16,2004.(35)Reddy,C.;Reddy,V.;Oshel,R.;Verkade,J.G.Energy Fuels2006, 20,1310–1314.(36)Lopez Granados,M.;Zafra Poves,M.D.;Martin Alonso,D.; Mariscal,R.;Cabello Galisteo,F.;Moreno Tost,R.;Santamaria,J.;Fierro, J.L.G.App.Cat.B.2007,73,317–326.(37)Mazzocchia,C.;Modica,G.;Nannicini,R.;Kaddouri,A.C.R. Chim.2004,7,601–605.(38)Portnoff,M.A.;Purta,D.A.;Nasta,M.A.;Zhang,J.Pourarian,F.Methods for producing biodiesel.PCT No.WO2006/002087,Jan5,2006.(39)Yang,Z.Q.;Xie,W.L.Fuel Process.Technol.2007,88,631–638.(40)Corma,A.,Iborra,S.,Miquel,S.,Primo Millo,J.Process and Catalysts for the Selective production of Esters of fatty Acids.PCT No WO98/56747,Dec17,1998.(41)Leclercq,E.;Finiels,A.;Moreau,C.J.Amer.Oil Chem.Soc.2006, 78,1161–1165.(42)Cantrell,D.G.;Gillie,L.J.;Lee,A.F.;Wilson,K.Appl.Catal., A2005,287,183–1990.(43)Xie,W.;Peng,H.;Chen,L.J.Mol.Cat.A.2006,246,24–32.(44)Di Serio,M.;Ledda,M.;Cozzolino,M.;Minutillo,G.;Tesser,R.; Santacesaria,E.Ind.Eng.Chem.Res.2006,45,3009–3014.Table2.FAME Yield Using CaO as Catalyst at15min ofReaction Timeref oilreactiontemp(°C)methanol/oilmolar ratiocat conc(%w/w)FAMEyield(%)32rapeseed methanol reflux 4.5:10.81033sunflower19241.1:1 3.050210Energy&Fuels,Vol.22,No.1,2008Di Serio et al.to use calcined hydrotalcites and MgO for industrial biodiesel production at moderately high temperature.High yields of methyl esters were obtained in1h of reaction time at180–200°C s see Tables5(entries2–3)and6(entry7).At least four different types of basic sites have been indentified on the surface of MgO and calcined hydrotalcite catalysts.44The strongest basic sites(superbasic)promote the transesterification reaction also at very low temperatures(100°C),while the basic sites of medium strength require higher temperatures to promote the same reaction.44The experimental data reported show a correlation not only with the catalyst basicity but also with its structural texture.44However,the structural texture of the catalysts examined is dependent on both the precursor and the preparation method.44,46MgO catalyst used to promote transesterification strongly increases the reaction rate in supercritical conditions,34as can be seen in Table3entry7.As vegetable oils,animal fats,and alcohols usually contain water,44the influence of the presence of water on MgO and calcined hydrotacite performances have also been investigated.44,45 Some runs have been performed at180°C in the presence of high water concentration(10000ppm).44The results show that the activity of both magnesium oxide and calcined hydrotalcite is not affected by the presence of an excess of water.44This lastfinding is relevant for industrial purposes,because the possibility to operate in the presence moisture reduces the raw material pretreatment costs and opens the possibility to use unrefined bioethanol.However,Oku et al.47showed that in a run performed at150°C with60g of triolein,20g of methanol,and2.5g of Mg/Al hydrotalcite after24h of reaction,a FAME yield of77was produced,but a very high concentration of Mg and Al ions were detected in the products(Mg17800ppm;Al6900ppm).So, the problems of catalyst leaching need more in-depth study to confirm the possibility of using MgO and related hydrotalcites as industrial catalysts.Corma et al.48have reported that calcined Li/Al hydrotalcites are more active in glycerolysis of fatty acid methyl esters than the Mg/Al material(or MgO)due to their higher Lewis basicity. Starting from this observation,Shumaker et al.49studied the transesterification of soybean oil to fatty acid methyl esters using a calcined Li/Al layered double hydroxide catalyst.It was found that,at the reflux temperature of methanol,near-quantitative conversion of the soybean oil was achieved at low catalyst loadings(2–3wt%)and short reaction times(∼2h).49Catalyst recycling runs showed that the catalyst maintained a high level(45)Siano,D.;Nastasi,M.;Santacesaria,E.;Di Serio,M.;Tesser,R.; Minutillo,G.;Ledda,M.;Tenore,T.Process for Producing esters from vegetable oils or animal fats using heterogeneous catalysts.PCT Application No WO2006/050925,May18,2006.(46)Choudhary,V.R.;Pandit,M.Y.Appl.Catal.1991,71,265–274.(47)Oku,T.;Nonoguchi,M.;Moriguchi,T.Method of producing of fatty alkyl esters and/or glycerine and fatty acid alkyl ester-containing composition.PCT Application No WO2005/021697,Mar10,2005.(48)Corma,A.;Hamid,S.B.A.;Iborra,S.;Velty,A.J.Catal.2005, 234,340–347.(49)Shumaker,J.L.;Crofcheck,C.;Tackett,S.A.;Santillan-Jimenez,E.;Crocker,M.Cat.Lett.2007,115,56–61.Table3.FAME Yield Using Basic Catalysts in Methanol Supercritical Conditionsentry ref oil catalystreactiontemp(°C)methanol/oilmolar ratioreacttime(min)cat conc(%w/w)FAMEyield(%)133sunflower CaO252 6.0:115 3.065 233sunflower CaO25241.1:115 3.099 334soybean CaO30039.3:1100.5897 434soybean CaCO325039.3:110 1.1487 534soybean CaCO330039.3:1100.6799 634soybean Ca(OH)230039.4:1100.6898 734soybean MgO30039.6:110 1.2991 parison of the Transesterification Tests of Oil with Methyl Alcohol Carried out under Microwave Irradiationentry ref oil catalystreactiontemp(°C)methanol/oilmolar ratioreactiontime(min)cat conc(%w/w)FAMEyield(%)137rapeseed Ba(OH)2methanol reflux9:1150.597–98 238soybean Ca(OH)21006:120 2.081 338castor oil sodium silicate1206:110 1.570 438castor oil sodium silicate12019:110 1.5100Table5.FAME Yield Using MgO as Catalystentry ref oil surfaceareareactiontemp(°C)methanol/triglyceridesmolar ratioreactiontime(h)cat conc(%w/w)FAMEyield(%)141rapeseed300methanol reflux75:1221064 244soybean3618012:11 5.072 344soybean22918012:11 5.090Table6.FAME Yield Using Calcined MgO/Al2O3as Catalystentry ref oil Al/(Mg+Al)surfaceareacalcinationtemp(°C)reactiontemp(°C)methanol/oilmolarratioreactiontime(h)cat conc(%w/w)FAMEyield(%)141rapeseed0.30160450methanol reflux275:1221034 243soybean0.28500methanol reflux15:197.528 343soybean0.25500methanol reflux15:197.566 443soybean0.22500methanol reflux15:197.550 543soybean0.25450methanol reflux15:197.545 643soybean0.25600methanol reflux15:197.557 744soybean0.2514450018012:11 5.092 Catalysts for Biodiesel Production Energy&Fuels,Vol.22,No.1,2008211。
鉴定丝织品年代有了新方法
鉴定丝织品年代有了新方法环球科技观光团 2011-10-16 17:55:20传统的碳鉴年法需要的样本量太大,用在丝织品上几乎就等于把织物毁坏了。
而现在,一种超级灵敏的化 学手段可能可以解决丝织品鉴年的问题。
图为 18 世纪的法国丝织品在博物馆收藏的那些珍贵又脆弱的工艺品中,丝织品的年代鉴定显得比较困难.历史学家常 常需要借助周边环境中的证据,甚至是猜测来为古代丝织衣物定下一个制造年代。
这是由于 传统的碳鉴年法需要的样本量太大,用在丝织品上几乎就等于把织物毁坏了。
而现在,一种 超级灵敏的化学手段可能可以解决丝织品鉴年的问题。
化学家麦迪·莫伊尼(Mehdi Moini)多年来一直在研究一种能测量蛋白质材料自然分解的 技术。
这一技术足够灵敏,能够测量以小时计算的分解。
可以当作测定丝绸这类蛋白质材料 的分子钟。
不过先前他一直没有机会找到已经得到严格鉴年的丝制品来对这个“钟”进行校 准。
不过, 去年莫伊尼加入了美国史密森博物馆保护学会 (Smithsonian Museum Conservation Institute),得到了接触博物馆著名丝制品藏品的机会。
莫伊尼的技术依赖于蛋白质中的氨基酸从左旋形态转为右旋镜像形态的趋势。
这一转化不常 发生,不过确实以稳定速率在进行。
蚕吐丝之后过的时间越久,2 种类型氨基酸之间的比率 就越大。
通过 2 种化学技术手段,只需微量丝绸样本,用 20 分钟就可以测出这个比率。
目前史密森博物馆和其它几家博物馆已经同意让莫伊尼的研究小组采样丝制品, 包括古代中 国丝织品、 法国文艺复兴时期织品以及美国内战时的旗帜。
历史记录提供了博物馆藏品的明 晰制造日期,而通过这个技术鉴定出的年代误差在 50-100 年内。
研究结果已经发表于《分 析化学》(Analytical Chemistry)杂志。
美国阿克伦大学生物学家布莱克莱吉(Todd Blackledge)表示,这个研究不仅仅能用于鉴 别赝品。
微生物英文文献及翻译—原文
Dynamic and distribution of ammonia-oxidizing bacteria communities during sludge granulation in an anaerobic e aerobic sequencing batch reactorZhang Bin a ,b ,Chen Zhe a ,b ,Qiu Zhigang a ,b ,Jin Min a ,b ,Chen Zhiqiang a ,b ,Chen Zhaoli a ,b ,Li Junwen a ,b ,Wang Xuan c ,*,Wang Jingfeng a ,b ,**aInstitute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,PR China bTianjin Key Laboratory of Risk Assessment and Control for Environment and Food Safety,Tianjin 300050,PR China cTianjin Key Laboratory of Hollow Fiber Membrane Material and Membrane Process,Institute of Biological and Chemical Engineering,Tianjin Polytechnical University,Tianjin 300160,PR Chinaa r t i c l e i n f oArticle history:Received 30June 2011Received in revised form 10September 2011Accepted 10September 2011Available online xxx Keywords:Ammonia-oxidizing bacteria Granular sludgeCommunity development Granule sizeNitrifying bacteria distribution Phylogenetic diversitya b s t r a c tThe structure dynamic of ammonia-oxidizing bacteria (AOB)community and the distribution of AOB and nitrite-oxidizing bacteria (NOB)in granular sludge from an anaerobic e aerobic sequencing batch reactor (SBR)were investigated.A combination of process studies,molecular biotechniques and microscale techniques were employed to identify and characterize these organisms.The AOB community structure in granules was substantially different from that of the initial pattern of the inoculants sludge.Along with granules formation,the AOB diversity declined due to the selection pressure imposed by process conditions.Denaturing gradient gel electrophoresis (DGGE)and sequencing results demonstrated that most of Nitrosomonas in the inoculating sludge were remained because of their ability to rapidly adapt to the settling e washing out action.Furthermore,DGGE analysis revealed that larger granules benefit more AOB species surviving in the reactor.In the SBR were various size granules coexisted,granule diameter affected the distribution range of AOB and NOB.Small and medium granules (d <0.6mm)cannot restrict oxygen mass transfer in all spaces of the rger granules (d >0.9mm)can result in smaller aerobic volume fraction and inhibition of NOB growth.All these observations provide support to future studies on the mechanisms responsible for the AOB in granules systems.ª2011Elsevier Ltd.All rights reserved.1.IntroductionAt sufficiently high levels,ammonia in aquatic environments can be toxic to aquatic life and can contribute to eutrophica-tion.Accordingly,biodegradation and elimination of ammonia in wastewater are the primary functions of thewastewater treatment process.Nitrification,the conversion of ammonia to nitrate via nitrite,is an important way to remove ammonia nitrogen.It is a two-step process catalyzed by ammonia-oxidizing and nitrite-oxidizing bacteria (AOB and NOB).Aerobic ammonia-oxidation is often the first,rate-limiting step of nitrification;however,it is essential for the*Corresponding author .**Corresponding author.Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,PR China.Tel.:+862284655498;fax:+862223328809.E-mail addresses:wangxuan0116@ (W.Xuan),jingfengwang@ (W.Jingfeng).Available online atjournal homepage:/locate/watresw a t e r r e s e a r c h x x x (2011)1e 100043-1354/$e see front matter ª2011Elsevier Ltd.All rights reserved.doi:10.1016/j.watres.2011.09.026removal of ammonia from the wastewater(Prosser and Nicol, 2008).Comparative analyses of16S rRNA sequences have revealed that most AOB in activated sludge are phylogeneti-cally closely related to the clade of b-Proteobacteria (Kowalchuk and Stephen,2001).However,a number of studies have suggested that there are physiological and ecological differences between different AOB genera and lineages,and that environmental factors such as process parameter,dis-solved oxygen,salinity,pH,and concentrations of free ammonia can impact certain species of AOB(Erguder et al., 2008;Kim et al.,2006;Koops and Pommerening-Ro¨ser,2001; Kowalchuk and Stephen,2001;Shi et al.,2010).Therefore, the physiological activity and abundance of AOB in waste-water processing is critical in the design and operation of waste treatment systems.For this reason,a better under-standing of the ecology and microbiology of AOB in waste-water treatment systems is necessary to enhance treatment performance.Recently,several developed techniques have served as valuable tools for the characterization of microbial diversity in biological wastewater treatment systems(Li et al., 2008;Yin and Xu,2009).Currently,the application of molec-ular biotechniques can provide clarification of the ammonia-oxidizing community in detail(Haseborg et al.,2010;Tawan et al.,2005;Vlaeminck et al.,2010).In recent years,the aerobic granular sludge process has become an attractive alternative to conventional processes for wastewater treatment mainly due to its cell immobilization strategy(de Bruin et al.,2004;Liu et al.,2009;Schwarzenbeck et al.,2005;Schwarzenbeck et al.,2004a,b;Xavier et al.,2007). Granules have a more tightly compact structure(Li et al.,2008; Liu and Tay,2008;Wang et al.,2004)and rapid settling velocity (Kong et al.,2009;Lemaire et al.,2008).Therefore,granular sludge systems have a higher mixed liquid suspended sludge (MLSS)concentration and longer solid retention times(SRT) than conventional activated sludge systems.Longer SRT can provide enough time for the growth of organisms that require a long generation time(e.g.,AOB).Some studies have indicated that nitrifying granules can be cultivated with ammonia-rich inorganic wastewater and the diameter of granules was small (Shi et al.,2010;Tsuneda et al.,2003).Other researchers reported that larger granules have been developed with the synthetic organic wastewater in sequencing batch reactors(SBRs)(Li et al., 2008;Liu and Tay,2008).The diverse populations of microor-ganisms that coexist in granules remove the chemical oxygen demand(COD),nitrogen and phosphate(de Kreuk et al.,2005). However,for larger granules with a particle diameter greater than0.6mm,an outer aerobic shell and an inner anaerobic zone coexist because of restricted oxygen diffusion to the granule core.These properties of granular sludge suggest that the inner environment of granules is unfavorable to AOB growth.Some research has shown that particle size and density induced the different distribution and dominance of AOB,NOB and anam-mox(Winkler et al.,2011b).Although a number of studies have been conducted to assess the ecology and microbiology of AOB in wastewater treatment systems,the information on the dynamics,distribution,and quantification of AOB communities during sludge granulation is still limited up to now.To address these concerns,the main objective of the present work was to investigate the population dynamics of AOB communities during the development of seedingflocs into granules,and the distribution of AOB and NOB in different size granules from an anaerobic e aerobic SBR.A combination of process studies,molecular biotechniques and microscale techniques were employed to identify and char-acterize these organisms.Based on these approaches,we demonstrate the differences in both AOB community evolu-tion and composition of theflocs and granules co-existing in the SBR and further elucidate the relationship between distribution of nitrifying bacteria and granule size.It is ex-pected that the work would be useful to better understand the mechanisms responsible for the AOB in granules and apply them for optimal control and management strategies of granulation systems.2.Material and methods2.1.Reactor set-up and operationThe granules were cultivated in a lab-scale SBR with an effective volume of4L.The effective diameter and height of the reactor was10cm and51cm,respectively.The hydraulic retention time was set at8h.Activated sludge from a full-scale sewage treat-ment plant(Jizhuangzi Sewage Treatment Works,Tianjin, China)was used as the seed sludge for the reactor at an initial sludge concentration of3876mg LÀ1in MLSS.The reactor was operated on6-h cycles,consisting of2-min influent feeding,90-min anaerobic phase(mixing),240-min aeration phase and5-min effluent discharge periods.The sludge settling time was reduced gradually from10to5min after80SBR cycles in20days, and only particles with a settling velocity higher than4.5m hÀ1 were retained in the reactor.The composition of the influent media were NaAc(450mg LÀ1),NH4Cl(100mg LÀ1),(NH4)2SO4 (10mg LÀ1),KH2PO4(20mg LÀ1),MgSO4$7H2O(50mg LÀ1),KCl (20mg LÀ1),CaCl2(20mg LÀ1),FeSO4$7H2O(1mg LÀ1),pH7.0e7.5, and0.1mL LÀ1trace element solution(Li et al.,2007).Analytical methods-The total organic carbon(TOC),NHþ4e N, NOÀ2e N,NOÀ3e N,total nitrogen(TN),total phosphate(TP) concentration,mixed liquid suspended solids(MLSS) concentration,and sludge volume index at10min(SVI10)were measured regularly according to the standard methods (APHA-AWWA-WEF,2005).Sludge size distribution was determined by the sieving method(Laguna et al.,1999).Screening was performed with four stainless steel sieves of5cm diameter having respective mesh openings of0.9,0.6,0.45,and0.2mm.A100mL volume of sludge from the reactor was sampled with a calibrated cylinder and then deposited on the0.9mm mesh sieve.The sample was subsequently washed with distilled water and particles less than0.9mm in diameter passed through this sieve to the sieves with smaller openings.The washing procedure was repeated several times to separate the gran-ules.The granules collected on the different screens were recovered by backwashing with distilled water.Each fraction was collected in a different beaker andfiltered on quantitative filter paper to determine the total suspended solid(TSS).Once the amount of total suspended solid(TSS)retained on each sieve was acquired,it was reasonable to determine for each class of size(<0.2,[0.2e0.45],[0.45e0.6],[0.6e0.9],>0.9mm) the percentage of the total weight that they represent.w a t e r r e s e a r c h x x x(2011)1e10 22.2.DNA extraction and nested PCR e DGGEThe sludge from approximately8mg of MLSS was transferred into a1.5-mL Eppendorf tube and then centrifuged at14,000g for10min.The supernatant was removed,and the pellet was added to1mL of sodium phosphate buffer solution and aseptically mixed with a sterilized pestle in order to detach granules.Genomic DNA was extracted from the pellets using E.Z.N.A.äSoil DNA kit(D5625-01,Omega Bio-tek Inc.,USA).To amplify ammonia-oxidizer specific16S rRNA for dena-turing gradient gel electrophoresis(DGGE),a nested PCR approach was performed as described previously(Zhang et al., 2010).30m l of nested PCR amplicons(with5m l6Âloading buffer)were loaded and separated by DGGE on polyacrylamide gels(8%,37.5:1acrylamide e bisacrylamide)with a linear gradient of35%e55%denaturant(100%denaturant¼7M urea plus40%formamide).The gel was run for6.5h at140V in 1ÂTAE buffer(40mM Tris-acetate,20mM sodium acetate, 1mM Na2EDTA,pH7.4)maintained at60 C(DCodeäUniversal Mutation Detection System,Bio-Rad,Hercules,CA, USA).After electrophoresis,silver-staining and development of the gels were performed as described by Sanguinetti et al. (1994).These were followed by air-drying and scanning with a gel imaging analysis system(Image Quant350,GE Inc.,USA). The gel images were analyzed with the software Quantity One,version4.31(Bio-rad).Dice index(Cs)of pair wise community similarity was calculated to evaluate the similarity of the AOB community among DGGE lanes(LaPara et al.,2002).This index ranges from0%(no common band)to100%(identical band patterns) with the assistance of Quantity One.The Shannon diversity index(H)was used to measure the microbial diversity that takes into account the richness and proportion of each species in a population.H was calculatedusing the following equation:H¼ÀPn iNlogn iN,where n i/Nis the proportion of community made up by species i(bright-ness of the band i/total brightness of all bands in the lane).Dendrograms relating band pattern similarities were automatically calculated without band weighting(consider-ation of band density)by the unweighted pair group method with arithmetic mean(UPGMA)algorithms in the Quantity One software.Prominent DGGE bands were excised and dissolved in30m L Milli-Q water overnight,at4 C.DNA was recovered from the gel by freeze e thawing thrice.Cloning and sequencing of the target DNA fragments were conducted following the estab-lished method(Zhang et al.,2010).2.3.Distribution of nitrifying bacteriaThree classes of size([0.2e0.45],[0.45e0.6],>0.9mm)were chosen on day180for FISH analysis in order to investigate the spatial distribution characteristics of AOB and NOB in granules.2mg sludge samples werefixed in4%para-formaldehyde solution for16e24h at4 C and then washed twice with sodium phosphate buffer;the samples were dehydrated in50%,80%and100%ethanol for10min each. Ethanol in the granules was then completely replaced by xylene by serial immersion in ethanol-xylene solutions of3:1, 1:1,and1:3by volume andfinally in100%xylene,for10min periods at room temperature.Subsequently,the granules were embedded in paraffin(m.p.56e58 C)by serial immer-sion in1:1xylene-paraffin for30min at60 C,followed by 100%paraffin.After solidification in paraffin,8-m m-thick sections were prepared and placed on gelatin-coated micro-scopic slides.Paraffin was removed by immersing the slide in xylene and ethanol for30min each,followed by air-drying of the slides.The three oligonucleotide probes were used for hybridiza-tion(Downing and Nerenberg,2008):FITC-labeled Nso190, which targets the majority of AOB;TRITC-labeled NIT3,which targets Nitrobacter sp.;TRITC-labeled NSR1156,which targets Nitrospira sp.All probe sequences,their hybridization condi-tions,and washing conditions are given in Table1.Oligonu-cleotides were synthesized andfluorescently labeled with fluorochomes by Takara,Inc.(Dalian,China).Hybridizations were performed at46 C for2h with a hybridization buffer(0.9M NaCl,formamide at the percentage shown in Table1,20mM Tris/HCl,pH8.0,0.01% SDS)containing each labeled probe(5ng m LÀ1).After hybrid-ization,unbound oligonucleotides were removed by a strin-gent washing step at48 C for15min in washing buffer containing the same components as the hybridization buffer except for the probes.For detection of all DNA,4,6-diamidino-2-phenylindole (DAPI)was diluted with methanol to afinal concentration of1ng m LÀ1.Cover the slides with DAPI e methanol and incubate for15min at37 C.The slides were subsequently washed once with methanol,rinsed briefly with ddH2O and immediately air-dried.Vectashield(Vector Laboratories)was used to prevent photo bleaching.The hybridization images were captured using a confocal laser scanning microscope (CLSM,Zeiss710).A total of10images were captured for each probe at each class of size.The representative images were selected andfinal image evaluation was done in Adobe PhotoShop.w a t e r r e s e a r c h x x x(2011)1e1033.Results3.1.SBR performance and granule characteristicsDuring the startup period,the reactor removed TOC and NH 4þ-N efficiently.98%of NH 4þ-N and 100%of TOC were removed from the influent by day 3and day 5respectively (Figs.S2,S3,Supporting information ).Removal of TN and TP were lower during this period (Figs.S3,S4,Supporting information ),though the removal of TP gradually improved to 100%removal by day 33(Fig.S4,Supporting information ).To determine the sludge volume index of granular sludge,a settling time of 10min was chosen instead of 30min,because granular sludge has a similar SVI after 60min and after 5min of settling (Schwarzenbeck et al.,2004b ).The SVI 10of the inoculating sludge was 108.2mL g À1.The changing patterns of MLSS and SVI 10in the continuous operation of the SBR are illustrated in Fig.1.The sludge settleability increased markedly during the set-up period.Fig.2reflects the slow andgradual process of sludge granulation,i.e.,from flocculentsludge to granules.3.2.DGGE analysis:AOB communities structure changes during sludge granulationThe results of nested PCR were shown in Fig.S1.The well-resolved DGGE bands were obtained at the representative points throughout the GSBR operation and the patterns revealed that the structure of the AOB communities was dynamic during sludge granulation and stabilization (Fig.3).The community structure at the end of experiment was different from that of the initial pattern of the seed sludge.The AOB communities on day 1showed 40%similarity only to that at the end of the GSBR operation (Table S1,Supporting information ),indicating the considerable difference of AOB communities structures between inoculated sludge and granular sludge.Biodiversity based on the DGGE patterns was analyzed by calculating the Shannon diversity index H as204060801001201401254159738494104115125135147160172188Time (d)S V I 10 (m L .g -1)10002000300040005000600070008000900010000M L S S (m g .L -1)Fig.1e Change in biomass content and SVI 10during whole operation.SVI,sludge volume index;MLSS,mixed liquid suspendedsolids.Fig.2e Variation in granule size distribution in the sludge during operation.d,particle diameter;TSS,total suspended solids.w a t e r r e s e a r c h x x x (2011)1e 104shown in Fig.S5.In the phase of sludge inoculation (before day 38),H decreased remarkably (from 0.94to 0.75)due to the absence of some species in the reactor.Though several dominant species (bands2,7,10,11)in the inoculating sludge were preserved,many bands disappeared or weakened (bands 3,4,6,8,13,14,15).After day 45,the diversity index tended to be stable and showed small fluctuation (from 0.72to 0.82).Banding pattern similarity was analyzed by applying UPGMA (Fig.4)algorithms.The UPGMA analysis showed three groups with intragroup similarity at approximately 67%e 78%and intergroup similarity at 44e 62%.Generally,the clustering followed the time course;and the algorithms showed a closer clustering of groups II and III.In the analysis,group I was associated with sludge inoculation and washout,group IIwithFig.3e DGGE profile of the AOB communities in the SBR during the sludge granulation process (lane labels along the top show the sampling time (days)from startup of the bioreactor).The major bands were labeled with the numbers (bands 1e15).Fig.4e UPGMA analysis dendrograms of AOB community DGGE banding patterns,showing schematics of banding patterns.Roman numerals indicate major clusters.w a t e r r e s e a r c h x x x (2011)1e 105startup sludge granulation and decreasing SVI 10,and group III with a stable system and excellent biomass settleability.In Fig.3,the locations of the predominant bands were excised from the gel.DNA in these bands were reamplified,cloned and sequenced.The comparative analysis of these partial 16S rRNA sequences (Table 2and Fig.S6)revealed the phylogenetic affiliation of 13sequences retrieved.The majority of the bacteria in seed sludge grouped with members of Nitrosomonas and Nitrosospira .Along with sludge granula-tion,most of Nitrosomonas (Bands 2,5,7,9,10,11)were remained or eventually became dominant in GSBR;however,all of Nitrosospira (Bands 6,13,15)were gradually eliminated from the reactor.3.3.Distribution of AOB and NOB in different sized granulesFISH was performed on the granule sections mainly to deter-mine the location of AOB and NOB within the different size classes of granules,and the images were not further analyzed for quantification of cell counts.As shown in Fig.6,in small granules (0.2mm <d <0.45mm),AOB located mainly in the outer part of granular space,whereas NOB were detected only in the core of granules.In medium granules (0.45mm <d <0.6mm),AOB distributed evenly throughout the whole granular space,whereas NOB still existed in the inner part.In the larger granules (d >0.9mm),AOB and NOB were mostly located in the surface area of the granules,and moreover,NOB became rare.4.Discussion4.1.Relationship between granule formation and reactor performanceAfter day 32,the SVI 10stabilized at 20e 35mL g À1,which is very low compared to the values measured for activated sludge (100e 150mL g À1).However,the size distribution of the granules measured on day 32(Fig.2)indicated that only 22%of the biomass was made of granular sludge with diameter largerthan 0.2mm.These results suggest that sludge settleability increased prior to granule formation and was not affected by different particle sizes in the sludge during the GSBR operation.It was observed,however,that the diameter of the granules fluctuated over longer durations.The large granules tended to destabilize due to endogenous respiration,and broke into smaller granules that could seed the formation of large granules again.Pochana and Keller reported that physically broken sludge flocs contribute to lower denitrification rates,due to their reduced anoxic zone (Pochana and Keller,1999).Therefore,TN removal efficiency raises fluctuantly throughout the experiment.Some previous research had demonstrated that bigger,more dense granules favored the enrichment of PAO (Winkler et al.,2011a ).Hence,after day 77,removal efficiency of TP was higher and relatively stable because the granules mass fraction was over 90%and more larger granules formed.4.2.Relationship between AOB communities dynamic and sludge granulationFor granule formation,a short settling time was set,and only particles with a settling velocity higher than 4.5m h À1were retained in the reactor.Moreover,as shown in Fig.1,the variation in SVI 10was greater before day 41(from 108.2mL g À1e 34.1mL g À1).During this phase,large amounts of biomass could not survive in the reactor.A clear shift in pop-ulations was evident,with 58%similarity between days 8and 18(Table S1).In the SBR system fed with acetate-based synthetic wastewater,heterotrophic bacteria can produce much larger amounts of extracellular polysaccharides than autotrophic bacteria (Tsuneda et al.,2003).Some researchers found that microorganisms in high shear environments adhered by extracellular polymeric substances (EPS)to resist the damage of suspended cells by environmental forces (Trinet et al.,1991).Additionally,it had been proved that the dominant heterotrophic species in the inoculating sludge were preserved throughout the process in our previous research (Zhang et al.,2011).It is well known that AOB are chemoau-totrophic and slow-growing;accordingly,numerous AOBw a t e r r e s e a r c h x x x (2011)1e 106populations that cannot become big and dense enough to settle fast were washed out from the system.As a result,the variation in AOB was remarkable in the period of sludge inoculation,and the diversity index of population decreased rapidly.After day 45,AOB communities’structure became stable due to the improvement of sludge settleability and the retention of more biomass.These results suggest that the short settling time (selection pressure)apparently stressed the biomass,leading to a violent dynamic of AOB communities.Further,these results suggest that certain populations may have been responsible for the operational success of the GSBR and were able to persist despite the large fluctuations in pop-ulation similarity.This bacterial population instability,coupled with a generally acceptable bioreactor performance,is congruent with the results obtained from a membrane biore-actor (MBR)for graywater treatment (Stamper et al.,2003).Nitrosomonas e like and Nitrosospira e like populations are the dominant AOB populations in wastewater treatment systems (Kowalchuk and Stephen,2001).A few previous studies revealed that the predominant populations in AOB communities are different in various wastewater treatment processes (Tawan et al.,2005;Thomas et al.,2010).Some researchers found that the community was dominated by AOB from the genus Nitrosospira in MBRs (Zhang et al.,2010),whereas Nitrosomonas sp.is the predominant population in biofilter sludge (Yin and Xu,2009).In the currentstudy,Fig.5e DGGE profile of the AOB communities in different size of granules (lane labels along the top show the range of particle diameter (d,mm)).Values along the bottom indicate the Shannon diversity index (H ).Bands labeled with the numbers were consistent with the bands in Fig.3.w a t e r r e s e a r c h x x x (2011)1e 107sequence analysis revealed that selection pressure evidently effect on the survival of Nitrosospira in granular sludge.Almost all of Nitrosospira were washed out initially and had no chance to evolve with the environmental changes.However,some members of Nitrosomonas sp.have been shown to produce more amounts of EPS than Nitrosospira ,especially under limited ammonia conditions (Stehr et al.,1995);and this feature has also been observed for other members of the same lineage.Accordingly,these EPS are helpful to communicate cells with each other and granulate sludge (Adav et al.,2008).Therefore,most of Nitrosomonas could adapt to this challenge (to become big and dense enough to settle fast)and were retained in the reactor.At the end of reactor operation (day 180),granules with different particle size were sieved.The effects of variation in granules size on the composition of the AOBcommunitiesFig.6e Micrographs of FISH performed on three size classes of granule sections.DAPI stain micrographs (A,D,G);AOB appear as green fluorescence (B,E,H),and NOB appear as red fluorescence (C,F,I).Bar [100m m in (A)e (C)and (G)e (I).d,particle diameter.(For interpretation of the references to colour in this figure legend,the reader is referred to the web version of this article.)w a t e r r e s e a r c h x x x (2011)1e 108were investigated.As shown in Fig.5,AOB communities structures in different size of granules were varied.Although several predominant bands(bands2,5,11)were present in all samples,only bands3and6appeared in the granules with diameters larger than0.6mm.Additionally,bands7and10 were intense in the granules larger than0.45mm.According to Table2,it can be clearly indicated that Nitrosospira could be retained merely in the granules larger than0.6mm.Therefore, Nitrosospira was not present at a high level in Fig.3due to the lower proportion of larger granules(d>0.6mm)in TSS along with reactor operation.DGGE analysis also revealed that larger granules had a greater microbial diversity than smaller ones. This result also demonstrates that more organisms can survive in larger granules as a result of more space,which can provide the suitable environment for the growth of microbes(Fig.6).4.3.Effect of variance in particle size on the distribution of AOB and NOB in granulesAlthough an influence of granule size has been observed in experiments and simulations for simultaneous N-and P-removal(de Kreuk et al.,2007),the effect of granule size on the distribution of different biomass species need be revealed further with the assistance of visible experimental results, especially in the same granular sludge reactors.Related studies on the diversity of bacterial communities in granular sludge often focus on the distribution of important functional bacteria populations in single-size granules(Matsumoto et al., 2010).In the present study,different size granules were sieved,and the distribution patterns of AOB and NOB were explored.In the nitrification processes considered,AOB and NOB compete for space and oxygen in the granules(Volcke et al.,2010).Since ammonium oxidizers have a higheroxygen affinity(K AOBO2<K NOBO2)and accumulate more rapidly inthe reactor than nitrite oxidizers(Volcke et al.,2010),NOB are located just below the layer of AOB,where still some oxygen is present and allows ready access to the nitrite produced.In smaller granules,the location boundaries of the both biomass species were distinct due to the limited existence space provided by granules for both microorganism’s growth.AOB exist outside of the granules where oxygen and ammonia are present.Medium granules can provide broader space for microbe multiplying;accordingly,AOB spread out in the whole granules.This result also confirms that oxygen could penetrate deep into the granule’s core without restriction when particle diameter is less than0.6mm.Some mathematic model also supposed that NOBs are favored to grow in smaller granules because of the higher fractional aerobic volume (Volcke et al.,2010).As shown in the results of the batch experiments(Zhang et al.,2011),nitrite accumulation temporarily occurred,accompanied by the more large gran-ules(d>0.9mm)forming.This phenomenon can be attrib-uted to the increased ammonium surface load associated with larger granules and smaller aerobic volume fraction,resulting in outcompetes of NOB.It also suggests that the core areas of large granules(d>0.9mm)could provide anoxic environment for the growth of anaerobic denitrificans(such as Tb.deni-trificans or Tb.thioparus in Fig.S7,Supporting information).As shown in Fig.2and Fig.S3,the removal efficiency of total nitrogen increased with formation of larger granules.5.ConclusionsThe variation in AOB communities’structure was remarkable during sludge inoculation,and the diversity index of pop-ulation decreased rapidly.Most of Nitrosomonas in the inocu-lating sludge were retained because of their capability to rapidly adapt to the settling e washing out action.DGGE anal-ysis also revealed that larger granules had greater AOB diversity than that of smaller ones.Oxygen penetration was not restricted in the granules of less than0.6mm particle diameter.However,the larger granules(d>0.9mm)can result in the smaller aerobic volume fraction and inhibition of NOB growth.Henceforth,further studies on controlling and opti-mizing distribution of granule size could be beneficial to the nitrogen removal and expansive application of granular sludge technology.AcknowledgmentsThis work was supported by grants from the National Natural Science Foundation of China(No.51108456,50908227)and the National High Technology Research and Development Program of China(No.2009AA06Z312).Appendix.Supplementary dataSupplementary data associated with this article can be found in online version at doi:10.1016/j.watres.2011.09.026.r e f e r e n c e sAdav,S.S.,Lee, D.J.,Show,K.Y.,2008.Aerobic granular sludge:recent advances.Biotechnology Advances26,411e423.APHA-AWWA-WEF,2005.Standard Methods for the Examination of Water and Wastewater,first ed.American Public Health Association/American Water Works Association/WaterEnvironment Federation,Washington,DC.de Bruin,L.M.,de Kreuk,M.,van der Roest,H.F.,Uijterlinde,C., van Loosdrecht,M.C.M.,2004.Aerobic granular sludgetechnology:an alternative to activated sludge?Water Science and Technology49,1e7.de Kreuk,M.,Heijnen,J.J.,van Loosdrecht,M.C.M.,2005.Simultaneous COD,nitrogen,and phosphate removal byaerobic granular sludge.Biotechnology and Bioengineering90, 761e769.de Kreuk,M.,Picioreanu,C.,Hosseini,M.,Xavier,J.B.,van Loosdrecht,M.C.M.,2007.Kinetic model of a granular sludge SBR:influences on nutrient removal.Biotechnology andBioengineering97,801e815.Downing,L.S.,Nerenberg,R.,2008.Total nitrogen removal ina hybrid,membrane-aerated activated sludge process.WaterResearch42,3697e3708.Erguder,T.H.,Boon,N.,Vlaeminck,S.E.,Verstraete,W.,2008.Partial nitrification achieved by pulse sulfide doses ina sequential batch reactor.Environmental Science andTechnology42,8715e8720.w a t e r r e s e a r c h x x x(2011)1e109。
Investigations of Particle Velocities in a Slurry Pump Using PIV
Jaikrishnan R.Kadambi Pathom CharoenngamAmirthaganeshSubramanian Department of Mechanical and AerospaceEngineering,Case Western Reserve University,10900Euclid Ave.,Cleveland,OH44106Mark P.Wernet National Aeronautics and Space Administration,John H.Glenn Research Center,21000Brookpark Rd.,Cleveland,OH44135John M.Sankovic Department of Biomedical Engineering,Case Western Reserve University and National Aeronautics and Space Administration,John H.Glenn Research Center,21000Brookpark Rd.,Cleveland,OH44135Graeme AddieRobert CourtwrightGIW Industries,5000Wrightsboro Rd.,Grovetown,GA30813-9750Investigations of Particle Velocities in a Slurry Pump Using PIV:Part1,The Tongue and Adjacent Channel FlowTransport of solid-liquid slurries in pipeline transport over short and medium distances is very important in many industries,including mining related processes.The particle image velocimetry technique was successfully utilized to investigate the velocities and kinetic energyfluctuations of slurry particles at the tongue region of an optically-clear centrifu-gal pump.The experiments were conducted using500micron glass beads at volumetric concentrations of2.5%and5%and at pump speeds of725rpm and1000rpm.The fluctuation kinetic energy increased approximately200%to500%as the pump speed was increased from725rpm to1000rpm.The directional impingement mechanism is more significant at the pressure side of the blade,tongue and the casing.This mechanism becomes more important as the speed increases.This suggests that the impeller,tongue and the casing of the slurry pump can wear out quickly,especially with an increase in speed.In this paper the emphasis is on the tongue region.The random impingement mechanism caused by thefluctuation kinetic energy of the solids can play an important role on the erosion of the tongue area.͓DOI:10.1115/1.1786928͔IntroductionThe transport of solid-liquid slurries over short and medium distances via pipelines is very important in many industrial,min-ing,and fossil-energy related processes.The pump is a critical component of the slurry transport system.In most applications a centrifugal pump is used.Theflow of concentrated slurry is very complicated.Wear and corrosion in centrifugal pumps make it the most vulnerable component of the slurry pipeline.To improve the longevity and performance of the slurry pumps it is important to understand theflow through them.Due to the inherent difficulties associated with makingflow measurements in solid-liquid slurries only non-intrusive techniques can be used.These non-intrusive measuring techniques include acoustic ultrasound,magnetic reso-nance imaging,X-ray tomography,neutron radiography,particle image velocimetry͑PIV͒,laser Doppler anemometry͑LDA͒and holographic interferometry.However,some of the techniques used to visualize theflow have significant limitations.Miner͓1͔modeled theflowfield within the impeller volute using potentialflow theory and also conducted experiments using LDA.Water was used as thefluid.The comparison of blade to blade velocity profiles between the theoretical and the experimen-tal results were good.Liu et al.͓2,3͔used LDA and refractive index matching technique to measure velocity vectors in a cen-trifugal pump.A mixture of tetraline and turpentine was used as a workingfluid to match the refractive index of the pump casing made from acrylic.They observed that unlike a general well-guidedflow at close to designflow rate condition,the impeller flow departs from the curvature of the blade surfaces at off-design conditions which increases blade to blade variations of relative velocity.Dong et al.͓4,5͔used Particle Displacement Velocimetry ͑PDV͒technique to visualize theflow within the volute of a cen-trifugal pump.Neutrally buoyant particles of30m mean diam-eter were used as seed.They observed that although most of the blade effects occur near the impeller tip,they are not limited to this region.In addition they stated that the entireflux pulsating within the volute reaches a maximum when the blade lines up with the tip of the tongue.Paone et al.͓6͔used Particle Image Displacement Velocimetry͑PIDV͒to measure theflowfield in a diffuser of a centrifugal pump with clear plexiglas®casing and impeller.Experiments were performed with water as thefluid and metallic coated microspheres͑diameter4m,density2.6g/cm3) were used as seed particles.They identified the blade wake path. Oldenburg and Pap͓7͔also used PIV to study theflowfield in the impeller and casing of a plexiglas®casing centrifugal pump.The vanes of the impeller were cylindrically curved to obtain two-dimensionalflow in the impeller.Because of the difficulties associated with measurements in solid-liquid slurryflow in pumps only a few experimental studies are available in the open literature.Roco͓8͔obtained LDA mea-surements of two-phaseflow in a centrifugal slurry pump at low concentrations͑1%͒.Micron size tracers and0.8mm glass beadsContributed by the Petroleum Division for publication in the J OURNAL OF E N-ERGY R ESOURCES T ECHNOLOGY.Manuscript received by the Petroleum Division October2002;revised manuscript received November2003.Associate Editor: S.Shirazi.were used.Fluctuations in angular velocity up to 20%,radial ve-locity up to 90%and axial velocity up to 200%from their mean velocity components over various impeller angular positions were observed.Altobelli et al.͓9͔used nuclear magnetic resonance ͑NMR ͒for measuring velocity in slurry with solid concentration up to 39%by volume.However,the maximum flow velocity that could be measured was 0.25m/s.Roco and Addie ͓10͔developed a numerical model to calculate velocity,concentration and erosion wear in the casing of a centrifugal slurry pump.Empirical param-eters such as a slip factor of the impeller and the experimental ratio of erosion rate for the model were obtained from the avail-able experimental data.Some other studies of solid-liquid slurry flow in centrifugal pumps and pipelines include Roco et al.͓11–14͔,Wilson et al.͓15–17͔Shook and Roco ͓18͔,Addie ͓19͔,and Cader et al.͓20͔.It is an important,but challenging,task to obtain experimental data for higher solid concentration in a centrifugal slurry pump which will result in a better understanding of the flow behavior,pump performance and wear and erosion characteristics.Aslurry flow loop with an optically clear casing and impeller that facili-tates the use of non-intrusive laser based PIV for making two-phase flow measurements in the blade passages and the casing has been developed.In this paper the test results in the tongue region of the pump for a slurry made up of spherical glass particles at 2.5%and 5%volumetric concentrations in sodium iodide solution are presented.The Experimental Set-UpSlurry Pump Loop Facility.The slurry pump loop facility ͑developed by GIW Industries,Inc.͒is located in the Laser Flow Diagnostics Laboratory,Case Western Reserve University,Cleve-land,Ohio,U.S.A.The slurry pump loop facility,described in detail by Charoenngam ͓21͔,consists of 50.8mm I.D.tygon®tubing closed loop,except for a 2.1meter long,75mm I.D.PVC straight section upstream of the pump inlet that provides a swirl free inlet flow to the pump,a 0.6meter long,51mm I.D.PVC straight section at the pump discharge,and a 4.6-meter high,63mm I.D.vertical loop for measuring average concentration deliv-ered by the pump.In order to minimize particle deposition and unnecessary pump head loss in the system,there are no sharp sudden flow area changes.The flow in the loop is delivered by an optically clear centrifu-gal slurry pump.The pump has a transparent casing and a trans-parent three-blade impeller.Control of the timing of the PIV laser firing as a function of impeller blade position was provided by a combination of an optical encoder placed 5mm away from the shaft of the centrifugal pump and a reflective surface marker on the shaft and aligned with one of the blades of the impeller.A digital magnetic flowmeter with an accuracy of Ϯ2%and located downstream of the pump discharge provided the flow rate in theloop.A type K thermocouple was located in the fluid reservoir was used to monitor the fluid temperature to within Ϯ0.5°C.The Optically Clear Centrifugal Slurry Pump.The prime mover for driving the flow in the loop is a single stage,radially split centrifugal pump.The pump was specially designed to pro-vide optical access.The casing of the pump and the impeller ͑Fig.1͒are made from optically transparent acrylic.The ratio of pump casing inlet diameter to the discharge diameter is 2.35.The single-or end-suction impeller with shroud on both sides to enclose the liquid passages was installed in a semi volute casing,specially designed for slurry handling.The impeller has three blades.The ratio of the impeller diameter to the eye tip diameter is 2.49.PIV System.Figure 2shows the PIV setup.The PIV hard-ware consists of a 50mJ/pulse Nd:YAG laser ͑532nm wave-length ͒,laser light sheet optics,a charge coupled device ͑CCD ͒camera ͑Dantec®DoubleImage 700cross-correlation camera;resolution:768ϫ484pixels)equipped with a 60mm Micro Ni-kkor lens ͑Nikon ͒.The laser beam ͑3.5mm diameter ͒is formed into a light sheet ͑0.37mm thick;256mm wide ͒using a combi-nation of cylindrical and spherical lenses.The central 70mm of the light sheet width illuminates the plane of interest in the pump.The CCD camera is mounted on a 3-D traverse with a translation accuracy of Ϯ0.0254mm in each direction,and has its focal axis perpendicular to the plane of the laser light sheet to acquire flow images.A pair of single exposure image frames is required to enable cross-correlation data processing.The image pairs are pro-cessed into vector maps,in real-time,by the DantecFlowMap®PIV 2000processor.The image pair acquisition was synchronized to the impeller rotation using a once per revolution signal.An optical encoder located at the pump shaft generates a signal when the impeller blade reaches a desired location which then triggers the digital delay generator ͑DDG ͒.The DDG in turn sends a sig-nal to the PIV 2000processor,which then fires the laser and acquires the images from the PIV camera.The camera lens is operated at f/#8and the field of view ͑FOV ͒is 54ϫ39.9mm yielding an optical magnification of 0.165.The images were analyzed using a subregion size of 64ϫ64pixels with 50%overlap.This resulted in 23ϫ14vectors in the FOV with a spatial resolution of 2.25ϫ2.64mm/vector.Test set-up de-tails are provided in Charoenngam ͓21͔.Tests were conducted with sodium iodide as the working fluid,which matches the refractive index of the acrylic pump to obtain flow images without any optical distortion.The particles in sus-pension are spherical glass beads of 500micron size ͑density2.5Fig.1Centrifugal slurry pump with clear casing and clearimpellerFig.2The PIV setupg/cc ͒at a volumetric concentration of 2.5%and 5%Ϯ0.05%͑corresponding to 3.7%and 7.2%weight concentration ͒.At these concentration levels,particle interactions may not be ignored.Fig-ure 3shows the regions of interest in the casing and impeller of the centrifugal slurry pump selected for the investigation as well as the laser light sheet plane location.The light sheet is off the center plane by 1mm to avoid the joint between the two parts of the casing.The measurements were conducted at flow rates of 120͑725rpm ͒and 170͑1000rpm ͒gallons per minute.The corre-sponding Reynolds number ͑based on the impeller diameter and linear velocity of the impeller tip ͒were 3.1ϫ106and 4.3ϫ106,for these two flow rates.The frame grabber and the pump impeller were synchronized so that the images could be captured at a specific blade position.The effect of blade angular position is studied by acquiring the images at different blade angular positions varying from 0to 120degrees in increment of 5degrees.Figure 4shows the convention of the position of the blade ͑counter-clockwise ͒.Uncertainty in blade position was estimated to be 2%.Results and DiscussionThe velocity distribution for the particles and the fluctuation kinetic energy maps were obtained at three locations,which cover the discharge region ͑location 0͒,the tongue region ͑location 1-1͒and the impeller passage region ͑location 1-2͒.Measurements were obtained at particle volumetric concentrations of 2.5%and 5%.Five hundred image frame pairs were acquired for each op-erating condition.Cross-correlation processing and Chauvenet’s criterion were used to obtain the ensemble averaged velocity vec-tor maps for particle flow.The fluctuation kinetic energy was then calculated using the velocity vector data.The uncertainties in the measurements of velocity and kinetic energy fluctuation were de-termined to be Ϯ5.1%and Ϯ7.8%,respectively.Themeasure-Fig.3Locations of the field of view in the centrifugal slurry pump and the laser light sheetplaneFig.4Blade angularpositionFig.5Velocity field for particle flow ...00blade position ...,2.5percent volumetric concentration,...a ...725rpm....b (1000)rpmFig.6Fluctuation kinetic energy map …00blade position …,2.5percent volumetric concentration,…a …725rpm.…b …1000rpmment uncertainties reported are to a confidence level of 95%.They were computed by taking a quadrature of the random error and the full-scale bias error associated with the PIV measurements,further details of the uncertainty analysis are provided in Charoennegam͓21͔.The velocities obtained from this study are the velocity fields for the solid particle flow.The particle settling velocity is com-paratively high ͑9.62cm/s ͒as compared to normal 15m mean diameter seed particle ͑0.0086cm/s ͒.Because of the large inertia,the particles do not follow the flow verywell.Fig.7Streamlines and stagnation point for various operating conditions.…a …725rpm,2.5percent concentration,…b …1000rpm,2.5percent concentration,…c …725rpm,5percent concentration,…d …1000rpm,5percentconcentration.Fig.8Velocity field for particle flow ...00blade position ...,5per-cent volumetric concentration,...a ...725rpm....b (1000)rpm Fig.9Fluctuation kinetic energy map …00blade position …,5percent volumetric concentration,…a …725rpm.…b …1000rpmBlade Position at 0Degrees2.5%Volumetric Concentration.Figure 5shows the particle flow fields for 725rpm and 1000rpm pump speeds respectively,while the fluctuation kinetic energy map of the solid particles is provided in Fig.6.The fluctuation kinetic energy was determined by finding the mean of the square of the individual fluctuations at each subregion.Only the fluctuation kinetic energy in the x-direction is reported,since it is assumed to be isotropic.The flow is separated by the tongue into two streams and is similar for both pump speeds.The stagnation point occurs on the tongue.Location of the stagnation point virtually remains the same for both pump speeds as shown in Figs.7͑a ͒and 7͑b ͒.The large impact angle of the particles on the tongue may result in substan-tial erosion,since the material of an actual industrial pump would be brittle,and brittle materials have been found to be more sus-ceptible to erosion at high impingement angles ͓18͔.The particle velocities in the impeller passage area ͑between the passing and the up-coming blade ͒are high compared to that of the particles moving out to the discharge region.This suggests that the lower part of tongue region could wear out quicker relative to upper part of the tongue because of the frictional ͑cutting ͒wear due to solid particles,and the higher pump speed may result in greater wear as higher velocities are observed.The tangential component of the velocity vector is dominant in the discharge region resulting in a predominantly horizontal flow as the particles move out through the discharge region.The fluctuation kinetic energy of solid par-ticles relates to the random impingement wear mechanism.The contour plot in the fluctuation kinetic energy map is smaller than velocity vector field in the particle velocity map due to the inter-polation technique.As the pump speed increases to 1000rpm,the fluctuation kinetic energy increases approximately 300%.The highest fluctuation kinetic energy is observed at the lower part of tongue as well as the impeller passage area.This could imply that the lower part of tongue region may erode faster than other areas due to the random impingement particle mechanism.5%Volumetric Concentration.From Fig.8,it can be ob-served that the flow patterns for a 5%volumetric concentration are similar to those described for the 2.5%concentration case at both pump speeds.The difference is in the magnitudes of the particle velocities,which are slower for higher concentration due to supplementary inertial effects.Figures 7͑c ͒and 7͑d ͒show that the stagnation point still occurs on the tongue,and the change in location is insignificant with respect to speed and concentration.From Fig.9,it can be noted that the highest fluctuation kinetic energy was obtained at the lower part of the tongue and is higher than that for the 2.5%concentration,approximately 300%–450%.This may be attributed to higher particle interactions,which are possibly caused by higher local volumetric concentration.Local concentration becomes higher when the total concentration in-creases as shown by Charoenngam ͓21͔.The wearmechanismFig.10Velocity field for particle flow,725rpm,5percent volu-metric concentration,…a …500blade position,…b …600bladepositionFig.11Velocity field for particle flow,1000rpm,5percent volumetric concentration,…a …500blade position,…b …600blade positioncaused by directional and random impingement also occurs in the tongue region,similar to the 2.5%volumetric concentration case.Blade Positions at 50°and 60°.Because the velocity and the fluctuation kinetic energy of the solid particles at the lower region of the tongue were found to be higher than the other regions at a 0degree blade angle,further investigations were conducted in this specific region to examine the effects of blade angular position at a volume concentration of 5%.Figure 10shows the particle ve-locity fields for a pump speed of 725rpm at blade angular posi-tions of 50and 60degrees.Figure 11presents the particle veloci-ties for the same location at 1000rpm at identical blade angular positions.For both pump speeds,higher velocities were observed on the suction side of the blade as well as in the blade trailing edge region as the blade swept through the field of view.Unlike the discharge region ͑upper half ͒,the flow in the impeller passage ͑lower half ͒has substantial radial and tangential velocity compo-nents.The impact of particles on the tongue may cause erosion.On the pressure side of the blade,the frictional wear pattern can be caused by the particles that can not be maintained in the sus-pension and accumulate into sliding layers.Particle velocities on this side are slower than the blade tip velocity–e.g.,8.96m/s for 725rpm pump speed and 12.36m/s for 1000rpm pump speed.The directional impingement wear mechanism can also occur on the pressure side of the blade,resulting from the particles with velocities that are slower than the blade velocity.At pump speeds of 725rpm and 1000rpm,the high fluctuation kinetic energy was observed in the impeller passage area for all blade angular positions.Figures 12and 13show that at 725rpm,the fluctuation kinetic energy is very low compared to 1000rpm for all areas.The high fluctuation kinetic energy occurs on the suction side and at the lower area near the tongue.At 725rpm,the wear mechanism due to the random impingement of particles could occur on the suction side of the blade at 60°blade position as shown in Fig.12.However,Fig.13shows that at 1000rpm,the fluctuation kinetic energy in the impeller passage region and in the discharge region increases approximately 250%–500%on the pressure side and on the suction side of the blade,for all observed blade positions.This can result in greater wear on both sides of the blade.The erosion due to the random impingement on the suction side could be higher than other regions and may increase as the speed increases.The random impingement may result in a greater wear on the pressure side of the blade and at the lower region of the tongue when the speed increases from 725rpm to 1000rpm.From the data provided in Figs.10–13,it is possibletoFig.12Fluctuation kinetic energy map 725rpm,5percent volumetric concentration,…a …500blade position …b …600bladeposition Fig.13Fluctuation kinetic energy map 1000rpm,5percent volumetric concentration,…a …500blade position,…b …600blade positionestimating the velocityfluctuation magnitude from the ratio of the square root of the kinetic energyfluctuation and the mean veloc-ity.The maximum value occurred on the suction side of the blade and was determined to be0.37,showing significant velocityfluc-tuations in that region.ConclusionsThe particle image velocimetry technique was successfully uti-lized to investigate the velocities and kinetic energyfluctuations of slurry particles at the tongue region of an optically-clear cen-trifugal pump.The tongue region separates theflow into two streams where the location of the stagnation point on the tongue was not significantly affected by either the pump speed or the solid concentration in the ranges tested.In the impeller passage region,the highest velocities are generated on the suction side of the blade and in the blade trailing edge region as the blade sweeps through.However,these particle velocities are slower than the circumferential velocity of the blade tip͑8.96m/s for725rpm pump speed and12.36m/s for1000rpm pump speed͒.The tan-gential velocity component and the radial velocity component are significant in this region.In contrast,the particles that are moving through the discharge region are much slower and are nearly tan-gential͑horizontal͒.The up-coming blade does not appear to sub-stantially affect theflow velocity.Thefluctuation kinetic energy increased approximately200%to500%as the pump speed was increased from725rpm to1000rpm.The maximumfluctuation kinetic energy typically occurs on the suction side of the blade. The directional impingement mechanism is more significant at the pressure side of the blade,tongue and the casing.This mechanism becomes more important as the speed increases.This suggests that the impeller,tongue and the casing of the slurry pump can wear out quickly,especially with an increase in speed.The random impingement mechanism caused by thefluctuation kinetic energy of the solids can play an important role on the blade surface͑pres-sure side and suction side͒and the casing wall erosion.Frictional wear mechanisms can be caused by the particles that do not stay suspended in theflow and accumulate into sliding beds along the pressure side of the blade.PIV measurements in the slurry pump model can add significantly to the understanding of theflow through the pump.The information aids in the understanding of the wear mechanisms in such pumps and can be used for the design,modification,and calibration of computer codes for the development of long-life,efficient slurry pumps. AcknowledgmentsThe assistance of Mr.D.Conger of the Dept.of Mechanical and Aerospace Engineering,Case Western Reserve University,in making the experimental apparatus operational is greatly appreci-ated.The support provided by GIW Industries Inc.,Grovetown, Georgia,U.S.A.is gratefully acknowledged.The support of NASA Glenn Research Center and the Department of Mechanical and Aerospace Engineering at Case Western Reserve University is also acknowledged.References͓1͔Miner,S.M.,1988,‘‘Potential Flow Analysis of a Centrifugal Flow:Compari-son of Finite Element Calculation and Laser Velocimetry Measurement,’’Uni-versity of Virginia.University of Virginia Report No-UV A/643092/MAE88/ 369,Charlottesville,V A.͓2͔Liu,C.H.,Nouri,J.M.,Vafidis,C.,and Whitelaw,J.H.,1990,‘‘Experimental Study of Flow in a Centrifugal Pump,’’5th Intl.Symp.Application of Laser Techniques to Fluid Mechanics,Lisbon,Portugal.pp.114–129.͓3͔Liu,C.H.,Vafidis,C.,and Whitelaw,J.H.,1994,‘‘Two-Phase Velocity Dis-tributions and Overall Performance of a Centrifugal Slurry Pump,’’ASME J.Fluids Eng.,116͑2͒,pp.303–309.͓4͔Dong,R.,Chu,S.,and Katz,J.,1992,‘‘Quantitative Visualization of the Flow Within the V olute of a Centrifugal Pump.Part A:Technique,’’ASME J.Fluids Eng.,114͑3͒,pp.390–395.͓5͔Dong,R.,Chu,S.,and Katz,J.,1992,‘‘Quantitative Visualization of the Flow Within the V olute of a Centrifugal Pump.Part B:Results and Analysis,’’ASME J.Fluids Eng.,114͑3͒,pp.396–403.͓6͔Paone,N.,Riethmuller,M.L.,and Van den Braembussche,R.A.,1988,‘‘Ap-plication of Particle Image Displacement Velocimetry to a Centrifugal Pump,’’Proc.4th Intl.Symp.Applications of Laser Techniques to Fluid Mechanics, Lisbon,Portugal.͓7͔Oldenburg,M.,and Pap,E.,1996,‘‘Velocity Measurement in the Impeller and in the V olute of a Centrifugal Pump by Particle Image Velocimetry,’’Proc.8th Int.Symp.Applications of Laser Techniques to Fluid Mechanics,Lisbon,Por-tugal,pp.8.2.1–8.2.5.͓8͔Roco,M.C.,1993,‘‘Particulate Two-Phase Flow,’’Butterworth-Heinemann, Boston,Chapter10:Instrumentation.͓9͔Altobelli,S.A.,Givler,R.C.,and Fukushima,E.,1991,‘‘Velocity and Con-centration Measurements of Suspensions by Nuclear Magnetic Resonance Im-aging,’’J.Rheol.,35͑5͒,pp.721–772.͓10͔Roco,M.C.,and Addie,G.R.,1983,‘‘Analytical Model and Experimental Studies on Slurry Flow and Erosion in Pump Casings,’’Proc.8th Intl.Tech-nical Conf.on Slurry Tech.,Slurry Transport Association,Washington,DC,p.263.͓11͔Roco,M.C.,Addie,G.R.,Danis,J.,and Nair,P.1984,‘‘Modeling Erosion Wear in Centrifugal Pumps,’’Proc.9th Intl.Conf.Hydraulic Transport of Solids in Pipes,pp.291–316.͓12͔Roco,M.C.,Addie,G.R.,and Visintainer,R.,1985,‘‘Study on Casing Per-formances in Centrifugal Slurry Pumps,’’Part.Sci.Technol.,3,pp.65–88.͓13͔Roco,M.C.,Addie,G.R.,Visintainer,R.,and Ray,L.,1986,‘‘Optimum Wearing High Efficiency Design of Phosphate Slurry Pumps,’’Proc.11th Intl.Conf.Slurry Technology,Hemisphere,Washington,DC,pp.65–88.͓14͔Roco,M.C.,Marsh,M.,Addie,G.R.,and Maffett,J.R.,1986,‘‘Dredge Pump Performance Prediction,’’J.Pipelines,5͑3͒,pp.171–190.͓15͔Wilson,K.C.,1986,‘‘Effect of Solid Concentration on Deposit Velocity,’’J.Pipelines,5͑4͒,pp.251–257.͓16͔Wilson,K.C.,Addie,G.R.,and Clift,R.,1992,Slurry Transport Using Centrifugal Pumps,Elsevier,New York.͓17͔Wilson,K.C.,Addie,G.R.,Sellgren,A.,and Clift,R.,1997,Slurry Transport Using Centrifugal Pumps,2ed,Blackie Academic and Professional,London, UK.͓18͔Shook, C.,and Roco,M.,1991,Slurryflow:Principles and Practice, Butterworth-Heinemann,Boston.Chapter8:Wear in Slurry Equipment.͓19͔Addie,G.R.,1996,‘‘Slurry Pipeline Design for Operation with Centrifugal Pumps,’’Proc.13th Intl.Pump Users Symposium,College Station,TX,pp.193–211.͓20͔Cader,T.,Masbernet,O.,and Roco,M.C.,1994,‘‘Two-Phase Velocity Dis-tributions and Overall Performance of a Centrifugal Slurry Pump,’’ASME J.Fluids Engineering Conf.,Washington,DC,June20–24.116͑2͒,pp.176–186.͓21͔Charoennegam,P.,2001,‘‘Particle Image Velocimetry Investigations of a Slurry Flow in a Centrifugal Pump,’’M.S.Thesis,Case Western Reserve Uni-versity,Cleveland,Ohio.Dr.Jaikrishnan R.Kadambi is Professor of Mechanical and Aerospace Engineering at Case Western Reserve University,Cleve-land,Ohio.Prior to joining Case,he was a Senior Research Engineer in the Fluid Mechanics branch at the Westinghouse Research Laboratories,Pittsburgh,Pa from1971to1985.He received his Ph.D.in Mechanical Engineering from University of Pittsburgh.His primary areas of interest include turbomachinery,cardiovascular biofluid mechanics,multiphaseflow in porous media,laser basedflow diagnostic techniques(PIV,LDA)and geological sequestration of carbon dioxide.Mr.Pathom Charoenngam is a graduate student at Case Western Reserve University,Cleveland,Ohio.He completed his M.S. (Mechanical Engineering)degree in2001and is now pursuing his Ph.D.Dr.A.Subramanian received his Ph.D.from the Indian Institute of Science,India.He was Senior Research Associate and Manager of Laser Flow Diagnostics Laboratory in the Mechanical and Aerospace Engineering Department at Case Western Reserve University,Cleveland,Ohio from1998through2001.At present he is a Research Scientist at B.D.Biosciences Inc.,San Francisco,Ca.。
计量经济学中英文词汇对照
Controlled experiments Conventional depth Convolution Corrected factor Corrected mean Correction coefficient Correctness Correlation coefficient Correlation index Correspondence Counting Counts Covaห้องสมุดไป่ตู้iance Covariant Cox Regression Criteria for fitting Criteria of least squares Critical ratio Critical region Critical value
Asymmetric distribution Asymptotic bias Asymptotic efficiency Asymptotic variance Attributable risk Attribute data Attribution Autocorrelation Autocorrelation of residuals Average Average confidence interval length Average growth rate BBB Bar chart Bar graph Base period Bayes' theorem Bell-shaped curve Bernoulli distribution Best-trim estimator Bias Binary logistic regression Binomial distribution Bisquare Bivariate Correlate Bivariate normal distribution Bivariate normal population Biweight interval Biweight M-estimator Block BMDP(Biomedical computer programs) Boxplots Breakdown bound CCC Canonical correlation Caption Case-control study Categorical variable Catenary Cauchy distribution Cause-and-effect relationship Cell Censoring
质量单位的作文题目
质量单位的作文题目英文回答:Unit of Measurement.Measurement is an essential aspect of our daily lives. It helps us understand and quantify the world around us. One important aspect of measurement is the use of units. Units provide a standardized way of expressing quantities and allow for easy comparison and communication.There are various systems of units used around the world, such as the metric system, the imperial system, and the US customary system. Each system has its own set of units for measuring different quantities.In the metric system, the basic unit of length is the meter, while the basic unit of mass is the kilogram. Other commonly used metric units include the centimeter, millimeter, gram, and tonne. The metric system is widelyused in most countries and is known for its simplicity and ease of use.On the other hand, the imperial system, which is primarily used in the United States, uses units such as inches, feet, pounds, and ounces. The US customary system, which is a variation of the imperial system, is used for everyday measurements in the United States, such as cooking and construction.Units of measurement are also used in various fields, such as science, engineering, and medicine. For example, in physics, the unit of force is the newton, while in chemistry, the unit of volume is the liter. In medicine, units such as milligrams and milliliters are used to measure medication dosages.Units of measurement are important because they provide a common language for expressing quantities. They allow for accurate and precise communication, which is crucial in fields where precision is necessary. For example, in construction, using the wrong unit of measurement can leadto costly mistakes. Similarly, in scientific research, using inconsistent units can lead to inaccurate results.In addition to the standard units, there are also derived units, which are combinations of the base units. For example, the unit of speed is meters per second, which is derived from the base units of length and time. Derived units are used to express quantities that are derived from other quantities.In conclusion, units of measurement are essential for quantifying and understanding the world around us. They provide a standardized way of expressing quantities and allow for accurate communication. Whether it's measuring length, mass, time, or any other quantity, units help us make sense of the world.中文回答:质量单位。
面包海星皂苷-1对恶性胶质瘤细胞系U87MG增殖抑制的体外研究
面包海星皂苷-1对恶性胶质瘤细胞系U 87M G增殖抑制的体外研究程 光 张 赟 章 翔 汤海峰 费 舟 李 兵 曹卫东 高大宽 摘 要 目的:初步探讨面包海星皂苷-1(a sterosaponins 1)对体外恶性胶质细胞瘤细胞生长的影响及其作用机理。
方法:我们使用M T T 、细胞周期分析、Hoechst 33342细胞核染色荧光显微镜观察、透射电子显微镜观察等方法了解面包海星皂苷-1对U87M G 细胞的作用。
结果:实验结果显示,面包海星皂苷-1在很低的浓度(IC50= 4.3μg/mL )就够引起人胶质母细胞瘤细胞系U87M G 细胞的增殖抑制。
当面包海星皂苷-1作用浓度为3.4μg/mL 和4.3μg/mL 时,U87M G 细胞出现S 期阻滞,而其浓度为10.0μg/mL 时,U87M G 细胞出现G 0/G 1期阻滞。
同时,出现的亚二倍体峰(sub -G 1)随浓度和时间而增加。
Hoechst 33342细胞核染色荧光显微镜观察以及透射电子显微镜下细胞形态观察显示出典型的凋亡细胞形态学特征。
结论:体外实验初步显示,面包海星皂苷-1可明显抑制U87M G 细胞的增殖,并促其凋亡,具有显著的抗恶性胶质细胞瘤的药理作用。
关键词 胶质细胞瘤 面包海星皂苷-1 增殖抑制 凋亡中图分类号:R739.4 文献标识码:A 文章编号:1006-351X (2008)05-0575-04Inhibit i ng pr o lif era tio n of human glioblastoma U 87MG cells by Aster osa ponin 1CH E N G Guang ,Z HA N G Yun ,Z H A N G Xia ng ,et alDepa rtment of Neuro surgery of Xijing Hospital (Neuro sur ger y institute of P.L.A ),The Fourth Militar y Medical Universit y ,X i ’a n 710032,ChinaAbstract Objective :We did some prelimima ry resea rch in a novel chemothe rap y to malignant glioblastoma.Astero 2saponin 1,a new cytostatic a gent f ro m the starfish Culcita novaeguineae appear to exhibit va rious biological activitie s ,but t he f unction a nd mec hanism of t his new a gent o n glioblasto ma cells has n ot p reviously been dete rmined.Methods :In the pre sent study ,we inve stigated the prolife ration c hange of human glioblastoma U87M G cells exposur ed to diffe rent con 2cent rations of a sterosaponin 1for diffe rent time.Results :The re sult s showed t hat asterosaponin 1significantly suppre ssedU87M Gcell prolif eration in a time -and dose -dependent ma nner (IC50=4.3μg/mL ).Cell c ycle a nalysis of U87M Gcells showed that aste ro saponin 1induces the p rominent appeara nce of a sub -G1peak suggestive of apoptosis.Fur the r 2more ,U87M G cells treat me nt with asterosaponin 1re sulted in nuclea r condensation wit h apoptotic bodie s obse rved by b ot h fluore sce nce and elect ron microscopy.Conclusion :These novel findings suggest tha t the cyto static actions of astero 2saponin 1towar d U87M G cell s r esult f ro m the induction of cell apoptosis.Key w or ds :gliobla stoma Asterosaponin 1 Inhibiting prolif eration Apopto sis基金项目:国家自然科学基金资助项目(20502035)作者单位:710032西安第四军医大学西京医院神经外科(程光,章翔,费舟,李兵,曹卫东,高大宽),基础部免疫教研室(张赟),西京医院药剂科(汤海峰)通讯作者章翔 面包海星皂苷-1(a sterosaponi n 1)是我国科学家对我国南海三亚海域面包海星系统化学分析,分离鉴定的7种新的海星皂苷之一,研究表明它具有多种生物活性,包括溶血、抗病毒、抗肿瘤等,但其对脑胶质细胞瘤的作用及作用机理国内外尚无研究,我们利用人脑胶质母细胞瘤细胞系U87M G 研究它在体外抑制恶性胶质细胞瘤细胞生长中的作用,探索作用机理。
LeicaEMHPM100-LeicaMicrosystems
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No conventional freezing method can generate such large, well frozen samples.The unique 6 mm diameter carrier system of the Leica EM HPM100 allows even more sample area to be frozen, like no other high pressure freezing instrument.The state-of-the-art design of the Leica EM HPM100 enables ex-press sample handling and easy use with perfect freezing results.Inspired to Make the Difference3Leica Design by Werner HölblThe Unique Leica EM HPM100 Features• Automatic freezing of samples with one button operation • Extremely high cooling rates• Perfect freezing of samples up to 6 mm diameter • No cryoprotectants required• Rapid transfer loading device for CLEM work • Fast, ergonomic sample loading • Express sample handling for fast freezing • G raphical data display of actual temperature,time and pressure for each run • Integrated work station• Integrated stereomicroscope and adjustable LED illumination • Universal and application specifi c sample carriers • Integrated Dewar with drain outlet• Automatic sample transfer to LN 2 – no manipulation required • Automatic bake-out cycle• Thermally insulated process chamber• Integrated touch control screen with prompt window • Freezing process cover for ultimate safety • USB interface for data storage and transfer • Integrated air compressor • Compact and mobile• Uses standard 100–250 V power supplyThe Leica EM HPM100 is an automatic and easy to handle high pressure freezing instrument. With a one button operation, a cartridge holding the sample is inserted into the high pressure chamber.Simplicity with Technology4The vitrifi ed sample is automatically transferred to the LN 2-fi lled Dewar. The freezing process is digitally displayed on a graph showingthe actual temperature, time and pressure.Sample cartridgeThe sample cartridge is made of high performance insulating poly-mer and consists of three components: two cylinders with a fl ow channel, and a carrier plate with an opening to hold the sample carrier assembly.The sample cartridge functions as process chamber and sample holder at the same time.The integrated insulating fl ow channels direct the freezing onto the sample.This results in well frozen samples and low liquid nitrogen con-sumption.An air-driven intensifi er pressurizes the liquid nitrogen to 2100 bar. The process chamber is fi lled with a small volume of alcohol prior to freezing. The high pressure liquid nitrogen valve opens after the freezing pressure is reached. The pressurized liquid nitrogen is injected into the process chamber. The fl ow channels direct the rapid fl ow of liquid nitrogen along the sample carrier surfaces. This induces extremely high cooling rates.High cooling rates with the high-pressurized liquid nitrogen system1 LN2 storage 2 Feed pump3 Non return valve4 Air driven pressure intensifi er5 High pressure LN 2 valve6 Alcohol reservoir7 Alcohol dosing system8 Non return valve9 Sample cartridge 10 High pressure chamber 11 Pressure sensor 12 Temperature sensor 13 Exhaust 14 Locking pins15 Cartridge handling systemUpper cylinderCarrier plateLower cylinderFlow5➀➂Easy Freezing with the Leica EM HPM100➁One button operationWith a one button operation the enclosed sample is injected for freezing. The sample is automatically frozen in less than 2.5 seconds after injection.Complete. Convenient. Preparation.6ergonomically and conveniently prepare samples for freezing.The vitrifi ed sample is automatically released into a conveniently located LN 2-fi lled Dewar. The sample stays well frozen at all times.7Revolutionary in Application The Leica EM HPM100 offers a variety of sample carriers suit-able for almost any type of application. Most notable is the 6 mmdiameter sample carrier, which no other high pressure freezinginstrument offers.Once frozen, samples can be placed into the cryo chamber of theLeica EM UC6/FC6 ultramicrotome for frozen hydrated sectioningor the Leica EM AFS2 Automatic Freeze Substitution System. Incombination with freeze substitution, high pressure freezing isan excellent alternative to chemical fi xation for immunoelectronmicroscopy as the antigenicity and ultrastructure are both wellpreserved.89The 6 mm carrier opens up new perspectives for correlative mi-croscopy, as it allows a true pre-selection of a region of interest by CLSM within large areas and the EM investigation of the same sample without the drawback of artefacts caused by chemical fi xation.The New 6 mm Diameter Sample Carrier. Only by Leica Microsystems.High pressure frozen mouse skin biopsy (diameter 6 mm)after freeze substitution in uranyl acetate/acetone.3 mm 6 mm10High pressure frozen mouse skin b iopsy (diameter 6 mm) after freeze substitution in uranyl acetate/acetone solu-tion post-stained with uranyl acetate and lead citrate and sectioned prior to imaging in a TEM. Electron Microscopy ETH Zurich (EMEZ).Excellent Preservation of Ultra StructureHigh pressure frozen Nematode. Freeze substituted in water-free acetone containing 2% O s04, embedded in Epon/Araldite, thin sectioned and stained with uranyl acetate 2% and Reynolds lead citrate. Hexadecene was used as transmission fl uid. Electron Microscopy ETHZ urich (EMEZ). Specimen courtesy of M. Gotta, Instituteof Biochemistry, ETH Zurich, Switzerland.11Cryo SEM image of a high pressure frozen suspension of bakers yeast Saccharomyces cerevisiae. Freeze frac-tured in the Leica EM BAF060 at -115°C, etched at -105°C for 5 minutes, coated by electron beam evaporation with3 nm of Pt/C. Electron Microscopy ETH Zurich (EMEZ).Alternatively, when using the appropriate sample carriers, samples can be transferred to the Leica EM BAF060 Freeze Etch System and processed for high resolution cryo-SEM or to make high resolution replicas for TEM, amongst other applications.High pressure frozen Macrophage monolayer grown on Sapphire disc. Freeze substituted in water-free acetone containing 2% O s04, embedded in Epon/Araldite, thin-sectioned and stained with uranyl acetate 2% and R eynolds lead citrate. Electron Microscopy ETH Zurich (EMEZ). Specimen courtesy of G. Sumara, Institute of Cell Biology, ETH Zurich, Switzerland.Leica Microsystems operates internationally in four divi-sions, where we rank with the market leaders.• Life Science DivisionThe Leica Microsystems Life Science Division supports the imaging needs of the scientifi c community with advanced innovation and technical expertise for the visualization, measurement, and analysis of microstructures. Our strong focus on understanding scientifi c applications puts Leica Microsystems’ customers at the leading edge of science.• Industry DivisionThe Leica Microsystems Industry Division’s focus is to support customers’ pursuit of the highest quality end result. 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Milli-Q水质仪产品说明书
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神奇的纳米和纳米技术
神奇的纳米和纳米技术
韩王荣
【期刊名称】《上海科学生活》
【年(卷),期】2001(000)007
【摘要】“纳米”是英文nanometer的译名,它是一种度量单位,1纳米为百万分之一毫米,即1毫微米,也就是10亿分之一米。
纳米结构通常是指尺寸在100纳米以下的微小结构。
1982年,扫描隧道显微镜发明以后,便诞生了一门以0.1至100纳米长度为研究对象的技术一一纳米技术,它以前所未有的分辨率,为人类揭示了一个可见的原子和分子的世界,它的最终目标,是直接以原子和分子来构造具有特定功能的产品,也就是以分子和原子为加工对象,制造出微型机器。
【总页数】5页(P17-21)
【作者】韩王荣
【作者单位】无
【正文语种】中文
【中图分类】G633
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2.神奇的纳米技术 [J], 贾平
3.神奇的纳米技术 [J], 叶玮玮
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5.神奇的DNA纳米技术 [J], 樊春海;李江
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a r X i v :a s t r o -p h /0611844v 1 28 N o v 2006Astronomy &Astrophysics manuscript no.6383cESO 2008February 5,2008The millimetre variability of M81*Multi-epoch dual frequency mm-observations of the nucleus of M81R.Sch¨o del 1,M.Krips 2,S.Marko ff3,R.Neri 4,and A.Eckart 11I.Physikalisches Institut,Universit¨a t zu K¨o ln,Z¨u lpicher Str.77,D-50937K¨o ln,Germanye-mail:rainer@ph1.uni-koeln.de,eckart@ph1.uni-koeln.de2Harvard-Smithsonian Center for Astrophysics,SMA project,645North A’ohoku Place,Hilo,HI 96720e-mail:mkrips@3Sterrenkundig Instituut “Anton Pannekoek ”,Universiteit van Amsterdam,Kruislaan 403,1098SJ Amsterdam,The Netherlands e-mail:sera@science.uva.nl4Institut de Radio Astronomie Millim´e trique,300rue de la Piscine,Domaine Universitaire,38406Saint Martin d’H`e res,France e-mail:neri@iram.frReceived September ;acceptedABSTRACTAims.There are still many open questions as to the physical mechanisms at work in Low Luminosity AGN that accrete in the extreme sub-Eddington regime.Simultaneous multi-wavelength studies have been very successful in constraining the properties of Sgr A*,the extremely sub-Eddington black hole at the centre of our Milky Way.M81*,the nucleus of the nearby spiral galaxy M81,is an ideal source to extend the insights obtained on Sgr A*toward higher luminosity AGN.Here we present observations at 3and 1mm that were obtained within the framework of a coordinated,multi-wavelength campaign on M81*.Methods.The continuum emission from M81*was observed during three epochs with the IRAM Plateau de Bure Interferometer simultaneously at wavelengths of 3and 1mm.Results.We present the first flux measurements of M81*at wavelengths around 1mm.We find that M81*is a continuously variable source with the higher variability observed at the shorter wavelength.Also,the variability at 3and 1mm appears to be correlated.Like Sgr A*,M81*appears to display the strongest flux density and variability in the mm-to-submm regime.There remains still some ambiguity concerning the exact location of the turnover frequency from optically thick to optically thin emission.The observed variability time scales point to an upper size limit of the emitting region of the order 25Schwarzschild radii.Conclusions.The data show that M81*is indeed a system with very similar physical properties to Sgr A*and an ideal bridge toward high luminosity AGN.The data obtained clearly demonstrate the usefulness and,above all,the necessity of simultaneous multi-wavelength observations of LLAGN.Key words.LLAGN –mm-observations –galactic nucleus1.IntroductionAt high accretion rates onto black holes,the infall of gas and dissipation of energy via mostly thermal processes in a thin disk are fairly well understood in general terms.In con-trast,there is still great debate about which physical mech-anisms are dominant in objects which are accreting at well below the Eddington rate.The most extremely sub-Eddington source currently accessible to observations that allows still rea-sonable statistics for modelling the accretion /emission mech-anisms is the central supermassive black hole of our Galaxy,Sagittarius A*(Sgr A*).Its low luminosity has perplexed theo-rists for decades,and stimulated the re-emergence of radiatively ine fficient accretion flow models (RIAFS,see Melia &Falcke 2001;Quataert 2003),including the advection dominated ac-cretion flow model (ADAF,e.g.Narayan &Yi 1994)and its derivatives.These models are characterised by low e fficiency in converting thermal energy into electromagnetic radiation,but di ffer in terms of the inner boundary conditions such as the presence of strong winds (e.g.Blandford &Begelman 1999)and /or convection (Quataert &Gruzinov 1999),eachof2R.Sch¨o del et al.:The millimetre variability of M81* as contenders.Recently,Eckart et al.(2004,2006)were suc-cessful in obtaining thefirst simultaneous measurements of theemission from Sgr A*at X-ray/NIR wavelengths(with quasi-simultaneous sub-mm observations).These and ongoing coordi-nated multi-wavelengths campaigns deliver the decisive obser-vations for constraining/eliminating weak accretion models fur-ther.The nearby spiral galaxy M81(NGC3031)is an Sb spiralgalaxy similar to the Milky Way.It is located at a distance of3.63±0.34Mpc(Freedman et al.1994).Devereux et al.(2003)used spectroscopic measurements of the Hα+[N II]emission,probably emitted from a rotating gas disk inclined at an angle of14◦±2◦,to infer a mass of7.0+2−1×107M⊙for the central blackhole in M81.The nucleus of M81,termed M81*,shows typical signs of AGN activity.It has a power-law,variable X-ray continuum (Ishisaki et al.1996;Page et al.2004).The X-rayflux from M81 is highly variable,at scales from days to years(La Parola et al. 2004).The nucleus of M81displays double peaked,broad Hαemission lines(Bower et al.1996).However,the overall lumi-nosity and AGN characteristics of M81*are rather weak and the galaxy is classified as a LINER(low-ionisation nuclear emission-line region,e.g.Ho et al.1996).With a luminosity of the order of1037erg s−1in the radio and1040erg s−1in the optical/X-ray domains(see,e.g.,compilations by Ho et al.1996; Ho1999),its luminosity is<10−5times the Eddington luminos-ity in any wavelength regime.M81*is therefore counted among the low-luminosity AGN(LLAGN).It shows the typical spectral energy distribution(SED)of this class of sources,that is char-acterised by the absence of the so-called big blue bump,the ul-traviolet excess found commonly in the higher power AGN(e.g. Ho1999).At cm-wavelengths,M81*shows large-amplitude variations (factors up to two at2cm)with timescales of a few months and weaker changes of theflux density on timescales≤1day (Ho et al.1999).Multi-epoch VLBI observations of M81*at ∼0.01pc resolution at20epochs over4.5yr reveal a stationary core with a variable(on timescales of∼1yr)one-sided jet of length1mas(3600AU)towards the northeast(Bietenholz et al. 2000).As for the polarisation properties of M81*,circular po-larisation was detected at4.8,8.4(Brunthaler et al.2001),and 15GHz(Brunthaler et al.2006),while linear polarisation ap-pears to be absent at these frequencies.This is an intriguing sim-ilarity to Sgr A*,where circular polarisation also dominates over linear polarisation at these wavelengths(Bower et al.1999a,b,c; Aitken et al.2000;Bower et al.2003,2005).Sakamoto et al.(2001)present observations of the central kiloparsec of M81at a wavelength of3mm in the CO J=1−0 line and continuum at100pc resolution.They detect molecular gas in a pseudoring or spiral arm at about500pc,but no giant molecular cloud within about300pc of the nucleus.Theyfind significant intraday variation of the continuum emission from M81*,suggesting an emitting region of∼100AU.Reuter&Lesch(1996)obtained a spectrum of M81*from the radio to the mm-regime.Theyfind an inverted spectrum up to100GHz.Itsflux density can be described well by the law Sν∝ν1/3exp−(ν/νc)with a turnover frequency ofνc= 200GHz.They point out the remarkable similarity between the spectrum of M81*and Sgr A*and conclude that the same physi-cal mechanisms might operate in both galactic nuclei.Therefore Reuter&Lesch(1996)suggest that M81*may be a by∼104up-scaled version of Sgr A*.Thus M81*is a unique source for comparison with Sgr A* and more powerful AGN,and constitutes the next logical step after the successful multi-wavelength observations of Sgr A*. With this aim,a coordinated,multi-wavelength campaign took place in thefirst half of2005,involving instruments from the X-ray to the radio domain:the Chandra X-ray observatory,the Lick telescope(NIR),the SMA,the Plateau de Bure Interferometer (PdBI),the VLA,and the GMRT.A compilation of all observa-tions and the interpretation of the multi-wavelength data is pre-sented by Markoffet al.(in preparation).A detailed description of all instruments and the related data reduction would overload the multi-wavelength paper.Therefore,some papers are dedi-cated to the observations with specific instruments.The X-ray emission lines measured in the Chandra observations are dis-cussed by Young et al.(in preparation).In this paper we focus on the three epochs of mm-observations of M81*that were ob-tained with the PdBI during this campaign.2.Observations and data reductionM81*was observed with the PdBI on24February,14-15July, and19-20July2005.Six antennae were used for the February observations in the B configuration of the PdBI,which provides typical beam sizes around1.5′′at∼100GHz and around0.8′′at ∼200GHz.The July observations were done withfive antennae in the more compact D configuration,with typical beam sizes around5′′at∼100GHz and2.5′′at∼200GHz.All observations are listed in Table1.The observations aimed to detect continuum emission from M81*.However,due to good conditions the receivers were tuned to the12CO J=1−0and J=2−1transitions at115.3and 230.5GHz in the February observations.Thus,it was possible to search for compact CO-emission within∼20′′of the nucleus, while the continuum could be extracted from the line-free chan-nels(The results of the CO-line imaging will be discussed in a forthcoming paper).For greater phase stability in the July obser-vations,an epoch during which the atmosphere at the site con-tains more water vapour,the receivers were tuned to frequencies of80.5and86.2GHz at3mm and241.4and218.2GHz at1mm.The sources3C273(February),3C454.3(July14-15)and 1741-038(July19-20)were used for bandpass calibration. Phase calibration was performed with the sources1044+719and 0836+710.Primaryflux calibrators to determine the efficiencies of the antennae were the sources1044+719for February23-24(1.6Jy at3mm/1.1Jy at1mm),1044+719(1.8Jy at3mm) and2200+420(8.7Jy at1mm)for July14/15,and MWC349 (1.0Jy at3mm)and3C454.3(33.0Jy at1mm)for July20.The phase calibrators1044+719and0836+710were used tofit the time-dependentfluctuations of the amplitude for all baselines. Various tests were performed for estimating the uncertainty of theflux calibration,ing different primaryflux calibrators and comparing the resultingfluxes of all observed sources.As an additional test,from a comparison of the calibratedfluxes of the sources0836+710,1044+719,and MWC349between the three epochs we estimated the uncertainty of the absoluteflux calibra-tion.To provide a quantitative measurement of the data quality, Table2lists the ranges of the rms values of the phase and am-plitude that were obtained during calibration of these values for the different baselines for each observing epoch.The table also provides values for the estimated uncertainty of the absoluteflux calibration at3mm and1mm.As can be seen,the quality of the February data is highest.The data from20July are clearly better than the data from14/15July.Individual scans of20min duration were extracted from the calibrated data.Subsequently,a point source wasfitted to the re-sulting UV tables in order to determine theflux of M81*and ofR.Sch¨o del et al.:The millimetre variability of M81*3 Table1.Observations of M81*with the PdBI during2005.ν3mm is the exact frequency used around3mm,ν1mm the one at a wavelength of1mm.N Ant is the number of antennae,“Config”refers to the antenna configuration used.Start[UT]End[UT]ν3mm[GHz]ν1mm[GHz]N Ant ConfigTable2.Data quality:The table lists the ranges of the rms values of the phase and amplitude between all baselines for each observing epoch and wavelength.Also,the estimated systematic error of the absoluteflux calibration is indicated.3mm1mm1mmphase rms[deg]absolute absolute10-20≤10%≤15% 14/15-JUL-20058-1140-6025-3012-28≤15%≤20%4R.Sch¨o del et al.:The millimetre variability ofM81*Fig.1.Light curves of M81*and of the calibrators 1044+719and 0836+710from the PdBI observations at 3and 1mm.The x-axes show UT in hours.The fluxes were all scaled to an average value of 1,with the curves of the calibrators shifted for bettercomparison.The vertical lines in the top panels indicate sections for which the average flux and the standard deviation of the individual measurements have been calculated.The corresponding values are indicated.a sinusoidal pattern due to polarisation were observed at 3mm,one would expect to observe a similar pattern at 1mm,shifted by several hours.We do not detect the expected sinusoidal pattern in the light curves nor are the light patterns shifted by several hours in time relative to each other.b)Recent observations of M81*with the BIMA array by Brunthaler et al.(2006)indicateabsence of or very low upper limits of a few percent on linear and circular polarisation of M81*at 86and 230GHz.R.Sch¨o del et al.:The millimetre variability of M81*5 Fig.2.Light curves of M81*and of the calibrators1044+719and0836+710from the PdBI observations at3and1mm on February 24.The light curves were corrected for possible systematic variations by forcing the calibrator sources to have constantflux(onaverage).Table3.Averageflux densities of M81*,S3mm and S1mm,measured atν3mm andν1mm during the three epochs of the campaign. Themean and standard deviation(not the error of the mean)were calculated from the unweighted individual measurements shown in Fig.3.The last column lists the average spectral indices and their standard deviation(not the error of the mean)calculated from all individual measurements as shown in the right panels of Fig.4.Start[UT]End[UT]ν3mm[GHz]S3mmν1mm[GHz]S1mmαFig.3.Flux density of M81*on24February,14/15July,and20July2005.The black boxes mark theflux density at3mm and the green(gray)crosses mark theflux density at1mm.The exact frequencies are indicated in the titles of the individual panels.The error bars indicate relative,not absolute,uncertainties.4.DiscussionTheory and observations indicate that sub-Eddington black holesare jet-dominated(Falcke&Markoff2000;Falcke et al.2004;Yuan et al.2002;Fender et al.2003).A compact,variable jet hasin fact been detected in VLBI observations of M81*(Bietenholzet al.,2000;see also Markoffet al.,in preparation).Falcke(1996)demonstrate that a compact jet can explain the observedspectrum of M81*very well.In the jet model,the turnover fre-quency from aflat(or slightly inverted)radio spectrum to anoptically thin power-law occurs at a frequencyνt.In a simplifiedmodel this frequency depends on the jet power,Q j,and mass ofthe black hole,M BH,asνt∝Q2/3jM−1BH(Falcke et al.2004).InLLAGN the turnover occurs generally at(sub)mm wavelengths,while it is located in the near-infrared/optical regime for XRBsin the low/hard state(see Corbel&Fender2002;Markoffet al.2003;Falcke et al.2004).The simultaneous observations of M81*at3and1mm pre-sented in this work are consistent with a turnover of the syn-chrotron emission from a jet in M81*into the optically thinpart between3mm and1mm(with the exception of thefirst∼4hours of the24February light curve that would indicate ahigher turnover frequency).This is in good agreement with thedata presented by Reuter&Lesch(1996)that were,however,notacquired simultaneously.6R.Sch¨o del et al.:The millimetre variability ofM81*Fig.4.Spectral index of M81*on24February,14/15July,and20July2005.The spectral index is defined by Sν∝να,where Sνis theflux density at a given frequencyν.The absolute uncertainties(see Tab.2)of theflux densities at3and1mm have been taken into account for this plot.Since we use upper limits on the absolute uncertainties,the plotted error bars are conservative.The evidence for the location of the turnover frequency be-tween3and1mm is ambiguous,however.The multi-frequency radio data from the coordinated campaign indicate that bumps are present in the radio spectrum(Markoffet al.,in prep.).These bumps are located at different frequencies at different epochs. Therefore,in spite of the presented mm-data,it may well be pos-sible that there is a submm-bump present in M81*.Observations with the SMA at345Ghz that were obtained during the coordi-nated campaign on M81*indicate that theflux density of M81* increases toward the submm regime,in agreement with theo-retical predictions on the existence of a submm-bump(Markoffet al.,in preparation).Unfortunately,there was only one epoch (24February2005),where measurements with the PdBI and the SMA were simultaneous.Forfine-tuning the models of the emission of M81*it is im-portant to know the exact turnover frequency and whether and how it varies with activity of the source.Therefore there is an urgent need for more observational data for comparison with theory in order to understand sub-Eddington accretion and emis-sion.It is essential that the observations across the wavelength regimes are simultaneous.Due to the gap of several months there is probably no corre-lation between the February and July observations because the light curves at2cm presented by Ho et al.(1999)indicate that radio outbursts of M81*have generally timescales<5months. Also,Sakamoto et al.(2001)have found that M81*showsflux variations of factors≤2at a wavelength of3mm on time scales <10days.However,the decaying light curve from July19may be related to the same event as the light curve on July14.The spectral index during the two epochs is negative and of similar absolute value(see Tab.3and Fig.4).This behaviour is con-sistent with a decaying light curve from a low-peakingflare in the model by Valtaoja et al.(1992)that describes variability phe-nomena in AGN by a growth/decay of shocks in a jet(see also Marscher&Gear1985).An estimate of the size scales of the relevant processes can be obtained from the time scales of the observed variability.The drop-offbetween07:00UT and12:00UT in the highest quality data set from24February2006shows that a 5σvariability of theflux at3mm occurs on a time scale of5hours(see also the intraday variability at3mm found by Sakamoto et al.2001).A similarly rapid change of theflux can be seen at1mm.Since no signal can travel faster than at the speed of light this cor-responds to an upper limit on the size of the source of merely ∼25Schwarzschild radii,when a mass of7.0×107M⊙is as-sumed for the black hole in M81*.5.SummaryWe present three epochs of simultaneous1and3mm contin-uum observations of the LLAGN M81*that were obtained in the framework of a coordinated,multi-wavelength campaign (Markoffet al.,in preparation).The observations of M81*with the PdBI at mm-wavelengths confirm that M81*is a continuously varying radio contin-uum source as has been found previously,mostly at larger wavelengths(see Ho et al.1999;Bietenholz et al.2000; Sakamoto et al.2001).The measurements present thefirst un-ambiguous detection of M81*at1mm and moreover show that the source is continuously variable at this wavelength as well. The amplitude of the variability is observed to be generally larger at1mm than at3mm by a factor of roughly1.5.This agrees well with the trend found by Ho et al.(1999)that the am-plitude of the variability increases with frequency in LLAGN.A similar behaviour is found in the far weaker source Sgr A*(e.g. Herrnstein et al.2004;Miyazaki et al.2004).The shortest variability time scales of our observations give upper limits on the size of the emitting region of ∼25Schwarzschild radii,assuming a black hole mass of7.0×107M⊙.The decaying light curves observed on14/15and on20July 2005may have been related to the same radio outburst.They are consistent with a generalized shock-in jet model(Valtaoja et al. 1992).The simultaneous measurements of M81*at3and1mm are consistent with a turnover of theflux in the mm-to-submm regime as predicted by models for a jet dominated source.Some ambiguity remains concerning the exact peak frequency and the related interpretation of variability events.The observations confirm previousfindings that there are many similarities between M81*and Sgr A*,the source re-lated to the supermassive black hole at the center of the Milky Way.This underlines the similarity between LLAGN despite of several orders of magnitudes of difference between their lumi-nosities.M81*can serve as a bridge from the extremely sub-Eddington Sgr A*toward higher luminosity LLAGN.The radio emission from M81*is apparently dominated by a compact jet. 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