【WO2019217843A1】用于大麻微繁殖的系统、方法和试剂盒【专利】

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(51)International Patent Classification:
A01H 4/00(2006.01)A01H 6/28(2018.01)
Declarations under Rule 4.17:A01H 5/02(2018.01)
—as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(H))(21)International Application Number:
—as to the applicant's entitlement to claim the priority o f the PCT/US2019/031759
earlier application (Rule 4.17(iii))—o f inventorship (Rule 4.17(iv))(22)International Filing Date:
10May 2019(10.05.2019)Published:
—with international search report (Art.21(3))(25)Filing Language:
English (26)Publication Language:
English (30)Priority Data:
62/670,19811May 2018(11.05.2018)
US (71)Applicant:FRONT RANGE BIOSCIENCES,INC.
[US/US];1224Commerce Ct.#2,Lafayette,Colorado
80026(US).
(72)Inventors:ZAPATA CARRERO,Carmen Cecilia;774
South Lafayette Dr.#173,Lafayette,Colorado 80026(US).
SARMIENTO,Carolina;2600South Rock Creek Park¬
way #43-202,Superior,Colorado 80027(US).VAUGHT,
Jonathan David;5335N.113th St,Longmont,Colorado
80504(US).
(74)Agent:RYBAK,Sheree Lynn;Klarquist Sparkman,LLP,
One World Trade Center,Suite 1600,121SW Salmon
Street,Portland,Oregon 97204(US).
(81)Designated States (unless otherwise indicated,for every
kind o f national protection available):AE,AG,AL,AM,
AO,AT,AU,AZ,BA,BB,BG,BH,BN,BR,BW,BY,BZ,
CA,CH,CL,CN,CO,CR,CU,CZ,DE,DJ,DK,DM,DO,
DZ,EC,EE,EG,ES,FI,GB,GD,GE,GH,GM,GT,HN,
HR,HU,ID,IL,IN,IR,IS,JO,JP,KE,KG,KH,KN,KP,
KR,KW,KZ,LA,LC,LK,LR,LS,LU,LY,MA,MD,ME,
MG,MK,MN,MW,MX,MY,MZ,NA,NG,NI,NO,NZ,
OM,PA,PE,PG,PH,PL,PT,QA,RO,RS,RU,RW,SA,
SC,SD,SE,SG,SK,SL,SM,ST,SV,SY,TH,TJ,TM,TN,
TR,TT,TZ,UA,UG,US,UZ,VC,VN,ZA,ZM,ZW.
(84)Designated States (unless otherwise indicated,for every
kind o f regional protection available):ARIPO (BW,GH,
GM,KE,LR,LS,MW,MZ,NA,RW,SD,SL,ST,SZ,TZ,
UG,ZM,ZW),Eurasian (AM,AZ,BY,KG,KZ,RU,TJ,
TM),European (AL,AT,BE,BG,CH,CY,CZ,DE,DK,
EE,ES,FI,FR,GB,GR,HR,HU,IE,IS,IT,LT,LU,LV,
MC,MK,MT,NL,NO,PL,PT,RO,RS,SE,SI,SK,SM,
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KM,ML,MR,NE,SN,TD,TG).
(54)Title:SYSTEMS,METHODS AND KITS FOR MICROPROPAGATION OF CANNABIS
(57)Abstract:Provided herein are systems,methods and kits for micropropagating disease-free cannabis plants,referred to as the Clean Stock®method.Also provided are Clean Stock®cannabis plants produced using such systems,methods and kits.
SYSTEMS,METHODS AND KITS FOR MICROPROPAGATION OF CANNABIS
[0001]This application claims priority from US provisional application No.62/670,198,filed May11,2018,herein incorporated by reference in its entirety.
FIELD
[0002]The disclosure relates to systems,methods,and kits for tissue culture production of disease-free cannabis plants.
BACKGROUND
[0003]Cannabis is a genus of flowering plants that includes at least three species,Cannabis sativa, Cannabis indica,and Cannabis ruderalis,as determined by plant phenotypes and secondary metabolite profiles(chemotype).Both marijuana and hemp plants are in this genus and produce a unique family of terpeno-phenolic compounds called cannabinoids.The cannabinoids typically produced in greatest abundance are cannabidiol(CBD)and A-tetrahydrocannabinol(THC).CBD and THC have been shown to have different physiological effects when ingested.Cannabis is used to produce hemp fiber and hemp oil,for medicinal purposes,and as a recreational drug.Hemp cultivars of Cannabis are bred to produce minimal levels of THC,while marijuana cultivars are bred to produce higher levels of THC.CBD has been shown to have a number of medically useful effects such as anti-inflammatory,anti-convulsant,antioxidant,antiemetic,anxiolytic and antipsychotic effects,and THC is psychoactive.In general,the maximum THC content of hemp is0.3%and any cannabis with a THC content of greater than0.3%is considered to be marijuana.
[0004]Given these differences between hemp and marijuana,cannabis consumers want to know the relative levels of THC and CBD in the cannabis they are purchasing and consuming and are therefore interested in plant products that have a known and consistent phenotype and chemotype.In addition, cannabis consumers want to know that a hemp or marijuana product does not contain pesticides. Cannabis growers need to be able to provide consistent disease-free hemp and marijuana plants to consumers and to be able to carry out large scale cost-effective propagation of such plants.
[0005]Cannabis growers desire to select hemp and marijuana plants with a favorable physiological “phenotype”and chemical profile“chemotype”.In general,growers use vegetative propagation(or vegetative cloning)to multiply plants starting with a“mother”plant by way of’’cuttings”,using a small branch of the mother plant and rooting the branch to produce new“daughter”plants.。