细胞饥饿及 TNF-α干预后内皮细胞膜微粒对人脑微血管内皮细胞的影响

细胞饥饿及 TNF-α干预后内皮细胞膜微粒对人脑微血管内皮
细胞的影响
潘群文;何彩霞;刘雅静;张惠婷;王艳;戴炳琰;马晓瑭
【期刊名称】《山东医药》
【年(卷),期】2015(000)026
【摘要】目的：探讨饥饿及TNF-α刺激条件下，内皮细胞膜微粒（ EMVs ）对
人脑微血管内皮细胞（ HBMECs ）增殖、迁移及血管生成功能的影响。

方法体外培养HBMECs，分为PBS组、饥饿组、TNF-α组。

于饥饿24 h后提取饥饿后产
生的EMVs（ sHB-MVs），于TNF-α刺激24 h后提取TNF-α刺激下产生的EMVs（αHB-MVs）。

将sHB-MVs与αHB-MVs按照1×108个／mL、每孔
10μL分别与饥饿组及TNF-α组HBMECs共培养，PBS组给予10μL PBS处理。

采用MTT法测定各组HBMECs增殖能力，倒置显微镜下测定HBMECs迁移距离，观察血管形成数。

结果饥饿组增殖能力高于PBS组（P＜0．01），TNF-α组HBMECs增殖能力低于PBS组（P＜0．01）。

饥饿组迁移距离长于PBS组（P＜0．05），TNF-α组迁移距离短于PBS组（P＜0．01）。

饥饿组血管生成数多于PBS组（P＜0．01），TNF-α组血管生成数少于PBS组（P＜0．01）。

结论饥
饿刺激下产生的EMVs可促进HBMECs增殖、迁移及血管生成功能，TNF-α刺激下产生的EMVs可抑制HBMECs增殖、迁移及血管生成功能。

%Objective To investigate the effects of cell starvation and TNF-αderived endothelial microvesicles (EMVs) on the proliferation, migration and angiogenesis of human brain microvascular endothelial cells (HBMECs) in vitro.Methods HBMECs were cultured and were divided into the PBS group, starvation
group and TNF-αgroup.EMVs were extracted from HB-MECs cultured in a serum deprivation (SD) medium (starving stress, sHB-MVs) or SD medium containing tumor necrosis fac-tor-(TNF-α) (apoptotic stress,αHB-MVs).The HBMECs of the starvation group and TNF-αgroup were cultured with sHB-MVs andαHB-MVs (1 ×108/mL, 10μL each hole), respectively;and the PBS gr oup was treated with 10μL PBS.The prolif-eration of HBMECs in each group was determined by MTT, the migration distance of HBMECs was measured by inverted micro-scope and the number of angiogenesis was observed under the inverted microscope.Results The proliferation of HBMECs of the starvation group was higher than the PBS group, and TNF-αgroup was lower than that of the PBS group (all P<0.01).The migration distance of HBMECs in the starvation group was longer than that of the PBS group (P<0.05), and the TNF-αgroup was shorter than the PBS group (P<0.01).The number of angiogenesis of the starvation group was more than that of the PBS group (P<0.01),and the TNF-αgroup was less than the PBS group (all P<0.01).Conclusion The cell starvation-derived EMVs can promote the proliferation, migration and angiogenesis of HBMECs, but TNF-α-derived EMVs can inhibit the prolifer-ation, migration and angiogenesis of HBMECs.
【总页数】3页(P22-24)
【作者】潘群文;何彩霞;刘雅静;张惠婷;王艳;戴炳琰;马晓瑭
【作者单位】广东医学院附属医院神经病学研究所，广东湛江524001;广东医学院附属医院神经病学研究所，广东湛江524001;广东医学院附属医院神经病学研
究所，广东湛江524001;广东医学院附属医院神经病学研究所，广东湛江524001;广东医学院附属医院神经病学研究所，广东湛江524001;广东医学院附属医院神经病学研究所，广东湛江524001;广东医学院附属医院神经病学研究所，广东湛江524001
【正文语种】中文
【中图分类】R743.1
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