NKG2 D／RAE-1通路在介导 NK／T 细胞杀伤垂体瘤细胞中的作用及意义

NKG2 D／RAE-1通路在介导 NK／T 细胞杀伤垂体瘤细胞
中的作用及意义
孙其凯;马立新;李珍珠;陈正;李泽福
【期刊名称】《山东医药》
【年(卷),期】2016(056)044
【摘要】Objective To observe the effect of NKG2D/RAE-1 pathway on mediating natural killer ( NK) cell and CD8 +T cell (both cells were referred to as NK/T cells) killing hypophysoma cells and to investigate the immune escape mechanism of hypophysoma from the cytological level .Methods The hypophysoma cell line AtT 20 was selected .The im-munofluorescent labeling method was used to detect the expression level of NKG 2D ligand RAE-1, its position and distribu-tion in the hypophysoma cells .NK/T cells were isolated by NK/T cell sorting kit , and NK/T cells were placed in the upper chamber of the Transwell chamber , the target cells AtT20 were placed in the lower chamber of the Transwell chamber and then were randomly divided into the control group and experimental group .The control group was co-cultured under normal conditions for 0, 4, 8, and 24 h.In the experimental group, the anti-RAE1 antibody was added to the culture medium , and the cells were co-cultured for 0, 4, 8, and 24 h.MTT assay was used to detect the inhibitory rate of cell proliferation at each time point .Results RAE-1 was clearly expressed in AtT 20 cell membrane , and there was a weak expression in the cytoplasm .The positive expression
rate of RAE-1 protein in AtT20 cells was 100%, among which the strong expression rate was 30%(15/50), the moderate expression rate was 60%, the weak expression rate was 10%, and there was no neg-ative expression.In the control group, at 4, 8 and 24 h after culture, NK/T cell proliferation inhibition rate increased as compared with that at 0 h (all
P<0.05).In the experimental group, the proliferation inhibition rate gradually increased at 0, 4, 8 and 24 h (all P<0.05), and at 8 and 24 h, the proliferation inhibition rates in the experimental group were sig-nificantly higher than those of the control group (all P<0.05).Conclusions NK/T cells kill the hypophysoma cells&nbsp;through NKG2D/RAE-1 pathway, meanwhile, RAE-1 has a negative feedback effect on NK/T cells and consequently in-hibits the killing activity of immune cells against the tumor cells .NKG2D/RAE1 pathway can be used as immunotherapy target for hypophysoma .%目的：观察NKG2D／RAE-1通路在介导自然杀伤（NK）细胞、CD8＋T细胞（两种细胞简称NK／T细胞）杀伤垂体瘤细胞中的作用，从细胞学水平探讨垂体瘤的免疫逃逸机制。

方法以小鼠垂体瘤细胞系AtT20为研究对象，采用免疫荧光标记法检测NKG2 D配体RAE-1在垂体瘤细胞的表达水平及定位分布情况。

采用NK／T细胞分选试剂盒分离NK／T细胞，将NK／T细胞放入Transwell小室的上室，靶细胞AtT20置入Transwell小室的下室，随机分为对照组、实验组。

对照组在常规条件下共培养0、4、8、24 h，实验组在培养液中加入阻断RAE-1的抗体（ Anti-RAE1抗体）共培养0、4、8、24 h；采用MTT法检测两组各时间点细胞增殖抑制率。

结果 RAE-1在AtT20细胞膜明显表达，在细胞质内亦有弱表达。

在AtT20细胞中RAE-1蛋白阳性表达率为100％，其中强表达率为30％、中表达率为60％、弱表达率为10％，无阴性表达。

对照
组培养4、8、24 h时细胞增殖抑制率明显高于0 h时（P均＜0．05）；实验组培养0、4、8、24 h时增殖抑制率逐渐升高（P均＜0．05），培养8、24 h时增殖抑制率均明显高于同时间点对照组（P均＜0．05）。

结论 NK／T细胞通过NKG2D／RAE-1通路杀灭垂体瘤细胞，RAE-1可负反馈作用于NK／T细胞，从而抑制免疫细胞对肿瘤细胞的杀伤活性。

NKG2D／RAE-1通路可能作为垂体瘤的免疫治疗靶点。

【总页数】4页(P4-7)
【作者】孙其凯;马立新;李珍珠;陈正;李泽福
【作者单位】滨州医学院附属医院，山东滨州256600;滨州医学院附属医院，山东滨州256600;滨州医学院附属医院，山东滨州256600;滨州医学院附属医院，山东滨州256600;滨州医学院附属医院，山东滨州256600
【正文语种】中文
【中图分类】R736.4
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