益生菌的筛选及安全性研究进展.

cacy of MS -222and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Puntius filamento －sus (Valenciennes [J].Aquaculture research, 2010,41(2:309-314

[17]刘长琳, 何力, 陈四清, 等. 鱼类麻醉研究综述[J].渔业现代化,

2007,34(5:21-25

[18]Kiessling A,Johansson D,Zahl I H, et al. Pharmacokinetics,plasma

cortisol and effectiveness of benzocaine,MS -222and isoeugenol measured in individual dorsal aorta -cannulated Atlantic salmon

(Salmo salar following bath administration[J].Aquaculture,2009,

286(3/4:301-308

[19]Tang S,Thorarensen H,Brauner C J,et al. Modeling the accumulation

of CO 2during high density,re-circulating transport of adult Atlantic salmon,Salmo salar,from observations aboard a sea -going commer －cial live-haul

vessel[J].Aquaculture,2009,296(1/2:288-292[20]Velisek J,Svobodova Z,Piackova V. Effects of clove oil anaesthesia

on Rainbow Trout (Oncorhynchus mykiss[J].Acta Veterinaria Brno, 2005(74:139-146

[21]Park M O,Im S Y,Seol D W,et al. Efficacy and physiological re －

sponses of rock bream, Oplegnathus fasciatus to anesthetization with clove

oil[J].Aquaculture, 2009,287(3/4:427-430

[22]Iversen M,Eliassen R A. The Effect of AQUI -S -(R Sedation on

Primary,Secondary,and Tertiary Stress Responses during Salmon Smolt,Salmo salar L. Transport and Transfer to Sea[J].Journal of the World Aquaculture Society,2009,

40(2:216-225

[23]赵艳丽, 杨先乐, 黄艳萍, 等. 丁香酚对大黄鱼麻醉效果的研究[J].

水产科技报,2002,29(4:163-165

[24]Coyle S D,Durborow R M,Tidwell J H. Anesthetics in Aquaculture

[R].Southern Regional Aquaculture Center (SRACPublication, 2004, No. 3900

收稿日期：2012-03-19

益生菌的筛选及安全性研究进展

董秋月，陈檬燕，辛杨，秦雪艳，程卓，石陆娥*

（杭州师范大学生命与环境科学学院，浙江杭州310036）

摘

要：评价益生菌安全性的方法很多，如研究益生菌的特性、益生菌的药物动力学及益生菌与宿主之间的相互作用

等。随着研究的不断深入，益生菌独特的营养与保健作用越来越受到人们广泛关注。就益生菌的筛选鉴定，生物安全性以及功能的最新研究进展作一综述。关键词：益生菌；安全性；筛选；研究

Safety Research and Selection in Probiotics

DONG Qiu-yue ，CHEN Meng-yan ，XIN Yang ，QIN Xue-yan ，CHENG Zhuo ，SHI Lu-e *

（College of Life and Environmental Sciences ，Hangzhou Normal University ，Hangzhou 310036，Zhejiang ，

China ）

Abstract ：Evaluation of security of Probiotics have many methods ，such as studying the characteristics of Probiotics ，the pharmacokinetics of Probiotics ，the interaction between the Probiotics and the host and so on. With the deepening of the study ，Probiotics ′unique nutrition and he alth-care effect have got more and more peoples ′attention. The paper will focus on the latest research progress of Probiotics screening and identification ，bio-security and function.

Key words ：Probiotics ；safety ；screening ；research

食品研究与开发

F ood Research And Development

作者简介：董秋月（1992—），女（汉），本科，研究方向：生物技术。*通信作者：石陆娥（1979—），女（汉），副教授，博士，研究方向：生物技术。

随着生活水平的提高，大家开始更多地关注健康，人们对食品的要求也不再仅限于满足最基本的生理需求。于是，

像益生菌、益生元这类有益于身体健康的食品添加剂逐渐吸引了人们的眼球。虽然目前益生菌的多种益生性能已经被大家所认可，但是益生菌制品却没有得到充分的开发。这主要是因为益生菌的保藏不易，更重要的是益生菌的种类繁多。筛选出动物体内的不同益生菌，研究益生菌的生理生化特性，进

行安全性鉴定是近年来微生物研究中重要的重要组