外周血MAIT细胞数量在肝细胞癌患者诊断及预后中的应用研究
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中西医结合肝病杂志2019年第29卷第5期
・415・
doi: 10. 3969/j. issn. 1005 -0264.2019.05.010
外周血MAIT细胞数量在肝细胞癌患者诊断及预后中的 应用研究
胡晟
重庆市第四人民医院肝病科(重庆，400000)
摘要目的:对比正常人与肝细胞癌患者外周血中黏膜相关恒定T细胞(MAIT)的数量，明确外周血MAIT细胞数量在肝细 胞癌临床诊断与预后判断中的应用价值。方法：以32例肝细胞癌患者作为实验组，以32例健康体检者作为对照组,采集两组患者 外周血并通过流式细胞术对比两组惠者外周血中MAIT细胞百分比及绝对数量。统计分析肝细胞癌患者外周血MAIT细胞数量与 年龄、性别、肿瘤大小、结节数量、远处转移、甲胎蛋白.Child-Pugh分期、TNM分期的相关性。通过R0C曲线分析外周血MAIT细胞 数量在肝细胞癌中的诊断效能比较高MAIT水平患者和低MAIT水平患者的生存时间和预后。结果:肝细胞癌患者外周血中MAIT 细胞占T细胞百分比显著低于正常健康体检者,且其外周血中MAIT细胞数量绝对值也明显低于正常人。此外，肝细胞癌患者外周 血MAIT细胞数量与年龄、性别、肿瘤大小、结节数量、肝功能分期无相关性，在有远处转移、AFP >400 jig/L、TNM分期为III-IV期的 肝细胞癌患者中外周血MAIT细胞数量较高。ROC曲线提示外周血MAIT细胞数量的AUG为0.885,可作为肝细胞癌诊断标志物。 生存曲线分析提示外周血高MAIT水平(>25.78细胞/讪)的肝细胞癌患者生存时间明显高于低MAIT水平的患者。结论:肝细胞 癌患者外周血MAIT水平，可以作为肝细胞癌的诊断及预后判断指标,具有较好的临床应用价值。
关键词肝细胞癌;黏膜相关恒定T细胞;诊断;预后
Application research of mucosa-associated invariant T cell of peripheral blood in the diagnosis and prognosis of hepatocellular carcinoma
HU Sheng. Chongqing Fourth People's Hospital ( Chongqing, 400000) ChБайду номын сангаасna
Abstract Objective:To compare the number of mucosa-associated invariant T ( MAIT) cells in peripheral blood between healthy donors and patients with hepatocellular carcinoma, and to illustrate the clinical use of MAIT cells in peripheral blood in the diagnosis and prognosis of hepatocellular carcinoma. Methods: A total of 32 patients with hepatocellular carcinoma were recruited as the experimental group, and 32 healthy donors were recruited as the control group. Peripheral blood samples of these two groups were collected and their MAIT cells ratio and absolute number were analyzed using flow cytometry. The correlation among the MAIT cells number in peripheral blood and age, sexual, tumor size, numbers of nodules, distant metastasis, AFP, Child-Pugh stage and TNM stage were analyzed. The diagnostic efficiency of MAIT cells number in peripheral blood in hepatocellular carcinoma was calculated using ROC curve. The survival time and prognosis between the hepatocellular carcinoma patients with high MAIT cells and those with low MAIT cells were compared by the survivorship curve. Results: The ratio of MAIT cells in T cells in peripheral blood of hepatocellular carcinoma patients was significantly lower than that in healthy donors. The absolute number of MAIT cells in peripheral blood of hepatocellular carcinoma patients was also lower than that in healthy donors. Besides, there were no relationships among the MAIT cells number and the age, sexual, tumor size, numbers of nodules, Child-Pugh stage in hepato cellular carcinoma patients. But in the hepatocellular carcinoma patients with distant metastasis, AFP higher than 40Qhg/L and III-IV stages
・415・
doi: 10. 3969/j. issn. 1005 -0264.2019.05.010
外周血MAIT细胞数量在肝细胞癌患者诊断及预后中的 应用研究
胡晟
重庆市第四人民医院肝病科(重庆，400000)
摘要目的:对比正常人与肝细胞癌患者外周血中黏膜相关恒定T细胞(MAIT)的数量，明确外周血MAIT细胞数量在肝细 胞癌临床诊断与预后判断中的应用价值。方法：以32例肝细胞癌患者作为实验组，以32例健康体检者作为对照组,采集两组患者 外周血并通过流式细胞术对比两组惠者外周血中MAIT细胞百分比及绝对数量。统计分析肝细胞癌患者外周血MAIT细胞数量与 年龄、性别、肿瘤大小、结节数量、远处转移、甲胎蛋白.Child-Pugh分期、TNM分期的相关性。通过R0C曲线分析外周血MAIT细胞 数量在肝细胞癌中的诊断效能比较高MAIT水平患者和低MAIT水平患者的生存时间和预后。结果:肝细胞癌患者外周血中MAIT 细胞占T细胞百分比显著低于正常健康体检者,且其外周血中MAIT细胞数量绝对值也明显低于正常人。此外，肝细胞癌患者外周 血MAIT细胞数量与年龄、性别、肿瘤大小、结节数量、肝功能分期无相关性，在有远处转移、AFP >400 jig/L、TNM分期为III-IV期的 肝细胞癌患者中外周血MAIT细胞数量较高。ROC曲线提示外周血MAIT细胞数量的AUG为0.885,可作为肝细胞癌诊断标志物。 生存曲线分析提示外周血高MAIT水平(>25.78细胞/讪)的肝细胞癌患者生存时间明显高于低MAIT水平的患者。结论:肝细胞 癌患者外周血MAIT水平，可以作为肝细胞癌的诊断及预后判断指标,具有较好的临床应用价值。
关键词肝细胞癌;黏膜相关恒定T细胞;诊断;预后
Application research of mucosa-associated invariant T cell of peripheral blood in the diagnosis and prognosis of hepatocellular carcinoma
HU Sheng. Chongqing Fourth People's Hospital ( Chongqing, 400000) ChБайду номын сангаасna
Abstract Objective:To compare the number of mucosa-associated invariant T ( MAIT) cells in peripheral blood between healthy donors and patients with hepatocellular carcinoma, and to illustrate the clinical use of MAIT cells in peripheral blood in the diagnosis and prognosis of hepatocellular carcinoma. Methods: A total of 32 patients with hepatocellular carcinoma were recruited as the experimental group, and 32 healthy donors were recruited as the control group. Peripheral blood samples of these two groups were collected and their MAIT cells ratio and absolute number were analyzed using flow cytometry. The correlation among the MAIT cells number in peripheral blood and age, sexual, tumor size, numbers of nodules, distant metastasis, AFP, Child-Pugh stage and TNM stage were analyzed. The diagnostic efficiency of MAIT cells number in peripheral blood in hepatocellular carcinoma was calculated using ROC curve. The survival time and prognosis between the hepatocellular carcinoma patients with high MAIT cells and those with low MAIT cells were compared by the survivorship curve. Results: The ratio of MAIT cells in T cells in peripheral blood of hepatocellular carcinoma patients was significantly lower than that in healthy donors. The absolute number of MAIT cells in peripheral blood of hepatocellular carcinoma patients was also lower than that in healthy donors. Besides, there were no relationships among the MAIT cells number and the age, sexual, tumor size, numbers of nodules, Child-Pugh stage in hepato cellular carcinoma patients. But in the hepatocellular carcinoma patients with distant metastasis, AFP higher than 40Qhg/L and III-IV stages