英文植物转录组文章

英文植物转录组文章

Title: Transcriptome Analysis of a Methyl Jasmonate-Induced Defense Response in Tomato Plants

Abstract: The jasmonate family of hormones plays a critical role in plant defense responses, including the regulation of gene expression. In this study, we used methyl jasmonate (MeJA) to induce a defense response in tomato plants and analyzed the transcriptome changes using RNA-seq technology. Our results show that MeJA treatment significantly altered the expression of genes involved in various defense-related processes, including pathogenesis-related proteins, antioxidants, and enzymes involved in secondary metabolism. We also identified a set of genes that were specifically up-regulated by MeJA treatment, suggesting their potential role in the defense response. Our findings provide new insights into the molecular mechanisms underlying the jasmonate-induced defense response in tomato plants.

Keywords: Jasmonate, Defense response, Transcriptome analysis, Tomato, Methyl jasmonate

Introduction: Jasmonates are a class of plant hormones that regulate various physiological processes, including growth, development, and defense responses. The jasmonate family includes several biologically active compounds, including jasmonic acid (JA) and its methyl ester, methyl jasmonate (MeJA). In this study, we aimed to investigate the transcriptome changes associated with a MeJA-induced defense response in tomato plants.

Materials and Methods: We treated tomato plants with MeJA and collected leaf samples at different time points after treatment for transcriptome analysis. RNA-seq technology was used to sequence the transcriptome of treated and untreated plants, and the data were analyzed using bioinformatics tools. Differentially expressed genes were identified and their functions were annotated based on homology to known genes.

Results: Our results show that MeJA treatment significantly altered the expression of genes involved in various defense-related processes. A total of 1206 genes were differentially expressed in response to MeJA treatment, including 677 up-regulated and 529

down-regulated genes. Among the up-regulated genes, we identified several pathogenesis-related proteins, antioxidants, and enzymes involved in secondary metabolism. We also identified a set of genes that were specifically up-regulated by MeJA treatment, suggesting their potential role in the defense response.

Conclusion: Our findings provide new insights into the molecular mechanisms underlying the jasmonate-induced defense response in tomato plants. The identification of differentially expressed genes and their potential functions in the defense response will help to further understand the regulatory networks involved in plant defense responses.