蛋白Marker说明书

SM0671
PageRuler Prestained Protein Ladder
A prestained SDS-PAGE MW marker with contrasting colored reference bands at
10 and 70kDa.
The Thermo Scientific PageRuler Prestained Prote
is a mixture of ten (10) blue-, orange- and green-s
recombinant proteins (10 to 170kDa) for use as siz
standards in protein electrophoresis (SDS-PAGE)
Western blotting.
This prestained protein MW marker is designed fo
monitoring the progress of SDS-polyacrylamide ge
electrophoresis, for assessing transfer efficiency o
nylon and nitrocellulose membranes, and for estim
approximate size of separated proteins that have b visible with gel stains or Western blot detection reagents. The ladder contains one orange refer at 70kDa and one green band at 10kDa.
Highlights:
•Size range– 10 proteins spanning 10 to 170kDa
•Ready-to-use– supplied in a loading buffer for direct loading on gels; no need to boil •Sharp bands– color-coded bands of similar intensity for easy visualization
•Quality tested – each lot evaluated by SDS-PAGE and Western blotting
•Two reference bands– orange at 70kDa and green at 10kDa
•Membrane-compatible– colored bands transfer to membranes for Western blotting
Includes:
•Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 1 1mM NaN3 and 33% glycerol.
Applications:
•Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
•Monitoring protein transfer onto membranes after Western blotting
•Sizing of proteins on SDS-polyacrylamide gels and Western blots
Product Details:
SDS-PAGE band profile of the Thermo Scientific
PageRuler Prestained Protein Ladder. Images are
from a 4-20% Tris-glycine gel (SDS-PAGE) and
subsequent transfer to membrane.
Sm1841
Spectra Multicolor Broad Range Protein Ladder
A prestained, 4-color, SDS-PAGE MW marker especially for proteins between 10 and 260kDa.
The Thermo Scientific Spectra Multicolor Broad Range
Protein Ladder is a 4-color protein standard containing 10
prestained recombinant prokaryotic proteins (10 to 260kDa)
for use as size standards in gel electrophoresis and Western
blotting.
This prestained protein MW marker is designed for
monitoring the progress of SDS-polyacrylamide gel
electrophoresis, for assessing transfer efficiency onto PVDF,
nylon and nitrocellulose membranes, and for estimating the
approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Four different chromophores (blue, orange, green, pink) are bound to the different component proteins, producing a brightly colored ladder with an easy-to-remember pattern.
Highlights:
•Size range– 10 proteins spanning 10 to 260kDa
•Multicolor– four different colors for unambiguous band-size assignment
•Ready-to-use– supplied in a loading buffer for direct loading on gels; no need to boil
•Sharp bands– color-coded bands of similar intensity for easy visualization
•Quality tested – each lot evaluated by SDS-PAGE and Western blotting
•Membrane-compatible– colored bands transfer to membranes for Western blotting Includes:
•Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Applications:
•Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
•Monitoring protein transfer onto membranes after Western blotting
•Sizing of proteins on SDS-polyacrylamide gels and Western blots
Product Details:
SDS-PAGE band profile of the Thermo Scientific
Spectra Multicolor Broad Range Protein Ladder.
Images are from a 4-20% Tris-glycine gel
(SDS-PAGE) and subsequent transfer to membrane.
S m1811
PageRuler Plus Prestained Protein Ladder
A prestained SDS-PAGE MW marker with contrasting colored reference bands at 10, 25 and 70kDa.
The Thermo Scientific PageRuler Plus Prestained Protein
Ladder is a mixture of nine (9) blue-, orange- and
green-stained recombinant proteins (10 to 250kDa) for use
as size standards in protein electrophoresis (SDS-PAGE)
and Western blotting.
This prestained protein MW marker is designed for
monitoring the progress of SDS-polyacrylamide gel
electrophoresis, for assessing transfer efficiency onto PVDF,
nylon and nitrocellulose membranes, and for estimating the
approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. A blue chromophore is bound to all proteins, except proteins of two reference bands of 70kDa and 25kDa that are colored with an orange dye and one green reference band of 10kDa. PageRuler Plus Prestained Protein Ladder is ready to use: no heating, further dilution or addition of a reducing agent is required before loading onto a gel.
Highlights:
•Size range– nine proteins spanning 10 to 250kDa
•Ready-to-use– supplied in a loading buffer for direct loading on gels; no need to boil
•Sharp bands– color-coded bands of similar intesity for easy visualization
•Quality tested – each lot evaluated by SDS-PAGE and Western blotting
•Bright reference bands– orange at 70 and 25kDa, and green at 10kDa
•Membrane-compatible– colored bands transfer to membranes for Western blotting Includes:
•Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Applications:
•Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
•Monitoring protein transfer onto membranes after Western blotting
•Sizing of proteins on SDS-polyacrylamide gels and Western blots
Product Details:
SDS-PAGE band profile of the Thermo Scientific PageRuler Plus Prestained Protein Ladder. Images are from a 4-20% Tris-glycine gel
(SDS-PAGE) and subsequent transfer to membrane.。