The GraF instrument for imaging spectroscopy with the adaptive optics

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光谱仪器原理

光谱仪器原理

Spectrographys are optical instruments that form images S2(λ) of the entrance slit S1;the images are laterally separated for different wavelengths λof the incident radiation.Ω=F/f12受棱镜的有效面积F=h.a的限制,它代表光的限制孔径.的方式成像到入射狭缝上是有利的,虽然会聚透镜可以缩小光源在入射狭经上所成的像,使更多的来自扩展光源的辐射功率通过入射狭缝:但是发散度增大了.在接收角外的辐射不能被探测到,反而增大了由透镜支架和分光计任何色散型仪器的光谱分辨本领的定义为和λ2间的最小间隔.-λ2)在二个最大间显示出明显的凹陷,则可以认为强度分布是由具有强度轮廓为I1(λ-λ1)和I21(λ-λ2)的二条)依赖于比率I1/I2和二个分量的轮廓,因此最小对于不同的轮廓将是不相同.2的第一最小重合,则认为两条谱线如果强度相等的两条线的两个最大间的凹陷降到I的(8/π2)≈0.8,(a)Diffraction in a spectrometer by the limiting aperture with diameter af1f2:angular dispersion[rad/nm]成像在平面B上)间的距离△x2为=(dx/dλ)△λ:linear dispersion of the instrument,[mm/nm]为了分辨λ和λ+△λ的二条线,上式中的间距△x2至少应为二个狭缝象的宽度(λ)+δx2(λ+△λ),由于宽度x2由下式与入射狭缝宽度相联系:δx2=(f2/f1) δx1所以减小δx1便能增大分辨本领λ/△λ,可惜存在着由衍射造成的理论极限.由于分辨极限十分重要.我们将对这点作更详细的讨论.(b)Limitation of spectral resolution by diffraction=±λ/b间(见图);仅当2 δΦ小于分光计的接收角a/f1时,它才能完全通过限制孔径a.这给出入射狭缝有效宽度bmin的下限为在一切实际情形中,入射光都是发散的.这就要求发散角和衍射角之和必须小于,而最小狭缝相应地更大。

肌骨超声联合CT_能谱成像在不同临床时期痛风患者诊断中的敏感度和准确性分析

肌骨超声联合CT_能谱成像在不同临床时期痛风患者诊断中的敏感度和准确性分析

肌骨超声联合CT能谱成像在不同临床时期痛风患者诊断中的敏感度和准确性分析*黄海霞① 温小芳① 彭诚初① 黄旭胜① 【摘要】 目的:研究肌骨超声联合CT能谱成像在痛风患者不同临床时期的应用价值。

方法:回顾性分析2021年1月—2023年6月在广州医科大学附属惠州医院超声科进行肌骨超声检查、CT能谱检查的66例疑似痛风患者的临床资料。

以关节穿刺和镜检尿酸单钠晶体为诊断“金标准”,对比单一肌骨超声、CT能谱成像诊断和二者联合诊断对痛风的诊断效能(敏感度、准确性)及对痛风不同临床时期的诊断符合率(急性期、间歇发作期、慢性关节炎期)。

结果:肌骨超声联合CT能谱成像的诊断敏感度(100%)、准确性(96.97%)均高于单一肌骨超声(85.96%、77.27%)、CT能谱成像(89.47%、81.82%)(P<0.05)。

肌骨超声联合CT能谱成像对急性期、间歇发作期、慢性关节炎期的诊断符合率均高于单一肌骨超声、CT能谱成像(P<0.05)。

结论:在痛风患者中采用肌骨超声联合CT能谱成像诊断,能提高诊断敏感度、准确性,并明确患者的临床时期,在临床治疗中具有重要指导价值。

【关键词】 痛风 临床时期 肌骨超声 CT能谱成像 敏感度 准确性 Sensitivity and Accuracy Analysis of Muscle Bone Ultrasound Combined with CT Energy SpectrumImaging in Different Clinical Stages of Gout Patients/HUANG Haixia, WEN Xiaofang, PENG Chengchu,HUANG Xusheng. //Medical Innovation of China, 2024, 21(10): 097-100 [Abstract] Objective: To study the application value of muscle bone ultrasound combined with CT energyspectrum imaging in different clinical stages of gout patients. Method: A retrospective analysis was conducted onthe clinical data of 66 patients with suspected gout who underwent muscle bone ultrasound and CT energy spectrumexamination in Huizhou Hospital Affiliated to Guangzhou Medical University from January 2021 to June 2023. Jointpuncture and microscopic examination of monosodium urate crystals were used as the diagnostic "gold standard",The diagnostic efficacy (sensitivity, accuracy) of and single muscle bone ultrasound, CT energy spectrum imagingdiagnosis and the combination of the two for gout were compared, as well as the diagnostic coincidence rate for goutat different clinical stages (acute stage, intermittent attack stage, chronic arthritis stage), while the muscle boneultrasound and CT energy spectrum imaging characteristics of gout patients at different clinical stages were analyzed.Result: The diagnostic sensitivity (100%) and accuracy (96.97%) of muscle bone ultrasound combined with CTenergy spectrum imaging were higher than those of single muscle bone ultrasound (85.96%, 77.27%) and CT energyspectrum imaging (89.47%, 81.82%) (P<0.05). The diagnostic accuracy of muscle bone ultrasound combined withCT energy spectrum imaging in acute stage, intermittent attack stage, and chronic arthritis stages was higher thanthose of single muscle bone ultrasound and CT energy spectrum imaging (P<0.05). Conclusion: The use of musclebone ultrasound combined with CT energy spectrum imaging in gout patients can improve diagnostic sensitivityand accuracy, and accurately determine the clinical period of the patient. It has important guiding value in clinicaltreatment. [Key words] Gout Clinical stage Muscle bone ultrasound CT energy spectrum imaging Sensitivity Accuracy First-author's address: Department of Ultrasound, Huizhou Hospital Affiliated to Guangzhou MedicalUniversity, Huizhou 516002, China doi:10.3969/j.issn.1674-4985.2024.10.022*基金项目:惠州市科技计划项目(210422114572931)①广州医科大学附属惠州医院(惠州市第三人民医院)超声科 广东 惠州 516002通信作者:黄海霞- 97 - 痛风是一种由嘌呤代谢失衡和/或尿酸排泄障碍而导致长期血尿酸增高、尿酸盐晶体广泛沉积引起的反复发作性炎症疾病[1-2]。

傅里叶变换红外光谱仪英文

傅里叶变换红外光谱仪英文

傅里叶变换红外光谱仪英文Fourier Transform Infrared SpectrometerIntroduction:The Fourier Transform Infrared (FTIR) spectrometer is an essential tool in the field of spectroscopy. It utilizes the mathematical technique known as Fourier transform to analyze infrared light, enabling scientists to study the molecular composition and structure of various substances. In this article, we will explore the principles behind the Fourier Transform Infrared Spectrometer and its applications in scientific research.Principles of Fourier Transform Infrared Spectroscopy:Fourier Transform Infrared Spectroscopy is based on the interaction between infrared light and matter. When a substance is exposed to infrared radiation, the energy absorbed by the molecules causes them to vibrate. These vibrations are specific to each molecule and are dependent on the molecular bonds present within the substance.The spectrometer operates by passing an infrared beam through the sample and measuring the amount of light absorbed at different wavelengths. This absorption spectrum is then transformed using Fourier analysis, producing a highly detailed and accurate representation of the substance's molecular structure.Advantages of Fourier Transform Infrared Spectroscopy:1. High Speed and Sensitivity: Fourier Transform Infrared Spectroscopy offers rapid analysis times due to its ability to gather a full range ofwavelengths simultaneously. This allows for efficient data collection, making it ideal for high-throughput applications. Additionally, the technique is highly sensitive, capable of detecting even small quantities of sample material.2. Broad Analytical Range: FTIR spectroscopy covers a wide range of wavelengths, from near-infrared (NIR) to mid-infrared (MIR). This versatility enables the analysis of various substances, including organic and inorganic compounds, polymers, pharmaceuticals, and biological samples.3. Non-destructive Analysis: One of the key advantages of FTIR spectroscopy is that it is a non-destructive technique. Samples do not require any special preparation and can be analyzed directly, allowing for subsequent analysis or retesting if required.Applications of Fourier Transform Infrared Spectrometers:1. Pharmaceutical Analysis: FTIR spectroscopy plays a vital role in drug discovery and development. It is used to identify and characterize the molecular composition of active pharmaceutical ingredients (APIs), excipients, and impurities. By comparing spectra, scientists can ensure the quality and purity of pharmaceutical products.2. Environmental Analysis: Fourier Transform Infrared Spectrometers are employed in environmental monitoring to analyze air, water, and soil samples. It aids in detecting pollutants, identifying unknown substances, and assessing the impact of human activities on the environment.3. Forensic Science: FTIR spectroscopy has proven to be a valuable tool in forensic science. It assists in the analysis of various evidence, such asfibers, paints, and drugs. FTIR spectra can provide crucial information in criminal investigations, helping to identify unknown substances and link them to potential sources.4. Food and Beverage Industry: The FTIR spectrometer allows for the analysis of food quality, safety, and authenticity. It can identify contaminants, detect adulteration, and verify product labeling claims. Both raw materials and finished products can be analyzed using this technique, ensuring compliance with industry regulations.Conclusion:The Fourier Transform Infrared Spectrometer has revolutionized the field of spectroscopy by providing accurate and detailed information about a substance's molecular structure. Its speed, sensitivity, and versatility make it a crucial analytical tool in various scientific disciplines. With ongoing advancements in technology, FTIR spectroscopy continues to contribute to new discoveries and advancements in research.。

offner成像光谱仪的设计方法

offner成像光谱仪的设计方法

offner成像光谱仪的设计方法英文回答:Designing an Offner imaging spectrometer involves several key steps and considerations. The Offner configuration is a popular choice for its compactness and ability to provide high spectral resolution. Here is a step-by-step guide to designing an Offner imaging spectrometer:1. Determine the spectral range and resolution requirements: The first step is to define the desired spectral range and resolution for the spectrometer. This will depend on the specific application and the types of samples or phenomena that need to be analyzed.2. Select the correct optics: The Offner configuration consists of two concave mirrors and a convex grating. The choice of optics is crucial to achieve the desired performance. The mirrors should have a high reflectivityand low scattering, while the grating should have a high diffraction efficiency and low stray light.3. Calculate the design parameters: The design parameters of the Offner spectrometer include the focal lengths of the mirrors, the radius of curvature of the grating, and the distance between the mirrors. These parameters need to be carefully calculated to ensure proper imaging and dispersion.4. Consider aberrations: Offner spectrometers are prone to various aberrations, such as astigmatism and coma. These aberrations can degrade the spectral and spatial resolution. It is important to analyze and minimize these aberrations through careful design and optimization.5. Optimize the system: Once the initial design is complete, it is necessary to optimize the system for better performance. This can involve adjusting the mirror curvatures, grating position, or other parameters toachieve the desired spectral resolution and image quality.6. Test and calibrate: After the design and optimization, the Offner spectrometer needs to be tested and calibrated. This involves measuring the spectral and spatial resolution, as well as characterizing any remaining aberrations or distortions. Calibration methods, such as using known spectral sources or calibration standards, can help ensure accurate measurements.7. Consider practical constraints: Finally, it is important to consider practical constraints in the design, such as size, weight, and cost. Offner spectrometers can be quite compact, but trade-offs may need to be made to meet specific requirements.中文回答:设计Offner成像光谱仪涉及到几个关键步骤和考虑因素。

可见光光谱 英文

可见光光谱 英文

可见光光谱英文The visible light spectrum, encompassing wavelengths ranging from approximately 400 nanometers (nm) to 700 nm,is a narrow slice of the electromagnetic radiation that our eyes are capable of perceiving. This band of wavelengths, although relatively small compared to the vast expanse of the electromagnetic spectrum, plays a pivotal role in our daily lives, shaping our perception of the world around us. At the shorter wavelength end of the visible spectrum, we encounter violet light. Violet waves, with their frequencies exceeding 668 THz, are the highest in energy among all visible colors. As we move towards the red end of the spectrum, wavelengths increase, resulting in lower frequencies and consequently, lower energy levels. Red light, with wavelengths exceeding 700 nm, has the lowest energy among all visible colors.The visible spectrum is not just a random assortment of colors; it is a carefully crafted array of hues that enables us to perceive a wide range of colors. The human eye is equipped with photoreceptors called cones, which are sensitive to specific wavelengths within the visiblespectrum. These cones are primarily sensitive to blue, green, and red light, allowing us to perceive the full range of colors visible to the naked eye.The importance of the visible light spectrum extends beyond our ability to see colors. It plays a crucial role in photosynthesis, the process by which plants convert light energy into chemical energy. Chlorophyll, the green pigment found in plants, is highly absorbent of blue and red light wavelengths, which are essential for photosynthesis. Without the visible light spectrum, photosynthesis would not be possible,严重影响着整个生态系统的运转。

傅里叶红外光谱仪英文

傅里叶红外光谱仪英文

傅里叶红外光谱仪英文傅里叶红外光谱仪英文IntroductionFourier transform infrared spectroscopy (FTIR) is a powerful analytical technique used to identify the chemical composition of a sample based on its molecular vibrations. FTIR spectrometers have various ranges, including mid-infrared (MIR), near-infrared (NIR), and far-infrared (FIR). In this article, we will focus on the Fourier transform infrared spectrometer in the mid-infrared range, also known as FTIR-MIR.InstrumentationFTIR-MIR spectrometers consist of a light source, a sample compartment, a detector, and an interferometer. The interferometer is the heart of the FTIR instrument, as it converts the sample's spectral signal from a time domain to a frequency domain. The signal is then detected by a detector and processed through a computer. The main components of the MIR-FTIR spectrometer include:1. Light source: Typically, a high-intensity, high-resolution infrared source is used, such as a Globar or a mercury-cadmium-telluride (MCT) detector.2. Sample compartment: This is where the sample is placed for analysis. Samples can be in the form of liquids, solids, gases, or films.3. Interferometer: This is the key component of the FTIR-MIR spectrometer.There are several types of interferometers, including Michelson and Fourier transform.4. Detector: The detector is used to detect the spectral signal from the interferometer and generate an electrical signal that is then processed and displayed on the computer.ApplicationsFTIR-MIR spectrometers are widely used in various industries, including pharmaceuticals, chemistry, polymers, food, and environmental analysis. This technique is used to identify and characterize chemical compounds, determine the purity of samples, identify unknown compounds, and monitor chemical reactions. FTIR-MIR can also be used to detect and quantify gases and pollutants in the atmosphere.AdvantagesFTIR-MIR spectrometers have several advantages over other analytical techniques, including:1. Non-destructive analysis: FTIR-MIR analysis does not destroy the sample, allowing for further analysis if necessary.2. Fast analysis: The analysis time for FTIR-MIR is usually less than a minute, making it a quick and efficient technique for sample analysis.3. High sensitivity: FTIR-MIR spectrometers can detect trace amounts of compounds, making it possible to identify small impurities in a sample.4. Versatility: FTIR-MIR can be used to analyze a wide range of sample types, including liquids, solids, gases, and films.ConclusionFourier transform infrared spectrometry is a powerful analytical technique that can provide valuable information in various industries. With its non-destructive analysis, fast analysis time, high sensitivity, and versatility, FTIR-MIR spectrometers are essential tools for chemical analysis and pollution monitoring.。

荧光成像基础知识

荧光成像基础知识

12
SCHOOL of FLUORESCENCE
MPSF educator packet
This packet contains illustrations and figures from the Molecular Probes® School of Fluorescence website. They illustrate concepts from the basic physical properties that underlie fluorescence through experiment planning and troubleshooting. The images and graphics on this page are copyrighted, but they are freely available for your use as long as the attribution “Molecular Probes®” remains intact.
10
SCHOOL of FLUORESCENCE
Part One: Fundamentals of Fluorescence Microscopy
Figure 2.2. The light path through lenses and sample in basic brightfield microscopy (A). Antique 19th century drum-style compound microscope (B).
Figure 1.3. The inverse relationship between energy and wavelength in the visible spectrum.

纹理物体缺陷的视觉检测算法研究--优秀毕业论文

纹理物体缺陷的视觉检测算法研究--优秀毕业论文

摘 要
在竞争激烈的工业自动化生产过程中,机器视觉对产品质量的把关起着举足 轻重的作用,机器视觉在缺陷检测技术方面的应用也逐渐普遍起来。与常规的检 测技术相比,自动化的视觉检测系统更加经济、快捷、高效与 安全。纹理物体在 工业生产中广泛存在,像用于半导体装配和封装底板和发光二极管,现代 化电子 系统中的印制电路板,以及纺织行业中的布匹和织物等都可认为是含有纹理特征 的物体。本论文主要致力于纹理物体的缺陷检测技术研究,为纹理物体的自动化 检测提供高效而可靠的检测算法。 纹理是描述图像内容的重要特征,纹理分析也已经被成功的应用与纹理分割 和纹理分类当中。本研究提出了一种基于纹理分析技术和参考比较方式的缺陷检 测算法。这种算法能容忍物体变形引起的图像配准误差,对纹理的影响也具有鲁 棒性。本算法旨在为检测出的缺陷区域提供丰富而重要的物理意义,如缺陷区域 的大小、形状、亮度对比度及空间分布等。同时,在参考图像可行的情况下,本 算法可用于同质纹理物体和非同质纹理物体的检测,对非纹理物体 的检测也可取 得不错的效果。 在整个检测过程中,我们采用了可调控金字塔的纹理分析和重构技术。与传 统的小波纹理分析技术不同,我们在小波域中加入处理物体变形和纹理影响的容 忍度控制算法,来实现容忍物体变形和对纹理影响鲁棒的目的。最后可调控金字 塔的重构保证了缺陷区域物理意义恢复的准确性。实验阶段,我们检测了一系列 具有实际应用价值的图像。实验结果表明 本文提出的纹理物体缺陷检测算法具有 高效性和易于实现性。 关键字: 缺陷检测;纹理;物体变形;可调控金字塔;重构
Keywords: defect detection, texture, object distortion, steerable pyramid, reconstruction
II

高光谱英文缩写

高光谱英文缩写

高光谱英文缩写Hyperspectral imaging, often referred to as HSI, is a powerful and versatile technology that has revolutionized the way we perceive and analyze the world around us. This advanced imaging technique goes beyond the capabilities of traditional digital cameras by capturing a vast array of spectral information from the electromagnetic spectrum, providing a wealth of data that can be used in a wide range of applications.At its core, hyperspectral imaging involves the acquisition of high-dimensional data cubes, where each pixel in the image contains a detailed spectral signature. This signature represents the unique reflectance or emission characteristics of the target material, allowing for the identification and classification of a wide variety of substances and materials. Unlike conventional RGB (red, green, blue) imaging, which captures only three color channels, hyperspectral sensors can record hundreds or even thousands of narrow spectral bands, creating a rich and detailed spectral profile.The power of hyperspectral imaging lies in its ability to revealinformation that is invisible to the human eye or traditional imaging techniques. By capturing the subtle nuances of the electromagnetic spectrum, HSI can detect and analyze a diverse range of materials, from minerals and vegetation to man-made objects and even chemical compounds. This capability has made it an indispensable tool in a variety of fields, including remote sensing, environmental monitoring, agriculture, and even medical diagnostics.In the realm of remote sensing, hyperspectral imaging has revolutionized the way we study and manage our natural resources. By analyzing the spectral signatures of different materials, researchers can map and monitor the distribution of minerals, identify areas of vegetation stress, and detect the presence of pollutants or contaminants in the environment. This information is invaluable for a wide range of applications, from mineral exploration and forestry management to environmental impact assessments and disaster response.In the agricultural sector, hyperspectral imaging has become a crucial tool for precision farming and crop monitoring. By analyzing the spectral signatures of plants, farmers can detect early signs of disease, nutrient deficiencies, or water stress, allowing them to take targeted action to improve crop yields and reduce the environmental impact of their operations. Additionally, HSI can be used to map soil composition, monitor crop growth, and even detect the presence ofpests or weeds, enabling more efficient and sustainable farming practices.The medical field has also benefited greatly from the advances in hyperspectral imaging technology. In the area of diagnostics, HSI has shown promise in the early detection of various diseases, such as skin cancer, breast cancer, and cardiovascular conditions. By analyzing the unique spectral signatures of diseased tissues, healthcare professionals can identify subtle changes that may not be visible to the naked eye, enabling earlier intervention and improved patient outcomes.Beyond these applications, hyperspectral imaging has found its way into numerous other industries, including art conservation, forensics, and even aerospace engineering. In the field of art conservation, HSI can be used to identify pigments, detect forgeries, and monitor the condition of valuable artworks, while in forensics, it has been employed to analyze trace evidence and identify illicit substances.As the technology continues to evolve, the potential applications of hyperspectral imaging are virtually limitless. With advancements in sensor technology, data processing, and analytical algorithms, the future of HSI looks increasingly bright, promising new discoveries and innovations that will shape our understanding of the world around us.However, the widespread adoption of hyperspectral imaging technology is not without its challenges. The sheer volume of data generated by HSI systems, coupled with the complexity of the spectral analysis, can pose significant computational and storage challenges. Additionally, the cost of the specialized equipment and the expertise required to interpret the data can be barriers to entry for some organizations and individuals.Despite these challenges, the benefits of hyperspectral imaging are clear, and the technology continues to gain traction across a wide range of industries and disciplines. As researchers and engineers work to overcome the technical hurdles, the future of HSI looks increasingly promising, with the potential to unlock new insights and discoveries that will shape our understanding of the world around us.In conclusion, hyperspectral imaging is a transformative technology that has the power to revolutionize the way we perceive and interact with our environment. By capturing the rich spectral information that lies beyond the visible spectrum, HSI has opened up new frontiers of scientific exploration and practical applications, from remote sensing and precision agriculture to medical diagnostics and forensic analysis. As the technology continues to evolve and become more accessible, the potential of hyperspectral imaging to drive innovation and improve our understanding of the world around us is truly limitless.。

核磁共振基本原理与实验操作指导说明书

核磁共振基本原理与实验操作指导说明书

Chapter 1: NMR Coupling ConstantsNMR can be used for more than simply comparing a product to a literature spectrum. There is a great deal of information that can be learned from analysis of the coupling constants for a compound.1.1Coupling Constants and the Karplus EquationWhen two protons couple to each other, they cause splitting of each other’s peaks. The spacing between the peaks is the same for both protons, and is referred to as the coupling constant or J constant. This number is always given in hertz (Hz), and is determined by the following formula:J Hz = ∆ ppm x instrument frequency∆ ppm is the difference in ppm of two peaks for a given proton. The instrument frequency is determined by the strength of the magnet, and will always be 300 MHz for all spectra collected on the organic teaching lab NMR.Figure 1-1 below shows the simulated NMR spectrum of 1,1-dichloroethane, collected in a 30 MHz instrument. This compound has coupling between A (the quartet at 6 ppm) and B (the doublet at 2 ppm).Figure 1-1: The NMR spectrum of 1,1-dichloroethane, collected in a 30 MHz instrument. For both A and B protons, the peaks are spaced by 0.2 ppm, equal to 6 Hz in this instrument.For both A and B, the distance between the peaks is equal. In this example, the spacing between the peaks is 0.2 ppm (for example, the peaks for A are at 6.2, 6.0, 5.8, and 5.6 ppm). This is equal to a J constant of (0.2 ppm • 30 MHz) = 6 Hz. Since the shifts are given in ppm or parts per million, you should divide by 106. But since the frequency is in megahertz instead of hertz, you should multiply by 106. These two factors cancel each other out, making calculations nice and simple.Figure 1-2 below shows the NMR spectrum of the same compound, but this time collected in a 60 MHz instrument.Chapter 1: NMR Coupling ConstantsFigure 1-2: The NMR spectrum of 1,1-dichloroethane, collected in a 60 MHz instrument. For both A and B protons, the peaks are spaced by 0.1 ppm, equal to 6 Hz in this instrument.This time, the peak spacing is 0.1 ppm. This is equal to a J constant of (0.1 ppm • 60 MHz) = 6 Hz, the same as before. This shows that the J constant for any two particular protons will be the same value in hertz, no matter which instrument is used to measure it.The coupling constant provides valuable information about the structure of a compound. Some typical coupling constants are shown here.Figure 1-3: The coupling constants for some typical pairs of protons.In molecules where the rotation of bonds is constrained (for instance, in double bonds or rings), the coupling constant can provide information about stereochemistry. The Karplus equation describes how the coupling constant between two protons is affected by the dihedral angle between them. The equation follows the general format of J = A + B (cos θ) + C (cos 2θ), with the exact values of A, B and C dependent on several different factors. In general, though, a plot of this equation has the shape shown in Figure 1-4. Coupling constants will usually, but not always, fall into the shaded band on this graph.Figure 1-4: The plot of dihedral angle vs. coupling constant described by the Karplus equation.Chapter 1: NMR Coupling ConstantsThe highest coupling constants will occur between protons that have a dihedral angle of either 0° or 180°, and the lowest coupling constants will occur at 90°. This is due to orbital overlap – when the orbitals are at 90°, there is very little overlap between them, so the hydrogens cannot affect each other’s spins very much (Figure 1-5).Figure 1-5: The best orbital overlap occurs at 180° or 0°, which is why the coupling constant is higher for those angles.1.2 Calculating Coupling Constants in MestreNovaTo calculate coupling constants in MestreNova, there are several options. The easiest one is to use the Multiplet Analysis tool. To do this, go to Analysis → Multiplet Analysis → Manual (or just hit the “J” key). Drag a box around each group of equivalent protons. A purple version of the integral bar will appear below each one, along with a purple box above each one describing its splitting pattern and location in ppm. As with normal integrals, you can right-click the integral bar, select “Edit Multiplet”, and set these integrals to whatever makes sense for that particular structure. For example, in Figure 1-6, each peak is from a single proton so each integral should be about 1.00.Figure 1-6: An example NMR spectrum with multiplet analysis.HH H H HHChapter 1: NMR Coupling ConstantsOnce all peaks are labeled, you can go to Analysis → Multiplet Analysis → Report Multiplets. A text box should appear containing information about the peaks in a highly compressed format. You can then copy and paste this text into your lab report as needed. The spectrum shown above has the following multiplets listed:1H NMR (300 MHz, Chloroform-d) δ 5.14 (d, J = 11.7 Hz, 1H), 4.98 (d, J = 11.7 Hz, 1H), 4.75 (d, J = 3.2 Hz, 1H), 3.37 (d, J = 8.5 Hz, 1H), 3.30 (dd, J = 8.5, 3.3 Hz, 1H).The first set of parentheses indicates that the sample was dissolved in Chloroform-d and placed in a 300 MHz instrument. After that, there is a list of numbers. Each number or range indicates the chemical shift of each of the peaks in the spectrum, in order of descending chemical shift. Each number also has a set of parentheses after it, giving information about that peak. These parentheses contain: • A letter or letters to indicate the splitting of a peak (s=singlet, d=doublet, t=triplet, q=quartet); it is also possible to see things like dd for a doublet of doublets or b for broad. If MestreNova can’t identify a uniform splitting pattern, it will name it a multiplet (m).•The coupling constants or J-values for that peak – for example, the peak at 3.30 ppm has J-values of 8.5 and 3.3 ppm.•The integral of the peak, rounded to the nearest whole number of H.Using this information, you can determine which peaks in Figure 1-6 are coupling to each other based on which ones have matching J-values.•Peaks A and B in Figure 1-6 both have J-values of 11.7 Hz, so these two protons are coupling to each other.•Peaks C and E both have J-values of 3.2 or 3.3 Hz (similar enough, within a margin of error), so these two protons are coupling to each other.•Peaks D and E both have J-values of 8.5 Hz, so these two protons are coupling to each other. If the multiplet analysis tool is failing to determine J-values for any reason, you can always calculate them manually. To do this, you will need to get more precise values for your peak locations. Right-click anywhere in the empty space of the spectrum and select Properties, then go to Peaks and increase the decimals to 4 (Figure 1-7).Chapter 1: NMR Coupling ConstantsFigure 1-7: Changing the decimals on peak labeling.Now if you do peak-picking to label the locations of the peaks, you should see them to 4 decimal places. This will allow you to plus these into the equation to find the J-values manually. For example, in Figure 1-8, the peaks around 4.7 ppm have a J-value of (4.7550 ppm – 4.7442 ppm) • 300 MHz = 3.24 Hz. Note that this in in agreement with MestreNova’s determination of 3.2 ppm for this J-value in Figure1-6.Figure 1-8: Peaks labeled with enough precision to allow you to calculate J-values manually.Chapter 1: NMR Coupling Constants1.3 Topicity and Second-Order CouplingDuring the NMR tutorial, you learned about the concept of chemical equivalence: protons in identical chemical environments have identical chemical shifts. However, just because two protons have the same connectivity to the molecule does not mean they are chemically equivalent. This is related to the concept of topicity : the stereochemical relationship between different groups in a molecule. To find the topicity relationship of two groups to each other, you should try replacing first one group, then the other group with a placeholder atom (in the examples in Figure 1-9, a dark circle is used as the placeholder). If the two molecules produced are identical, then the groups are homotopic; if the molecules are enantiomers, then the groups are enantiotopic; and if the molecules are diastereomers, then the groups are diastereotopic. Groups that are diastereotopic are chemically inequivalent, so they will have a different chemical shift from each other in NMR, and will show coupling as if they were neighboring protons instead of on the same carbon atom.Figure 1-9: Some examples of homotopic, enantiotopic, and diastereotopic groups.If two signals are coupled to each other and have very similar (but not identical) chemical shifts, another effect will appear: second-order coupling. This means that the peaks appear to “lean” toward each other – the peaks on the outside of the coupled pair are shorter, and the peaks on the inside are taller. (Figure 1-10).Figure 1-10: As the chemical shifts of H a and H b become more and more similar, the coupling between them becomes more second-order and the peaks lean more.Chapter 1: NMR Coupling Constants This is very common for two diastereotopic protons on the same carbon atom, but it appears in other situations where two protons are almost chemically identical as well. In Figure 1-8, note the two doublets at 4.98 and 5.14 ppm. These happen to be diastereotopic protons – they are attached to the same carbon, but are chemically equivalent.Looking for pairs of leaning peaks is useful, because it allows you to identify which protons are coupled to each other in a complicated spectrum. In Figure 1-11, there are two different pairs of leaning peaks: two 1H peaks with a J = 9 Hz, and two 2H peaks with J = 15 Hz. Recognizing this makes it possible to pick apart the different components of the peaks towards the left of the spectrum: these are two overlapping doublets, not a quartet.Figure 1-11: An NMR spectrum with two different pairs of leaning peaks.The multiplet tool in MestreNova might not work immediately for analyzing overlapping multiplets like this. Instead, you should follow the instructions at /resolving-overlapped-multiplets/ to deal with them.。

THE MODERATE resolution Imaging Spectroradiometer

THE MODERATE resolution Imaging Spectroradiometer

Analysis of Leaf Area Index and Fraction of PAR Absorbed by Vegetation Products Fromthe Terra MODIS Sensor:2000–2005 Wenze Yang,Dong Huang,Bin Tan,Julienne C.Stroeve,Nikolay V.Shabanov,Yuri Knyazikhin,Ramakrishna R.Nemani,and Ranga B.MyneniAbstract—The analysis of two years of Collection3andfive years of Collection4Terra Moderate Resolution Imaging Spec-troradiometer(MODIS)Leaf Area Index(LAI)and Fraction of Photosynthetically Active Radiation(FPAR)data sets is presented in this article with the goal of understanding product quality with respect to version(Collection3versus4),algorithm(main versus backup),snow(snow-free versus snow on the ground),and cloud(cloud-free versus cloudy)conditions.Retrievals from the main radiative transfer algorithm increased from55%in Collec-tion3to67%in Collection4due to algorithm refinements and improved inputs.Anomalously high LAI/FPAR values observed in Collection3product in some vegetation types were corrected in Collection4.The problem of reflectance saturation and too few main algorithm retrievals in broadleaf forests persisted in Collection4.The spurious seasonality in needleleaf LAI/FPAR fields was traced to fewer reliable input data and retrievals during the boreal winter period.About97%of the snow covered pixels were processed by the backup Normalized Difference Vegetation Index-based algorithm.Similarly,a majority of retrievals under cloudy conditions were obtained from the backup algorithm.For these reasons,the users are advised to consult the qualityflags accompanying the LAI and FPAR product.Index Terms—Evaluation and assessment,Fraction of Photo-synthetically Active Radiation(FPAR)absorbed by vegetation, Leaf Area Index(LAI),Moderate Resolution Imaging Spectrora-diometer(MODIS).I.I NTRODUCTIONT HE MODERATE resolution Imaging Spectroradiometer (MODIS)is an instrument on board NASA’s Terra and Aqua platforms for remote sensing of the Earth’s atmosphere, oceans and land surface.The Terra platform was launched on December18,1999and the Aqua platform on May4,2002.The MODIS instrument has a swath width of2330km,orbit height of705km,and produces global coverage every one to two days. MODIS measures reflected solar and emitted thermal radiation in36spectral bands and at three different spatial resolutions (250,500,and1000m)[1].The MODIS Land team is responsible for the development of algorithms for operationally producing16geophysical data Manuscript received January17,2005;revised October20,2005.This work was supported by the NASA Earth Science Enterprise.W.Yang,D.Huang,B.Tan,N.V.Shabanov,Y.Knyazikhin,and R.B.Myneni are with the Department of Geography,Boston University,Boston,MA02215 USA(e-mail:ywze@).J.C.Stroeve is with the National Snow and Ice Data Center,University of Colorado,Boulder,CO80309USA.R.R.Nemani is with the Ecosystem Science and Technology Branch,NASA Ames Research Center,Moffett Field,CA94035USA.Digital Object Identifier10.1109/TGRS.2006.871214products and their validation.The products include vegetation green leaf area index(LAI)and the fraction of photosynthet-ically active radiation(400–700nm)absorbed by vegetation (FPAR)[2].LAI is defined as the one-sided green leaf area per unit ground area in broadleaf canopies and as half the total needle surface area per unit ground area in coniferous canopies. These products are useful in studies of the exchange offluxes of energy,mass(e.g.,water and CO),and momentum between the surface and atmosphere[3].Research on MODIS LAI and FPAR products is performed along three broad fronts—algorithm development,product anal-ysis,and validation.Algorithm development includes the de-velopment of the at-launch algorithm[4]–[7],prototyping of the algorithm[8],and algorithm refinement[9].Product anal-ysis includes assessment of algorithm performance(this article) and product quality with emphasis on understanding how input data uncertainties constrain LAI/FPAR retrievals[10],[11].Val-idation includes comparison of the product tofield measure-ments scaled to MODIS resolution with the goal of quantita-tively establishing product accuracy,precision,and uncertainty [12]–[18].An article summarizing the validation activities of our team is included in this journal issue[19].MODIS product versions are called Collections.Collection 3is thefirst significant processing of MODIS data into prod-ucts and covered the26month period from November2000 to December2002(96data sets,one for every eight-day pe-riod,totaling157GB).Collection4represents the latest version of MODIS products and contains the entire time series of data starting from February2000to the present(242data sets,one for every eight-day period,totaling396GB).At1-km spatial resolution,the land area constitutes about170million pixels,of which128million are vegetated.This article is based on anal-ysis of the entire Collection3and4Terra MODIS LAI and FPAR data sets—about108billion pixel values.The purpose of the study is to compare the effects of version(Collection3 versus4),input reflectance data quality indirectly through the algorithm(main versus backup),snow(snow-free versus snow on the ground),and cloud(cloud-free versus cloudy)conditions on LAI/FPAR retrievals.II.M ODIS LAI/FPAR A LGORITHM AND P RODUCTSA.Algorithm InputsThe algorithm performs retrievals of LAI and FPAR from daily surface reflectance data at1km resolution.Currently,the0196-2892/$20.00©2006IEEEFig.1.Global maps of LAI,FPAR and Quality Control(QC)generated from Collection3(panels a,c,e)and Collection4(b,d,f)data sets for Julian dates 217–225in year2002(August5–13,2002).red(648nm)and near-infrared(858nm)bands are utilized be-cause of high uncertainties in the other land bands[10].Another important input to the algorithm is the biome classification map, in which the global vegetation is stratified into six canopy archi-tectural types,or biomes[20].The six biomes are:1)grasses and cereal crops,2)shrubs,3)broadleaf crops,4)savannas, 5)broadleaf forests,and6)needle leaf forests.These biomes span structural variations along the horizontal(homogeneous versus heterogeneous)and vertical(single-versus multistory) dimensions,canopy height,leaf type,soil brightness,and cli-mate(precipitation and temperature)space of herbaceous and woody vegetation.The biome map reduces the number of un-knowns of the inverse problem through the use of simplifying assumptions(e.g.,model leaf normal orientation distributions) and standard constants(e.g.,leaf,wood,litter,and soil optical properties)that are assumed to vary with biome and soil types only.This approach is similar to that adopted in many global models which assume certain key parameters to vary only by vegetation type and utilize a land cover classification to achieve biome specific parameterization.B.AlgorithmThe retrievals are performed with the radiative transfer algo-rithm,also called the main algorithm hereafter[4],[5].The al-gorithmfinds LAI and FPAR values given sun and view direc-tions,Bidirectional Reflectance Factor(BRF)for each MODIS band,band uncertainties,and six biome land cover classes.The retrieval technique compares observed and modeled BRFs for a suite of canopy structures and soil patterns that represent an expected range of typical conditions for a given biome type. All canopy/soil patterns for which modeled and observed BRFs differ within a specified uncertainties level are considered as ac-ceptable solutions.The mean values of LAI averaged over all acceptable solutions are reported as the output of the algorithm. This physically-based LAI and FPAR algorithm was developedYANG et al.:ANALYSIS OF LEAF AREA INDEX AND FRACTION OF PAR1831TABLE II NTERPRETATION OF THE C OLLECTION T HREE Q UALITY F LAGS A CCOMPANYING THE T ERRA M ODIS LAI AND FPAR PRODUCTSfor operational use with MODIS data.The main algorithm re-trievals,therefore,are the MODIS standard product.The algo-rithm,however,may fail if input reflectance data uncertainties are greater than preset threshold values in the algorithm or due to deficiencies in model formulation which result in incorrect simulated BRFs.In all such cases,the retrievals are generated by a backup algorithm based on biome-specific empirical rela-tionships between the Normalized Difference Vegetation Index (NDVI)and LAI/FPAR[21].Additional details on algorithm physics can be found in[6],[7],[10],and[22].I and FPAR ProductsThe products are produced at1-km spatial resolution daily and composited over an eight-day period based on the max-imum FPAR value.The eight-day product is distributed to the public from the EROS Data Center Distributed Active Archive Center[WWW1].Collection3represents thefirst significant processing of MODIS data into products after various initial problems with instrument calibration and electronics have been resolved,and covers the26-month period from November2000 to December2002.This Collection,thus,provided an oppor-tunity for evaluating the initial batch of products from Terra MODIS.These efforts lead to algorithm refinements which were implemented in Collection4processing that started in January 2003.Collection4represents the latest version of MODIS prod-ucts and contains the entire time series of data starting from Feb-ruary2000to the present.MODIS products are projected on the Integerized Sinusoidal (Collection3)and the Sinusoidal(Collection4)ten-degree grids,where the globe is tiled into36tiles along the east-west axis,and18tiles along the north-south axis[23].Both Col-lection3and4MODIS LAI and FPAR products have been stage-1validated[WWW2],that is,product accuracy has been estimated using a small number of independent measurements from selected locations and time periods through ground-truth and validation efforts[19].The productfiles contain qualityflags in addition to LAI and FPARfields.The qualityflag bit-fields provide information about the overall quality of the product,algorithm path,cloud state,aerosols,snow,etc.(Tables I and II).The users are advised to use the quality control variables to select reliable retrievals. Examples of global maps of LAI,FPAR and quality control vari-ables for Collection3and4products are shown in Fig.1for composite days August5–13,2002.D.Changes in Collection ProcessingThe Collection4processing of LAI and FPAR products bene-fited from improved inputs(surface reflectance data and biome1832IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING,VOL.44,NO.7,JULY2006TABLE III NTERPRETATION OF THE C OLLECTION F OUR Q UALITY F LAGS A CCOMPANYING THE T ERRA M ODIS LAI AND FPAR PRODUCTSmap)and algorithm physics(LUTs and compositing),as dis-cussed below.Refinements to atmospheric correction algorithm resulted in a significant increase in the spatial extent of high quality surface reflectance data.This directly impacted the spa-tial extent of the LAI and FPAR product(Fig.1).The number of backup algorithm retrievals decreased from Collection3to4, but this meant more main algorithm retrievals under saturation in broadleaf forests.The Advanced Very High-Resolution Ra-diometer(A VHRR)data-based biome map used in Collection 3processing had high uncertainties compared to the MODIS data-based biome map used in Collection4[20].The two maps are shown in Fig.2and compared in Table III.The Collection3 biome map had significant misclassification of grasses and ce-real crop pixels into broadleaf crop pixels which impacted LAI and FPAR retrievals.The quality of retrievals was especially low in the case of misclassification between forest and nonforest vegetation classes[8],[23].The Collection3algorithm look-up tables(LUTs)were based on surface reflectance data from the Sea-Viewing Wide Field-of-view Sensor(SeaWiFS)sensor[10]while the Collec-tion4LUTs were based on MODIS surface reflectance data. LAI histograms for the six biomes shown in Fig.3illustrate the impact of LUTs on LAI retrievals.The main problem with Collection3product was LAI overestimation in thefirst four biome types(Section II-A).This was also ascertained through validation activities[24],[19].The problem was traced to mismatch between simulated reflectances evaluated from LUT entries and MODIS reflectances,thus resulting in incorrect LAI/FPAR retrievals or algorithm failure[11].The LUTs were, therefore,revised according to the process described in[4],[8], and[9].The compositing scheme of the algorithm was revised in Col-lection4processing.Amongst the set of LAI/FPAR values from the eight-day compositing period,the LAI/FPAR pair corre-sponding to the maximum FPAR value was selected,irrespec-tive of algorithm path,to represent the eight-day composited MODIS LAI product in Collections1–3.This scheme lead to poor quality compositing results when backup retrievals number more than the main algorithm retrievals in the eight-day pe-riod because the backup algorithm retrievals are inherently de-rived from lower quality inputs.This compositing scheme was changed in Collection4to select the LAI/FPAR pair corre-sponding to the maximum FPAR value generated by the main algorithm.The backup algorithm retrievals are selected onlyYANG et al.:ANALYSIS OF LEAF AREA INDEX AND FRACTION OF PAR1833parison of biome maps used in Collection 3(panel a)and 4(panel b)processing.The relative proportions are shown in panels c and d.A detailed comparison is presented in Table III.TABLE IIIE RROR M ATIXF ROM C OMPARISON OF B IOME M APS U SED IN C OLLECTIONS 3AND 4LAI/FPAR PROCESSINGwhen no main algorithm retrievals are available during the com-positing period.III.R ESULTS AND D ISCUSSIONA.Retrieval IndexThe retrieval index (RI)is de fined as the ratio of the number of pixels with LAI and FPAR retrieved by the main algorithm to the total number of retrievals by both the main and backup algo-rithms.This index does not indicate retrieval quality but rather the success rate of the main algorithm.The retrieval index shows a stable but seasonal pattern through the five years of MODIS operations [Fig.4(a)].The annual average retrieval index in-creased from 55%in Collection 3to 67%in Collection 4be-cause of the changes discussed in Section II-D.The retrieval index can be as low as 40%–50%during the boreal winter time mainly due to poor quality surface re flectances and as high as 65%–80%during the boreal summer time.The points shown off the line in Fig.4(a)are due to a pause in data collection by the Terra MODIS instrument [WWW3].Grasses and cereal crops have the highest retrievals from the main algorithm (50%in winter and 80%–90%in summer)when retrievals are analyzed by biome type [Fig.4(b)].The retrieval rate is lowest in the case of broadleaf forests (40%through the year)because of re flectance saturation in dense canopies,that is,the re flectances do not contain suf ficient information to localize a LAI value [4],[5].Changes planned for Collection 5processing are aimed at improving the retrieval rate in dense forests [9].The seasonality in retrieval rate is most pronounced in the high northern latitudes [Fig.4(c)].The few main algorithm retrievals during the winter time in this region are due to snow and/or cloudy conditions and fewer measurements (surface re flectances are not generated for solarzenithangle).The retrieval index for this region is as high as 80%during the summer time.Overall,the retrieval rates of the main algorithm increased by 8%–16%in all latitudes in1834IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING,VOL.44,NO.7,JULY2006I histograms from Collection3(dashed line)and4(solid line)by biome type and algorithm path.the Collection4processing.Table IV summarizes the overall effect of version(Collection3versus4)on LAI/FPAR retrievals from the main algorithm.I and FPAR FieldsThe time series of LAI and FPARfields from the Terra MODIS sensor exhibit the seasonal cycle as expected,with a boreal winter time minimum of about1.5(1.3)and a boreal summer time maximum of about2.5(1.8)for the Collection3 (Collection4)processing[Fig.5(a)and(b)].The difference between the two Collections is due to two reasons—1)LAI overestimation in thefirst four biomes(Section II-A)stemming from a mismatch between simulated reflectances evaluated from algorithm LUT entries and MODIS reflectances in Col-lection3,and2)significant misclassification of grasses and cereal crop pixels into broadleaf crop pixels in the biome map used in Collection3processing.The small random variations in the LAI time series are due to variations in data availability related to cloud cover rather than phenological changes.The global FPAR time series also shows similar seasonality,varying from about0.45in winter to0.55in summer.Thus,on average, about50%of the incident photosynthetically active radiation is absorbed by vegetation.This high rate of absorption is possible because the vegetated area is magnified by a factor of1.3to1.8 through layering of leaves in the canopy.The LAI and FPAR profiles derived from the main algorithm for individual biomes are shown in Fig.5(c)and(d).The am-plitude of seasonal variations for a particular biome type at the global scale may be lower or different than the corresponding amplitude at regional scales.The Southern and Northern hemi-YANG et al.:ANALYSIS OF LEAF AREA INDEX AND FRACTION OF PAR1835Fig.4.Time series of the main algorithm retrieval rate,denoted here as the retrieval index(fraction of main algorithm retrievals)for Collection3(dashed lines) and4(solid lines)data sets.The global retrieval index is shown in panel a,the points shown off the line are due to a pause in data collection by the Terra MODIS instrument[WWW3].The retrieval index for different biomes(latitudinal bands)is shown in panel b(panel c).The abbreviations C3and C4refer to Collection3 and4products,respectively.TABLE IVT EMPORALLY AND S PATIALLY A VERAGED R ETRIEV AL I NDEX FOR S IX B IOMES IN C OLLECTIONS3AND4LAI/FPAR PROCESSINGspheres have opposing growing seasons which dampen the sea-sonal amplitude at the global scale.Grasses and cereal crops have LAI values of about1through the year with negligible seasonal variations.Broadleaf forests indicate some seasonality with LAI varying from about4during the boreal winter to5.5in the summer.This biome class includes both evergreen broadleaf forests from the tropics and deciduous broadleaf forests from the temperate regions.A stronger seasonality is seen in needleleaf forests with LAI varying from about2during the boreal winter to4.5in the summer.Some of this seasonality is an artifact re-sulting from low data availability during the boreal winter time due to weak illumination conditions,extreme solar zenith an-gles,snow and cloud contamination.The zonal mean LAI value from the tropics is about1.5in Collection4which may seem low[Fig.5(e)and(f)].In this band,large areas are under savanna(33%,),shrubs(17%,),and grasses(10%,).The ever-green broadleaf forests occupy34%of the area in the tropics (23S–23N)with a mean LAI value of about4.2.In the higher northern latitudes(N),the summer time LAI values are about2.5in Collection4.Low winter time LAI values here are an artifact due to snow and/or cloudy conditions,and the low availability of surface reflectances data.This will be further dis-cussed in Sections III-D and III-E.C.Main and Backup Algorithm RetrievalsThe foregoing analysis indicates that the main algorithm suc-cess rate is about70%in Collection4processing.The main1836IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING,VOL.44,NO.7,JULY2006I and FPAR from Collection3(dashed lines)and4(solid lines)data sets.The global retrieval index is shown in panels a and b.The averaged annual profile of LAI and FPAR for different biomes(latitudinal bands)is shown in panels c and d(panels e and f).The abbreviations C3and C4refer to Collection3and 4products,respectively.(a)MOD15A2,Main Algorithm.(b)MOD15A2,Main Algorithm.(c)MOD15A2,Main Algorithm.(d)MOD15A2,Main Algorithm.(e)MOD15A2,Main Algorithm.(f)MOD15A2,Main Algorithm.algorithm failure in30%of the pixels may be due to uncer-tainties in input surface reflectance data greater than threshold values preset in the algorithm,biome misclassifications and al-gorithm deficiencies that can result in an incorrect or no match between simulated reflectances evaluated from LUT entries and MODIS reflectances.The backup NDVI based algorithm trig-gered in these instances is insensitive to input data uncertainties and always provides a retrieval if NDVI is a positive number. The quality of retrievals from the backup algorithm is,thus,gen-erally poor.The following analysis was performed to compare main and backup algorithm retrievals.The difference between the main and backup algorithms re-trievals is defined as delta LAI(delta FPAR).Delta LAI is cal-culated at a monthly scale as a function of both biome type and latitudinal band.The four successive eight-day LAI values of a pixel in one month areflagged for further analysis if this se-quence contains retrievals from both the main and backup algo-rithms.The mean main algorithmLAI,,and the meanYANG et al.:ANALYSIS OF LEAF AREA INDEX AND FRACTION OF PAR1837Fig.6.Difference between main and backup algorithm retrievals in Collection4(solid lines).Results are shown for three representative biomes(grasses and cereal crops,savannas,and broadleaf forests),two-month(August and March)and two-latitudinal bands(north of40N and23S to23N).Also shown are the histograms of LAI and FPAR values retrieved by the main algorithm(dotted lines).(a)Grasses and Cereal Crops.(b)Grasses and Cereal Crops.(c)Savannas.(d)Savannas.(e)Broadleaf Forests.(f)Broadleaf Forests.backup algorithmLAI,,are used to evaluate deltaLAI of thepixel,.Similarcalculations are performed for FPAR data.Representative delta LAI and FPARfields evaluated with the Collection4data are shown in Fig.6for three biome types (grasses and cereal crops,savannas,and broadleaf forests)in two broad latitude bands(north of40N and23S–23N)during two representative months(August and March).Delta LAI and FPAR are generally linear with respect to the mean main algo-rithm LAI and FPAR values.Delta FPAR is less than0.2in two of the three biomes studied(Fig.6)over the range of the most probable FPAR values(0.4–0.8in grasses and cereal crops and 0.8–0.9for broadleaf forests).However,delta FPAR is signifi-cantly higher(about0.4)in savannas,where the most probable FPAR values range from0.6to0.95.The same is true of delta LAI.Grasses and cereal crops have a low LAI values(0.5–2.0) and delta LAI is close to zero for a majority of pixels.Broadleaf forests have high LAI values(4.0–6.5)and delta LAI is also high—about1.8in the northern latitudes during August and3.4 in the tropical band during March.The above-mentioned differences in LAI and FPAR values from the main and backup algorithms highlight certain limita-tions of the backup algorithm.Recall that the backup algorithm is based on biome-specific relations between NDVI and LAI (FPAR).These relations,although based onfield data and model calculations,are,nevertheless,site-specific and can not account1838IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING,VOL.44,NO.7,JULY2006Fig.7.Impact of snow on LAI/FPAR retrievals over vegetated areas north of40N.The annual course of the percentage of snow covered pixels in Collection3 (panel a)and4(panel b)data.The annual course of the pixel counts and total LAI(the sum of LAIs over corresponding pixels)retrieved by the Collection4main and backup algorithms under various snow conditions is shown for broadleaf forests(panels c and e)and needleleaf forests(panels d and f).The abbreviations C3 and C4refer to Collection3and4products,respectively.for natural variability of vegetation.Moreover,an important reason why the main algorithm fails is cloud and snow con-tamination of surface reflectances.The backup algorithm will retrieve low LAI and FPAR values as it is insensitive to input uncertainties(Sections III-D and III-E).This analysis highlights the need for examining the product qualityflags accompanying the product(Tables I and II).D.Retrievals From Pixels With SnowThe impact of snow cover on algorithm performance is ex-amined here to understand the seasonality observed in LAI and FPARfields in the boreal zones[Fig.5(e)and(f)].Informa-tion on snow cover of each pixel is contained in the product qualityflags accompanying the products(Tables I and II).Ac-cording to this information,about20%to30%of the vegetated pixels north of40N are identified as having snow during the peak winter months[Fig.7(a)and(b)].Under such conditions, the main algorithm retrieval rate is1.4%in Collection3and 2.5%in Collection4.The high failure rate is due to the fact that snow significantly increases both RED and NIR reflectances, such that NDVI is close to0[25],and the reflectances are not part of the retrieval domain of the main algorithm.The backupFig.8.Impact of clouds on LAI/FPAR retrievals.Three cloud conditions were used—cloudy,partially cloudy,and clear.The annual course of the percentage of pixels retrieved by the main or the backup algorithms under various cloud conditions in Collection3(panel a)and4(panel b)data.The annual course of LAI retrieved by the Collection4main and backup algorithms under different cloud conditions is shown in panel c for grasses and cereal crops,in panel d for broadleaf forests and in panel e for needleleaf forests.(a)Collection3.(b)Collection4.(c)Grasses and Cereal Crops.25.4%Vegetated Pixels,Northern Hemisphere.(d)Droadleaf Forests,17.7%Vegetated,Pixels,Global.(e)Needleleaf Forests,12.0%Vegetated Pixels,Global.NDVI-based algorithm invoked in these cases provides LAI andFPAR retrievals close to0.Such pixels are tagged as having poorquality in the QAfields(Tables I and II).The Collection4LAI retrievals under various snow con-ditions in the high northern latitudes(N)are shown inFig.7(c)–(f),separately for broadleaf and needleleaf forests. Total LAI(the sum of LAI values over corresponding pixels),instead of average LAI,is shown in Fig.7(e)and(f).The total LAI under snow-free conditions is about100times greater than that with snow,that is,LAI retrievals under snow conditions are a very small portion of the total retrievals,generally speaking. During the winter months,however,retrievals under snow conditions are considerable,and,in fact,there are too few main algorithm retrievals to reliably estimate average LAI valuesfor needleleaf forest pixels[Fig.7(d)].Therefore,the LAI and FPAR seasonality in needleleaf forests,seen in Fig.5(c)–(d), is spurious and must be treated as an artifact resulting from too few reliable retrievals.It should also be noted that there are few reliable measurements in the high northern latitudes during the winter period because of low sun angles and weak illumination conditions,which further amplify the problem of reliably estimating LAI and FPAR in these regions during the winter months.E.Retrievals Under Cloudy ConditionsThe MODIS cloud screening algorithm uses as many as14 of the MODIS36spectral bands to maximize the reliability of cloud rmation on cloud optical thickness,effec-tive radius,cloud-top properties is also used to determine the cloud mask(e.g.,single layer,multilayer,clear sky,etc.).De-tails on the MODIS cloud mask are described in[26]. Information on pixel cloud state is contained in qualityflags accompanying the products(Tables I and II).Note that the LAI/FPAR algorithm is executed irrespective of cloud state. About50%to65%of the vegetated pixels are identified as cloud free,15%as partially cloudy and the rest as cloud covered in the Collection3data set[Fig.8(a)].In Collection4,about 65%to75%of the vegetated pixels are identified as cloud free, 10%as partially cloudy and the rest as cloud covered[Fig. 8(b)].These differences must be attributed to refinements in cloud screening algorithm.The increase in the amount of cloud free pixels in Collection4is especially noticeable during the boreal winter time period[Fig.8(a)and(b)].The annual course of Collection4LAI retrieved by the main and backup algorithms under different cloud conditions is shown in Fig.8(c)–(e)for three example biomes(grasses and cereal crops,broadleaf forests,and needleleaf forests).The majority of retrievals under cloudy conditions are performed with the backup algorithm.Main algorithm failure in such cases is expected as the input reflectance data have large uncer-tainties.The main algorithm does not fail in some limited cases of cloudiness[cf.Fig.8(b)].This situation may correspond to the case of cloud cover overestimation by the cloud screening algorithm.The results shown in Fig.8suggest that the annual course of LAI values retrieved by the main algorithm shows similar patterns,irrespective of the degree of cloudiness.How-ever,the differences in LAI magnitudes between the retrievals under cloud-free and cloudy conditions are not negligible,and they depend on the biome type and the time of the year.This again reinforces the need for examining the product quality flags accompanying the LAI and FPAR product(Tables I and II).IV.C ONCLUSIONThe following conclusions can be drawn based on the analysis of two years of Collection3andfive years of Collection4Terra MODIS LAI and FPAR data sets.The success rate of the main radiative transfer algorithm increased from55%in Collection 3to67%in Collection4.This is due to the new LUTs imple-mented in Collection4and also due to refinements to upstream algorithms that provide inputs to the LAI/FPAR algorithm.The time series of LAI and FPARfields exhibit the seasonal cycle with a boreal winter time minimum of about1.5(1.3)and a boreal summer time maximum of about2.5(1.8)for the Col-lection3(Collection4)processing.This difference between the two Collections is due to two reasons—1)LAI overestimation in thefirst four biomes(Section II-A)stemming from a mis-match between simulated reflectances evaluated from algorithm LUT entries and measured MODIS reflectances in Collection3, and2)significant misclassification of grasses and cereal crop pixels into broadleaf crop pixels in the biome map used in Col-lection3processing.Less than2%–3%of the pixels tagged as covered with snow are processed by the main algorithm.Thus, most of the snow covered pixels are processed by the backup NDVI-based algorithm which is insensitive to input reflectance data quality.Similarly,a majority of retrievals under cloudy conditions are obtained from the backup algorithm.For these reasons,the backup algorithm retrievals have low quality and should not be used for validation and other studies.The users are advised to consult the qualityflags accompanying the LAI and FPAR product to select high quality retrievals.The analysis presented here demonstrates the physical basis of the main algorithm used to generate the MODIS standard LAI and FPAR product,and importantly,that the reliability and spa-tial coverage of the retrievals increase with increased input ac-curacy.Further improvements in the quality of MODIS surface reflectances and biome map are,therefore,expected to lead to LAI and FPAR product of increasing quality and coverage.The role of the backup algorithm will be consequently reduced.A PPENDIX IWWW S ITESWWW1:EROS Data Center Distributed Active Archive Center,/pub/im-swelcome/WWW2:The EOS Land Validation Home Page, /WWW3:Terra MODIS Instrument Performance History,/mcstweb/per-formance/terra_instrument.html.A CKNOWLEDGMENTThe authors would like to thank the many individuals who contributed to the successful and timely operational processing of MODIS data,especially Dr.El Saleous,Dr.Friedl,Dr.Jus-tice,Dr.Roy,Dr.Vermote,and and Dr.Wolfe for their effort and support.R EFERENCES[1]W.L.Barnes,T.S.Pagano,and V.V.Salomonson,“Prelaunch character-istics of the moderate resolution imaging spectroradiometer(MODIS) on EOS-AM1,”IEEE Trans.Geosci.Remote Sens.,vol.36,no.4,pp.1088–1100,Jul.1998.[2] C.O.Justice,J.R.G.Townshend,E.F.Vermote,E.Masuoka,R.E.Wolfe,N.Saleous,D.P.Roy,and J.T.Morisette,“An overview of MODIS land data processing and product status,”Remote Sens.Env-iron.,vol.83,pp.3–15,2002.[3]P.J.Sellers,R.E.Dickinson,D.A.Randall,A.K.Betts,F.G.Hall,J.A.Berry,G.J.Collatz,A.S.Denning,H.A.Mooney,C.A.Nobre,N.Sato,C.B.Field,and A.Henderson-Sellers,“Modeling the exchanges of energy,water,and carbon between continents and the atmosphere,”Science,vol.275,pp.502–509,1997.。

近红外光谱法英文

近红外光谱法英文

近红外光谱法英文Near-Infrared SpectroscopyNear-infrared spectroscopy (NIRS) is a powerful analytical technique that has gained widespread recognition in various scientific and industrial fields. This non-invasive method utilizes the near-infrared region of the electromagnetic spectrum, typically ranging from 700 to 2500 nanometers (nm), to obtain valuable information about the chemical and physical properties of materials. The versatility of NIRS has led to its application in a diverse array of industries, including agriculture, pharmaceuticals, food processing, and environmental monitoring.One of the primary advantages of NIRS is its ability to provide rapid and accurate analysis without the need for extensive sample preparation. Unlike traditional analytical methods, which often require complex sample extraction and processing, NIRS can analyze samples in their natural state, allowing for real-time monitoring and decision-making. This efficiency and non-destructive nature make NIRS an attractive choice for applications where speed and preservation of sample integrity are crucial.In the field of agriculture, NIRS has become an invaluable tool for the assessment of crop quality and the optimization of farming practices. By analyzing the near-infrared spectra of plant materials, researchers can determine the content of various nutrients, such as protein, carbohydrates, and moisture, as well as the presence of contaminants or adulterants. This information can be used to guide precision farming techniques, optimize fertilizer application, and ensure the quality and safety of agricultural products.The pharmaceutical industry has also embraced the use of NIRS for a wide range of applications. In drug development, NIRS can be used to monitor the manufacturing process, ensuring the consistent quality and purity of active pharmaceutical ingredients (APIs) and finished products. Additionally, NIRS can be employed in the analysis of tablet coatings, the detection of counterfeit drugs, and the evaluation of drug stability during storage.The food processing industry has been another significant beneficiary of NIRS technology. By analyzing the near-infrared spectra of food samples, manufacturers can assess parameters such as fat, protein, and moisture content, as well as the presence of adulterants or contaminants. This information is crucial for ensuring product quality, optimizing production processes, and meeting regulatory standards. NIRS has been particularly useful in the analysis of dairy products, grains, and meat, where rapid and non-destructive testing is highly desirable.In the field of environmental monitoring, NIRS has found applications in the analysis of soil and water samples. By examining the near-infrared spectra of these materials, researchers can obtain information about the presence and concentration of various organic and inorganic compounds, including pollutants, nutrients, and heavy metals. This knowledge can be used to inform decision-making in areas such as soil management, water treatment, and environmental remediation.The success of NIRS in these diverse applications can be attributed to several key factors. Firstly, the near-infrared region of the electromagnetic spectrum is sensitive to a wide range of molecular vibrations, allowing for the detection and quantification of a variety of chemical compounds. Additionally, the ability of NIRS to analyze samples non-destructively and with minimal sample preparation has made it an attractive choice for in-situ and real-time monitoring applications.Furthermore, the development of advanced data analysis techniques, such as multivariate analysis and chemometrics, has significantly enhanced the capabilities of NIRS. These methods enable the extraction of meaningful information from the complex near-infrared spectra, allowing for the accurate prediction of sample propertiesand the identification of subtle chemical and physical changes.As technology continues to evolve, the future of NIRS looks increasingly promising. Advancements in sensor design, data processing algorithms, and portable instrumentation are expected to expand the reach of this analytical technique, making it more accessible and applicable across a wider range of industries and research fields.In conclusion, near-infrared spectroscopy is a versatile and powerful analytical tool that has transformed the way we approach various scientific and industrial challenges. Its ability to provide rapid, non-invasive, and accurate analysis has made it an indispensable technology in fields ranging from agriculture and pharmaceuticals to food processing and environmental monitoring. As the field of NIRS continues to evolve, it is poised to play an increasingly crucial role in driving innovation and advancing our understanding of the world around us.。

kbr压片法测红外光谱英文

kbr压片法测红外光谱英文

kbr压片法测红外光谱英文When it comes to measuring infrared spectra, the KBr pellet method is a commonly used technique. It's pretty straightforward and effective. Basically, you mix your sample with a fine powder of potassium bromide (KBr) and then compress it into a transparent disk or "pellet". The KBr acts as a diluent and helps distribute the sample evenly.The key to getting good results is having a finely ground sample and using the right amount of KBr. Too much or too little can affect the quality of the spectrum. Once you've got your mixture ready, you use a special press to compress it into a pellet.Measuring the infrared spectrum of the pellet is then done using an infrared spectrometer. The instrument shines infrared light through the pellet and measures how much of each wavelength is absorbed. This gives you a unique "fingerprint" of your sample's chemical composition.One of the advantages of the KBr pellet method is that it works well for a wide range of samples. Whether you're dealing with solids, liquids, or even gases, you can often adapt the technique to get the data you need. Plus, the pellets are easy to handle and store, making it a convenient choice for labs of all sizes.In summary, the KBr pellet method is a solid way to measure infrared spectra. With the right tools and technique, you can get reliable and accurate results in no time.。

赛默飞世尔-LS-55和LS-45荧光光谱仪-产品手册说明书

赛默飞世尔-LS-55和LS-45荧光光谱仪-产品手册说明书

Fluorescence Spectroscopy FactsFor bioresearch applications involving analysis of minute quantities of material, few other techniques offer as much sensitivity and selectivity at such a moderate cost.Consider these key advantages:Extraordinary sensitivity:Fluorescence spectroscopy detects concentrations as low as one part per trillion. This is 10,000 times more sensitive than absorption spectroscopy.Because luminescence is measured directly, a small emission can be amplified many times with little noise.Unprecedented selectivity:Scanning fluorescencespectrometers make use of two selectable wavelengths (excitation and emission) for unmatched selectivity and multi-dimensional information. Environmentally sensitive:Electronic transitions measured in fluorescence spectroscopy are longer-lived than those measured in absorption spectroscopy. They also are sensitive to local polarity,pH, viscosity, and thermal effects. Broad-based applications capabilities:Fluorescence instruments have a six- to seven-fold greater linear measurement range for quantitative analysis than spectrophotometers –with little error at the extremes.Safety:Using fluorescent tags instead of radioisotopes simplifies laboratory safety procedures.2Always the right choiceThe PerkinElmer LS-55 and LS-45 FluorescenceSpectrometers provide your laboratory with the ultimate blend of high performance, reliability, ease-of-use, durability,and versatility. The LS-55 and LS-45 are the right choice to meet your laboratory’s current and future applications needs.Indispensable features of these instruments include: Ultimate flexibility.No instruments handle a wider range of bioscience applications than the LS-55 and LS-45. These instruments are ideally suited for bioresearch including cell-biology, immunology, enzymology, protein analysis, and microplate-based measurements.Broad range of accessories.The wide range of accessories specifically designed for the LS-55 and LS-45 is versatile enough to handle virtually any type of sample.Reliable optical performance.The LS-55 features continuously variable slits, in increments of 0.1 nm, for ultimate control of measurement conditions.The holographic gratings of the LS-55 minimize stray light for the highest sensitivity,accuracy and reproducibility. The LS-45 has fixed slits, for sturdy,dependable daily use.Durable, long-lived light source.Unlike conventional light sources, the Pulsed Xenon lamp minimizes photobleaching of samples. This preserves the integrity of the sample and delivers accurate and uncompromised results.Simply Versatile:The LS-55and LS-45Fluorescence SpectrometersThe Pulsed Xenon lamp:Astroke of brilliance Preserving the integrity of your samples is crucial to the success of your experiments. PerkinElmer uses ahigh-energy Pulsed Xenon source for the LS-55 and LS-45. Pulsed Xenon offers these advantages:•Minimal photobleaching of samples •Long-lived excitation for stability and accuracy •Improved low-light detection capability relative to other light sources •Wide UV output (to 200 nm), for great flexibility when selecting excitation wavelengths •Replaceable pre-aligned bulb (no need to replace the entire lamp module)•Delay and gate time can be varied to measure phosphorescence •The ability to be turned off, for measuring chemiluminescence and bioluminescence•Used by thousands of researchers for thousands of applications around the worldThe Pulsed Xenon lamp:A stroke of brilliance Preserving the integrity of your samples is crucial to the success of your experiments. PerkinElmer uses a high-energy Pulsed Xenon source for the LS-55 and LS-45. Pulsed Xenon offers these advantages:•Minimal photobleaching of samples •Long-lived excitation for stability and accuracy •Improved low-light detection capability relative to other light sources •Wide UV output (to 200 nm), for greater flexibility when selecting excitation wavelengths •Replaceable pre-aligned bulb (no need to replace the entire lamp module)•Delay and gate time can be varied to measure phosphorescence •The ability to be turned off, for measuring chemiluminescence and bioluminescence •Used by thousands of researchers for thousands of applications around the worldAcademicCell biology, cellular toxicity/viability, molecular toxicity, proteinfolding/unfolding, receptor binding studies, teaching (simple assays, DNS, and protein quantification), research (assay development, cell-based work), apoptosis studies, and phagocytosis/oxidative processesAgriculturalPesticide tracing, resistance assays, chlorophyll determinations, crop protection,genetic modification, and plant geneticsCell biologyCytotoxicity, cell studies (viability, quantification, proliferation, adhesion), reporter gene, and apoptosisClinicalAssays (enzyme, substrate, toxicity), porphyrins, steroids, vitamin, and amine studiesEnvironmentalPesticide detection, ground water tracing, oil contamination of water, chlorophyll determination of toxic algae, exhaust gas composition, water purity, uranium and aluminum determination, and biomassEnzymologyProtease assays, inhibition studies, surfactant studies, and kinetic assaysImmunologyFluorescent enzyme-linked immunosorbent assays, cell proliferation/activation, and tumor necrosis factorIndustrialCrack tracing (aerospace), security inks, coding phosphors, brightening/whitening reagents, ultraviolet stabilizers and plasticizers, crude oil fingerprinting, and postage stamp markingInorganicAluminum, lead, magnesium, manganese, selenium, zinc, and tinMedicalEnzyme/substrate assays, cellular analysis, including bioluminescence and cell proliferation/activation, immunology assays (tumor necrosis factor and enzyme-linked immunosorbent (ELISA) assayMolecular biologyDNA and mRNA quantification, gene expression, polymerase chain reaction product quantitation, protein quantitation and folding/unfolding, enzyme activity, porphyrin induction assays, and high throughput drug screeningPharmaceuticalVitamins, biogenic amines, drugs, substance abuse, gene expressionand discovery, novel drug delivery systems, combinatorial drug discovery, membrane structure studies, toxicity assays, and cell function assays4The LS-55and LS-45FluorescenceSpectrometers:the industry standardfor bioscience/pharmaceutical applications5Cell ViabilityThe LS-55 can be used to measure cell viability with excellent sensitivity. In this example, the signal from the SYTOX Green nucleic acid stain is proportional to the number of cells thathave lost membrane integrity.Nucleic Acid QuantitationQuantitation of nucleic acid is a routine but essential technique employed by many laboratories. Shown here is the OliGreen assay, which is rapid, sensitive and accurate, with minimalinterference from short oligonucleotides, salts and detergents.EnzymologyExcellent temperature control and the ability to measuresimultaneous reactions make the LS-55 an ideal tool for enzyme studies, such as monitoring the hydrolysis of labeled casein bytrypsin and its inhibition by Aprotinin.Protein QuantitationThe anionic dye sulforhodamine 101 measures total protein by forming complexes with basic amino acid residues. The LS-55 is the perfect choice for using this technique to detect changes in cellsize or population over a range of 100 to more than 100,000 cells.Membrane StructureThe advanced automated polarization capabilities of the LS-55allow studies such as this example of cell membrane fluidity. A fluorescent membrane dye, along with the AutoPole software module and a programmable water bath, was used to measureanisotropy as a function of temperature.Intracellular BiochemistryNon-destructive determinations of intracellular calcium are carried out in living cells by binding with a fluorescent dye.In the presence of a cellular stimulus the LS-55’s fast-filter accessory measures the relative amounts of the free (380 nm)and bound (340 nm) dyes in real-time.Answering the demands of diverse applicationsThe LS-55 and LS-45 Fluorescence Spectrometers feature anentire spectrum of accessoriesWhether your sample is liquid or solid, abundant or limited, there’s an accessory that gets the job done. And, since all accessories are front mounted and easily accessible,you can change sampling modes in an instant.APPLICATION:FEATURES: LS-55LS-4567Additionally, PerkinElmer offers a range of plug-in application modules offering further research capabilities. These include:• Short phosphorescence decay routine • Enzyme-activity program• WPR Scan – allowing the Well-PlateReader to scan multiple spectra per well • Autopol – for programmed measurement of intensity, polarization,or anisotropy vs. temperature• A variety of advanced three-dimensional data-handling, presentation, and export routinesRelax… FL WinLabsoftware is reliable anduser friendlyThe FL WinLab software is user friendly and application specific, seamlessly combining PerkinElmer’s extensive application-specific knowledge and instrument control. The FL WinLab software is easy to configure for the applications you need.Access specific modes of instrument operation –Scan, Time Drive, and Ratio Data Collection –directly from the Applications Menu. You can scan the excitation and emission monochromators either independently or synchronously.FL WinLab software includes a validation protocol that automatically checks instrument performance.This ensures reliable data and helps your laboratory comply with good laboratory practice (GLP).P e r k i n E l m e r F F l u o r e s c e n c e S S p e c t r o m e t e r s a a r e b b a c k e d b y t t h e b b e s t s s u p p o r t t t e a m i i n t t h e i i n d u s t r y .G o t t o w w w .p e r k i n e l m e r .c o m /f l u o r e s c e n c e t o l l e a r n m m o r e .The LS-55 also includes an automated Polarizer that consists of two filter wheels, each containing a horizontal and vertical polarizing element. Polarizer positions are software controlled and can be manually set or automatically controlled for polarization, anisotropy, or G-factor.INSTRUMENT COMPATIBILITYSingle-Cell Peltier AccessoryFour-Position and Single-Position Thermostatted Cell Holder Front Surface Accessory AS93plus Autosampler Accessory APPLICATION:FEATURES: APPLICATION:FEATURES:Temperature ramping studies and measuring thermal denaturation of proteins.All liquid analyses where thermostatting is required.• Single-cell,water-cooled Peltieraccessory (operation 0º to 100º C at a resolution of 0.1º)• Rapid heating and cooling • In-cuvette stirring • Control via temperature controller keypad or softwareSimple,water-cooled cuvette holders are suitable for all liquid analyses.• Controls sample temperature for accurate data collection • Cell holder accommodatesone or four 10 mm pathlength cells as well as micro-cuvettes • Requires a water bath for circulation around the cellsAnalysis of film,paper,powder,fabric,plastic film,and other flat samples.• Used for fluorescence andphosphorescence measurements • Sample can be placed in the accessory directly or held in the synthetic fused silica window powder holder• Ultra small volumes or viscoussamples can be sandwiched between the two windows• Opaque and turbid samplescan be measured in small cuvettes (under 10 mm)High throughput sampling of liquids; highly sensitive alternative to microplate reading.• Can measure up to 200 samples in a single rack• Software-controlled peristaltic pump allows collection of intensity,polarization or anisotropy measurementsSamples with weak emission (enhances the sensitivity of the fluorescence collection).• Recommended forbioluminescence andchemiluminescence measurementsRemote,non-destructive testing of fluorescent papers and fabrics orremote sampling of hazardous materials.• For taking the measurement to the sampleHigh-sensitivity liquid chromatography;alternative to a dedicated LC fluorescence detector.• Works with the monochromators of the LS-55 and LS-45 for optimal sensitivity and specificityDNA quantitation,enzyme-linked assays,protein measurements,fluorescent ELISAs,cell viability testing,and drug and steroid testing.• Measures in fluorescence,phosphorescence,bioluminescence and chemiluminescence modes • Fiberoptic light feed for permanent alignment and high performance • Uses wide range of UV and visible wavelengths • Software allows scanning of flat samples; presents data as a 3D image• Standard or far-UV fiberoptics• Easily removes and installs for switching between plate and cuvette measurements • Can create programs for single end-point and kinetics analyses • Optional plug-in software module allows the user to collect multiple spectra per well• Measures in up to 96 well platesPolarization/anisotrophy work,protein folding/unfolding and DNA melting.• Single position magnetically stirred sample holder with event marking and temperature sensor that measures from 0º to 100º CRapid data collection during biochemical processes(i.e.,intracellular ion collection).• Pairs of optical filters obtain ratio measurements every 40 milliseconds • Two pairs of filters can be fitted on each wheel• Ratio data or individual intensities can be viewed in real-timeAutomation of routine analysis and quantitation and rapid liquid sampling.• Automatically transfers sample from vessel to cuvette• Minimizes handling of quartz cuvettes • Includes software-controlled peristaltic pump and 16 µL volume flowcellTotal Emission AccessoryRemote Fiberoptic Accessory LC Flow Cell AccessoryWell-Plate Reader Accessory Biokinetics AccessoryFast Filter AccessorySipper AccessoryCall 1 800-762-4000(T o l l ff r e e U U .S .) or +1 203-925-4602for a FREE CONSULTATION with a PerkinElmer LS-55 and LS-45 specialist,and ask for an instrument and software demonstration.LS-55 and LS-45 Fluorescence Spectrometersfrom PerkinElmer –the flexibility you need,from the manufacturer you trustA perfect blend of sturdydependability and high performancePerkinElmer, Inc. is a global technology leader focused in Life and Analytical Sciences and Optoelectronics. A $1 billion market leader, the Life and Analytical Sciences division serves a large number of industries, including academic research,biotechnology, clinical screening, pharmaceutical,environmental, forensic, and the petrochemical and semiconductor segments.Our instruments, related software, and customer support programs – including our team of over 1,000 factory-trained service professionals deployed in over 125 countries – help our customers to enhance research productivity, meet strict regulatory requirements, improve time-to-market,and increase manufacturing efficiencies.The LS-55 and LS-45 FluorescenceSpectrometers are the preferred instruments for laboratories worldwide.For a complete listing of our global offices, visit /lasoffices©2006 PerkinElmer, Inc. All rights reserved. PerkinElmer and the PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. in the United States and other countries.All other trademarks not owned by PerkinElmer, Inc., or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.007693_01Printed in USACall 1 800-762-4000(T o l l ff r e e U U .S .) or +1 203-925-4602for a FREE CONSULTATION with a PerkinElmer LS-55 and LS-45 Fluorescence Spectrometer specialist .。

傅里叶变换红外光谱仪英语

傅里叶变换红外光谱仪英语

傅里叶变换红外光谱仪英语Fourier Transform Infrared SpectroscopyFourier Transform Infrared Spectroscopy (FTIR) is a powerful analytical technique used to identify and characterize a wide range of materials, including organic and inorganic compounds. This technique is based on the principle of the Fourier transform, which is a mathematical algorithm that converts a time-domain signal into a frequency-domain representation. In the case of FTIR, the time-domain signal is the interference pattern generated by the interaction of infrared radiation with a sample, and the frequency-domain representation is the infrared spectrum of the sample.The basic components of an FTIR spectrometer include a source of infrared radiation, an interferometer, a sample compartment, and a detector. The interferometer is the key component of the FTIR system, as it generates the interference pattern that is used to obtain the infrared spectrum. The interferometer typically consists of a beamsplitter, a fixed mirror, and a moving mirror. The infrared radiation from the source is split into two beams by the beamsplitter, one of which reflects off the fixed mirror and the other off the moving mirror. The two beams are then recombined and directedtowards the sample, where they interact with the sample's molecules.The interaction of the infrared radiation with the sample's molecules results in the absorption of specific wavelengths of the radiation, depending on the molecular structure and composition of the sample. The absorbed wavelengths are characteristic of the functional groups and chemical bonds present in the sample, and the intensity of the absorption is proportional to the concentration of the absorbing species. The interferogram, which is the time-domain signal generated by the interference of the two beams, is then converted into a frequency-domain spectrum using a mathematical algorithm called the Fourier transform.One of the key advantages of FTIR spectroscopy is its speed and sensitivity. Unlike dispersive infrared spectrometers, which use a monochromator to scan the infrared spectrum one wavelength at a time, FTIR spectrometers can collect the entire infrared spectrum simultaneously, resulting in faster data acquisition and higher signal-to-noise ratios. Additionally, FTIR spectrometers are generally more compact and less expensive than dispersive infrared spectrometers, making them more accessible to a wider range of users.FTIR spectroscopy has a wide range of applications in various fields, including materials science, environmental analysis, forensics, and biomedical research. In materials science, FTIR is used to characterizethe chemical composition and structure of polymers, ceramics, and other materials. In environmental analysis, FTIR is used to detect and quantify pollutants, such as volatile organic compounds (VOCs) and greenhouse gases, in air, water, and soil samples. In forensics, FTIR is used to analyze trace evidence, such as fibers, paints, and explosives, and to identify unknown substances. In biomedical research, FTIR is used to study the structure and function of proteins, lipids, and other biomolecules, as well as to diagnose and monitor various diseases.One of the key advantages of FTIR spectroscopy is its ability to provide detailed information about the chemical composition and structure of a sample. The infrared spectrum of a sample can be used to identify the presence of specific functional groups, such as carbonyl, hydroxyl, and amino groups, as well as to determine the relative abundance of these groups. Additionally, the shape and position of the absorption bands in the infrared spectrum can provide information about the molecular structure and conformation of the sample.Another advantage of FTIR spectroscopy is its versatility in sample preparation. FTIR can be used to analyze a wide range of sample types, including solids, liquids, and gases, with minimal sample preparation. Solid samples can be analyzed using techniques such as attenuated total reflectance (ATR) or diffuse reflectance, while liquid and gas samples can be analyzed using transmission or gas-phasetechniques.Despite its many advantages, FTIR spectroscopy also has some limitations. One of the main limitations is the limited penetration depth of the infrared radiation, which can make it difficult to analyze thick or opaque samples. Additionally, FTIR spectroscopy can be sensitive to environmental factors, such as temperature and humidity, which can affect the quality of the data.In conclusion, Fourier Transform Infrared Spectroscopy is a powerful analytical technique that has a wide range of applications in various fields. Its speed, sensitivity, and versatility make it an invaluable tool for researchers and analysts who need to characterize the chemical composition and structure of a wide range of materials. As the field of FTIR spectroscopy continues to evolve, it is likely that we will see even more innovative applications of this technology in the years to come.。

共振拉曼光谱在体测量类胡萝卜素含量_邵永红

共振拉曼光谱在体测量类胡萝卜素含量_邵永红

第27卷,第11期 光谱学与光谱分析Vol .27,No .11,pp2258-22612007年11月 Spectro sco py and Spectr al Analy sisNo vembe r ,2007 共振拉曼光谱在体测量类胡萝卜素含量邵永红1,何永红1,马 辉1*,南 楠2,钱龙生2,王淑霞11.清华大学深圳研究生院光学成像与传感实验室,广东深圳 5180552.中国科学院长春光学精密机械与物理研究所,吉林长春 130033摘 要 类胡萝卜素是人体内重要的抗氧化剂,能够消除体内的自由基和其它有害氧分。

研究表明类胡萝卜素含量与癌症、心血管疾病、老化等疾病发病率呈反比关系。

目前检测方法为采用血清液相色谱检测方法,不能做到在体无损伤、实时测量。

文章介绍了一种用于测量类胡萝卜素的新技术———共振拉曼光谱技术,具有在体无损伤、灵敏度高和实时检测等特点。

该技术利用强度远小于美国A NSI Z 136.1-2000标准的473nm 激光激发拇指中类胡萝卜素,产生强荧光和叠加在其上的弱共振拉曼光,通过测量拉曼散射光强度在体评估类胡萝卜素的含量。

同时,利用组织通透技术,改善了测量信噪比。

测量了不同饮食习惯志愿者的类胡萝卜素含量,说明其含量与其摄入量成正比。

该技术在临床应用和科学研究等领域具有重要意义。

关键词 拉曼光谱;激光;类胡萝卜素中图分类号:O 433.1 文献标识码:A 文章编号:1000-0593(2007)11-2258-04 收稿日期:2006-08-06,修订日期:2006-11-16 基金项目:国家自然科学基金项目(60578003)资助作者简介:邵永红,1972年生,清华大学深圳研究生院博士后 *通讯联系人 e -mail :mah ui @tsinghu a .edu .cn引 言 类胡萝卜素是一种抗氧化剂,是人体内重要的抗氧化物质[1-4]。

通常的类胡萝卜素检测方法为液相色谱法。

PR655

PR655

PR6551. IntroductionIn this document, we will discuss the PR655, a state-of-the-art instrument used for measuring color and light. The PR655 is capable of providing highly accurate and detailed color measurements, making it an essential tool for various industries such as printing, textiles, and graphic design.2. Features of the PR655The PR655 offers the following features that set it apart from other color measuring instruments:2.1 Spectral MeasurementThe PR655 utilizes a spectrometer to capture the complete spectral information of a color sample. This allows for more accurate and precise measurements compared to colorimeters that only measure a few discrete wavelength bands. The spectrometer in the PR655 covers the entire visible spectrum, enabling comprehensive color analysis.2.2 High AccuracyWith its advanced optical system and signal processing algorithms, the PR655 provides measurements with high accuracy. It has a colorimetric accuracy of less than 0.2 Delta E,ensuring minimal color difference between the measured sample and the reference.2.3 Wide Range of ApplicationsThe PR655 can be used for a wide range of applications, such as color control in printing and packaging, quality assurance in textile manufacturing, color matching in graphic design, and analysis of LED and display technologies. Its versatility makes it a valuable tool for professionals in various industries.2.4 User-Friendly InterfaceThe PR655 features an intuitive user interface that allows for easy operation. It has a color touchscreen display where users can view measurement results, configure settings, and access various measurement modes. The instrument also offers USB connectivity for data transfer and software updates.3. Measurement ModesThe PR655 offers different measurement modes to cater to various requirements:3.1 Reflectance ModeIn reflectance mode, the PR655 measures the color of an object by analyzing the light reflected from its surface. This mode is commonly used for color analysis of paints, textiles, plastics, and other opaque materials.3.2 Transmittance ModeTransmittance mode is used to measure the color of transparent or translucent materials. The PR655 measures the light transmitted through the sample and provides accurate color information. This mode is useful for analyzing the colorof glass, films, liquids, and other similar materials.3.3 Single Measurement ModeIn single measurement mode, the PR655 performs a one-time measurement of the color sample and displays the results immediately. This mode is suitable for quick color checks and simple color matching tasks.3.4 Continuous Measurement ModeContinuous measurement mode allows the PR655 to take continuous measurements at a predefined frequency. This mode is useful for monitoring color consistency in production lines or when capturing color changes over time.4. Data Analysis and ReportingThe PR655 comes with software that enables users to analyze and report measurement data. The software allows for the creation of color profiles, comparison of measurement data, and generation of color difference reports. It also provides advanced analysis tools for in-depth color analysis and visualization.5. ConclusionThe PR655 is a powerful and versatile instrument for color and light measurement. With its accurate measurements, wide range of applications, and user-friendly interface, it is an essential tool for professionals in industries that rely on precise color control. Whether you are in the printing, textile, or graphic design industry, the PR655 will greatly enhance your color analysis capabilities and improve your overall workflow.Note: The content of this document is for informational purposes only and does not constitute any form of endorsement or recommendation.。

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a rXiv:as tr o-ph/311566v125Nov23The GraF instrument for imaging spectroscopy with the adaptive optics †A.Chalabaev,E.le Coarer,P.Rabou,Y.Magnard and P.Petmezakis Laboratoire d’Astrophysique,Observatoire de Grenoble,UMR 5571,CNRS and Universit´e Joseph Fourier,B.P.53X,F-38041Grenoble,France D.Le Mignant W.M.Keck Observatory,65-1120Mamalahoa Highway,Kamuela HI96743,U.S.A.Abstract.The GraF instrument using a Fabry-Perot interferometer cross-dispersed with a grating was one of the first integral-field and long-slit spectrographs built for and used with an adaptive optics system.We describe its concept,design,optimal observational procedures and the measured performances.The instrument was used in 1997-2001at the ESO 3.6m telescope equipped with ADONIS adaptive optics and SHARPII+camera.The operating spectral range was 1.2-2.5µm .We used the spectral resolution from 500to 10000combined with the angular resolution of 0.1′′-0.2′′.The quality of GraF data is illustrated by the integral field spectroscopy of the complex 0.9′′×0.9′′central region of ηCar in the 1.7µm spectral range at the limit of spectral and angular resolutions.Keywords:instrumentation:spectrographs,instrumentation:adaptive optics,tech-nics:spectroscopic,infrared:stars,stars:individual:ηCar 1.Introduction High angular resolution observations at the diffraction limit of the ground based large telescopes were pioneered using the speckle anal-ysis of images (Labeyrie,1970),however their sensitivity was severely limited by the necessity to keep exposures short in order to “freeze”the turbulence induced patterns.The adaptive optics overcame this obstacle allowing long exposures (COME-ON and ADONIS at the ESO 3.6m telescope,Beuzit et al.,1997,PUEO at the CFHT,Rigaut et al.,1998,NAOS at the ESO VLT,Lagrange et al.,2003,and others).The limiting magnitude on the telescopes equipped with the adaptive optics (hereafter AO)is now defined by the detector and optics efficiency as for any observations,while the angular resolution is close,at least in the IR,to the telescope diffraction limit,about 0.1′′for a 4m aperture at λ=2µm .The achievements of the AO were firstly exploited for imaging (see reviews by Close,2000,Lai,2000,Menard et al.,2000).The next step2Chalabaev et al.was to use the adaptive optics for spectroscopy.Indeed,the combination of high angular and high spectral resolutions is a key issue for a number of observational programmes such as physics and evolution of multiple stellar systems,morphology and dynamics of circumstellar gas and jets in young and evolved stars,etc.Driven by this ideas,the GraF project was started in1995with the aim to build an imaging spectrograph optimized for the use with the ADONIS at the ESO3.6m telescope.The instrument is based on the integralfield spectroscopic properties of the Fabry-Perot interferometer (hereafter FPI)used in cross-dispersion with a grating(le Coarer et al., 1992,1993).It was tested at the telescope in1997and successfully used for a number of astronomical programmes in1998-2001(for preliminary results see Chalabaev et al.,1999a,1999b,1999c,Trouboul et al.,1999).In the present article we describe in details the optical concept and the instrument built tofit the constraints imposed by ADONIS(Beuzit et al.,1997)and the SHARPII+camera(Hofmann et al.,1995).We give the account of the observational procedures emerged from our experience as optimal,present the measured performances,and illus-trate them by a sample of reduced data obtained at the limit of the instrument possibilities in terms of the angular and spectral resolutions.The discussion will be restricted to the applications of a high spec-tral resolution(R≃5000-10000)in a moderate passband(∆λ≤50) combined with a high angular resolution(≃0.1′′-0.2′′)in a smallfield of view(F OV≤15′′).The operating spectral range of the instrument was1.2−2.5µm.We hope that this one of thefirst experiences of the imaging spec-troscopy with the adaptive optics(see also Bacon et al.,1995,Lavalley et al.,1997)will be useful for designers and users of future spectro-imaging instruments combining the high angular and high spectral resolutions.2.Image restoration aspectsLet us in what follows to make the emphasis of the discussion on the scarcely resolved objects,i.e.having the spatial1spectrum in the Fourier domain comparable in the extent to that of the AO corrected telescope modulation transfer function(MTF).There are two reasons for such a choice.Firstly,as witnessed by the reviewers(cf.Close,2000,Lai,2000,Menard et al.,2000),the AO provides a considerable scientific contribution mainly in the case whereGraF instrument for imaging spectroscopy3 the object under study,having remained point-like at lesser angular resolutions,becomes scarcely resolved,revealing new spatial features.The second reason is dictated simply by the fact that the scarcely resolved objects are the most difficult to measure,so that the overall performance of a new instrument at the limit of resolution is best evaluated on such objects.We can already note that the importance of the scarcely resolved ob-jects shapes the specifications on the AO assisted spectrograph.Indeed, to recover the information up to the highest possible spatial frequency implies the image restoration,and this aspect has necessarily to be taken into account in the design of the spectrograph.2.1.GeneralWe shall consider theflux density distribution S(x,y,λ)which is non-zero at least at2points of the two-dimensional(2D)skyfield{x,y}. Here,x and y are the spatial coordinates,andλis the wavelength.As it was already said in Sect.1,the discussion will be restricted to small fields,≤15′′.The image restoration problem of imaging spectroscopy in the gen-eral case consists infinding the best estimateˆS(x,y,λ)of the objectflux distribution density S(x,y,λ),satisfying the tri-dimensional integral equation of convolution:F(x,y,λ)= dξdζdw·S(ξ,ζ,w)·G(ξ−x,ζ−y,w−λ)(1)where F is the measuredflux density distribution and G is the instru-mental impulse response.The solution of the integral equation of convolution is known to be unstable(Turchin et al.,1971;see also Lucy,1994a).The calibration errors of G are strongly amplified in thefinal result of image restoration, in particular at high frequencies,so that the solutionˆS has to be regularized,i.e.searched in a space of appropriate smooth functions (see for details Tikhonov and Arsenin,1977,Titterington,1985,Lucy, 1994b).The image restoration can be carried out using either one of the many proposed deconvolution algorithms(e.g.Cornwell,1992,Magain et al.,1998,Lucy and Walsh,2003,and references therein),or by mod-elingˆS from a priori defined physical considerations and then searching for the bestfit of the convolution productˆS⊗G to F within the physical model.It is clear that whatever the method adopted for the image restora-tion,the accurate calibration of G is of high importance.Let us analyze4Chalabaev et al.the sources of the calibrations errors in the case of imaging spectroscopy in order to get guidelines of the instrumental concept.2.2.Calibration errorsFirstly,we can simplify the Eq.(1)by noting that for the considered here angularfields,spectral passbands and resolutions(see Sect.1)the 3D impulse response G can be written as the product of the spatial point-spread function(psf)P(x,y|ξ,ζ;λ)and the instrumental spectral profile L(x,y;λ|w)(see e.g.Perina,1971,Goodman,1968,Mariotti, 1988).Then we get:F(x,y,λ)= dw·L(x,y,λ−w) dξdζ·S(ξ,ζ,w)·P(ξ−x,ζ−y,λ)(2)We will further assume that the relevant scientific information can be extracted without deconvolution onλ.In other words,we will limit the discussion to the frequently encountered case where the instrumental spectral profile L is much narrower than the studied spectral lines.We can then write:L(x,y,λ−w)≃L(x,y,λ)·δ(λ−w)(3) where L(x,y,λ)is the spectral transmission,a simple multiplicative factor,andδ(λ−w)is the Dirac impulse function.The convolution concerns now only the spatial dimensions,so that the Eq.(2)is further simplified as follows:F(x,y,λ)=L(x,y,λ) dξdζ·S(ξ,ζ,λ)·P(ξ−x,ζ−y,λ)(4)The important consequence is that in the absence of the deconvolution onλthe contribution of the calibration errors on L to the uncertainty of thefinal estimateˆS is considerably reduced.Furthermore,we note that usually the variations of L during obser-vations are due to mechanicalflexure at the telescope.They are slow,with the time scale of tens of minutes,and can be monitored with agood accuracy.Similarly,the dependence of L on x and y is stable andcan be calibrated accurately.In contrast to the relative stability of L,the psf P undergoes signif-icant temporal variations on the time scale of minutes or even shorterdue to atmospheric turbulence.Although the AO improves dramati-cally the situation,the residual wavefront variations can still be sig-nificant.Their amplitude depends on the operating wavelength,theamplitude and the time scale of the atmospheric turbulence,the per-formances of the AO system and the telescope aperture size.With theGraF instrument for imaging spectroscopy5 ESO3.6m telescope and the ADONIS,the variations of P expressed in terms of the Strehl ratio were found to change from10to35in the K band for the seeing value of≈1.5′′within the time interval of10s (Le Mignant et al.,1999).The variability is a fortiori stronger at the shorter wavelengths of J and H bands.As to the wavelength dependence of P,it is negligible within the considered here spectral range of a single instrument setting,∆λ/λ≤50.We can conclude that the main source of errors in thefinal result of image restoration,theflux distribution estimateˆS,comes from the calibration of the spatial psf P due to its rapid variability.The way it is calibrated needs thus special attention.For instance,the calibration errors can be substantially reduced if P is measured simultaneously with the measurement of F,e.g.on a suitable source in the samefield. If P can be measured only on a source offthefield,it must be done as close in time as possible.The instrumental concept has to take into account these observational aspects.It also appears important that the two-dimensional{x,y}-structure of thefield and of the psf P is recorded with no scanning2on{x,y},in order to keep the error on P homogeneous over x and y,thus avoiding a random scrambling of spatial features,which can be unrecoverable.As to the spectral response L,the contribution of its calibration errors in thefinal uncertainty onˆS is considerably lesser than that of P.Indeed,the instrumental spectral profile L is relatively stable and can be calibrated much more accurately than P.Furthermore,if no deconvolution is justified onλ,i.e.if the spectral resolutions high enough,then the error on L propagates into the error onˆS without amplification.2.3.Implications for the AO assisted spectroscopyThe above given analysis provides the guidelines of the instrumental concept for the AO assisted imaging spectroscopy which can be briefly summarized as follows:(i)the calibration of the psf P has to be simul-taneous,or as close in time as possible,to the measurement of theflux distribution F;(ii)the scanning over the spatial x-and y-axes must be avoided;(iii)if scanning is unavoidable due to the volume of data to be recorded,the instrument concept allowingλ-scanning is preferable.Obviously,the ideal spectro-imaging instrument would record the entire cube of data F(x,y,λ)in one single exposure with no scanning,6Chalabaev et al.satisfying the criteria of both the accurate image restoration and the saving the telescope time.The work is in progress on the“3D detectors”able to record both the position x,y and the energyλof the photons,cf.the superconducting tunnel junctions(Perryman et al.,1994,Rando et al.,2000)or the dye-doped polymers(Keller et al.,1995).However,their sensitivity is still less convincing than that of the modern2D-detectors.The best available today solutions are offered by the optical set-ups known as the integralfield spectrographs(IFS,Courtes,1982,Bacon et al.,1995,Weitzel et al.,1994,Le Fevre et al.,1998,Eisenhauer et al., 2000).They use the2D detectors to record F(x,y,λ)with no scanning, provided the detector size is large enough to record the3D data cube at once.3.Particular case of a“linear image”Let us also consider the simplest case of the imaging spectroscopy when the objectflux density S(x,y,λ)is non-zero along a straight line,so that S is a function of only two variables,S(x,λ).In this“linear”case,frequently encountered in the astrophysical practice(e.g.binary stellar systems),the common grating spectro-graphs recording F(x,λ)at a single setting offer a suitable solution, insuring the calibration of psf P homogeneous over the studiedfield {x}.Furthermore,in the case of a circumstellar nebular object,when the extended feature is a gas emitting only in spectral lines,the emission in the continuum corresponds to the point-like star and provides the calibration of P(x)simultaneous to the measurement of F.4.The minimum3D data cube volumeAt high angular and high spectral resolutions,the volume of data to be recorded in one observation of imaging spectroscopy can be large. Let us to estimate what is its minimum under typical astrophysical specifications.Empirically,the angularfield of≃3′′×3′′would be adequate to study most types of objects of interest of the stellar physics.The pixel size of0.05′′isfixed by the angular resolution,which is≈0.1′′at a4m telescope atλ=2µm.Thus,the record of the spatialflux distribution at a given wavelength Fλ(x,y)consists of60×60=3600data values. Along theλ-axis,the minimum of about100points is suitable in order to record the profile of a spectral line and the adjacent continuum.GraF instrument for imaging spectroscopy7Figure1.The optical concept of the the imaging spectrograph using a Fabry-Perot interferometer in cross-dispersion with a grating,replaced here by a grism for the convenience of the drawing.Thus,the entire cube data volume is at least60×60×100≃6002 values.This largely exceeds the2562size of the detector available with ADONIS/SHARPII+.In our case the IFS approach is clearly unafford-able,the scanning is imposed.Then,in agreement with the conclusions of error analysis in imaging spectroscopy given in Sect.2.3,we adopted the concept of theλ-scanning imaging spectrograph described below.5.The GraF concept5.1.Optical scheme.Data cube structureThe concept uses a Fabry-Perot interferometer in cross-dispersion with a grating(hence the acronym we gave for this concept,GraF=Gra(ting) and F(abry-Perot).The set-up(see Fig.1)wasfirst described by Fabry (1905),and used for spectroscopy by Chabal and Pelletier(1965)and Kulagin(1980).The IFS property of the set-up was noticed by le Coarer (1992)and demonstrated by le Coarer et al.(1992,1993).Baldry and Bland-Hawthorn(2000)went further,describing a tunable echelle im-ager,where a FPI is cross-dispersed with a grism and with an echelle grating.In a single frame,GraF records the quasi-monochromatic images of thefield Fλ(x,y)corresponding to several values ofλ,which is the distinctive property of an IFS instrument.The spectrum is sampled by a comb of the FPI transmission peaks(interference orders)separated by the interorder wavelength spacing∆λf(Sect.6).The whole set ofλvalues is recovered by scanning.The structure of the spectro-imaging data cube is illustrated in Fig.2.The FPI acts as a“multi-passband”filter.The light of different8Chalabaev et al.FPI orders is sorted by the grating according their wavelength.The resulting series of quasi-monochromatic images of the skyfield is formed in the output focal plane.Note that the width3of the entrancefield must be limited by a focal aperture to avoid superposition of order images.Anticipating a detailed discussion(Sect.6),let us give thefigures of a typical cube volume recorded with the actually built instrument. At each step of scanning,the detector frame of2562pixels records8 narrow-band images corresponding to the dispersed sequence of the FPI orders as selected by the grating angle value.Each FPI“order image”covers the same1.5′′×12.4′′region of the entrance skyfield sampled with the pixels of0.05′′,which makes≃30×250=7440spatial pixels.This is close to the estimated minimum required by the stellar obser-vations,although,the width of the FOV≃1.5′′is a factor of2less than the desirable≃3′′value.For the observations of the elongated objects, this shortage is partially compensated by the considerable height of the FOV of≃12′′,which can be suitably aligned.The spectral band covered by the detector is about40nm atλ=2.2µm,corresponding to8FPI orders.It is scanned in48FPI channel frames4,although in special cases one can limit the scan to a narrower range of interest.The number of spectral samples in a full FPI scan is48×8=384.The spectral passband of an image isδλ≃0.3nm atλ=2µm,so that the corresponding spectral resolving power is R=λ/δλ≃7000.The total volume of the cube is48×2562=3.15·106 pixels,or≃17702pixels.5.2.Advantages and limitations of the conceptThe GraFλ-scanning IFS appears as a suitable solution when scan-ning is imposed by the modest detector size.It allows a simultaneous record of several monochromatic images of a reasonably largefield thus keeping possible an accurate image restoration.Scanning is done by the comb of spaced FPI transmission peaks rather than by a contiguous set of the wavelength values like in a grating based instrument.The peaks spacing makes more certain to have in each frame at least one“order”image placed at the wave-lengths of the continuum emission,thus providing a reference signalGraF instrument for imaging spectroscopy9F P I o r d e r s = “G r a F w i n d o w s ”G r a t i n g d i s p e r s i o n λ - a x i sFPI channel sequence∆w fλ nλ m Figure 2.The structure of the GraF IFS cube.Left :The “Southern Crab”planetary nebula as it appears in the white light (simulated).The field of view is limited by a rectangular entrance aperture.Right :The images of the nebula in the light of a atomic spectral line (simulated image)as they appear in the focal plane of the GraF instrument.Each frame consists of several monochromatic “windows”corresponding to the FPI orders.Due to the differential motion of the nebulae,the aspect of the nebula is changing from one “window”to another.for photometric monitoring and,in the nebular cases,the calibration of the P simultaneous to the measurement of F .Another convenient point of the concept is the simplicity of trans-forming a grating spectrograph into a GraF instrument by adding solely a FPI and an adjustable slit (see also comments by Baldry and Bland-Hawthorn,2000).Vice versa ,the instrument is easily switched to a grating spectrograph for observations of “linear”objects.However,the GraF concept is intrinsically scanning,so that if scan-ning is unnecessary,the GraF is slower than other mentioned above IFS concepts.Further,the width of the FOV has an upper limit.Expressed in the elements of the angular resolution δφ,the FOV width cannot exceed the FPI finesse value F (see Sect. 6.2).For the maximum F of ≃40still suitable for the high-throughput imaging (Bland-Hawthorn,1995),the maximum FOV width of a GraF is ≃4′′for δφ≃0.1′′.10Chalabaev et al.6.Formulae for GraF optical parameters6.1.FPI formulaeLet us remind the basic terms describing the FPI properties.The FPI spectral transmission is a comb of peaks,called“orders”,occurring at λm defined by the condition of the interference:mλm=2ne cos i(5) where the integer m is the order of the interference,n the refractive index,e the gap between the FPI plates,and i the angle of incidence.The value ofλm varies over the FOV according to the value of i. However,as it can be estimated from Eq.5,this variation is negligible:δλ/λ≃5·10−9for the consideredfields of view≤15′′.In what follows, it will be assumed that i=90◦,so that the gap e defines completely the set ofλm.The distance between two neighbor orders is called the interorder spectral spacing.Expressed in the wavelength units,∆λf,it can be written from Eq.5as follows:∆λf=λmλm+12ne(6)It is often more convenient to use the interorder spacing expressed in the wavenumber units∆σf,which has no spectral dependence:∆σf=1δλ(8) The instrumental profile L is the Airy function:L=11+(2σ/δσ)2(10)Using Eqs.(6)and(8),the spectral resolving power R=λ/δλcan be formally written as follows:R=2ne F2δλ.The trouble with FPI is that the Fourier transform of L is neverzero5and has no f cut.Thus,formally FPI escapes the Shannon theorem.In practice,the effective number of reflexions on the FPI plates, hence the effectivefinesse F and the effective OP D max,is limited by the noise,so that f cut can be defined on this basis.For the same instrument, it will vary depending on the S/N ratio.The uncertainty on f cut,and hence on h s,explains the choice of a conservatively small value of h=δλ/2.7.6.2.GraF formulae.The limits and optimum for the FOVwidthIn the absence of the grating,the output image would be a superposi-tion of all images transmitted in the passbands of the FPI orders.The grating deviates each order light into a specific angle according to its wavelengthλm,and thus spatially sorts the order images(see Fig.2).In the output focal plane,the shift between the order images∆w f is equal to the interorder spectral spacing∆λf scaled by the reciprocaldispersion rdisp(expressed in nm/pix)as follows:∆w f[pix]=∆λf(14)rdispAlbeit shifted according their wavelength,the order images superim-pose.The confusion isfinally avoided by limiting the width of the entrance FOV to the value∆w f.The latter,expressed in arcsec,is the confusion free width of the FOV,or simply the free width(see Fig.2).The Eq.(13)seems to indicate that the free width∆w f can be increased arbitrarily by increasing the grating spectral dispersion.How-ever,the extension of the output psf along theλ-axis,measured as the FWHMδφgraf,disp,also increases with the dispersion due to collinearity ofλ-and y axes.We can write:P graf,disp= dw·(M F P(w)·P ao(w))L gr(w−λ)(15)where P graf,disp is the psf profile along theλ-axis at the GraF output, P ao(λ)is profile of the monochromatic psf at the output of the adaptive optics,M F P I is the spectral transmission of the FPI,and L gr(w−λ) is the instrumental spectral profile of the grating.For the corresponding FWHM’s,we get:δφgraf,disp[pix]≃ rdisp 2(16) which gives the explicit relation ofδφgraf,disp and rdisp.To get the insight into the tradeoffguiding the choice of the spectral dispersion value rdisp,let us introduce the dimensionless free width of FOV,∆w el,expressed in the number of the spatial elements of resolutionδφgraf,disp as follows:∆w f rdisp∆w el=δφ2ao+ δλ=F(18)δλ1.00.80.60.40.20.0 86420Spectral sampling [pix/δλ]N e l N e l ,m a x pix/δφ = 232.54 ∆w e l /∆w e l ,m a x Figure 3.The ratio of the free width expressed in number of resolution elements ∆w el to its asymptotic maximum ∆w el,max =F in function of the spectral (along the grating dispersion)and spatial sampling ratios.The finesse F sets the asymptotic upper limit to the number of spatial elements that one can get along the width of the GraF FOV.This is illustrated in Fig.3,which displays the ratio of ∆w el to the possible maximum ∆w el,max in function of the spectral sampling ratio along the grating dispersion ρλ.The latter is defined as the number of pixels for one element of resolution,ρλ=δλ/rdsip .The curves are computed for several values of the spatial sampling ratio,ρs =δφao /H .One can see that ∆w el /∆w el,max increases rapidly up to ρλ≃2.5and saturates afterwards.The range of rdisp and ∆w f corresponding to ρλ≃2.5can be considered as optimum.Higher dispersions will give only a slight increase of ∆w el /∆w el,max ,while the number of order windows covered by the detector,M wind ,will decrease linearly.The numerical values of the free width ∆w f chosen for the instru-ment are close to the optimum.They and are given in Tab.III in arcsec and pixels .The corresponding value of ∆w el ,for instance at λ=2.2µm,is ∆w el ≈7.The other parameters are the platescale H =50mas/pix (hereafter mas states for milli −arcseconds ),rdisp of the 300mm −1grating,the free width ∆w f =1.55′′and the measured psf extension δφgraf,disp =0.22′′(see Sect.10.1).The maximum possible width at this wavelength is ∆w el,max =F ≈16.8,as calculated from ∆λf =4.75nm and the measured spectral res-olution δλ=0.3nm of the FPI.The measured ratio ∆w el /∆w el,max ≈0.45is close to the theoretical value of 0.55derived from Fig.3for the used spatial and spectral samplings ratios respectively ρs =2.6and ρλ=1.9.Figure4.The GraF optical design.7.Implementation at the telescope7.1.HardwareThe implemented spectrograph has been designed to meet the ADONIS imposed mechanical constraints,dictating a compact(1.5m×0.5m) and light(30kg)instrument(see Fig.4).The operating wavelength range from1.2µm to2.5µm corresponds to that of the SHARPII+ camera.The spectrograph was installed at the ADONIS visitor equipment bench(Beuzit et al.,1997),located between the adaptive optics output and the SHARPII+camera.Theflat mirror M in intercepts the ADO-NIS f/45output beam and sends it into the spectrograph.The beam passesfirstly through afield rotator made of a prism and aflat mirror. Thefield rotator of GraF allows to vary the slit orientation on the sky,which otherwise would have been remainedfixed due to ADONIS constraints.The FOV is selected by the rectangular aperture located in the ADONIS focal plane.Its width,can be adjusted continuously and with a good precision from0.1′′to15′′,and its height isfixed to about 30′′.After the aperture,the beam is collimated,passes through the Fabry-Perot interferometer,is dispersed by the grating,reconfigured by the spectrograph camera back to the f/45beam and sent to the SHARPII+camera.The instrument can also be used in a direct imaging mode,either by setting the grating at the zero order position,or replacing it by aflat mirror mounted at the back of the grating(not shown in Fig.4). In this configuration the FPI is used in the classical scanning mode.The twoflat mirrors M in and M out,located on the axis of the ADONIS-SHARPII+camera,are mounted on a dedicated common support,so that they can be removed and installed within minutes,al-lowing a quick change from the GraF IFS mode to the regular ADONIS imaging observations.The motors controlling the focal aperture,thefield rotator,the grating position,the FPI in-and out-of the beam movements,are operated through the GraF dedicated version of the ADOCAM real-time operations software written by combe.The GraF operation at the telescope thus inherited conveniently from the user-friendly inter-face of the wide-band imaging ADONIS observations,and in particular the possibility to launch the command sequences in the batch mode.7.2.Optical qualityThe necessity tofit the instrument into a reduced room implied adding 5moreflat mirrors in the optical design,further,the necessity of thefield rotator implied adding3mirrors more.In total,it makes11 reflecting surfaces.The loss in transmission is limited by the high effi-ciency golden coatings.However,this extra number of optical surfaces certainly decreased the spectrograph image quality.Estimating that a mirror is manufactured withλ/10precision,andλ=0.6µm,the accumulated rms wavefront error should be about200nm,orλ/5at the shortest operating wavelength of1.2µm,which can be considered as still acceptable.More importantly,these static aberrations,at least at low and mod-erate spatial frequencies,are corrected by thefine tuning of the AO,so that the degradation of thefinal image quality is negligible as witnessed by the stellar images given in Fig.7.The AO tuning is done at the beginning of each night.7.3.Observing modesThe Table I summarize the observing modes of the GraF instrument.It shows an apparently complex instrument,while in practice the change from one mode to another is done in a few dozen of seconds or in a few minutes at longest,and can be programmed beforehand using the ADONIS/ADOCAM control software scripts.The availability of the modes of direct imaging and of grating spectroscopy(hereafter GS) was very valuable during the tests,providing independent and comple-mentary measurements for the IFS mode.Furthermore,the GS mode。

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