紫外和干旱胁迫对转基因水稻生理和抗氧化酶的影响

紫外和干旱胁迫对转基因水稻生理和抗氧化酶的影响
刘文;巩健;张承仁;赵凤云
【期刊名称】《种子》
【年(卷),期】2011(30)9
【摘要】以携带谷胱甘肽转移酶(GST)和过氧化氢酶(CAT1)的转基因水稻和非转基因水稻(Oryza sativaL.中花11)的地上部分为材料,比较分析了二者在不同强度紫外胁迫及紫外和干旱复合胁迫下生理和抗氧化酶活性的变化.结果表明,单一紫外和紫外+干旱复合胁迫条件下,转基因水稻的株高、相对含水量、叶绿素含量、光合效率及GST、CAT、SOD和APX的活性都高于非转基因水稻；而转基因水稻MDA 的积累量则低于非转基因水稻；说明转基因水稻对紫外和干旱胁迫的抵抗能力有所提高.此外,本实验条件下,紫外+干旱复合胁迫比单一紫外胁迫(尤其在强辐射组)对植株的伤害轻,说明干旱对紫外胁迫有一定的缓解作用,这种缓解作用对转基因水稻更加明显.%A comparative study on changes of physiology and antioxidant enzyme in transgenic rice carrying glutathione-S-transferase (GST,EC.2. 5. 1.
18) and catalasel (CAT 1,EC. 1. 11. 1.6) and non-transgenic rice ( Oryza sativa L. Zhonghua No. 11) exposed to UV-B radiation stress or combined UV-B radiation and 10% PEG drought stress was conducted. The results showed that when subjected to UV-B radiation and combined UV-B radiation and drought, the shoot length, relative water content, total chlorophyll content and photosynthetic rate were higher in the transgenetic rice ( T) than that of non-transgenetic rice ( N ). The content of MDA were lower in T than that of N, and the activity of GST, CAT, SOD,
APX in the shoots of T was higher than that of N. The results indicated that the expression of GST and CAT in the transgenic rice resulted in increased tolerance to UV and drought stress. Moreover,under this experiment condition,less damage was observed in the combined UV-B radiation and drought (especially under strong radiation) treated rice than that of single UV-B radiation treated one. It implied that drought stress relieived ultraviolet stress to certain degree, especially in transgenetic rice.
【总页数】5页(P14-17,21)
【作者】刘文;巩健;张承仁;赵凤云
【作者单位】山东理工大学生命科学学院,山东淄博255049;山东省淄博职业学院制药与生物工程系,山东淄博255314;山东理工大学生命科学学院,山东淄博255049;山东理工大学生命科学学院,山东淄博255049
【正文语种】中文
【中图分类】S511
【相关文献】
1.模拟干旱胁迫对转 C4双基因水稻幼苗光合功能及部分抗氧化酶活性的影响 [J], 张小娟;宋涛;甄晓辉;苑中原;马静;张入峰;高志萍;吕川根;陈国祥
2.盐碱胁迫对转Lc-CDPK基因水稻抗氧化酶活性及基因表达的影响 [J], 陈展宇; 费小钰; 孙帆; 崔喜艳
3.外源5-氨基乙酰丙酸对干旱胁迫下玉米幼苗生理特性及抗氧化酶基因表达的影响 [J], 王鹏;王铁兵;王瑞;闫蕊;史珍;常巧玲;王芳
4.干旱胁迫对转PtWOX11/12 a基因河北杨生长及生理特性的影响 [J], 常宇璇;闫盟;张骐;李佳琪;燕兴;淑芳;王亚萍;白玉娥
5.重度干旱胁迫下草甘膦对转基因大豆生理特性及光系统Ⅱ蛋白表达的影响 [J], 常丽娟;刘文娟;张富丽
因版权原因，仅展示原文概要，查看原文内容请购买。