巴戟天对骨质疏松破骨细胞表面Ⅰ型跨膜受体蛋白的影响

巴戟天对骨质疏松破骨细胞表面Ⅰ型跨膜受体蛋白的影响王筠;苗德胜;吕刚;莫新元
【期刊名称】《中国组织工程研究》
【年(卷),期】2016(020)024
【摘要】BACKGROUND:Morinda has been reported to promote the proliferation, the secretion of alkaline phosphatase and osteocalcin, and mRNA expression of transforming growth factor of osteoblasts. However, little information is available addressing the effects of Morinda on receptor activator of nuclear factor-κB expression in osteoclasts in rats with osteoporosis. OBJECTIVE:To study the effects of Morinda on receptor activator of nuclear factor-κB expression in osteoclastsofosteoporosis rats. METHODS:Thirty Sprague-Dawley rats were equaly and randomly divided into Morinda and 17β-estradiol groups. Rat models of osteoporosis were established by bilateral ovariectomy, and then 5 mL of Morinda
decocta(1.0mmol/L)and 17β-estradiol (1×10-6mmol/L) were administered intragastricaly to rats in Morinda and 17β-estradiol groups for 3 consecutive months, respectively. Primary osteoclasts were isolated from rats in both groups, andthen cultured for 3, 6 and 9 days folowed by TRAP staining andcelcounting. Bone mineral density of the proximal and distal femur, urine and serum levels of Ca2+and progesterone, and receptor activator of nuclear factor-κB expression in osteoclasts ofrats in both groups were determined. RESULTS AND CONCLUSION:Osteoclast fusion
was reduced in Morinda group. In contrast, number of osteoclastswas increased andcels becamemore maturein the17β-estradiol group. Bone mineral density of the proximal and distal femur bilateraly, urine and serum levels of Ca2+and progesterone were significantly increased, while receptor activator of nuclear factor-κB expression was significantly decreased in osteoclasts in Morinda group compared with 17β-estradiol group (P< 0.05). These results indicate that Morinda reduces receptor activator of nuclear factor-κB expression in osteoclasts in osteoporosis rats, thereby inhibiting the development and progression of osteoporosis.%背景：研究显示巴戟天能够直接刺激体外成骨细胞增加，促进成骨细胞分泌碱性磷酸酶和骨钙素，促进成骨细胞表达转化生长因子β1mRNA。

但是，对于巴戟天对骨质疏松大鼠破骨细胞RANK的影响机制尚缺乏报道。

目的：分析巴戟天对骨质疏松大
鼠破骨细胞表面的Ⅰ型跨膜受体蛋白的影响机制。

方法：采用随机对照方法将30
只SD大鼠分为巴戟天组和17β-雌二醇组，每组15只。

采用腹腔注入盐酸氯胺酮麻醉，切除双侧卵巢，建立大鼠骨质疏松动物模型，造模后大鼠正常饮食。

17β-
雌二醇组灌胃给予5 mL浓度10-6 mmol/L的17β-雌二醇；巴戟天组灌胃给予5 mL浓度为1.0 mmol/L巴戟天煎剂，连续给药3个月后，体外分离培养大鼠原代破骨细胞，培养第3，6，9天进行破骨细胞TRAP染色并计数；观察两组大鼠股
骨近远端骨密度值；检测两组大鼠尿Ca2+水平、血清Ca2+以及血清P水平；检测两组大鼠RANK表达情况。

结果与结论：①破骨细胞培养第3，6，9天后，巴戟天组大鼠破骨细胞融合相对较少，并且破骨细胞融合程度减少，细胞体积相对较大，酶活性部位呈现红色。

17β-雌二醇组大鼠细胞数相对较多，细胞也相对比较
成熟；②巴戟天组大鼠左右侧股骨近、远端骨密度显著大于17β-雌二醇组
(P<0.05)；③巴戟天组尿Ca2+水平、血清Ca2+以及血清P水平，显著高于
17β-雌二醇组(P<0.05)；④巴戟天组大鼠RANKA值、RANK mRNA表达显著低于17β-雌二醇组(P<0.05)。

⑤结果提示，巴戟天能降低骨质疏松大鼠骨细胞RANK表达，抑制骨质疏松的发生、发展，从而发挥对骨质疏松的保护作用。

【总页数】7页(P3516-3522)
【作者】王筠;苗德胜;吕刚;莫新元
【作者单位】新疆医科大学附属中医医院骨一科，新疆维吾尔自治区乌鲁木齐市830000;新疆医科大学附属中医医院骨一科，新疆维吾尔自治区乌鲁木齐市830000;新疆医科大学附属中医医院骨一科，新疆维吾尔自治区乌鲁木齐市830000;新疆医科大学附属第一医院，新疆维吾尔自治区乌鲁木齐市 830054【正文语种】中文
【中图分类】R318
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