壮药柿叶槲皮素和山柰素含量测定方法的建立

壮药柿叶槲皮素和山柰素含量测定方法的建立
覃冬杰;梁永红;黄瑞松;刘华钢;张鹏
【期刊名称】《广西医学》
【年(卷),期】2012(034)009
【摘要】Objective To investigate the method for content determination of quercetin and kaempferol in persimmon leaves. Methods To research the chromatographic conditions of high performance liquid chromatography ( HPLC) , then the orthogonal design was applied to study the samples' preparation conditions. Results Chromatographic condition as follows : Hypersil ODS2 C18(4.6 mm ×250 mm,5 μm) , the mobile phase consisted
of methanol -0.4% phosphoric acid(50 '?50) with the flow of 1.0
ml/min,and the detection wavelength was 360 nm. The samples' preparation conditions as follows; The fragmentation degree was 60 mesh, extraction solvent was methanol -25% hydrochloride (4:1) ,solvent inventory amounted to 10 ml,reflux time was 1 hour. Conclusion The HPLC method we established is accurate and reliable , which can be used for determination of quercetin and kaempferol in Zhuang medicine of persimmon leaves.%目的建立柿叶槲皮素和山柰素含量测定方法.方法对HPLC 方法中的色谱条件进行摸索,并采用正交设计法考察供试品溶液的制备条件.结果
色谱条件为:Hypersil ODS2 C18柱(4.6 mm×250 mm,5 μm),流动相为甲醇-0.4%磷酸溶液(50:50),流速1.0 ml/min,检测波长360 nm.样品供试液制备条件为:药材破碎度过60目筛,提取溶剂为甲醇-25%盐酸(4:1),溶剂加入量10 ml,回流时间1 h.
结论建立的HPLC含量测定方法准确可靠,可作为壮药材柿叶中槲皮素和山柰素总量的测定方法.
【总页数】4页(P1133-1136)
【作者】覃冬杰;梁永红;黄瑞松;刘华钢;张鹏
【作者单位】广西医科大学药学院,南宁市,530021;广西医科大学药学院,南宁市,530021;广西民族医药研究院,南宁市,530001;广西医科大学药学院,南宁
市,530021;广西民族医药研究院,南宁市,530001
【正文语种】中文
【中图分类】R284.2;R965.2
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