肥大细胞对THP-1巨噬细胞源性泡沫细胞胆固醇分配和流出的影响优质课件PPT

80
with MCs = 27.1%;
with HDL3+ MCs =7.6%;
60
with compoh compound 48/80+HDL3=19.4%. a:P<0.05 vs. control group;
20
b
b: P<0.001 vs. HDL3 group.
The effect of compound 48/80 on MCs degranulation
A B
实验结果
MCs were stained by toluidine blue (×1000) A，MCs； B，MCs+C48/80.
例数 （n）
组胺含量 （µg/ml）
颗粒释放液
细胞裂解液
组胺释放率 （%）
Groups
TC
FC
CE
control
1092.3± 336.3±31 756±60 65
HDL3
527.3±4 276.9±34 250.4±1
9a
7
MCs
891.3±6 650.1±46 241.2±1
2
b
9b
HDL3+MCs
917.9±1 4c
848.5±16 c
69.4±2 c
Values are
肥大细胞对THP-1巨噬细胞源性泡 沫细胞胆固醇分配和流出的影响
赵战芝 杨永宗
2021/02/01
1
肥大细胞(MCs)
研究背景
一种参与炎症免疫应答反应的炎症细胞。
胞浆内含有大量异染性颗粒（组胺等）。
受刺激时脱颗粒,释放胞浆一系列可溶性介质到细胞外
（compound48/80、oxLDL等可以刺激MCs脱颗粒) 。
2021/02/01
2
促动脉粥样硬化(As)形成
MCs加速oxLDL诱导平滑肌细胞泡沫化。
Compound48/80处理的新西兰白兔和apoE-/鼠主动脉脂纹形成比对照组早，面积大，分布 广。
2021/02/01
3
HDL Metabolism and Reverse Cholesterol Transport
Bile
FC
CE
SR-BI
MC
A-I
?
A-I
LCAT
CE
CE
FC
FC
Nascent ABCA1
Mature
HDL
HDL
胆固醇流出减少
ABC1 = ATP-binding cassette protein 1; A-I = apolipoprotein A-I; CE = cholesteryl ester; FC = free cholesterol; LCAT = lecithin:cholesterol acyltransferase; SR-BI = scavenger receptor class BI
0
0
2
4
6
8
m in
control
HDL3
MCs
HDL3+MCs
C48/80
HDL3+C48/80
The chromatography picture of cholesterol and cholesterol ester in THP-1 macrophage-derived foam cells identified by high performance liquid chromatography.
0
n=5.
%FC or %EC
control HDL3
MCs HDL3+MCs
C48/80 HDL3+C48/80
2021/02/01
9
The effect of MCs on cholesterol efflux from THP-1
macrophage-derived foam cells mediated by HDL3
50
**
40
#
Cholesterol efflux(%)
30
20
10 * 0
*
*
control HDL3
MCs HDL3+MCs HDL3+CC4488//8800
control=2.89%; with HDL3=40.98%; with MCs =2.91% ; with HDL3+ MCs =26.92%; with compound 48/80=3.71%; and with compound 48/80+HDL3=35.23%.
HPLC analysis of THP-1macrophage derived foam cells
CE/TC Ratio:68.7%
Oil red O staining of THP-1macrophage derived foam cells
2021/02/01
6
The effect of MCs on the accumulation of cell cholesterol in THP-1 macrophage-derived foam cells
The effect of MCs on the distribution of cell free and esterified cholesterol in THP-1 macrophage-derived
foam cells
120
FC
EC
control (alone)=69.2%;
100
with HDL3=47.5%;
5
0.340±0.005
0.077±0.021
81.53±4.51
MCs histamine release percentage
Formation of THP-1 macrophage-derived foam cells
FC
CE
FC，free cholesterol CE，cholesterol ester
8c0: P<0.05 vs. H0aDL3 group.
2021/02/01
7
D AD 1A,Sig=210,30R ef=360,100(M \94BZ0100.D )
m AU
2.595
800
cholesterol
700
Cholesterol standard
600
500
400
300
200
100
C48/80
repre8s0en7t.e3d±a6s（
x343s.）1±mg36cho4le6s4te.r2o±l/g1protein,
(
n=5
).
a:P<0.05
HDL3+b:CP4<80/.05
vv3ss1..c4co.o83nn±ttrroo1l,lHgDr2oL5u33p+.;2M±C1s1grou6p1;.13±8