A Novel Approach of Intra-Prediction Mode Selection in H264在H.264帧内预测模式选择的新方法-PPT课件

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《Broadcasting, IEEE Transactions on》期刊第3页200条数据

《Broadcasting, IEEE Transactions on》期刊第3页200条数据

《Broadcasting, IEEE Transactions on》期刊第3页200条数据https:///academic-journal-foreign_broadcasting-ieee-transactions_info_128_1/1.《A New Blind SLM Scheme With Low Decoding Complexity for OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html2.《Implementation and Co-Simulation of Hybrid Pilot-Aided Channel Estimation With Decision Feedback Equalizer for OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html3.《A Depth-Aware Character Generator for 3DTV》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html4.《Novel End-to-End Quality of Service Provisioning Algorithms for Multimedia Services in Virtualization-Based Future Internet》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html5.《FPGA Design and Performance Evaluation of a Pulse-Based Echo Canceller for DVB-T/H》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html6.《On the Provisioning of Mobile Digital Terrestrial TV Services to Vehicles With DVB-T》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html7.《Reception Quality Prediction in a Single Frequency Network for the DTMB Standard》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html8.《Signal-to-Noise Ratio Estimation Algorithm for Advanced DVB-RCS Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html9.《Augmented Data Transmission Based on Low Density Parity Check Code for the ATSC Terrestrial DTV System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html10.《Adaptive Digital Predistortion for Wideband High Crest Factor Applications Based on the WACP Optimization Objective: A Conceptual Overview》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html11.《Initial-Estimation-Based Adaptive Carrier Recovery Scheme for DVB-S2 System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html12.《Quantifying Subjective Quality Evaluations for Mobile Video Watching in a Semi-Living Lab Context》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html13.《Perceived 3D TV Transmission Quality Assessment: Multi-Laboratory Results Using Absolute Category Rating on Quality of Experience Scale》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html14.《Efficient Pilot Patterns and Channel Estimations for MIMO-OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html15.《Coding Distortion Elimination of Virtual View Synthesis for 3D Video System: Theoretical Analyses and Implementation》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html16.《An Efficient Nonlinear Companding Transform for Reducing PAPR of OFDM Signals》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html17.《Saliency Inspired Full-Reference Quality Metrics for Packet-Loss-Impaired Video》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html18.《Improved CIR-Based Receiver Design for DVB-T2 System in Large Delay Spread Channels: Synchronization and Equalization》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html19.《High Power Amplifier Pre-Distorter Based on Neural-Fuzzy Systems for OFDM Signals》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html20.《Performance Analysis of Inter-Layer Prediction in Scalable Video Coding Extension of H.264/AVC》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html21.《Study of Rating Scales for Subjective Quality Assessment of High-Definition Video》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html22.《Planning Factors for Digital Local Broadcasting in the 26 MHz Band》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html23.《Peak-to-Average Power Ratio Reduction of OFDM Signals Using PTS Scheme With Low Computational Complexity》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html24.《Feedback Cancellation for T-DMB Repeaters Based on Frequency-Domain Channel Estimation》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html25.《Efficient Multi-Reference Frame Selection Algorithm for Hierarchical B Pictures in Multiview Video Coding》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html26.《Performance Comparisons and Improvements of Channel Coding Techniques for Digital Satellite Broadcasting to Mobile Users》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html27.《Burst-Aware Dynamic Rate Control for H.264/AVC Video Streaming》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html28.《Helicopter-Based Digital Electronic News Gathering (H-DENG) System: Case Study and System Solution》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html29.《Transmit Diversity for TDS-OFDM Broadcasting System Over Doubly Selective Fading Channels》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html30.《Interference Cancellation Techniques for Digital On-Channel Repeaters in T-DMB System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html31.《Field Measurements of EM Radiation From In-House Power Line Telecommunications (PLT) Devices》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html32.《A Novel Scheme of Joint Channel and Phase Noise Compensation for Chinese DTMB System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html33.《Path Loss Prediction for Mobile Digital TV Propagation Under Viaduct》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html34.《Efficient Motion Vector Interpolation for Error Concealment of H.264/AVC》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html35.《3D-TV Content Creation: Automatic 2D-to-3D Video Conversion》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html36.《A Novel Rate Control Technique for Multiview Video Plus Depth Based 3D Video Coding》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html37.《The Effect of Crosstalk on the Perceived Depth From Disparity and Monocular Occlusions》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html38.《Semi-Automatic 2D-to-3D Conversion Using Disparity Propagation》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html39.《Display-Independent 3D-TV Production and Delivery Using the Layered Depth Video Format》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html40.《3DTV Roll-Out Scenarios: A DVB-T2 Approach》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html41.《PAPR Reduction Using Low Complexity PTS to Construct of OFDM Signals Without Side Information》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html42.《Quality-Oriented Multiple-Source Multimedia Delivery Over Heterogeneous Wireless Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html43.《Efficient PAPR Reduction in OFDM Systems Based on a Companding Technique With Trapezium Distribution》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html44.《Objective Video Quality Assessment Methods: A Classification, Review, and Performance Comparison》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html45.《Pixel Interlacing Based Video Transmission for Low-Complexity Intra-Frame Error Concealment》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html46.《Fountain Codes With PAPR Constraint for Multicast Communications》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html47.《RF Watermark Backward Compatibility Tests for the ATSC Terrestrial DTV Receivers》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html48.《61st Annual IEEE Broadcast Symposium — Save the Date》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html49.《Evaluation of Asymmetric Stereo Video Coding and Rate Scaling for Adaptive 3D Video Streaming》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html50.《Stereoscopic Perceptual Video Coding Based on Just-Noticeable-Distortion Profile》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html51.《A Depth Information Based Fast Mode Decision Algorithm for Color Plus Depth-Map 3D Videos》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html52.《3D-TV Production From Conventional Cameras for Sports Broadcast》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html53.《A Digital Blind Watermarking for Depth-Image-Based Rendering 3D Images》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html54.《Object-Based 2D-to-3D Video Conversion for Effective Stereoscopic Content Generation in 3D-TV Applications》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html55.《3D-TV Content Storage and Transmission》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html56.《New Depth Coding Techniques With Utilization of Corresponding Video》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html57.《3DTV Broadcasting and Distribution Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html58.《Boundary Artifact Reduction in View Synthesis of 3D Video: From Perspective of Texture-Depth Alignment》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html59.《Stereoscopic 3D-TV: Visual Comfort》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html60.《A Novel Inpainting-Based Layered Depth Video for 3DTV》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html61.《3D-TV R&D Activities in Europe》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html62.《A Directional-View and Sound System Using a Tracking Method》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html63.《Joint Maximum Likelihood Estimation of Carrier and Sampling Frequency Offsets for OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html64.《Perceptual Issues in Stereoscopic Signal Processing》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html65.《Performance Evaluation of Multimedia Content Distribution Over Multi-Homed Wireless Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html66.《The Relationship Among Video Quality, Screen Resolution, and Bit Rate》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html67.《Corrections to “Efficient Motion Vector Interpolation f or Error Concealment of H.264/AVC”》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html68.《PAPR Reduction of OFDM Signals by PTS With Grouping and Recursive Phase Weighting Methods》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html69.《Improve the Performance of LDPC Coded QAM by Selective Bit Mapping in Terrestrial Broadcasting System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html70.《The Importance of Visual Attention in Improving the 3D-TV Viewing Experience: Overview and New Perspectives》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html71.《Co-Channel Analog Television Interference in the TDS-OFDM-Based DTTB System: Consequences and Solutions》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html72.《Prediction and Transmission Optimization of Video Guaranteeing a Bounded Zapping-Delay in DVB-H》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html73.《IBC2011 Experience the Future》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html74.《61st Annual IEEE Broadcast Symposium — Save the Date》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html75.《A 2$times$2 MIMO DVB-T2 System: Design, New Channel Estimation Scheme and Measurements With Polarization Diversity》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html76.《A Pilot Symbol Pattern Enabling Data Recovery Without Side Information in PTS-Based OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html77.《Efficient Incremental Raptor Decoding Over BEC for 3GPP MBMS and DVB IP-Datacast Services》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html78.《Spatio-Temporally Consistent Novel View Synthesis Algorithm From Video-Plus-Depth Sequences for Autostereoscopic Displays》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html79.《IBC2011 Experience the Future》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html80.《Evaluation of Stereoscopic Images: Beyond 2D Quality》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html81.《An Evaluation of Parameterized Gradient Based Routing With QoE Monitoring for Multiple IPTV Providers》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html82.《Three-Dimensional Displays: A Review and Applications Analysis》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html83.《Guest Editorial Special Issue on 3D-TV Horizon: Contents, Systems, and Visual Perception》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html84.《LIVE: An Integrated Production and Feedback System for Intelligent and Interactive TV Broadcasting》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html85.《$MS^{2}$ : A New Real-Time Multi-Source Mobile-Streaming Architecture》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html86.《A Reverse-Order Scheduling Scheme for Broadcasting Continuous Multimedia Data Over a Single Channel》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html87.《An Automatic Recommendation Scheme of TV Program Contents for (IP)TV Personalization》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html88.《Irregular Mapping and its Application in Bit-Interleaved LDPC Coded Modulation With Iterative Demapping and Decoding》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html89.《Time Diversity in Mobile DVB-T2 Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html90.《An Audio Quality Evaluation of Commercial Digital Radio Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html91.《Spectral Geometry Image: Image Based 3D Models for Digital Broadcasting Applications》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html92.《Novel Approach to Support Multimedia Services Over DTMB System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html93.《Collaborative Content Fetching Using MAC Layer Multicast in Wireless Mobile Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html94.《Energy-Efficient Transmission for Protection of Incumbent Users》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html95.《Normalization Factor of Hierarchically Modulated Symbols for Advanced T-DMB System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html96.《Pooling-Based Intra Prediction Mode Coding for Mobile Video Applications》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html97.《A Suboptimal Tone Reservation Algorithm Based on Cross-Entropy Method for PAPR Reduction in OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html98.《A Measurement Method of the DTMB Modulator》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html99.《Interference Elimination for Chinese DTMB System With Transmit Diversity》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html100.《61st Annual IEEE Broadcast Symposium》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html101.《IBC2011 Experience the Future》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html102.《A Low-Complexity SLM Scheme Using Additive Mapping Sequences for PAPR Reduction of OFDM Signals》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html103.《Illumination-Sensitive Background Modeling Approach for Accurate Moving Object Detection》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html104.《Coordinating Allocation of Resources for Multiple Virtual IPTV Providers to Maximize Revenue》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html105.《Inter-Sequence Error Concealment Techniques for Multi-Broadcast TV Reception》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html106.《Performance Evaluation of the DVB-RCT Standard for Interactive Services》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html107.《An Efficient Predistorter Design for Compensating Nonlinear Memory High Power Amplifiers》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html108.《Accurate BER Analysis of OFDM Systems Over Static Frequency-Selective Multipath Fading Channels》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html109.《A Frame-Related Approach for Performance Improvement of MPE-FEC in DVB-H》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html110.《Balanced Multiple Description Coding for 3D DCT Video》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html111.《Performance Validation of the DVB-SH Standard for Satellite/Terrestrial Hybrid Mobile Broadcasting Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html112.《An Improved Tone Reservation Scheme With Fast Convergence for PAPR Reduction in OFDM Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html113.《Metaheuristic Procedure to Optimize Transmission Delays in DVB-T Single Frequency Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html114.《Adaptive Resource Allocation for MIMO-OFDM Based Wireless Multicast Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html115.《An Analytical Approach for Performance Evaluation of Hybrid (Broadcast/Mobile) Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html116.《Cost-Aware Wireless Data Broadcasting》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html117.《Subspace-Based Semi-Blind Channel Estimation in Uplink OFDMA Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html118.《Performance of the Consumer ATSC-DTV Receivers in the Presence of Single or Double Interference on Adjacent/Taboo Channels》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html119.《A Cooperative Cellular and Broadcast Conditional Access System for Pay-TV Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html120.《A Narrow-Angle Directional Microphone With Suppressed Rear Sensitivity》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html121.《Peak-to-Average Power Ratio Reduction in OFDM Systems Using All-Pass Filters》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html122.《Development of Advanced Terrestrial DMB System》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html123.《HDTV Subjective Quality of H.264 vs. MPEG-2, With and Without Packet Loss》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html124.《Estimation of RF Electromagnetic Levels Around TV Broadcast Antennas Using Fuzzy Logic》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html125.《Statistical Multiplexing for Digital Audio Broadcasting Applications》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html126.《A Composite PN-Correlation Based Synchronizer for TDS-OFDM Receiver》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html127.《Application of Quantum-Inspired Evolutionary Algorithm to Reduce PAPRof an OFDM Signal Using Partial Transmit Sequences Technique》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html128.《Improved Decoding Algorithm of Bit-Interleaved Coded Modulation for LDPC Code》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html129.《Precoding for PAPR Reduction of OFDM Signals With Minimum Error Probability》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html130.《Network Design and Field Application of ATSC Distributed Translators》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html131.《On the Channel and Signal Cross Correlation of Downlink and Uplink Mobile UHF DTV Channels With Antenna Diversity》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html132.《Performance Evaluation of TV Over Broadband Wireless Access Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html133.《IBC2010 Experience the State-of-the-Art》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html134.《Peak-to-Average Power Ratio Reduction of OFDM Signals With Nonlinear Companding Scheme》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html135.《Motion-Compensated Frame Rate Up-Conversion—Part I: Fast Multi-Frame Motion Estimation》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html136.《Comments on Equation (4) in “Single Frequency Networks in DTV”》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html137.《Motion-Compensated Frame Rate Up-Conversion—Part II: New Algorithms for Frame Interpolation》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html138.《A Novel Equalization Scheme for ZP-OFDM System Over Deep Fading Channels》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html139.《A Synchronization Design for UWB-Based Wireless Multimedia Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html140.《Frequency Domain Decision Feedback Equalization for Uplink SC-FDMA》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html141.《A 2 2 MIMO DVB-T2 System: Design, New Channel Estimation Scheme and Measurements With Polarization Diversity》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html142.《Impact of the Receive Antenna Arrays on Spatio-Temporal Availability in Satellite-to-Indoor Broadcasting》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html143.《Reducing Channel Zapping Time in IPTV Based on User's Channel Selection Behaviors》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html144.《On the Methodology for Calculating SFN Gain in Digital Broadcast Systems》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html145.《Statistical Multiplexing of Upstream Transmissions in DOCSIS Cable Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html146.《Bit-Rate Allocation for Broadcasting of Scalable Video Over Wireless Networks》原文链接:https:///academic-journal-foreign_broadcasting-ieee-transactions_thesis/020*********.html147.《Full-Reference Video Quality Metric for Fully Scalable and Mobile SVC Content》。

女性膀胱过度活动症患者的逼尿肌过度活动尿动力学判定

女性膀胱过度活动症患者的逼尿肌过度活动尿动力学判定
承德市科学技术研究与发展计划项 目(编号 :201231 15)
患 者 的应用 价值 ,现 报告如 下 。
1 资料 与 方法
1.1 一般 资料 2012年 9月 至 2014年 9月选 择 在 承 德 医学 院附属 医 院诊治 的女性 OAB患者 89例作 为 观察 组 ,纳入标 准 :以尿 频 、尿急就 诊 的女性 患 者 ; 临床尿 流动 力学 检查 诊 断证 实 为 OAB;国 际前 列 腺 症 状 评 分 (IPSS)>12分 ;最 大 尿 流率 (MFR)<13 mL/s;残 余 尿 量 (residual urine volume,RUV)>60 mL。排 除标 准 :泌 尿 系 感 染 患 者 ;有 糖 尿 病 及 心 脑 血 管疾 病者 ;神 经 源性 膀 胱 、尿 道 狭 窄 等 患 者 ;妊 娠 与 哺乳 期妇 女 ;尿路 结 石 及 既往 泌 尿 生 殖 道 手 术 史 患 者 。年龄 27~79岁 ,平 均 (56.22-4-5.29)岁 ;病 程 1—11年 ,平 均 (4.11±0.92)年 ;体 质指 数 为 (23.11±2.94)kg/m ;IPSS评 分 为 (23.87±7.29) 分 。同期选 择 因 肾结 石住 院 ,经 压 力 一流 率 测 定 膀 胱 功 能正 常 ,无 尿频 、尿 急等下 尿路 症状 的成 年女 性 89例 作 为 对 照 组 ,年 龄 26~77岁 ,平 均 (56.09± 4.11)岁 ;体 质 指数 为 (24.09±3.19)kg/m 。 每 例 患 者 均有知 情 同意 书 ,并 获得 医 院伦理 委员会 同意 , 两 组 患者 的年龄 、体 质 指 数 等 对 比差异 无 统 计 学 意 义 (P>0.05)。 1.2 尿 动 力 学检 查 方法 选 择 丹 麦 Duet尿动 力 学 检 测仪 ,患 者检查 前应 排净 大便 ,按 照标 准方 法进 行

培哚普利吲达帕胺联合硝苯地平治疗单药治疗未达标高血压患者的效果及安全性分析

培哚普利吲达帕胺联合硝苯地平治疗单药治疗未达标高血压患者的效果及安全性分析

- 41 -systematic review[J]. HPB (Oxford),2018,20(9):786-794.[11]陈晨.胆囊切除术后综合征发病机制及治疗的研究进展[J].疑难病杂志,2021,20(9):963-972.[12]张伟,柳鹏飞,安振涛,等.胆囊切除术后综合征中医治法及方药规律研究[J].中国医药导报,2020,17(36):150-154.[13]孙改萍,惠建萍,刘海涛.中医药治疗胆囊切除术后腹泻临证思路撷要[J].江苏中医药,2019,51(7):45-47.[14]牛璐璐,闫彦峰,柴辉,等.刘国安治疗胆囊切除术后综合征经验[J].中国中医药信息杂志,2018,25(12):119-121.[15]张杰,唐勇,张晓娟,等.肝脾建中思想及临床应用[J].安徽中医药大学学报,2019,38(6):28-30.[16]敖雪仁,廖聪,吴剑纯,等.柴芩止泻方治疗胆囊切除术后胆热脾寒型腹泻36例[J].环球中医药,2022,15(8):1473-1476.[17] PONNUSAMY S,LATTMANN E,LATTMANN P,et al.Novel, isoform-selective, cholecystokinin a receptor antagonist inhibits colon and pancreatic cancers in preclinical models through novel mechanism of action[J]. Oncol Rep,2016,35(4):2097-2106.[18] YAKABI S,WANG L,KARASAWA H,et al. VIP isinvolved in peripheral CRF-induced stimulation of propulsive colonic motor function and diarrhea in male rats[J]. Am J Physiol Gastrointest Liver Physiol,2018,314(5):G610-G622.[19] RYCHLIK A,GONKOWSKI S,CA ŁKA J,et al. Vasoactiveintestinal polypeptide (VIP) in the intestinal mucosal nerve fibers in dogs with inflammatory bowel disease[J]. Animals (Basel),2020,10(10):1759-1760.(收稿日期:2023-07-07)①黄河三门峡医院 河南 三门峡 472000培哚普利吲达帕胺联合硝苯地平治疗单药治疗未达标高血压患者的效果及安全性分析陈鹏①【摘要】 目的:探究和分析单药治疗未达标高血压患者应用培哚普利吲达帕胺联合硝苯地平治疗的效果和安全性。

应用荧光实时定量PCR方法检测重组慢病毒滴度及其感染效率(精品)

应用荧光实时定量PCR方法检测重组慢病毒滴度及其感染效率(精品)

生命科学研究2009年慢病毒载体是目前应用最广泛的基因运载工具之一,在基因治疗研究和转基因动物的制备中已显示出其广阔的应用前景.慢病毒载体是一种反转录病毒载体,其中以人类免疫缺陷性病毒(HIV -1)载体研究最为深入.与传统的小鼠白血病病毒载体(MuLV )偏爱整合入基因5′端相比,慢病毒载体均匀分布于基因组内,从而降低了其激活原癌基因的几率[1~3].重组慢病毒载体既能感染分裂期细胞,也能感染非分裂期细胞[4].它可携带较大的外源基因(约8kb 左右)并稳定整合和表达[5],加之其诱发的宿主免疫反应相对较小[6],使得重组慢病毒载体具有较为广阔的应用应用荧光实时定量PCR 方法检测重组慢病毒滴度及其感染效率马海燕,方彧聃,张敬之*(上海交通大学医学院,上海市儿童医院上海市医学遗传研究所,中国上海200040)摘要:慢病毒载体已经广泛应用于动物模型中基因治疗的研究和转基因动物的制备,而准确地测定重组慢病毒的滴度和感染效率是其关键步骤.通过荧光实时定量PCR 的方法定量分析重组慢病毒的颗粒数以及病毒的活性滴度,并以GFP 报告基因的方法作为对照来验证定量PCR 方法的准确性.研究结果显示,应用荧光实时定量PCR 法与GFP 报告基因法测定得到的病毒活性滴度成正相关,而且前者可以更加准确地测定病毒滴度和病毒感染效率.关键词:慢病毒载体;荧光实时定量PCR ;病毒滴度;整合拷贝数;感染效率中图分类号:Q331文献标识码:A文章编号:1007-7847(2009)05-0394-05A Novel Method for the Determination of Recombinant LentiviralTiter and Infectivity by qRT -PCRMA Hai -yan ,FANG Yu -dan ,ZHANG Jing -zhi *(School of Medicine ,Shanghai Jiaotong University ,Shanghai Children ’s Hospital ,Shanghai Institute of Medical Genetics ,Shanghai 200040,China )收稿日期:2009-03-18;修回日期:2009-05-16基金项目:国家高技术研究发展计划项目(2007AA021206);国家自然科学基金资助项目(30870943);上海市自然科学基金资助项目(08ZR1412100)作者简介:马海燕(1983-),女,山东淄博人,硕士研究生,主要从事病毒载体在转基因动物制备中的应用研究;*通讯作者:张敬之(1959-),男,上海人,上海交通大学医学遗传研究所副教授,博士,主要从事分子病毒学研究,Tel :021-********,E -mail :********************.Abstract :Lentiviral vector is being widely used in the study of gene therapy in animal models and in generating transgenic animals.However ,determination of lentiviral particles and their infectivity is essential before their being used.Such a requirement can be accurately achieved by qRT -PCR.Refered by infectious units got from GFP reporter assay ,it showed a positive correlation between the two approaches.A reliable ,accurate and rapid method is therefore established for the determination of the recombinant lentiviral titer and the infectivity.Key words :lentiviral vector ;qRT -PCR ;viral titer ;integration copy number ;infectivity(Life Science Research ,2009,13(5):394~398)第13卷第5期生命科学研究Vol.13No.52009年10月Life Science Research Oct.2009第5期前景.无论何种目的使用重组慢病毒,都有必要准确地检测病毒滴度.目前,重组慢病毒滴度的检测方法有:依赖于报告基因的GFP荧光检测法、检测慢病毒外壳蛋白p24的抗原-抗体法(ELISA 法)和检测其逆转录酶活性的酶学法等.这些方法多存在耗时、费力、检测成本较高、病毒用量多、不适于非报告基因载体等缺点.所以,建立一种快速、简便、准确的慢病毒滴度检测方法,是非常有必要的.在此,我们介绍一种利用实时定量PCR测定重组慢病毒滴度的方法.该方法通过在载体的长末端重复序列区(LTR)设计定量引物,利用荧光实时定量PCR测定重组慢病毒中LTR拷贝数来测定病毒颗粒数和有效感染的病毒颗粒数.通过与GFP报告基因测定法的比较验证,证明该测定方法准确可靠.并且,通过慢病毒颗粒数与实际有活力病毒滴度的比较,可以计算得到重组慢病毒感染效率.多次重复实验证明,该方法具有快速、准确的优点,非常适用于非报告基因载体的病毒滴度及其感染效率的测定.1材料与方法1.1材料组成慢病毒载体的3个质粒FUGW(即含eGFP基因的转基因质粒),△8.9(编码结构和非结构蛋白基因质粒)和VSVG(外壳蛋白质粒)由美国Marine Medical Center Research Institute王征宇博士惠赠;293T细胞(人胚肾细胞)购自美国ATCC(American Type Culture Collection)细胞库;ProFection购自Promega公司;胎牛血清(FBS)、培养液和Hanks液及Salmon Sperm DNA 购自Gibco BRL;STE配方为10mmol/L Tris pH 7.6,1mmol/L EDTA pH8.0,0.1mol/L NaCl.1.2FUGW病毒的制备当293T细胞长至70%饱和度时,用磷酸钙法转染,按照Promega公司的ProFection Kit说明书操作,其中FUGW15μg,△8.910μg,VSVG7.5μg,转染6h后换完全培养液(含10% FBS的DMEM),并在37℃,5%CO2培养约60h.上述病毒培养上清液经离心、过滤后,50000g 超速离心1.5h后弃上清,在病毒沉淀上加少量Hanks液,获得病毒浓缩液,-80℃保存待用.1.3病毒RNA的提取取2μL病毒浓缩液进行DNase处理,体系中加入5μL10×DNase I Buffer、2μL DNase I (5U/uL,Takara Bio Inc,Shiga,Japan)、2μL RNase inhabitor(Takara Bio Inc.,Shiga,Japan),用DEPC水定容至50μL反应体系,37℃45 min.DNase处理后的混合液,加入350μL STE、20μL10%SDS和5μL蛋白酶K(20g/L,AMRESCO,Solon,OH),56℃15min水解.最后等体积酚、氯仿抽提,两倍体积无水乙醇沉淀,冻干后,20μL DEPC水溶解.1.4反转录反应病毒RNA在酚/氯仿抽提、无水乙醇沉淀前,需经过DNase I处理,以避免DNA污染.根据产品说明书,取1μg RNA、20pmol RT-PCR 下游引物(5′-GAGAGCTCCCAGGCTCAGATC-3′)、2μL5×RT Buffer、1μL MLV酶(Takara Bio Inc,Shiga,Japan)、水补足至10μL体系,37℃1h.1.5实时定量PCR分析病毒颗粒数为了测定制备的慢病毒的病毒颗粒数,应用实时定量PCR测定病毒LTR拷贝数.其中,引物和探针序列见表1.在反应体系中,引物900 nmol/L,探针250nmol/L,2.5μL10×Ex-Buffer,15μmol Mg2+,2.5μmol dNTP,1U ExTaqE,5μL样本,总反应体系为25μL体积.实时定量PCR仪(Corbett Life Science RG-3000,Sidney,Australia)上反应:95℃5min变性,95℃30s,59℃30s,40个循环,59℃520nm处检测荧光值.软件分析荧光检测数据.1.6不同剂量FUGW病毒感染293T细胞取病毒浓缩液,按10倍稀释法,取0.1、0.01、0.001μL病毒浓缩液(即用实时定量PCR 检测法,定量慢病毒颗粒数为:6.32×107、6.32×106、6.32×105),用含有8mg/L Polybrene促感染(Sigma-Aldrich,Inc,St.Louis,MO)且不含血清的培养液逐级稀释后,感染293T细胞(1.5×106/孔),37℃2h.然后,加入完全培养液培养细胞2d,表1实时定量PCR引物及探针序列Table1The sequences of primer and probe of Real-time PCRLTR-FLTR-PLTR-ProbePrimer Sequences5′-ACAGCCGCCTAGCATTTCAT-3′5′-GAGAGCTCCCAGGCTCAGATC-3′5′-ACATGGCCCGAGAGCTGCATCC-3′马海燕等:应用荧光实时定量PCR方法检测重组慢病毒滴度及其感染效率395生命科学研究2009年图1定量PCR 反应荧光强度曲线Fig.1Fluorescence intensity curve of Real -time PCR图2定量PCR 标准曲线Fig.2Standard curve of Real -time PCR至荧光显微镜下观测绿色荧光蛋白表达情况.1.7病毒感染细胞内DNA 的提取细胞经2d 培养后,用胰酶将细胞消化,收集入1.5mL EP tube 中,200g ,5min ,将细胞沉淀下来.加入200μL STE 、20μL 10%SDS 、10μL 蛋白酶K ,混匀后,37℃4h.加入等体积酚,振荡混匀,15000g ,离心12min ;吸取上清,加入等体积的氯仿,振荡混匀,15000g 离心6min ;吸取上清,加入1/10体积3mol/mL NaAc ,两倍体积的无水乙醇,-20℃沉淀1h 以上;混合液15000g ,4℃离心20min ;弃上清,沉淀风干,100μL TE 溶解.1.8实时定量PCR 测定重组载体整合拷贝数为了测定被感染的293T 细胞内重组载体的整合,用实时定量PCR 检测上述被抽提的基因组DNA 中LTR 的拷贝数.具体方法同1.5.2结果2.1实时定量PCR 检测病毒颗粒数本实验中,定量PCR 引物设计在LTR 区,由于一个慢病毒含有两个病毒基因拷贝,因此,在计算病毒颗粒数时,LTR 拷贝数除以2即为病毒颗粒数.应用实时定量PCR 检测到病毒LTR 拷贝数为1.26×1012/mL ,定量PCR 反应荧光强度曲线及标准曲线见图1、图2.通过计算,得到病毒颗粒数为6.32×1011/mL .此时计算得到的病毒颗粒数为所有收集到的病毒颗粒总数,既包括有感染效力的病毒,也包括无感染效力的病毒.为了测定制备得到的病毒实际滴度(即单位体积内有感染效力的病毒颗粒数),我们将不同剂量病毒感染细胞,分别用GFP 报告基因法和定量PCR 法测定病毒滴度.2.2实时定量PCR 检测病毒载体整合拷贝数,计算病毒滴度取病毒浓缩液,按照10倍稀释,分别取0.1、0.01、0.001μL 病毒浓缩液感染1.5×106293T 细胞,感染2d 后,应用实时定量PCR 检测不同病毒量感染的细胞DNA 中LTR 整合拷贝数,结果显示,0.1、0.01、0.001μL 病毒感染的细胞中外源基因整合的拷贝数分别为5.32×106、9.28×105、4.48×104.定量PCR 的系统参数为:R =0.99,R ∧2=0.99,Efficiency =1.01,各参数值表明实验检测的准确性和可信性.当一个病毒感染细胞并将外源基因整合入基因组后,由于其末端发生跳跃过程,使得每条DNA 单链上含有两个完整的LTR ,因此,在被整合的细胞基因组,一个慢病毒颗粒=LTR 拷贝数/4.通过计算,得到病毒滴度为(1.59±0.64)×1010IU /mL .2.3GFP 报告基因检测法测定病毒滴度同时将0.1、0.01、0.001μL 病毒浓缩液感染2d 后的细胞,置于荧光显微镜下观测.镜检结果显示,GFP 阳性细胞数逐级递减,0.1、0.01、0.001μL 病毒量感染的细胞中,GFP 阳性率分别为52%、6%、0.5%,呈现较好的倍比关系(图3).通过公式:病毒滴度=感染细胞数×GFP 阳性率×病毒稀释倍数÷病毒量,得出病毒滴度为(8.10±0.79)×109IU/mL .2.4GFP 报告基因检测法与定量PCR 检测法所得病毒滴度比较,验证定量PCR 检测法准确性通过比较GFP 报告基因检测法和实时定量510152025303510^-110^-210^-3N o r m f l u o r eCycle numberThreshold·····10^410^510^610^710^810^940302010R =0.99980R ^2=0.99960Efficiency=0.96M =0.293B =13.087Concentration (Copy number )T h r e s h o l d c y c l e396第5期注:病毒感染293T 细胞数为1.5×106.Notes :293T cells in each well are:1.5×106.图3逐级稀释的慢病毒感染293T 细胞后GFP 的表达情况(A )0.1μL 病毒感染细胞;(B )0.01μL 病毒感染细胞;(C )0.001μL 病毒感染细胞.Fig.3GFP expression in infected 293T cells after 10fold dilution (A )293T cells infected by 0.1μL virus ;(B )293T cells infected by 0.01μL virus ;(C )293T cells infected by 0.001μL virus.PCR 检测法(表2),可以看出,与我们预期结果一致,实时定量PCR 法检测结果与GFP 报告基因检测法检测结果成正相关.多次重复实验结果均表明,两者的检测结果成稳定的正相关性.而且,实时定量PCR 检测法不依赖GFP 蛋白正常表达,对整合入宿主基因组但不能正常表达的病毒仍然能够在其检测范围之内,因此,实时定量PCR 的检测结果更接近实际值,能够更加准确的检测病毒滴度.(A )(B )(C)表2GFP 报告基因检测法与定量PCR 检测法测得病毒滴度比较Table 2Comparison of lentivirus titer determined by GFP reporter assay and qRT -PCR approachDose of viral particles6.3×107 6.3×106 6.3×105GFP reporter assay Percentage of GFP +cells Infectious units qRT -PCR assayInfectious units52.0%7.8×1051.3×1066.0%9.0×1042.3×1050.5%7.5×1031.1×1042.5荧光实时定量PCR 法测定病毒实际感染效率通过计算公式:感染效率=有效感染病毒数/感染细胞总病毒颗粒数×100%,得到该实验中慢病毒的感染效率为:2.65±1.07%.3讨论慢病毒载体的研究,目前以HIV -1最为深入.慢病毒载体构建的基本原理是将HIV -1基因组中的基本骨架与编码其功能蛋白相分离,分别改建成载体质粒和表达包装蛋白的质粒,并将两种成分共转染入细胞,从中获得只有一次感染能力而没有复制能力的HIV -1载体假病毒[7],从而提高了其应用的安全性.近年来,越来越多的研究者利用慢病毒载体系统作为在动物模型研究基因治疗的导入系统,并取得良好的效果[8~10].与此同时,利用慢病毒载体介导制备转基因动物的研究也得到发展,慢病毒载体介导成功制备了转基因小鼠[11,12]、转基因猪[13]、转基因牛[14]等动物,为基因工程领域的研究奠定基础.由于慢病毒载体的基因转导效率主要取决于病毒滴度,这就使得慢病毒滴度及其感染效率的检测变得很重要.目前,通行的慢病毒滴度检测方法有:1)p24等抗原酶联检测法(p24ELISA 方法).其缺点是:商业化的ELISA 试剂盒往往太贵,约6000元人民币一盒.而且蛋白含量检测结果不能直接反映其拷贝数;2)使用报告基因系统,无法检测其真实颗粒数及不携带报告基因的假病毒滴度;3)检测逆转录酶活性,用量马海燕等:应用荧光实时定量PCR 方法检测重组慢病毒滴度及其感染效率397生命科学研究2009年大,操作复杂及准确性差.本文所阐述的通过实时定量PCR法测定LTR拷贝数来检测病毒滴度的方法,能在提高慢病毒滴度检测准确性的同时,缩短检测时间、减少检测成本.相比传统检测方法,实时定量PCR 检测法有以下特点:1)由于本检测方法中,实时定量PCR的检测引物设计在慢病毒载体的LTR区,因此检测不依赖于所携带的外源基因;2)传统的GFP报告基因检测方法依赖绿色荧光蛋白的表达,对于外源基因整合入宿主基因组中但由于基因沉默而未能表达GFP蛋白的细胞无法检测,致使滴度测定不能准确地反应病毒的感染效率;而实时定量PCR检测法不依赖GFP报告基因的功能表达,因此其准确程度更高;3)报告基因检测法,因其感染和表达效率随宿主细胞而异,而利用实时定量PCR方法,直接检测病毒的颗粒数和整合入宿主基因组内的外源基因的拷贝数,从而大大提高了其检测的准确性.目前,临床上及实验室所应用的定量PCR方法,通常是检测整合入宿主细胞的基因拷贝数,因此,是对有活力病毒滴度的测定.而本文所介绍的方法,是通过直接裂解病毒,利用荧光实时定量PCR检测总的病毒颗粒数,并结合传统的慢病毒活力滴度的检测方法,对病毒颗粒的感染效率进行检测.所以本方法更适于被应用于研究病毒制备、感染过程中各因素对病毒感染效率的影响.病毒浓缩和感染过程中,由于受超离的压力、反复冻融、受体细胞易感性、病毒自身半衰期等诸多因素影响,使得有效的病毒数要低于其总颗粒数.为了确定实验中病毒的用量,需要预测病毒的实际感染效率.利用本文所介绍的实时定量PCR方法,检测慢病毒颗粒数及有活性的病毒滴度,通过计算有活力的病毒颗粒和总病毒颗粒的比值,我们可以得到每次制备的病毒的感染效率.在我们的实验中,所得到的病毒在293T细胞的实际感染效率为4%左右.而且多次实验表明,反复冻融对病毒感染效率具有很大影响.病毒实际感染效率的测定,为我们在进行具体实验中确定病毒用量具有实际指导意义.经本实验室多次重复试验,结果均表明荧光实时定量PCR法是一种高效、准确、快速的检测重组慢病毒滴度及其感染效率的方法,为重组慢病毒载体的应用奠定了基础.致谢:感谢任兆瑞教授对本文的悉心指导.参考文献(References):[1]WU X,LI Y,CRISE B,et al.Transcription start regions inthe human genome are favored targets for MLV integration[J].Science,2003,300:1749-1751.[2]DEPALMA M,MONTINI E,SANTONIDESIO F R,et al.Promoter trapping reveals significant differences in integrationsite selection between MLV and HIV vectors in primaryhematopoietic cells[J].Blood,2005,105(6):2307-2315. [3]SCHRODER A R,SHINN P,CHEN H,et al.HIV-1integration in the human genome favors active genes and localhotspots[J].Cell,2002,110:521-529.[4]JAKOBSSON J,ERICSON C,JANSSON M,et al.Targetedtransgene expression in rat brain using lentiviral vectors[J].Neurosci Res,2003,73(6):876.[5]张敬之,郭歆冰,谢书阳,等.用慢病毒载体介导产生绿色荧光蛋白(GFP)转基因小鼠[J].自然科学进展(ZHANGJing-zhi,GUO Xin-bin,XIE Shu-yang,et al.Production oftransgenic mice carrying green fluorescence protein gene by alentiviral vector-mediated approach[J].Progress in NatureScience),2006,16(8):827-832.[6]STEWART S A,OYLOCHOOM D M,PALLISER D,et al.Lentivims-delivered stable gene silencing by RNAi in primarycells[J].RNA,2003,9(4):493-501.[7]刘茵.慢病毒载体在转基因动物研制中的应用[J].国际遗传学杂志(LIU Yin.Application of lentiviral vectors intransgenic animal development[J].Int J Genet),2007,30(5):374-377.[8]MAY C,RIVELLA S,CALLEGARI J,et al.Therapeutichaemoglobin synthesis in beta-thalassaemic mice expressinglentivirus-encoded human beta-globin[J].Nature,2000,406(6791):82-86.[9]HAN X D,LIN C,CHANG J,et al.Fetal gene therapy ofα-thalassemia in a mouse model[J].PNAS,2007,104(21):9007-9011.[10]LI W,XIE S Y,GUO X B,et al.A novel transgenic mousemodel produced from lentiviral germline integration for thestudy ofβ-thalassemia gene therapy[J].Haematologica,2008,93(3):357-362.[11]LOIS C,HONG E J,PEASE S,et al.Germline transmissionand tissuespecific expression of transgenes delivered bylentiviral vectors[J].Science,2002,295:868-872.[12]PFEIFER A,IKAWA M,DAYN Y,et al.Transgenesis bylentiviral vectors:lack of gene silencing in mammnlianembryonic stem cells and preimpalntation embryos[J].ProcNatl Acad Sci U S A,2002,99:2140-2145.[13]HOFMANN A,KESSLER B,EWERLING S,et al.Efficienttransgenesis in farm animals by lentiviral emtovora vectors[J].EMBO Reports,2003,4:1054-1060.[14]HOFMANN A,ZAKHARTCHENKO V,WEPPERT M,et al.Generation of transgenic cattle by lentiviral gene transfer intooocytes[J].Biol Reprod,2004,71:405-409.398。

a novel indexing approach for multimedia image databases

a novel indexing approach for multimedia image databases

INTRODUCTION
Huge volumes of image data accumulate and are stored for diverse applications. These include medical imagery, sattelite imagery, entertainment image data, etc. As manual search and retrieval of images in large databases is impractical, efficient automatic access techniques are needed. The problem is exacerbated by the need for content-based retrieval rather than the alphanumeric search typical of traditional database management. This is a major challenge that has been recognized by many researchers and developers [l,21. Several products are currently available on the internet, and offer good performance when queries are well captured by color indexing via histograms, etc. Nevertheless, the main difficulty is due t o the wide diversity of users and queries whose needs are not satisfied by such simple search techniques. The human notion of similarity is subjective and hard to define precisely. It depends on the type of database, the context, the application and the user. In this paper we propose to measure the similarity of two pictures, by applying local similarity principles from the fractal coding theory [ 3 ] . This approach to formalating similarity offers much flexibility, and the premise of this work is that it is a useful tool for approximating the subjective aspects of an image database query. In most fractal-based image indexing algorithms,

anovelapproachto...

anovelapproachto...

A novel approach to translymphaticchemotherapy targeting sentinel lymph nodes of patients with oral cancer using intra-arterial chemotherapy -preliminary studyJunkichi Yokoyama *,Shin Ito,Shinichi Ohba,Mitsuhisa Fujimaki and Katsuhisa IkedaIntroductionThe sentinel lymph node (SLN)is defined as the lymph node that firstly receives lymphatic drainage from the primary cancer [1].The SLN is thought to be the first possible micrometastatic site via lymphatic drainage from the primary cancer.Thus,the pathological status of the SLN can predict the status of all regional lymph nodes.If the SLN is recognized as being negative forcancer metastasis,unnecessary dissection may be avoided and a positive prognosis achieved.This SLN concept is well established in the treatment of patients with several types of solid carcinomas,such as mela-noma and breast cancer [2-4].The SLN concept has revolutionized the approach to surgical staging of both the melanoma and breast cancer,and these techniques can benefit patients by preventing various complications due to unnecessary prophylactic dissection when the SLN is negative for cancer metastasis.Recently,the SLN concept has been extended to many other solid tumors,*Correspondence:*******************.jpDepartment of Otolaryngology,Head and Neck Surgery,Juntendo University School of Medicine,Tokyo,JapanYokoyama et al .Head &Neck Oncology 2011,3:42/content/3/1/42©2011Yokoyama et al;licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),which permits unrestricted use,distribution,and reproduction in any medium,provided the original work is properly cited.including head and neck cancers[5,6].In this study,we consider a newly developed translymphatic chemother-apy procedure targeting the SLN using intra-arterial chemotherapy for oral cancer to improve prognosis and to preserve significant organs[7-9].ObjectiveEvaluate CDDP concentrations in SLNs and non-SLNs. Determine the usefulness of translymphatic chemother-apy targeting SLNs in patients with oral cancer using intra-arterial chemotherapy.Method and PatientsFive patients with tongue cancer(T3N0M0)were trea-ted by intra-arterial chemotherapy as neoadjuvant che-motherapy from November2010to June2011.After a week of chemotherapy,surgical treatment including par-tial resection of the tongue and neck dissection was per-formed.Intra-arterial chemotherapy was administeredat50mg/m2of CDDP either one or two times weekly. CT-angiography confirmed that the areas of tongue can-cer were stained and that lymph nodes were not stained (Figure1).Five mg of ICG was administered via a cathe-ter positioned in the lingual artery at the beginning of the surgery(Figure2).SNLs were detected by ICG fluorescence imaging(Photodynamic Eye,Hamamatsu Photonics)and non-SNLs were detected in two subman-dibular lymph nodes located near the tongue cancer. These were monitored as controls.In order to measure CDDP concentrations,0.1g of each of the SLNs and the two non-SLNs were resected and the rests of each of the SLNs were examined intraoperatively by means of routine frozen pathological examination.The CDDP concentrations were measured by atomic absorption analysis.A conventional method of identifying SLNs using radioactive injection was also performed the day before surgery.The pre-treatment characteristics of the patients are shown in table1.Patients’informed consent was obtained prior to treat-ment,and this study was approved by the Human Ethics Review Committee of Juntendo University.The difference between the two groups CDDP con-centrations were tested by Student’s t-test and Wil-coxon test.;p values<0.05were considered to indicate significance.ResultsDetection of SLNs were clearly demonstrated by ICG fluorescence imaging(Figure3,4).The mean number of SLNs was5.6(3-8).ICG fluorescence imaging showed a greater number of SLNs in our intra-arterial infusion than seen when injecting radiocolloid intratumor(mean 3.4).SLNs detected by ICG fluorescence imaging included all of the SLNs detected by the conventional radioactive method.Histopathological examination was performed for29 SLNs and90non-SLNs(Table1).All5patients with his-topathologically verified metastasis in their SLNs demon-strated positive results in ICG fluorescence imaging.No false negative cases were identified within each SLN basin. However,of the7metastatic lymph nodes,one was not identified by means of conventional methods.The mean CDDP concentrations of SLNs and non-SNLs were1.2μg/g and0.35μg/g respectively.TheFigure1CT-angiography infusing the lingual artery.CT-angiography confirmed the stained tongue cancer(a and b) indicated by triangls.There was no staining in any lymph nodes(c and d)indicated by arrowheads.Arrows represent the catheter inserted in the lingual artery.Figure2Tongue cancer after injection of ICG.a:tongue cancer, b:tongue cancer with ICG fluorescence imaging.CDDP concentration of SLNs was significantly higher than non-SLNs.The mean CDDP concentration of ton-gue cancer was2.3μg/g.No hematological complications were caused by intra-arterial chemotherapy.All patients are alive with no evi-dence of disease and are able to consume food as they were able to before surgery.DiscussionChemoradiation therapy has significantly enhanced the preservation of important organs in the treatment of head and neck cancer.However,because of severe mucositis and low sensitivity to chemotherapy,tongue cancer has not been treated by chemoradiation as often as other sites of head and neck cancer[10].CDDP is a most promising drug for the treatment of head and neck cancers.To increase the CDDP concentration in tongue cancer resistant to chemotherapy we have adopted intra-arterial chemotherapy for the treatment of advanced tongue cancer.This procedure has resulted in a positive prognosis and good organ preservation[7,9]. We found that the administration of CDDP to the pri-mary tongue cancer has a powerful effect on the pri-mary cancer as well as occult neck metastasis.As a result,we have hypothesized that intra-arterial chemotherapy for the treatment of primary tongue cancer,also results in translymphatic chemotherapy to control the subclinical metastatic tumor in SLNs.The schema of translymphatic chemotherapy is illustrated in Figure5.This schema shows that CDDP adminis-tered to the primary tongue cancer moves selectively to SLNs via lymphatic canals.CDDP is accumulated in the SNLs and results in a high CDDP concentration in the pared with the 2.3μg/g CDDPTable1Patients characteristicscases site age M/F TNM No of SLNs by radiocolloid No of SLNs by ICG No of non-SLNs 1tongue34M T3N0M034112tongue57F T3N0M03(FN)6213tongue37M T3N0M036144tongue63M T3N0M046165tongue59M T3N0M04728Mean50 3.4 5.618FN:False Negative,LN:Lymph nodeIntraoperative navigation surgery using ICG fluorescence imaging.Number(1~5)means SLNs.a and b represent represent level III and IV dissection.concentration measured within the tongue cancer,the mean CDDP concentration measured in SLNs was recorded at1.2μg/g.However,the difference between the CDDP concentrations of SLNs and tongue cancer was significant.In our preliminary study,all SLNs were detected by ICG fluorescence imaging infused via the lingual artery in5cT3N0tongue cancer patients.The number of SLNs resulting from intra-arterial infusion was greater than could be seen when by means of conventionalspecimens.a:Rt side represents the caudal side.Number(1~7)represents SLNs.b:level I,c:level II and III,injection to the intratumor.This is because ICG was administered to the lingual artery and ICG spread throughout half of the tongue(Figure2).ICG moved via lymphatic canals from half of the tongue including the tongue cancer.Even in micrometastatic SLNs,an affer-ent lymphatic sometimes occluded by micrometastatic cancer based on sentinel navigation or CT lymphograpy [11].In our examination,we also did not detect a meta-static SLN by conventional methods due to occlusion of afferent lymphatics from the tongue cancer(Figure6).It contained CDDP as high as1.68μg/g.This was because, each lymph node has several afferent lymphatics and ICG or CDDP could move to micrometastatic SLN via several other afferent lymphatics in the case of intra-arterial infusion.CDDP was released continuously from the primary tongue cancer via the translymphatic canal for a period of over more than one week.CDDP was selectively accumulated in SLNs and continued to effect micrometastasis in SLNs over a long period.After a per-iod of several weeks,the CDDP concentrations between the primary cancer and SLNs gradually will become the same and maintained equilibrium.Our intra-arterial chemotherapy is suspected to contribute not only to pri-mary organ preservation,but also to a positive prognosis by controlling the metastatic SLNs.Preservation of patients quality of life in advanced cT3N0tongue cancer is achieved by means of intra-arterial chemotherapy and through targeting SLN metastasis with translympha-tic chemotherapy.We believe that ICG fluorescence imaging is very useful for navigation surgery as there appear to be no limitations.An additional reason for difficulties in detecting SLNs was the close proximity of the primary tumor to the lymph node basin.This caused difficulties for both preoperative lymphoscintigraphy and intraoperative radi-olocalization,because of the well described phenomena of“shine-through’’radioactivity and scatter from the primary site[4].Specifically,it was particularly difficult to detect SLNs on the floor of the mouth in any other sites of head and neck cancers[12,13].In order to avoid the influence of‘shine-through’we firstly resected the close primary tumor before sentinel mapping.However, it was difficult to completely avoid the influence of ‘shine-through’after resection of the primary tumor.As for ICG fluorescence imaging,SLNs were clearly detected even in close proximity to the primary tumor and‘shine-through’could be avoided.The ICG fluores-cence imaging procedure demonstrated better success rates of detecting SLNs for patients with tumors in the floor of the mouth than the radioactivity method. Further studies will be required to verify the effective-ness and safety of intra-arterial chemotherapy as a method of lymphatic chemotherapy for the treatment of occult lymph node metastatsis.Our results suggest that a drug delivery system based on the SLN concept should be developed for local chemotherapy targeting SLNs in patients with cN0oral cancer,for whom there is poten-tial for metastasis in SLNs.Further investigations may lead to the development of a new minimally invasive multimodal therapy targeting both the primary tumor and SLNs in the near future. ConclusionOur study verified the possibility that intra-arterial che-motherapy may be effective not only for organ preserva-tion therapy,but also serve as an efficient procedure for translymphatic chemotherapy targeting SLNs in patients with oral cancer through the use of ICG fluorescence imaging.The CDDP concentrations recorded in SLNs were significantly higher than in non-SNLs.This novel drug delivery system is feasible for trans-lymphatic chemotherapy targeting SLNs in patients with cT3N0oral cancer with the possibility of occult metas-tasis in SLNs.AcknowledgementsThis research was funded in part by a Grant for Clinical Cancer Research from the Ministry of Health,Labor,and Welfare of Japan.Authors’contributionsJY and SI prepared and edited this manuscript.SO and MF contributed to the collection of data.KI performed the statistical analysis.JY and KI gave final approval for this version of the manuscript.All authors read and approved the final manuscriptCompeting interestsThe authors declare that they have no competing interests.Received:2August2011Accepted:19September2011Published:19September 2011Figure6A metastatic SLN not detected by the conventional method.a:left side low power magnification.b:right side high power magnification.This lymph node contained CDDP as high as 1.68μg/g.References1.Morton DL,Wen DR,Wong JH,Economou JS,Cagle LA,Storm FK,Foshag LJ,Cochran AJ:Technical details of intraoperative lymphaticmapping for early stage melanoma.Arch Surg1992,127:392-399.2.Giuliano AE,Kirgan DM,Guenther JM,Morton DL:Lymphatic mapping andsentinel Lymphadene-ctomy for breast cancer.Ann Surg1994,220:391-401.3.Morton DL,Thompson JF,Essner R,Elashoff R,Stern SL,Nieweg OE,Roses DF,Karakousis CP,Mozzillo N,Reintgen D,Wang HJ,Glass EC,Cochran AJ:Validation of the accuracy of intraoperative lymphaticmapping and sentinel lymphadenectomy for early-stage melanoma:amulticenter trial.Multicenter Selective Lymphadenectomy Trial Group.Ann Surg1999,230:453-463.4.Krag D,Weaver D,Ashikaga T,Moffat F,Klimberg VS,Shriver C,Feldman S,Kusminsky R,Gadd M,Kuhn J,Harlow S,Beitsch P:The sentinel node inbreast cancer-a multicenter validation study.N Engl J Med1998,339:941-946.5.Rinaldo A,Devaney KO,Ferlito A:Immunohistochemical studies in theidentification of lymph node micrometastases in patients withsquamous cell carcinoma of the head and neck.ORL J Otorhinolaryngol Relat Spec2004,66:38-41.6.De Cicco C,TrifiròG,Calabrese L,Bruschini R,Ferrari ME,Travaini LL,Fiorenza M,Viale G,Chiesa F,Paganelli G:Lymphatic mapping to tailorselective lymphadenectomy in cN0tongue carcinoma:beyond thesentinel node concept.Eur J Nucl Med Mol Imaging2006,33:900-5.7.Yokoyama Junkichi:Present role and future prospect of superselectiveintra-arterial infusion chemotherapy for head and neck cancer.Jpn JChemother2002,29:169-175.8.Shiga Kiyoto,Yokoyama Junkichi,Hashimoto Sho,Saijo S,Tateda M,Ogawa T,Watanabe M,Kobayashi T:Combined therapy aftersuperselective arterial cisplatin infusion to treat maxillary squamous cell carcinoma.Otolaryngol Head and Neck Surg2007,136:1003-1009.9.Robbins KT:The evolving role of combined modality therapy in headand neck cancer.Arch Otolaryngol Head Neck Surg2000,126:265-269. 10.Hanna E,Alexiou M,Morgan J,Badley J,Maddox AM,Penagaricano J,Fan CY,Breau R,Suen J:Intensive chemoradiotherapy as a primarytreatment for organ preservation in patients with advanced cancer ofthe head and neck:efficacy,toxic effects,and limitations.ArchOtolaryngol Head Neck Surg2004,130:861-7.11.Matsuzuka T,Kano M,Ogawa H,Miura T,Tada Y,Matsui T,Yokoyma S,Suzuki Y,Suzuki M,Omori K:Sentinel node mapping for node positiveoral cancer:potential to predict multiple ryngoscope2008, 118:646-9.12.Civantos F,Zitsch R,Bared A:Sentinel node biopsy in oral squamous cellcarcinoma.J Surg Oncol2007,96:330-6.13.Ross GL,Soutar DS,MacDonald DG,Shoaib T,Camilleri I,Roberton AG,Sorensen JA,Thomsen J,Grupe P,Alvarez J,Barbier L,Santamaria J,Poli T, Massarelli O,Sesenna E,Kovács AF,Grünwald F,Barzan L,Sulfaro S,Alberti F:Sentinel node biopsy in head and neck cancer:preliminaryresults of a multicenter trial.Ann Surg Oncol2004,11:690-6.。

intraprediction,HEVC里面的帧内编码详解

intraprediction,HEVC里面的帧内编码详解

Intra Coding of the HEVC StandardJani Lainema,Frank Bossen,Member,IEEE,Woo-Jin Han,Member,IEEE,Junghye Min,and Kemal UgurAbstract—This paper provides an overview of the intra coding techniques in the High Efficiency Video Coding(HEVC)standard being developed by the Joint Collaborative Team on Video Coding (JCT-VC).The intra coding framework of HEVC follows that of traditional hybrid codecs and is built on spatial sample prediction followed by transform coding and postprocessing steps.Novel features contributing to the increased compression efficiency include a quadtree-based variable block size coding structure, block-size agnostic angular and planar prediction,adaptive pre-and postfiltering,and prediction direction-based transform coefficient scanning.This paper discusses the design principles applied during the development of the new intra coding methods and analyzes the compression performance of the individual tools. Computational complexity of the introduced intra prediction algorithms is analyzed both by deriving operational cycle counts and benchmarking an optimized ing objective metrics,the bitrate reduction provided by the HEVC intra coding over the H.264/advanced video coding reference is reported to be 22%on average and up to36%.Significant subjective picture quality improvements are also reported when comparing the resulting pictures atfixed bitrate.Index Terms—High Efficiency Video Coding(HEVC),image coding,intra prediction,Joint Collaborative Team on Video Coding(JCT-VC),video coding.I.IntroductionT HE TWO prominent international organizations specify-ing video coding standards,namely ITU-T Video Coding Experts Group(VCEG)and ISO/IEC Moving Picture Experts Group(MPEG),formed the Joint Collaborative Team on Video Coding(JCT-VC)in April2010.Since then,JCT-VC has been working toward defining a next-generation video coding standard called High Efficiency Video Coding(HEVC).There are two major goals for this standard.First,it is targeted to achieve significant improvements in coding efficiency com-pared to H.264/Advanced Video Coding(A VC)[1],especially when operating on high-resolution video content.Second,the standard should have low enough complexity to enable high-resolution high-quality video applications in various use cases, Manuscript received April13,2012;revised July18,2012;accepted August 21,2012.Date of publication October2,2012;date of current version January 8,2013.This work was supported by Gachon University under the Research Fund of2012GCU-2011-R288.This paper was recommended by Associate Editor O.C.Au.(Corresponding author:W.-J.Han.)inema and K.Ugur are with the Nokia Research Center,Tampere 33720,Finland(e-mail:inema@;kemal.ugur@).F.Bossen is with DOCOMO Innovations,Inc.,Palo Alto,CA94304USA (e-mail:bossen@).W.-J.Han is with Gachon University,Seongnam461-701,Korea(e-mail: hurumi@).J.Min is with Samsung Electronics,Suwon442-742,Korea(e-mail: jh643.min@).Color versions of one or more of thefigures in this paper are available online at .Digital Object Identifier10.1109/TCSVT.2012.2221525including operation in mobile environments with tablets and mobile phones.This paper analyzes different aspects of the HEVC intra coding process and discusses the motivation leading to the selected design.The analysis includes assessing coding efficiency of the individual tools contributing to the overall performance as well as studying complexity of the introduced new algorithms in detail.This paper also describes the methods HEVC utilizes to indicate the selected intra coding modes and further explains how the tools are integrated with the HEVC coding block architecture.Intra coding in HEVC can be considered as an extension of H.264/A VC,as both approaches are based on spatial sample prediction followed by transform coding.The basic elements in the HEVC intra coding design include:1)quadtree-based coding structure following the HEVCblock coding architecture;2)angular prediction with33prediction directions;3)planar prediction to generate smooth sample surfaces;4)adaptive smoothing of the reference samples;5)filtering of the prediction block boundary samples;6)prediction mode-dependent residual transform and coef-ficient scanning;7)intra mode coding based on contextual information.In addition,the HEVC intra coding process shares several processing steps with HEVC inter coding.This processing, including,e.g.,transformation,quantization,entropy coding, reduction of blocking effects,and applying sample adaptive offsets(SAO),is outside of the scope of this paper.This paper is organized as follows.Section II explains the motivation leading to the selected design for HEVC intra coding.It also describes the overall HEVC coding architecture and the new intra prediction tools introduced in the HEVC standard.Section III presents the intra mode coding and the residual coding approaches of the standard.Section IV provides complexity analysis of the introduced intra prediction processes with detailed information about the contribution of different intra prediction modes.Section V summarizes the compression efficiency gains provided by the proposed design and Section VI concludes this paper.II.HEVC Intra Coding ArchitectureIn H.264/A VC,intra coding is based on spatial extrapolation of samples from previously decoded image blocks,followed by discrete cosine transform(DCT)-based transform coding [2].HEVC utilizes the same principle,but further extends it to be able to efficiently represent wider range of textural and1051-8215/$31.00c 2012IEEEstructural information in images.The following aspects were considered during the course of HEVC project leading to the selected intra coding design.1)Range of supported coding block sizes:H.264/A VCsupports intra coded blocks up to the size of16×16 pixels.This represents a very small area in a high-definition picture and is not large enough to efficiently represent certain textures.2)Prediction of directional structures:H.264/A VC sup-ports up to eight directional intra prediction modes fora given block.This number is not adequate to predictaccurately directional structures present in typical video and image content,especially when larger block sizes are used.3)Prediction of homogeneous regions:The plane mode ofH.264/A VC was designed to code homogeneous imageregions.However,this mode does not guarantee con-tinuity at block boundaries,which can create visible artifacts.Thus,a mode that guarantees continuous pre-diction sample surfaces would be desired.4)Consistency across block sizes:H.264/A VC uses differ-ent methods to predict a block depending on the size of the block and the color component the block represents.A more consistent design is preferred,especially asHEVC supports a large variety of block sizes.5)Transforms for intra coding:H.264/A VC utilizes afixedDCT transform for a given block size.This design does not take into consideration different statistics of the pre-diction error along the horizontal and vertical directions depending on the directionality of the prediction.6)Intra mode coding:Due to the substantially increasednumber of intra modes,more efficient coding techniques are required for mode coding.The coding structure utilized for intra coding in HEVC follows closely the overall architecture of the codec.Images are split into segments called coding units(CU),predic-tion units(PU),and transform units(TU).CU represent quadtree split regions that are used to separate the intra and inter coded blocks.Inside a CU,multiple nonoverlapping PU can be defined,each of which specifies a region with individual prediction parameters.CU are further split into a quadtree of transform units,each TU having a possibility of applying residual coding with a transform of the size of the TU.HEVC contains several elements improving the efficiency of intra prediction over earlier solutions.The introduced methods can model accurately different directional structures as well as smooth regions with gradually changing sample values. There is also emphasis on avoiding introduction of artificial edges with potential blocking effects.This is achieved by adaptive smoothing of the reference samples and smoothing the generated prediction boundary samples for DC and directly horizontal and vertical modes.All the prediction modes utilize the same basic set of reference samples from above and to the left of the image block to be predicted.In the following sections,we denote the reference samples by R x,y with(x,y)having its origin one pixel above and to the left of the block’s top-leftcorner.Fig.1.Reference samples R x,y used in prediction to obtain predicted samples P x,y for a block of size N×N samples.TABLE ISpecification of Intra Prediction Modes and Associated Names Intra Prediction Mode Associated Names0Planar1DC2..34Angular(N),N=2 (34)Similarly,P x,y is used to denote a predicted sample value at a position(x,y).Fig.1illustrates the notation used. Neighboring reference samples may be unavailable for intra prediction,for example,at picture or slice boundaries,or at CU boundaries when constrained intra prediction is enabled. Missing reference samples on the left boundary are generated by repetition from the closest available reference samples below(or from above if no samples below are available). Similarly,the missing reference samples on the top boundary are obtained by copying the closest available reference sample from the left.If no reference sample is available for intra prediction,all the samples are assigned a nominal average sample value for a given bit depth(e.g.,128for8-b data). It should be noted that in addition to left,above,and above-right reconstructed samples used for H.264/A VC intra prediction,below-left side of samples(R0,N+1..R0,2N)are also used for HEVC.These samples were excluded from the H.264/A VC process as they were rarely available in the traditional macroblock-based coding structure[2],but the hierarchical HEVC coding architecture makes these candidates available more frequently.HEVC design supports a total of35intra prediction modes. Table I specifies the numbers and names associated with each mode.In this paper,intra prediction mode0refers to the planar intra prediction,mode1to DC prediction,and modes2to 34to angular prediction modes with different directionalities. Fig.2further illustrates the prediction directions associated with the angular modes.A.Angular Intra PredictionAngular intra prediction in HEVC is designed to be able to efficiently model different directional structures typicallyFig.2.HEVC angular intra prediction modes numbered from2to34and the associated displacement parameters.H and V are used to indicate the horizontal and vertical directionalities,respectively,while the numeric part of the identifier refers to the pixels’displacement as1/32pixel fractions. present in video and image contents.The number and angu-larity of prediction directions are selected to provide a good tradeoff between encoding complexity and coding efficiency for typical video material.The sample prediction process itself is designed to have low computational requirements and to be consistent across block sizes and prediction directions.The latter aims at minimizing the silicon area in hardware and the amount of code in software implementations.It is also intended to make it easier to optimize the implementation for high performance and throughput in various environments. This is especially important as the number of block sizes and prediction directions supported by HEVC intra coding far exceeds those of previous video codecs,such as H.264/A VC. In HEVC,there are four effective intra prediction block sizes ranging from4×4to32×32samples,each of which supports 33distinct prediction directions.A decoder must thus support 132combinations of block size and prediction direction. The following sections discuss in further detail different aspects contributing to the coding performance and implemen-tation complexity of the HEVC angular intra prediction.1)Angle Definitions:In natural imagery,horizontal and vertical patterns typically occur more frequently than patterns with other directionalities.The set of33prediction angles is defined to optimize the prediction accuracy based on this observation[7].Eight angles are defined for each octant with associated displacement parameters,as shown in Fig.2. Small displacement parameters for modes close to horizon-tal and vertical directions provide more accurate prediction for nearly horizontal and vertical patterns.The displacement parameter differences become larger when getting closer to diagonal directions to reduce the density of prediction modes for less frequently occurringpatterns.Fig.3.Example of projecting left reference samples to extend the top reference row.The bold arrow represents the prediction direction and the thin arrows the reference sample projections in the case of intra mode23 (vertical prediction with a displacement of−9/32pixels per row).2)Reference Pixel Handling:The intra sample prediction process in HEVC is performed by extrapolating sample values from the reconstructed reference samples utilizing a given directionality.In order to simplify the process,all sample locations within one prediction block are projected to a single reference row or column depending on the directionality of the selected prediction mode(utilizing the left reference column for angular modes2to17and the above reference row for angular modes18to34).In some cases,the projected pixel locations would have negative indexes.In these cases,the reference row or column is extended by projecting the left reference column to extend the top reference row toward left,or projecting the top reference row to extend the left reference column upward in the case of vertical and horizontal predictions,respectively.This approach was found to have a negligible effect on compression performance,and has lower complexity than an alternative approach of utilizing both top and left references selectively during the prediction sample generation process[5].Fig.3 depicts the process for extending the top reference row with samples from the left reference columns for an8×8block of pixels.3)Sample Prediction for Arbitrary Number of Directions: Each predicted sample P x,y is obtained by projecting its location to a reference row of pixels applying the selected prediction direction and interpolating a value for the sample at1/32pixel accuracy.Interpolation is performed linearly utilizing the two closest reference samplesP x,y=32−w y·R i,0+w y·R i+1,0+16>>5(1)where w y is the weighting between the two reference samples corresponding to the projected subpixel location in between R i,0and R i+1,0,and>>denotes a bit shift operation to the right.Reference sample index i and weighting parameter w y are calculated based on the projection displacement d associated with the selected prediction direction(describing the tangent of the prediction direction in units of1/32samplesand having a value from −32to +32as shown in Fig.2)asc y =(y ·d )>>5w y =(y ·d )&31i =x +c y(2)where &denotes a bitwise AND operation.It should be noted that parameters c y and w y depend only on the coordinate y and the selected prediction displacement d .Thus,both parameters remain constant when calculating predictions for one line of samples and only (1)needs to be evaluated per sample basis.When the projection points to integer samples (i.e.,when w y equals zero),the process is even simpler and consists of only copying integer reference samples from the reference row.Equations (1)and (2)define how the predicted sample values are obtained in the case of vertical prediction (modes 18to 34)when the reference row above the block is used to derive the prediction.Prediction from the left reference column (modes 2to 17)is derived identically by swapping the x and y coordinates in (1)and (2).B.Planar PredictionWhile providing good prediction in the presence of edges is important,not all image content fits an edge model.The DC prediction provides an alternative but is only a coarse approximation as the prediction is of order 0.H.264/A VC features an order-1plane prediction mode that derives a bilinear model for a block using the reference samples and generates the prediction using this model.One disadvantage of this method is that it may introduce discontinuities along the block boundaries.The planar prediction mode defined in HEVC aims to replicate the benefits of the plane mode while preserving continuities along block boundaries.It is essentially defined as an average of two linear predictions (see [8,Fig.2]for a graphical representation)P V x,y =(N −y )·R x,0+y ·R 0,N +1P H x,y =(N −x )·R 0,y +x ·R N +1,0P x,y = P Vx,y +P Hx,y +N >> log 2(N )+1 .(3)C.Reference Sample SmoothingH.264/A VC applies a three-tap smoothing filter to the ref-erence samples when predicting 8×8luma blocks.HEVC uses the same smoothing filter ([121]/4)for blocks of size 8×8and larger.The filtering operation is applied for each reference sample using neighboring reference samples.The first reference sample R 0,2N and R 2N,0are not filtered.For 32×32blocks,all angular modes except horizontal and vertical use a filtered reference.In 16×16blocks,the modes not using a filtered reference are extended to the four modes (9,11,25,27)closest to horizontal and vertical.Smoothing is also applied where the planar mode is used,for block sizes 8×8and larger.However,HEVC is more discerning in the use of this smoothing filter for smaller blocks.For 8×8blocks,only the diagonal modes (2,18,34)use a filtered reference.Applying the reference sample smoothing selectively based on the block size and directionality of the prediction is reported to reduce contouring artifacts caused by edges in the reference sample arrays [23].D.Boundary SmoothingAs noted above in the case of the H.264/A VC plane predic-tion mode,DC and angular prediction modes may introduce discontinuities along block boundaries.To remedy this prob-lem,the first prediction row and column are filtered in the case of DC prediction with a two-tap finite impulse response filter (corner sample with a three-tap filter).Similarly,the first prediction column for directly vertical prediction and the first prediction row for directly horizontal prediction are filtered utilizing a gradient-based smoothing [9].For a more complete description of the filtering process,please refer to [25].As the prediction for chroma components tends to be very smooth,the benefits of the boundary smoothing would be limited.Thus,in order to avoid extra processing with marginal quality improvements,the prediction boundary smoothing is only applied to luma component.The average coding effi-ciency improvement provided by the boundary smoothing is measured to be 0.4%in HM 6.0environment [14].E.I−PCM Mode and Transform Skipping ModeHEVC supports two special coding modes for intra coding denoted as I −PCM and transform skipping mode.In I −PCM mode,prediction,transform,quantization,and entropy coding are bypassed while the prediction samples are coded by a predefined number of bits.The main purpose of the I −PCM mode is to handle the situation when the signal cannot be efficiently coded by other modes.On the contrary,only transform is bypassed in transform skipping mode.It was adopted to improve the coding effi-ciency for specific video contents such as computer-generated graphics.HEVC restricts the use of this mode when the block size is equal to 4×4to avoid the significant design change due to this mode while this choice was proven to have most of coding efficiency benefits.F .Restrictions for Partitioning TypesHEVC intra coding supports two types of PU division,PART −2N ×2N and PART −N ×N ,splitting a CU into one or four equal-size PUs,respectively.However,the four regions specified by the partitioning type PART −N ×N can be also represented by four smaller CU with the partitioning type PART −2N ×2N .Due to this,HEVC allows an intra CU to be split into four PU only at the minimum CU size.It has been demonstrated that this restriction is associated with minimal impact in coding efficiency,but it reduces the encoding complexity significantly [3].Another way to achieve the same purpose would be reducing the CU size to 4×4with the partitioning mode PART −2N ×2N .However,this approach would result in a chroma intra prediction block size of 2×2pixels,which can be critical to the throughput of the entire intra prediction process.For this reason,the minimum allowed CU size is restricted to 8×8pixels while allowing the partitioning type PART −N ×N only in the smallest CU.G.TU-Based PredictionWhen a CU is split into multiple TU,the intra prediction is applied for each TU sequentially instead of applying theintra prediction at the PU level.One obvious advantage for this approach is the possibility of always utilizing the nearest neighboring reference samples from the already reconstructed TU.It has been shown that this property improves the intra picture coding efficiency by about1.2%[4]compared to the case of using PU border reference samples for the PU.It should be noted that all the intra prediction information is indicated per PU and the TUs inside the same PU share the same intra prediction mode.III.Mode and Residual CodingA.Mode Coding for LumaHEVC supports total33angular prediction modes as well as planar and DC prediction for luma intra prediction for all the PU sizes.Due to the large number of intra prediction modes, H.264/A VC-like mode coding approach based on a single most probable mode was not found effective in HEVC[8].Instead, HEVC defines three most probable modes for each PU based on the modes of the neighboring PUs.The selected number of most probable modes makes it also possible to indicate one of the32remaining modes by afixed length code,as the distribution of the mode probabilities outside of the set of most probable modes is found to be relatively uniform.The selection of the set of three most probable modes is based on modes of two neighboring PUs,one above and one to the left of the current PU.By default,modes of both candidate PUs are included in the set.Possible duplicates and the third mode in the set are assigned with modes planar,DC,or angular (26)in this order.In the case that both top and left PU has the same directional mode,that mode and two closest directional modes are used to construct the set of most probable modes instead.In the case current intra prediction mode is equal to one of the elements in the set of most probable modes,only the index in the set is transmitted to the decoder.Otherwise,a5-bfixed length code is used to determine the selected mode outside of the set of most probable modes.B.Derived Mode for Chroma Intra PredictionQuite often structures in the chroma signal follow those of the luma.Taking advantage of this behavior,HEVC introduces a mechanism to indicate the cases when chroma PU utilizes the same prediction mode as the corresponding luma PU.Table II specifies the mode arrangement used in signaling the chroma mode.In the case that derived mode is indicated for a PU,the prediction is performed by using the corresponding luma PU mode.In order to remove the possible redundancy in the signaling arising when derived refers to one of the modes always present,angular(34)mode is used to substitute the duplicate mode as shown in Table II.C.Syntax Design for the Intra Mode CodingHEVC uses three syntax elements to represent the luma intra prediction mode.The syntax element prev−intra−luma−pred−flag specifies whether the luma intraTABLE IISpecification of Chroma Intra PredictionModes and Associated NamesChroma intra Alternative mode,ifprediction Primary mode primary mode is equal tomode the derived mode0Planar Angular(34)1Angular(26)Angular(34)2Angular(10)Angular(34)3DC Angular(34)4Derived N/A prediction mode is matched with one of three most probable modes.When theflag is equal to1,the syntax element mpm−idx is parsed,indicating which element of the most probable mode array specifies the luma intra prediction mode.When theflag is equal to0,the syntax element rem−intra−luma−pred−mode is parsed,specifying the luma intra prediction mode as rem−intra−luma−pred−mode+N, where N is the number of elements in the most probable mode array having a mode index greater than or equal to the syntax element rem−intra−luma−pred−mode.In chroma mode coding,a1-b syntax element(0)is assigned to the most often occurring derived mode,while3-b syntax elements(100,101,110,111)are assigned to the remaining four modes.D.Intra Mode Residual CodingHEVC utilizes intra mode dependent transforms and co-efficient scanning for coding the residual information.The following sections describe the selected approaches in more detail.1)Block Size-Dependent Transform Selection:Integer trans-forms derived from DCT and discrete sine transform(DST)are applied to the intra residual blocks.A DST-based transform is selected for4×4luma blocks and DCT-based transforms for the rest.For more details on the specification of the transforms, the reader is referred to[10]and[11].Different approaches to select the transforms were studied during the course of the HEVC development.For example, an early HEVC draft utilized a method that selected the horizontal and vertical transforms separately for4×4luma blocks based on the intra prediction mode.This approach provided an average coding efficiency improvement of0.8% [10].However,it was found that the simple block size de-pended approach now adopted in the standard provides very similar coding efficiency[26].Also larger size alternative transforms were studied,but it was reported that the additional coding efficiency improvements were marginal compared to the complexity impact[27].Thus,only DCT-based transforms are utilized for block sizes larger than4×4.2)Intra Prediction Mode-Dependent Coefficient Scanning: HEVC applies adaptive scanning of transform coefficients to the transform block sizes of4×4and8×8in order to take advantage of the statistical distribution of the active coefficients in2-D transform blocks.The scan is selected based on directionality of the intra prediction mode as shownTABLE IIIMapping Table Between Intra Prediction Modes and Coefficient Scanning OrderIntra Coefficient Coefficientprediction modescanning for scanning for4×4and8×816×16and32×32Angular(6–14)Vertical DiagonalAngular(22–30)Horizontal DiagonalAll other modes Diagonal Diagonalin Table III.Vertical and horizontal scans refer to the cor-responding raster scan orders,while the diagonal scan refersto a diagonal scan from down-left to up-right direction.Theaverage coding gain from the intra mode dependent scans isreportedly around1.2%[12].plexity of Intra Prediction Toolsplexity Analysis of Decoding Process1)Analysis of Operational Cycle Counts:Defining a propermetric for theoretical analysis of complexity can be difficultas various aspects come into play.For example,if relying oncounting operations,defining what constitutes one operationlargely depends on the underlying architecture.The bit widthof each data element being processed also plays an importantrole in determining a number of gates in hardware andthe amount of parallelism achievable in software.How analgorithm is implemented can also have a significant impact.A lower number of operations is not always better.Executingfour operations in parallel may be faster and considered lesscomplex than executing three in sequence.In this section,wethus limit ourselves to a more superficial comparison of HEVCprediction modes with the H.264/A VC ones.The DC,directly horizontal,and directly vertical predic-tion modes are the ones most similar to those defined inH.264/A VC.HEVC simply defines an additional postfilteringoperation where one row and/or column of samples isfiltered.The overhead of suchfiltering becomes less with larger blocksizes,as the fraction offiltered samples becomes smaller.For predicting a block of size N×N,angular predictionrequires the computation of p=(u·a+v·b+16)>>5foreach sample,which involves two multiplications(8-b unsignedoperands,16-b result),two16-b additions,and one16-b shiftper predicted sample,for a total offive operations.In theH.264/A VC case,directional prediction may take the formp=(a+2b+c+2)>>2,which may be considered to be five operations as well.However,on some architectures,thiscan be implemented using two8-b halving add operations:d=(a+c)>>1and p=(d+b+1)>> 1.The H.264/A VC directional prediction process is thus less complex as it requires no multiplication and no intermediate value wider than9b.In the case of the plane and planar modes,consideringthe generating equations is probably not adequate,as theprediction values can be efficiently computed incrementally.For the H.264/A VC plane mode,it is expected that one16-baddition,one16-b shift,and one clip to8-b range are requiredTABLE IVDecoding Time Comparison Between HM6.0Decoder and Its Optimized Decoder Used for Further AnalysisSettingBitrate HM6.0Optimized(Mbit/s)(s)(s) BasketballDrive,QP=2271.1104.4721.97BasketballDrive,QP=378.461.60 6.98BQTerrace,QP=22179.2163.9441.42BQTerrace,QP=3721.691.0012.03Cactus,QP=22105.7120.4628.84Cactus,QP=3714.269.598.78Kimono,QP=2222.337.117.02Kimono,QP=37 3.826.94 2.88ParkScene,QP=2252.760.5314.02ParkScene,QP=377.333.90 4.35Geometric mean25.466.3410.91Sequences are each10-s long and are coded in intra-only mode.Optimized decoder is about four to nine times faster than HM.per sample.For the HEVC planar mode,three16-b additions and one16-b shift are expected per sample.The partial analysis provided here is indicative.It suggests that the HEVC intra prediction modes are more complex than the A VC modes,but not necessarily by a large amount.In the following,focus is shifted to complexity investigation based on actual implementations.2)Decoding Time of Intra Prediction in Relation to Overall Decoding Time:In afirst experiment,the percentage of de-coding time spent on intra prediction is measured.Reference encodings for the main profile of HM6.0[14]are used in the all-intra configuration[15].The experiment is focused on high-definition sequences from the JCT-VC class B test set(five 1920×1080sequences).MD5checksum computation and file output of the reconstructed pictures are disabled such that decoding times more accurately reflect time spent on actually decoding pictures.Measurements are obtained on a laptop featuring an Intel Core i5processor clocked at 2.53GHz. Software was compiled using gcc4.6.3.In addition to the HM software,an optimized decoder,based on[16],is also considered.Table IV summarizes decoding times and gives an indication of the level of optimization.For the comparative results between HEVC HM decoder and H.264/A VC JM decoder,please refer to[24],which reported that they are comparable.The fraction of decoding time used for intra prediction is further summarized in Table V.While the results obtained with HM and optimized software show some differences,they do not diverge significantly:on average roughly12%–15%of decoding time is consumed by intra prediction.The results may however be somewhat surprising in the sense that generating the reference arrays used for prediction takes more time than generating the prediction from those reference arrays.Part of this observation may be caused by the fact that in both of the tested software implemen-tations,full reference arrays are constructed,including2N+1 samples for a row above and2N+1samples for a left column. Most prediction modes do not require all these samples,and constructing only the required ones may reduce the amount of time by up to50%.。

A novel technique

A novel technique

the short version daily. For scale developrnem and item reduction factor analyses were performed. Psychometric analyses included internal consistency, test-retest reliability, and responsiveness (calculated by the method of Cohen J, 1998). Construct validity was evaluated by comparison with the Gastrointestinal Symptom Rating Scale (GSRS) and the Psychological General Well Being (PGWB) index scale (Pearson and Spearman correlation coefficients). Results: On the basis of factor analyses ReQuest (long) was reduced by 7 items leading to a 60 items scale. Validation of ReQuest indicated very, high internal consistency reliability (Cronbach's alpha: 0.903), and tesbretest reliabflity (intradass<orrelation-coefficient: 0.94 [longqongl and 0.86 [short-short]). Responsiveness was fimnd to be large with a responsiveness index > 0.8 (1653). Construct validity was good (GSRS: Pearson r = 0.48, Spearman r = 0.52, PGWB: Pearson r = 0.48, Spearman r = 0.49). Conclusions: The new dimensionorientated symptom scale ReQuest is highly reproducible, valid and responsive. It provides a new valuable and highly sensitive, self-administered tool for evaluating treatment response in patients with erosive GERD (Supported by ALTANA Pharma AG).

Evaluation of a Novel Approach for Automated Inven

Evaluation of a Novel Approach for Automated Inven

Journal of Civil Engineering and Architecture 12 (2018) 550-562doi: 10.17265/1934-7359/2018.08.002Evaluation of a Novel Approach for Automated Inventive Conceptual Design of Building StructuresIvan RenevSchool of Engineering Science, Lappeenranta University of Technology (LUT), Lappeenranta 53850, FinlandAbstract: This research is dedicated to assessment of a method that was earlier proposed and developed in order to increase the degree of automation and software involvement into conceptual decision making during design of structural elements of buildings. Such instruments of the theory of inventive problem solving as contradiction and function analysis and trimming formed the basis of the proposed approach that was realized in a modern building information modeling software. The common logic of the approach is also provided in the article. Qualitative research methods and particularly collecting, analyzing and interpreting data were applied in this research. Firstly, a literature review of indexed journal articles in the field of the study was performed and some trends for possible development of the topic were identified. Secondly, a survey of potential users of the methodology was conducted and analyzed. The questionnaire results showed that the suggested method and its technical realization gained attraction among respondents, however, some of them are rather cautious regarding application of the approach potentials in their practice. The paper ends with evaluation results discussion, conclusion and proposals for further research.Key words: Buildings, structures, conceptual design, automation, TRIZ (theory of inventive problem solving), function analysis.1. IntroductionConceptual design is a fundamental part of a project life cycle and construction stage, of course, is also not an exception. Bearing buildings’ structures are clearly indispensable parts of buildings and should require special attention during design. The whole life cycle of a building, including its construction time and further operation, will be affected depending on how much competent and innovative solutions were taken at the earliest stage of design. A modern design engineer has access to unique design technologies and functional software. For instance, the BIM (building information modeling) is a process involving the generation and management of digital representations of physical and functional characteristics of buildings and the Autodesk Revit© that is one of the leading BIM software. Unfortunately, this is not always enough, especially for developing the buildingCorresponding author: Ivan Renev, doctoral student, research fields: building structures, conceptual design automation. concepts when it is required to take unique solutions in order to achieve the best results. In this case, existing software does not provide adequate support, since computer is only a thoughtless instrument in engineer’s hands during the conceptual design, however, it has a big potential to become a generator of new engineering ideas and proposals. That is why it is still relevant to suggest a technical approach that would increase the degree of automation and involvement of the software into conceptual decision making during design of buildings’ structures.This paper is a part of a project that is aimed at developing of a new methodology for automation of the conceptual design stage of buildings’ structural systems. Such methodology assumes an analysis of the buildings’ structural systems for its optimality and novelty and subsequent improvement of these indicators. During the work on the project existing techniques have been already discussed and analyzed that may be integrated into modern design software in order to enhance the level of design automation anddesign creativity. After studying different approachesEvaluation of a Novel Approach for Automated Inventive Conceptual Design of Building Structures 551of ideas generation it has been found that the TRIZ (theory of inventive problem solving) is well applicable for such purposes. TRIZ was created and developed in the former USSR by an engineer Genrikh Saulovich Altshuller. While working as a clerk in a patent office, he has analyzed a large number of patents and formulated some generic rules that would lead inventors to systematic creation of new inventive ideas [1]. Literature analysis on the topic has shown that TRIZ was successfully used for non-trivial systematic problem solving in construction and may also be a well applicable tool for achieving this project’s goals [2]. As a result, an approach was suggested for the early design stage automation in building projects where a number of TRIZ tools were integrated into a building information modeling software using an open source graphical programming tools [3, 4]. Such instruments of TRIZ as contradiction analysis, function analysis and trimming formed the basis of the proposed approach. The key goal of this particular study is evaluation of the proposed approach for automated inventive conceptual design of building structures.2. Research MethodsQualitative research methods that are particularly collecting, analyzing and interpreting data were applied in this research for evaluation of a novel approach for automated inventive conceptual design of building structures. This study has a number of key objectives that were, first, formulated in the form of the following RQs (research questions): RQ1—What is the current situation in the field of automation of conceptual design process in the construction projects? RQ2—What is the relevance of suggested approach? RQ3—What are possible advantages, disadvantages and to what extent it is easy to use the suggested algorithm? In order to answer these RQs, this paper is, firstly, aimed at systematization, analysis and understanding the current situation in the field of automation of conceptual design process in the construction field. Secondly, it focuses on testing the developed approach and its technical realization for understanding all the positive and negative signs and the convenience of its practical application.For answering RQ1 a systematic review of related literature was performed. RQ2 is answered by both analysis of existing similar proposals and collecting professional opinions using a specially developed questionnaire. For answering the RQ3 a set of questions was added into the developed questionnaire focusing on demonstration of the algorithm abilities.3. Literature ReviewThe literature review was based on journal articles indexed by the Scopus database as it is the largest abstract and citation database of peer-reviewed literature. It was decided to analyze the journal publications because their review and acceptance process is stricter comparing to conference proceedings and these kinds of scientific works are more comprehensive and self-contained.3.1 Creating the DatasetIn order to create the dataset of articles in the field of automation of early design stage in construction the following coherent search was performed: (1) the query “construction” and “design” and “automation” in Title or Abstract or Keywords. The search resulted to 3,600 papers. (2) Since those results came from different fields and included different types of documents, the subject area was limited to “engineering” and document type to “article”. Such limitation identified 1,095 articles. (3) Further, articles with irrelevant keywords such as “computer software”, “project management”, “robotics”, “computer simulation” were excluded and 628 possible targeted papers remained. (4) This number of articles was still quite large for manual analysis that is why the articles which were also related to such subject areas as “mathematics”, “physics”, “business”, “environment”, etc. were additionally excluded. After that already 187Evaluation of a Novel Approach for Automated Inventive Conceptual Design of Building Structures 552documents were obtained that were published in journals in the field of engineering only and met the original query requirements. (5) 100 of those works were cited at least once that is why they were accepted for further manual analysis. However, after reading all the titles and abstracts carefully it had been discovered that quite many of those journal papers were not in the area of the research interest because they were dedicated to, for instance, automation of construction processes, environmental issues in construction, construction machines and logistics, etc. (6) Finally, 21 articles with appropriate titles were filtered out for reading and analyzing their full texts. All the papers were published during last 10 years and almost half of them were originated from the United States and the United Kingdom.3.2 State of the ArtDetailed review of the discovered literature showed that automation of design for construction is still a relevant topic and building information modeling is one of its key drivers. For instance, in Ref. [5] by Becerik-Gerber and Kensek the emerging trends of building information modelling in architecture, construction and engineering were formulated by performing a survey among practitioners and students. The studied respondents in the work have identified such area as “BIM for Design & Engineering” as one of the most relevant for further research. Moreover, majority of respondents identified the role of BIM in decision making on structural configuration, system choice, etc., as a topic of possible interests. “Linking BIM to analysis tools” is also an emerging research direction. Another similar research was performed five years later by Yalcinkaya and Singh [6]. Principal research areas were revealed that indicate the patterns and trends in BIM research. “Architectural design and design decision making” as well as “Impact of BIM on design creativity and innovation”, “BIM at pre-design phase”, “Parametric modeling and design” and “BIM-supported structural analysis and design” are among them. According to the survey performed by Abrishami et al. [7] majority of the respondents have highlighted that integration of BIM and Generative Design would help to overcome many difficulties during early design stages. Moreover, most of the respondents agree that computational idea generation enhance designers’ capabilities. However, it has been revealed that none of existing systems are fully capable for purposefully manipulating conceptual design. As a result, a framework was proposed that uses generative design for conceptual design and form generation coupled with advanced BIM features for illustration, collaboration, and parametric change management. Another interesting research was done by Robertson and Radcliffe [8] regarding CAD (computer-aided design) tools and their impact on creative problem solving in engineering design. Based on a survey it was found that if CAD was used early in the design process, it was often used in an unstructured way, with the aim of trialing and visualizing alternative ideas. Hence, it was concluded that the CAD developers must change their approach to supporting conceptual design.Some scholars have also highlighted advantages of using TRIZ tools for seeking radical innovations of construction technologies. For instance, in Ref. [9] the function modeling tool was applied in order to design a self-evolutionary model for automated generation of innovative technology alternatives. It was concluded that technology characteristics should be translated into a model that is operational for computer‐aided innovation. Moreover, such a technique as Lean [10] also found its implementation in BIM in construction. Such synergy could improve construction design processes.Bernal et al. [11] focused their research on computational support for designers and identified the areas for future research. According to their results “current computational tools are design-centric, with interfaces from the perspective of the physical components, rather than designer-centric, with a focusEvaluation of a Novel Approach for Automated Inventive Conceptual Design of Building Structures 553on supporting the actions that designers execute while they manipulate the patterns that drive the arrangement of the parts”. Computer-aided decision making in construction project development is also vital according to Ksiązek et al. [12]. According to this research team, experts with extensive knowledge of construction industry take subjective decisions related to verbal methods of decision making. Additionally, importance of decision making on the early building design stage was analyzed by a number of scholars. For instance, Østergård et al. [13] concluded that most of design tools are still evaluative, give little or no guidance, these tools typically provide deterministic results that evaluate the design rather than guide the design proactively. The study [14] by Petersen and Svendsen also confirms that the early stages of building design include a number of decisions which have a strong influence on the performance of the building throughout the rest of the design and construction processes. As a result, a method and a program were developed in order to reduce the need for design iterations, reducing time consumption and construction costs. The program was more regarded to energy consumption and indoor environment of buildings.Parametric scripting is believed to be a productive tool that my help to integrate decision making tools into either BIM or CAD software. According to Nembrini et al. [15] parametric scripting has a strong potential for generating and exploring early design variants. Using such a technique, designers are able to automate geometric description and modification of architectural form. Moreover, Negendahl [16] concludes that combination of a design tool and a visual programming language can provide better support for the designer during the early stages of design. Also dealing with topological information in BIM is an integral part of the conceptual design. Paul and Borrmann [17] have presented concepts of an approach that combines relational database design principles with algebraic and point set topology and shows how complexes and topological spaces can be stored in relational databases. To make complexes suitable for building modelling it is necessary to extend them by geometric properties.On the other hand, a number of researchers have also developed different approaches for conceptual design of structures. Some approaches were presentedin form of theoretical methodologies, while for someof them the ways of automation, including BIM based, were suggested. For instance, Brown and Mueller [18] suggested using geometric multi-objective optimization for design for structural and energy performance of long span buildings. Fenton et al. [19] developed an approach for using grammatical evolution for automatic innovative truss design. Laefer and Truong-Hong [20] have concentrated on automatic generation of 3D steel structures for building information modelling. Afzali et al. [21] developed a procedure based on the MHBMO (modified honey bee mating optimization) algorithm. The technique was developed for discrete optimization of steel frames during design. Another study related to BIM-based structural framework optimization was done by Song et al. [22] who designed a hierarchical structure of a structural framework, described calculation for optimizing structural frame work construction and developed a BIM-based structural framework optimization and simulation system. Tuhus-Dubrow and Krarti [23] developed a genetic-algorithm based approach to optimize building envelope design for residential buildings. The simulation-optimization tool was developed and applied to optimize building shape and building envelope features.Additionally, some studies related to automated layout design and creating conceptual drawings were obtained during the review. Those aspects are definitely important since they represent results of the conceptual design to a finite user or, more likely, customer. Nimtawat and Nanakorn [24] developed a genetic algorithm for automated layout design ofEvaluation of a Novel Approach for Automated Inventive Conceptual Design of Building Structures 554beam-slab floors. They identified the roles of engineers and computers in the design tasks and extracted tasks in structural design of buildings that can be automated and delegated to computers. Kwonet al. [25] developed a novel principle of shape generation.3.3 Literature Review SummaryThe review and analysis of relevant literature has shown that the BIM technology has been already developed significantly. However, there is still a considerable number of emerging trends in evolutionof BIM. Computer-aided decision making, integrationof BIM and generative design, creative problem solving in engineering design, automated innovation of construction technologies, computer-aided decision-making in construction, etc. are among them. Nowadays developers are also focused on increasing the level of automation. More and more functions are automated in modern software. However, the focus is shifted mainly towards detailed design, where the software is only a tool in the implementation of already made decisions. At the stage of conceptual design the level of automation is still quite low. Although this stage of design is fundamental and the level of its computer-aided support should increase. Unfortunately, developed techniques do not consider, for instance, analysis and solving technical contradictions that may often lead to new inventive solutions in design. Furthermore, analysis of conceptual model`s elements functionality does not receive proper development. The level of functionalityof the system’s elements should be determined at the earliest stages of design in order to identify and eliminate the least functional elements as early as possible. This allows designers to obtain the most progressive and optimized solutions and save resources at the subsequent stages of the life cycle of the project. Those conclusions formed the basis for creating a novel approach for automated conceptual design of building structures. 4. A Novel Approach for Automated Conceptual Design of Building Structures After analyzing all advantages and disadvantages of existing techniques in the field of automated conceptual design of building structures a novel methodology that consists of three key steps was proposed in this study. However, those steps are conditional and for a software user they are realized via new functions that are integrated into a BIM software, particularly, Revit©. As this paper is dedicated to evaluation of the already developed approach, it is not described here in very details and only the common logic is provided. Thus, the methodology consists of three key steps in order to simplify and automate complex decision making process on early design stages.The design starts with the step #1 that is called “shape optimization”. Here the only designer’s knowledge, skills and experience are the moving forces. This is the reason why computational support is highly required at this stage. For achieving that purpose special scripts by using a graphical programming software Dynamo have been developed. Graphical programming is a new approach in building design and it brings completely new possibilities for designers and customers and allows significantly automate early design stage. This step is not supposed to use any problem-solving techniques, hence, it is more about looking for optimal solutions but still has a significant meaning for design process. For instance, simple search for an optimal shape of a building only by changing its initial parameters (size, high, number of floors, etc.) instead of completely new design with new parameters of a structure would allow designers to significantly reduce time when project schedules are tight. Once creating a program for a building or a structure a designer can “play” with its initial parameters in order to obtain optimal or desired ones. All that would support engineers during design, shorten conceptual design stage time and accelerate decision making process.Evaluation of a Novel Approach for Automated Inventive Conceptual Design of Building Structures 555When a required shape of a structure is obtained and there are technical contradictions to be solved in the system, a designer can start the next step #2 which is called the “contradiction analysis”. Technical contradiction is a situation when improvements of one feature of the system decrease some its other features. Here the TRIZ Contradiction Matrix with 40 inventive principles is suggested to be used. By clicking on an element users go to the “contradiction matrix” menu and formulate technical contradictions for chosen part of design. After that designers are proposed to use a number of principles in order to solve those contradictions. For instance, for horizontal bearing structures a quite common contradiction is between strength and weight of elements. In this case a sufficient inventive principle is “Composite Materials”—change from uniform to composite (multiple) materials. Indeed, using composite materials in construction (reinforced concrete, bi-steel beams and trusses, etc.) is quite common approach. However, it is up to engineers’ decision and experience to either accept or decline suggested inventive principles in order to improve the design. In case if new inventive conceptual solutions were gained during such analysis, designers can go back to the step #1 for re-optimization.Final step #3 in the proposed approach is the “Function analysis” of a building information model. Here software is supposed to self-analyze BIM and suggest solutions in order to improve the system. For that purpose, it is suggested to use the TRIZ functional modeling and rules of trimming. For increasing software functionality to extracting a function model from BIM model a special graphical program or script with help of Dynamo has been built. The script first automatically detects elements or components in BIM model and places them into an interaction matrix. The interaction matrix defines either elements interact with each other or not and shows all interaction between elements of the system. After that the software defines functions of elements in the Interaction Matrix. In building structures this functions are usually “holds”. However, such functions as “bends”, “expands”, “compresses”, “twists” etc. may take place. For identification of interactions the script applies specially developed rules. On the next step a function model diagram is automatically generated from the interaction matrix. This diagram shows a hierarchical structure of the components and the functions between them. Such function analysis helps to eliminate mental and thinking inertia since attention of designers is put only on elements and functions. Also, the software helps to achieve a more complete and convenient workflow for design engineers as all the steps are done within the BIM environment. Finally, having this overview is a prerequisite for applying the other TRIZ tools, such as function ranking and trimming. Trimming is a method from TRIZ used to reduce the amount of system components without losing system functionality. The method is based on transferring functions performed by a component that should be trimmed to another component. The software uses adapted rules of trimming for finding other components to perform this functionality. The component not performing any functions anymore can be removed (trimmed) from the function model without losing any functionality. As a result, the software highlights the best candidates for trimming in the BIM model and a design engineer can either accept or decline those proposals. In the function ranking functions of elements are ranked according their so-called level of usefulness. In order to perform ranking a “target function” (for instance, “carry live load”) has to be identified firstly. The more high rank belongs to the functions that are closer to the target function. So, the software chooses the furthest from the target functions as the candidates for trimming. There are also so-called “harmful functions” like “bends” or “twists” since bent or twisted elements require more materials in order to stay stable rather than tensioned ones and it may be wise to eliminate such function if it is demanded toEvaluation of a Novel Approach for Automated Inventive Conceptual Design of Building Structures 556design cheaper but equally stable structure. Function ranking offers the user a quick overview on the structure of a system and on the importance of distribution of functions. Such analysis during conceptual design enables engineers to automatically analyze the BIM model and easily obtain progressive design avoiding complex processes of topology optimization and structural analysis which are issues for further detailed design.All of the above steps can be repeated as many times as required by the designer. They can also be executed in any sequence, although it is recommendedto apply the described logic.5. Evaluation of the Proposed ApproachIn order to evaluate the proposed approach a set of coherent questions was developed and distributed via internet-based professional societies. Survey methodology is a method for collecting quantitative information needed for further analysis [26].5.1 Survey DesignThe survey followed several coherent aims. One of them was to identify how respondents use BIM technology in their work or study processes. Moreover, we wanted to reveal what software packages are most used by them. Additionally, we needed to understand how respondents are familiar with the TRIZ and what TRIZ tools are most used. Furthermore, the survey was aimed at understanding the degree of using graphical programming in professional activities. The key purpose of the questionnaire was to collect and evaluate respondents’ opinions regarding automationof the conceptual design process in construction projects and, more importantly, test and evaluate the proposed solution and its technical realization in BIM environment.The target group of the survey included engineers, researchers and students who had any relations to design of building structures. They might work or study in this field. The respondents were found mostly via professional or social networks in the internet by posting corresponding messages with a link to the questionnaire in professional forums, societies, student and research communities, etc. Some of the respondents received the direct link to the survey. Approximately one fifth of those who viewed the message responded to the questions.In general, the developed survey consisted of 19 consecutive questions divided into blocks. Questions 1 and 2 were related to age and region of residence of the respondents and did not have any specific purposes. Question number 3 specified whether the respondents had any relations to design of building structures or not. This question served as a filter in order to weed out respondents not belonging to the required field. Questions 4 and 5 were about experience. Those questions helped to analyze how experienced the respondents were and in which stage of building design they mostly took part. Questions 6-11 were dedicated to design tools, software, etc. Those questions were designed in order to understand how the BIM technology is used by the respondents, what software they use, to what extent they are familiar with TRIZ and how it is applied in professional activities as well as the degree of usage of graphical programming in either work or studies. Question 12 was related to design process and frequency of changes in the design. The main block of questions was devoted to automation of conceptual design process. Questions 13-15 were general and asked opinions about benefits of how software can support a user in the design process. Questions 16-19 were offered to answer after a video demonstration of the possibilities of proposed solution for automation of early building design process.5.2 Survey ResultsAs a result 78 responses were received. And 23.1% of the respondents indicated that they are not related to either design or research in the field of building structures. That is why the remaining 76.9% or 60。

A novel approach emphasising

A novel approach emphasising

MOLECULAR AND CLINICAL ONCOLOGY 3: 55-62, 2015Abstract. To ensure reliable surgical margins, intraop-erative frozen section histological analysis (FS) has been performed since October, 2005 as follows: i) the orientation at the anatomical position and extent of the tumor are shared between oral pathologists and oral surgeons using imaging evaluations and pathological pictures and the planned site of sampling for intraoperative FS is confirmed; ii) a tumor team is organized and the team marks the tumor area and sets the resection range to correct the setting errors of the resection range among operators; iii) vital Lugol staining is applied to the lesion prior to tumor resection, the surgical margin is set based on the non-stained region and the extent of the tumor is macroscopically confirmed in the maximum cross‑sectional surface of the resected specimen; and iv) FS is performed using samples from resected specimens to confirm the muco-epithelium and safety margin of the deep stump. The aim of this study was to evaluate the usefulness of our FS method. The treatment outcomes of oral squamous cell carcinoma were retrospectively investigated in patients treated prior to (Group 1) and after (Group 2) the introduction of our FS method. The recurrence rate of the primary lesions was high (17.3%) in Group 1, but decreased significantly in Group 2 (6.9%). Regarding clinicopathological factors, the condition of the surgical margins was associated with recurrence of the primary lesion in Group 1, but not in Group 2. In conclusion, our FS method appears to be useful for resecting tumors with reliable safety margins.IntroductionLocoregional control and treatment outcomes for primary oral cancers and cervical lymph node metastases have improved markedly with improvements in imaging diagnosis, advances in multidisciplinary treatment applying surgical therapy, radiotherapy and chemotherapy and the development of supportive therapies for oral cancer treatment (1-3). However, despite these advances, the primary lesion recurs in several cases. Therefore, control of the primary lesion is a major concern for oral surgeons, as recurrent lesions are difficult to control and markedly compromise the quality of life of the patients. In surgical therapy for oral cancers, the resection range for the primary lesion is determined based on the TNM classification following evaluation of the clinical findings and images from contrast-enhanced computed tomography (CT), contrast-enhanced magnetic resonance imaging (MRI), posi-tron emission tomography-CT and ultrasonography (1). The safety margins of the resected primary lesion are confirmed during surgery by palpation and from intraoperative frozen section histological analysis (FS). However, the resection range varies among operators, the usefulness of FS has not been verified and the primary lesion recurs in several cases. As regards the methods used for evaluating the safety margins of the resected primary lesions, the 2013 guidelines for the treatment of oral cancer (1) described vital Lugol staining as being useful for mucosal lesions in cancer of the tongue. The recurrence rate of the primary lesions was found to be lower among patients for whom the non-Lugol-stained region was included in the resection field compared to those for whom there was no vital Lugol staining in the resected lesions. Although the examination of all the surgical margins of the resected primary lesions in FS is difficult and the scope of evaluation is limited, investigating the presence or absence of residual tumor tissue in the resected margin appears to be useful. Although actual methods for FS are not frequentlyIntraoperative frozen section histological analysis of resection samples is useful for the control of primary lesions in patients with oral squamous cell carcinomaAKIHIKO MIYAWAKI1, HIROSHI HIJIOKA2, TAKAYUKI ISHIDA2, ETSURO NOZOE2,NORIFUMI NAKAMURA2 and RYOICHI OYA11Department of Oral and Maxillofacial Surgery, University Hospital of Occupational and Environmental Health, Kitakyushu, Fukuoka 807‑8556; 2Department of Oral and Maxillofacial Surgery, Field of Oral and Maxillofacial Rehabilitation, Advanced Therapeutics Course, Graduate School of Medical and Dental Sciences,Kagoshima University, Kagoshima, Kagoshima 890-8520, JapanReceived July 9, 2014; Accepted August 22, 2014DOI: 10.3892/mco.2014.409Correspondence to: Dr Akihiko Miyawaki, Department of Oraland Maxillofacial Surgery, University Hospital of Occupational andEnvironmental Health, 1-1 Iseigaoka Yahatanishi-ku Kitakyushu,Fukuoka 807-8556, JapanE-mail: makihiko@clnc.uoeh-u.ac.jpKey words: oral squamous cell carcinoma, control of primarylesion, surgical margins, locoregional recurrence, intraoperativefrozen section histological analysisMIYAWAKI et al: INTRAOPERATIVE FROZEN SECTION HISTOLOGICAL ANALYSIS OF RESECTED OSCC SAMPLES 56reported, a survey of the American Head and Neck Society by Meier et al (4) stated that 76% of their members collected samples for FS from the surgical bed, 14% from the resected specimens and the remaining 10% from both sites. There were no differences in the findings of FS regardless of the sampling site. Black et al (5) reported the actual condition of FS from the viewpoint of the pathologists, stating that the evaluation of the margins was inaccurate, as the anatomical orientation was not labeled in the resected specimens submitted to pathologists, which requires cooperation with the surgeons. Another report stated that FS is inappropriate for routine investigation of the margins for resected oral cancers other than tongue cancer, as the anatomical structure is complicated and anatomical limits mean that surgical access to the tumor site is generally poor (6). However, Wang et al (7) histopathologically exam-ined the surgical margins of resected tumor specimens in FS using samples obtained by excisional biopsy and reported that no patient required additional treatment following surgery. Kurita et al (8) observed cross-sectional preparations of resected tumor specimens under a digital light microscope and reported that evaluation of the deep margin of the tumor was useful. Therefore, although FS was reported to be useful, there is yet no established method. To achieve accurate FS, it is important to share patient information with the patholo-gists, indicate the anatomical orientation of the resected tumor specimens and prepare samples from appropriate sites (9,10). The advantages of FS using samples collected from resected tumor specimens are as follows: The anatomical orientation is readily determined; the distance between the surgical margin and tumor is macroscopically observed in the cross-sectional surface of the resected specimen; reliable sampling from an appropriate region is possible, as the anatomical orientation is readily determined; and the anatomical position of additional tumor resection is accurately reflected in the surgical field when the surgical margin is either close to the tumor or posi-tive (9,10). Based on these advantages, we collected samples from resected tumor specimens for FS.To evaluate the usefulness of our FS system in the control of primary lesions, using methods such as intraoperative vital Lugol staining and FS of surgical specimens, the outcomes of treatment for oral squamous cell carcinoma (OSCC) were retrospectively investigated in patients treated prior to and after the introduction of this FS method to Kagoshima University. Materials and methodsPatient eligibility criteria. The subjects comprised 153 patients with OSCC who underwent radical surgery at the Department of Oral and Maxillofacial Surgery at Kagoshima University between January, 2000 and September, 2011. The patients were divided according to whether they underwent surgery prior to or after adopting FS for the control of primary lesions in October, 2005 as follows: Group 1 (52 patients), treated between January, 2001 and September, 2005; and Group 2 (101 patients), treated from October, 2005 onwards. The pres-ervation of the morphological characteristics of the oral cavity and functions such as mastication, swallowing, speech and esthetics is crucial in the treatment of advanced OSCC (11). Several studies have reported the effect of preoperative chemo-radiotherapy plus radical surgery for advanced squamous cell carcinoma of the oral cavity (11-14). As a result, surgery was performed as the main treatment and chemoradiotherapy was performed as preoperative treatment throughout this period. Surgery comprised en bloc resection of the primary site, with neck dissection in N1 or more advanced cases. Chemoradiotherapy included external beam radiotherapy with a total radiation dose of 30-40 Gy delivered in 10-20 fractions and concurrent chemotherapy using either platinum-containing agents, such as cisplatin or carboplatin, 5‑fluorouracil, or oral S-1. The clinical characteristics of the patients are summarized in Table I. There were no significant differences according to gender, age, primary site, or distribution of T or stage clas-sification between the groups. However, more patients were treated with surgery alone in Group 2 compared to Group 1, as Group 1 included a higher number of advanced cases. The duration of the follow-up ranged from 1 year to 10 years and 8 months (median, 2 years and 8 months).This study was approved by the Ethics Committee of Kagoshima University and written informed consent was obtained from all the included patients.FS. To ensure reliable surgical margins, we have been performing FS for the control of primary lesions since October, 2005 as follows: First, the orientation of the anatomical extent is determined by oral pathologists and oral surgeons based on images obtained by contrast-enhanced CT and MRI and pathological pictures and the planned sampling site for FS is confirmed. Second, a tumor team is organized and marks the tumor area, setting a reliable 1-cm resection range from the mark to correct the setting errors of the resection range by the operators. Third, only the presence or absence of tumor in tissues collected from the surgical bed of the tumor resection site is investigated in FS, but vital Lugol staining is applied (Fig. 1A) and the surgical margin is set based on the non-stained region. The distance from the tumor is macro-scopically confirmed in the maximum cross‑sectional surface of the resected specimen by oral surgeons and pathologists (Fig. 1B and C, white arrows). Finally, FS is performed using a sample collected from the resected specimen to confirm the mucoepithelium and safety margin of the deep stump (Fig. 1D).Items analyzed in the two groups. First, the rates of posi-tive surgical margins, recurrence of the primary lesion and disease‑specific survival were compared. Second, the clini-copathological factors associated with recurrence of primary lesions were analyzed. The investigated clinicopathological factors included age, gender, tumor location, T classification, tumor properties, grade of differentiation, invasion pattern, presence or absence of lymphatic, vascular, or nerve invasion, condition of the surgical margins and histological therapeutic effect. The patients were divided by age into those aged ≥61 and those <60 years, by T classification into T2 or lower and T3 or more advanced cases, by grade of differentiation into moder-ately or poorly differentiated and well-differentiated cases and by condition of the surgical margins into cases with residual tumor (positive margins), without residual tumor but ≤3 mm from the tumor, or without residual tumor and >3 mm from the tumor (negative margins). The invasion pattern was classified as YK3 or lower and YK4C or more advanced, according toMOLECULAR AND CLINICAL ONCOLOGY 3: 55-62, 201557the classification reported by Yamamoto et al (15). As regards the histological therapeutic effect, recurrence of the primary lesion was evaluated in patients who received preoperative therapy by dividing them into cases with Gr2a or lower and Gr2b or higher effects, according to the classification reported by Shimosato et al (16). Third, disease‑specific survival rates were compared between the groups according to the condition of the surgical margins. Finally, the primary site, condition of the surgical margin, time of recurrence and prognosis were analyzed in cases with recurrence of the primary lesion in Groups 1 and 2.Statistical analysis. Statistical analysis was performed using JMP® statistical analysis software, version 9 (SAS Institute, Tokyo, Japan). The associations between recurrence rate and clinicopathological factors were analyzed using the Pearson's χ2 test. The survival rates were calculated using the Kaplan-Meier method and analyzed using the log-rank test. P<0.05 was considered to indicate a statistically significant difference.ResultsComparison of surgical margin positivity, primary lesion recurrence and disease‑specific survival by the Kaplan‑Meier method. The surgical margin positivity rates were 9.6 and 3.9% in Groups 1 and 2, respectively, with a decreasing tendency, although the difference was not statistically significant (Table II). The recurrence rate for primary lesions was high (17.3%, 9/52) in Group 1, but improved significantly to 6.9% (7/101) in Group 2 (Table II). Disease‑specific survival rates were 81.5 and 87.9% in Groups 1 and 2, respectively, showing a slight but non‑significant tendency toward improvement (Fig. 2).Clinicopathological factors associated with recurrence of the primary lesions. The Pearson's χ2 test was performed regarding the presence or absence of recurrence of the primary lesion as a response variable and gender, age, location, T clas-sification, tumor properties, grade of differentiation, invasion pattern, presence or absence of lymphatic, vascular, or nerve invasions, condition of the surgical margins and histological therapeutic effect as explanatory variables. In Group 1, factors associated with recurrence of the primary lesion were the presence or absence of nerve invasion and the condition of the surgical margins; recurrence rate was found to be significantly higher among cases with surgical margins close to the tumor or residual tumor in the surgical margins (positive margins). In Group 2, none of the explanatory factors were significantly associated with the presence or absence of recurrence of the primary lesion. Regarding the association between primaryTable I. Clinical characteristics of patients.Group 1, no. (%) Group 2 , no. (%) Total patient no. (%) Characteristics (n=52) (n=101) (n=153) GenderMale 32 (38.5) 60 (40.6) 92 (60.1)Female 20 (61.5) 41 (59.4) 61 (39.9) Age (years)<60 13 (25.0) 33 (32.7) 46 (30.0)≥61 39 (75.0) 68 (67.3) 107 (70.0) Primary siteUpper gingiva 6 (11.5) 10 (9.9) 16 (10.5)Tongue 23 (44.2) 52 (51.5) 75 (49.0)Lower gingiva 16 (30.8) 30 (29.7) 46 (30.0)Other 7 (13.5) 9 (8.9) 16 (10.5) Clinical T classificationT1/2 37 (71.2) 83 (82.2) 120 (78.4)T3/4 15 (28.8) 18 (17.8) 33 (21.6) StageI 10 (19.2) 18 (17.8) 28 (18.3)II 12 (23.1) 40 (39.6) 52 (34.0)III 19 (36.5) 24 (23.8) 43 (28.1)IV 11 (21.2) 19 (18.8) 30 (19.6) TreatmentS 8 (15.4) 54 (53.4) 62 (40.5)R→S 21 (40.4) 5 (5.0) 26 (17.0)R+C→S 23 (44.2) 42 (41.6) 65 (42.5) S, surgery; R, radiotherapy; C, chemotherapy.MIYAWAKI et al : INTRAOPERATIVE FROZEN SECTION HISTOLOGICAL ANALYSIS OF RESECTED OSCC SAMPLES58site and recurrence of the primary lesion, primary lesions in the upper and lower gingiva frequently recurred in both groups, but the incidence decreased in Group 2 and cancer of the tongue recurred in only 1 patient (Table III).Disease‑specific survival rate by condition of the surgical margins in Groups 1 and 2. In Group 1, the survival rate was 87.8% in cases with negative surgical margins, 72.8% in cases with margins close to the tumor and 60.0% in cases withpositive margins. In Group 2, the survival rates of cases with negative margins and cases with margins close to the tumor were 93.3 and 78.3%, respectively, exhibiting a tendency toward higher rates compared to those in Group 1, although the differences were not significant. The disease-specificsurvival rate in positive-margin cases was 50.0%, which wasFigure 1. Intraoperative frozen section histological analysis method. (A) A case of T1 cancer of the tongue. Vital Lugol staining was applied during surgery and the surgical margins 10 mm from the tumor were determined. (B) Resected tumor specimen. The specimen was cut in cross-section (black line) in the center of the tumor (region circled with white dotted line) with a palpable induration. The white arrows show the distance between the surgical margins and the tumor macroscopically. (C) The cross-sectional surface of the tumor was observed macroscopically to evaluate the surgical margins (the white dotted line represents the tumor margin). The white arrows show the distance between the surgical margins and the tumor. (D) Hematoxylin and eosin staining (magnification, x200). A sample was collected from the deepest region close to the macroscopic tumor and subjected to intraoperative rapid pathological examination. The white arrows shows the distance between the region demarcated by the white dotted line and the surgical margins microscopically.Table II. Rates of negative surgical margins and recurrence at primary site in Groups 1 and 2.Variables Group 1 Group 2 P-valueMargins Positive47 97 Negative (%) 5 (9.6) 4 (3.9) 0.16Recurrence No 43 94 Yes (%)9 (17.3)7 (6.9)0.047aaP<0.05 (Pearson's χ2test).Figure 2. Disease‑specific survival rate s in Groups 1 and 2.MOLECULAR AND CLINICAL ONCOLOGY 3: 55-62, 201559lower compared to that in Group 1. Significant differences according to the condition of the surgical margins were noted in the survival rates of both groups (Fig. 3).Patients with recurrence of primary lesions in Groups 1 and 2 and outcome. In Group 1, the primary tumors recurred in 9 of the 52 patients (17.3%). By primary site, recurrenceTable III. Clinicopathological factors associated with recurrence at primary site.Group 1 Group 2--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Recurrence Recurrence Variables No recurrence no. (%) P-value No recurrence no. (%) P-value GenderMale 28 4 57 4Female 15 5 0.25 37 3 0.36 Age (years)≥61 12 1 32 1<60 31 8 0.29 62 6 0.28 Primary siteUpper gingiva 4 2 (33.3) 8 2 (20.0)Tongue 20 3 (13.0) 51 1 (1.9)Lower gingiva 13 3 (18.8) 26 4 (13.3)Other 6 1 0.56 9 0 0.12 Clinical T classificationT1/2 32 5 78 5T3/4 11 4 0.26 16 2 0.44 Pattern of tumor growthSuperficial spreading 6 0 22 3Outgrowing 2 0 24 1Ingrowing 35 9 0.37 48 3 0.49 DifferentiationModerate/poor 29 7 81 7High 14 2 0.54 13 0 0.29 Mode of invasion b≤YK3 36 6 76 4YK4C/4D 7 3 0.24 18 3 0.16 Lymphatic invasionNegative 39 7 82 6Positive 4 2 0.27 11 1 0.85 Vascular invasionNegative 37 7 76 6Positive 6 2 0.53 17 1 0.79 Nerve invasionNegative 42 7 86 7Positive 1 2 0.02a 7 0 0.45 Surgical marginNegative 32 3 73 4Close (<3 mm) 9 3 17 3Positive 2 3 0.01a 4 0 0.21 Chemoradiation effect c≤Gr2a 11 5 12 2≥Gr2b 22 3 0.13 30 1 0.17 a P<0.05 (Pearson's χ2 test). b C lassification reported by Yamamoto et al (15). c C lassification reported by Shimosato et al (16).MIYAWAKI et al : INTRAOPERATIVE FROZEN SECTION HISTOLOGICAL ANALYSIS OF RESECTED OSCC SAMPLES60occurred in the upper gingiva in 2 patients, tongue in 3, lower gingiva in 3 and buccal mucosa in 1 patient. The T classifica -tion varied between T1 and T4 and the surgical margins were negative, close to the tumor and positive in 3 patients each. The recurrence site was the tongue, gingiva, buccal mucosa and retromolar mucosa around the primary site in 6 patients and the tumor advanced into the skin and recurred in 3 patients. The time to recurrence was between 1 and 3 months in cases with positive margins, after 5 months in 2 cases with close margins and significantly later in cases with negative margins (range, 1 year and 5 months to 3 years and 11 months).The treatment comprised tumor resection or chemotherapy in 8 patients and 5 patients (62.5%) survived, but the outcomes were poor and 4 patients (37.5%) succumbed to the primary tumor.In Group 2, the primary lesions recurred in 7 of the 101 patients (6.9%). The primary site was located in the upper and lower gingiva in 6 cases and in the tongue in 1 case. The T classification was late T2 or more advanced and the surgical margins were negative in 4 and close to the tumor in 3 cases ; however, no positive cases were recorded. The site of recurrence was the tongue, gingiva and buccal mucosa around the primary lesion in 4 patients and the skin in 3 patients. The time to tumor recurrence was 4-7 months in cases with close margins, >1 year in 2 cases with negative margins, but only 3 months after surgery in 1 case with negative margins. The treatment comprised radio-therapy or resection in 6 patients, of whom 3 (50%) survived and 3 succumbed to the primary lesion. One patient with lower gingival cancer was untreatable and eventually succumbed to the disease. The characteristics of the cases with recurrence of the primary tumor are summarized in Table IV.Figure 3. Disease‑specific survival rate according to the surgical margin in Groups 1 and 2.Table IV . Cases of recurrence at primary site and prognosis. Age Primary TN Surgical Site of Time to SalvageCase (years) Gender site stage margins recurrence recurrence treatment OutcomeGroup 1 1 52 Female Upper gingiva T2N1 Close Skin3y 2m Excision Alive 2 63 Male Upper gingiva T3N0 Close Buccal mucosa 5m Excision Alive 3 70 Female Tongue T1N0 Negative Tongue 3y 11m Excision Alive 4 67 Male Tongue T2N0 Negative Tongue 1y 5m Excision Deceased 5 71 Male Tongue T2N1 Negative Skin3y ExcisionAlive 6 62 Male Lower gingiva T4N2b Positive Retromolar 3m Chemotherapy Alive 7 68 Female Lower gingiva T2N0 Positive Skin 1m -Deceased 8 86 Female Lower gingiva T2N1 Close Gingiva5m Chemotherapy Deceased 9 84 Female Buccal mucosa T3N0 Positive Buccal mucosa 1m Excision Deceased Group 2 10 66 Male Upper gingiva T2N2b Negative Buccal mucosa 1y Radiotherapy Alive 11 84 Female Upper gingiva T3N0 Close Skin 7m Excision Deceased 12 81 Female Tongue T4N0 Negative Tongue 1y Radiotherapy Deceased 13 72 Male Lower gingiva T4N1 Close Skin 4m Excision Deceased 14 81 Female Lower gingiva T2N0 Negative Skin 3m Excision Alive 15 84 Female Lower gingiva T4N0 Close Gingiva 5m -Deceased 1660FemaleLower gingiva T2N0 NegativeGingiva1y 9mExcisionAliveY , years; m, months.MOLECULAR AND CLINICAL ONCOLOGY 3: 55-62, 201561DiscussionThe major clinical factor determining the prognosis of patients with OSCC is cervical lymph node metastasis, whereas the depth and pattern of invasion are important factors associ-ated with recurrence of the primary lesion and lymph node metastasis (1). In addition to the depth and invasion pattern of the tumor, the presence or absence of tumor cells in the surgical margins is crucial for the surgical treatment of OSCC (17,18). Setting a safety margin ≥10 mm is considered as appropriate for the resection of oral cancers, although a clear basis for this distance is currently lacking (19). We have attempted to control primary lesions by following this criterion (10‑mm safety margin), confirming that the region remains unstained on vital Lugol staining during surgery and including this region in the resection field, confirming the macroscopic tumor extent in the cross-sectional surface of the resected specimen and performing FS for a sample collected from the resected specimen. Although the disease‑specific survival rate was not significantly affected, the rate of positive surgical margins was decreased. The rate of primary lesion recurrence was high (17.3%, 9/52) in Group 1, but improved significantly to 6.9% (7/101) in Group 2. Among the clinico-pathological factors, the condition of the surgical margins and the presence or absence of nerve invasion were associated with recurrence of the primary lesion in Group 1, but no significant association between the surgical margin status and recurrence of the primary lesion was observed in Group 2. However, the prognosis of patients with positive margins was poor in both groups and, although the incidence of recurrent cancer of the tongue tended to decrease, upper and lower gingival cancers recurred in a number of patients, reflecting the limitations to our approach for the control of primary lesions.The number of studies reporting the recurrence rate of primary lesions in detail is limited. Although the rates vary depending on the primary site, Yamamoto et al (18) reported a rate of 10.3% in patients with T1̸2 cancer of the tongue, whereas that of oral cancers of other regions, including the tongue, was reported to be 9-18% by other studies (18,20-22). Although a simple comparison with these reports is not feasible due to the differences in patient background and treat-ment strategy, the rate of primary lesion recurrence was 17.3% in Group 1, which was similar to the previously reported rates, and decreased to 6.9% in Group 2, which was lower compared to the rates reported elsewhere. In addition, among the clinico-pathological factors, the condition of the surgical margins and nerve invasion were associated with recurrence of the primary lesion in Group 1, while no significant correlation was noted between surgical margin status and recurrence of the primary lesion in Group 2. Surgical margin positivity represents a significant factor associated with decreased survival rate and a high risk of postoperative recurrence (1,22). The condition of the surgical margins was significantly associated with survival rate in both groups (Fig. 3), suggesting that our approach for the control of primary lesions contributes to decreasing the risk of recurrence and our FS method appears to be useful for the evaluation of the surgical margins. However, the survival rate did not significantly improve in Group 2 compared to that in Group 1, although a tendency towards an increase was observed. The poor prognosis of patients with cervical lymph node metastasis, including secondary cervical lymph node metastasis in Group 2 (data not shown), may have affected our results.The recurrence rate of the primary lesions varies depending on the primary site. The oral cavity has a complex structure, comprising mixed hard and soft tissues and the invasion pattern varies depending on the direction of tumor advancement. Such factors may contribute to the difficulties in the determina-tion of the resection range with adequate safety margins (1). Recurrence of the primary lesion was frequently noted in the upper and lower gingiva in both groups. This tendency persisted in Group 2, but the incidence was decreased in all the primary sites. As regards cancer of the tongue, a low rate of primary lesion recurrence (3.8%) has been reported (15). In our patients with cancer of the tongue, the rate of primary site recurrence was 13.0% in Group 1, but decreased to 1.9% in Group 2. In Group 2, recurrence occurred in the upper and lower gingiva in 2 and 4 patients, respectively (Table IV), but recurrence in the tongue occurred in only 1 case. The advances in imaging diagnosis may also be a decisive factor when determining the resection range, but the advantages of our FS method (i.e., the cross-sectional surface of tumors is readily observed macroscopically, the distance between the surgical margin and tumor is readily determined and the anatomical orientation is readily identified) is evident in tissues retaining anatomical continuity, such as the tongue, which may facili-tate determining a reliable resection range for cancer of the tongue. In Group 2, although recurrence was negative on intraoperative rapid pathological diagnosis, upper and lower gingival cancers recurred in the surrounding tissue relatively early after surgery (3-7 months) in 4 of the 6 patients. These cases reflect the limitations of our FS method in assisting with determining a reliable tumor resection range, in addition to the difficulties involved in imaging diagnosis of tumors located in regions with a complex anatomical structure, such as advanced upper and lower gingival cancers containing hard as well as soft tissues. The prognosis for cases with recurrence is very poor (23,24). To determine the resection range for the primary lesion in such cases, further improvements are required in the imaging evaluation of jaw bone infiltration, tumor invasion pattern and infiltration into the surrounding soft tissues in consideration of the direction of tumor advancement (25).In conclusion, our FS method appears to be useful for resecting tumors with reliable safety margins for tissues retaining anatomical continuity, such as the tongue. The macroscopic observation of cross-sections of the resected tumor specimens is easy and the surgical margins may be readily investigated. However, this method is insufficient for determining a resection range in tissues containing soft tissue and jaw bone, such as upper and lower gingival tumors, and other methods to control primary lesions must be investigated. AcknowledgementsThe authors would like to thank the members of the Department of Oral and Maxillofacial Surgery, Field of Oral and Maxillofacial Rehabilitation, Advanced Therapeutics Course, Graduate School of Medical and Dental Sciences, Kagoshima University, for their assistance with additional data collection.。

放大内镜和窄带成像技术发现早期胃癌

放大内镜和窄带成像技术发现早期胃癌

放大内镜和窄带成像技术发现早期胃癌(组织分型及浸润深度)济南军区总医院消化科吴文明刘晓峰孙自勤VS 经典分型在区分癌与非癌上发挥了重要作用,后期学者又进一步对MV/MS 分型细化,以期预测组织学分型和早癌浸润深度,从而为后期内镜或外科治疗提供决策。

在预测组织学分型上,2010 年Akira 推出基于MV 的胃早癌ME+NBI 分型,即FNP (精细网格,fine network pattern),CSP(螺旋型,corkscrew pattern)(图1),此前一直被定义为「未分类型」的表型被进一步细化为IIL-1(小叶内环型-1,intra-lobular loop pattern-1),ILL-2(小叶内环型-2,intra-lobular loop pattern-2)(图2)。

根据研究结果,FNP 与ILL-1 大部分为分化型腺癌,而CSP 多为未分化型腺癌。

ILL-2 主要分布于分化型腺癌,但也可在未分化腺癌中存在。

此外,64% 未分化ILL-2 腺癌中可见CS(螺旋状)微血管(图3),但在分化腺癌中未见此现象发生。

图1 FNP 与CSP 分型。

A FNP 为MV 的精细网络状结构;B CSP 为MV 的螺旋型结构图2 ILL-1 与ILL-2 分型。

A ILL-1 为包含环形MV 的绒毛状腺体结构;B ILL-2 为上述基础上出现腺体断裂图3 IIL-2 型胃癌中出现CS 型MV。

A 胃体中部后壁一0-IIc 型早癌;B NBI 可见不规则茶色区域;C ME+NBI:断裂绒毛状腺体上可见CS 型MV;D 病理示黏膜固有层未分化腺癌相反,Masashi 等另辟蹊径,从癌灶周围非癌黏膜表型推测癌灶组织学类型,将癌灶周围非癌黏膜的ME+NBI 表现分为以下四型:A 点状型(圆形或针眼点状);B 短线状(扩张、垂直长凹痕结构);C 条纹型(管样,小梁嵴样结构);D 颗粒型(绒毛、乳头状结构)(图4)。

高效视频编码(HEVC)帧内预测块尺度的快速选择方法

高效视频编码(HEVC)帧内预测块尺度的快速选择方法

高效视频编码(HEVC)帧内预测块尺度的快速选择方法邱春婷;齐静【摘要】针对高效视频编码(high efficiency video coding,HEVC)中帧内尺度模式选择计算复杂度高的问题,提出了一种HEVC帧内预测块尺度的快速选择方法.基于图像的纹理复杂度,通过提前检测帧内预测块的绝对误差均值,选择预测块尺度类型,减少穷尽搜索代价计算过程.实验结果表明,该方法能够有效降低帧内预测块尺度模式选择的计算复杂度,在比特率平均提高3.09%的情况下,编码时间能平均减少29.87%,峰值信噪比平均提高0.031%.【期刊名称】《科学技术与工程》【年(卷),期】2014(014)018【总页数】4页(P64-67)【关键词】HEVC;帧内预测;模式选择;纹理复杂度【作者】邱春婷;齐静【作者单位】西安工程大学服装与艺术设计学院,西安710048;西安工程大学服装与艺术设计学院,西安710048【正文语种】中文【中图分类】TN919.8新一代高效视频编码技术(high efficiency video coding,HEVC)[1],由视频编码联合协作小组(JCT-VC)共同开发。

2013年1月,HEVC 的第一版最终草案(Main Profile、Main 10 Profile、Main 静止图像 profile)获得 ITU-T 批准,并被命名为 H.265。

HEVC遵循H.264/AVC的核心机制,并采用了多种新的编码技术,显著提高了编码性能[2]。

与H.264/AVC High Profile相比,HEVC可以在保证图像质量不受影响的前提下将码率降低50%,但这是以增加编码计算复杂度为代价的。

如同H.264/AVC,帧内编码是HEVC的重要组成部分,主要利用了帧内预测,残差的空间变换,系数量化和熵编码机制[3]。

在这种机制中,一个有效的帧内预测会产生小的残差并提高编码效率。

因此,帧内预测在HEVC中仍然起着非常重要的作用。

拓扑理论

拓扑理论

The topological approach to perceptual organizationLin ChenKey Laboratory of Cognitive Science,Graduate School and Institute of Biophysics,Chinese Academy of Sciences,Beijing,ChinaTo address the fundamental question of``what are the primitives of visual per-ception'',a theory of topological structure and functional hierarchy in visual perception has been proposed.This holds that the global nature of perceptual organization can be described in terms of topological invariants,global topological perception is prior to the perception of other featural properties,and the primitives of visual form perception are geometric invariants at different levels of structural stability.I n Part Iof this paper,Iwill illustrate why and how the topological approach to perceptual organization has been advanced.In Part II,I will provide empirical evidence supporting the early topological perception,while answering some commonly considered counteraccounts.In Part III,to complete the theory,I will apply the mathematics of tolerance spaces to describe global properties in discrete sets.In Part IV,I will further present experimental data to demonstrate the global-to-local functional hierarchy in form perception,which is stratified with respect to structural stability defined by Klein's Erlangen Program.Finally,in Part V,Iwill discuss relations of the global-to-local topological model to other theories:The topological approach reformulates both classical Gestalt holism and Gibson's direct perception of invariance,while providing a challenge to com-putational approaches to vision based on the local-to-global assumption.INTRODUCTIONA great divide:Local-to-global vs.global-to-localAs a Chinese proverb says:Everything is difficult at its very beginning. Historically,major schools of vision diverge in their answers to the question of ``Where visual processing begins?''(Pomerantz,1981)or``What are the pri-mitives of visual perception?''(Chen,1982).The question is so fundamental and also so controversial as to serve as a watershed,a Great Divide,separatingPlease address all correspondence to:Lin Chen,Key Laboratory of Cognitive Science,Graduate School and Institute of Biophysics,Chinese Academy of Sciences,15Datun Road,Beijing100101, China.Email:chenl@Supported by National Nature Science Foundation of China Grant(697900800);Ministry of Science and Technology of China Grant(1998030503);Chinese Academy of Sciences Grants (KGCX2-SW-101,KJCX1-07).This work was partly done during author's sabbaticals,at Institute of Medical Psychology,University of Munich,and at National Institute of Mental Health./journals/pp/13506285.html DOI:10.1080/13506280444000256554CHENtwo most basic and sharply contrasting lines of thinking in the study of perception.Early feature analysis:Local-to-global.On one side of the Great Divide,the early feature-analytic viewpoint holds that perceptual processing is from local to global:Objects are initially decomposed into separable properties and components,and only in subsequent processes are objects recognized,on the basis of the extracted features.The computational approach to vision by Marr (1982),representative of``early feature-analysis''viewpoint,claims that the primitives of visual-information representation are simple components of forms and their local geometric properties,such as,typically,line segments with slopes. Early holistic registration:Global-to-local.On the other side of the Great Divide,the viewpoint of early holistic registration claims that perceptual processing is from global to local:Wholes are coded prior to perceptual analysis of their separable properties or parts,as indicated by the conception of perceptual organization in Gestalt psychology.As we will see in the following discussion,with respect to the fundamental question of``Where to begin'',the core contribution of the Gestalt idea goes far beyond the notion that``Whole is more than the simple sum of it parts'';rather it is that``Holistic registration is prior to local analysis''.The idea of early feature analysis has gained wide acceptance,and dominates much of the current study of visual cognition.Intuitively,it seems to be natural and reasonable that visual processing begins with analysing simple components and their local geometric properties,typically as line segments with slopes,as they are readily to be considered physically simple and computationally easy. An underlying idea of Marr's computational system of vision was,in Marr's own words,``In the early stages of the analysis of an image,the representations used depend more on what it is possible to compute from an image than on what is ultimately desirable.''(Marr,1978).Nevertheless,a starting point of the present paper is that physically or computationally simple doesn't necessarily mean psychologically simple or perceptually primitive;therefore,the question of which variables are perceptual primitives is not a question answered primarily by logical reasoning or analysis of computational complexity but rather by empirical findings.Topological structure and functional hierarchyinform perceptionTo address the fundamental question of what are the primitives of visual percep-tion,based on a fairly large set of data on perceptual organization reviewed here,a theory of``early topological perception''has been proposed.This holds that:GLOBAL TO LOCAL TOPOLOGICAL PERCEPTION555A primitive and general function of the visual system is the perception of topo-logical properties.The time dependence of perceiving form properties is system-atically related to their structural stability under change,in a manner similar to Klein's hierarchy of geometries;in particular,topological perception(based on physical connectivity)is prior to the perception of other geometrical properties.The invariants at different geometrical levels are the primitives of visual form perception.These include,in a descending order of stability(from global to local), topological,projective,affine,and Euclidean geometrical invariants.The topological approach is based on one core idea and includes two main aspects.The core idea is that perceptual organization should be understood in the perspective of transformation and perception of invar-iance over transformation.The first aspect emphasizes the topological struc-ture in form perception,namely,that the global nature of perceptual organization can be described in terms of topological invariants.The sec-ond aspect further highlights early topological perception,namely,that topo-logical perception is prior to perception of local featural properties.The ``prior''has two strict meanings:First,it implies that global organizations, determined by topology,are the basis that perception of local geometrical properties depends on;and second,topological perception(based on physi-cal connectivity)occurs earlier than the perception of local geometrical properties.The hypothesis of early topological perception led to a major finding that the relative perceptual salience of different geometric properties is remarkably consistent with the hierarchy of geometries according to Klein's Erlangen Program(see Part II and IV),which stratifies geometries in terms of their relative stability over transformations.Based on the finding,a functional hier-archy in form perception has been established as a formal and systematic definition of``global-to-local''relations:A property is considered more global (or stable)the more general the transformation group is,under which this property remains invariant;relative to geometrical transformations,the topolo-gical transformation is the most general and hence topological properties are the most global.The framework of the topological structure and functional hierarchy high-lights a fundamental empirical prediction,namely a time dependence of per-ceiving form properties,in which visual processing is from global to local:The more global a form invariant is the earlier its perception occurs,with topological perception being the most global and occurring earliest.The framework further highlights a series of novel empirical predictions about long-standing issues related to the study of perceptual organization,and many Gestalt-type phe-nomena in form perception may be explained in this unified manner.They include the following examples:556CHEN.With respect to the relationship between different organizational factors, proximity is the most fundamental organizational factor(even in comparison with uniform connectivity;Palmer&Rock,1994)(see Part III),and there is a time course of processing different organizational principles:Proximity precedes similarity,and topological similarity precedes similarity of local geometric properties(see Part VI)..Early topological perception predicts the visual sensitivity to distinction made in topology.For example,two stimuli that are topologically different are more discriminable under a near-threshold condition than are other pairs of forms that are topologically equivalent despite their difference in local features(see Part II)..With respect to the question of whether motion perception precedes form perception or vice versa,topological discrimination should occur earlier than and determine motion perception(see Part II)..Configural superiority effects(Pomerantz,1981)demonstrated by configural relations between line segments,such as the``triangle±arrow pair'',may simply demonstrate the superiority effect for perception of holes over indi-vidual line segments(see Part IV)..With respect to``global precedence''(Navon,1977),according to the functional hierarchy,the performance advantage for compound letters is quite straightforward:Global precedence reflects the primacy of proximity in perceptual organization(see Part III)..If topological properties are primitive,illusory conjunctions(Treisman& Gelade,1980)of topological properties,such as holes,should sometimes take place(see Part II)..With respect to the definition of perceptual object,the topological approach ties a formal definition of``object''to invariance over topological trans-formation(see Part I).From this definition,it follows that as an object is moving along and a hole appears in it,this topological change would dis-turb object continuity,while changes of shape and colour wouldn't(Wolfe, personal communication).For example,in an MOT(multiple object track-ing)test(Pylyshyn&Storm,1988;vanMarle&Scholl,2003),attentive tracking processes would be impaired by objects changing topology by getting a hole,while it does not matter if they change local features and colours..With respect to its ecological function and functional anatomy,long-range apparent motion works by abstracting form invariants,and hence is asso-ciating with form perception and activates the ventral pathway in the two visual systems(Ungerleider&Mishkin,1982).Specifically,the fMRIacti-vation should be correlated with the form stability under change(see Parts II and IV)..From the perspective of biological evolution,if topological perception is indeed a fundamental property of vision,one might expect topologicalGLOBAL TO LOCAL TOPOLOGICAL PERCEPTION557 properties to be extracted by all visual systems,including the relativelysimple ones possessed by insects,such as bees(see Part II).In summary,the framework of topological structure and functional hierarchy in form perception provides a new analysis of the fundamental question,i.e., ``What are the primitives of visual perception?'',in which primitives of visual form perception are considered to be geometric invariants(as opposed to simple components of objects,such as line segments)at different levels of structural stability.In the following,I will illustrate why and how the topological approach to perceptual organization has been advanced(Part I);provide empirical evidence supporting the topological perception,while answering some commonly con-sidered counteraccounts(Part II);complete the theory of topological perception, using the mathematics of tolerance spaces that describe global properties in discrete sets(Part III);present experimental data to demonstrate the functional hierarchy in form perception,which is stratified with respect to structural sta-bility defined by Klein's Erlangen Program(Part IV);and finally,discuss relations of early topological perception to other theories,including Gestalt psychology,Gibsonian psychology,and the computational approach(Part V).PART I:WHY AND HOWÐA TOPOLOGICALAPPROACH TO PERCEPTUAL ORGANIZATIONA paradoxical problem of``where to put the master map'' Fundamental problems faced by the early feature-analysis approach are typically embodied in a paradoxical problem of``where to put the master map''as posed by the feature-integration theory of Treisman and co-workers(e.g.,Treisman& Gelade,1980).Feature-integration theory,consistent with the early feature-analysis approach,adopts a``two-stage model'':In the first,preattentive stage, primitive features,such as colours and orientations,are abstracted effortlessly and in parallel over the entire visual field,and registered in special modules of feature maps;and in the second,attentive stage,focal attention is required to recombine the separate features to form objects.A master map of locations plays a central role in feature binding by tying the separate feature maps together.Within the master map,a focal attention mechanism selects a filled location,binding the activated features linked to that location together to form a coherent object. Problems for feature-integration theory are,however,represented by the question of where exactly the master map of locations fits into the feature integration mechanism.In Treisman's own words,``I have hedged my bets on where to put the master map of locations by publishing two versions of the figure!In one of them,the location map received the output of the feature558CHENmodules(e.g.,Treisman,1986)and in other is placed at an earlier stage of analysis(e.g.,Treisman,1985;Treisman&Gormican,1988)''(Treisman,1988, pp.203±204).To place the master map of locations at an early stage of analysis,in Treisman's own words,``implies that different dimensions are initially conjoined in a single representation before being separately analysed''(Treisman,1988,pp. 203±204).This contradicts the basic position of early feature analysis.Placing the master map later,however,contradicts some behavioural data.One of the strengths of feature-integration theory is that it draws on a number of major pieces of counterintuitive evidence,including illusory conjunction and visual search, which appear to provide strong support for early feature analysis(e.g.,Treisman &Gelade,1980;Treisman&Gormican,1988).However,it is interesting to see that problems for this theory also arise here(e.g.,for a general review,see Humphreys&Bruce,1989).For example,despite the fact that line segments are commonly considered to be basic features,there is markedly little evidence for illusory conjunction where line segments are miscombined into letter-like objects,when letter shapes and line segments forming the letter shapes are used as stimulus forms(e.g.,Duncan,1984).In contrast,there is much stronger evidence that whole letter shapes migrate across words and produce illusory conjunctions of the entire letter shapes,rather than of line segments making up the letter shapes. These findings indicate that letter shapes,as combinations of line segments, behave psychologically as holistic objects,even though line segments are commonly considered to be basic features.Apparently attention,as it relates to feature binding,is not needed for holistic object perception.This suggests that before a stage of separate featural analysis,there must be a stage of early holistic perception in which objects like letters are coded as wholes.Treisman and co-workers,in their effort to explain these counterexamples, have augmented feature-integration theory with new strategies and new mechanisms of attention,such as``guided search''(e.g.,Wolfe,1994),``map suppression'',and dividing items into different depth planes.Nevertheless,these efforts are not completely successful(e.g.,Duncan&Humphreys,1989)but rather in fact illustrate that,despite the attractions of feature-integration theory, the paradoxical problem of``Where to put the master map''stems directly from the fundamental question of``Where visual processing begins''.Perceptual organization:To reverse back theinverted(upside-down)problem of feature binding Regardless of how an object is decomposed into properties and components,the decomposed features themselves are unlikely to be sufficient for achieving object recognition.Indeed,we do not normally perceive isolated features such as brightness,colours,and orientations free from an object,leading to the con-tention that there must be a further process of feature binding.This problem ofGLOBAL TO LOCAL TOPOLOGICAL PERCEPTION559 feature binding presents a central problem for current vision research in parti-cular,and for parallel and distributed modelling of cognition in general(e.g., MuÈller,Elliott,Herman,&Mecklinger,2001).However,despite the centrality of the issue for perceptual theory,it is questionable whether any breakthrough has been made after extensive efforts. Both spatial and temporal factors have been considered as cues for binding features together.But the principles for feature binding based on either space or time are neither always obeyed nor exclusive.Feature binding and perceptual organization appear to be very similar pro-blems(Duncan,1989)in the sense that both deal with similar processes,such as ``what goes with what'',and with similar concepts,such as belongingness and assignment.It turns out that,even though the early feature-analysis viewpoint emphasizes the fundamental importance of early parallel processing,the issue of perceptual organization remains indispensable.Yet,the concepts of``perceptual organization''and``feature binding''involve very different underlying issues, with the former rooted in the idea of early holistic registration and the latter originating from an assumption of early feature analysis.Thus,with respect to the fundamental question of``Where to begin'',perceptual organization and feature binding can be considered contrary concepts,going in opposite directions.In terms of our understanding of objects in the real word,there may be little disagreement that the real features of an object,whether geometrical or physical, exist together as a coherent whole belonging to one entity in the outside world. The question of how the perceptual system represents perceptual objects as fundamental units of conscious perceptual experience,however,has either given rise to much controversy when considered,or not been considered at all.But the truth remains that real features of a real object,at a given time,originally coexist together rather than being separated;a real object is an integral stimulus,a single thing.This truth is a fundamental property of a real-world object.No one doubts the direct perception of various featural properties such as brightness,colour, line orientation,and so on.Why,then,is only this fundamental property of ``belonging together as a whole''excluded from the membership of primitives in our perceptual world?The assumption that the visual system cannot directly perceive a real integral object has not yet been proved or disproved.Indeed,the continuing challenges to the issues of feature binding suggest that this question deserves closer scrutiny.From the perspective of early holistic registration,the feature-binding pro-blem is an ill-posed question:Not just a question of getting off on a wrong foot but even a question of``standing upside down''.In this sense,the feature-binding problem might be a wrong,inverted question.Kubovy and Pomerantz (1981)commented:``the main problems facing us today are quite similar to those faced by the Gestalt psychologists in the first half of this century''.After half a century,the study of visual perception appears,in some sense,to be back560CHENto square one.This situation leads us to wonder whether the problems of feature binding are due to difficulties in posing the fundamental question of``Where to begin''.Where does the above analysis leave us?It suggests that early holistic registration may provide a way to avoid the feature-binding problem by focusing on issues of perceptual organization.In other words,we may apply the concept of perceptual organization to reverse back the inverted(upside-down)question of feature binding.The topological approachDespite its deep and rational core in the idea of early holistic registration,the notion of perceptual organization has its own problems.In particular,like other Gestalt concepts,it has suffered from a lack of proper theoretical treatment. Gestalt evidence has often been criticized for being mainly phenomenological and relying mainly on conscious experience.Consequently,explanations from theories of perceptual organization usually rely on intuitive or mentalistic concepts that are somewhat vague and elusive.What is needed is a proper formal analysis of perceptual organization that goes beyond intuitive approa-ches,and provides a theoretical basis for describing or defining precisely the core concepts related to perceptual organization,e.g.,``global''vs.``local'', ``objects'',``grouping'',and others.Until the intuitive notions of these Gestalt-inspired concepts become properly and precisely defined,the proposed principles of perceptual organization cannot be entirely testable.Delimiting the concept of perceptual organizationTo give a precise description of the essence of perceptual organization,we first need to properly delimit the concept of perceptual organization.On the one hand,as Rock(1986)pointed out,``The concept of perceptual organization should not be defined so loosely as to be a synonym of perception'';on the other hand,this concept should not be so limited as to be unable to cover the great variety and the commonplace occurrence of perceptual organization.The fol-lowing definition of perceptual organization given by Rock(1986)is considered to define properly the very notion of perceptual organization:The meaning of organization here is the grouping of parts or regions of the field with one another,the``what goes with what''problem,and the differentiation of figure from ground.According to this definition,the study of perceptual organization is concerned with early stages of perceptual processing divorced from high-level cognition, and therefore such delimitation pitches our discussion at the right level to answer the basic question of``Where visual processing begins''.On the other hand,theGLOBAL TO LOCAL TOPOLOGICAL PERCEPTION561 concept of perceptual organization discussed in the present paper deals withgeneral processes,such as figure±ground differentiation,grouping,``what goes with what'',belonging and assignment(not particular processes,such as dif-ferentiating luminance flux,discriminating orientation,or recognizing a face), and with abstract things,such as objects,units,and wholes as well as their counterparts,such as items,elements,and parts(not concrete things,such as a line segments,a geometrical figure,a friend's face).These general processes and abstract things represent the variety and commonplace occurrence of per-ceptual organization.Figure1illustrates this.The percept of Figure1A may be described at a semantic level:Either a vase or two profiles face to face.On the other hand,it may be described in terms of the vocabulary of perceptual orga-nization:Two boundaries(units)grouped into one object(as the basis of the percept of a vase)or two boundaries(units)separated into two objects(as the basis of the percept of the two profiles face to face).It is the latter level,the level of perceptual organization,which our present research focuses on.Furthermore, as Figure1B demonstrates,perceptual organization may be perceived without semantic meaning.Here even though there is little semantic meaning involved in the stimulus,either the black parts are perceived to be an unified whole as a figure and the grey parts,another unified whole as background,or vice versa. Top-down processing of prior knowledge or expectation may influence per-ceptual organization,but it will avoid possible confusion if we consider per-ceptual organization and top-down processing of high-level cognition separately.This strengthens the rationale for defining the terminology for describing perceptual organization,emphasizing the primacy of perceptual organization.Major challenges to establishing a proper theoretical treatment on perceptual organization:Its commonplace,and its general and abstract characteristicsThe concept of perceptual organization reflects the most common fact that the phenomenal world contains objects separated from one another by space or background.Phenomena in perceptual organization are usually so common that they have not been looked on as an achievement of the perceptual system,and, thus,as something to be explained(Rock,1986).For example,tremendous efforts have been made to study how to detect line segments with orientation and location,but little attention has been paid to the question of how to perceive a line segment as a single object.While the study of face recognition has advanced considerably,the fundamental grouping question of``which eyes go with which noses,which noses with which mouths,and so forth''(Pomerantz,1981)has been almost completely ignored.One more example shows how commonplace characteristics of perceptual organization make it difficult to realize that there are problems requiringexplanation.In 1990,Rock and Palmer revealed two primary laws of perceptual organization:``Connectedness''and ``common region'',referring to the pow-erful tendency of the visual system to perceive any connected or enclosed regionas a single unit.The phenomena relating to the two laws are so common andself-evident that even classical Gestalt psychologists failed to realizethat an explanation was required for why elements that are either physically connected or enclosed by a closed curve are perceived as a single unit.As ourdiscussionABFigure 1.(A)An ambiguous figures of ``a vase vs.two faces'',showing competing organization.(B)An example of ambiguous figures,showing competing organization without involving semantic meaning.562CHENGLOBAL TO LOCAL TOPOLOGICAL PERCEPTION563 goes on,we will see that these two Gestalt laws closely approach the preciseformal(topological)description of the essence of Gestalt principles.Never-theless,they were neglected for more than a half a century!Besides the problem of being easily overlooked,one more major challenge to establishing a scientific framework for perceptual organization stems from the abstract and general characteristics of the concept.A theoretical explanation of perceptual organization,to possess explanatory power,must be built on even more general and abstract concepts than this vocabulary.The next question, therefore,is:What kinds of concept are more general and abstract than,for example,``what goes with what'',grouping,belongingness,wholes,and per-ceptual objects,and therefore,suitable for the formal analysis of organizational processes?It is not difficult to see that featural properties commonly used in the feature-analysis approach,such as orientation,distance,and size,cannot help out in dealing with the problems facing us in finding a formal explanation of perceptual organization.Topology provides a formal description of perceptual organization:Insight from invariants over shape-changing transformationsTopology has been considered a promising mathematical tool for providing a formal analysis of concepts and processing of perceptual organization(e.g., Chen,1982,2001).Topology is a branch of mathematics that aims at studying invariant properties and relationships under continuous and one-to-one trans-formations,termed topological transformations.The properties preserved under an arbitrary topological transformation are called topological properties.A topological framework of visual perception can be broad enough to encom-pass the variety of phenomena in perceptual organization,such as``what goes with what'',grouping,belonging,and parsing visual scenes into potential objects,and,on the other hand,precise enough to be free from intuitive approaches.Topology is often considered as one of the most abstract branches of mathematics.If the concepts of topology,their relevance and applicability to perceptual organization are difficult to contemplate in the abstract,an appeal to illustrative examples might be helpful.In the following,I will analyse in some depth three typical cases of perceptual organization to demonstrate why and how to advance the topological approach to perceptual organization.Question1:Figure and ground perceptionÐwhat attributes of stimuli determine the segregation of figure from background?Despite the common acceptance that figure±ground perception is fundamental and occurs at the early stage of perception,and despite the large body of empirical findings about。

基于MIMIC-IV数据库中发生血流感染的危重疾病患者相关数据构建革兰阴性菌血流感染风险预测模型

基于MIMIC-IV数据库中发生血流感染的危重疾病患者相关数据构建革兰阴性菌血流感染风险预测模型

基于MIMIC -IV 数据库中发生血流感染的危重疾病患者相关数据构建革兰阴性菌血流感染风险预测模型陈秋宇1,秦泽辉2,刘享田3,叶莉萍3,田行瀚31 滨州医学院第二临床医学院,山东烟台 264000;2 潍坊医学院附属医院重症医学科;3 烟台毓璜顶医院重症医学科摘要:目的 基于重症医学信息数据库(MIMIC -IV )中发生血流感染的危重疾病患者相关数据构建预测危重疾病患者革兰阴性菌血流感染发生风险预测模型,以期为预测危重疾病患者发生革兰阴性菌血流感染风险提供新的方法。

方法 收集MIMIC -IV 中2 503例发生血流感染的危重疾病患者的临床资料及实验室检查指标[血常规(红细胞、白细胞、血小板等)、血生化(钾离子、钙离子、氯离子、碳酸氢根、阴离子间隙和尿素氮等)、凝血功能指标(INR 、PT 、PTT )]数据。

将所有危重疾病患者以7∶3的比例分为训练集(1 752例)和验证集(751例)。

在训练集中使用LASSO 回归初步筛选出危重疾病患者发生革兰阴性菌血流感染的影响因素,并将筛选出来的影响因素行多因素Logistic 回归分析,建立危重疾病患者革兰阴性菌血流感染风险预测模型(列线图模型)。

在训练集和验证集中通过受试者工作特征曲线(ROC )、校准曲线和决策曲线(DCA )分别对列线图模型的区分度、一致性、临床适用性进行评价。

结果 年龄、患有肿瘤、肝胆系统疾病、嗜酒史、钾离子、钙离子、碳酸氢根、阴离子间隙和尿素氮为危重疾病患者发生革兰阴性菌血流感染风险的影响因素,基于以上影响因素采用Logistic 回归分析,构建列线图模型。

训练集和验证集中,列线图模型预测危重疾病患者发生革兰阴性菌血流感染的ROC 下面积分别为0.711(95% CI 0.667~0.756)、0.705(95% CI 0.678~0.733);校准曲线表明列线图模型预测革兰阴性菌血流感染发生的结果与实际结果之间具有良好的一致性(P = 0.764);DCA 显示列线图模型具有良好的临床适用性。

AI in Infectious Disease Predictions

AI in Infectious Disease Predictions

AI in Infectious Disease PredictionsInfectious diseases have been a significant threat to public health worldwide, causing widespread outbreaks and impacting millions of lives. The emergence of artificial intelligence (AI) technology has revolutionized the field of infectious disease predictions, offering new tools and approaches for early detection, response, and prevention of outbreaks.AI in infectious disease predictions utilizes machine learning algorithms to analyze vast amounts of data, such as clinical records, environmental factors, and pathogen genetics, to identify patterns and trends that can help predict the spread of diseases. By processing this data, AI models can generate real-time forecasts and alerts, enabling healthcare providers and public health officials to take proactive measures to control the spread of infectious diseases.One of the key advantages of AI in infectious disease predictions is its ability to incorporate multiple data sources and variables into predictive models. Traditional disease forecasting methods often rely on historical data and limited variables, which can be insufficient to capture the complexity and dynamics of disease outbreaks. AI algorithms, on the other hand, can analyze a wide range of data in real-time, including social media trends, climate data, and population movements, providing a more comprehensive and accurate picture of the potential risks and trends of infectious diseases.Furthermore, AI in infectious disease predictions can offer more timely and localized information, allowing for targeted interventions and responses. By leveraging data analytics and predictive modeling, AI systems can identify high-risk areas, populations, and transmission patterns, enabling healthcare authorities to allocate resources effectively and implement tailored control measures to contain outbreaks.In addition, AI technology can help improve the speed and accuracy of diagnostics for infectious diseases. By using machine learning algorithms to analyze medical images, genetic sequences, and patient data, AI systems can assist healthcare providers indiagnosing diseases more quickly and accurately, leading to early treatment and containment of infections.Moreover, AI in infectious disease predictions can enhance surveillance and monitoring efforts by automating the analysis of vast amounts of data from various sources. By continuously monitoring and analyzing disease trends, AI systems can provide early warnings of potential outbreaks, enabling rapid response and mitigation strategies.While AI technology holds great promise in transforming infectious disease predictions, there are also challenges and limitations that need to be addressed. Ethical considerations, data privacy issues, and biases in AI algorithms are some of the key concerns that need to be addressed to ensure the responsible and effective use of AI in infectious disease predictions.In conclusion, AI in infectious disease predictions has the potential to revolutionize the way we detect, monitor, and respond to infectious disease outbreaks. By harnessing the power of AI technology, we can improve the effectiveness and efficiency of public health interventions, ultimately reducing the impact of infectious diseases on global health.。

上行视频业务大带宽保障方案

上行视频业务大带宽保障方案

上行视频业务带宽保障【摘要】5G商用进程的全面开启和网络建设的加速推进,进一步推动5G从ToC领域持续向ToB延伸。

5G与垂直行业的进一步融合应用,从更宽范围的业务需求、更丰富的功能、更可靠的性能等方面又对5G 通信网络提出了更高、更严格的要求。

但TOB行业用户存在多路视频监控,每个视频存在I帧和P帧,多路视频I帧碰撞瞬时流量将超过小区容量,导致视频出现卡顿,掉帧等现象。

通过无线侧的I帧离散技术,可以将多路视频的I帧离散开,有效的解决多路视频的I帧碰撞引起的视频卡顿问题。

【关键字】TOB视频卡顿I帧离散技术【任务名称】【在上述任务简称中选择】【背景】一、问题描述高清视频监控、视觉质量检测等操作场景、以8K视频、3D视频、VR/AR为代表的增强带宽业务将对网络带宽产生数百GbPS的超高需求,对回传网络造成巨大传输压力,外场视频远控业务,经常因多路视频I帧碰撞瞬时流量超过小区容量,导致视频出现卡顿,吊帧等现象。

二、分析过程2.1视频网络问题分析(1)高清视频监控、视觉质量检测等操作场景、以8K视频、3D视频、VR/AR为代表的增强带宽.业务将对网络带宽产生Gbps级的超高需求,对上行回传网络造成巨大传输压力,容易导致空口带宽传输受限。

(2)多路视频I帧碰撞瞬时流量将超过小区容量,导致视频出现卡顿,掉帧等现象。

2.2视频I帧、P帧概念视频是利用人眼视觉暂留的原理,通过播放一系列的图片,使人眼产生运动的感觉。

单纯传输视频画面,数据量非常大,对现有的网络和存储来说是不可接受的。

为了能够使视频便于传输和存储,人们发现视频有大量重复的信息,如果将重复信息在发送端去掉,在接收端恢爱出来,这样就大大减少了视频数据的文件,因此有了H.264视频压缩标准。

在H.264压缩标准中I帧、P帧、B帧用于表示传输的视频画面。

1、I帧(Intra-PrediCtiOn)I帧又称帧内编码帧,是一种自带全部信息的独立帧,无需参考其他图像便可独立进行解码,可以简单理解为一张静态画面。

5-氟尿嘧啶无痛微针阵列治疗寻常疣的临床观察

5-氟尿嘧啶无痛微针阵列治疗寻常疣的临床观察

5-氟尿嘧啶无痛微针阵列治疗寻常疣的临床观察茅伟安;张立超;张健;曹蒂莲;李玲;王莉;瞿奕【摘要】目的观察5-氟尿嘧啶无痛微针阵列用于寻常疣治疗的临床疗效及对细胞免疫功能的影响.方法将60例寻常疣患者随机分为无痛微针阵列治疗组(微针治疗组)、涂药组及注射给药组,各20例,应用5-氟尿嘧啶等量用药治疗,每周2次,共12次;分别于治疗前、治疗后、治疗后3个月观察疗效及感染、疼痛、红肿、出血、瘙痒等不良反应和毒副作用.另于治疗前、后分别抽取微针治疗组患者及正常人对照组外周血,测定细胞免疫功能:CD4+、CD8+ T细胞数量(百分比)并进行比较.结果微针治疗组痊愈18例,显效2例,好转0例,无效0例,总有效率为100%;涂药组痊愈8例,显效6例,好转4例,无效2例,总有效率为70%;注射给药组痊愈12例,显效6例,好转2例,无效0例,总有效率为90%.微针治疗组疗效优于涂药组(P<0.05),而与注射给药组比较差异无统计学意义(P>0.05).微针治疗组治疗前与正常人对照组的CD4+、CD8+ T细胞数量及CD4+/CD8+比值差异均有统计学意义;微针治疗后与治疗前比较差异亦有统计学意义.不良反应及复发率均以微针治疗组为最低.结论 5-氟尿嘧啶无痛微针阵列用于寻常疣疗效显著,皮肤刺激性小,复发率低,并能改善细胞免疫功能.【期刊名称】《新疆医科大学学报》【年(卷),期】2013(036)004【总页数】4页(P505-508)【关键词】无痛微针阵列;5-氟尿嘧啶;寻常疣;疗效;细胞免疫功能【作者】茅伟安;张立超;张健;曹蒂莲;李玲;王莉;瞿奕【作者单位】上海市第七人民医院皮肤科,上海,200137【正文语种】中文【中图分类】R752.5+1寻常疣是一种常见的病毒性皮肤病,可导致皮肤功能障碍和形象破坏,影响患者的身心健康。

该疾病的临床治疗方法多样,其中应用5-氟尿嘧啶是一种临床效果较确切且损伤较小的药物疗法。

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Features
• The basic image block unit
– H.264: 4x4 size – MPEG-1/2/4 and H.261/3: 8x8 size
• Some advance features of H.264
– – – – 4x4 integer DCT Intra-prediction in I-frame coding Quarter-pixel motion compensation Multiple reference frame and multiple block size for P-frame coding
Signaling I-Prediction Mode (3-4)
• The encoder sends a flag for each 4x4 block, prev_intra4x4_pred_mode
Signaling I-Prediction Mode (2-4)
• In below figure, C is the current 4x4 block to be encoded
• For current block C, the encoder and decoder calculate the most probable prediction mode, the minimum of the prediction modes of A and B • If either of these neighboring blocks is not available, the corresponding value A or B is set to 2 (DC prediction mode)
Luminance Macroblock in a QCIF Frame
4x4 Intra Prediction (1-2)
• 4x4 block and its neighboring pixels
• 4x4 luma prediction mode
4x4 Intra Prediction (2-2)
• A prediction mode is a way to generate 16 predictive pixel values (named a to p) using some or all of the neighboring pixels A to Q
– Mode 0: Vertical prediction mode in which pixels a, e, i and m are predicted by A – Mode 1: Horizontal prediction mode in which pixels a, b, c and d are predicted by I – Mode 2: DC prediction in which all pixels (a to p) are predicted by (A+B+C+D+I+J+K+L)/8 – The other modes are similar except that the directions are different
– Example of profiling
• Signaling I-Prediction Mode • Conclusion • References
AVC Encoder Overview
Idea issue!
Introduction
• In the upcoming H.264, intra-prediction for each 4x4 block is used to compress I-frame
– However, the full search algorithm to choose one of the 9 prediction modes is computationally expensive
• A novel approach of mode selection
– Mode selection method based on partial computation of the cost function ( Cost16 = SAD16 + 2Rλ(QP) ), early termination and selective computation of highly probable mode – Using the selective search algorithm of each prediction mode
A Novel Approach of Intra-Prediction Mode Selection for 4x4 in H.264
(First_Edition)
2019.07.07 Summer Vacation Seminar Myoung-Seo Kim
Contents
• • • • • AVC Encoder overview Introduction Features Luminance Macroblock in a QCIF Frame 4x4 Intra prediction
Example of profiling
• Prediction iction Mode (1-4)
• The choice of intra prediction mode for each 4x4 block must be signaled to the decoder and this could potentially require a large number of bits • To encode the prediction mode for each 4x4 block efficiently, the correlation between spatially adjacent blocks is exploited probability list!
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