BAG-1及p16在宫颈上皮内瘤变中的诊断价值
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甘肃医药 2019 年 38 卷第 4 期 Gansu Medical Journal袁2019袁Vol.38袁No.4
ቤተ መጻሕፍቲ ባይዱ
窑297窑
BAG-1 及 p16 在宫颈上皮内瘤变中的诊断价值
宋丽娟1 闵建平2 刘玮1 李晓琴1 康雅琼1 李波1 李晓梅1 金荣1 杨荣1
1.甘肃省肿瘤医院，甘肃 兰州 730050；2.甘肃省医学科学研究院，甘肃 兰州 730050
【摘要】 目的：检测宫颈上皮内瘤变（CIN）组织中 BAG-1 及 p16 蛋白的表达，探讨其在 CIN 中的诊断价值。方法：采用免疫组化法 检测正常宫颈组织及 CIN 组织中 BAG-1 及 p16 的表达情况，其中正常宫颈/慢性宫颈炎 30 例、CIN1 及 CIN2/3 各 60 例。结果：BAG-1 及 p16 表达率在正常/慢性宫颈炎组为 3.3%（1/30）及 0，CIN1 组为 36.7%（22/60）及 28.3%（17/60）、CIN2/3 组为 90%（54/60）及 86.7%（52/ 60），二者的表达均随着病变严重程度的上升而增高（字2=50.875 及 103.560，P约0.01），且 CIN2/3 组与 CIN1 组相比，BAG-1 及 p16 的表达 率均明显升高（字2=46.538 及 51.361，P约0.01）。BAG-1 及 P16 诊断区分 CIN1 及 CIN2/3 的灵敏度分别为 90.0%及 86.7%，特异度分别为 63.3%及 71.7%，BAG-1 的阴性预测值（NPV）（86.4%）比 p16（84.3%）略高，而阳性预测值（PPV）（71.1%）比 p16（75.4%）略低。结论：BAG1 对诊断不同级别 CIN 具有较高的灵敏度及特异度，BAG-1 可能作为一种新的生物分子标志物与 p16 联合检测用以辅助诊断 CIN1 及 CIN2/3。
【关键词】 BAG-1；p16；免疫组化；宫颈上皮内瘤变；诊断
中图分类号：R737.33
文献标识码：A
文章编号：1004-2725（2019）4-0297-04
Diagnostic value of BAG-1 and p16 in cervical intraepithelial neoplasia Song Lijuan1袁Min Jianping2袁Liu W ei1袁Li Xiaoqin1袁Kang Y aqiong1袁Li Bo袁Li Xiaomei1袁Jin Rong1袁Y ang Rong1. 1.Gansu Provincial Cancer Hospital袁Lanzhou 730050 China; 2. Gansu Provincial A cademic Institute for Medical Research袁Lanzhou 730050袁China Corresponding author:Y ang Rong袁E-mail: yqq2000115@
【Abstract】 Objective：To detect the expression of BAG -1 and p16 protein in cervical intraepithelial neoplasia （CIN），and to explore its diagnostic value in CIN. Methods: Immunohistochemistry was used to detect the expression of BAG -1 and p16 in normal cervical tissues and CIN tissues，including 30 cases of normal cervix/chronic cervicitis，60 cases of CIN1 and 60 cases of CIN2/3. Results: The expression rates of BAG-1 and p16 were 3.3%（1/30）and 0 in the normal/chronic cervicitis group，and 36.7%（22/60）and 28.3%（17/60） in the CIN1 group，the CIN2/3 group was 90%（54/60）and 86.7%（52/60），both of the expression rates increased with the severity of the lesion （字2=50.875 and 103.560，P约0.01），and the expression rates of BAG-1 and p16 were significantly higher in CIN2/3 group than in CIN1 group （字2=46.538 and 51.361，P约0.01）.The sensitivity of BAG-1 and p16 for differentiating between CIN1 and CIN2/3 were 90.0% and 86.7%， respectively，the specificity was 63.3% and 71.7%，respectively. The negative predictive value （NPV）of BAG-1 （86.4%）was slightly higher than that of p16（84.3%），while the positive predictive value（PPV）（71.1%）was slightly lower than that of p16（75.4%）. Conclusion: BAG-1 has high sensitivity and good specificity for differentiating different levels of CIN. BAG-1 may be used as a new biomolecular marker combined with p16 to assist the diagnosis of CIN1 and CIN2/3.
ቤተ መጻሕፍቲ ባይዱ
窑297窑
BAG-1 及 p16 在宫颈上皮内瘤变中的诊断价值
宋丽娟1 闵建平2 刘玮1 李晓琴1 康雅琼1 李波1 李晓梅1 金荣1 杨荣1
1.甘肃省肿瘤医院，甘肃 兰州 730050；2.甘肃省医学科学研究院，甘肃 兰州 730050
【摘要】 目的：检测宫颈上皮内瘤变（CIN）组织中 BAG-1 及 p16 蛋白的表达，探讨其在 CIN 中的诊断价值。方法：采用免疫组化法 检测正常宫颈组织及 CIN 组织中 BAG-1 及 p16 的表达情况，其中正常宫颈/慢性宫颈炎 30 例、CIN1 及 CIN2/3 各 60 例。结果：BAG-1 及 p16 表达率在正常/慢性宫颈炎组为 3.3%（1/30）及 0，CIN1 组为 36.7%（22/60）及 28.3%（17/60）、CIN2/3 组为 90%（54/60）及 86.7%（52/ 60），二者的表达均随着病变严重程度的上升而增高（字2=50.875 及 103.560，P约0.01），且 CIN2/3 组与 CIN1 组相比，BAG-1 及 p16 的表达 率均明显升高（字2=46.538 及 51.361，P约0.01）。BAG-1 及 P16 诊断区分 CIN1 及 CIN2/3 的灵敏度分别为 90.0%及 86.7%，特异度分别为 63.3%及 71.7%，BAG-1 的阴性预测值（NPV）（86.4%）比 p16（84.3%）略高，而阳性预测值（PPV）（71.1%）比 p16（75.4%）略低。结论：BAG1 对诊断不同级别 CIN 具有较高的灵敏度及特异度，BAG-1 可能作为一种新的生物分子标志物与 p16 联合检测用以辅助诊断 CIN1 及 CIN2/3。
【关键词】 BAG-1；p16；免疫组化；宫颈上皮内瘤变；诊断
中图分类号：R737.33
文献标识码：A
文章编号：1004-2725（2019）4-0297-04
Diagnostic value of BAG-1 and p16 in cervical intraepithelial neoplasia Song Lijuan1袁Min Jianping2袁Liu W ei1袁Li Xiaoqin1袁Kang Y aqiong1袁Li Bo袁Li Xiaomei1袁Jin Rong1袁Y ang Rong1. 1.Gansu Provincial Cancer Hospital袁Lanzhou 730050 China; 2. Gansu Provincial A cademic Institute for Medical Research袁Lanzhou 730050袁China Corresponding author:Y ang Rong袁E-mail: yqq2000115@
【Abstract】 Objective：To detect the expression of BAG -1 and p16 protein in cervical intraepithelial neoplasia （CIN），and to explore its diagnostic value in CIN. Methods: Immunohistochemistry was used to detect the expression of BAG -1 and p16 in normal cervical tissues and CIN tissues，including 30 cases of normal cervix/chronic cervicitis，60 cases of CIN1 and 60 cases of CIN2/3. Results: The expression rates of BAG-1 and p16 were 3.3%（1/30）and 0 in the normal/chronic cervicitis group，and 36.7%（22/60）and 28.3%（17/60） in the CIN1 group，the CIN2/3 group was 90%（54/60）and 86.7%（52/60），both of the expression rates increased with the severity of the lesion （字2=50.875 and 103.560，P约0.01），and the expression rates of BAG-1 and p16 were significantly higher in CIN2/3 group than in CIN1 group （字2=46.538 and 51.361，P约0.01）.The sensitivity of BAG-1 and p16 for differentiating between CIN1 and CIN2/3 were 90.0% and 86.7%， respectively，the specificity was 63.3% and 71.7%，respectively. The negative predictive value （NPV）of BAG-1 （86.4%）was slightly higher than that of p16（84.3%），while the positive predictive value（PPV）（71.1%）was slightly lower than that of p16（75.4%）. Conclusion: BAG-1 has high sensitivity and good specificity for differentiating different levels of CIN. BAG-1 may be used as a new biomolecular marker combined with p16 to assist the diagnosis of CIN1 and CIN2/3.