SPRED1蛋白使用说明书
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Instruction manual
本产品仅供研究使用,不用于临床诊断。
本公司提供的电子版本说明书仅供参考,实验请以收到的纸质手册为准。
SPRED1, 1-444aa, Human, His tag, E.coli
产品货号: TP04022
第三版别名:Sprouty-related, EVH1 domain-containing protein 1, hSpred1, NFLS, spred-1
描述:SPRED1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. This protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). Recombinant human SPRED1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
配方:Liquid. In 20mM Tris-HCl (pH 8.0) containing 10% glycerol
分子量:52.9 kDa (467aa)
序列: MGSSHHHHHHSSGLVPRGSHMGSMSEETATSDNDNSYARVRAVVMTRDDSSGGWLPLGGSGLSSVTVFKVPHQEENG CADFFIRGERLRDKMVVLECMLKKDLIYNKVTPTFHHWKIDDKKFGLTFQSPADARAFDRGIRRAIEDISQGCPESKNEAE GADDLQANEEDSSSSLVKDHLFQQETVVTSEPYRSSNIRPSPFEDLNARRVYMQSQANQITFGQPGLDIQSRSMEYVQRQIS KECGSLKSQNRVPLKSIRHVSFQDEDEIVRINPRDILIRRYADYRHPDMWKNDLERDDADSSIQFSKPDSKKSDYLYSCGDE TKLSSPKDSVVFKTQPSSLKIKKSKRRKEDGERSRCVYCQERFNHEENVRGKCQDAPDPIKRCIYQVSCMLCAESMLYHC MSDSEGDFSDPCSCDTSDDKFCLRWLALVALSFIVPCMCCYVPLRMCHRCGEACGCCGGKHKAAG
纯度:> 95% by HPLC
浓度:0.5 mg/ml (determined by Bradford assay)
内毒素:<1.0 EU per 1 ug of protein (determined by LAL method)
存储: +4°C 保存 (1-2 周). 长期保存在-20°C或者-70°C. 避免反复冻融.
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