LC-MS常见本底离子

338.3
350 400 m/z, amu
XIC of +EMS: Exp 1, 362.1 to 362.6 amu from Sample 12 (CLZ-016_0-8h) of 20101206.wiff (Turbo Spray...
.
.
.
Max. 2.4e8 cps.
2.4e8 2.0e8
17.86
17.56
14 16 240 275 Time, min +EMS: Exp 1, 17.883 min from Sample 12 (CLZ-016_0-8h) of 20101206.wiff (Turbo Spray)
0.00
19.14 2 35 4 69 6 103 8 137 10 171 12 206 18 310 20 345 22 380 24 415 26 449 28 484
28 492
.
.
Max. 6.2e6 cps.
.
6.0e6 4.0e6 2.0e6 133.3
.
362.2
I
n
t
e
149.2 150
189.4
400 450 500 m/z, amu +EPI (362.27) Charge (+1) CE (45) FT (20): Exp 2, 15.893 min from Sample 9 (ZWP-003_0-8h) of 20101...
t
M2’ 15.82
15.27 14.90 2 35 4 69 6 103 8 137 10 171 12 206 18 310 20 345 22 380 24 415 26 449 28 484
1.0e8 0.0
I
14 16 240 275 Time, min +EMS: Exp 1, 15.867 min from Sample 12 (CLZ-016_0-8h) of 20101206.wiff (Turbo Spray)
15.81
5.00e7 0.00
I
14 16 243 279 Time, min +EMS: Exp 1, 15.857 min from Sample 9 (ZWP-003_0-8h) of 20101206.wiff (Turbo Spray)
n
18 314
20 349
22 385
24 420
26 456
20 349
22 385
24 420
26 456
28 492
.
.
Max. 4.1e7 cps.
.
4.0e7 3.0e7 2.0e7 1.0e7 133.3 100 149.3 150 188.3 197.5 200
I n t
361.2
e
416.5 443.0 468.3 477.5 400 450 500 m/z, amu +EPI (361.31) Charge (+1) CE (45) FT (20): Exp 2, 17.920 min from Sample 9 (ZWP-003_0-8h) of 201012...
.
.
.
Max. 6.0e8 cps.
6.0e8 4.0e8
I t
Parent
18.25
2.0e8 14 16 240 275 Time, min +EMS: Exp 1, 17.940 min from Sample 12 (CLZ-016_0-8h) of 20101206.wiff (Turbo Spray) 0.0 2 35 4 69 6 103 8 137 10 171 12 206 18 310 20 345 22 380 24 415 26 449 28 484
M2 2.35 Isotope of parent
700 600 500 400 300 200 100 2 196 Time, min XIC of +MRM (3 pairs): 361.0/188.0 amu ... 2.43 0 1 99 2.79 3.16 3.45 3 294 Max. 320.0 cps. 3.69
413.5 455.2 482.2 498.1 557.6 400 450 500 550 m/z, amu +EPI (375.37) Charge (+1) CE (45) FT (20): Exp 2, 17.977 to 19.812 min from Sample 12 (CLZ-016_0-8h)...
1.5e5
2.45 1.0e5
5.0e4
2 196 Time, min XIC of +MRM (3 pairs): 361.0/188.0 amu ...
0.0
1 99
3 294 Max. 1.0e6 cps. 2.45
parent
1.00e6
In te n s ity , c p s
In te n s ity , c p s
XIC of +EMS: Exp 1, 361.0 to 361.5 amu from Sample 12 (CLZ-016_0-8h) of 20101206.wiff (Turbo Spray...
.
.
.
Max. 1.3e7 cps.
M3’
1.30e7 1.00e7 5.00e6
I n t e
I n t
188.2
e
145.2 150
199.3 200
253.1 250
272.2
318.2 300
344.2
361.3 450 500 550 600 650 700
350 400 m/z, amu
Blank urine sample
XIC of +MRM (3 pairs): 362.0/189.0 amu ... 790
. .
100
193.4 207.6 237.5 200 250
279.2
363.5
413.4
432.4
473.3 504.3 547.4 500 550
581.4 595.5 600 650 700
Max. 2.0e6 cps.
2.0e6 1.5e6 1.0e6 5.0e5 66.0 50 138.2 100
XIC of +MRM (3 pairs): 362.0/189.0 amu ... 790
In te n s ity , c p s
ZWP-003 0-8hr urine sample
Max. 790.0 cps. XIC of +MRM (3 pairs): 362.0/189.0 amu ... Max. 1.5e5 cps.
In te n s ity , c p s
ZWP-003 0-8hr urine sample
Max. 790.0 cps. XIC of +MRM (3 pairs): 362.0/189.0 amu ... 1.9e5
In te n s ity , c p s
Max. 1.9e5 cps.
2.35
XIC of +EMS: Exp 1, 362.0 to 362.5 amu from Sample 9 (ZWP-003_0-8h) of 20101206.wiff (Turbo Spray)
.
.
.
Max. 1.2e8 cps.
17.88
1.00e8
t
e
M2
15.19 2 35 4 70 6 104 8 138 10 173 12 208
. .
Max. 7.0e6 cps.
202.2
6.0e6
n
4.0e6 2.0e6 78.1 50 135.3 100 145.4 152.4 150 161.4 213.4 229.4 200 250 299.3 300 332.3
I
t
e
358.2 375.2 450 500 550 600 650 700
.
.
n
e
Max. 1.7e7 cps.
.
375.2
1.5e7
n
1.0e7 5.0e6 111.2 100 149.2 161.3 202.5 150 200 235.4 250 279.2 305.4 332.5 358.6 300 350
.
I
t
e
397.3 581.4 599.6 624.9 669.7 600 650 700
520.4
561.4 550
400 450 500 m/z, amu +EPI (362.33) Charge (+1) CE (45) FT (20): Exp 2, 15.562 to 15.620 min from Sample 12 (CLZ-016_0-8h)...
. .
585.9 600
.
.
n
e
Max. 2.4e7 cps.
.
2.4e7 2.0e7
n
362.3
1.5e7
I
1.0e7 5.0e6 100 133.4 177.4 189.4 201.4 150 200 279.3 250 319.3 300
.
t
e
345.2 350
365.1 391.3 415.6 459.5 503.3
650
700
Max. 3.5e6 cps.
3.5e6 3.0e6
n
189.2
2.0e6 1.0e6 66.0 78.0 117.1 50 100 145.1 150 154.3 200.1 200 223.2 250 299.2 319.3 300
I
t
e
345.2 362.2 350 400 m/z, amu 450 500 550 600 650 700
. .
100
207.3265.4 200 250
279.3 300
345.2
364.0 377.2 350
415.5 459.3
503.6
520.6 561.4 550
586.2 600
652.4 650
700
Max. 5.6e6 cps.
5.6e6 4.0e6 2.0e6
I n t
189.2
e
66.0 90.0 133.1 50 100
.
.
Max. 3.2e6 cps.
.
391.2 361.3
1.0e6 109.3
.
I
n
2.0e6 348.2 316.4 300 350
t
3.0e6
e
149.3 150
400 450 m/z, amu +EPI (361.27) Charge (+1) CE (45) FT (20): Exp 2, 17.920 min from Sample 12 (CLZ-016_0-8h) of 20101...
17.87 17.58
14 16 243 279 Time, min +EMS: Exp 1, 17.883 min from Sample 9 (ZWP-003_0-8h) of 20101206.wiff (Turbo Spray)
e
2 35
4 70
6 104
8 138
10 173
12 208
18 314
300 250 200 150 100 50 0 1 99 2.38 3.11 3.60 3.70
8.00e5 ቤተ መጻሕፍቲ ባይዱ.00e5 4.00e5 2.00e5
2.21 2 196 Time, min 3 294 0.00 1 99 2 196 Time, min 3 294
Blank urine sample
. . .
318.4 344.5 253.5 279.5 250 300 350
383.4
541.3 550
581.5 600
650
700
Max. 1.7e7 cps.
188.3
1.5e7
n
1.0e7 5.0e6 78.1 50 145.4 138.4 130.1 100 150
I
t
e
344.2 199.3 220.3 200 215.3 279.2 250 318.2 292.1 300 344.9 361.2 350 400 m/z, amu 450 500 550 600 650 700
2.35 1.4e5
In te n s ity , c p s
M2 2.35
1.2e5 1.0e5 8.0e4 6.0e4 4.0e4 2.0e4 2 196 Time, min XIC of +MRM (3 pairs): 361.0/188.0 amu ... 0.0 1 99 3 294 Max. 5.2e5 cps.
145.1 150
200.2 200
223.1 250
299.3
319.2
345.2 362.2 350 400 m/z, amu 450 500 550 600 650 700
300
XIC of +EMS: Exp 1, 375.1 to 375.6 amu from Sample 12 (CLZ-016_0-8h) of 20101206.wiff (Turbo Spray...
XIC of +EMS: Exp 1, 361.0 to 361.5 amu from Sample 9 (ZWP-003_0-8h) of 20101206.wiff (Turbo Spray...
.
.
.
Max. 2.7e8 cps.
Parent
2.7e8 2.0e8 1.0e8 0.0
I n t